27 results about "Campesterol" patented technology

The invention provides a culture method for improving immunity of fishes. The culture method comprises the following steps: S1, selection of culture points; S2, specification and layout of net cages; S3, selection and throwing of fish fries; according to the fry disinfection method disclosed by the invention, the fry are soaked and disinfected by using a compound disinfectant prepared from povidone iodine, polysorbate, citric acid and vitamin C in a specific proportion, so that pathogenic microorganisms on the surfaces and gill parts of fish bodies are effectively killed. According to the breeding management, the immunity of the fish body is comprehensively enhanced by feeding the fish with an immune conditioner composed of campesterol palmitate, immunomodulatory peptide, beta-glucan and astaxanthin in a specific proportion. According to the bottom mud management, the content of harmful substances is reduced by putting a bottom mud modifier composed of sodium molybdate, nitrifying bacteria, photosynthetic bacteria, tetrandrine and sodium alginate in a specific proportion, growth of pathogenic bacteria is inhibited, nutrient substance circulation is promoted, the water quality environment is optimized, and healthy growth of fry is promoted.

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The invention relates to corn with the efficacy of preventing or improving osteoporosis symptoms. The mass ratio of campesterol to stigmasterol to beta-sitosterol in the corn is 1: (0.5-5): (1-5). The planted corn can significantly improve osteoporosis, and has the advantages of high yield of planted corn and corn straw, good taste, convenience in use, high bioavailability, safety, effectiveness, high comprehensive utilization rate of resources, greenness, environmental protection and the like.

The invention discloses a method for increasing the yield of pregnenolone in heterologous synthesis of yarrowia lipolytica. According to the method, a recombinant yarrowia lipolytica strain is constructed, and the construction method is selected from one of the following three methods: on the basis of the recombinant yarrowia lipolytica strain SyBEY12060077, CYP11A1 and an ER positioning sequence KDEL are fused; the CYP11A1 is integrated, and meanwhile, the additional Adx gene expression is increased; the CYP11A1 is integrated, and the CYB5 gene is introduced at the same time. According to the newly constructed strain, acquisition of precursor campesterol and electron transfer of P450 enzyme are improved, so that the overall steroid flux is improved, the yield of heterologous production of pregnenolone is improved, and a research thought is provided for subsequent optimization of metabolic regulation and improvement of synthesis of recombinant yarrowia lipolytica steroids.

The application provides a camellia oil adulteration identification method and system, which comprises the following steps: obtaining the content of oleic acid, beta-amyrin and campesterol in the camellia oil to be identified; determining whether the content of oleic acid is greater than a first threshold value; if yes, determining that the camellia oil to be identified is a mixture of one or more of camellia oil, high-oleic peanut oil, high-oleic sunflower oil and high-oleic rapeseed oil; if no, determining that the camellia oil to be identified is a mixture of one or more of peanut oil and soybean oil; then determining whether the content of beta-amyrin causes the displacement amount of the clustering result of the corresponding camellia oil to be less than a second threshold value; if yes, determining that the camellia oil to be identified is not adulterated camellia oil; if no, determining that the camellia oil to be identified is adulterated oil; then determining whether the content of campesterol causes the displacement amount of the clustering result of the corresponding high-oleic rapeseed oil to be less than a third threshold value, and determining whether the camellia oil to be identified is camellia oil adulterated with high-oleic rapeseed oil.

The application relates to the field of cosmetics, and relates to an anti-aging cosmetic, grape pomace oil and a preparation method thereof. The anti-aging cosmetic comprises grape pomace oil; characteristic components of the grape pomace oil include oleanolic acid, alpha-guaiacwoodene, beta-tocotrienol, beta-sitosterol, campesterol, soybean sapogenol E, ganoderic acid F, ganoderol I and D-erythro-dihydrosphingosine; the total phenol content of the grape pomace oil is greater than or equal to 1 mg / g, the total sterol content of the grape pomace oil is greater than or equal to 26 mg / g, and the total flavonoid content of the grape pomace oil is greater than or equal to 1 mg / g; the grape pomace oil is extracted from grape pomace; the grape pomace is mainly composed of grape skin, grape stem and grape seed. The compounds have excellent antioxidant properties and can improve the anti-aging effect of the anti-aging cosmetic. The compounds are helpful for protecting the skin from oxidative damage and play a positive role in cosmetics. The grape pomace oil maximally retains active components of all parts of the grape pomace.

