46 results about "Intercellular space" patented technology

Disclosed are multilayered liposomes for transdermal absorption and a method of preparing the liposomes. The multilayered liposomes are prepared using a mixture of oil-phase components comprising squalane, sterols, ceramides, neutral lipids or oils, fatty acids and lecithins, is 200 to 5000 nm in particle size, and is capable of entrapping a physiologically active substance. The multilayered liposomes entrap a larger amount of a physiologically active substance and are structurally stable when encapsulating the physiologically active substance, compared to unilamellar liposomes. Also, they are prepared by a simple and cost-effective process not using a high-pressure homogenizer but using a general homo mixer. Further, since the multilayered liposomes are prepared in a larger size than the intercellular spaces in the stratum corneum, they overcome the tension of surrounding cells when passing through the intercellular spaces and are thus able to penetrate into the dermal layer, compared to nano-sized unilamellar liposomes. Thus, the multilayered liposomes are useful for enhancing the transdermal absorption of physiologically active substances.

A probiotic enzyme ecological-restoration composition uses centella asiatica as a main raw material, uses multiple Chinese herbal medicines including angelica sinensis and the like and food materials as auxiliary materials and is prepared by performing aerobic fermentation and anaerobic fermentation, adding filtrate emu oil, badger oil and horse oil and performing homogeneous processing. The probiotic enzyme ecological-restoration composition can expand the intercellular space of the skin, dredge pores and loosen sweat ducts, promotes the skin to absorb a large amount of beneficial nutritional substances in the composition so as to play the special effects of modification, moisture preserving, moisturizing and immunity enhancement, can rapidly repair the skin, has the obvious effect on scalds, wound infection and scar repair and can also serve as a daily skin care products.

The present invention discloses a method for attaining potato plates which are disease resistant by utilizing agrobacterium-mediated hrap gene. After the combined inoculation of the method and the bacteria allergic protein harpinPss, the allergic reaction caused by harpinPss can be promoted in the intercellular space of all inoculated plates, and the polymeride of harpinPss is dissociated, so the disease resisting effect is better, and the disease resistance of the potato plates can be promoted. The hrap gene is introduced in the potato plates through the agrobacterium-mediated, thus resulting that the potato plates have the ability to resist the late blight and the bacterial wilt. The present invention has the technical proposal that the pre-cultured explants are inoculated in the agrobacterium germ liquid to be soaked, explants after being soaked select the tube seedling which can root after two times of root selections by co-culturing, callus of induce, bud inducting and rootage cultivating and selecting, and the potted transformed plants are transferred after the mass propagation, the transformed plants select positive plants by the methods of the PCR detection and the Southern hybridization detection, and then the potato plates which are disease resistant can be obtained by the in-vivo inoculation resistance identification. The present invention improves the callus of inducing and the bud inducing of the explants, and enhances the agrobacterium-mediated ability. That the roots of the explants are selected by two times can eliminate the false positive plates in the transformation process , avoid the continuing cultivation to the false positive plates, save the labour force and decrease the manufacturing cost.

A method for fermenting carbohydrate-rich crops is provided. Sugar beet, sugar cane, sweet sorghum, tropical maize hybrids and fruits are rich in simple sugars; potato, sweet potato, cassava and yam are rich in starch; and Jerusalem artichoke is rich in inulin. This method uses vacuum infusion to infuse yeast into the intercellular space (apoplast) of the parenchyma tissue. The simple sugars diffuse into the apoplast, come into contact with the yeast and produce ethanol. Ethanol can be extracted from the crop by vacuum stripping or crushing or can be left inside the starchy crop to preserve it. In some variants, pectinase enzymes degrade the parenchyma cell walls to speed up diffusion of simple sugars to the yeast, speed up diffusion of amylase to starch granules or speed up diffusion of inulinase to insoluble inulin.

The invention discloses a method for culturing adult distal end pulmonary artery smooth muscle cells, which comprises following steps: firstly, separating pulmonary artery smooth muscle cells from an adult distal end pulmonary artery smooth muscle layer which is obtained and culturing primary cells, secondly, obtaining the adult distal end pulmonary artery smooth muscle cells which are subcultured, taking out the primary cells and cleaning with PBS solution after the primary cells in the first step grow to the logarithmic growth phase, adding trypsin solution 0.3-0.5ml 0.1%-0.2%, digesting for 2-3 minutes under the temperature of 35-37 DEG C, gettering the trypsin solution when cells appear retraction and the intercellular space is enlarged, adding complete culture medium 3-5ml to enable the trypsin solution to form cell suspension, inoculating in a new culture bottle, changing medium each three days, then, finishing culturing subculture cells, and obtaining the culturing adult distal end pulmonary artery smooth muscle cells which are subcultured. The method for culturing has stable technique and good repeatability, and the cells which are cultured have uniform forms with good growth and have the forms and characteristics of typical smooth muscle cells.

