244 results about "Ergosterol" patented technology

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention discloses a method for measuring sterol in tobaccos. The method comprises the following steps of: (1) deploying sterol standard templates containing internal standard with different concentrations, carrying out GC-MS (Gas Chromatography-Mass Spectrometer) analysis after derivatization, and plotting by utilizing the ratio of the sterol to internal standard peak area and the ratio of the sterol to internal standard concentration to obtain a standard curve; (2) carrying out acid hydrolysis, saponification and direct ultrasonic extraction on samples to be measured, and taking 1 muL for carrying out GC-MS analysis after derivatization; and (3) reading the ratio of the sterol to the internal standard concentration from the standard curve by utilizing the ratio of the sterol to theinternal standard peak area, and then calculating the amount of the sterol. The invention can be used for analyzing cholesterol, ergosterol, campesterol, stigmasterol and beta-sitosterol with a plurality of forms in tobacco leaves at the same time, has the advantages of accurate measurement and simple and convenient operation, and can be widely applied to tobacco industry.

Significant concentrations of naphthalene were detected in carton nests of Formosan subterranean termites, Coptotermes formosanus Shiraki, collected from Florida, Hawaii, and Louisiana. This is the first report of naphthalene being associated with termites or any other insects. Naphthalene and other compounds associated with termite carton nests may be used to increase termite bait acceptance. New attractant molecules include 2-phenoxyethanol. New feeding stimulants include ergosterol. A list of volatile compounds associated with termite nests is presented, compounds that may be used to detect termite nests.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention discloses a method of yeast fermentation coproduction ergot sterol and glutathion, utilizing conspecific yeast bacterial fermentation to produce simultaneous two kinds of metabolite-ergot sterol and glutathion, the utilization bacterial of fermentation is Saccharomyces cerevisiae, candida utilis, candida tropicalis or engineering bacterial reconstructed by mutagenesis and gene engineering, the utilization yeast culture medium contain the carbon source such as glucose, cereal mash, sucrose molasses and so on, the nitrogen source such as maize milk, yeast powder, protease leather, carbonyldiamide, ammonia and so on, inorganic salt and the metallic ion that the yeast need. The invention utilizes fermentation coproduction ergot sterol and glutathion, simultaneously, accomplish high-density culture of the yeast cell, and make the biomass of yeast expression coproduction come up to the standard of the high level in individual fermentation ergot sterol and glutathion, full utilization culture medium substrate, increasing availability ratio of raw material, decreasing the cost of manufacture.

The present invention relates to the field of pharmaceutical chemistry, and in particular to methods of preparing cholesterol,and derivatives and analogs thereof. The cholesterol derivatives include, but not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25- hydroxy7dehydrocholesterol and ergosterol. In the invention, phytosterol can be used as a raw material to prepare the compound shown in the formula I through microbial conversion, and then cholesterol and the derivatives and analogues thereof are prepared.

A novel use is disclosed of liposomes that contain phytosterol (e.g., β-sitosterol, campesterol, stigmasterol, brassicasterol, ergosterol, and ergostadienol, and so forth). It has been newly discovered that liposomes that contain phytosterol have a cancer metastasis inhibiting activity. Cancer metastasis is inhibited by the oral administration of liposomes that contain phytosterol and these liposomes are therefore useful for the prevention and treatment of cancer.

The invention relates to an anti-virus feed additive for reducing cholesterol, a production process thereof and application, which belong to the technical field of biological products. The additive comprises the following effective components in percentage by weight: 90 to 95 percent of unsaturated fatty acid, 1 to 5 percent of glucosamine, 1.5 to 5 percent of organic acid, 1.5 to 5 percent of ergosterol and 1 to 6 percent of beta glucanase. The process flow comprises the following steps: (1) performing sterilization by fermentation equipment and sterile air filtration equipment; (2) cultivating bacterial strains; (3) fermenting; (4) extracting; and (5) spray-drying. The additive is added into feed of table poultries, laying hens, pigs, meat quails, egg quails, meat pigeons and laying pigeons in a small amount, can reduce meat and egg cholesterol of livestock and poultry effectively, reasonably and safely, has the anti-virus function, is not added with any Chinese or western medicinalpreparations and chemical compositions in the production process, and is a pure microbial fermentation additive.

The invention discloses a novel synthesis method for the raw material medicine of nicergoline (shown in formula I). The novel synthesis method disclosed by the invention comprises the following steps of: reacting ergosterol with 5-bromonicotinate derivatives to generate carboxylic ester; then performing a photocatalytic reaction with methanol in an acidic condition; and then performing a methylation reaction with a methylation reagent to generate nicergoline. With the adoption of the novel synthesis method disclosed by the invention, the cumbersome operation of the methods disclosed by the existing patents is avoided, the used solvent is low in toxicity, and the yield is high. The novel synthesis method is beneficial to industrialized popularization and application.

