323 results about "Sterol" patented technology

Disclosed are compositions of lipid nanoparticles (LNP) comprising an ionizable cationic lipid, a phospholipid, a sterol, and a PEG-lipid (non-functionalized and optionally functionalized). The functionalized PEG-lipid can be conjugated with a binding moiety to create a targeted LNP (tLNP). The disclosed tLNP preferentially deliver a nucleic acid molecule or other negatively charged payload to cells expressing a cell surface antigen recognized by the binding moiety of the tLNP, and are better tolerated, as compared to LNPs and tLNPs comprising ionizable cationic lipids found in marketed pharmaceuticals comprising LNPs.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol %; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol % and 65 mol %; and an ionizable cationic amino lipid present at a content of between 15 mol % and 45 mol %; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol % content is relative to total lipid present in the lipid nanoparticle.

The present invention pertains to a liposome comprising cannabidiol (CBD), a phospholipid component and optionally a sterol component, wherein the liposome comprises more than one bilayer, to related mixtures of liposomes, processes for manufacturing,medicalusesandcompositions.

The present invention relates to compositions for in vitro and in vivo delivery of nucleic acids, in particular messenger RNAs (mRNAs), into a target cell and their applications. The present invention is directed to a composition comprising (A) at least one nucleic acid and (B) at least one lipid nanoparticle (LNP) comprising (i) at least one ionizable lipid; (ii) at least one phospholipid; (iii) at least one sterol, especially neutral sterol; (iv) at least one poly(ethyleneglycol)-lipid (PEG-lipid); and (v) an imidazolium-based cationic lipid of formula (I), wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9, Y and A− are as defined in the description. The present invention also relates to a method for in vitro or in vivo transfection of live cells and uses of said composition.

Provided herein are lipid nanoparticle (LNP) compositions (e.g., pharmaceutical compositions) comprising a therapeutic nucleic acid (TNA), wherein the LNP comprises an ionizable lipid; a "helper" lipid, e.g., a ceramide or distearoylphosphatidylcholine (DSPC); a structural lipid, e.g., a sterol; and one or more types of lipid-anchored polymers, as well as uses thereof.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention discloses a skin-targeted layered infiltration polypeptide modified liposome and a preparation method thereof, and relates to the technical field of skin delivery and infiltration. The preparation method comprises the following steps: S1, mixing phospholipid, cell-penetrating permeation-enhancing peptide, sterol and a fat-soluble active component to prepare a lipid organic solution containing polypeptide, and mixing a water-soluble active component with distilled water to prepare a water-phase solution; s2, assembling a liposome precursor solution by adopting the micro-nano fluidic chip, inputting the lipid organic solution from the lipid phase channel inlet, inputting the water phase solution from the water phase channel inlet, and assembling the lipid organic solution and the water phase solution in the micro-nano fluidic chip to obtain the liposome precursor solution; and S3, separating the free drug from the organic solution in the liposome precursor solution to obtain the polypeptide modified liposome with targeted skin layered infiltration.

The invention belongs to the field of biological medicine, and particularly relates to lipid nanoparticles, application thereof and a drug delivery carrier. The lipid nanoparticle comprises an ionizable lipid, cholesterol, phospholipid, a pegylated lipid and a sterol substitute, and is characterized in that the ionizable lipid is Lipid 5, the phospholipid is DSPC, the pegylated lipid is 18: 0 mPEG2000 PE, and the sterol substitute is one or more of beta-sitosterol, stigmasterol or stigmasterol or a derivative thereof; the molar ratio of the ionizable lipid to the cholesterol to the phospholipid to the pegylated lipid to the sterol substitute is (40-60): (5-38.5): (5-30): (1-2): (5-38.5). According to the invention, the LNP with unique physicochemical properties is constructed through high synergy of the components, the technical problems of low delivery efficiency and poor specificity of hematopoietic stem progenitor cells in the prior art are solved, and high-efficiency and high-specificity drug delivery of HSPCs in vivo and in vitro is realized. The invention also provides a drug delivery carrier containing the lipid nanoparticles and a preparation method thereof.