The invention provides an umbilical cord tissue freeze preservation and unfreezing method. According to the umbilical cord tissue freeze preservation and unfreezing method, cell aggregate freeze preservation is adopted instead of single cell freeze preservation, so that cell damage apoptosis rate is reduced, and survival rate is increased; cell adhesion molecules are added to promote bonding of cells, reduce the amount of single cells, and increase cell survival rate; ultrasonic treatment is adopted so as to ensure full penetration of a refrigeration protective solution and an unfreezing protective solution into intercellular spaces, solutions are dispersed into small liquid drops, and the permeability is improved; a cell activity inhibitor is adopted for reversible inhibition of physiological activities in cells, and improving of freeze preservation effect without influencing cell activity. The umbilical cord tissue freeze preservation and unfreezing method is capable of reducing physical and chemical damages on cells in freezing and unfreezing process, and increasing cell survival rate and unfreezing rate, so it is convenient for subsequent using.

A method of detecting and identifying bacteria, micro-organisms or plants in a liquid or gaseous medium, the bacteria, micro-organisms or plants being of the kind which produce signaling molecules in intercellular space. The method includes positioning a biosensor in the liquid or gaseous medium, the biosensor having a biolayer matched to specific signaling molecules to be detected whereby the biolayer is reactive thereto in a manner which varies operation of the sensor. Such variation of the operation of the biosensor is detected to thereby determine the presence and purpose of the bacteria, micro-organisms or plants in the liquid or gaseous medium.

The invention discloses a fermented Chinese herbal medicine for treating livestock gastrointestinal tract parasitic diseases. The fermented Chinese herbal medicine is prepared by fermenting the following Chinese medicine raw materials in parts by weight: 5-15 parts of wild carrot fruit, 5-15 parts of quisqualis indica, 5-15 parts of areca-nut, 5-15 parts of fructus ulmi, 5-15 parts of stone-like omphalia, 10-20 parts of male fern rhizome, 1-10 parts of dried ginger, 1-10 parts of sliced white aconite, 1-10 parts of dark plum, 1-10 parts of myrobalan, 1-10 parts of rheum officinale, 1-10 parts of radix stemonae, 1-10 parts of elecampane and 1-10 parts of torreya seeds. Probiotics of the fermented Chinese herbal medicine can generate various enzymes such as cellulose, ligninase, amylase and protease in the growing process, and because of the enzymes, cell membranes of Chinese medicine materials can be broken, the intercellular space is increased, and effective components and active substances in cells can be extracted and utilized to the maximum extent, and can be efficiently assimilated by animal organism bodies.

The invention provides a composition for treating senile dementia and a preparation method and application of the composition, and belongs to the technical field of biological medicines. The providedcomposition comprises the following components by mass: 0.5-1 part of coenzyme Q10, 0.05-0.2 part of polysorbate, 0.3-0.8 part of medium-chain fatty acid triglyceride, 3-8 parts of gelatin, 3-8 partsof glycerinum and 3-8 parts of sorbitol. When the packing specification of the composition is less than or equal to 50 nm, the composition effectively penetrates through the blood brain barrier, enters the deep brain and reaches a target neuron; the neuron chondriosome is efficiently activated, the activity of chondriosome cytochrome C is improved, the synthesis of ATP (Adenosine Triphosphate) isstrengthened, the energy is provided for the neuron; the formaldehyde in the brain is reduced, the effect of promoting the formation of age pigments of formaldehyde is reduced, the purpose of clearingaway blocking of an intercellular space is fulfilled, the flow of an intercellular fluid is restored; the composition is non-toxic for the human body, has the capability of boosting immunity, and improves the memory of a dementia model mouse.

The invention relates to a pharmaceutical compound for antioxidant protection of cells, tissues and entire organism against hyperproduction of fee radicals. The inventive compound comprises an effective quantity of at least one antioxidant in the form of a main acting component. Said antioxidant is selected from a group consisting peroxiredoxine, a fragment thereof, dihydrolipoic acid and the combinations thereof with acceptable pharmaceutical additives. The inventive method for preventing and curing mammals consists in contacting the effective quantity of the compound with intercellular space of tissue, organ and entire mammal organism. Low toxicity of the compound makes it possible to increase the efficiency of treatment of multifactor actions on an organism, for example radiation, thermal, chemical burn, wounds and hurts as a result of disasters and fires.

