A preparation method of yeast-derived active polypeptide

A technology of active peptides and sources, which is applied in the field of preparation of yeast-derived active peptides, can solve the problems of not effectively removing nucleic acid impurities, easily causing gout and urinary calculi, etc., and achieve the effects of improving purity, obvious biological activity, and shortening the process flow

Inactive Publication Date: 2011-12-21
SOUTH CHINA UNIV OF TECH +1
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These preparation methods adopt yeast protein extraction and enzymatic hydrolysis step by step, and the enzymes used are bromelain, alkaline protease, trypsin and other single enzymes; the nucleoprotein content in yeast protein is relatively high, and the existing preparation methods do not effectively remove nucleic acid impurities. After the human body takes in too much nucleic acid, it will accumulate nucleic acid metabolite uric acid in the body, which can easily cause diseases such as gout and urinary stones.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Extraction and enzymatic hydrolysis of yeast protein: dry yeast powder and water were mixed at a solid-to-liquid ratio of 1: 5 g / mL, and 10% wt of neutral endopeptidase and 10% of leucine aminopeptidase were selected Enzyme hydrolysis of wt and flavor protease 80% wt. The amount of enzyme added was 1% wt of dry yeast powder, the enzymolysis temperature was 45°C, and the enzymolysis time was 2h.

[0024] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution is inactivated at 90°C and then filtered, and the liquid phase components are added to 4 times (V / V) ethanol to remove polysaccharides and nucleic acid impurities.

[0025] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 5kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides.

[0026] (4) Iden...

Embodiment 2

[0028] (1) Extraction and enzymatic hydrolysis of yeast protein: dry yeast powder and water were mixed at a solid-to-liquid ratio of 1: 10 g / mL, and 20% wt of chymotrypsin, 20% wt of leucine aminopeptidase and 60 flavor protease were selected % wt mixed enzyme enzymatic hydrolysis. The amount of enzyme added is 0.5%wt of dry yeast powder, the enzymolysis temperature is 35°C, and the enzymolysis time is 3h.

[0029] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution was inactivated at 100°C and then centrifuged, and the liquid phase components were added with 2 times (V / V) ethanol to remove the polysaccharide and nucleic acid impurities.

[0030] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 8kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides.

[00...

Embodiment 3

[0033] (1) Extraction and enzymatic hydrolysis of yeast protein: mix dry yeast powder and water at a solid-to-liquid ratio of 1: 15 g / mL, and select neutral endopeptidase 30% wt, carboxypeptidase 30% wt and flavor Protease 40% wt mixed enzyme enzymatic hydrolysis. The amount of enzyme added was 2.5% wt of dry yeast powder, the enzymatic hydrolysis temperature was 20°C, and the enzymatic hydrolysis time was 4 hours.

[0034] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution was inactivated at 110°C and then centrifuged, and the liquid phase components were added to 6 times (V / V) ethanol to remove the polysaccharide and nucleic acid impurities.

[0035] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 10kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method of active polypeptide derived from yeast. Mix yeast powder and water, add a mixed enzyme consisting of endoenzyme, exoenzyme, and flavor enzyme, and separate solid and liquid after enzymatic hydrolysis. The polysaccharide and nucleic acid impurities in the liquid phase components were removed by ethanol precipitation. The supernatant is filtered and separated by an ultrafiltration membrane with a molecular weight cut-off of 5kDa to 10kDa, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain the active polypeptide. Through in vitro activity determination, the yeast-derived active polypeptide has blood pressure-lowering, anti-oxidation and blood-sugar-lowering activities.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a method for preparing yeast-derived active polypeptides. Background technique [0002] Health is the foundation of all-round development of human beings. With the development of science and technology and the improvement of living standards, health has become the ultimate goal pursued by human beings in the 21st century. Functional foods for the purpose of disease prevention and health maintenance are therefore more and more popular. Favored by more and more people, the functional food industry will surely become the main artery of the development of the food industry and drive the economic development of related industries. [0003] As an important functional food, bioactive peptides are widely used in medicine, health care, food, cosmetics and other industries because of their significant physiological and pharmacological activities. Since the 1990s, peptide products have r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 胡松青倪贺李琳郭莎莎张才军叶就
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap