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70 results about "Exoenzyme" patented technology

An exoenzyme, or extracellular enzyme, is an enzyme that is secreted by a cell and functions outside of that cell. Exoenzymes are produced by both prokaryotic and eukaryotic cells and have been shown to be a crucial component of many biological processes. Most often these enzymes are involved in the breakdown of larger macromolecules. The breakdown of these larger macromolecules is critical for allowing their constituents to pass through the cell membrane and enter into the cell. For humans and other complex organisms, this process is best characterized by the digestive system which breaks down solid food via exoenzymes. The small molecules, generated by the exoenzyme activity, enter into cells and are utilized for various cellular functions. Bacteria and fungi also produce exoenzymes to digest nutrients in their environment, and these organisms can be used to conduct laboratory assays to identify the presence and function of such exoenzymes. Some pathogenic species also use exoenzymes as virulence factors to assist in the spread of these disease causing microorganisms. In addition to the integral roles in biological systems, different classes of microbial exoenzymes have been used by humans since pre-historic times for such diverse purposes as food production, biofuels, textile production and in the paper industry. Another important role that microbial exoenzymes serve is in the natural ecology and bioremediation of terrestrial and marine environments.

Complex microbial inoculant for preventing and treating clubroot of rape

PendingCN120098849ABiocideFungiBiotechnologyAzotobacter chroococcum
The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.
Owner:INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI

Chitinase mutant as well as preparation method and application thereof

PendingCN120464606ABiocideBacteriaRandom mutationChitinase ChiA
The invention discloses a chitinase mutant obtained based on random mutation as well as a preparation method and application of the chitinase mutant. On the basis of an error-prone PCR (Polymerase Chain Reaction) technology, the mutant ChiA-7 (T165I / C253Y / A391T / N480S / T508S / K547T / I550V / N607T) of the chitinase ChiA of the bacillus thuringiensis is obtained by the invention. Compared with ChiA, the ChiA-7 has better stability, and the enzyme activity of the ChiA-7 is about 90% of the initial enzyme activity after the ChiA-7 is subjected to heat preservation for 3 hours at 40 DEG C in a phosphate buffer solution with the optimal catalysis condition of 40 DEG C and the pH value of 4.0, while the enzyme activity of the ChiA-7 is attenuated to 75% of the initial enzyme activity. The chiA-7 is connected with a carrier pET20b (+) and is transferred into escherichia coli Escherichia coli BL21 (DE3), and the extracellular enzyme activity of a recombinant bacterium reaches 10.23 U / mL, which is improved by 13.41% compared with the enzyme activity of a control bacterium for expressing chiA. The chitinase mutant obtained by the preparation method disclosed by the invention has good thermal stability and enzymatic activity, and can be applied to hydrolysis of chitin and inhibition of mould.
Owner:ZHENGZHOU UNIV

Red tricholoma matsutake culture medium using litchi branches as matrix and cultivation method thereof

The invention discloses a tricholoma matsutake culture medium using litchi branches as a matrix and a cultivation method thereof. The tricholoma matsutake culture medium is prepared from the following components in percentage by weight: 30-50% of litchi sawdust, 30-50% of cottonseed hulls, 3-8% of bran, 3-8% of corncobs, 3-8% of rice husks, 1-5% of corn flour and 1-5% of lime. The method is characterized in that the litchi branches are added into the stock liquid culture medium for making the branch seeds for the first time, on one hand, the tricholoma matsutake strains can be trained to generate extracellular enzymes capable of utilizing litchi wood chips, macromolecules of the litchi branches can be rapidly decomposed, and on the other hand, nutrient elements contained in the litchi branches can promote growth of tricholoma matsutake hyphae more rapidly, and the growth rate of the tricholoma matsutake hyphae is increased. The branch seeds are large in inoculation area and high in hybrid resistance, the growth time of mycelium of the cultivation material can be shortened, and the fruiting rate is increased. The invention not only provides a solution for innocent treatment of the litchi branches, but also finds a new culture medium for red tricholoma matsutake culture.
Owner:DONGGUAN AGRI SCI RES CENT

