529 results about "Sterol" patented technology

To provide a technique for enhancing the dermal delivery of a composition and, furthermore, enhancing the delivery of the composition to macrophage-surrounding areas.SOLUTION: The present invention provides a liposome with a particle diameter of 300 nm or less, comprising (A) polyglycerin fatty acid ester, (B) phospholipid, and (C) sterol. In a preferred embodiment, the liposome further comprises (D) an amphiphilic molecule having a disaccharide-to-trisaccharide hydrophilic group and an alkyl-chain hydrophobic group within its molecular structure.SELECTED DRAWING: None

Disclosed are compositions of lipid nanoparticles (LNP) comprising an ionizable cationic lipid, a phospholipid, a sterol, and a PEG-lipid (non-functionalized and optionally functionalized). The functionalized PEG-lipid can be conjugated with a binding moiety to create a targeted LNP (tLNP). The disclosed tLNP preferentially deliver a nucleic acid molecule or other negatively charged payload to cells expressing a cell surface antigen recognized by the binding moiety of the tLNP, and are better tolerated, as compared to LNPs and tLNPs comprising ionizable cationic lipids found in marketed pharmaceuticals comprising LNPs.

Compositions and methods for delivering nucleic acids (e.g. DNA) to cells are described herein. In some embodiments, the compositions and methods involve the use of lipid nanoparticles comprising cationic lipids, helper lipids, conjugated (PEG) lipids and structural (sterol) lipids wherein the helper lipid has at least one acyl chain bearing an unsaturation which improves in vivo delivery of the nucleic acid pay load.

The invention provides a liposome preparation and a preparation method thereof, the liposome preparation comprises an ion pairing agent, phospholipid or a derivative thereof, sterol, a lipid molecule derivative and a drug compound, the ion pairing agent comprises at least one of a compound (I) and a compound (II), and the compound (II) comprises at least one of a compound (III) and a compound (III). Structures in the compound (I) and the compound (II) contain a plurality of functional groups (such as amide, carbonyl, thioether and the like), so that abundant binding sites are provided for drug molecules, and the compound (I) and the compound (II) can interact with different types of drug compounds (such as fat-soluble and water-soluble drugs); the liposome preparation is allowed to be combined with various drug molecules through hydrogen bonds, hydrophobic interaction or electrostatic interaction, and the liposome preparation adapts to physicochemical properties of different drugs. The compound (I) and the compound (II) further comprise different hydrophobic groups (such as alkyl chains) and hydrophilic groups (such as sulfonic acid, carboxylic acid and the like), so that the liposome preparation has the amphiphilic characteristic, not only can encapsulate hydrophobic drugs, but also can encapsulate hydrophilic drugs, and the variety and range of encapsulated drugs are improved.

Disclosed are asphalt binder compositions and methods for making such compositions with pure sterol:crude sterol blends. The sterol blends improve various rheological properties.

Pavement aging can be reduced by applying to an asphalt-containing pavement a topcoat layer or a surface treatment containing asphalt binder emulsion with sterols.

The invention relates to the field of food processing, and discloses a processing method for debitterizing and deodorizing linseed oil and efficiently retaining antioxidant nutrient linol and grease processed thereof.The method comprises the steps that 1, the linseed oil and a debitterizing agent are mixed to obtain debitterized linseed oil; (2) sequentially carrying out steam quenching and tempering treatment and deodorization treatment on the linseed oil to obtain deodorized linseed oil; wherein the temperature of the deodorization treatment is 45-100 DEG C higher than that of the water vapor quenching and tempering treatment. According to the method, bitter and fishy substances in the linseed oil can be efficiently removed, meanwhile, the loss of alpha-linolenic acid, linolenol (containing tocopherol, PC-8 and polyphenol) and sterol in the linseed oil is reduced, the retention rate of the alpha-linolenic acid, linolenol and sterol in the linseed oil is increased, and then the quality of the linseed oil is improved.

