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35 results about "Exoenzyme" patented technology

An exoenzyme, or extracellular enzyme, is an enzyme that is secreted by a cell and functions outside of that cell. Exoenzymes are produced by both prokaryotic and eukaryotic cells and have been shown to be a crucial component of many biological processes. Most often these enzymes are involved in the breakdown of larger macromolecules. The breakdown of these larger macromolecules is critical for allowing their constituents to pass through the cell membrane and enter into the cell. For humans and other complex organisms, this process is best characterized by the digestive system which breaks down solid food via exoenzymes. The small molecules, generated by the exoenzyme activity, enter into cells and are utilized for various cellular functions. Bacteria and fungi also produce exoenzymes to digest nutrients in their environment, and these organisms can be used to conduct laboratory assays to identify the presence and function of such exoenzymes. Some pathogenic species also use exoenzymes as virulence factors to assist in the spread of these disease causing microorganisms. In addition to the integral roles in biological systems, different classes of microbial exoenzymes have been used by humans since pre-historic times for such diverse purposes as food production, biofuels, textile production and in the paper industry. Another important role that microbial exoenzymes serve is in the natural ecology and bioremediation of terrestrial and marine environments.

Application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism

PendingCN121271760ABiocidePlant growth regulatorsBiotechnologyPhytophthora sp.
The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.
Owner:SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +2

Rhodopseudomonas telluris daeji-NZ23 strain having greenhouse gas reduction and plant growth-promoting activity and use thereof

The present invention relates to a (Rhodopseudomonas telluris DAEJI-NZ23 strain with accession number KACC81302BP, which exhibits nitrogen fixation capability, produces indole acetic acid (IAA), and has activities of reducing greenhouse gas, promoting plant growth, and secreting extracellular enzymes, and uses thereof. The strain of the present invention can be advantageously utilized in the technical field of greenhouse gas reduction, and is expected to increase crop yield while reducing excessive use of agricultural chemicals.
Owner:DAEJI DEV CO LTD +1

Preparation process of faint scent type floating beam black tea

The invention provides a preparation process of fen-flavor floating beam black tea, which comprises the following steps: constructing a multi-strain directional fermentation system consisting of debaryomyces hansenii, saccharomycopsis fibuligera and lactobacillus plantarum, and supplementing with seaweed kelose as a synergist; the problems of unstable quality and large flavor randomness caused by the fact that traditional black tea depends on natural microflora are solved. In the system, debaryomyces hansenii dominates to generate fresh and sweet floral compounds, saccharomycopsis fibuligera releases more aroma precursors by secreting extracellular enzyme and improves the mellowness of tea soup, and lactobacillus plantarum inhibits infectious microbes and optimizes the metabolic environment by producing acid; meanwhile, the seaweed kelose is used as an exclusive carbon source, so that rapid colonization and advantage establishment of functional flora are ensured. According to the technology, the faint scent type floating beam black tea with elegant and strong flower fragrance and fruit fragrance, fresh, refreshing and mellow taste and orange yellow and bright soup color can be finally and stably produced, and an effective path is provided for standardization and modernization upgrading of a traditional black tea technology.
Owner:浮梁县天祥茶号有限公司

Streptomyces flavus C-21 as well as preparation method and application of sterile fermentation filtrate of streptomyces flavus C-21

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.
Owner:SHANXI AGRI UNIV

Bactericidal composition containing tetramycin and Saisentong as well as preparation method and application of bactericidal composition

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.
Owner:ZHEJIANG DONGFENG CHEM IND CO LTD

