14 results about "Nucleic acid amplification technique" patented technology

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.

The present disclosure provides methods and systems for processing a nucleic acid sample. The present disclosure also provides methods and systems for detecting (e.g., optically detecting) a pathogen in a sample. The methods provided herein may use nucleic acid amplification.

The application relates to the field of nucleic acid amplification technology and provides a full-automatic nucleic acid amplification system integrated with microwave temperature control and a working method thereof.The system comprises a microprocessor, a temperature heating module, a nucleic acid amplification container, a microwave generator connected with the microprocessor and a thermocouple, the microwave generator is connected with the temperature heating module, the temperature heating module adopts a planar microwave resonator or a waveguide, the microwave generator is used for outputting microwave signals with specific frequency and power, the temperature heating module is used for directing the microwave signals to nucleic acid amplification reagents in the nucleic acid amplification container to heat the temperature of the nucleic acid amplification reagents, the thermocouple is used for monitoring the temperature of the nucleic acid amplification reagents and uploading the temperature to the microprocessor, and the microprocessor is used for controlling the frequency and power of the microwave signals according to the temperature of the nucleic acid amplification reagents.The temperature can be rapidly raised, the sample volume is reduced, and the nucleic acid amplification time is shortened.

The invention relates to a suction-driven micro-fluidic device for real-time nucleic acid detection, provides an RNA (Ribonucleic Acid) detection system and application thereof, and in particular relates to a dual-nuclease-assisted isothermal cycle amplification integrated detection system and an RNA detection method based on the detection system. The detection system is composed of an initial reaction based on Cas13a nuclease and a cyclic amplification reaction based on a PDANSs biosensor. The initial reaction and the cyclic amplification reaction can be integrated in a single reaction cavity, the dependence on the nucleic acid amplification technology is eliminated, and the integrated RNA quantitative detection system is high in sensitivity and specificity.

The invention discloses a four-channel centrifugal micro-fluidic nucleic acid amplification chip with closed cavities, and relates to the technical field of micro-fluidic cores. Comprising a chip main body, a plurality of detection units are uniformly arranged along the circumference, each detection unit comprises a plurality of adhesive film valves, a plurality of amplification cavities, a plurality of quantitative cavities, a liquid injection cavity and a waste liquid cavity, the liquid injection cavities and the waste liquid cavities are communicated with the quantitative cavities, the adjacent quantitative cavities are communicated, each quantitative cavity is communicated with one adhesive film valve through a quantitative cavity liquid outlet channel, and each quantitative cavity is communicated with the other adhesive film valve through a quantitative cavity liquid outlet channel. The glue film valve is communicated with the amplification cavity through the amplification cavity liquid inlet flow channel, the cold aluminum with glue is arranged in the glue film valve, external force extrudes the glue film valve, glue in the cold aluminum with glue blocks a liquid outlet of the glue film valve, and therefore the amplification cavity is sealed. The device is low in manufacturing cost, can automatically close the chamber, ensures that liquid in the chamber does not leak in the amplification process, is particularly suitable for popularization and application in the field of instant inspection, and is suitable for two nucleic acid amplification technologies of isothermal amplification and variable-temperature amplification.

The invention relates to the technical field of immunodetection, and discloses an immunodetection method and system based on a piezoelectric biosensor, and the method comprises the following steps: under the condition that an antigen to be detected in the piezoelectric biosensor is combined with a capture antibody, adding a connecting antibody and DNA into the piezoelectric biosensor; dNA is amplified through a nucleic acid amplification technology; and analyzing the to-be-detected antigen according to an electric signal acquired by the piezoelectric biosensor after DNA amplification. According to the invention, the specificity of immunodetection and the ultrahigh sensitivity of nucleic acid detection are connected through the piezoelectric biosensor, and a direct signal which is originally weak and is easily submerged by noise can be converted and amplified into a strong output signal with extremely high specificity, so that the signal-to-noise ratio and the target substance detection sensitivity are greatly improved; and the detection limit of the piezoelectric biosensor is further reduced.

This invention belongs to the field of gene detection technology, specifically relating to a kit and detection method for rapid detection of gene variations based on click chemistry and a light-up click ligation probe. The light-up click ligation probe in the kit consists of two oligonucleotide fragments, head-to-tail linked and complementary to the target sequence: a 5'-terminal oligonucleotide fragment and a 3'-terminal oligonucleotide fragment complementary to the target sequence. Both the 3'-end of the 5'-terminal oligonucleotide fragment and the 5'-end of the 3'-terminal oligonucleotide fragment are labeled with click chemistry functional groups. When the target sequence is present, during nucleic acid amplification, the target sequence triggers a click chemistry reaction, and the light-up click ligation probe, complementary to the target sequence, releases a fluorescent signal, enabling real-time parallel synchronous detection of wild-type and mutant genes of the target gene in a single reaction tube or at a single detection point. This invention is applicable to various nucleic acid amplification technologies and possesses the ability to accurately identify single-base variations.

