41 results about "Ergosterol" patented technology

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The invention relates to a polydextrose-containing powder electuary and a preparation method thereof, and belongs to the technical field of functional foods. The formula of the instant granules comprises 30 to 50 parts of polydextrose powder, 5 to 15 parts of camellia flower extract, 10 to 30 parts of corn starch, 0.1 to 1 part of ergosterol, 1 to 5 parts of konjac glucomannan and 10 to 20 parts of deionized water. The camellia extract is prepared through a specific process, and the specific process comprises the steps of drying, grinding, ethanol ultrasonic extraction, microwave extraction, freeze drying and the like, so that the extract with high purity and fine granularity is obtained. The preparation method comprises the following steps: uniformly mixing the raw materials, adding the deionized water and the camellia extract, stirring, homogenizing, pre-freezing, freeze-drying and the like. Homogenization treatment and pre-freezing treatment parameters are optimized to ensure the uniformity and stability of the product. The finally obtained powder electuary is uniform in particle size, has good solubility and taste, and is suitable for being used as a nutritional supplement or functional food.

The invention discloses a cynanchum bungei fermented and purified C21 steroidal glycoside for resisting hepatic fibrosis. Through cell and animal experiments, it is found that the C21 steroidal glycoside of the radix cynanchi bungei fermented and purified product can inhibit proliferation of precursor collagenous cells; the hardness value of the liver of a model mouse and the content of hydroxyproline in the liver tissue can be remarkably reduced, key anti-hepatic fibrosis related index data are superior to that of ergosaponin commonly used at present, and the application prospect of the bunge auriculate root fermentation purified product C21 steroidal glycoside in preparation of anti-hepatic fibrosis drugs or fibrosis treatment drugs is wide.

The invention relates to the technical field of household appliances, and provides refrigeration equipment. According to the embodiment of the invention, the refrigeration equipment comprises an equipment main body, a door body and a nutrient increasing bin. The door body can cover the equipment main body; the nutrient increasing bin is arranged on the door body or the equipment main body, a nutrient increasing space is formed in the nutrient increasing bin, the nutrient increasing bin is provided with an ultraviolet lamp, the nutrient increasing space is used for containing target food materials containing ergosterol, and the ultraviolet lamp is suitable for converting the ergosterol of the target food materials into vitamin D. According to the refrigeration equipment disclosed by the invention, the nutrition increasing bin is arranged, so that ergosterol contained in a target food material placed in the nutrition increasing space can be converted into vitamin D required by people through the ultraviolet lamp, and a user can obtain the target food material with high vitamin D content more conveniently; the target food materials can be used as food materials for cooking after being subjected to nutrient enrichment of the nutrient enrichment bin, so that the refrigeration equipment is convenient to operate, and an effective storage function can be provided.

The invention discloses marine red yeast for efficiently co-producing astaxanthin, carotenoid and ergosterol as well as a co-production process and application of the marine red yeast. According to the invention, a brand new marine rhodotorula mucilaginosa HY-25 is screened and identified, a culture process of the marine rhodotorula mucilaginosa HY-25 is provided, and a process for co-producing astaxanthin, carotenoid and ergosterol through fermentation of the strain is provided. By adopting the fermentation process disclosed by the invention, the amounts of the carotenoid, the astaxanthin and the ergosterol which are co-produced respectively reach 25.45 mg / L, 1.32 mg / L and 55.43 mg / L. Meanwhile, xylose and glucose in the bamboo hydrolysate can be utilized by the strain, the utilization rate of the glucose reaches 90% or above, and the utilization rate of the xylose reaches 30% or above; the strain can adapt to different salt environments, so that the strain can be fermented in sugar-containing wastewater liquid, and high-added-value products are produced by utilizing effective components in the wastewater.

The invention discloses a method for preparing agaricus bisporus powder rich in vitamins, which comprises the following steps: step 1, selecting fresh agaricus bisporus which is free from mechanical injury and plant diseases and insect pests and has complete sporocarp, cleaning, and soaking in a color protection solution for color protection; 2, slicing the agaricus bisporus subjected to color protection treatment, and freeze-drying the sliced agaricus bisporus to obtain a dry agaricus bisporus product; and step 3, irradiating the agaricus bisporus dry product under the UV-B wave band with the wavelength of 280-320 nm, so that ergosterol in the agaricus bisporus dry product is converted into vitamins, and the agaricus bisporus powder rich in the vitamins is obtained. According to the invention, color-protecting fresh-keeping pretreatment is combined with a freeze-drying technology, so that nutritional active ingredients and flavor substances of agaricus bisporus can be reserved to the maximum extent, and the content of vitamin D2 is remarkably increased; compared with UVA and UVC, UVB can significantly improve the content of vitamin D2, the whole process is completed by single UV radiation, the preparation process does not need a UV light source switching step, and the energy consumption is low.

