Method for preparing soybean oil through enzymatic hydrolysis of immobilized enzyme

Abstract

The invention discloses a method for preparing soybean oil through enzymatic hydrolysis of an immobilized enzyme, and belongs to a plant oil extracting technology. The method comprises the following steps: (1) dissolving alkaline protease into a phosphate buffer solution, adding ethanol, precipitating and aggregating, adding glutaraldehyde, and crosslinking to obtain a crosslinked enzyme aggregate, namely immobilized alkaline protease; and (2) crushing soybeans, performing extrusion preprocessing to obtain an extruded material, crushing the extruded material, mixing the extruded material with water, regulating pH and temperature, adding the immobilized enzyme obtained in the step (1) for enzymatic hydrolysis, recycling the immobilized enzyme after the enzymatic hydrolysis, and centrifuging enzymatic hydrolysate to obtain the soybean oil. Processing equipment needed in the method is simple; the prepared immobilized enzyme is high in activity; the immobilizing time is short; a high-cost carrier is not required, so that the cost is greatly reduced; when the immobilized enzyme is applied to extraction of the soybean oil through the enzymatic hydrolysis, oil extracting rate is high; the immobilized enzyme can be recycled for reuse, so that resource waste is reduced; therefore, the method has very good economic benefit and development prospect.

Description

technical field [0001] The invention belongs to vegetable oil extraction technology, in particular to a method for preparing soybean oil by enzymatic hydrolysis of immobilized enzymes. Background technique [0002] In the process of extracting soybean oil by aqueous enzymatic method, the enzyme dissolves in the liquid phase and mixes with the material, and cannot be separated from the reaction system, which not only increases the cost, but also causes a waste of resources, and has a certain impact on product quality . Immobilized enzyme is a new technology developed in the 1960s. Generally, the enzyme is combined with an insoluble carrier, so that the enzyme is in a closed state in a certain space and can react continuously. After the reaction, the enzyme is passed through filtration or centrifugation, etc. The method is recycled and reused. [0003] However, the cost of reagents and carriers used for immobilization is high, the immobilization efficiency is low, and there ...

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Problems solved by technology

However, there is no report on the application of cross-linked enzyme aggregate technology-carrier-free immobilized enzyme technology to the extraction of soybean oil by aqueous enzymatic method. Low rate and other issues

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