226 results about "Sterol" patented technology

The present invention relates to compositions for in vitro and in vivo delivery of nucleic acids, in particular messenger RNAs (mRNAs), into a target cell and their applications. The present invention is directed to a composition comprising (A) at least one nucleic acid and (B) at least one lipid nanoparticle (LNP) comprising (i) at least one ionizable lipid; (ii) at least one phospholipid; (iii) at least one sterol, especially neutral sterol; (iv) at least one poly(ethyleneglycol)-lipid (PEG-lipid); and (v) an imidazolium-based cationic lipid of formula (I), wherein R1, R2, R3, R4, R5, R6, R7, R8 and R9, Y and A− are as defined in the description. The present invention also relates to a method for in vitro or in vivo transfection of live cells and uses of said composition.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention discloses a skin-targeted layered infiltration polypeptide modified liposome and a preparation method thereof, and relates to the technical field of skin delivery and infiltration. The preparation method comprises the following steps: S1, mixing phospholipid, cell-penetrating permeation-enhancing peptide, sterol and a fat-soluble active component to prepare a lipid organic solution containing polypeptide, and mixing a water-soluble active component with distilled water to prepare a water-phase solution; s2, assembling a liposome precursor solution by adopting the micro-nano fluidic chip, inputting the lipid organic solution from the lipid phase channel inlet, inputting the water phase solution from the water phase channel inlet, and assembling the lipid organic solution and the water phase solution in the micro-nano fluidic chip to obtain the liposome precursor solution; and S3, separating the free drug from the organic solution in the liposome precursor solution to obtain the polypeptide modified liposome with targeted skin layered infiltration.

The invention belongs to the field of biological medicine, and particularly relates to lipid nanoparticles, application thereof and a drug delivery carrier. The lipid nanoparticle comprises an ionizable lipid, cholesterol, phospholipid, a pegylated lipid and a sterol substitute, and is characterized in that the ionizable lipid is Lipid 5, the phospholipid is DSPC, the pegylated lipid is 18: 0 mPEG2000 PE, and the sterol substitute is one or more of beta-sitosterol, stigmasterol or stigmasterol or a derivative thereof; the molar ratio of the ionizable lipid to the cholesterol to the phospholipid to the pegylated lipid to the sterol substitute is (40-60): (5-38.5): (5-30): (1-2): (5-38.5). According to the invention, the LNP with unique physicochemical properties is constructed through high synergy of the components, the technical problems of low delivery efficiency and poor specificity of hematopoietic stem progenitor cells in the prior art are solved, and high-efficiency and high-specificity drug delivery of HSPCs in vivo and in vitro is realized. The invention also provides a drug delivery carrier containing the lipid nanoparticles and a preparation method thereof.

Insects with elevated levels of protein, chitin, elements, amino acids, vitamins, and / or sterols are disclosed. The insects have elevated levels due to a diet that includes mushroom biomass. The mushroom biomass includes any one or more of the mushroom fruitbody, the stem, and the mycelium. The enriched insects with biomolecules from mushroom biomass can be used as they are or as supplements to increase the levels of biomolecules in humans or other animals that may ingest the insects or a modified form of the insect.

The invention discloses a preparation for promoting efficient yield increase and tree cultivation of rubber trees. The invention belongs to the technical field of rubber tree planting, and the preparation comprises CMC-Na, Yitenling, a Nobawen chelating nutrient solution, boron hydroxide, sodium benzoate, melissyl alcohol, 14-sterol and other components, the concentration of each component is optimally designed, and the components are uniformly prepared through special stirring equipment; when in use, the preparation is smeared on a secant of a rubber tree, the latex yield can be remarkably increased, the dead skin rate is reduced, and the dry glue content is increased by continuous use; the problems that the yield increasing effect of rubber trees is limited and maintenance is insufficient are solved through scientific matching and synergistic effect, the method has the advantages of being wide in raw material source, controllable in cost and easy and convenient to operate, is suitable for large-scale popularization, and provides powerful support for sustainable development of the natural rubber industry.