Application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.
Owner:SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +2

Sophora tonkinensis endophytic fungus talaromyces polymorphus ETXG-1-1-1 as well as culture method and application of talaromyces polymorphus ETXG-1-1-1

The invention discloses Talaromyces polyporus ETXG-1-1-1 as well as a culture method and application of the Talaromyces polyporus ETXG-1-1-1, the Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus ETXG-1-1-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 27, 2025, and the preservation number is CGMCC NO: 41927. The radix sophorae tonkinensis endophytic fungus talaromyces polyporus ETXG-1-1-1 is a talaromyces new species, can efficiently secrete extracellular enzymes such as cellulase and protease, can generate stable and high-color-gradation haematochrome, can effectively replace synthetic dyes and secondary metabolites to generate the effects of resisting bacteria, resisting oxidation, reducing blood sugar and the like, and can be used in the industries of textile, papermaking, food processing and the like.
Owner:JISHOU UNIVERSITY

Composite microbial agent for treating cyanobacterial bloom

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.
Owner:HUAZHONG UNIV OF SCI & TECH

Rhodopseudomonas telluris daeji-NZ23 strain having greenhouse gas reduction and plant growth-promoting activity and use thereof

The present invention relates to a (Rhodopseudomonas telluris DAEJI-NZ23 strain with accession number KACC81302BP, which exhibits nitrogen fixation capability, produces indole acetic acid (IAA), and has activities of reducing greenhouse gas, promoting plant growth, and secreting extracellular enzymes, and uses thereof. The strain of the present invention can be advantageously utilized in the technical field of greenhouse gas reduction, and is expected to increase crop yield while reducing excessive use of agricultural chemicals.
Owner:DAEJI DEV CO LTD +1

Preparation process of faint scent type floating beam black tea

The invention provides a preparation process of fen-flavor floating beam black tea, which comprises the following steps: constructing a multi-strain directional fermentation system consisting of debaryomyces hansenii, saccharomycopsis fibuligera and lactobacillus plantarum, and supplementing with seaweed kelose as a synergist; the problems of unstable quality and large flavor randomness caused by the fact that traditional black tea depends on natural microflora are solved. In the system, debaryomyces hansenii dominates to generate fresh and sweet floral compounds, saccharomycopsis fibuligera releases more aroma precursors by secreting extracellular enzyme and improves the mellowness of tea soup, and lactobacillus plantarum inhibits infectious microbes and optimizes the metabolic environment by producing acid; meanwhile, the seaweed kelose is used as an exclusive carbon source, so that rapid colonization and advantage establishment of functional flora are ensured. According to the technology, the faint scent type floating beam black tea with elegant and strong flower fragrance and fruit fragrance, fresh, refreshing and mellow taste and orange yellow and bright soup color can be finally and stably produced, and an effective path is provided for standardization and modernization upgrading of a traditional black tea technology.
Owner:浮梁县天祥茶号有限公司

Streptomyces flavus C-21 as well as preparation method and application of sterile fermentation filtrate of streptomyces flavus C-21

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.
Owner:SHANXI AGRI UNIV

Bactericidal composition containing tetramycin and Saisentong as well as preparation method and application of bactericidal composition

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.
Owner:ZHEJIANG DONGFENG CHEM IND CO LTD

Method for hydrolytic acidification of agricultural wastes and method for synthesizing medium-chain fatty acid

The invention provides an agricultural waste hydrolytic acidification method and a method for synthesizing medium-chain fatty acid, and belongs to the technical field of agricultural waste resource utilization. The invention provides an agricultural waste hydrolytic acidification method which comprises the following steps: after adjusting the solid content of agricultural waste, mixing the agricultural waste with short-chain AHLs and DPD-loaded chitosan microspheres to obtain a hydrolytic acidification system; and carrying out anaerobic fermentation on the hydrolytic acidification system to obtain a hydrolytic acidification product. According to the method, short-chain AHLs are added in the hydrolytic acidification process of the agricultural wastes to activate related genes of hydrolytic acidification bacteria, so that biofilm formation and extracellular enzyme secretion are promoted, the hydrolytic acidification efficiency of a substrate is improved, and substrate decomposition and short-chain fatty acid accumulation are promoted; dPD is released in a delayed manner in the metabolic peak period of acid-producing bacteria, the metabolic division of hydrolytic bacteria and acid-producing bacteria is coordinated, the activity of methanogens is inhibited, substrate competition is reduced, the degradation rate of agricultural waste cellulose is increased, and the yield of medium-chain fatty acid precursor substances such as acetic acid in fermentation substrates is increased.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Preparation process of a light-flavor floating beam black tea

