18 results about "Ergosterol" patented technology

The present disclosure provides a method for preparing vitamin D2 using a microchannel reactor, including: (a) dissolving ergosterol in a first solvent to form a first reaction solution; (b) introducing the first reaction solution into the microchannel reactor, and the first reaction solution is irradiated with light source A and light source B in sequence as the first reaction solution passes through a first photoreaction zone and a second photoreaction zone; (c) collecting and concentrating the first reaction solution irradiated by the light source B; (d) optionally, mixing the first reaction solution of step (c) with a second solvent to form a second reaction solution; (e) heating the first reaction solution of step (c) or the second reaction solution of step (d) to obtain the vitamin D2.

The invention discloses a method for converting ergosterol into vitamin D2 by using DES and a method for enriching vitamins. The method for converting the ergosterol into the vitamin D2 comprises the following steps: obtaining an ergosterol solution by taking a deep eutectic solvent as a solvent, and then placing the ergosterol solution under UVB ultraviolet rays to carry out light conversion, so that the ergosterol is converted into the vitamin D2. The method can improve the conversion rate and stability of ergosterol. The method for enriching vitamins comprises the following steps: (1) obtaining mushroom powder; (2) carrying out ultrasonic-assisted extraction on the mushroom powder by using a natural hydrophobic deep-eutectic solvent, and after the extraction is finished, centrifuging and taking supernate, so as to obtain an ergosterol extracting solution; and (3) placing the ergosterol extracting solution under UVB ultraviolet rays to carry out light conversion so as to obtain a solution rich in vitamin D2. According to the method, the extraction efficiency of ergosterol and the conversion rate and stability of vitamins are remarkably improved.

The invention relates to the technical field of biology, in particular to construction and application of a recombinant strain for synthesizing veratrazine and steroid precursors of the veratrazine, and the steroid precursors comprise squalene, ergosterol, lanosterol, 22R-hydroxy cholesterol, 22R, 26-dihydroxy cholesterol and 22-hydroxy-26-amino-cholesterol. The recombinant strain provided by the invention can efficiently express steroid precursors, for example, the content of lanosterol reaches 172mg / L and is increased by about 170 times compared with that of an original strain, the yield of 22R-hydroxycholesterol reaches 3482.83 mu g / L, the yield of 22R, 26-dihydroxycholesterol reaches 1003.05 mu g / L, veratrazine can be efficiently synthesized, and the yield of veratrazine reaches 265.38 mu g / L.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The invention discloses a method for preparing a laying hen feed additive by fermenting tea-seed oil deodorized distillate, which is characterized in that the tea-seed oil deodorized distillate is used as a raw material, and efficient utilization of all components of resources is achieved by coupling yeast-paecilomyces cicadae two-step sequential fermentation and endogenous mevalonic acid (MVA) pathway cascade regulation and control technology. The final product is rich in ergosterol, vitamin E and probiotics, can improve the laying rate of laying hens and enhance the eggshell strength after being applied to laying hen breeding, realizes zero wastewater discharge in the process, and achieves the dual goals of resource utilization of grease byproducts and accurate matching of laying hen nutrition.

The invention relates to the field of synthesis of organic fluorescent dyes, and discloses a conjugate of indocyanine green and cholesterol as well as synthesis and application of the conjugate. The conjugate is formed by connecting an indocyanine green derivative and a cholesterol derivative through an amido bond; the indocyanine green derivative is ICG-R-COOH; iCG is indocyanine green, R is-(CH2) nH. [I-], n is an integer of 1-6, or R is-(CH2) mSO3-, and m is 3 or 4; and the cholesterol derivative is cholesterole-NH2, beta-sitosterole-NH2, stigmasterole-NH2 or ergosterole-NH2. The invention also discloses a preparation method of the cholesterol derivative. The lipidosome constructed by the conjugate is high in stability, good in dispersity and strong in NIR-II fluorescence, the blood vessel and tumor imaging contrast ratio and definition under a 1300 nm near-infrared imaging window are superior to those of lipidosome constructed by commercial ICG, more excellent diagnosis and treatment performance is shown, and wide application prospects are achieved.

