82 results about "Campesterol" patented technology

The invention discloses a method for measuring sterol in tobaccos. The method comprises the following steps of: (1) deploying sterol standard templates containing internal standard with different concentrations, carrying out GC-MS (Gas Chromatography-Mass Spectrometer) analysis after derivatization, and plotting by utilizing the ratio of the sterol to internal standard peak area and the ratio of the sterol to internal standard concentration to obtain a standard curve; (2) carrying out acid hydrolysis, saponification and direct ultrasonic extraction on samples to be measured, and taking 1 muL for carrying out GC-MS analysis after derivatization; and (3) reading the ratio of the sterol to the internal standard concentration from the standard curve by utilizing the ratio of the sterol to theinternal standard peak area, and then calculating the amount of the sterol. The invention can be used for analyzing cholesterol, ergosterol, campesterol, stigmasterol and beta-sitosterol with a plurality of forms in tobacco leaves at the same time, has the advantages of accurate measurement and simple and convenient operation, and can be widely applied to tobacco industry.

The present invention provides an isolation process of the pure sterols from the SODD by simple acid catalyzed esterification and the separation of the reagents by distillation and water washing followed by the crystallization of phytosterols and purification. Soybean oil deodorizer distillate (SODD) and distillate from other vegetable oil refining contain free sterols, in addition to steryl esters, tocopherols squalene and unknown compounds. The process is also applicable to other vegetable oil distillate containing more than 1% phytosterols in the free form. Phytosterols thus obtained is of high purity (95-99%) and high yield (80-90%) and contains β-sitosterol, Stigmasterol and campesterol.

The invention discloses a method for simultaneously determining stigmasterol, campesterol, beta-sitosterol and squalene in vegetable oil. The method comprises the following steps of: 1) saponifying the vegetable oil, and extracting an unsaponifiable matter in a saponification solution by using an organic solvent; and 2) dissolving the unsaponifiable matter in the organic solvent, and determining by using high-performance liquid chromatography, wherein the high-performance liquid chromatography has the chromatographic conditions that a chromatographic column is a C18 column, a mobile phase is a solvent of which the volume ratio of acetonitrile to water is (90:10) to (100:0), an ultraviolet detection wavelength is 205 to 210 nm, a sample size is 10 to 20 mu L, flow speed is 1.0 to 1.5 mL/min and column temperature is 25 to 30 DEG C. By adoption of the method, effective separation of the campesterol, the stigmasterol, the beta-sitosterol and the squalene in the vegetable oil can be simultaneously realized, consumed time for separation is about 40 min, and components in a chromatogram are eluted in a sequence of the campesterol, the stigmasterol, the beta-sitosterol and the squalene.

The invention discloses a method for extracting phytosterol from waste residues generated in biodiesel production, comprising the following steps of: converting phytosterol in a combined state into free phytosterol through deep saponification; extracting the phytosterol through an organic solvent to prepare mother liquor of crude sterine; recovering the solvent to obtain a crude sterine product, wherein the extraction rate of the crude sterine can reach 88.6 percent; recrystallizing the crude sterine product through absolute ethyl alcohol to obtain high-purity phytosterol, wherein the purity of the obtained phytosterol can reach 91.3 percent, and the prepared phytosterol contains campesterol, stigmasterol and sitosterol. The invention has simple extraction and purification method, changes the produced waste residues of biodiesel into valuables and has a good industrial application prospect.

Disclosed are: a fat-reduced soymilk having a reduced fat content, which is produced by separating a fat from a fat-containing soybean efficiently without relying on an organic solvent; and a novel fat-rich soybean material. Specifically disclosed are: a fat-reduced soybean protein material characterized by containing a protein and a carbohydrate at a total content of 80 wt % or more in terms of dried form content, containing a fat (as an extract from a chloroform/methanol mixed solvent) at a content of less than 10 wt % relative to the content of the protein, and containing campesterol and stigmasterol (as plant-derived sterols) at a total content of 200 mg or more relative to 100 g of a fat; and a soybean emulsion composition characterized by containing a protein at a content of 25 wt % or more in terms of dried form content, containing a fat at a content of 100 wt % or more in terms of dried form content relative to the content of the protein in terms of dried form content, and having an LCI value of 60% or more.