Pavement aging can be reduced by applying to an asphalt-containing pavement a topcoat layer or a surface treatment containing asphalt binder with sterols.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol%; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol% and 65 mol%; and an ionizable cationic amino lipid present at a content of between 15 mol% and 45 mol%; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol% content is relative to total lipid present in the lipid nanoparticle.

The invention relates to the technical field of diglyceride oil, and discloses a diglyceride-containing grease composition and a preparation method thereof. According to the present invention, a variety of vegetable oils are combined, the combined vegetable oil is subjected to glycerol diesterification, and the turmeric is directly extracted by using the oil after the enzymolysis so as to integrally prepare the curcumin-containing diglyceride oil; according to the present invention, the mass content of diglyceride in the diglyceride oil prepared by combining a plurality of vegetable oils is 40-95%, the mass content of oryzanol is 10000-20000 ppm, the mass content of sterol is 8000-10000 ppm, and the mass content of curcumin is 0.1-0.2%, such that the diglyceride, the oryzanol, the sterol and the curcumin in the diglyceride oil are subjected to a specific content and proportion so as to prevent the color reversion of the oil composition, such that the color reversion of the oil composition can be prevented, and the oil composition can be easily obtained. The grease composition keeps bright appearance and has the advantage of being good in stability.

The present invention relates to a rapid testing method and a rapid testing kit for the sterol content of plant oils and fats. Rapid testing method: providing a sterol extraction solution of a plant oil and fat sample, diluting the sterol extraction solution with a buffer solution to obtain a sample solution, adding the sample solution to a sterol test plate, adding sterol oxidase for oscillation reaction, and obtaining the sterol content of the plant oil and fat based on the color depth of the solution of the sterol test plate being positively correlated with the sterol content. The method of the present invention is simple to operate, has a fast testing speed, and is low in cost. It is suitable for the rapid detection of the sterol content in plant oils and fats, and provides technical support for the screening of high sterol materials, variety breeding, and quality control of raw materials and products of processing enterprises.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol %; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol % and 65 mol %; and an ionizable cationic amino lipid present at a content of between 15 mol % and 45 mol %; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol % content is relative to total lipid present in the lipid nanoparticle.

The present invention pertains to a liposome comprising cannabidiol (CBD), a phospholipid component and optionally a sterol component, wherein the liposome comprises more than one bilayer, to related mixtures of liposomes, processes for manufacturing,medicalusesandcompositions.

Non-liposome, non-micelle particles formed of a lipid, an additional adjuvant such as a TLR4 agonist, a sterol, and a saponin are provided. The particles are porous, cage-like nanoparticles, also referred to as nanocages, and are typically between about 30 nm and about 60 nm. In some embodiments, the nanocages include or are administered in combination with an antigen. The particles can increase immune responses and are particularly useful as adjuvants in vaccine applications and related methods of treatment. Preferred lipids, additional adjuvants including TLR4 agonists, sterols, and saponins, methods of making the nanocages, and method of using them are also provided.

The present invention relates to compositions for in vitro and in vivo delivery of nucleic acids, in particular messenger RNAs (mRNAs), into a target cell and their applications. The present invention is directed to a composition comprising (A) at least one nucleic acid and (B) at least one lipid nanoparticle (LNP) comprising (i) at least one ionizable lipid; (ii) at least one phospholipid; (iii) at least one sterol, especially neutral sterol; (iv) at least one poly(ethyleneglycol)-lipid (PEG-lipid); and (v) an imidazolium-based cationic lipid of formula (I), wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9, Y and A− are as defined in the description. The present invention also relates to a method for in vitro or in vivo transfection of live cells and uses of said composition.

The present invention relates to a bioactive composition, with an antitumor effect, obtained from the transformation by alkylation and esterification reaction of an extract of the «Stipa tenacissima L» esparto plant. Said composition comprises at least one lupeol and / or β-sitosterol derivative selected from formulae (III) to (VI) obtained by alkylation or esterification reaction with a very high cytotoxicity efficiency.