The invention provides a method for detecting the content of ergosterol in lentinus edodes based on near infrared spectroscopy and application. And rapidly detecting the content of the ergosterol in the shiitake mushrooms by adopting a near infrared spectrum technology, and inputting spectral data of a sample to be detected into the optimal model to obtain a predicted value of the ergosterol, so that the prediction of the content of the ergosterol in the shiitake mushrooms is realized. According to the method, the SG smoothing algorithm and the normalization algorithm are combined to preprocess the spectrum, noise generated when the sensor obtains the spectrum data can be effectively removed, and baseline and scattering correction is carried out; the variable combined population analysis-genetic algorithm is used for carrying out characteristic wavelength extraction on an original spectrum, so that required important variables can be effectively extracted, redundant information is efficiently removed, and the operation rate of the model is improved; the content of ergosterol in shiitake mushrooms is predicted by adopting the least square support vector machine model based on the crown porcupine optimization algorithm, and the model is high in adaptive capacity and high in prediction accuracy. And a new technical approach is provided for rapidly detecting the content of ergosterol.

The present application belongs to the technical field of edible and medicinal mushroom detection, and particularly relates to a characteristic spectrum construction method of deer mushroom extract and a method for determining the content of a marker effective component. The method is detected by high performance liquid chromatography, and the characteristic spectrum and the content of the effective component ergothioneine can be determined and analyzed by one chromatographic condition. The method comprises the following steps: taking formic acid acetonitrile solution as mobile phase A, taking formic acid solution as mobile phase B, performing gradient elution through a specific elution program, obtaining 9 characteristic peaks of ergosterol and ergothioneine, and quantitatively detecting the effective component ergothioneine by an external standard method. The method is simple in operation, short in detection time, and has good specificity, repeatability, precision and stability, can fully reflect the integrity and characteristics of deer mushroom and its extract, is suitable for the characterization and quality evaluation of the whole components of deer mushroom, and provides technical support and scientific basis for the full development and utilization of deer mushroom resources.

This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.

The present invention relates to the field of pharmaceutical chemistry, and specifically to a method for preparing cholesterol, a derivative thereof, and an analog thereof. The derivative of cholesterol comprises, but is not limited to, 7-dehydrocholesterol, 25-hydroxycholesterol, 25-hydroxy-7-dehydrocholesterol, and ergosterol. In the present invention, a compound represented by formula I can be prepared by means of a microbial transformation using phytosterols as a raw material, followed by preparing cholesterol, the derivative thereof, and the analog thereof.

The invention belongs to the technical field of detection of edible and medicinal fungi, and particularly relates to a characteristic spectrum construction method of a pilose antler mushroom extract and a method for determining the content of symbolic effective components. According to the method, high performance liquid chromatography is adopted for detection, and determination and analysis of the specific chromatogram and the content of the effective component ergothioneine can be achieved through one chromatographic condition. Comprising the steps that a formic acid acetonitrile solution serves as a mobile phase A, a formic acid solution serves as a mobile phase B, gradient elution is conducted through a specific elution procedure, nine characteristic peaks such as ergosterol and ergothioneine are obtained, the effective component ergothioneine is quantitatively detected through an external standard method, and the method is easy to operate and short in detection time and has good specificity, repeatability, precision and stability; the method can fully reflect the integrity and characteristics of the pilose antler mushroom and the extract thereof, is suitable for integral component characterization and quality evaluation of the pilose antler mushroom, and provides technical support and scientific basis for full development and utilization of pilose antler mushroom resources.

This invention discloses an HPLC method for simultaneously determining the content of six active ingredients in Hericium erinaceus extract, belonging to the field of natural compound detection and analysis technology. The detection steps of this invention include: preparing a mixed standard solution using Hericium erinaceus ketone E, Hericium erinaceus ketone C, Hericium erinaceus ketone D, Hericium erinaceus D, ergosterol, and Hericium erinaceus C as reference standards; analyzing the mixed standard solution using high-performance liquid chromatography (HPLC); plotting standard curves for the six active ingredients with the concentration of the mixed standard solution as the abscissa and the peak area as the ordinate; determining the linear regression equation; and calculating the content of each active ingredient in the sample solution. The HPLC method for simultaneously determining six active ingredients in Hericium erinaceus extract established in this invention has good selectivity, high sensitivity, accuracy, and reliability, providing a new analytical method and basis for the quality analysis and quality control of Hericium erinaceus extract.