The application provides a preparation process of a Qingxiang type floating beam black tea. A multi-strain directional fermentation system composed of Hansenula debayii, Kuraishia sp. and Lactobacillus plantarum and supplemented with trehalose as a synergist is constructed to improve the problems of unstable quality and great flavor randomness of traditional black tea caused by relying on natural microbial flora. In the system, Hansenula debayii dominates the generation of sweet and sweet floral compounds, Kuraishia sp. releases more aroma precursors by secreting extracellular enzymes and improves the thickness of tea soup, and Lactobacillus plantarum inhibits miscellaneous bacteria and optimizes the metabolic environment by producing acid; at the same time, trehalose as a special carbon source ensures the rapid colonization and advantage establishment of the functional flora. The process can finally stably produce Qingxiang type floating beam black tea with high and elegant floral and fruity aroma, fresh and mellow taste, and bright orange-yellow soup color, and provides an effective path for the standardization and modernization upgrade of traditional black tea process.
Owner:浮梁县天祥茶号有限公司

Method for promoting decomposition of straw returned to field

The present application belongs to the technical field of straw decomposition and soil improvement, and particularly relates to a method for promoting decomposition of straw returned to field. The present application forms a biological community of diazotrophs by applying artificial cultivation of nitrogen-fixing blue-green algae to a rice field, uses the diazotrophs to increase soil nitrogen by biological nitrogen fixation, and reduces the amount of nitrogen fertilizer applied. Meanwhile, the diazotrophs can also improve the activity of soil decomposing bacteria by secreting active substances such as extracellular enzymes, accelerate the conversion of straw source carbon to soil microbial source carbon, regulate the carbon-nitrogen balance of soil, and reduce the adverse effects on crops. On the other hand, the present application can reduce greenhouse gas emissions, and achieve rapid accumulation of soil organic matter and improvement of soil fertility.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI

Cyclocarya paliurus, golden flower and dark tea compound beverage and preparation method thereof

PendingCN121942785AHigh extraction rateHigh dissolution rateTea extractionFood shapingBiotechnologyCyclobalanopsis glauca
The invention discloses a cyclocarya paliurus eurotium cristatum dark tea compound beverage and a preparation method thereof. The method comprises the following steps: a biotransformation extraction step: crushing herbal raw materials to obtain mixed micro-powder, and carrying out co-fermentation enzymolysis on the mixed micro-powder and activated eurotium cristatum-containing golden flower dark tea powder in water to obtain a biotransformation herbal extracting solution; a coffee extraction step: performing low-temperature extraction and concentration on coffee beans to obtain a coffee concentrated solution; homogenizing and embedding, namely mixing the extracting solution, the concentrated solution and inulin, and performing high-pressure homogenizing treatment; and a drying and forming step: performing vacuum freeze drying on the homogenized feed liquid to obtain powder. The composition comprises the biotransformed herbal component prepared by the method, a coffee component and an inulin component. Herbal raw materials are biologically converted through an extracellular enzyme system of eurotium cristatum, the dissolution rate of functional components is increased, natural bitterness removal and sweetness increase are achieved, the product flavor is improved, and the biological activity is enhanced.
Owner:HUNAN XIANGTEA HI-TECH CD LTD

Double promoters for expressing sucrose phosphorylase and application of double promoters