The invention relates to an application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines or kits for treating LPS-induced respiratory distress syndrome, the sequence of the high-density protein mimic peptide is Ac-FAEKFKEAVKDYFAKFWD-NH2. The Reverse-D-4F can inhibit pulmonary edema of an LPS-induced rat lung injury model, inhibit in-vivo inflammatory factors, alveolar wall thickening and intercellular space erythrocyte and leucocyte infiltration, as well as the generation of lung vascular endothelial inflammation markers (ICAM-1, VCAN-1 and iNOS) and the endothelial marks (eNOS and vWF) are increased. Reverse-D-4F promotes mobilization of EPCS of the LPS-induced rat lung injury model, no matter whether in vitro or in vivo, the Reverse-D-4F can be used for repairing the injured function of the EPCS of the LPS-induced rat lung injury model.

A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.

The invention discloses a composite cell vagina shrinking lotion for female nursing and belongs to the field of vagina shrinking nursing. The composite cell vagina shrinking lotion contains natural traditional Chinese medicinal material ingredients such as lily bulbs, cnidium monnieri, saffron crocus and fructus evodiae; extracted titanium is subjected to extraction and mixing by adopting Sanxianspearwort; the composite cell vagina shrinking lotion plays roles in humidifying and remedying and recuperating elasticity of female vaginal muscle and mucosa, cells can be regenerated, and slack vaginae can become compact and lubricated; auxiliaries such as hyaluronic acid and rose essential oil are also added, so that moisture can enter human body intercellular spaces to play roles in preservingmoisture and recuperating elasticity of the vaginal muscle and mucosa, and meanwhile, odors can be improved; in addition, growth conditions of the vaginal mucosa can be improved, so that the composite cell vagina shrinking lotion has the actions of resisting inflammations, inhibiting generation of pathogenic bacteria and keeping skin bright and clean.

The invention discloses an extraction method of deoxydihydroxanthoangelol H chalcone suitable to serve as a liver cancer targeted drug. The preparation process consists of: alcohol-water extraction of Angelica keiskei, removal of chlorophyll with petroleum ether, silicagel column adsorption of deoxydihydroxanthoangelol H chalcone, water elution of saccharides and other water-soluble impurities, elution of deoxydihydroxanthoangelol H chalcone with a mixed solvent of n-hexane and ethyl acetate, and secondary recrystallization with methanol, thus obtaining a deoxydihydroxanthoangelol H chalcone refined product. The method provided by the invention has a simple and easily controllable process, and the obtained deoxydihydroxanthoangelol H chalcone product has high purity. Living animal contrast experiments show that: the Angelica keiskei deoxydihydroxanthoangelol H chalcone prepared by the method provided by the invention has a significant inhibiting effect on liver cancer intercellular space blood vessel growth, promotes cancer cell necrosis, is accord with the targeted drug characteristics, can be used as a liver cancer tumor angiogenesis inhibitor, and is suitable to serve as a liver cancer targeted drug.

The present invention discloses a plasma mask. The plasma mask comprises a mask, a connecting line and a power source control system; and the mask is connected with the power source control system through the connecting line. At present, it has not been found that a dielectric barrier discharge (DBD) plasma technology is applied to the mask cosmetology; and existing mask technologies cannot solve the problem of skin residual particles, do not have sterilization and disinfection functions and cannot prevent the problem of bacterial infection and inflammation. The plasma mask provided by the present invention is used for cleaning the skin; and a high energy electric field generated by plasmas can penetrate the surface layer of the skin, penetrate pores and intercellular spaces, bombard grease blocking the gaps of the pores, adsorb tiny particles, crumbs and cosmetic residues hidden in skin folds or the pores, disinfect and sterilize the skin, can clean the skin and can prevent the inflammation of the skin caused by bacterial infection. If a user frequently uses the plasma mask, the skin can be kept healthy. The plasma mask is convenient to carry and is suitable for daily domestic use.

The invention discloses a skin cuticle repair essence microcapsule and a preparation method thereof. The microcapsule is a transparent spherical soft capsule of a core-shell structure, wherein the capsule wall is a sericin shell membrane, the capsule core is a skin cuticle repair essence, the mass ratio of the capsule wall to the capsule core o is (1-3):1, and the diameter of the microcapsule is 1-5 mm; the skin cuticle repair essence comprises, by weight, 11-28 parts of ceramide, 8-19 parts of cholesterol, 5-18 parts of lecithin, 3-7 parts of vitamin A, 1-8 parts of vitamin E, 1-3 parts of histidine, 1-3 parts of lysine, 1-3 parts of arginine, 3-9 parts of hyaluronic acid, 3-11 parts of sweet almond oil and 2-9 parts of jojoba oil. All components of the skin cuticle repair essence microcapsules act synergistically to promote the absorption rate of ceramide, cholesterol and other raw materials; the microcapsule can be well integrated into intercellular space and accelerate recovery ofthe barrier function of the damaged skin cuticle, thereby improving the skin condition.

