The invention relates to a method for extracting maize germ oil by adopting an ultrasonic-assisted aqueous enzymatic method. The method for extracting the maize germ oil by adopting the ultrasonic-assisted aqueous enzymatic method is characterized by comprising the following steps: smashing maize germs taken as a raw material, sieving, then adding citric acid-sodium citrate buffer solution, and carrying out ultrasonic pretreatment; cooling, regulating pH value, then adding cellulose and alpha-amylase, stirring in constant-temperature water bath, and carrying out enzymatic hydrolysis; after the enzymatic hydrolysis is finished, deactivating enzymes, cooling to room temperature, centrifuging a sample, collecting supernate, demulsifying emulsion, centrifuging secondarily, then separating supernate, and mixing the supernates, so that the maize germ oil is obtained. The method for extracting the maize germ oil by adopting the ultrasonic-assisted aqueous enzymatic method has the advantages of mild conditions, high oil yield, no pollution, easy operation, safety, rapidness and applicability of large-scale production.