PendingCN120574832AVectorsBacteriaDual promoterSucrose phosphorylase
The invention belongs to the field of gene engineering and the technical field of molecular biology, and particularly relates to double promoters for expressing sucrose phosphorylase and application of the double promoters. The double promoters comprise any one of the following sequences: a nucleotide sequence as shown in SEQ ID NO.1 or a complementary sequence of the nucleotide sequence; 1, and a nucleotide sequence as shown in SEQ ID NO. 2 or a complementary sequence thereof. The sucrose phosphorylase is synthesized in bacillus subtilis by adopting a double-promoter expression system, the effect of the sucrose phosphorylase is obviously superior to the expression effect of a single promoter P43, the extracellular enzyme activity is improved by 403%, and efficient production is realized. Meanwhile, the DNA fragment with the promoter and RBS functions provided by the invention can realize secretory expression of protein genes under the condition of not adding an inducer, provides an effective means for secretory expression of protein by bacillus subtilis by applying the DNA fragment to expression of sucrose phosphorylase, and has a very good development prospect.
Owner:ZHEJIANG UNIV OF TECH

Urate oxidase secretion signal peptide and use thereof

The application provides a uric acid oxidase secretion signal peptide and application thereof, and relates to the technical field of genetic engineering. In view of the problem that uric acid oxidases reported in the current literature are difficult to secrete in Pichia pastoris, the endogenous signal peptide of Pichia pastoris is screened, a signal peptide with strong secretion capacity is obtained, and the signal peptide is optimized in a targeted manner, a signal peptide mutant with strong secretion capacity is obtained, uric acid oxidase can be better secreted to the extracellular, and then the extracellular enzyme activity of uric acid oxidase is improved.
Owner:BLOOMATURE BIOTECHNOLOGY CO LTD

A method for increasing the production of lysozyme in European oysters

The present invention discloses a method for increasing the yield of European oyster lysozyme, and belongs to the field of bioengineering. The present invention screens and obtains European oyster-derived lysozyme with relatively high enzyme activity from 12 different sources of lysozyme gene sequences from vertebrates, mollusks, poultry and insects, and realizes the active expression of the enzyme in Pichia pastoris. The present invention also fuses short peptides and protein tags of different amino acids to the N-terminus of OElyz. When the Sumo tag is fused, the extracellular enzyme activity of recombinant OElyz reaches 382.6U / mL at the shake flask level, and this yield level can meet the application requirements of lysozyme in the fields of medicine, biotechnology and food.
Owner:AMPHASTAR NANJING PHARMA +1

A β-amylase-producing Bacillus subtilis strain and its application

The present invention discloses a strain of bacillus subtilis producing beta-amylase and its application, belong to the field of bioengineering technology, the bacillus subtilis is named as bacillus subtilis (Bacillus subtilis) JXJY-0811, has been deposited in the General Microbiology Center of China National Committee for the Collection of Microorganisms on June 7, 2024, and the deposit number is CGMCC NO.30886. The present invention uses the clear circle method to screen from soil and obtain bacterial strains that can secrete beta-amylase and amylase activity extracellularly, and is identified as bacillus subtilis through whole genome sequencing. The extracellular beta-amylase suitable temperature conditions of the strain are 50-75 DEG C, suitable pH value is 3.5-6.5, and extracellular enzyme activity is 9640.10U / mL, and after 60 hours of fermentation, the highest enzyme activity of 5L fermentation tank beta-amylase can reach 51980.36U / mL. The beta-amylase obtained by fermentation is used to process soluble starch solution, and the main product is maltose, which can be used for the production of maltose and high maltose syrup.
Owner:JIANGSU BOYANG BIOLOGICAL PROD CO LTD +1