The invention discloses a protein composition and application thereof in production of steroids. The protein combination is composed of a DWF protein, a CYP protein and an HSD protein, the DWF protein is an MtDWF5 protein or an StDWF5 protein, the CYP protein is an MtCYP108 protein, an MtCYP150 protein, a CaCYP108-1 protein, a CaCYP108-2 protein, a CaCYP150 protein or a DpCYP108 protein, and the HSD protein is an MtHSD6 protein, a synMtHSD5 protein, a CaHSD5 protein, a PsHSD5 protein or an SlHSD5 protein. Experiments prove that the protein combination can be used for producing at least one of campesterol, cholesterol, pregnenolone and progesterone by taking 7-dehydrosterol as a substrate. The method has important application value.

The invention discloses a saccharomyces cerevisiae strain with high yield of campesterol as well as a construction method and application of the saccharomyces cerevisiae strain. One of the key rate-limiting enzymes, which are heterologously synthesized in the yeast, of the campesterol ((24R)-Ergosta-5-en-3beta-ol) is from a plant C-24 (28) reductase DWF1 mutant (Ar207). In view of the characteristics that the catalytic activity of the enzyme is relatively low and substrates are dispersedly distributed in different compartments of cells, on the basis of the original recombinant saccharomyces cerevisiae for producing campesterol, a rate-limiting enzyme gene copy positioned to a specific subcellular organelle is additionally added, so that the campesterol can be produced. The expression quantity is effectively increased; meanwhile, the contact between the enzyme and a substrate in a corresponding chamber is enhanced; and the yield of campesterol is increased. By utilizing the strategy, Ar207 is positioned to recombinant bacteria of peroxisome and lipid droplets, and the yield of campesterol reaches 3.00 g / l and 3.77 g / l respectively during fermentation in a 5L tank.

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The application discloses a plant source campesterol Yarrowia lipolytica production strain and a construction method. 12 The application is constructed as follows: (1) taking Yarrowia lipolytica strain AHH 7 as a chassis, introducing Δ 24 \-sterol-C5(6)-desaturase gene 28 _DWF7, 7-dehydrocholesterol reductase gene At _DWF5 and Δ At \-sterol reductase gene Ar _DWF1 from Arabidopsis thaliana, and obtaining a recombinant strain Yl001; (2) recovering a URA3 nutritional marker in the strain Yl001, integrating the genes ARE1 and the URA3 nutritional marker into an ERG4 site of the Yl001, and knocking out the gene ERG4, to obtain a plant source campesterol Yarrowia lipolytica production strain Yl006; the yield of the plant source campesterol of the strain Yl006 is increased by 21.83%, reaching 343.34 mg / L.

The invention relates to corn capable of bidirectionally regulating anxiety and depression symptoms. The mass ratio of campesterol to stigmasterol to beta-sitosterol in the corn is 1: (0.5-5): (1-5). The planted corn can bidirectionally regulate anxiety and depression symptoms, and has the advantages of high yield of planted corn and corn straw, good taste, convenience in use, high bioavailability, safety, effectiveness, high comprehensive utilization rate of resources, environmental friendliness and the like.

The invention belongs to the quality standard and detection technology field of health food, and is specifically related to a detection method for measuring the total amount of multiple free sterols and sterol esters in a kind of plant sterol ester. The present invention utilizes C18 solid phase extraction cartridge purification technology, separates and removes other components, enriches target component, utilizes reversed-phase liquid chromatography, first quantitatively detects the free sterol content originally present in four kinds of raw materials of stigmasterol, campesterol, β-sitosterol, and rapeseed sterol; Then weigh the sample and carry out saponification to convert sterol ester into sterol, with methanol-water-phosphoric acid mixed solvent as mobile phase A, methanol-isopropanol mixed solvent as mobile phase B, methanol as mobile phase C, gradient elution is carried out through reversed-phase C18 analytical column, the total amount of sterols in the determination sample (including the free sterols originally present and the sterols obtained by saponification and hydrolysis) is determined, so as to convert the content of plant sterol ester in raw material.

The present invention relates to the technical field of detection methods, discloses a method for distinguishing true and false, adulterated camellia oil, comprising the following steps: adopting gas chromatography, taking campesterol and butyryl cetol as characteristic indicators for analysis, true camellia oil sample can not carry out qualitative and quantitative analysis on campesterol, can carry out qualitative and quantitative analysis on butyryl cetol, content is 1100~2000 mg / kg, thereby distinguishing true and false, adulterated camellia oil; wherein, butyryl cetol is analyzed by gas chromatography external standard method, and carrier gas is nitrogen; split ratio: 20‑40:1; column temperature adopts programmed temperature rise: 180‑190°C is maintained for 1‑5min, then warmed to 260‑300°C at 10‑15°C / min, maintained for 20‑40min. The present invention can quickly distinguish true and false, adulterated camellia oil by selecting campesterol and butyryl cetol as characteristic indicators, and the method is simple, the detection limit is low, and the accuracy rate is high.