The invention discloses a preparation method of a dydrogesterone intermediate. The preparation method is characterized by comprising the following steps: dissolving 5,7-diene steroid compound in an organic solvent to obtain a solution as a raw material; and carrying out a photocatalytic reaction and separating to obtain the dydrogesterone intermediate, wherein the 5,7-diene steroid compound is 7-dehydropregnenolone acetate, pregna-5,7-diene-3,20-diketodivinyl ketal, 7-dehydropregnenolone, ergosterol or pregna-5,7-diene-3,20-diketo-3-vinyl ketal, and a lamp used in the photocatalytic reaction comprises an LED ultraviolet lamp with the wavelength range of 295-335 nm. The preparation method of the dydrogesterone intermediate is high in yield, low in cost, safer in preparation and friendlier to environment.

The invention discloses a production method of ergosterol. The production method includes the following steps: adding an extracting solvent to a dried Monascus purpureus-fermented sample, extracting by an extraction process to obtain crude extract, eluting the crude extract by column chromatography to obtain primary purified eluent, subjecting the primary purified elution to silica gel thin-layer chromatography, combining eluents with same silica gel thin-layer chromatography results and performing column chromatography again to obtain secondary purified eluent, removing the solvent from the secondary purified eluent, and recrystallizing through petroleum ether to obtain ergosterol. The preparation of ergosterol from Monascus purpureus as material is provided for the first time, crude extract of the sample is extracted first, and an optimal column chromatography eluent is screened then according to distribution conditions and dissolutions in different solvents, so that ergosterol is isolated and purified effectively. The production method of the ergosterol is simple, easy to implement and low in time consumption, the material is easy to obtain and low in cost, and batch production of ergosterol is achieved.

The invention belongs to the field of pharmaceutical intermediate and particularly discloses a method for extracting ergosterol from a starch yeast. The method comprises the following steps: (1) adding a solvent and a saponifying agent in the starch yeast containing the ergosterol to obtain to-be-filtered liquid I; (2) cooling and filtering the to-be-filtered liquid I; (3) adding an extracting agent in filter residues and stirring to obtain to-be-filtered liquid II; (4) cooling and filtering the to-be-filtered liquid II; (5) distilling a filtrate to obtain a crystal substance; (6) collecting the crystal substance, adding an extracting agent to re-dissolve, adding a coloring agent and stirring to obtain to-be-filtered liquid III; (7) filtering the hot to-be-filtered liquid III, collecting afiltrate and slowly cooling to precipitate out an ergosterol crude product; (8) adding a re-crystallization solvent in the collected ergosterol crude product, heating, then slowly reducing the temperature and collecting a crystal substance to obtain an ergosterol product. The method disclosed by the invention is short in short process; less in resource waste, resource-saving; high in product purity, less in harmful impurity and capable of environmental protection.

The invention discloses a preparation method of ginkgo leaf lipoid components having antibacterial activities. Ginkgo leaf which is a raw material is extracted with a non-polar solvent, and undergoes molecular distillation separation, column chromatography, re-crystallization and purification to obtain eight compounds (sequentially comprising isophytol, nerolidol, linalool, beta-sitosterol acetate, beta-sitosterol, stigmasterol, ergosterol and daucosterol), polypentenol, ginkgo leaf lipoid total unsaponifiable matters, ginkgo leaf lipoid crystals, a ginkgo leaf lipoid light fraction and a ginkgo leaf lipoid heavy friction. The eight compounds, polypentenol, the ginkgo leaf lipoid total unsaponifiable matters, the ginkgo leaf lipoid crystals, the ginkgo leaf lipoid light fraction and the ginkgo leaf lipoid heavy friction have inhibition effects on salmonellas, Staphylococcus aureus and Aspergillus niger.

The present invention relates to a fermentation method, particularly to a method for produce monascus by using distillers' grain fermentation. The method specifically comprises the following steps: (1) preparing a distillers' grain saccharification liquid matrix: crushing dried distillers' grains to achieve 40-80 meshes, adding water to the crushed distillers' grains according to a weight ratio of the distillers' grains to the water of 1:10-20, uniformly mixing, heating to a temperature of 85-95 DEG C, adding alpha-amylase to carry out liquefaction for 20-30 min, cooling to a temperature of 55-65 DEG C, adding glucoamylase, and carrying out thermal insulation saccharification for 4-6 h to obtain a distillers' grain saccharification liquid; (2) preparing a solid state fermentation culture medium: adopting the distillers' grain saccharification liquid obtained from the step (1) to prepare a solid state fermentation culture medium, wherein the addition amount of the distillers' grain saccharification liquid is 15-32% of the total weight of the solid state fermentation culture medium; and (3) carrying out fermentation culture on monascus sp. The monascus prepared by using the method contains a plurality of physiological activity substances, further contains monascus pigment, ergosterol, gamma-aminobutyric acid and unsaturated fatty acids, and has a good health care effect, wherein amino acid content achieves 20%, and MonacolinK content can achieve 4-30 mg/g.