Biological agent for sludge reduction and application thereof

The invention discloses a biological inoculant for sludge reduction and application thereof, the classification name of the inoculant is Stenotrophomonas sp. GY-25303, after high-density fermentation, sludge from different sources is treated, and the inoculant generates extracellular enzymes, degrades extracellular polymers (EPS), releases organic matters for denitrifying bacteria to use, and improves the removal rate of ammonia nitrogen. The production strain disclosed by the invention can grow in sludge environments of different sources, is high in extracellular enzyme yield and is good in sludge reduction effect; in the aspect of wastewater treatment, the effects of high COD (Chemical Oxygen Demand) removal rate, high ammonia nitrogen removal rate, high sludge reduction and the like are excellent; the nitrification and denitrification processes are not influenced while the sludge reduction is realized, and the sludge can be decomposed while the interference on functional flora is avoided, and the like. According to the present invention, the autonomously preserved patented strain with high extracellular enzyme yield is adopted, such that the sludge is significantly reduced from the biotechnology, the polluted water quality problem is significantly improved, and the broad market prospect is provided.
Owner:MAKING GREEN ENVIRONMENTAL TECH(SHANGHAI) CO LTD

Modified derived polypeptide and application thereof

The invention relates to a modified cellulase derived polypeptide and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The invention provides a derivative polypeptide of cellulose excision enzyme Cel7A, the cellulose excision enzyme Cel7A is derived from Trichoderma reesei, NCBI Acession No.XM006969162.1, and the amino acid sequence of the cellulose excision enzyme Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the amino acid sequence of the cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.
Owner:RONGCHENG HUIHAI CHUANGDA BIOTECHNOLOGY CO LTD

Preparation method of microbial fermentation five-flavor mannan medicated bath lotion

The invention provides a preparation method of a microbial fermentation five-flavor mannan medicated bath lotion, and belongs to the technical field of fermentation processes. The preparation method comprises the following steps: crushing Wuwei ganlu medicated bath raw materials, and carrying out wall breaking treatment through steam explosion to obtain a steam explosion substrate; adjusting the water content of the steam explosion substrate, inoculating enzyme-producing fungi to carry out aerobic solid-state fermentation, and carrying out enzymolysis on the substrate by utilizing an extracellular enzyme system secreted by the fungi to obtain a solid-state fermentation product; adding water into the solid-state fermentation product, inoculating transformed bacteria and saccharomycetes, carrying out anaerobic liquid-state fermentation, forming a water-alcohol-acid mixed system through microbial metabolism, and transforming and extracting active components to obtain fermented mash; and carrying out solid-liquid separation on the fermented mash, and carrying out membrane separation, filtration, sterilization and impurity removal on the obtained liquid to obtain the microbial fermentation five-flavor mannan medicated bath liquid. Through steam explosion physical modification and solid-liquid composite fermentation biotransformation, effective dissociation of medicinal material cell walls and improvement of substrate utilization rate are realized.
Owner:XUZHOU ARULA BIOTECHNOLOGY CO LTD

Method for hydrolyzing and acidifying agricultural waste and method for synthesizing medium-chain fatty acids

The present invention provides a method for hydrolyzing and acidifying agricultural waste and a method for synthesizing medium-chain fatty acids, belonging to the technical field of resource utilization of agricultural waste. The present invention provides a method for hydrolyzing and acidifying agricultural waste, comprising: adjusting the solid content of the agricultural waste, mixing it with short-chain AHLs and chitosan microspheres loaded with DPD to obtain a hydrolysis and acidification system; and subjecting the hydrolysis and acidification system to anaerobic fermentation to obtain a hydrolysis and acidification product. This method adds short-chain AHLs during the hydrolysis and acidification process of agricultural waste to activate genes related to hydrolysis and acidification bacteria, promote biofilm formation and extracellular enzyme secretion, improve substrate hydrolysis and acidification efficiency, and promote substrate decomposition and short-chain fatty acid accumulation; DPD is delayed in release during the metabolic peak of acid-producing bacteria, coordinating the metabolic division of hydrolytic bacteria and acid-producing bacteria, inhibiting the activity of methanogens, reducing substrate competition, improving the degradation rate of agricultural waste cellulose, and increasing the yield of medium-chain fatty acid precursors such as acetic acid in the fermentation substrate.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