The present invention addresses the problem of providing a soybean-derived raw material-containing food or beverage that improves problems with flavor and physical properties, such as the grassy smell caused by soybean raw materials, and markedly improves product quality, in a soybean-derived raw material-containing food or beverage using a conventional soybean raw material such soymilk or tofu. Provided are a milk-substitute composition, an egg yolk-substitute composition, and a composition for improving renal function, etc., characterized by including a reduced-fat soybean protein material having a total protein and carbohydrate content relative to dry material of at least 80 wt %, a fat content (as a chloroform/methanol mixed solvent extract) relative to the protein content of less than 10 wt %, and a total campesterol and stigmasterol (as plant-derived sterols) content of at least 200 mg relative to 100 g of fat. Also provided are a variety of soybean-derived raw material-containing food and beverages using these compositions.

The invention relates to the technical field of genetic engineering and particularly discloses a recombinant yarrowia lipolytica bacterial strain as well as a construction method and an application thereof. A genome of the recombinant yarrowia lipolytica bacterial strain comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-3. The construction method comprises the following steps of constructing a key gene dhcr7 in a biosynthesis way of campesterol expressed by a single-gene expression cassette through an OE-PCR (overlap extension-polymerase chain reaction) method; cutting down the single-gene expression cassette through restriction enzyme, transferring the single-gene expression cassette into yeast at one time to perform homologous recombination among fragments and integration of a special erg5 lotus of the genome, screening a completely-assembled converter through an auxotrophic culture medium and genome PCR to obtain a novel recombinant yarrowia lipolytica bacterial strain. The novel recombinant yarrowia lipolytica bacterial strain can be applied to biosynthesis of campesterol, and a relatively high yield can be kept.

The invention discloses a method for separating and testing campesterol, stigmasterol and beta-sitosterol by using a high performance liquid chromatography method. The method comprises the following steps: preparing a standard solution, namely preparing a mixed standard standby solution with campesterol, stigmasterol and beta-sitosterol from solar solvents, treating the mixed standard standby solution, and further respectively preparing mixed standard standby solutions with campesterol, stigmasterol and beta-sitosterol from solar solvents. By adopting the method, three sterol monomers are subjected to base line separation, the retention time is greatly shortened, an optimal spectrum condition of 1.0mL/minute is finally confirmed, the detection wavelength is 208nm, the column temperature is35 DEG C, the method is relatively high in both precision and sample feeding recycling rate, and the detection requirement that contents of three sterol monomers are accurately and quantitatively detected is met.

The invention provides a food additive for cleaning and keeping fresh, which comprises the following ingredients in parts by weight: 0.2-18% of ethyl maltol, 3-12% of eucalyptus essential oil, 20-48% of Sapinberry fruit peel extract, sodium bicarbonate 1-6%, 0.01-10% of pothos radix extract, 3-5% of campesterol, 1-4% of edible salt, 5-15 parts of sodium glycinate, 4-8 parts of glutamic acid, 4-8 parts of aloe extract, 4-16 parts of wheat starch, the rest is water, when the food additive used for cleaning and fresh-keeping fruits and vegetables of the present invention cleans and keeps fresh fruits and vegetables, it can remove the insufficiency of the original chemical cleaning agent cleaning, and can effectively avoid food pollution. Its composition is food grade, High safety factor.

