Method for measuring sterol in tobaccos

A method for measuring sterols, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cholesterol cannot be measured at the same time, and achieve the effect of convenient quantitative determination, simple operation and accurate test
CN101963604AInactive Publication Date: 2011-02-02CHINA TOBACCO SICHUAN IND CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA TOBACCO SICHUAN IND CO LTD
Publication Date
2011-02-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for measuring sterol in tobaccos. The method comprises the following steps of: (1) deploying sterol standard templates containing internal standard with different concentrations, carrying out GC-MS (Gas Chromatography-Mass Spectrometer) analysis after derivatization, and plotting by utilizing the ratio of the sterol to internal standard peak area and the ratio of the sterol to internal standard concentration to obtain a standard curve; (2) carrying out acid hydrolysis, saponification and direct ultrasonic extraction on samples to be measured, and taking 1 muL for carrying out GC-MS analysis after derivatization; and (3) reading the ratio of the sterol to the internal standard concentration from the standard curve by utilizing the ratio of the sterol to theinternal standard peak area, and then calculating the amount of the sterol. The invention can be used for analyzing cholesterol, ergosterol, campesterol, stigmasterol and beta-sitosterol with a plurality of forms in tobacco leaves at the same time, has the advantages of accurate measurement and simple and convenient operation, and can be widely applied to tobacco industry.
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Description

technical field

[0001] The present invention relates to the determination method of sterol, especially the determination method of sterol in tobacco. Background technique

[0002] Sterols are a class of important compounds in tobacco. They are considered to be the precursors of condensed aromatic hydrocarbons in cigarette smoke. They have a great impact on the quality and safety of tobacco. A comprehensive understanding of the content and form of sterols in tobacco can reduce the harm of cigarettes. Focus reduction has certain guiding significance. Sterols account for about 0.1-0.3% of the weight of tobacco, mainly including campesterol, stigmasterol, cholesterol and β-sitosterol, and moldy tobacco leaves also contain ergosterol. The first four sterols mainly exist in the forms of esters, glycosides, acyl sugar compounds and free forms. The currently reported quantitative analysis method for the simultaneous determination of free and bound sterols in tobacco is mainly gas ...

Claims

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