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168 results about "Exoenzyme" patented technology

An exoenzyme, or extracellular enzyme, is an enzyme that is secreted by a cell and functions outside of that cell. Exoenzymes are produced by both prokaryotic and eukaryotic cells and have been shown to be a crucial component of many biological processes. Most often these enzymes are involved in the breakdown of larger macromolecules. The breakdown of these larger macromolecules is critical for allowing their constituents to pass through the cell membrane and enter into the cell. For humans and other complex organisms, this process is best characterized by the digestive system which breaks down solid food via exoenzymes. The small molecules, generated by the exoenzyme activity, enter into cells and are utilized for various cellular functions. Bacteria and fungi also produce exoenzymes to digest nutrients in their environment, and these organisms can be used to conduct laboratory assays to identify the presence and function of such exoenzymes. Some pathogenic species also use exoenzymes as virulence factors to assist in the spread of these disease causing microorganisms. In addition to the integral roles in biological systems, different classes of microbial exoenzymes have been used by humans since pre-historic times for such diverse purposes as food production, biofuels, textile production and in the paper industry. Another important role that microbial exoenzymes serve is in the natural ecology and bioremediation of terrestrial and marine environments.

Fungal fibrinolytic enzyme and cultivating method thereof

The invention discloses a fungal fibrinolytic enzyme and a preparation method thereof. The fungal fibrinolytic enzyme is prepared by taking a Cordyceps militaris strain as a strain and performing liquid fermentation culture, separation and purification. The relative molecular weight of the fibrinolytic enzyme is about 21,000. The Cordyceps militaris fibrinolytic enzyme has good thrombolytic performance, is free from obvious acute toxicity, has prospects for clinical trial, development and application, and adds a new member to a rare fibrinolytic enzyme family. Particularly, a preferable fibrinolytic-enzyme Cordyceps militaris strain C.LSG-1 obtained through severe screening is rough in growth conditions, short in enzyme production cycle and capable of harvesting a large amount of Cordyceps militaris mycelium during enzyme production, and is the strain excellent in production performance. The Cordyceps militaris fibrinolytic enzyme is an extracellular enzyme which is particularly beneficial to subsequent separation and purification during preparation. The fungal fibrinolytic enzyme takes corn protein with extensive sources as a main culture medium, thereby having low cost for raw materials and providing a novel way for developing and utilizing the prior resources.
Owner:QIQIHAR UNIVERSITY

Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation

InactiveCN1884509AIncrease fermentation gas productionPromote degradationHydrolasesMicroorganismsBiotechnologyMethane yield
The method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation belongs to preparation of supplementary exoenzyme used for marsh gas fermentation. The invention is to provide a method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation, which has the production bacteria with the following enzymatic vitality index: selecting wheat bran and rice bran as mixed culture medium, obtaining mixed bran seeds by pedigree breed two-stage enlargement culture of the bacteria with solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic vitality index, adding commodity enzyme preparation to reach enzymatic vitality index, and airing until the humidity is lower than 5%. The enzymatic vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug tyrosine / g / min. The bacteria can be obtained by bacteria and fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the methane tank fermentation raw materials, the rate of dissolution and degradation of the marsh gas fermentation raw materials can be improve, and the gas-producing rate of marsh gas fermentation, TS and VS gas-production potential and methane content in marsh gas can be increased.
Owner:YUNNAN NORMAL UNIV

PelB signal peptide mutant capable of improving protein secretion efficiency and application of pelB signal peptide mutant

The invention discloses a pelB signal peptide mutant capable of improving the protein secretion efficiency and an application of the pelB signal peptide mutant and belongs to the technical field of gene engineering. A sequence of a pelB mutant secretive expression signal peptide is as shown in SEQ ID NO.1. The signal peptide is capable of improving the extracellular enzyme activity of objective protein cyclodextrin glycosyltransferase by 1.6 times. The extracellular protein production capability of recombinant escherichia coli transformed by using the signal peptide is strengthened, and industrialized production is facilitated.
Owner:JIANGNAN UNIV

Calcium alginate-sodium carboxymethyl cellulose co-immobilized Phanerochaete chrysosporium strain and biological microcapsule of exoenzyme thereof, and preparation method of strain

The invention relates to a calcium alginate-sodium carboxymethyl cellulose co-immobilized Phanerochaete chrysosporium strain and a biological microcapsule of exoenzyme thereof, and a preparation method of the strain. The biological microcapsule comprises a shell and a shell inside core, wherein the shell material is a calcium alginate gel, and a Phanerochaete chrysosporium exoenzyme crosslinking aggregate is embedded inside the calcium alginate gel; and the shell inside core is a calcium chloride-sodium carboxymethyl cellulose water solution, and Phanerochaete chrysosporium cells and an extracellular crosslinking enzyme aggregate are dispersed inside the calcium chloride-sodium carboxymethyl cellulose water solution. In the biological microcapsule preparation process, the Phanerochaete chrysosporium exoenzyme is directly prepared into the crosslinking enzyme aggregate, thereby omitting the step of purification of the lignin degrading enzyme, effectively lowering the production cost and enhancing the degradation efficiency since the crosslinking enzyme aggregate and Phanerochaete chrysosporium form a close relation.
Owner:山东济清科技服务有限公司

Method for efficiently synthesizing L-theanine through recombined corynebacterium crenatum

The invention discloses a method for efficiently synthesizing L-theanine through recombined corynebacterium crenatum, and belongs to the field of genetic engineering and enzyme engineering. According to the method, it is firstly verified that GGT signal peptide of a bacillus subtilis source can achieve secretory expression in a C.glutamicum system. Meanwhile, extracellular enzyme activity of recombinant bacteria C.glutamicum SDNN403/pXMJ19-tacM-ggt is about two times that of C.glutamicum SDNN403/pXMJ19-ggt, and it indicates that a tac Mpromoter is more beneficial for synthesis of GGT enzyme activity. Substrate flow and strategic high-yield L-theanine are adopted, a conversion system contains GGT with the concentration of 0.8 u/mL, the pH is 10, the temperature is 37 DEG C, and 22 mmol/L L-theanine and 66 mmol/L ethylamine are supplemented every other two hours from 0 h. The maximum theanine yield of 118 mmol is achieved when batch flow is added for 12 h, and the conversion rate is 92.8%. The secretory expression of gamma-glutamyltranspeptidase genes (ggt) of the B.subtilis source in C.glutamicum SDNN403 is achieved for the first time, and the highest yield of L-theanine reported currently and synthesized through recombined C.glutamicum is obtained by adding the substrate in batch flow.
Owner:JIANGNAN UNIV
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