The invention provides a rice bran fatty alkanol composition for reducing fat and losing weight and a preparation method of the rice bran fatty alkanol composition. The rice bran fatty alkanol composition for reducing fat and losing weight is composed of rice bran fatty alkanol and soybean sterol, and the rice bran fatty alkanol accounts for 5-30% of the mass of the composition. By optimizing the fingerprint spectrum of rice bran fatty alkanol, the composition proportion of higher fatty alkanol is effectively improved, the lipid-lowering, weight-losing and liver-protecting effects can be effectively improved, the lipid-lowering, weight-losing and liver-protecting effects are greatly improved by matching with soybean sterol, and a tablet containing the composition is further developed. The obtained lipid-lowering weight-losing liver-protecting tablet is safe and free of side effects, and can be eaten for a long time to prevent obesity and fatty liver.

Provided herein are lipid nanoparticle (LNP) compositions (e.g., pharmaceutical compositions) comprising a therapeutic nucleic acid (TNA), wherein the LNP comprises an ionizable lipid; a "helper" lipid, e.g., a ceramide or distearoylphosphatidylcholine (DSPC); a structural lipid, e.g., a sterol; and one or more types of lipid-anchored polymers, as well as uses thereof.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention discloses a skin-targeted layered infiltration polypeptide modified liposome and a preparation method thereof, and relates to the technical field of skin delivery and infiltration. The preparation method comprises the following steps: S1, mixing phospholipid, cell-penetrating permeation-enhancing peptide, sterol and a fat-soluble active component to prepare a lipid organic solution containing polypeptide, and mixing a water-soluble active component with distilled water to prepare a water-phase solution; s2, assembling a liposome precursor solution by adopting the micro-nano fluidic chip, inputting the lipid organic solution from the lipid phase channel inlet, inputting the water phase solution from the water phase channel inlet, and assembling the lipid organic solution and the water phase solution in the micro-nano fluidic chip to obtain the liposome precursor solution; and S3, separating the free drug from the organic solution in the liposome precursor solution to obtain the polypeptide modified liposome with targeted skin layered infiltration.

The present invention discloses a method for constructing a fingerprint spectrum of Cordyceps sinensis and its application. The method includes preparation of a test solution, preparation of a reference solution, preparation of a reference solution and high performance liquid chromatography detection, and the formulation of a fingerprint spectrum of Cordyceps sinensis sterol components. The liquid chromatography conditions of the present invention can effectively separate and detect the sterol components in Cordyceps sinensis, and the separation effect is good. This is the first HPLC fingerprint spectrum detection and identification method for sterol components in Cordyceps sinensis; the method provides favorable data support for the authenticity identification and / or quality control of Cordyceps sinensis and its products.

The present invention relates to a method for stabilizing ceramide and to a cosmetic composition containing stabilized ceramide and, more specifically, to a method for homogenizing and stabilizing ceramide by self-assembling the ceramide together with β-sitosterol, resveratrol, and allantoin which are other physiologically active substances using a microfluidic chip and a high pressure homogenizer, and to a technique for using ceramide and nanoparticles containing β-sitosterol, resveratrol, and allantoin, which are produced by means of the stabilizing method, as a cosmetic material for skin moisturizing, anti-oxidation, skin inflammation relief, skin barrier enhancement, and skin itching relief.

Disclosed are methods of treating cystic fibrosis using AmB and a sterol or compositions comprising AmB and a sterol. Also disclosed are methods of increasing the pH of airway surface liquid in a patient having cystic fibrosis using AmB and a sterol; and methods of decreasing the viscosity of airway surface liquid in a patient having cystic fibrosis using AmB and a sterol.

