145 results about "Hyaluronidase" patented technology

Described herein are antibodies, or antigen binding fragments thereof, that bind OX40L and IL- 13 in combination and methods of use thereof. In certain aspects, described herein are methods of inhibiting OX40L and IL- 13 biological activity. In certain aspects, described herein are pharmaceutical compositions comprising the anti-OX40L and anti-IL-13 antibodies, or antigen binding fragments thereof. In certain aspects, the antibodies and methods described herein are used for treatment of an inflammatory disease or disorder (e.g., associated with elevated levels of OX40L and / or IL- 13). Also described herein are compositions and combinations comprising an antibody or an antigen binding region that binds OX40L, an antibody or antigen binding region that binds IL- 13, and a hyaluronidase or variant thereof, as well as related methods of treating an inflammatory disorder or disease in a human patient by administering such compositions and combinations.

The invention belongs to the technical field of medical biological materials, and particularly relates to a sodium hyaluronate gel regeneration filler for injection and a preparation method and application thereof.The sodium hyaluronate gel serves as a carrier to disperse acellular matrix particles, the sodium hyaluronate gel forms a micron-sized porous structure in the cross-linking process through a microbubbling technology, and the sodium hyaluronate gel is prepared into the sodium hyaluronate gel regeneration filler for injection and the preparation method and application of the sodium hyaluronate gel regeneration filler. A local cross-linked network is strengthened by combining a secondary cross-linking process, high elastic modulus is realized while the content of sodium hyaluronate is reduced, so that the porous structure has excellent instant filling and shaping capability and controllable degradation behavior, the porous structure can promote permeation of hyaluronidase, the degradation period of the hyaluronidase is matched with the tissue regeneration process, and the tissue regeneration effect is improved. And the acellular matrix particles can continuously induce adhesion, migration, proliferation and differentiation of endogenous cells of tissues, synergistically promote in-situ tissue regeneration, effectively reduce repeated injection requirements and reduce long-term complication risks, and have a good application prospect in soft tissue filling and repairing.

The invention relates to an enzyme response antibacterial carbon dot and a preparation method and application thereof.The carbon dot takes a copper-doped carbon dot as a core and is covalently grafted with a hyaluronic acid shell layer through amidation reaction to form a composite nano material Cu-CDs (at) HA, specifically, soluble copper salt and folic acid are subjected to a hydrothermal reaction to obtain the copper-doped carbon dot, carboxyl is activated through EDC / NHS in a buffer system, and the carbon dot is prepared. And performing grafting reaction with hyaluronic acid to obtain a target product. The hyaluronidase-containing antibacterial composition can be specifically degraded and release antibacterial components under the action of hyaluronidase, shows good bacterial responsive bactericidal performance, has the minimum inhibitory concentrations of 8 g / mL and 16 g / mL for staphylococcus aureus and escherichia coli respectively, and has peroxidase-like activity and free radical scavenging capacity. The antibacterial carbon dots can be used for preparing intelligent wound dressings, antibacterial coatings and other biomedical materials, and have the advantages of responsive drug release, efficient antibacterial property and promotion of wound repair.

Described herein are antibodies, or antigen binding fragments thereof, that bind OX40L and IL-4Rα in combination and methods of use thereof. In certain aspects, described herein are methods of inhibiting OX40L and IL-4Rα biological activity. In certain aspects, described herein are pharmaceutical compositions comprising the anti-OX40L and anti-IL-4Rα antibodies, or antigen binding fragments thereof. In certain aspects, the antibodies and methods described herein are used for treatment of an inflammatory disease or disorder (e.g., associated with elevated levels of OX40L and / or IL-4). Also described herein are compositions and combinations comprising an antibody or antigen binding region that binds OX40L, an antibody or antigen binding region that binds IL-4Rα, and a hyaluronidase or variant thereof, as well as related methods of treating an inflammatory disorder or disease in a human patient by administering such compositions and combinations.

The invention discloses a tissue dissociation liquid, a tissue dissociation method and application thereof, and relates to the technical field of cell biology. In a DMEM / F12 culture medium, the tissue dissociation liquid only contains the following raw materials in concentration: 0.5 to 3 mg / mL of type II collagenase, 0.5 to 2 mg / mL of type IV collagenase, 0.5 to 2 mg / mL of hyaluronidase, 10 to 18 [mu] M of Y27632 and 80 to 150 [mu] g / mL of primocin. The tissue dissociation liquid provided by the invention can dissociate normal tissues or tumor tissues into single cells or cell clusters, the single cells obtained by the digestion method are high in proliferation rate and easy to successfully culture, and the tissue dissociation liquid has the technical advantages that the cell yield and survival rate are maintained at a relatively high level. Therefore, the tissue dissociation liquid provided by the invention has good application prospects in tumor tissue dissociation, cell line construction and organoid model construction.