The invention relates to the technical field of genetic engineering, in particular to a recombinant bacterium for increasing the yield of (22R, 23R)-22, 23-dihydroxy-7-dehydrocampesterol and application of the recombinant bacterium. The recombinant bacteria disclosed by the invention are saccharomyces cerevisiae YQC512 expressing truncated cytochrome P450 reductase, cytochrome P450 enzyme from flax and cytochrome P450 enzyme from peanut. Compared with the existing engineering bacteria, the engineering bacteria provided by the invention have the advantage that the synthesis capability of the (22R, 23R)-22, 23-dihydroxy-7-dehydrocampesterol is improved. Furthermore, an expression cassette for expressing GAL4 by using a sucrose inducible promoter PSUC2 is integrated into a saccharomyces cerevisiae YQC512 genome, so that the engineering bacteria can be strongly expressed under the culture condition that sucrose is used as a unique carbon source, and the safety risk caused by using ethanol as a carbon source in the existing fermentation production process is reduced.

A method for feeding invertebrates or aquaculture organisms, comprising # providing a pollen substitute composition comprising a nutritionally effective amount of isofucosterol, fucosterol or a mixture thereof; and # administering the pollen substitute composition to invertebrates or aquaculture organisms; wherein the pollen substitute composition comprises a nutritionally effective amount at least one further sterol, preferably at least two further sterols selected from the group consisting of cholesterol, 24-Methylene-cholesterol, campesterol, stigmasterol and beta-sitosterol or a physiologically available conjugate of any of these sterols.

The present invention relates to the field of genetic engineering technology, and in particular to a recombinant bacterium for increasing the production of (22R, 23R)-22,23-dihydroxy-7-dehydrorapesterol and its application. The recombinant bacterium of the present invention is a saccharomyces cerevisiae YQC512 expressing a truncated cytochrome P450 reductase, a cytochrome P450 enzyme derived from sesame, and a cytochrome P450 enzyme derived from peanut. The engineered bacterium provided by the present invention has improved the ability to synthesize (22R, 23R)-22,23-dihydroxy-7-dehydrorapesterol compared to existing engineered bacteria. Furthermore, the present invention converts the sucrose-inducible promoter P SUC2 The expression cassette for GAL4 was integrated into the genome of Saccharomyces cerevisiae YQC512, enabling the engineered bacteria to express strongly under culture conditions with sucrose as the sole carbon source, reducing the safety risks of using ethanol as a carbon source in existing fermentation production processes.

The invention relates to a method for replacing cholesterol in Eriocheir sinensis feed with modified phytosterol. According to the method, 0.045%-0.064% by mass of microcapsule-coated modified phytosterol is added into the eriocheir sinensis compound feed to replace part or all of cholesterol. The main components of the modified phytosterol comprise beta-sitosterol, stigmasterol, campesterol and campesterol, and the modified phytosterol has high water solubility and bioavailability. The feed is used for feeding the eriocheir sinensis in a growing period, a fattening period or an early breeding period after being extruded and granulated. Application results show that the method can effectively improve the gonad index, the estradiol content and the reproductive performance of the eriocheir sinensis and optimize the feed cost structure. The method is easy and convenient to operate, raw materials are easy to obtain, and the method is suitable for the fields of cholesterol replacement and functional feed development in aquaculture.

The present disclosure relates to lipid emulsions for parenteral administration, comprising 10 to 30% of a lipid phase comprising soybean oil, medium-chain triglycerides (MCT), olive oil and fish oil and less than 25 mg, preferably less than 20 mg, campesterol, less than 30 mg, preferably less than 25 mg, stigmasterol and less than 120 mg, preferably less than 100 mg, betasitosterol per 100 g of the lipid phase. The present disclosure also relates to the lipid emulsions according to the present disclosure for providing parenteral nutrition, in particular to neonates, as well as to methods for manufacturing the lipid emulsions according to the present disclosure.