The disclosure provides nanoparticle lipid compositions, methods of manufacturing, and uses thereof. In one aspect, a lipid nanoparticle composition includes: (a) an ionizable lipid; (b) a sterol; and (c) a stabilizer. The disclosure provides methods for preparing the lipid nanoparticle compositions and uses of the lipid nanoparticle or the pharmaceutical composition for preventing, treating, or ameliorating conditions or diseases.

Lipid nanoparticles for delivery of nucleic acid molecules, such as mRNA, are provided. Methods of making and using the same are also provided.

The invention discloses an acute kidney injury nuclear magnetic resonance detection contrast agent as well as a preparation method and application thereof. The lipidosome comprises a first lipid component and a second lipid component, the first lipid component is any one of 1, 2-dipalmitoyl-sn-glycerol-3-phosphorylcholine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylcholine and distearoyl phosphatidylcholine, and the second lipid component is a sterol derivative, and the first lipid component is any one of 1, 2-dipalmitoyl-sn-glycerol-3-phosphorylcholine, 1, 2-dioleoyl-sn-glycerol-3-phosphorylcholine and distearoyl phosphatidylcholine. The molar ratio of the first lipid component to the second lipid component is (1-3): 1. According to the invention, all organic free radicals are used for replacing gadolinium ions, so that renal toxicity and systemic fibrosis risks caused by metal accumulation are eliminated, and the method is especially suitable for imaging of patients with renal insufficiency. The material synthesis process is simple, and the cost is controllable. In addition, the system is seamlessly compatible with clinical 3.0 T MRI equipment, additional hardware transformation is not needed, and popularization and application of AKI bedside rapid imaging diagnosis can be directly promoted.

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

The present invention relates to a standardized plant extract of biomass from an in vitro culture of Brassica granatum, containing biologically active compounds and their primary and secondary metabolites, comprising, in% by weight: 4.0 to 6.0 organic acids, 0.5 to 1.5 fatty acids, 8.0 to 12.0 amino acids, 0.5 to 1.0 sterols, 3.0 to 6.0 free phenols, 45 to 55 sugars and 25.0 to 35.0 polyphenols, wherein the content of major mannose glycosides in the polyphenol fraction is 70-96%, making up 18%-35% of the total extract, and to compositions containing the standardized extract and glycerol and to methods for preparing standardized plant extracts. According to the method of the invention, along with the optimally selected steps, specific conditions, parameters such as temperature, duration, agitation, light, growth factors and the like, maximum volume productivity of the target substance and mannoside and stable productivity of in vitro plant cultures are achieved, and the method is a reliable and effective 24 / 7 continuous system for the production of NP.

The present invention relates to a temperature-responsive in situ gelling composition comprising an amphiphilic triblock copolymer of polyoxyethylene-polyoxypropylene-polyoxyethylene (PEO-PPO-PEO), a water-soluble hydrophilic polymer, wherein the composition further comprises at least one lipid agent. The lipid agent is selected from the group consisting of a phospholipid, a sterol, a glyceride, a fatty acid or ester and derivative, a fatty alcohol, a cholesterol and derivative or a combination of more than one lipid agent. The present invention further relates to a vascular embolization agent comprising the temperature-responsive in situ gelling composition.

The purpose of the present invention is to provide: a W / O / W emulsion having good temporal stability; and a method for producing the same. The present invention relates to a W / O / W emulsion comprising an inner aqueous phase, an oil phase, and an outer aqueous phase, wherein a polyoxyethylene sterol ether is contained.

The present application relates to the technical field of biological medicine, and discloses a preparation method of ganoderma lucidum broken wall superfine powder for female insomnia, after the ganoderma lucidum thick piece is treated by the processes of spraying moistening, hot pressure steam heating, ultrasonic treatment and microwave drying, the content of polysaccharide, triterpene and sterol is improved, and the release rate of active ingredients is also significantly improved. The structure of the ganoderma lucidum mycelium cell wall is damaged by the present application technology, so that the active ingredients such as polysaccharide, triterpene and sterol originally existing in the ganoderma lucidum can be easily extracted and released. The ganoderma lucidum cells are loosened and broken in the present application, and the polysaccharide in the combined state can be changed into the free state, so that the content of the ganoderma lucidum polysaccharide can be greatly improved. The active ingredients in the ganoderma lucidum can be quickly and maximally released in the digestive tract by directly taking the broken wall superfine powder of the broken cells, so that the effect of the superfine powder on improving female insomnia is improved.