The present invention provides a method for preparing a Cordyceps militaris composite walnut protein health conditioning product. Fresh Cordyceps militaris is used as the raw material, which is treated with an electron beam, pulped, and then a polysaccharide monooxygenase is added to the product. The product is freeze-dried to obtain modified Cordyceps militaris powder, which is then fused with walnut protein using a stage-controlled technique. As a nutritionally functional ingredient, the modified Cordyceps militaris powder overcomes the defects of walnut milk, which are prone to stratification and quality deterioration. The use of fresh Cordyceps militaris increases the nutritional value of the product, improving its content of dietary fiber, Cordyceps polysaccharides, cordycepin, and ergosterol. The active ingredients, particularly ergosterol, are effectively maintained during digestion, thereby improving absorption and bioavailability. The preparation technology and product not only help control blood sugar but also neutralize gastric acid and improve indigestion.

The present disclosure provides a method for preparing vitamin D2 using a microchannel reactor, including: (a) dissolving ergosterol in a first solvent to form a first reaction solution; (b) introducing the first reaction solution into the microchannel reactor, and the first reaction solution is irradiated with light source A and light source B in sequence as the first reaction solution passes through a first photoreaction zone and a second photoreaction zone; (c) collecting and concentrating the first reaction solution irradiated by the light source B; (d) optionally, mixing the first reaction solution of step (c) with a second solvent to form a second reaction solution; (e) heating the first reaction solution of step (c) or the second reaction solution of step (d) to obtain the vitamin D2.

The invention discloses an oligonucleotide aptamer M8 for high-specificity recognition of ergosterol and verification of the oligonucleotide aptamer M8, and belongs to the field of molecular biomedicine. The nucleotide sequence of the oligonucleotide aptamer M8 is as shown in SEQ ID NO. 1. The method comprises the following steps: screening random nucleotides by using an ergosterol solution, continuously replacing a reverse screening target, finally screening to obtain a plurality of oligonucleotide aptamer sequences, sequencing, carrying out sequence alignment, constructing a family tree according to homology, determining four oligonucleotide aptamer sequences, and carrying out specificity and affinity verification. The result shows that the oligonucleotide aptamer M58 has strong affinity and high specificity to the ergosterol, and the affinity and the specificity of the oligonucleotide aptamer M58 to the ergosterol are verified by the oligonucleotide aptamer M8 obtained by truncation. The invention can be used for early diagnosis of ergosterol fungal infection.

A Ganoderma lucidum triterpene composition and use thereof in the preparation of a drug and health-care food for clearing senescent cells and resisting tumors. The Ganoderma lucidum triterpene composition comprises ≥30% total triterpene, ≥1.8% ganodermanontriol, ≥0.1% ganoderic acid X, ≥0.7% ganodermanondiol, ≥0.7% ganoderol B, ≥2.5% ganoderol A, ≥0.2% ganoderal A, and ≥0% ergosterol.

The invention discloses a method for converting ergosterol into vitamin D2 by using DES and a method for enriching vitamins. The method for converting the ergosterol into the vitamin D2 comprises the following steps: obtaining an ergosterol solution by taking a deep eutectic solvent as a solvent, and then placing the ergosterol solution under UVB ultraviolet rays to carry out light conversion, so that the ergosterol is converted into the vitamin D2. The method can improve the conversion rate and stability of ergosterol. The method for enriching vitamins comprises the following steps: (1) obtaining mushroom powder; (2) carrying out ultrasonic-assisted extraction on the mushroom powder by using a natural hydrophobic deep-eutectic solvent, and after the extraction is finished, centrifuging and taking supernate, so as to obtain an ergosterol extracting solution; and (3) placing the ergosterol extracting solution under UVB ultraviolet rays to carry out light conversion so as to obtain a solution rich in vitamin D2. According to the method, the extraction efficiency of ergosterol and the conversion rate and stability of vitamins are remarkably improved.