Preparation process of a light-flavor floating beam black tea

The application provides a preparation process of a Qingxiang type floating beam black tea. A multi-strain directional fermentation system composed of Hansenula debayii, Kuraishia sp. and Lactobacillus plantarum and supplemented with trehalose as a synergist is constructed to improve the problems of unstable quality and great flavor randomness of traditional black tea caused by relying on natural microbial flora. In the system, Hansenula debayii dominates the generation of sweet and sweet floral compounds, Kuraishia sp. releases more aroma precursors by secreting extracellular enzymes and improves the thickness of tea soup, and Lactobacillus plantarum inhibits miscellaneous bacteria and optimizes the metabolic environment by producing acid; at the same time, trehalose as a special carbon source ensures the rapid colonization and advantage establishment of the functional flora. The process can finally stably produce Qingxiang type floating beam black tea with high and elegant floral and fruity aroma, fresh and mellow taste, and bright orange-yellow soup color, and provides an effective path for the standardization and modernization upgrade of traditional black tea process.
Owner:浮梁县天祥茶号有限公司

Cyclocarya paliurus, golden flower and dark tea compound beverage and preparation method thereof

PendingCN121942785AHigh extraction rateHigh dissolution rateTea extractionFood shapingBiotechnologyCyclobalanopsis glauca
The invention discloses a cyclocarya paliurus eurotium cristatum dark tea compound beverage and a preparation method thereof. The method comprises the following steps: a biotransformation extraction step: crushing herbal raw materials to obtain mixed micro-powder, and carrying out co-fermentation enzymolysis on the mixed micro-powder and activated eurotium cristatum-containing golden flower dark tea powder in water to obtain a biotransformation herbal extracting solution; a coffee extraction step: performing low-temperature extraction and concentration on coffee beans to obtain a coffee concentrated solution; homogenizing and embedding, namely mixing the extracting solution, the concentrated solution and inulin, and performing high-pressure homogenizing treatment; and a drying and forming step: performing vacuum freeze drying on the homogenized feed liquid to obtain powder. The composition comprises the biotransformed herbal component prepared by the method, a coffee component and an inulin component. Herbal raw materials are biologically converted through an extracellular enzyme system of eurotium cristatum, the dissolution rate of functional components is increased, natural bitterness removal and sweetness increase are achieved, the product flavor is improved, and the biological activity is enhanced.
Owner:HUNAN XIANGTEA HI-TECH CD LTD

Urate oxidase secretion signal peptide and use thereof

The application provides a uric acid oxidase secretion signal peptide and application thereof, and relates to the technical field of genetic engineering. In view of the problem that uric acid oxidases reported in the current literature are difficult to secrete in Pichia pastoris, the endogenous signal peptide of Pichia pastoris is screened, a signal peptide with strong secretion capacity is obtained, and the signal peptide is optimized in a targeted manner, a signal peptide mutant with strong secretion capacity is obtained, uric acid oxidase can be better secreted to the extracellular, and then the extracellular enzyme activity of uric acid oxidase is improved.
Owner:BLOOMATURE BIOTECHNOLOGY CO LTD

Modified derived polypeptide and application thereof

The invention relates to a modified cellulase derived polypeptide and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The invention provides a derivative polypeptide of cellulose excision enzyme Cel7A, the cellulose excision enzyme Cel7A is derived from Trichoderma reesei, NCBI Acession No.XM006969162.1, and the amino acid sequence of the cellulose excision enzyme Cel7A is as shown in SEQ ID NO: 1. The invention further provides a preparation method of the derivative polypeptide of the cellulose excision enzyme Cel7A. Wherein the amino acid sequence of the cellulose binding domain CBM is as shown in SEQ ID NO: 2. The derivative polypeptide is a polypeptide of which the amino acid sequence is 70% or more identical to that of a polypeptide shown in SEQ ID NO: 2, can reduce combination with lignin and improve the degradation efficiency of cellulose, so that the saccharification efficiency of cellulase is improved, the post-treatment process cost is greatly saved, and the derivative polypeptide is suitable for industrial production. The method has important industrial application value.
Owner:RONGCHENG HUIHAI CHUANGDA BIOTECHNOLOGY CO LTD

Preparation method of microbial fermentation five-flavor mannan medicated bath lotion

