13 results about "Intercellular space" patented technology

The invention relates to an oral cavity moisturizing spray which is characterized by comprising the following components in percentage by mass: 3-5% of an inducer, 1-5% of a preservative and the balance of water. 0.1 to 0.3 percent of accelerant; 0.2%-0.5% of a humectant; 2-3% of mucopolysaccharide; and the balance of water. Through scientific compatibility of the four functional components of the inducer, the accelerant, the humectant and the mucopolysaccharide compound, a synergistic effect chain of induced permeation, absorption promotion, long-acting water locking and film-forming protection is formed; in addition, glycerol is used as a permeation inducer, and oral mucosa intercellular spaces are quickly opened by utilizing the small molecule characteristic (molecular weight is 92Da) of glycerol, so that subsequent active ingredients are easier to permeate. Compared with the traditional polyethylene glycol inducer, the glycerol has higher biocompatibility and mucous membrane affinity.

The invention relates to the technical field of cosmetics, and particularly discloses a recombinant three-type collagen cosmetic for sensitive skin care and a preparation method of the recombinant three-type collagen cosmetic. The invention relates to the technical field of cosmetics, in particular to a facial mask which is prepared from, by weight, 12-18 parts of recombinant three-type collagen, 4-7 parts of ceramide 3, 6-9 parts of squalane, 8-10 parts of centella asiatica extract, 3-5 parts of sodium hyaluronate, 1.5-2.5 parts of allantoin and 5-8 parts of beta-glucan, the recombinant three-type collagen serves as a core component, and key support is provided for reconstruction of skin barriers by quickly filling intercellular spaces and strengthening the basic structure of skin. Meanwhile, other compounds are matched to lock skin moisture, reinforce an intercellular lipid bilayer and make a skin barrier more compact, meanwhile, the centella asiatica extract is added, the skin stress response can be rapidly pacified, release of inflammatory mediators such as histamine in the skin is inhibited, uncomfortable symptoms such as skin redness, itching and stabbing pain are relieved, and the skin care product is suitable for being used for skin care. The skin is in a long-term sedative state.

The invention belongs to the technical field of pharmaceutical preparations, and particularly discloses a colchicine external nano latex agent as well as a preparation method and application thereof.The colchicine external nano latex agent is prepared from the following raw materials in percentage by mass: 0.1%-1.2% of colchicine, 0.5%-25% of an oil phase matrix, 2%-20% of a surfactant, 2%-15% of a cosurfactant, 0%-15% of a transdermal absorption enhancer, 3%-3.5% of a gel matrix, 0%-0.5% of a pH regulator and the balance of water, totaling 100%. According to the colchicine external nano latex agent, the optimal prescription is obtained by applying a pseudo-ternary phase diagram and combining with D-optimal mix design, the particle size of the nano emulsion is stably controlled to be about 14 nm, and the small particle size means faster Brownian movement and stronger penetration of skin cuticle gaps or improvement of the infiltration capacity through appendages such as hair follicles, so that the colchicine external nano latex agent has a good application prospect. The skin penetration efficiency is higher than that of other prescriptions with larger particle sizes, and the nanoemulsion with small particle size hardly shows lag time.

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to an external traditional Chinese medicine composition for treating sport injury and chronic pain and a preparation method of the external traditional Chinese medicine composition. The preparation method comprises the following steps: extracting other components including monkshood, cassia twig, clove, liquorice, safflower, caulis sinomenii and peach kernel with ethanol for multiple times to obtain a traditional Chinese medicine extracting solution, and compounding the traditional Chinese medicine extracting solution with the agilawood extract to obtain the plant small-molecule traditional Chinese medicine composition. The traditional Chinese medicine composition directly permeates through skin and intercellular space to reach a focus and a target spot, accelerates basic metabolism through lymphatic circulation, can quickly remove inflammation, relieve swelling and pain, release adhesion, repair damaged tissues and accelerate regeneration of damaged tissue cells, has a remarkable curative effect on sport injury, chronic pain and the like, and is free of toxic and side effects. And the raw materials are pure plants, are safe and free of side effects, are easy for large-scale production, and have very good market application prospects.