The invention belongs to the field of biological medicine, and particularly relates to lipid nanoparticles, application thereof and a drug delivery carrier. The lipid nanoparticle comprises an ionizable lipid, cholesterol, phospholipid, a pegylated lipid and a sterol substitute, and is characterized in that the ionizable lipid is Lipid 5, the phospholipid is DSPC, the pegylated lipid is 18: 0 mPEG2000 PE, and the sterol substitute is one or more of beta-sitosterol, stigmasterol or stigmasterol or a derivative thereof; the molar ratio of the ionizable lipid to the cholesterol to the phospholipid to the pegylated lipid to the sterol substitute is (40-60): (5-38.5): (5-30): (1-2): (5-38.5). According to the invention, the LNP with unique physicochemical properties is constructed through high synergy of the components, the technical problems of low delivery efficiency and poor specificity of hematopoietic stem progenitor cells in the prior art are solved, and high-efficiency and high-specificity drug delivery of HSPCs in vivo and in vitro is realized. The invention also provides a drug delivery carrier containing the lipid nanoparticles and a preparation method thereof.

The invention relates to the field of food processing, and discloses a flavor oil processing technology for improving phenolic nutrient substances, which comprises the following steps: (1) mixing oil seeds with water, and carrying out low-temperature treatment at 20 DEG C or below to obtain wetted oil seeds; (2) stir-frying and squeezing the wetted oil seeds to obtain first crude oil and an oil cake; (3) mixing the oil cake with water, performing heat treatment, and performing oil extraction to obtain second crude oil; and (4) mixing the first crude oil and the second crude oil to obtain the vegetable oil. By adopting the method disclosed by the invention, the content of polyphenol, sterol and flavor substances in the vegetable oil can be further improved.

The present invention provides, in part, sterol-based cationic lipids with aromatic head groups of Formula (I), and sub-formulas thereof: (I), or a pharmaceutically acceptable salt thereof. The compounds provided herein can be useful for delivery and expression of mRNA and encoded protein, e.g., as a component of liposomal delivery vehicle, and accordingly can be useful for treating various diseases, disorders and conditions, such as those associated with deficiency of one or more proteins.

The present invention relates to (1) a method for producing biochar having bio stimulating effects, (2) biochar that has been produced to having increased bio stimulating effects, (3) a method for capturing material extracted from biochar and (4) biochar extracts, comprising desired compounds extracted from biochar and suspended in an extract solution. The methods above all involve obtaining a particular biomass source and pyrolyzing the biomass source under specific conditions to increase the bio stimulating effects of the biochar. The method for capturing the material extracted from the biochar produced to have such stimulating effects includes contacting the biochar with a treating liquid, separating the biochar from the biochar extract, collecting the biochar extract from which the biochar has been separated and resuspending the biochar extract in an modified aqueous solution for further use and / or processing separate from, or prior to, disposal. The biochar extract suspended in solution then includes desired compounds (a) at least 0.1 ppm of one or more of the following phenylpropanoid, phenylalanine, flavonoids, sterols, terpenes, terpenoids, tannins, coumarins, benzaldehydes and phenols; (b) at least 0.1 g / L of humics, fulvics or a combination of both humics and fulvics and / or (c) one or more of the following salicylic acid, jasmonic acid, abscisic acid, cytokinin, gibberellic acid and ethylene, by way of example.

Provided herein are compositions including ionizable lipids and lipid nanoparticles comprising the ionizable lipids. The ionizable lipids may have a general structure according to formula I: (I). Lipid nanoparticles generally include the ionizable lipid according to formula (I); a helper lipid; a sterol; and a PEGylated lipid conjugate. The ionizable lipids and lipid nanoparticles may be used to carrier cargo for a vaccine.

Disclosed are methods of treating cystic fibrosis using AmB and a sterol or compositions comprising AmB and a sterol. Also disclosed are methods of increasing the pH of airway surface liquid in a patient having cystic fibrosis using AmB and a sterol; and methods of decreasing the viscosity of airway surface liquid in a patient having cystic fibrosis using AmB and a sterol.