The invention provides a preparation method of a microbial fermentation five-flavor mannan medicated bath lotion, and belongs to the technical field of fermentation processes. The preparation method comprises the following steps: crushing Wuwei ganlu medicated bath raw materials, and carrying out wall breaking treatment through steam explosion to obtain a steam explosion substrate; adjusting the water content of the steam explosion substrate, inoculating enzyme-producing fungi to carry out aerobic solid-state fermentation, and carrying out enzymolysis on the substrate by utilizing an extracellular enzyme system secreted by the fungi to obtain a solid-state fermentation product; adding water into the solid-state fermentation product, inoculating transformed bacteria and saccharomycetes, carrying out anaerobic liquid-state fermentation, forming a water-alcohol-acid mixed system through microbial metabolism, and transforming and extracting active components to obtain fermented mash; and carrying out solid-liquid separation on the fermented mash, and carrying out membrane separation, filtration, sterilization and impurity removal on the obtained liquid to obtain the microbial fermentation five-flavor mannan medicated bath liquid. Through steam explosion physical modification and solid-liquid composite fermentation biotransformation, effective dissociation of medicinal material cell walls and improvement of substrate utilization rate are realized.
Owner:XUZHOU ARULA BIOTECHNOLOGY CO LTD

An acetylacetone cleavage enzyme mutant and a method for producing 2-acetylcyclohexanone by an extracellular enzyme reaction

The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 2-acetylcyclohexanone through an extracellular enzyme reaction, and belongs to the technical field of biological engineering. In order to solve the technical problem of how to improve the yield of 2-acetylcyclohexanone generated through an extracellular enzyme reaction, the problem of limited Dke1 substrate types in a whole-cell synthesis system is solved, and the toxicity of 2-acetylcyclohexanone to cells is inhibited. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 105th position of the acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to alanine from glycine, and the yield of 2-acetylcyclohexanone generated through an enzyme reaction of the mutant in vitro reaches 14.22 mg / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through distributary seepage rotation irrigation

The invention discloses a method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through branched seepage rotation irrigation, and relates to the technical field of ecological restoration. According to the method, by differentiating branched seepage rotation irrigation modes, precisely matching habitat requirements of sexual and clone propagation populus euphratica seedlings, and adopting a long-interval and gradually-water-increasing irrigation mode for the clone propagation seedlings, the propagation of populus euphratica and the stability of rhizosphere microorganisms are regulated and controlled; according to the method, soil salt leaching and nitrogen and phosphorus accumulation can be promoted, functional florae such as rhizosphere actinomycetes and thick-wall bacteria are activated, complex organic matter is degraded by secreting extracellular enzyme, the phosphorus dissolving capacity is enhanced, the nutrient obtaining efficiency of seedlings in a medium-high-salt environment is improved, a short-interval and high-water-quantity mode is adopted for sexual propagation seedlings, a low-salt and high-humidity habitat is created, and the survival rate of the seedlings is increased. Growth-promoting florae such as pseudomonas are enriched, and by secreting growth hormones and antagonistic substances, the planting capacity of the seedlings in a humid environment is enhanced, so that directional regulation and control of the two breeding modes are realized.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI

Method for determining and generating prescription of rhizosphere microbial nutrient limitation based on extracellular enzyme activity

PendingCN122259844AEarth material testingMicroorganismSoil ecology
The application discloses a rhizosphere microbial nutrient limitation judgment and prescription generation method based on extracellular enzyme activity, and belongs to the technical field of soil ecology and agricultural detection and analysis. The method obtains soil samples of a plough layer and a sub-plough layer by stratified discrete sampling of a to-be-tested farmland soil, determines extracellular enzyme activity data of each soil sample, and obtains a microbial nutrient limitation strength by processing the enzyme measurement characteristic ratio based on the extracellular enzyme activity data through a threshold ecological enzyme measurement model. The microbial nutrient limitation type is judged and the microbial nutrient limitation strength difference is calculated based on the microbial nutrient limitation strength of each soil sample, so as to form a soil microbial nutrient limitation pattern of the plough layer and the sub-plough layer, and a differentiated nutrient management prescription of a corresponding layer is output according to the soil microbial nutrient limitation pattern. The application realizes accurate quantitative judgment of rhizosphere microbial carbon, nitrogen and phosphorus nutrient limitation, provides a scientific basis for stratified accurate management of farmland nutrients, and improves the pertinence and effectiveness of nutrient regulation.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