A lipid nanoparticle composition for use in the delivery of a self-amplifying RNA (saRNA) construct in one or more target cells is disclosed. The lipid nanoparticle composition comprises a lipid mixture comprising at least one (ionizable) cationic lipid, at least one helper lipid, a sterol, a corticosteroid such as Dexamethasone, and a least one lipid-polyethylene glycol conjugate. The saRNA construct comprises a first open reading frame which encodes one or more non-structural proteins, and a second open reading frame operatively linked to the first open reading frame. The second open reading frame comprises a coding region which encodes one or more target proteins. In some embodiments, the target proteins comprise one or more therapeutic proteins. In some embodiments, the target proteins comprise one or more one or more Cas proteins and / or variants thereof for use in CRISPR-based gene editing.

The present invention features new lipid-polymer composite particles that are useful for the formulation of bioactive agents for administration to a subject. The nanoparticles include a block copolymer, a lipid, e.g., phospholipid, and a sterol. The formulations of a bioactive agent (e.g., a therapeutic agent, a nutraceutical agent, or a recreational agent) described herein provide for easier loading of lipid-polymer composite particles with higher drug loading capacity, increased stability of the formulations, and lower surface tension of water, which allows for lipid coating and entrapment.

The invention discloses a novel multi-dimensional cascade assembled micro-nano intestinal delivery carrier and a preparation method and application thereof, and relates to the field of biological medicine and functional materials.The preparation method comprises the following steps that S1, phospholipid and a sterol regulator are dissolved in an ethanol solution to form a lipid film, the solution is added for hydration, and the lipid film is obtained; then treating to obtain a monodisperse vesicle suspension; s2, adding the vesicle suspension into the composite solution, and inducing adsorption of an inner shell layer to form intermediate particles; and S3, dropwise adding a cationic electrolyte solution into the intermediate particle suspension, and carrying out interface electrostatic complexing and coordination cross-linking reaction to obtain the delivery carrier. By constructing a cascade self-assembly delivery carrier with a multi-layer core-shell structure, the problem that a large amount of drugs are inactivated due to insufficient shielding in an extreme stomach environment can be solved, so that space-time limited release of the drugs in specific parts of intestinal tracts is realized, and oral bioavailability and targeted therapy efficiency are greatly improved.

A method for inducing an immune response in an individual to treat or prevent a disease or condition is provided, the method comprising administering a vaccine comprising lipid nanoparticles encapsulating nucleic acids encoding antigen proteins, peptides, or fragments thereof, a neutral lipid content of 30% to 70% molar ratio, sterols or derivatives thereof, and 5 to 50% molar ratio of ionizable cationic amino lipids, and a hydrophilic polymer-lipid conjugate present at a lipid content of 0% to 5% molar ratio, wherein administration of the lipid nanoparticles results in an immune response in an individual against the antigen protein, peptide, or fragment thereof expressed by mRNA, wherein each % molar ratio of lipid content relative to the total lipid content of the lipid nanoparticles is measured. This disclosure also provides vaccine compositions and the use of such compositions in inducing an immune response in an individual.

The application provides a method for identifying adulteration of tea oil based on C-4 dimethyl sterol composition, wherein the proportion of the peak area of C-4 dimethyl sterol in the total peak area in collected tea oil adulteration samples with different adulteration proportions is subjected to least square linear regression analysis with different adulteration proportions, a detection model for adulteration is established by combining with the fluorescence signal intensity of Filipin III, and whether other oil is adulterated in the tea oil sample is determined, and the adulteration proportion can be determined according to the ratio of the fluorescence signal intensity of Filipin III to the standard threshold value of tea oil. The method provided by the application can not only identify the adulteration of tea oil, but also detect the adulteration proportion, has the characteristics of simplicity, rapidness, good repeatability, high accuracy and the like, and can be practically applied to the detection of tea oil adulteration.

