Method for synchronously extracting silybum marianum oil and hydrolyzed protein
A technology for hydrolyzing protein and extracting water, applied in the biological field, can solve the problems of protein variability, low oil yield, poor oil quality, etc., and achieve the effects of low extraction temperature, high oil extraction rate, and reduced production cost.
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Embodiment 1
[0026] Select qualified milk thistle seeds, use mechanical crushing, and the crushed material is passed through a 60-mesh sieve; the crushed milk thistle powder and water are mixed evenly with a mass ratio of 1:3, and the mixture of papain and amylase is added. Compound enzyme, the mass ratio of compound enzyme to milk thistle powder is 0.005:1; the mass ratio of papain to amylase is 1:1, under the conditions of pH 6.5 and temperature 35°C, stirring and enzymolysis for 1 hour, enzyme The solution mixture was inactivated at a temperature of 95 °C for 10 min;
[0027] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle...
Embodiment 2
[0029] Select milk thistle seeds with qualified quality, and adopt mechanical crushing, and the crushed material is passed through a 60-mesh sieve. Mix the pulverized milk thistle powder and water evenly at a mass ratio of 1:5, add a compound enzyme of papain and amylase, the mass ratio of the compound enzyme to milk thistle powder is 0.02:1; papain and starch The mass ratio of the enzyme is 1:1. Under the conditions of pH 6.8 and temperature 45°C, stir the enzymolysis for 2 hours, and heat the enzymolysis mixture at 95°C for 10 minutes;
[0030] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle oil, and then combi...
Embodiment 3
[0032] Select milk thistle seeds with qualified quality, and adopt mechanical crushing, and the crushed material is passed through a 60-mesh sieve. Mix the pulverized milk thistle powder and water evenly at a mass ratio of 1:7, add a compound enzyme of papain and amylase, the mass ratio of the compound enzyme to milk thistle powder is 0.035:1, papain and starch The mass ratio of the enzyme is 1:1. Under the conditions of pH 7.2 and temperature 55°C, stir the enzymolysis for 3 hours, and heat the enzymolysis mixture at 95°C for 10 minutes;
[0033] The enzymolysis mixture was centrifuged at 4000 r / min for 15 min. After centrifugation, the first layer of clear oil, the second layer of emulsion, the third layer of protein hydrolyzate and the fourth layer of residue were obtained, and the clear oil was obtained by absorbing layer by layer. , emulsion and protein hydrolyzate. The emulsion was added petroleum ether at a volume ratio of 1:3 to extract milk thistle oil, and then comb...
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