The invention relates to recombinant yarrowia lipolytica T30pED with high yield of campesterol as well as a construction method and application of the recombinant yarrowia lipolytica T30pED. The strong promoter TEF30 provided by the invention can be used for constructing a recombinant strain of the yarrowia lipolytica, and verification shows that the highest yield of the campesterol in shake flask fermentation reaches 664.1 mg/L (33.21 mg/g DCW) and the highest yield of the campesterol in a 5L fermentation tank reaches 1305.2 mg/L (68.73 mg/g DCW), which are obviously higher than the yield of a wild type promoter strain, so that the strong promoter has outstanding industrial application significance.

The invention relates to a tobacco 7-dehydrocholesterol reductase gene and application thereof. The nucleotide sequence of the 7-dehydrocholesterol reductase gene is as shown in SEQ ID NO. 1. Based on the important influence of sterol on the safety of tobacco leaves, the tobacco sterol metabolism regulation gene is deeply studied, so that a new tobacco variety is constructed by utilizing genetic engineering, and a good application foundation is laid for improving the tobacco variety. According to the application, preliminary research on specific tobacco sterol 7-dehydrocholesterol reductase shows that the specific tobacco sterol 7-dehydrocholesterol reductase is highly related to the sterol content in tobacco leaves, when the gene is silenced in Bensi tobacco, the stigmasterol content and the campesterol content in the tobacco leaves are reduced, and the total sterol content is reduced by 24.8%. Based on the characteristic, a certain foundation and reference can be provided for tobacco quality regulation and control and new tobacco variety cultivation.

The invention discloses a lubricating agent for a textile machine and a preparation method of the lubricating agent. The lubricating agent for the textile machine is prepared from components in parts by weight as follows: 50-60 parts of lubricating oil, 4-6 parts of chlorinated paraffin, 3-6 parts of talc powder, 2-3 parts of 2-ethyl hexylphosphic mono(2-ethylhexyl) ester, 2-3 parts of benzoyl peroxide butyl acetate, 1-2 parts of magnesium zinc ferrite, 1-2 parts of campesterol, 0.5-1 part of hydroxymethyl acrylamide, 0.5-0.6 parts of dimethylol urea, 0.2-0.4 parts of 4-hydroxyl-3-methoxybenzoic acid and 0.2-0.4 parts of trimethylamine acetate. The invention further provides the preparation method of the lubricating agent for the textile machine.

The invention relates to a method of preparing high-purity monomer cycloartenyl ferulate, 24-methylene cycloartenyl ferulate, campesterol ferulate and sitosterol ferulate from a rice bran extract through separation by counter-current chromatography. The four active substances such as high-purity cycloartenyl ferulate are prepared from the rice bran extract through separation by the counter-currentchromatography; a solvent systems comprises three ingredients: petroleum ether or normal paraffin, dichloromethane and acetonitrile or fatty alcohol at a volume ratio of 10:(0-3):(7-12); the method is applicable to preparation of monomer cycloartenyl ferulate, 24-methylene cycloartenyl ferulate, campesterol ferulate and sitosterol ferulate by using various models of counter-current chromatographs; many crude products or synthetic mixtures can be introduced directly; the purity of separated cycloartenyl ferulate and sitosterol ferulate reaches above 95%; and the purity of 24-methylene cycloartenyl ferulate and campesterol ferulate reaches above 90%.

A solubilizing agent of the formula €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ X-OOC-(CH 2 ) n -COO-Y wherein X is a member selected from campesterol, sitosterol, ergosterol and stigmasterol; Y is a member selected from polyalcohol, polyether, polyanion, polycation, polyphosphoric acid, polyamine, polysaccharide, polyhydroxy compound and polylysine, and derivatives thereof; and n is an integer selected from 0 to 18. The solubilizing agent may be included in a water-soluble composition comprising a bioactive lipophilic compound and the solubilizing agent.