Provided herein are compositions including ionizable lipids and lipid nanoparticles comprising the ionizable lipids. The ionizable lipids may have a general structure according to formula I: (I). Lipid nanoparticles generally include the ionizable lipid according to formula (I); a helper lipid; a sterol; and a PEGylated lipid conjugate. The ionizable lipids and lipid nanoparticles may be used to carrier cargo for a vaccine.

Insects with elevated levels of protein, chitin, elements, amino acids, vitamins, and / or sterols are disclosed. The insects have elevated levels due to a diet that includes mushroom biomass. The mushroom biomass includes any one or more of the mushroom fruitbody, the stem, and the mycelium. The enriched insects with biomolecules from mushroom biomass can be used as they are or as supplements to increase the levels of biomolecules in humans or other animals that may ingest the insects or a modified form of the insect.

The present invention discloses a natural firming, anti-wrinkle and brightening plant essential oil in the field of skin care products, its preparation method and application. The components of the essential oil include frankincense oil, sandalwood oil, lavender oil, myrrh oil, helichrysum italicum oil, cistus ladaniferus oil, rosa damascena flower oil, vetiver root oil, and marine biological sterols. The marine biological sterols are composed of a mixture of ascidiasterol and sarcophytol A. This plant essential oil can be applied in skin care products such as aqueous solutions, facial masks, essence, lotions, creams, and liquid foundations. The present invention adopts a unique essential oil formula, making the product outstanding in the comprehensive effect of anti-wrinkle and brightening, and without any chemical components, and all comply with the relevant regulations of the "Technical Specifications for Cosmetics Safety", with high safety. In the plant essential oil of the present invention, marine biological sterols made of ascidiasterol and sarcophytol A are added, which have the effects of antioxidant and moisturizing by themselves, and can also improve the penetration and absorption of the essential oil, thereby further enhancing the anti-wrinkle and brightening effects.

Provided herein are lipid nanoparticles encapsulating nucleic acids having a neutral lipid in a molar ratio of at least 30%, a sterol or a derivative thereof, and a targeting moiety anchored in its lipid layer by a lipophilic moiety. Methods of using the lipid nanoparticles for targeted delivery in vivo are also provided. Such lipid nanoparticles may have significantly improved delivery and targeting to extrahepatic tissues and / or organs.

The present disclosure provides compositions that exhibit preferential targeting or delivery of nucleic acid compositions to specific organs. In some embodiments, the compositions comprise steroids or sterols, ionizable cationic lipids, phospholipids, PEG lipids, and permanent cationic lipids, which can be used to deliver nucleic acids. The invention also provides the use of the composition in the preparation of a medicament for targeted delivery of a therapeutic agent to an organ or cells in an organ.

The invention discloses a sterol substance-amino acid lipid, preparation thereof and application of the sterol substance-amino acid lipid in a nano drug delivery system.The sterol substance-amino acid lipid is obtained by connecting a sterol substance and amino acid through an ester bond; then, the sterol substance-amino acid lipid, the auxiliary lipid and the PEGylated lipid jointly construct the nano drug delivery system, the prepared sterol substance-amino acid lipid can directly replace cholesterol in traditional LNP components, the limitation of traditional four-component mode LNP particles is overcome, and the stability of the nano drug delivery system is improved. Meanwhile, the drug delivery system prepared from the sterol substance-amino acid lipid has high encapsulation efficiency, high transfection efficiency and low biotoxicity.

The invention discloses a preparation for promoting efficient yield increase and tree cultivation of rubber trees. The invention belongs to the technical field of rubber tree planting, and the preparation comprises CMC-Na, Yitenling, a Nobawen chelating nutrient solution, boron hydroxide, sodium benzoate, melissyl alcohol, 14-sterol and other components, the concentration of each component is optimally designed, and the components are uniformly prepared through special stirring equipment; when in use, the preparation is smeared on a secant of a rubber tree, the latex yield can be remarkably increased, the dead skin rate is reduced, and the dry glue content is increased by continuous use; the problems that the yield increasing effect of rubber trees is limited and maintenance is insufficient are solved through scientific matching and synergistic effect, the method has the advantages of being wide in raw material source, controllable in cost and easy and convenient to operate, is suitable for large-scale popularization, and provides powerful support for sustainable development of the natural rubber industry.