The invention discloses a saccharomyces ergosterol high-producing strain, and a breeding method and application for the same. The saccharomyces ergosterol high-producing strain is named as saccharomyces cerevisiae scut-B6305 and is preserved in the China Center for Type Culture Collection in Wuhan, China, the preservation date is April 10, 2012, and the preservation number is CCTCC (China Center for Type Culture Collection) NO:M2012105. A saccharomyces lysate producing strain is continuously subjected to stress oriented domestication by dimethyl sulfoxide for a long time so as to breed the saccharomyces ergosterol high-producing strain. The content of ergosterol in unit cell of the saccharomyces ergosterol high-producing strain reaches 5.63mg/g and is increased by 30.32% as compared with the original strain, the production capacity of unit volume of fermentation liquor of the saccharomyces ergosterol high-producing strain is 90.8mg/100mL and is 1.91 times of that of unit volume of fermentation liquor of the original strain, and the saccharomyces ergosterol high-producing strain can be used for obtaining the ergosterol during production.

The invention discloses a preparation method of an RGD peptide and penetrating peptide R8 co-modified ergosterol and cis-platinum active drug-loading liposome. The method comprises the main steps of ergosterol liposome preparation, ammonium chloride gradient formation, drug loading and co-modification. The method is simple in technology and easy to carry out, and the encapsulation rate of a drug is high; ergosterol is adopted to partially replace cis-platinum to exert efficacy, an ergosterol and cis-platinum active drug-loading liposome is obtained, the effect of resisting a lung cancer is guaranteed, the toxic and side effects of the drug are significantly reduced, and damage to human bodies is small; meanwhile, the RGD peptide and the penetrating peptide R8 serve as target heads, the targeting property is good, and the drug exertion effect is good.

The invention discloses application of ergosterol and ergosterol liposome prepared from the ergosterol, and particularly relates to the application of the ergosterol on treating lung cancer, and the ergosterol liposome for treating the lung cancer. In the ergosterol liposome, the drug loading capacity of the ergosterol is 5-20 wt%; the liposome consists of lecithin and cholesterol, wherein the mole ratio of the lecithin to the cholesterol is (1:1)-(7:1). The ergosterol liposome disclosed by the invention has remarkable lung cancer resistant effects, low cytotoxicity, small damage to human bodies, and target ability.

The invention relates to a method for photochemically synthesizing vitamin D2 and D3 in a tubular reactor. The method is characterized in that the vitamin D2 and D3 are synthesized by ultraviolet light of different bands, the residence time of reaction liquid fed at different times in the tubular reactor is controlled to be the same by not dissolving with reaction liquid, not participating in reaction liquid separation and carrying ergosterol and 7-dehydrocholesterol reaction liquid and enhancing internal disturbance of the reaction liquid; the method has the advantages of continuous, stable and high efficiency of solar energy utilization, the operation is simple, and the process is controllable; the method has great prospect in the industrial production of the photochemically synthesizedvitamin D2 and D3.

The invention provides a truffle hydrophilic solvent extractive and a drug, food and the health food thereof containing the truffle hydrophilic solvent extractive. The preparation method of the truffle hydrophilic solvent extractive includes the step that a hydrophilic solvent is used for extracting truffle, and the truffle hydrophilic solvent extractive includes ergosterol and the fat type and has an obvious ultraviolet absorption at 195 to 220nm wavelength position. The invention also provides the application in preparing the drug for curing prostate disease, male sexual disorder and low immunizing power of a human body or the food or the health food for improving the associative function thereof by taking the extractive of the truffle in a hydrophilic solvent as the active ingredient.

The invention relates to a method for photochemosynthesis of vitamin D2, which comprises dissolving lysergol into a mixed system of nonpolar-polar solvent at the presence of nitrogen protection, charging anti-oxidant, controlling temperature of the photochemical reaction solution at 23-30 deg C, guaranteeing high concentration with mixed solvent, controlling conversion rate of the lysergol below 35%, using non-reacted lysergol as the raw material for next photochemical reaction. The process can substantially increase the concentration of the reactants.

The invention relates to novel polyenes having formula (I), wherein: R1 represents alkyl C₁-C₃; and R2 represents a functional group selected from CH₃— or CONH₂— (methyl- or primary amide-). The aforementioned polyenes have a biocide action on organisms comprising cell membranes that contain ergosterol, e.g., fungi or parasites. Said compounds can be obtained using a method that consists in cultivating a producing micro-organism under conditions that enable the production thereof. In addition, the invention also relates to a mechanism for the in vitro production of amidated polyenes, consisting in incubating carboxylated polyenes with cell-free extracts (or proteinaceous fractions) of the producers of same in the presence of ATP/Mg⁺⁺ and an amide- group donor compound (preferably glutamine).

The invention discloses a technological process for extracting ergosterol with high purity and a feed protein from penicillin waste residue. According to the invention, mycelium in penicillin waste residue is taken as a production raw material, the invention comprises the following steps of distilling, concentrating, concreting, saponifying, extracting and drying and the like for extracting ergosterol and the feed protein. The method has the advantages of simple extracting method with high efficiency and low cost, the extracted products have the characteristics of high purity and good stability, which is suitable for industrial production.

The invention relates to a preparation method to produce ergosterol with yeast in fine chemical industry, which comprises: mixing the yeast, base and sodium chloride solution for saponification, extracting by toluene, cleaning with water, condensing, refining, and drying in vacuum. This method has convenient process with low cost and provides material guarantee for vitamin D2.