An oil extraction system for kitchen waste and food waste

The present invention relates to the technical field of kitchen waste and food waste treatment, and provides a non-thermal oil extraction system for kitchen waste and food waste. According to the characteristics that vegetable oil in grease does not solidify, fat solidifies at low temperature, and there is a density difference between grease and water, the hydrolysis and acidification system liquefies the solid organic matter in the centrifuged residue through extracellular enzymes produced by hydrolysis microorganisms, destabilizes and stratifies the fat lacking organic matter nuclei, and then discharges the acidified liquid after hydrolysis and acidification into the secondary centrifugation to achieve the purpose of oil extraction. Microorganisms are used to stratify the grease, instead of the traditional method of heating to stratify the grease. This is the core of this patented invention. It reduces energy consumption and odor concentration, and achieves the purpose of energy conservation and consumption reduction while ensuring the oil extraction rate. Without heating throughout the process while ensuring the oil extraction rate, the composition and properties of the grease are not changed, which is beneficial to the further resource utilization of the grease.
Owner:SHENZHEN PANLONG ENVIRONMENTAL TECH CO LTD +1

An acetylacetone cleavage enzyme mutant and a method for producing 2-acetylcyclohexanone by an extracellular enzyme reaction

The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 2-acetylcyclohexanone through an extracellular enzyme reaction, and belongs to the technical field of biological engineering. In order to solve the technical problem of how to improve the yield of 2-acetylcyclohexanone generated through an extracellular enzyme reaction, the problem of limited Dke1 substrate types in a whole-cell synthesis system is solved, and the toxicity of 2-acetylcyclohexanone to cells is inhibited. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 105th position of the acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to alanine from glycine, and the yield of 2-acetylcyclohexanone generated through an enzyme reaction of the mutant in vitro reaches 14.22 mg / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Steel slag dephosphorizing method of compound microbial agent and application of steel slag dephosphorizing method

The invention relates to the technical field of solid waste treatment and recycling, and particularly discloses a steel slag dephosphorization method of a compound microbial agent and application of the steel slag dephosphorization method. The compound microbial agent comprises an aspergillus niger-burkholderia compound microbial agent, an improved NBRIP culture medium and a mixture of oxalic acid, citric acid, siderophore and extracellular enzyme, wherein the mixture is generated by metabolism of the aspergillus niger-burkholderia compound microbial agent; the preparation method comprises the following steps: firstly, inoculating the complex microbial inoculant into a culture medium, then carrying out shake culture in a dark place, carrying out centrifugation or suction filtration separation, and finally, collecting an active supernatant with the pH value of 2.0-3.5. The composition can be used for rapidly dephosphorizing the converter steel slag with the particle size of 0.075-0.5 mm at normal temperature, and has the advantages that the phosphorus dissolution rate is greater than or equal to 75%, no strong acid waste liquid is generated, the operation cost is low, and the method is green and environment-friendly; in addition, the preparation method disclosed by the invention has the advantages of simple process, normal temperature and normal pressure, continuous expanding culture of the microbial inoculum and reaction time of less than or equal to 30 minutes, and is further suitable for large-scale popularization.
Owner:SHANGHAI UNIV

Trichoderma reesei 5146-6, fungicide and application of Trichoderma reesei 5146-6 in preparation of biocontrol agent

The invention belongs to the technical field of active microorganisms, and particularly relates to Trichoderma reesei 5146-6, a microbial agent and application of the Trichoderma reesei 5146-6 in preparation of a biocontrol agent. The invention provides Trichoderma reesei 5146-6, which is proved to be capable of remarkably inhibiting the growth of phytopathogens such as rhizopus oryzae in the environment, metabolites of the Trichoderma reesei 5146-6 can inhibit various clinical pathogens, and broad-spectrum antibacterial active ingredients are expected to be further screened. In addition, the strain also has a plurality of extracellular enzyme secretion activities, including amylase and catalase, which are reported for the first time in the field.
Owner:LIAOCHENG UNIV

Method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through distributary seepage rotation irrigation