The invention relates to a method for detecting changes of nucleotide sequences by using an isothermal nucleic acid amplification technology, a primer set for detecting selected genetic variants and components of a reaction mixture.

A method and system comprising a programmed computer that can be used to improve nucleic acid analyte detection using real-time amplification and monitoring, the method and system capable of discriminating closely related nucleic acid sequences from one another. In one embodiment, the detection of target sequences in which single nucleotides are different from each other was discriminated by applying a mathematical transformation. In another embodiment, two target sequences in which single nucleotides are different from each other were detected and discriminated from one another using a labeled probe specific to only one of the two target sequences.

The invention discloses application of a synovial fluid source exosome miRNA marker in an osteoarthritis diagnosis product, and belongs to the field of biological medicine detection. The synovial fluid source exosome miRNA marker is miR-3196, and the reagents comprise reagents for detecting the expression level of the synovial fluid source exosome miRNA marker miR-3196 by a sequencing technology, a nucleic acid hybridization technology and a nucleic acid amplification technology. Experimental verification finds that miR-3196 is remarkably low in expression in a synovial fluid exosome of an OA patient, after the miR-3196 low in expression in the exosome is taken by cartilage cells, related signal channels are activated and degradation of the cartilage cells is promoted by regulating MAPK15 in the cartilage cells in a targeted mode, and the miR-3196 can be applied to early clinical diagnosis of osteoarthritis.

The application discloses a double-detection self-powered sensor for detecting sugarcane diseases, application and a detection method thereof, and comprises a biological anode based on nucleic acid amplification product M, a biological cathode based on Au@MB and a self-powered sensor constructed by an electrolyte; wherein the electrolyte comprises glucose, methylene blue PBS buffer and a photothermal test solution. The self-powered sensor can efficiently amplify the nucleic acid of the pathogen of sugarcane smut and shoot rot through a nucleic acid amplification technology, so that the sensor can detect the pathogen at a low concentration, and the missing detection rate is effectively reduced. Meanwhile, multi-mode synergistic detection (electrochemistry, colorimetry and photothermal) further improves the accuracy and specificity of detection, avoids the misjudgment caused by signal cross interference in the traditional method, and ensures the reliability of the detection result.

The application provides a kit for detecting common pathogenic bacteria of otitis media based on a multi-enzyme constant temperature rapid nucleic acid amplification technology, which comprises a first primer pair for detecting Streptococcus pneumoniae, a second primer pair for detecting Haemophilus influenzae, a third primer pair for detecting Moraxella catarrhalis, a fluorescent probe combination and a colloidal gold probe combination; the nucleotide sequences of the first primer pair are SEQ ID NO: 2 and SEQ ID NO: 4 respectively, the nucleotide sequences of the second primer pair are SEQ ID NO: 9 and SEQ ID NO: 10 respectively, and the nucleotide sequences of the third primer pair are SEQ ID NO: 13 and SEQ ID NO: 18 respectively. The application provides a triple multi-enzyme constant temperature rapid synchronous detection kit and a detection method, which have high sensitivity, high specificity and convenient operation, and meet the requirements of rapid diagnosis on the spot in a clinic.

The application discloses a method, a probe, a kit and application thereof for DNA single nucleotide variation detection. The method greatly expands the detection window for distinguishing single nucleotide variation in double-stranded DNA through the conversion of the quantitative relationship between the user-definable detection signal and the target concentration. Through computer simulation and experimental verification, the effectiveness of the method for expanding the detection window and improving sequence selectivity is proved. Since the method directly acts on dsDNA, it is easy to be applied to nucleic acid amplification technology, such as polymerase chain reaction (PCR). The practicability of the method is proved by detecting infection and screening drug resistance of clinical parasite samples collected from rural areas in Honduras. In addition, the method does not need to use high-cost reagents such as enzymes and has relatively high requirements on reaction conditions, so that the method is simple in operation, low in cost and convenient for enterprise and laboratory application.

This invention relates to methods and compositions for detecting a polymorphism in a target nucleic acid sequence using isothermal nucleic acid amplification. More specifically, the present invention relates to using recombinase polymerase amplification (RPA) or Nicking and Extension Amplification Reaction (NEAR) to detect single nucleotide polymorphisms in a target nucleic acid sequence.