Bacillus subtilis genetic engineering strain for high yield of 4-alpha-glycosyltransferase, and construction method and application thereof

The invention discloses a bacillus subtilis genetic engineering strain of high-yield 4-alpha-glycosyltransferase (4GT) as well as a construction method and application of the bacillus subtilis genetic engineering strain. The engineering bacterial strain is constructed by taking bacillus subtilis DB403 as a host and introducing a recombinant plasmid pHTN13 / PspovG-PspovG-SPsacB-4GT, and the engineering bacterial strain is constructed by taking bacillus subtilis DB403 as the host. According to the invention, the powerful constitutive tandem promoter PspovG-PspovG is combined with the efficient signal peptide SPsacB, and is combined with the 4GT gene, so that the secretory expression efficiency of 4GT in bacillus subtilis is remarkably improved. According to the engineering strain, under the optimized fermentation condition, the extracellular 4GT enzyme activity of shake flask fermentation can reach 88.53 U / mL and is improved by 96.21% compared with that of an original strain. The constructed engineering strain can be used for producing the 4GT enzyme preparation with low cost and high efficiency, and has important application value in the fields of starch modification, functional food development and the like.
Owner:EAST CHINA NORMAL UNIV +1

Microorganism for treating blue-green algae and stink in park landscape lake water and culture process thereof

PendingCN121605981ABiocideBacteriaBiotechnologyOdor source
The invention discloses a microorganism for treating cyanobacteria and stink in park landscape lake water and a culture process thereof, the microorganism comprises a microbial agent, a compound enzyme, tartaric acid and free fatty acid, and the microbial agent is composed of biphasic photosynthetic bacteria, bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium. The microbial agent is compounded with the compound enzyme, the tartaric acid and the free fatty acid, five strains in the microbial agent are clear in division of labor, the biphasic photosynthetic bacteria decompose organic matters released by cyanobacteria residues and reduce ammonia nitrogen, the bifidobacterium adjusts the balance of water flora and inhibits reproduction of malodorous bacteria, and the mesophilic actinomycetes secretes antibacterial substances to prevent recurrence of the cyanobacteria. Nitrosobacteria convert an odor source into harmless substances, bacillus megaterium further secretes extracellular enzyme to accelerate degradation of residues, and microorganisms and bioactive substances have a synergistic effect, so that the removal period of blue-green algae is shortened, and the odor removal rate is increased and is far superior to that of a single microbial preparation.
Owner:HEYUAN SUNSHINE BIOTECHNOLOGY CO LTD

Ectonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) inhibitors for the treatment of hypophosphatasia

A method for treating hypophosphatasia (HPP) is provided herein, comprising administering a therapeutically effective dose of an ectonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) inhibitor. A method for using an ENPP1 inhibitor to treat or prevent cartilage disease is disclosed herein. In some embodiments, the cartilage disease is hypophosphatasia. Ectonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) is an extracellular enzyme that hydrolyzes ATP to PPi and AMP in the extracellular space.
Owner:1 SEABIO INC

An acetylacetone cleavage enzyme mutant and a method for producing 3-methyl-2,4-pentanedione by an extracellular enzyme reaction

The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 3-methyl-2,4-pentanedione by extracellular enzyme reaction, and belongs to the technical field of biological engineering. The application solves the problems of Dke1 substrate type inhibition in a whole cell synthesis system and the problem of 3-methyl-2,4-pentanedione toxicity inhibition on cells, and aims to improve the yield of 3-methyl-2,4-pentanedione. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 117th position of acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to glycine from methionine, and the amino acid sequence of the mutant is shown in SEQ ID NO. 3. The acetylacetone cleavage enzyme mutant can convert 2,3-butanedione and acetic acid into 3-methyl-2,4-pentanedione by enzyme reaction in vitro, and the yield reaches 23.29 mg / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Soil carbon immobilization improver based on agricultural waste compost humification as well as preparation method and application of soil carbon immobilization improver

