A preparation method of yeast-derived active polypeptide
A technology of active peptides and sources, which is applied in the field of preparation of yeast-derived active peptides, can solve the problems of not effectively removing nucleic acid impurities, easily causing gout and urinary calculi, etc., and achieve the effects of improving purity, obvious biological activity, and shortening the process flow
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Embodiment 1
[0023] (1) Extraction and enzymatic hydrolysis of yeast protein: dry yeast powder and water were mixed at a solid-to-liquid ratio of 1: 5 g / mL, and 10% wt of neutral endopeptidase and 10% of leucine aminopeptidase were selected Enzyme hydrolysis of wt and flavor protease 80% wt. The amount of enzyme added was 1% wt of dry yeast powder, the enzymolysis temperature was 45°C, and the enzymolysis time was 2h.
[0024] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution is inactivated at 90°C and then filtered, and the liquid phase components are added to 4 times (V / V) ethanol to remove polysaccharides and nucleic acid impurities.
[0025] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 5kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides.
[0026] (4) Iden...
Embodiment 2
[0028] (1) Extraction and enzymatic hydrolysis of yeast protein: dry yeast powder and water were mixed at a solid-to-liquid ratio of 1: 10 g / mL, and 20% wt of chymotrypsin, 20% wt of leucine aminopeptidase and 60 flavor protease were selected % wt mixed enzyme enzymatic hydrolysis. The amount of enzyme added is 0.5%wt of dry yeast powder, the enzymolysis temperature is 35°C, and the enzymolysis time is 3h.
[0029] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution was inactivated at 100°C and then centrifuged, and the liquid phase components were added with 2 times (V / V) ethanol to remove the polysaccharide and nucleic acid impurities.
[0030] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 8kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides.
[00...
Embodiment 3
[0033] (1) Extraction and enzymatic hydrolysis of yeast protein: mix dry yeast powder and water at a solid-to-liquid ratio of 1: 15 g / mL, and select neutral endopeptidase 30% wt, carboxypeptidase 30% wt and flavor Protease 40% wt mixed enzyme enzymatic hydrolysis. The amount of enzyme added was 2.5% wt of dry yeast powder, the enzymatic hydrolysis temperature was 20°C, and the enzymatic hydrolysis time was 4 hours.
[0034] (2) Separation and removal of impurities in the enzymatic hydrolysis solution: the enzymatic hydrolysis solution was inactivated at 110°C and then centrifuged, and the liquid phase components were added to 6 times (V / V) ethanol to remove the polysaccharide and nucleic acid impurities.
[0035] (3) Ultrafiltration membrane separation of active polypeptides: use ultrafiltration membranes with a molecular weight cut-off of 10kDa to filter and separate, and the filtrate is concentrated under reduced pressure and vacuum freeze-dried to obtain active polypeptides...
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