The invention provides a camellia oil adulteration identification method and system. The method comprises the following steps: acquiring the oleic acid content, beta-amyrin content and campesterol content of to-be-identified camellia oil; judging whether the oleic acid content is greater than a first threshold; if yes, determining that the to-be-identified camellia oil is one or a mixture of more of camellia oil, high-oleic-acid peanut oil, high-oleic-acid sunflower seed oil and high-oleic-acid rapeseed oil; if not, determining one or a mixture of more of peanut oil and soybean oil; then judging that the content of beta-amyrin enables the displacement of the clustering result of the corresponding camellia oil to be smaller than a second threshold value; if yes, determining that the to-be-identified camellia oil is undoped camellia oil; if not, determining that the to-be-identified camellia oil is doped oil, judging whether the displacement amount of displacement of the clustering result of the corresponding high-oleic-acid rapeseed oil caused by the campesterol content is smaller than a third threshold value or not, and determining whether the to-be-identified camellia oil is the camellia oil doped with the high-oleic-acid rapeseed oil or not.

The present application belongs to the technical field of feed additive, and particularly relates to a compound fatty acid preparation for promoting chicken growth, and a preparation method and application thereof. The compound fatty acid preparation for promoting chicken growth is mainly composed of the following raw materials in parts by weight: 3-6 parts of stigmasterol, 2-4 parts of campesterol, 10-20 parts of linoleic acid, 12-24 parts of tributyrin, 1-2 parts of emulsifier, 3-5 parts of soybean phospholipid, 3-5 parts of protective agent, and 30-80 parts of water. The combination of stigmasterol and campesterol can significantly improve the targeted release efficiency of linoleic acid and tributyrin. The released butyric acid can activate the mitochondrial beta-oxidation pathway of intestinal epithelial cells, significantly improve the efficiency of tricarboxylic acid cycle, and enhance the intestinal barrier function. The phytosterol can also competitively inhibit the NPC1L1 transporter, reduce the intestinal cholesterol absorption, direct the energy to the growth demand, thereby improving the daily weight gain of broilers and reducing the feed-meat ratio.

The invention provides application of a feed additive in improving growth performance, glycolipid metabolism capability, oxidation resistance and / or iridovirus resistance of mandarin fish. The feed additive comprises 63-67 parts of beta-sitosterol, 23-27 parts of campesterol and 8-12 parts of stigmasterol. When the feed additive is applied to mandarin fish compound feed, the mandarin fish growth speed and the feed utilization efficiency can be increased, the mandarin fish glycolipid metabolism capability is enhanced, the mandarin fish oxidation resistance and the iridovirus challenge survival rate are improved, and the sustainable development of the mandarin fish culture industry is promoted.

The invention relates to corn capable of improving the sex hormone level. The mass ratio of campesterol to stigmasterol to beta-sitosterol in the corn is 1: (0.5-5): (1-5). The corn can significantly improve the sex hormone level, thicken the vaginal mucosa, significantly reduce the pathological score and effectively relieve the climacteric symptoms of patients, and has the advantages of high yield of planted corn and corn straw, good taste, convenience in use, high bioavailability, safety, effectiveness, high comprehensive utilization rate of resources, environmental protection and the like.

The present invention relates to genetically-modified oleaginous yeasts for producing non-native sterols at commercially useful levels, especially for example in providing sterols individually or as a mixture in an artificial dietary composition for honeybees or other insects or animals. For this purpose, an oleaginous yeast, e.g. Yarrowia lipolytica, may be employed wherein the yeast has reduced production of ergosterol compared with a wild-type oleaginous yeast or is incapable of producing ergosterol and is provided with a sterol surrogate to aid growth. From such yeast, however, other yeast may be engineered which retain useful sterol production without need for a sterol surrogate, e.g. production of sterol mixtures in which 24-methylenecholesterol or campesterol is the dominant sterol.

Provided are methods or processes for producing ferulic acid from a plant material, for example, a rice bran or its derivatives. Provided are methods comprising an ion swapping and solvent extraction process followed by a chromatographic separation operations that are coupled into a process which functions to recover a fraction rich in gamma-oryzanol, thus enabling the subsequent production of a high purity ferulic acid. Provided are methods comprising an ion swapping and solvent extraction process followed by a process which functions to recover a fraction rich in gamma-oryzanol, or a mixture of ferulic acid esters of phytosterols and triterpenoids, optionally comprising cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and / or campesteryl ferulate, to enable the production of a high purity ferulic acid. Provided are methods comprising a saponification and solvent extraction process followed by recovering a fraction rich in gamma-oryzanol to enable the production of a high purity ferulic acid.