The invention relates to the technical field of biology, in particular to construction and application of a recombinant strain for synthesizing veratrazine and steroid precursors of the veratrazine, and the steroid precursors comprise squalene, ergosterol, lanosterol, 22R-hydroxy cholesterol, 22R, 26-dihydroxy cholesterol and 22-hydroxy-26-amino-cholesterol. The recombinant strain provided by the invention can efficiently express steroid precursors, for example, the content of lanosterol reaches 172mg / L and is increased by about 170 times compared with that of an original strain, the yield of 22R-hydroxycholesterol reaches 3482.83 mu g / L, the yield of 22R, 26-dihydroxycholesterol reaches 1003.05 mu g / L, veratrazine can be efficiently synthesized, and the yield of veratrazine reaches 265.38 mu g / L.

The invention provides a volvariella volvacea mutant strain VLH1-18 and an application of the volvariella volvacea mutant strain VLH1-18. The bacterial strain is preserved in Guangdong Microbial Culture Collection Center on July 31, 2025, the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 66785, and the bacterial strain is classified and named as Volvariella volvacea. The strain can realize artificial cultivation, and the formed sporocarp has excellent shape and excellent postharvest storage stability. Mycelium shows obvious stress resistance characteristics, the tolerance to high temperature and low temperature is obviously enhanced, and the resistance to infectious microbe infection is obviously improved. Compared with the original strain, the contents of polysaccharide, flavonoid, ergosterol and vitamin C in the volvariella volvacea mutant strain are respectively increased by 62.29%, 106.78%, 147.21% and 29.41%, the nutritional quality is obviously improved, and the mutant strain has important value in variety improvement and marketization application of edible mushrooms. The invention further provides an artificial cultivation method of the volvariella volvacea mutant strain. The artificial cultivation method comprises the steps of mother strain manufacturing, production strain manufacturing, sowing and fruiting management.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

Provided is a fungus synthesizing 24-epi-ergosterol, a construction method therefor, and use thereof. A sterol Δ24(28) reductase DWF1 gene or a mutant gene thereof is recombinantly expressed in a fungal body producing 24(28)-dehydroergosterol fungal body producing ergosterol, and the strain synthesizing 24-epi-ergosterol is constructed. By means of directed evolution of DWF1, DWF1 has an increased ability to reduce a unpreferable substrate 24(28)-dehydroergosterol to form 24-epi-ergosterol in the fungus, and in combination with high-density fermentation, the yield of 24-epi-ergosterol is improved; by means of the use of a synthetic biology strategy that enhances esterification and hydrolysis to enhance sterol homeostasis, the metabolic flux towards 24-epi-ergosterol in the fungus is increased; the promoters of DWF1 mutant and ACC1 genes are optimized, and ERG5 is overexpressed, so that the proportion of 24-epi-ergosterol in sterols is increased, and in combination with the high-density fermentation, the yield of 24-epi-ergosterol is significantly improved.

The invention discloses a cultivation material for increasing the vitamin content of straw mushrooms and application of the cultivation material in straw mushroom cultivation. The cultivation material is formed by mixing and fermenting the following components in percentage by weight: 82-82.8% of waste cotton, 8% of bran, 4% of corn flour, 2% of light calcium carbonate, 3% of lime and 0.2-1% of ergosterol. According to the cultivation method, the content of vitamin A, vitamin D2 and vitamin D3 in straw mushroom sporocarps is remarkably increased, the yield is guaranteed, meanwhile, the nutritional value of straw mushrooms is increased, and the cultivation method is suitable for large-scale industrial production.

The invention discloses a method for preparing a laying hen feed additive by fermenting tea-seed oil deodorized distillate, which is characterized in that the tea-seed oil deodorized distillate is used as a raw material, and efficient utilization of all components of resources is achieved by coupling yeast-paecilomyces cicadae two-step sequential fermentation and endogenous mevalonic acid (MVA) pathway cascade regulation and control technology. The final product is rich in ergosterol, vitamin E and probiotics, can improve the laying rate of laying hens and enhance the eggshell strength after being applied to laying hen breeding, realizes zero wastewater discharge in the process, and achieves the dual goals of resource utilization of grease byproducts and accurate matching of laying hen nutrition.

The invention relates to the field of synthesis of organic fluorescent dyes, and discloses a conjugate of indocyanine green and cholesterol as well as synthesis and application of the conjugate. The conjugate is formed by connecting an indocyanine green derivative and a cholesterol derivative through an amido bond; the indocyanine green derivative is ICG-R-COOH; iCG is indocyanine green, R is-(CH2) nH. [I-], n is an integer of 1-6, or R is-(CH2) mSO3-, and m is 3 or 4; and the cholesterol derivative is cholesterole-NH2, beta-sitosterole-NH2, stigmasterole-NH2 or ergosterole-NH2. The invention also discloses a preparation method of the cholesterol derivative. The lipidosome constructed by the conjugate is high in stability, good in dispersity and strong in NIR-II fluorescence, the blood vessel and tumor imaging contrast ratio and definition under a 1300 nm near-infrared imaging window are superior to those of lipidosome constructed by commercial ICG, more excellent diagnosis and treatment performance is shown, and wide application prospects are achieved.