The invention belongs to the technical field of soil amendments, and particularly relates to a soil carbon immobilization amendment based on agricultural waste compost humification and a preparation method and application of the soil carbon immobilization amendment. The preparation method comprises the following steps: mixing the rotten vegetable leaves with straw powder for aerobic composting, and mixing the obtained fermentation product with glutamine for humification to obtain a decomposed material; mixing the decomposed material, modified charcoal and polyglutamic acid to obtain a mixed base material; the modified biochar is obtained by pyrolyzing rotten vegetable leaves in an inert atmosphere, activating with phosphoric acid and then performing secondary pyrolysis; and mixing the mixed base material and the compound functional bacterial liquid, and carrying out micro-aerobic directional after-ripening to obtain the soil carbon immobilization improver. According to the invention, glutamine is added to enhance the synthesis of humic acid, the modified charcoal has a mesoporous structure and can physically isolate microbial extracellular enzyme, so that the biodegradation half-life period of the loaded humic acid is prolonged, carboxyl of polyglutamic acid and phenolic hydroxyl of humic acid form a hydrogen bond network, the stability of an aggregate can be enhanced, and efficient and lasting carbon sequestration is realized.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Intervention system for Chinese yam tuber cells based on different soil environments

The invention discloses an intervention system for Chinese yam tuber cells based on different soil environments, and particularly relates to the technical field of Chinese yam planting intervention. A structural organic fiber material and an organic precursor material are selected to prepare a physical support carrier; a metabolic active matrix rich in extracellular enzyme systems and biostimulants is obtained; constructing a directional induction intervention layer in the underground tuber expansion area, and arranging a buffer layer and a transition structure for maintaining waterpower continuity above the directional induction intervention layer; new tubers are guided to intrude into the intervention layer, and a direct physical contact interface between plant tissues and matrix hyphae is established; in a tuber expansion period, inducing signal accumulation under micro-stress by utilizing pulse type oscillation regulation of matrix water potential, and directionally pumping precursor substances and signal molecules into cortical cells by utilizing plant transpiration pulling force in a rehydration period; the problems that in Chinese yam planting, due to the fact that microcosmic induction signals and transmission power are lacked, mucus cell differentiation is blocked, and the internal quality is low are effectively solved.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Method for producing humus by utilizing gramineous plant waste in cooperation with organic solid waste

The invention discloses a method for producing humus by using gramineous plant waste and organic solid waste, which comprises the following steps: raw material pretreatment: crushing the gramineous plant waste into a first particle material, and carrying out sorting, impurity removal, dehydration, deoiling and desalination treatment on the organic solid waste rich in nitrogen source to obtain a second particle material; premixing and compatibility of microbial agents: adding a specific efficient compound microbial agent compounded by a microbial living body and exoenzyme secreted by the microbial living body into the first particle material, then mixing with the second particle material according to a dry matter mass ratio of (1-5): 1, and adjusting the initial C / N to be (20-30): 1 and the water content to be 50-70%; performing aerobic composting fermentation, controlling the temperature of a high-temperature stage to be 55-70 DEG C, maintaining the temperature for 5-10 days, and keeping the total period for 25-35 days; after-ripening and screening, aging for 5-10 days after fermentation, and screening to obtain a humus product. According to the method disclosed by the invention, the specific microbial agent and the multi-source material cooperate to efficiently degrade lignocellulose and directionally convert organic carbon into humus, so that the composting period is remarkably shortened, and the carbon preservation rate and the product quality are improved.
Owner:BESTA (HANGZHOU) BIOCYCLE TECHNOLOGY CO LTD +3

Metabolic adaptive coordinated control method for solid state fermentation of aspergillus oryzae

