Method for simultaneously determining phytosterol and squalene in vegetable oil

Abstract

The invention discloses a method for simultaneously determining stigmasterol, campesterol, beta-sitosterol and squalene in vegetable oil. The method comprises the following steps of: 1) saponifying the vegetable oil, and extracting an unsaponifiable matter in a saponification solution by using an organic solvent; and 2) dissolving the unsaponifiable matter in the organic solvent, and determining by using high-performance liquid chromatography, wherein the high-performance liquid chromatography has the chromatographic conditions that a chromatographic column is a C18 column, a mobile phase is a solvent of which the volume ratio of acetonitrile to water is (90:10) to (100:0), an ultraviolet detection wavelength is 205 to 210 nm, a sample size is 10 to 20 mu L, flow speed is 1.0 to 1.5 mL / min and column temperature is 25 to 30 DEG C. By adoption of the method, effective separation of the campesterol, the stigmasterol, the beta-sitosterol and the squalene in the vegetable oil can be simultaneously realized, consumed time for separation is about 40 min, and components in a chromatogram are eluted in a sequence of the campesterol, the stigmasterol, the beta-sitosterol and the squalene.

Description

technical field

[0001] The invention relates to a method for simultaneously determining phytosterol and squalene in vegetable oil. Background technique

[0002] Phytosterol is a natural active substance that widely exists in plants. There are many kinds of natural phytosterols, mainly including β-sitosterol, stigmasterol, and campesterol. Phytosterols are widely found in various vegetable oils, nuts and plant seeds, and also in other plant foods such as vegetables and fruits. The content of sterols in different plant species is different, such as wheat germ oil, corn germ oil, rice bran oil, The content in flower oil is relatively high, and in the daily intake, phytosterols from cereals, vegetables, and fruits can account for more than 20% of the total intake. The human body cannot synthesize phytosterols freely and can only be ingested from food. Phytosterols have high safety and can be used as new food additives. The chemical structure of phytosterol is similar to that ...

Images