The invention provides a method for detecting the content of ergosterol in lentinus edodes based on near infrared spectroscopy and application. And rapidly detecting the content of the ergosterol in the shiitake mushrooms by adopting a near infrared spectrum technology, and inputting spectral data of a sample to be detected into the optimal model to obtain a predicted value of the ergosterol, so that the prediction of the content of the ergosterol in the shiitake mushrooms is realized. According to the method, the SG smoothing algorithm and the normalization algorithm are combined to preprocess the spectrum, noise generated when the sensor obtains the spectrum data can be effectively removed, and baseline and scattering correction is carried out; the variable combined population analysis-genetic algorithm is used for carrying out characteristic wavelength extraction on an original spectrum, so that required important variables can be effectively extracted, redundant information is efficiently removed, and the operation rate of the model is improved; the content of ergosterol in shiitake mushrooms is predicted by adopting the least square support vector machine model based on the crown porcupine optimization algorithm, and the model is high in adaptive capacity and high in prediction accuracy. And a new technical approach is provided for rapidly detecting the content of ergosterol.

The invention discloses a Cordyceps sinensis powder strain capable of producing beneficial components and application thereof, and belongs to the technical field of microorganisms, the Cordyceps sinensis powder strain Gsxcf1 is preserved in China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.41894, the preservation date is April 21, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Cordyceps farinosa strain Gsxcf1 provided by the invention is separated from wild cordyceps sinensis, after liquid fermentation or solid fermentation, the content of active ingredients such as polysaccharide, ergosterol and cordycepin is high, and the Cordyceps farinosa strain Gsxcf1 has great potential to be applied to development of cordyceps sinensis sporocarp or mycelium related food.

The present application belongs to the technical field of edible and medicinal mushroom detection, and particularly relates to a characteristic spectrum construction method of deer mushroom extract and a method for determining the content of a marker effective component. The method is detected by high performance liquid chromatography, and the characteristic spectrum and the content of the effective component ergothioneine can be determined and analyzed by one chromatographic condition. The method comprises the following steps: taking formic acid acetonitrile solution as mobile phase A, taking formic acid solution as mobile phase B, performing gradient elution through a specific elution program, obtaining 9 characteristic peaks of ergosterol and ergothioneine, and quantitatively detecting the effective component ergothioneine by an external standard method. The method is simple in operation, short in detection time, and has good specificity, repeatability, precision and stability, can fully reflect the integrity and characteristics of deer mushroom and its extract, is suitable for the characterization and quality evaluation of the whole components of deer mushroom, and provides technical support and scientific basis for the full development and utilization of deer mushroom resources.

The invention provides ganoderma lucidum spore oil as well as a preparation method, a detection method and application thereof, and the ganoderma lucidum spore oil is prepared by adopting a three-phase separation and extraction method: suspending ganoderma lucidum spore powder by 10mL of 50% ammonium sulfate, adding a tert-butyl alcohol solution according to a material-liquid ratio of 1: 15, uniformly mixing, adjusting the pH value of the suspension to 5, performing ultrasonic-assisted extraction for 30 minutes, extracting for 2.5 hours in an oscillating water bath kettle at 50 DEG C, centrifuging for 15 minutes by 10000 g, and performing vacuum filtration to obtain the ganoderma lucidum spore oil. Dividing into three layers, taking the upper layer, carrying out rotary evaporation to remove tert-butyl alcohol, blowing with nitrogen until no alcohol smell exists, and weighing to obtain the ganoderma lucidum spore oil extraction rate; according to the detection method, the compounds in the ganoderma lucidum spore oil can be accurately and quantitatively detected, the ganoderma lucidum spore oil rich in triterpenic acid, fatty acid and ergosterol can be extracted through the three-phase separation and extraction method, and the ganoderma lucidum spore oil has a remarkable hepatocyte injury resisting effect.