This invention discloses a uniform, ultrafine polymer nanomicelle, its preparation method, and its applications. The polymer nanomicelle is first formed by modifying a material based on poly(lactic-co-liquid) with ligands containing double bonds to create a polymer containing unsaturated bonds. The polymer then encapsulates berberine (BBR) via a self-emulsification process, followed by UV cross-linking and pH adjustment. The polymer nanomicelle uses materials obtained from FDA-approved excipient PLGA through simple modification, and subsequently, drug-loaded nanomicelles with a particle size of less than 10 nm are prepared via self-emulsification. Due to their small particle size, the nanomicelles can be transported through the intercellular spaces of epithelial cells, effectively improving drug bioavailability. Combined use with the intestinal permeabilizer SNAC can further enhance the transintestinal absorption capacity of the nanoparticles. The polymer nanomicelles exhibit high uniformity, good stability, good safety and biocompatibility, and no toxic side effects, making them a promising candidate for treating type 2 diabetes.

This invention discloses a method for removing hair from animal skin suitable for food processing, belonging to the field of food processing technology. The method employs a two-stage process of "pretreatment + targeted hair removal." First, glycosidase pretreatment degrades the protein-polysaccharide complex in the intercellular spaces of the epidermal cells, clearing the permeation barrier without damaging subcutaneous fat. Then, charge adaptation technology is used, utilizing enzymatic hydrolysis to naturally lower the pH of the system. Initially, the enzyme molecules carry the same charge as the hair follicle permeation channels, enhancing permeation efficiency. Later, as the pH decreases, the charge of the enzyme molecules reverses, targeting and binding with the charge of the matrix surrounding the hair follicle, achieving hair removal. This method overcomes the limitation of two-sided permeation, achieving highly efficient one-sided permeation hair removal, avoiding the need for degreasing and preserving subcutaneous fat to the maximum extent. Furthermore, the process completely avoids the use of chemical additives that pose food safety risks or damage flavor. It offers high hair removal efficiency and minimal loss of skin collagen, providing a safe and efficient hair removal solution for animal skin food processing.

The invention discloses an in-vitro kidney perfusion preserving fluid and a preparation method thereof, and belongs to the technical field of organ preservation. The preserving fluid consists of the following components: polyethylene glycol, adenosine, sodium sulfate, magnesium sulfate, tetramethylpyrazine, potassium citrate, disodium hydrogen phosphate, sodium dihydrogen phosphate, mannitol and sterile water. The method can relieve cell edema caused by low-temperature storage, prevent cell acidification, prevent cell gap swelling and prevent oxygen free radical damage in the reperfusion process, and provides a regenerated high-energy phosphate compound substrate: the high-energy phosphate compound substrate, such as adenosine, is added to keep the intracellular environment stable, so that the regeneration of the high-energy phosphate compound substrate is realized. The purposes of complete localization, cost saving and better kidney perfusion effect are achieved.

The application discloses a transdermal drug delivery device, comprising: a drug container for storing a drug solution; the metal sheet is provided with a micro hole, and the micro hole is communicated with the medicine container; the piezoelectric substrate is of an annular hollow structure, the metal sheet is arranged on the piezoelectric substrate, and the metal sheet and the piezoelectric substrate form a transducer; wherein the piezoelectric substrate vibrates to generate capillary waves on the metal sheet, so that a medicine solution in the medicine container is jetted into liquid drops through the micropores, and when the liquid drops are jetted onto the skin cuticle, gas can be wrapped to form bubble cloud clusters; the bubble clouds have longitudinal pushing force for accelerating the medicine to move towards the deep layer of the skin in the vertical direction of the skin, and shock waves generated by the bubble clouds have transverse pushing force for enlarging intercellular spaces in the horizontal direction of the skin, so that the medicine penetrates through the cuticle of the skin.

The present invention discloses a liquid biopsy marker and a therapeutic target for clear cell renal cell carcinoma and their applications, belonging to the field of clinical diagnosis and treatment. Through bioinformatics analysis, it is found that the mRNA of PD-L2 in clear cell renal cell carcinoma is highly expressed after carcinogenesis, and is higher than the expression level of the mRNA of PD-L1, and is also related to poor staging. Further immunohistochemistry is used to verify the expression and localization of PD-L2 in clear cell renal cell carcinoma and adjacent tissues, and it is found that PD-L2 is highly expressed in the intercellular space after carcinogenesis of clear cell renal cell carcinoma, indicating an increase in its secretion after carcinogenesis. This application reveals the role and mechanism of exosomal PD-L2 in the development of clear cell renal cell carcinoma with or without immunity, indicates its potential as a liquid biopsy detection index for predicting the prognosis of clear cell renal cell carcinoma, and provides a theoretical basis for exosomal PD-L2 to become a therapeutic target for clear cell renal cell carcinoma.