The disclosure provides nanoparticle lipid compositions, methods of manufacturing, and uses thereof. In one aspect, a lipid nanoparticle composition includes: (a) an ionizable lipid; (b) a sterol; and (c) a stabilizer. The disclosure provides methods for preparing the lipid nanoparticle compositions and uses of the lipid nanoparticle or the pharmaceutical composition for preventing, treating, or ameliorating conditions or diseases.

This application relates to the field of cosmetics, and specifically discloses a composition with a skin barrier repair function and its applications. The composition with a skin barrier repair function comprises the following components in parts by weight: 20-40 parts of functional microcapsules; 10-15 parts of Isatis indigotica extract; 3-5 parts of hydrolyzed Abelmoschus esculentus extract; the core material of the functional microcapsules comprises ceramide, vegetable oil and sterol, and the weight ratio of ceramide, vegetable oil and sterol is 1:(1.5-3):(0.8-1.5). The composition of the present application can be used to prepare cosmetics, and has the advantages of better soothing and repairing the skin.

Lipid nanoparticles for delivery of nucleic acid molecules, such as mRNA, are provided. Methods of making and using the same are also provided.

The present disclosure provides compositions which facilitate preferential targeting or delivery of a therapeutic agent to a particular organ, cell, or tissue. The presently disclosed compositions comprise lipid nanoparticles formed from a covalent-bond forming lipid, a cationic lipid, and, in some embodiments, a steroid or sterol, a phospholipid, and a PEG lipid.

The present invention relates to an extract containing Myconoside, which is derived from an in vitro system of a plant of the family Gesneraceae, in particular from the genus Haberlea and Ramonda, including species Haberlea rhodopsis Friv., Ramonda helichii (Bois.C. B. Clarke, Ramonda myconi (L.) Rchb., Ramonda nathaliae Pancicamp, and a method for preparing the extract containing Myconoside. The extract containing Myconoside, which is derived from an in vitro system of a plant of the family Gesneraceae, in particular from the genus Haberlea and Ramonda, includes the species of Haberlea rhodopsis Friv. The invention relates to an extract, in particular to petrovic, Ramonda serbic Panic, Petrovic and Ramonda serbic Panic, as well as hybrids thereof, and a preparation method of the extract. Furthermore, the invention relates to the use of said extract as a chemical, radiation and UV protection agent in human and veterinary medicine, in nutritional supplements and in cosmetics. The plant in-vitro system extract is used independently or in a mixed mode, and comprises a polyphenol component extract 1 mainly containing flavone-C glycoside and phenethyl alcohol compounds (PHE) and a Mycoside component extract 2 mainly containing phenethyl alcohol compounds (particularly Mycoside and Paucifloside). The extract 1 contains 0.1-55.0 wt% of PHE, free phenol, saccharides, organic acid, fatty acid, amino acid, sterol and the like, and the total amount is 100 wt%; the extract 2 contains 55.0 to 99.9 weight percent of PHE (particularly Myconoside and Paucifloside) and phenolic compounds, monosaccharides, disaccharides or residues thereof, and the total amount is 100 weight percent. These compounds, in particular Myconoside, have high biological activity, anti-oxidation, anti-mutation and anti-chromosome-disruption activity, and thus are suitable for use alone, also can be used in combination by metered mixing, or can be conditioned by dilution, for the preparation of natural chemical, radio and ultraviolet protective agents with a controllable degree of protection. The latter is used in humans and veterinary, nutritional supplements and cosmetics to prevent and treat harmful chemical, radiation and UV effects.

The invention discloses an acute kidney injury nuclear magnetic resonance detection contrast agent as well as a preparation method and application thereof. The lipidosome comprises a first lipid component and a second lipid component, the first lipid component is any one of 1, 2-dipalmitoyl-sn-glycerol-3-phosphorylcholine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylcholine and distearoyl phosphatidylcholine, and the second lipid component is a sterol derivative, and the first lipid component is any one of 1, 2-dipalmitoyl-sn-glycerol-3-phosphorylcholine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylcholine and distearoyl phosphatidylcholine. The molar ratio of the first lipid component to the second lipid component is (1-3): 1. According to the invention, all organic free radicals are used for replacing gadolinium ions, so that renal toxicity and systemic fibrosis risks caused by metal accumulation are eliminated, and the method is especially suitable for imaging of patients with renal insufficiency. The material synthesis process is simple, and the cost is controllable. In addition, the system is seamlessly compatible with clinical 3.0 T MRI equipment, additional hardware transformation is not needed, and popularization and application of AKI bedside rapid imaging diagnosis can be directly promoted.

