The application discloses a preparation method of a cross-linked
enzyme aggregate biological
catalytic membrane, and mainly comprises the following steps: taking a
polyacrylonitrile ultrafiltration membrane as a base film, loading polydopamine modified UiO-66-NH2 nanoparticles to the surface of the
polyacrylonitrile ultrafiltration membrane as an intermediate layer, the adsorption characteristics of the intermediate layer help to enrich micro-pollutants and promote
enzyme catalysis, and loading cross-linked
enzyme aggregates on the treated
polyacrylonitrile ultrafiltration membrane by a pressure-assisted self-
assembly technology, so as to obtain the cross-linked enzyme aggregate biological
catalytic membrane. The preparation method is simple in operation and easy to implement. The cross-linked enzyme aggregate technology is used to combine separation, purification and immobilization together, one-step purification and immobilization of the enzyme are realized, and the immobilization amount is increased. The cross-linked enzyme aggregate biological
catalytic membrane is used for removing micro-pollutants in water, separating dyes and salt ions in
wastewater or removing
methyl parathion in water, and the removal rate is more than 90%, so that the cross-linked enzyme aggregate biological catalytic membrane has good effects.