PendingCN122629168AData setNetwork model
This invention discloses a metabolic adaptive and collaborative control method for Aspergillus oryzae solid-state fermentation, belonging to the field of microbial fermentation regulation technology. It includes collecting fermentation state datasets from mycelial morphology descriptions at multiple fermentation sampling times, near-infrared spectral time-series data of the culture medium, and extracellular enzyme activity data. Semantic feature vectors are extracted from the mycelial morphology text, and spectral fluctuation feature vectors are obtained by dynamic peak tracking of the near-infrared spectral time-series data. These are then used to generate a fused fermentation feature set through cross-modal feature alignment. A pre-trained graph convolutional network model is used to complete the metabolic regulation weight allocation, outputting the correlation distribution between extracellular enzyme activity indicators and each metabolic pathway. Combined with the correlation distribution, joint metabolic source tracing is performed to generate metabolic adaptive regulation decisions at each sampling time. This method can achieve deep fusion of multi-source heterogeneous fermentation data, accurately characterize fermentation metabolic relationships, and adapt to the dynamic regulation needs of Aspergillus oryzae solid-state fermentation.
Owner:SHANDONG HEZHONG KANGYUAN BIOLOGY SCI & TECH CO LTD

DNA (Deoxyribose Nucleic Acid) fragment with promoter function of bacillus subtilis and application of DNA fragment

PendingCN121991957Agood effectIncreased extracellular enzyme activityBacteriaTransferasesSucrose phosphorylasePhosphorylation
The invention belongs to the field of genetic engineering and the technical field of molecular biology, and relates to a DNA fragment, in particular to a DNA fragment, with a promoter function, of bacillus subtilis and application of the DNA fragment in production of sucrose phosphorylase. According to the method for screening the single promoter, efficient secretory expression of BbrSP-VF and BadSP-QL is achieved in bacillus subtilis, the effect of the method is remarkably superior to the expression effect of a single promoter P43, and the extracellular enzyme activity is improved by 247% and 236% respectively. According to the DNA fragment with the promoter, secretory expression of protein genes can be achieved under the condition that an inducer does not need to be added, the DNA fragment with the promoter is applied to expression of sucrose phosphorylase, an effective means is provided for secretory expression of protein by bacillus subtilis, and the DNA fragment with the promoter has a good development prospect.
Owner:ZHEJIANG UNIV OF TECH

Application of chondroitin sulfate exosidase mutant and its enzymolysis product in treatment of alcoholic liver injury

PendingCN122445620ANucleotideAmino acid
The application relates to application of a chondroitin sulfate exoenzyme mutant and an enzymolysis product thereof to treatment of alcoholic liver injury, and belongs to the field of enzyme engineering.The amino acid sequence of the mutant is shown in SEQ ID NO. 1.The nucleotide sequence for coding the amino acid sequence shown in SEQ ID NO. 1 is shown in SEQ ID NO. 2, the gene fragment of the nucleotide sequence is obtained through PCR amplification and sequencing on the basis of chondroitin sulfate exoenzyme of the original amino acid sequence SEQ ID NO. 3, and the mutant is obtained through an error-prone PCR reaction on the gene fragment.The application also provides a recombinant expression vector containing the mutant gene and a recombinant bacterium.The mutant, the recombinant expression vector and the recombinant bacterium can be used to prepare low molecular weight chondroitin sulfate by taking cartilage as raw material.The low molecular weight chondroitin sulfate has an auxiliary protection function for alcoholic liver injury.
Owner:OCEAN UNIV OF CHINA

Quorum sensing inhibitor and application thereof in prevention and treatment of pear fire disease pathogenic bacteria