The invention provides a monascus strain, monascus, monascus decoction pieces, a preparation method of monascus, a monascus extract and a medicine. The Latin chemical name of the monascus purpureus strain is Monascus purpureus, and the preservation number of the monascus purpureus strain is CGMCC (China General Microbiological Culture Collection Center) No.40777. In a monascus product prepared by fermentation of the monascus strain provided by the invention, the content of lovastatin compounds and the content of extracts are high, and the monascus product contains polyhydroxy sterol and flavonoid components and is good in safety, so that the pharmaceutical activity and quality of the monascus product are improved.

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

The present invention relates to a standardized plant extract of biomass from an in vitro culture of Brassica granatum, containing biologically active compounds and their primary and secondary metabolites, comprising, in% by weight: 4.0 to 6.0 organic acids, 0.5 to 1.5 fatty acids, 8.0 to 12.0 amino acids, 0.5 to 1.0 sterols, 3.0 to 6.0 free phenols, 45 to 55 sugars and 25.0 to 35.0 polyphenols, wherein the content of major mannose glycosides in the polyphenol fraction is 70-96%, making up 18%-35% of the total extract, and to compositions containing the standardized extract and glycerol and to methods for preparing standardized plant extracts. According to the method of the invention, along with the optimally selected steps, specific conditions, parameters such as temperature, duration, agitation, light, growth factors and the like, maximum volume productivity of the target substance and mannoside and stable productivity of in vitro plant cultures are achieved, and the method is a reliable and effective 24 / 7 continuous system for the production of NP.

The present invention relates to a temperature-responsive in situ gelling composition comprising an amphiphilic triblock copolymer of polyoxyethylene-polyoxypropylene-polyoxyethylene (PEO-PPO-PEO), a water-soluble hydrophilic polymer, wherein the composition further comprises at least one lipid agent. The lipid agent is selected from the group consisting of a phospholipid, a sterol, a glyceride, a fatty acid or ester and derivative, a fatty alcohol, a cholesterol and derivative or a combination of more than one lipid agent. The present invention further relates to a vascular embolization agent comprising the temperature-responsive in situ gelling composition.

The purpose of the present invention is to provide: a W / O / W emulsion having good temporal stability; and a method for producing the same. The present invention relates to a W / O / W emulsion comprising an inner aqueous phase, an oil phase, and an outer aqueous phase, wherein a polyoxyethylene sterol ether is contained.

The present application relates to the technical field of biological medicine, and discloses a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia, after the ganoderma lucidum thick piece is treated by the processes of spraying moistening, hot pressure steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpene and sterol is improved, and the release rate of active ingredients is also significantly improved. The structure of the ganoderma lucidum mycelium cell wall is damaged by the present application technology, so that the active ingredients such as polysaccharide, triterpene and sterol originally existing in the ganoderma lucidum can be easily extracted and released. The ganoderma lucidum cells are loosened and broken in the present application, and the polysaccharide in the combined state can be changed into the free state, so that the content of the ganoderma lucidum polysaccharide can be greatly improved. The active ingredients in the ganoderma lucidum can be quickly and maximally released in the digestive tract by directly taking the broken wall superfine powder of the broken cells, so that the effect of the superfine powder on improving female insomnia is improved.

A lipid nanoparticle composition for use in the delivery of a self-amplifying RNA (saRNA) construct in one or more target cells is disclosed. The lipid nanoparticle composition comprises a lipid mixture comprising at least one (ionizable) cationic lipid, at least one helper lipid, a sterol, a corticosteroid such as Dexamethasone, and a least one lipid-polyethylene glycol conjugate. The saRNA construct comprises a first open reading frame which encodes one or more non-structural proteins, and a second open reading frame operatively linked to the first open reading frame. The second open reading frame comprises a coding region which encodes one or more target proteins. In some embodiments, the target proteins comprise one or more therapeutic proteins. In some embodiments, the target proteins comprise one or more one or more Cas proteins and / or variants thereof for use in CRISPR-based gene editing.