The invention discloses a method for preparing soybean oil through enzymatic hydrolysis of an immobilized enzyme, and belongs to a plant oil extracting technology. The method comprises the following steps: (1) dissolving alkaline protease into a phosphate buffer solution, adding ethanol, precipitating and aggregating, adding glutaraldehyde, and crosslinking to obtain a crosslinked enzyme aggregate, namely immobilized alkaline protease; and (2) crushing soybeans, performing extrusion preprocessing to obtain an extruded material, crushing the extruded material, mixing the extruded material with water, regulating pH and temperature, adding the immobilized enzyme obtained in the step (1) for enzymatic hydrolysis, recycling the immobilized enzyme after the enzymatic hydrolysis, and centrifuging enzymatic hydrolysate to obtain the soybean oil. Processing equipment needed in the method is simple; the prepared immobilized enzyme is high in activity; the immobilizing time is short; a high-cost carrier is not required, so that the cost is greatly reduced; when the immobilized enzyme is applied to extraction of the soybean oil through the enzymatic hydrolysis, oil extracting rate is high; the immobilized enzyme can be recycled for reuse, so that resource waste is reduced; therefore, the method has very good economic benefit and development prospect.