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1 results about "Cross-linked enzyme aggregate" patented technology

In biochemistry, a cross-linked enzyme aggregate is an immobilized enzyme prepared via cross-linking of the physical enzyme aggregates with a difunctional cross-linker. They can be used as stereoselective industrial biocatalysts.

Preparation method and application of cross-linked enzyme aggregate biocatalytic membrane

ActiveCN117797667BCross-linked enzyme aggregateMethyl parathion
The application discloses a preparation method of a cross-linked enzyme aggregate biological catalytic membrane, and mainly comprises the following steps: taking a polyacrylonitrile ultrafiltration membrane as a base film, loading polydopamine modified UiO-66-NH2 nanoparticles to the surface of the polyacrylonitrile ultrafiltration membrane as an intermediate layer, the adsorption characteristics of the intermediate layer help to enrich micro-pollutants and promote enzyme catalysis, and loading cross-linked enzyme aggregates on the treated polyacrylonitrile ultrafiltration membrane by a pressure-assisted self-assembly technology, so as to obtain the cross-linked enzyme aggregate biological catalytic membrane. The preparation method is simple in operation and easy to implement. The cross-linked enzyme aggregate technology is used to combine separation, purification and immobilization together, one-step purification and immobilization of the enzyme are realized, and the immobilization amount is increased. The cross-linked enzyme aggregate biological catalytic membrane is used for removing micro-pollutants in water, separating dyes and salt ions in wastewater or removing methyl parathion in water, and the removal rate is more than 90%, so that the cross-linked enzyme aggregate biological catalytic membrane has good effects.
Owner:HEBEI UNIV OF TECH