The invention discloses a quorum sensing inhibitor and application of the quorum sensing inhibitor in prevention and treatment of pear fire disease pathogenic bacteria, the effective component of the quorum sensing inhibitor is 9 amino-1, 2, 3, 4,-tetrahydroacridine, by applying the quorum sensing inhibitor provided by the invention, the inhibition rate of biofilm generation of the pear fire disease pathogenic bacteria can reach 93.4%, and the inhibition rate of exopolysaccharide formation can reach 66.4%; the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of pear fire disease pathogenic bacteria, and the activity of extracellular enzymes related to pathogenic bacteria infection, including protease, cellulase, xylanase, pectate lyase and polygalacturonase, is obviously reduced, so that the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of the pear fire disease pathogenic bacteria, and can be used for preparing the quorum sensing inhibitor for pear fire disease pathogenic bacteria by reducing expression of virulence factors of the quorum sensing inhibitor. Therefore, the infection process is delayed, the infectivity of pear fire blight is reduced, and the damage to plants is reduced. According to the technical scheme provided by the invention, an innovative path from'pathogenic mechanism analysis' to'ecological adaptability intervention 'is opened up for comprehensive prevention and control of pear fire blight.
Owner:XINJIANG ACAD OF AGRI SCI (XINJIANG BRANCH OF CHINESE ACAD OF AGRI SCI)

Stenotrophomonas maltophilia capable of degrading lignin and resisting bacteria and application of stenotrophomonas maltophilia

The invention discloses stenotrophomonas maltophilia with lignin degradation and antibacterial effects and application of the stenotrophomonas maltophilia, and relates to the field of microorganisms. The stenotrophomonas maltophilia mjt3 is preserved in the China General Microbiological Culture Collection Center on January 20, 2026, the preservation date is January 20, 2026, and the preservation number is CGMCC No.37470. The stenotrophomonas maltophilia mjt3 can be used for preparing a stenotrophomonas maltophilia strain. The stenotrophomonas maltophilia mjt3 disclosed by the invention is a multifunctional biocontrol strain with both lignin degradation capability and pathogenic bacterium inhibition activity. The strain inhibits the growth of pathogenic bacteria through a plurality of mechanisms such as antibacterial substance generation, ecological niche competition and extracellular enzyme secretion, degrades agricultural wastes, not only can effectively control the infection of pathogenic bacteria such as alternaria alternata, but also can obviously reduce the use of chemical pesticides, improve the soil micro-ecology and improve the soil quality. And an eco-friendly solution is provided for realizing green prevention and control of diseases, cyclic utilization of resources and environmental protection.
Owner:TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (QINGZHOU TOBACCO RESEARCH INSTITUTE OF CHINA NATIONAL TOBACCO COMPANY)

Composite material with toxin-producing fungus resisting activity and fungaltoxin degrading effect

PendingCN121444916ABiocideEdible seed preservationAspergillus ochraceopetaliformisCorn meal
The invention provides a composite material with both toxin-producing fungus resisting activity and fungaltoxin degrading effect. According to the invention, montmorillonite is taken as a carrier, and the advantages of bacillus, trichoderma reesei extracellular enzyme, cinnamyl aldehyde and montmorillonite are combined and compounded into a composite material with both toxin-producing fungus activity resistance and mycotoxin degradation effect, so that the composite material has both toxin-producing fungus inhibition and mycotoxin degradation effects; the compound can be applied to feed and grain products to inhibit mildew and reduce mycotoxin pollution. When the composite material is added into the corn flour polluted by toxin-producing bacteria and mycotoxin, the composite material can significantly inhibit the growth of toxin-producing fungi (fusarium graminearum, fusarium equiseti, aspergillus flavus and aspergillus ochraceus) in the corn flour, and the AFB1 pollution inhibition rate and the ZEN pollution inhibition rate are 98.89% and 93.54% respectively.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES

Fibrinolytic Pseudomonas spp. QMS71 from mangrove wetlands and its application

PendingCN122303083ABiotechnologyPseudomonas
This invention discloses a fibrinolytic enzyme-producing Pseudomonas hygroscopicus originating from mangrove wetlands (… Fictibacillus rigui The strain QMS71 is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 37018. Under suitable culture conditions, strain QMS71 can produce extracellular enzymes with fibrin-degrading activity. Its fermentation products exhibit significant fibrin degradation activity in vitro and demonstrate thrombolytic and anticoagulant activities in relevant in vitro functional evaluation models. This invention provides a well-identified fibrinolytic enzyme-producing microbial resource and its applications, which can be used for in vitro functional evaluation, model studies, and functional enzymology research related to fibrin degradation.
Owner:JIANGSU OCEAN UNIV