The invention provides a drought-enduring plant selection system based on osmotic regulation substance dynamic monitoring, and relates to the technical field of plant phenotype and molecular characterization detection, and the drought-enduring plant selection system comprises S1, a self-cleaning microfluidic sampling module which comprises a microneedle array and a programmable microvalve, the self-cleaning microfluidic sampling module can periodically and quantitatively extract trace samples from intercellular fluid or phloem fluid on the surface of a plant living tissue to be detected in a non-destructive mode, channel blockage is prevented through self-cleaning circulation, and the molecular imprinting sensitization microcavity spectrum sensing module is in fluid communication with the self-cleaning microfluidic sampling module. According to the drought-enduring plant selection system based on the dynamic monitoring of the osmotic regulating substance, a self-cleaning microfluidic sampling and molecular imprinting sensitization microcavity spectrum sensing module is integrated on the surface of a living tissue; the system can realize non-destructive sub-micron level continuous on-line quantitative monitoring on osmotic regulation substances such as proline, soluble sugar and betaine in a plant living body state, and outputs a concentration change curve in real time.

The invention relates to the technical field of oral liquid preparation, in particular to a plant exosome oral liquid preparation method and system based on powder-liquid separation. Performing the following operations on each oral liquid plant raw material in the oral liquid plant raw material set: performing plant exosome extraction operation on the oral liquid plant raw materials to obtain an initial solid phase extracting solution and an initial stable intercellular fluid, and respectively integrating the initial solid phase extracting solution and the initial stable intercellular fluid, the preparation method comprises the following steps: preparing a solid-phase extracting solution and a stable intercellular fluid, carrying out multi-stage centrifugal separation operation according to the solid-phase extracting solution and the stable intercellular fluid to obtain an upper-phase exosome concentrated solution, carrying out exosome nano reconstruction on the upper-phase exosome concentrated solution to obtain a drug-loaded exosome suspension, and preparing the plant exosome oral liquid. According to the method, the structural integrity and biological activity of the exosome can be protected while efficient solid-liquid separation is realized.

The invention provides a method for promoting skin penetration of a medicament, and relates to the technical field of cosmetics, and the method comprises the following steps: S1, a primary stirring step: adding 50-70 parts by weight of base oil, 5-10 parts by weight of an emulsifier, 2-5 parts by weight of a thickening agent and 80-90 parts by weight of a skin care agent into a stirrer; according to the invention, the permeation efficiency and effect of the medicinal components are obviously enhanced through multiple permeation promotion means. A slight vacuum environment is formed on the skin surface through a local negative pressure permeation method, the physical barrier of the cuticle is reduced, the resistance of the skin to medicine permeation is reduced, and deep absorption of components is promoted. When the oscillating electric field penetration method is applied to the cuticle, tiny vibration is generated to change the intercellular space of the skin, so that the permeability of the medicinal components is enhanced. According to the heat energy local conduction permeation method, a warm environment is formed after the ointment is in contact with the skin through particles which slowly release heat, and the release of drug activity is improved while drug diffusion is accelerated.

The invention relates to a whitening composition, modified glutathione and a preparation method thereof. The preparation method of the modified glutathione specifically comprises the following steps: 1) obtaining a liposome suspension, performing ultrasonic dispersion, mixing the liposome suspension with hyaluronidase and a cross-linking agent, and stirring to obtain a HAase-Lip suspension; and 2) mixing glutathione with the HAase-Lip suspension, carrying out a stirring reaction, and carrying out ultrasonic treatment to obtain the glutathione-Lip composite material. The phospholipid bilayer vesicle structure of Lip can form a physical barrier, so that the efficacy attenuation is greatly delayed. Meanwhile, a skin-like lipid structure of Lip can be fused with a cuticle lipid bilayer, the nanoscale particle size of Lip can be matched with the cuticle gap size, and Lip penetrates through the cuticle by means of the permeation mechanism of Lip; on the other hand, HAase has the capability of degrading hyaluronic acid on the surface layer of the skin, assists Lip and internal GSH to permeate into the deep layer of the epidermis, and reduces the damage of transdermal penetration to the skin barrier.