5 results about "Endoenzyme" patented technology

The invention provides application of tetrathiomolybdate in preparation of drugs for inhibiting inflammatory factor storm, and belongs to the technical field of drugs for treating inflammatory factor storm and respiratory system diseases. The general molecular formula of the tetrathiomolybdate is X < + > MoS < 42-Y < + >, and the positions of ionic bonds of four sulfur ions of the tetrathiomolybdate are interchangeable; x + and Y + are the same or different cations; the positive ions are inorganic positive ions and / or organic positive ions which can be dissociated in a solution to generate MoS < 2-> or can be subjected to enzymolysis in a biological system to generate MoS < 2->. The invention discloses unique advantages of a single drug scheme in inflammatory factor storm and respiratory system related diseases, especially bacterial acute respiratory system inflammation, for the first time, and tetrathiomolybdate is used as a single active component for preparing the drug for treating bacterial pneumonia and non-viral acute lung injury / acute respiratory distress syndrome.

The invention discloses laccase and application thereof, and belongs to the field of bioengineering. The high-activity laccase Lac-11 is obtained through database mining, and compared with existing high-activity laccase BstCotA, the high-activity laccase Lac-11 has the following beneficial effects that 1, the enzyme activity is remarkably improved, the enzyme activity is improved by nearly 50% under the conditions that the temperature is 37 DEG C and the pH is 5.0, and the industrial enzyme cost is reduced; 2, the pH stability is higher, the enzyme activity retention rate is higher in the pH range of 5.0-8.0, and the method is suitable for wider acid-base environments. And 3, the thermal stability is better, the stability at the medium-high temperature interval of 50-80 DEG C is obviously higher than that of a control enzyme, and the method is suitable for an industrial high-temperature process. 4, the dye decolorization efficiency is higher, the decolorization rates of amino black 10B and crystal violet respectively reach 31% and 37%, and printing and dyeing wastewater can be efficiently treated. And 5, the lignin degradation capacity is outstanding, the degradation rates within 1 hour, 2 hours and 24 hours are 22%, 25% and 58% respectively, and pulping bleaching and biomass recycling are facilitated.

A cellulose-degrading bacterium (Trichoderma erinaceum) A18 and a culture medium and a preparation method of the culture medium, the bacterium is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No. 65616, the bacterium is screened from soil and fallen leaves in Nanning City. The culture medium comprises a seed culture medium and an enzyme production culture medium, components of the seed culture medium are as follows: sodium carboxymethyl cellulose 10 g / L, protein peptone 3 g / L, potassium dihydrogen phosphate 4 g / L, magnesium sulfate heptahydrate 0.3 g / L and calcium chloride 0-0.3 g / L; components of the enzyme production culture medium are as follows: sodium carboxymethyl cellulose 8-12 g / L, protein peptone 2-3 g / L, yeast powder 0.2-3 g / L, ammonium sulfate 0.2-2 g / L, potassium dihydrogen phosphate 4 g / L, magnesium sulfate heptahydrate 0.3 g / L and zinc chloride 0.002 g / L. After the seed culture medium and the enzyme production culture medium are configured with distilled water, the seed culture medium and the enzyme production culture medium are placed in a high-pressure sterilization pot and sterilized at 121 DEG C, inoculation is carried out according to a proportion, and anaerobic culture is carried out in a closed state. The cellulose-degrading bacterium A18 can effectively improve cellulose endoenzyme activity by using the application.

The present application provides a high-temperature mannanase and its application, and the amino acid sequence thereof is SEQ ID NO:1. The mannanase of the present application has the strongest hydrolysis effect on konjac gum, and can also hydrolyze guar gum, has a wide temperature and pH range, the enzyme activity is maintained at 95% or above in the range of 45-75 DEG C, the enzyme activity is maintained at 65% or above in the range of pH 5.5-8.5, and various common metal ions can promote the enzyme activity or have little effect on the activity. The enzyme is a beta-mannanase with endo-activity, and has a good application prospect in feed processing, preparation of manno-oligosaccharides, biological polishing, utilization of agricultural waste, extraction of plant active substances and the like under medium-high temperature conditions.

The invention discloses a signal amplification system for detecting mitochondrial enzyme activity and application. The signal amplification system comprises a depurination and depyrimidine endonuclease 1 response module, an rRNA activation module and a signal report and amplification module. The depurination and depyrimidine endonuclease 1 response module contains a DNA duplex AP-I of an AP site, wherein the DNA duplex AP-I can be specifically recognized and cut by the depurination and depyrimidine endonuclease 1; the rRNA activation module contains a hairpin probe R-H1 of a mitochondrial rRNA recognition sequence; and the signal reporting and amplifying module comprises a fuel hairpin H2 marked with a fluorescence reporting group and a quenching group. The system provided by the invention successfully realizes real-time and in-situ imaging of APE1 enzyme activity in living cell mitochondria, and provides a powerful tool for researching related life processes at a subcellular level.