The invention discloses a method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through branched seepage rotation irrigation, and relates to the technical field of ecological restoration. According to the method, by differentiating branched seepage rotation irrigation modes, precisely matching habitat requirements of sexual and clone propagation populus euphratica seedlings, and adopting a long-interval and gradually-water-increasing irrigation mode for the clone propagation seedlings, the propagation of populus euphratica and the stability of rhizosphere microorganisms are regulated and controlled; according to the method, soil salt leaching and nitrogen and phosphorus accumulation can be promoted, functional florae such as rhizosphere actinomycetes and thick-wall bacteria are activated, complex organic matter is degraded by secreting extracellular enzyme, the phosphorus dissolving capacity is enhanced, the nutrient obtaining efficiency of seedlings in a medium-high-salt environment is improved, a short-interval and high-water-quantity mode is adopted for sexual propagation seedlings, a low-salt and high-humidity habitat is created, and the survival rate of the seedlings is increased. Growth-promoting florae such as pseudomonas are enriched, and by secreting growth hormones and antagonistic substances, the planting capacity of the seedlings in a humid environment is enhanced, so that directional regulation and control of the two breeding modes are realized.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI

Method for degrading ethyl carbamate in grape distilled liquor through functional microorganism co-fermentation

The invention relates to the technical field of fermentation processes, in particular to a method for degrading ethyl carbamate in grape distilled liquor through co-fermentation of functional microorganisms. Comprising the following steps: synchronously inoculating monascus purpureus and lactobacillus plantarum into grape mash; controlling an alcoholic fermentation stage; a trace element inducer is added into the wine liquid in a pulse mode, and extracellular enzyme secretion is induced; standing at 15 to 18 DEG C for 30 to 45 days; after distillation treatment, filling to obtain grape distilled liquor with low ethyl carbamate residue; monascus purpureus and lactobacillus plantarum are synchronously implanted for synergistic fermentation, so that the problem of low precursor degradation rate caused by single bacterium function limitation and acid inactivation in the existing microbiological method is solved; meanwhile, in cooperation with aging period pulse type microelement induction and oscillation activation, the problem that continuous conversion of a precursor cannot be blocked by a process inhibition method is solved; and finally, the cyanate precursor is deeply removed in the whole process, so that the residue of the distilled ethyl carbamate is reduced, and the safety standard is met.
Owner:XINJIANG TIANSHAN BINGHU WINE IND CO LTD

Decarbonization and nitrogen removal reactor for removing microbial metabolites from wastewater

The present invention provides a decarbonization and denitrification reactor for removing microbial metabolites in sewage, belonging to the field of sewage treatment. The reactor comprises an anaerobic microbial extracellular enzyme hydrolysis unit, an anaerobic decarbonization and denitrification filler layer unit, a secondary denitrification unit, an aerobic nitrification unit and an activated sludge separation unit which are sequentially connected, and the activated sludge separation unit is connected to the anaerobic microbial extracellular enzyme hydrolysis unit, so that when the nitrified liquid after aerobic treatment flows back to the anaerobic treatment part, the microbial metabolites can be removed, thereby enhancing the overall organic matter removal effect of the process.
Owner:TONGJI UNIV

Preparation method of microbial carrier as well as introduction and domestication method and application of microbial carrier

The invention discloses a preparation method of a microbial carrier, a microbial carrier introduction and domestication method and application, and the preparation method of the microbial carrier comprises the following steps: sequentially carrying out alkali treatment, washing, acid treatment and washing on a pine sawdust raw material to obtain neutral degreased pine sawdust; and drying the degreased pine sawdust to obtain the microbial carrier. The microbial carrier is applied to sewage treatment, sewage treatment is simulated, culture is performed for a period of time, microorganisms adsorbed on pine sawdust are gradually proliferated, extracellular enzyme slowly wraps the pine sawdust, active sawdust is formed, the active sawdust can serve as a microbial denitrification carbon source, the carbon source adding cost is saved, and the microbial denitrification effect is enhanced; the total nitrogen removal efficiency is relatively high. Meanwhile, the active sawdust is not easy to disintegrate, has a'mass center ', is large in specific gravity and high in precipitation speed, the precipitation speed is more than two times that of active sludge with the same sludge concentration, the solid-liquid separation effect is good, the sewage proportion in a treatment tank is favorably increased, and the productivity is improved.
Owner:遂宁国润排水有限公司