7 results about "Cross-linked enzyme aggregate" patented technology

The invention provides a polydopamine cross-linked lipase aggregate and a preparation method thereof, and relates to the technical field of enzyme immobilization. The polydopamine cross-linked lipase aggregate is mainly prepared from lipase and a dopamine hydrochloride cross-linking agent through a Schiff base and Michael addition reaction and a cross-linked enzyme aggregate technology. The preparation method of the polydopamine cross-linked lipase aggregate is simple, convenient and controllable; the prepared polydopamine cross-linked lipase aggregate still has excellent enzyme activity in a heavy metal salt solution.

The application discloses a kind of β-lactamase immobilized sponge and its preparation method and application, belong to environmental engineering technical field, the present application utilizes polydopamine coated melamine sponge as carrier, by crosslinking method β-lactamase crosslinking enzyme aggregate is fixed on the surface of the carrier, obtain β-lactamase immobilized sponge (MS@β-lactamase-CLEAs), to remove antibiotic in waste water efficiently.The stability and catalytic activity of β-lactamase are enhanced by the method of the application, the MS@β-lactamase-CLEAs of the application can quickly and efficiently hydrolyze β-lactam antibiotics in waste water, significantly reduce its concentration;Compared with traditional treatment method, the present application has higher processing efficiency and good reusability, provides a kind of feasible solution for the treatment of antibiotic pollution wastewater, has wide application prospect.

The invention provides a preparation method and a use method of a microchannel immobilized cross-linked enzyme aggregate (CLEAs) reactor. Comprising the following steps: S1, preparing an enzyme solution; s2, preparation of a precipitant-cross-linking agent mixed solution: mixing a precipitant acetone and a cross-linking agent glycerol diglycidyl ether in proportion to obtain the precipitant-cross-linking agent mixed solution; s3, preparation of a micro-channel reactor: drawing an AUTO CAD drawing of the micro-channel reactor, inputting the AUTO CAD drawing into a laser engraving machine, and obtaining the micro-channel reactor after engraving is completed; s4, preparation of a microchannel immobilized CLEAs reactor: firstly, carrying out surface modification on the reactor by using PEI, then simultaneously injecting an enzyme solution and a precipitant-cross-linking agent mixed solution into the microchannel through a Y-shaped tube, carrying out enzyme protein molecule precipitation and cross-linking to form CLEAs, and immobilizing the CLEAs on the surface of the microchannel wall through the cross-linking action of a cross-linking agent and the adsorption action of the surface of the microchannel to obtain the microchannel immobilized CLEAs reactor. And thus, the micro-channel immobilized CLEAs reactor is obtained. According to the invention, efficient and continuous use of the micro-channel reactor and CLEAs is realized, and the method is suitable for industrial production.

The invention discloses a beta-lactamase immobilized sponge and a preparation method and application thereof, and belongs to the technical field of environmental engineering.Polydopamine coated melamine sponge is used as a carrier, beta-lactamase cross-linked enzyme aggregate is immobilized on the surface of the carrier through a cross-linking method, and the beta-lactamase immobilized sponge is prepared. The beta-lactamase immobilized sponge (MS (at) beta-lactamase-CLEAs) is obtained, so that the antibiotics in the wastewater can be efficiently removed. By adopting the method disclosed by the invention, the stability and the catalytic activity of the beta-lactamase are enhanced, and the MS-coated beta-lactamase-CLEAs disclosed by the invention can be used for quickly and efficiently hydrolyzing beta-lactam antibiotics in wastewater and remarkably reducing the concentration of the beta-lactam antibiotics; compared with a traditional treatment method, the method has higher treatment efficiency and good reusability, a feasible solution is provided for treatment of wastewater polluted by antibiotics, and the method has wide application prospects.

The invention belongs to the field of functional fabrics, and particularly relates to a bio-based fabric with antibacterial and toughening functions and a preparation method thereof.The bio-based fabric comprises a fabric with bio-based fibers as a main body and a microcapsule antibacterial system fixed to the fabric; the microcapsule antibacterial system is composed of a core body and a wall material, and the core body of the microcapsule is composed of a bio-enzyme cross-linked enzyme aggregate and a stabilizer; the microcapsule wall material contains a component which forms a firm interface with the fiber; according to the invention, through a dual stabilization mechanism of CLEAs structure stabilization and microcapsule barrier protection on enzyme, the problems that free enzyme on the fabric is poor in stability and easy to fall off are solved, and the antibacterial activity of the enzyme is maintained for a long time. On the other hand, the strong binding force of the microcapsules and the fabric can improve the self strength of the fabric and improve the breaking strength and tearing strength of the bio-based fabric, meanwhile, the antibacterial property of the enzyme can effectively inhibit erosion and breeding of microorganisms to the bio-based fabric, and finally the effects of keeping clothes fresh for a long time and prolonging the service life of the fabric are achieved.

The application discloses a preparation method of a cross-linked enzyme aggregate biological catalytic membrane, and mainly comprises the following steps: taking a polyacrylonitrile ultrafiltration membrane as a base film, loading polydopamine modified UiO-66-NH2 nanoparticles to the surface of the polyacrylonitrile ultrafiltration membrane as an intermediate layer, the adsorption characteristics of the intermediate layer help to enrich micro-pollutants and promote enzyme catalysis, and loading cross-linked enzyme aggregates on the treated polyacrylonitrile ultrafiltration membrane by a pressure-assisted self-assembly technology, so as to obtain the cross-linked enzyme aggregate biological catalytic membrane. The preparation method is simple in operation and easy to implement. The cross-linked enzyme aggregate technology is used to combine separation, purification and immobilization together, one-step purification and immobilization of the enzyme are realized, and the immobilization amount is increased. The cross-linked enzyme aggregate biological catalytic membrane is used for removing micro-pollutants in water, separating dyes and salt ions in wastewater or removing methyl parathion in water, and the removal rate is more than 90%, so that the cross-linked enzyme aggregate biological catalytic membrane has good effects.

The application discloses a kind of preparation of carrier-free immobilized sucrose isomerase and its application in catalyzing sucrose to produce isomaltulose, and belongs to the field of bioengineering technology.The specific method of the application comprises the following steps: using polyethylene glycol as a precipitant, dextran aldehyde as a crosslinking agent, realizing the immobilization of sucrose isomerase by carrier-free immobilization-crosslinked enzyme aggregate method, adding bovine serum albumin as a stabilizer and amino donor in the preparation process, and then using the immobilized sucrose isomerase to convert sucrose raw materials to produce isomaltulose.The crosslinked sucrose isomerase aggregate prepared by the method has significantly improved temperature stability, pH tolerance and storage stability, and the enzyme activity recovery rate can be maintained at more than 90% after 10 times of continuous repeated use;after continuously preparing isomaltulose in batches, the substrate conversion rate of CLSIAs-BSA can be maintained at more than 85%.The application improves the efficiency of enzymatic preparation of isomaltulose, reduces production cost and separation and purification difficulty, is suitable for large-scale continuous production, and has great significance for improving the technical level of isomaltulose production in China.