The present invention discloses an antimicrobial peptide with anti-Candida albicans activity and its application, belonging to the field of biomedicine technology. The amino acid sequence of the antimicrobial peptide is NH2-GFKKLKLFKLKPF-COOH. The minimum inhibitory concentration of the antimicrobial peptide is 4.0 μM; the antimicrobial peptide is prepared into nanovesicles with egg yolk lecithin, cholesterol, and DSPE-MPEG2000, and its minimum inhibitory concentration is 2.0 μM. The heptamer oligomerization structure formed by the phenylalanine (Phe)-rich region in the antimicrobial peptide of the present invention can, on the one hand, specifically target ergosterol in the fungal cell membrane and significantly enhance the anti-Candida albicans activity; on the other hand, it can destroy the rigid conformation of the α-helix by inserting proline (Pro) residues, increase the molecular flexibility to penetrate the thick cell wall of the fungus, thereby enhancing its core mechanism of action against Candida albicans, and can also effectively treat fungal vaginitis without developing drug resistance.

This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.

The present invention relates to the field of pharmaceutical chemistry, and specifically to a method for preparing cholesterol, a derivative thereof, and an analog thereof. The derivative of cholesterol comprises, but is not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25-hydroxy-7-dehydrocholesterol, and ergosterol. In the present invention, a compound represented by formula I can be prepared by means of a microbial transformation using phytosterols as a raw material, followed by preparing cholesterol, the derivative thereof, and the analog thereof.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses bacillus paralicheniformis 2-12, a biocontrol inoculant and application of the biocontrol inoculant. The bacillus parlicheniformis 2-12 is preserved in the China Center for Type Culture Collection on December 19, 2023, the preservation number of the bacillus parlicheniformis 2-12 is CCTCC NO: M 20232598, and the bacillus parlicheniformis 2-12 is classified and named as bacillus parlicheniformis 2-12. The bacillus paralicheniformis 2-12 provided by the invention can generate antibacterial volatile organic compounds, plays a bacteriostatic role by destroying the form of pathogenic bacteria hyphae and reducing the content of ergosterol, can effectively replace chemical pesticides, and is used for biological prevention and control of plant root rot.

The invention provides mitochondria-phospholipid gel as well as a preparation method and application thereof, and belongs to the technical field of low-temperature medicine. The mitochondria-phospholipid gel is prepared from the following raw materials: mitochondria, high-molecular sodium hyaluronate, trehalose, egg yolk lecithin, ergosterol and deionized water. The phospholipid gel prepared from high-molecular sodium hyaluronate, trehalose, egg yolk lecithin and ergosterol has a protective effect on mitochondria and can maintain the activity and morphological structure of the mitochondria, so that when the mitochondria-phospholipid gel acts on damaged nerves, nerve regeneration and repair can be promoted.

The invention belongs to the technical field of detection of edible and medicinal fungi, and particularly relates to a characteristic spectrum construction method of a pilose antler mushroom extract and a method for determining the content of symbolic effective components. According to the method, high performance liquid chromatography is adopted for detection, and determination and analysis of the specific chromatogram and the content of the effective component ergothioneine can be achieved through one chromatographic condition. Comprising the steps that a formic acid acetonitrile solution serves as a mobile phase A, a formic acid solution serves as a mobile phase B, gradient elution is conducted through a specific elution procedure, nine characteristic peaks such as ergosterol and ergothioneine are obtained, the effective component ergothioneine is quantitatively detected through an external standard method, and the method is easy to operate and short in detection time and has good specificity, repeatability, precision and stability; the method can fully reflect the integrity and characteristics of the pilose antler mushroom and the extract thereof, is suitable for integral component characterization and quality evaluation of the pilose antler mushroom, and provides technical support and scientific basis for full development and utilization of pilose antler mushroom resources.

The invention discloses an antibacterial peptide with anti-Candida albicans activity and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the antibacterial peptide is NH2-GFKKLKLFKLKPF-COOH, and the amino acid sequence of the antibacterial peptide is shown in the description. The minimum inhibitory concentration of the antibacterial peptide is 4.0 mu M; the antibacterial peptide, egg yolk lecithin, cholesterol and DSPE-MPEG2000 are prepared into nano-vesicles, and the minimum inhibitory concentration of the nano-vesicles is 2.0 [mu] M. According to the antibacterial peptide disclosed by the invention, a heptamer oligomerization structure is formed in a phenylalanine (Phe) enrichment region, so that on one hand, ergosterol of a fungal cell membrane can be specifically targeted, and the anti-candida albicans activity is remarkably improved; on the other hand, the rigid conformation of alpha-helix can be destroyed by inserting proline (Pro) residues, and the molecular flexibility is increased to penetrate through thick cell walls of fungi, so that the anti-candida albicans core action mechanism is enhanced, fungal vaginitis can be effectively treated, and drug resistance cannot be generated.