The invention provides a traditional Chinese medicine liniment for treating scald, which is prepared through repeated verification by clinical tests and meets the theory of traditional Chinese medicines. The traditional Chinese medicine liniment is prepared from the following traditional Chinese medicine raw materials: cortex lycii radicis, scutellaria baicalensis, golden cypress, coptis chinensis, cortex dictamni, arisaema consanguineum schott, rhizoma atractylodis, lithospermum, fructus cnidii, rheum officinale, frankincense, myrrh, moutan barks, badger fat, camphor and agastache rugosus. The traditional Chinese medicine liniment provided by the invention has the functions of moisturizing, moistening skins, stretching and relieving pains, promoting blood circulation to remove blood stasis, clearing away heat and toxic materials and eliminating necrotic tissues and promoting granulation, and can permeate into necrotic tissues and intercellular spaces to realize unobstructed drainage, so that solid necrotic tissues are softened and loosened and finally are converted into liquid substances to be discharged. Meanwhile, the liniment maintains the microenvironment of cell survival and promotes regeneration and repair of skin tissues and organs.

A method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop is characterized that the method is a quantitative method provided for evaluation of structural indexes of the baked tobacco leaves and including: acquiring a 450-times multiplied image of a surface cell structure of initially-baked tobacco leaves by the aid of a scanning electron microscope, reading total pixel value of the image by the Photoshop software, selecting an intercellular space portion accurately by means of a magic wand, a masking, a brush, an eraser and the like, and computing surface intercellular space rate of the tobacco leaves by the ratio of pixel value of the intercellular space portion to the total pixel value of the image. The method for measuring surface intercellular spaces of the baked tobacco leaves has the advantages that by acquisition of the scanning electron microscope and processing of the Photoshop software, quantitative data of the intercellular spaces of the tobacco leaves are acquired so as to evaluate the structure of the tobacco leaves objectively. According to continuous multi-times measuring results of tobacco leaves from different production places, the method is high in accuracy, the variation coefficient of the measured results of the same sample is kept within 2.5%, and fit goodness of the evaluation results and the judgment results of specialists is high (R2=0.98).

The invention discloses a method for improving the stability of fruit and vegetable storage. In a drying process of fruits and vegetables, fruit and vegetable glass-transition temperature (Tg) is increased by reducing the relaxation behavior of fruit and vegetable semi-bound water, so that the stability of fruit and vegetable storage is improved. After the method is adopted, a glass-transition law of hot air-dried carrots in different water-bearing states is obtained by using a dynamic stability control (DSC) technology. When the water contents of the carrots are higher, free water in vacuoles dominates, the Tg is substantially the same although the water contents of samples are different, and the value of the Tg is 48.4+/-1.04 DEG C. When the water contents of the carrots are less than or equal to 0.41g/g dry matter, T22 of the semi-bound water is reduced by 12.4ms and 11.8ms along with the semi-bound water cytoplasm and intercellular spaces of the samples at the temperature of 50 DEG C and 60 DEG C, and the Tg is respectively increased by 6.79 DEG C and 22.7 DEG C. The results prove that in the hot air-dried carrots, the reduction of the relaxation behavior of the semi-bound water can be taken as an effective means for increasing the Tg. The method has certain guiding significance for vegetable storage, processing condition improvement and quality control.

The invention is applied in the therapy of brain and spinal cord tumors, degenerative, hypoxic, ischemic diseases and traumatic injuries of the central nervous system (CNS) and other diseases of humans and animals. Object of invention is to provide target delivery of signaling substance to the pathological area of an organism, triggering in due time and long-time support of active signaling intercellular action of specific therapeutic character of a healthy and well controlled stem cell (SC) onto controlling system of defective cells. The preparation of SC with reprogrammed cell signaling comprises the basic preparation of SC, the membrane, and/or nucleus, and/or cytoplasm of which contains implanted protein or pharmaceutical able to regulate signaling pathways of SC and cells of pathological focus in a mammal organism, provisionally encapsulated in nanocontainers of less than 100 nm size, obtained from biodegradable material, intact for organelles and compartments of SC of the basic preparation. The material has a set biodegradation period in a mammal organism to provide programmed exit of protein or pharmaceutical in intra- or intercellular space thus reprogramming signal transduction of key genes in the desired therapeutic orientation of physiologic events in the cell cycle directly in the pathological area or tissue of an organism.