The invention discloses a pollution-free plant pesticide, comprising following materials in part by weight: 3-6 parts of campesterol, 5.5-7.5 parts of cinnamon oil, 2.8-4.5 parts of fatty alcohol-polyoxyethylene ether, 5-8 parts of xanthate gum, 4.5-5.6 parts of sodium acrylate, 1.8-4.5 parts of aluminum magnesium silicate and 13.5-15 parts of water. The pesticide has the advantages that the toxicity is less, residues are not basically generated, no harmlessness is caused to people, livestock and plants, no pesticide resistance is caused to pests, and the like, has contact killing and stomach toxicity functions to various pests, does not need adsorption and fumigation action, is low in cost, simple in process, free from pollution in he preparation process, easy in material collection and beneficial to utilizing tobacco waste in a cigarette factory, and lightens environment pollution; and the extracted raw material residues can be as fertilizer or can be developed to feed.

The invention provides a method for preparing five ferulate single components by taking soapstock containing oryzanol as a raw material. The method comprises the following steps: 1) carrying out alkali-alcohol hot dissolution and filtering while the soapstock is hot; 2) carrying out acid precipitation and filtration; 3) degreasing and decoloring by using a lipophilic organic solvent; and (4) preparing thin-layer chromatography for separation; wherein the condition is that the content of the oryzanol in a precipitate crude product obtained after decoloring in the step (3) is 70-80wt%. The method is characterized in that soapstock with the oryzanol content of 10-20% is used as a raw material, a product with the oryzanol content of 70-80 wt% is obtained through a specific pretreatment purifying step, and then a monomer substance is separated and prepared through thin-layer chromatography; the purity of the five prepared monomers, namely 24-methylene cycloartanyl ferulate, cycloartenyl ferulate, cycloartanyl ferulate, campesterol ferulate and beta-sitosterol ferulate is greater than 90%; and the ferulate single components can be used as a reference substance for content determination,and can be used for external standard method content calculation and qualitative peak position calibration.

The invention belongs to the field of food inspection and detection, and discloses a camellia oleosa seed oil adulteration detection method based on oleic acid/behenic acid and beta-amyrin/campesterolratios. Camellia oleosa seed oil and different vegetable oils are saponified, saponified substances are subjected to fatty acid content detection, non-saponified substances are subjected to triterpenoid compound detection, and through two ratios of oleic acid/behenic acid to beta-amyrin/campesterol, the content of fatty acid in the saponified substances is detected. Adulteration qualitative identification analysis can be rapidly carried out on rice oil, sunflower seed oil, corn oil, rapeseed oil, soybean oil, peanut oil and novel high-oleic-acid vegetable oil (high-oleic-acid peanut oil, high-oleic-acid sunflower seed oil and high-oleic-acid rapeseed oil) which are common in the market at present. According to the method, the adulteration in the pure tea oil can be judged through comprehensive judgment of two indexes, namely the ratio of methyl cis-oleate to methyl behenate is smaller than 1200 or the ratio of beta-amyrin to campesterol is smaller than 40, the adulteration characteristic of the pure tea seed oil can be visually presented, and the adulteration of the pure tea seed oil can be qualitatively identified.

The invention discloses saccharomyces cerevisiae engineering bacteria for producing campesterol and a construction method, and belongs to the field of genetic engineering and bioengineering. An expression cassette element of 7-dehydrocholesterol reductase is introduced into a yeast cell body, and is integrated into a yeast genome by utilizing a CRISPR/Cas9 gene operating system, C-22 sterol desaturase is knocked out, competition of an ergosterol branch path is relieved, and yeast synthesis from glucose to campesterol is realized. The method is simple in process, green and environment-friendly, and can be used for producing campesterol through fermentation. The yield of campesterol prepared by adopting the saccharomyces cerevisiae engineering strain BY4742-delta erg5-TEF1p-dhcr7-ADH1t provided BY the invention can reach 253.35mg/L in a shake flask stage, and the horizontal yield of a fermentation tank can reach 916.88mg/L, which is increased by 2.6 times compared with the yield of campesterol in the shake flask stage of the strain.