The present application provides a high-temperature mannanase and its application, and the
amino acid sequence thereof is SEQ ID NO:1. The mannanase of the present application has the strongest
hydrolysis effect on konjac gum, and can also hydrolyze
guar gum, has a wide temperature and
pH range, the
enzyme activity is maintained at 95% or above in the range of 45-75 DEG C, the
enzyme activity is maintained at 65% or above in the range of pH 5.5-8.5, and various common
metal ions can promote the
enzyme activity or have little effect on the activity. The enzyme is a beta-mannanase with endo-activity, and has a good application prospect in
feed processing, preparation of manno-oligosaccharides, biological
polishing, utilization of
agricultural waste, extraction of
plant active substances and the like under medium-high temperature conditions.