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1 results about "Endoenzyme" patented technology

An endoenzyme, or intracellular enzyme, is an enzyme that functions within the cell in which it was produced. Because the majority of enzymes fall within this category, the term is used primarily to differentiate a specific enzyme from an exoenzyme. It is possible for a single enzyme to have both endoenzymatic and exoenzymatic functions; for example, glycolytic enzymes of Kreb's Cycle. In most cases the term endoenzyme refers to an enzyme that binds to a bond 'within the body' of a large molecule - usually a polymer. For example an endoamylase would break down large amylose molecules into shorter dextrin chains. On the other hand, an exoenzyme removes subunits from the polymer one at a time from one end; in effect it can only act at the end ponts of a polymer. An exoamylase would therefore remove one glucose molecule at a time from the end of an amylose molecule.

High temperature mannanase and use thereof

The present application provides a high-temperature mannanase and its application, and the amino acid sequence thereof is SEQ ID NO:1. The mannanase of the present application has the strongest hydrolysis effect on konjac gum, and can also hydrolyze guar gum, has a wide temperature and pH range, the enzyme activity is maintained at 95% or above in the range of 45-75 DEG C, the enzyme activity is maintained at 65% or above in the range of pH 5.5-8.5, and various common metal ions can promote the enzyme activity or have little effect on the activity. The enzyme is a beta-mannanase with endo-activity, and has a good application prospect in feed processing, preparation of manno-oligosaccharides, biological polishing, utilization of agricultural waste, extraction of plant active substances and the like under medium-high temperature conditions.
Owner:THIRD INSTITUTE OF OCEANOGRAPHY STATE OCEANI C ADMINISTRATION