The invention discloses a brewing method of Maotai-flavor morchella esculenta and highland barley health wine, and relates to the technical field of wine brewing, and the brewing method comprises the following steps: S1, preparing morchella esculenta strains; s2, pretreating and compounding the raw materials; s3, cooking and cooling; s4, performing yeast inoculation and aerobic diastatic fermentation; s5, morchella mycelium pellets are inoculated and subjected to solid anaerobic fermentation; s6, distilling and adding auxiliary materials; s7, wine mixing and precision control; s8, ageing and stabilizing treatment; and S9, finished product detection and filling. Through directional culture of morchella mycelium pellets, a large number of mycelium pellets uniform in size and high in activity are obtained through shaking culture of a shaking table, and then the mycelium pellets are crushed through high-speed homogenate, so that active ingredients such as polysaccharide and ergosterol in the mycelium pellets are fully released; the technologies of low-temperature aging, membrane filtration and the like are adopted in the brewing process, decomposition and loss of active ingredients are reduced to the maximum extent, the health preserving function is remarkable, and the highland barley wine is endowed with more nutritional values and health care functions on the basis that the original flavor of the highland barley wine is reserved.

The invention relates to the technical field of microbial fermentation, and discloses a tremella aurantialba functional fermentation metabolite directional enrichment process which comprises the following steps: S1, tremella aurantialba strain activation, S2, seed solution preparation, S3, controllable fermentation culture, S4, metabolite enrichment regulation and S5, separation and purification. According to the method, the fermentation temperature, the pH value, the dissolved oxygen content and the carbon nitrogen ratio are synergistically regulated and controlled, a metabolite synthesis inducer is added in a specific stage, a synthesis channel of polysaccharide and ergosterol in tremella aurantialba mycelia can be accurately activated, the polysaccharide content in the finally obtained mycelia is larger than 15% (on the basis of dry weight), and the polysaccharide content is larger than 15% (on the basis of dry weight). The content of ergosterol is more than 0.5% (on the basis of dry weight) and is increased by more than three to fifty percent compared with a traditional fermentation process, natural limitation of metabolite synthesis is effectively broken through, an automatic fermentation tank control system is adopted, parameters such as fermentation temperature, pH value and dissolved oxygen are regulated in real time, and the method is remarkably superior to a traditional manual regulation process. And the product quality consistency is ensured.

The invention discloses saccharomyces cerevisiae as well as related products and application thereof, and relates to the field of microorganisms. The saccharomyces cerevisiae BC-NHU-003 with excellent performance is obtained by separation and mutagenesis from Shaoxing rice wine distiller's yeast, has the characteristics of high growth speed and high yield of ergosterol, and provides a high-quality strain with high adaptability and high yield for industrial production of ergosterol.

The invention discloses a preparation device of ergosterol in a functional beverage, and belongs to the technical field of ergosterol extraction.The preparation device comprises a supporting column with the top provided with a tank body; and the crushing mechanism is arranged in the tank body and comprises a stirring part, an ultrasonic crushing generator and a microwave heating module. According to the preparation device for ergosterol in the functional beverage, through the use of the crushing mechanism, efficient synergistic extraction can be achieved, the efficiency and the yield are greatly improved, an ultrasonic crushing generator and a microwave heating module are integrated in the same reaction tank, the synergistic effect of physical crushing and heat energy extraction is achieved, and the extraction efficiency is improved. Ultrasonic waves utilize a cavitation effect to instantly destroy cell walls and release contents, and microwaves perform rapid and uniform internal heating and greatly accelerate mass transfer rate, so that the extraction process of ergosterol is shortened to several minutes from several hours traditionally, the extraction efficiency is high, the time is short, meanwhile, degradation of active ingredients caused by long-time heating is avoided, and the yield is higher.

Disclosed is use of reishi spore oil in the preparation of a drug for preventing and treating nodular diseases. Animal experiments show that reishi spore oil has the effect of significantly inhibiting the formation and development of benign granulomatous nodules in the lungs of mice, and also possesses good safety. The reishi spore oil can be used for preparing a drug for preventing and treating the formation and development of pulmonary nodules. The reishi spore oil comprises one or more components of triglyceride, ergosterol, and vitamin E; and the reishi polysaccharide ingredient is not detected.