The present application aims to provide a new method, reagent and kit for measuring sterol in lipoprotein. By contacting lipoprotein in a test sample obtained from a subject, a labeled sterol having a label attached to the C3 position of the sterol skeleton, and a first capture body that specifically binds to the label and has a labeling substance, by forming a complex containing the lipoprotein containing the labeled sterol and the first capture body, detecting a signal generated from the labeling substance contained in the complex, the problem is solved.

The invention belongs to the field of biological medicine, and particularly relates to lipid nanoparticles for local drug delivery, a preparation method of the lipid nanoparticles and application of the lipid nanoparticles to preparation of anti-scar drugs. The lipid nanoparticle comprises a lipid carrier and nucleic acid wrapped by the lipid carrier, and the lipid carrier comprises the following components: an ionizable lipid, a permanent cationic lipid, a pegylated lipid, an auxiliary phospholipid and a sterol compound; the molar ratio of the ionizable lipid to the permanent cationic lipid to the pegylated lipid to the auxiliary phospholipid to the sterol compound is (25-45): (5-25): (1-2): (5-15): (35-40). In order to solve the problem of single administration of lipid nanoparticles in the prior art, the lipid nanoparticles are designed to be externally applied and applied for local administration through proportion regulation and control, so that the targeting property is improved, and the administration concentration is increased. Whole-body blood circulation is avoided, and toxic and side effects and off-target performance are reduced. Therefore, the dual purposes of synergism and toxicity reduction are achieved.

The present application provides a liposome having a double-layer membrane structure and use thereof. The liposome comprises a double-layer lipid membrane formed from (a) a phospholipid, (b) dipotassium glycyrrhizinate or a cationic surfactant, and (c) a polyoxyethylene sterol ether having a hydrophobic end embedded in the double-layer lipid membrane. The liposome of the present application has excellent stability and can be stably present in a formulation form. Furthermore, a liposome having a double-layer lipid membrane structure with excellent stability can be obtained without using a polyol.

A composition including ionizable lipids is provided. Methods of making the ionizable lipids are also provided. Also provided are compositions forming lipid nanoparticles, wherein the composition includes the ionizable lipid, a helper lipid, a structural lipid or sterol, and a polymer-conjugated lipid. Methods of using the ionizable lipid and lipid nanoparticles are also provided.

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

The present application provides vaccines comprising an inactivated influenza virus and an adjuvant comprising a triterpenoid saponin, a sterol, and an immunostimulatory oligonucleotide comprising CpG. Also provided are methods of using such vaccines.

The invention provides a lipid nanoparticle based on taurochenodeoxycholic acid modification as well as a preparation method and application thereof. The lipid nanoparticle comprises nucleic acid and a lipid carrier encapsulating the nucleic acid, the lipid carrier is prepared from the following components in percentage by mole: 40 to 65 percent of ionized lipid, 5 to 20 percent of structural phospholipid, 20 to 50 percent of sterol component and 0.5 to 5 percent of PEG (Polyethylene Glycol)-lipid, wherein the sterol component comprises cholesterol and taurochenodeoxycholic acid. By replacing cholesterol with taurochenodeoxycholic acid, the membrane order degree and fluidity of the lipid carrier are effectively adjusted, and the targeted delivery and endosome release efficiency of LNP to liver cells is remarkably improved, so that an LNP delivery system can obtain a more remarkable and lasting Lp (a) reduction effect under the same or lower dosage; by optimizing the LNP formula, it is ensured that the LNP preparation has higher controllability and reliability in the production and storage process.

The present disclosure provides a yeast composition enriched in at least one vitamin and / or in at least one sterol that can be used to provide antioxidant activity. The yeast composition can be used to prevent or limit the oxidation of an edible or a drinkable product. The yeast composition can be used to increase the robustness of a fermentative organism during a fermentation. The yeast composition can be used to provide a source of nutrition to a fermentative organism during a fermentation.

The present disclosure provides lipid nanoparticles that are particularly beneficial in inducing an immune response in a subject, particularly by stimulating production of antibodies by the subject. The lipid nanoparticles can include one or more of a low conjugated lipid concentration, a neutral phospholipid, and a concentration of an ionizable lipid and / or sterol that satisfy certain thresholds dependent on the conjugated lipid concentration. The disclosure also provides vaccines, other pharmaceutical compositions, and methods including the provided lipid nanoparticles.