The present invention provides a composition for reducing or eliminating vascular occlusion caused by hyaluronic acid soft tissue dermal fillers. [Solution] This disclosure provides for compositions comprising hyaluronidase, apparatus for housing such compositions, and methods and uses of such compositions and apparatus for reducing or eliminating hyaluronic acid-induced blockages in one or more blood vessels supplying an individual's eye; methods and uses of such compositions and apparatus for reducing or inhibiting vascular occlusion of an individual's eye; and methods and uses of such compositions and apparatus for reducing or inhibiting hyaluronic acid-induced vision loss in an individual.

Provided herein is a hypervesiculating Escherichia coli Nissle (ΔECHy) bacterium engineered to produce outer membrane vesicles (OMVs), said OMVs packaging a fusion peptide including cytolysin A (ClyA) and hyaluronidase (Hy). Also provided are methods of remodeling a tumor, methods of treating cancer, and pharmaceutical compositions including the engineered ΔECHy bacterium.

The present invention relates to a microneedle structure and a composition thereof that can effectively deliver a drug locally or systemically within the body by penetrating the dermis layer through the transdermis without causing damage to the skin by including a biodegradable composition containing a drug and hyaluronidase.

This invention provides an extract of *Elaeocarpus yunnanensis* from Yunnan and its preparation method and application. The preparation method includes the following steps: (1) crushing *Elaeocarpus yunnanensis*, mixing it with an enzyme, and then using a solvent for negative pressure cavitation extraction to obtain a crude extract of *Elaeocarpus yunnanensis*; (2) adsorbing and eluting the crude extract of *Elaeocarpus yunnanensis* obtained in step (1) using a macroporous resin to obtain the *Elaeocarpus yunnanensis* extract. The method provided by this invention can effectively extract active substances from *Elaeocarpus yunnanensis*. The obtained *Elaeocarpus yunnanensis* extract has high geranium content, high total flavonoid content, good antioxidant effect, high hyaluronidase inhibitory activity, good anti-inflammatory effect, and high collagenase activity inhibition rate. The preparation method is economical and environmentally friendly, and the reagents used can be recycled and reused. It can be used as an active substance in health care products and cosmetics.

A pharmaceutical combination for subcutaneous administration and use thereof are provided. The pharmaceutical combination as described herein for subcutaneous administration includes a hyaluronidase and an antibody-drug conjugate. The antibody-drug conjugate includes a fragment of a bioactive molecule, and the bioactive molecule has moderate toxicity. The pharmaceutical combination can achieve a larger volume and a larger dose of administration, and the side reaction is not enhanced.

The invention relates to compositions and methods for treating cancer in a patient comprising subcutaneously administering a PD-1 antagonist, e.g., an anti-PD-1 antibody (e.g. pembrolizumab), or antigen binding fragment thereof, with or without hyaluronidase every six weeks, in specific amounts to the patient. In specific embodiments, the amount of anti-PD-1 antibody, or antigen binding fragment thereof, is about 600 mg to about 1000 mg. In specific embodiments, the administration occurs about every three weeks, and the amount of anti-PD-1 antibody, or antigen binding fragment thereof, is about 300 mg to about 500 mg. In certain embodiments, the PD-1 antagonist is pembrolizumab, or an antigen binding fragment thereof. Also provided are compositions and kits formulated for subcutaneous administration comprising a particular dosage of an anti-PD-1 antibody, or antigen-binding fragment thereof, and uses thereof for treating cancer.

The present invention relates to a freeze-dried injectable formulation comprising beta-nicotinamide mononucleotide, an injectable composition comprising same, and a preparation method therefor. In addition, the present invention relates to a pain relief injectable composition and a preparation method therefor, the composition comprising beta-nicotinamide mononucleotide and hyaluronidase, and having a pH of greater than 4 and 8 or less.

The application discloses a chimeric antigen receptor targeting CEA and a T cell containing the chimeric antigen receptor targeting CEA. The chimeric antigen receptor is composed of a nanobody recognizing a CEA antigen, an extracellular hinge region, a transmembrane region, an intracellular signal region, a self-cleavage polypeptide T2A and a hyaluronidase in sequence. The chimeric antigen receptor can efficiently recognize the CEA antigen, and CD28 and CD137 are used as a costimulatory signal region to activate T cells, thereby playing a cellular immune role, and specifically killing CEA-positive tumor cells, and thus having an important application prospect in the field of tumor cell immunotherapy.

The invention belongs to the technical field of cosmetics, and particularly relates to a soothing and repairing composition and application thereof. The soothing and repairing composition provided by the invention is prepared from the stephania tetrandra extract, the phoenix dactylifera fruit extract, the flower extract and the mirabilis jalapa extract through specific compatibility, and all the components generate a synergistic effect, so that the transdermal absorption of active ingredients can be remarkably enhanced; experimental data show that the composition can effectively improve the hyaluronidase inhibition rate and the TNF-alpha inhibition rate, and has excellent soothing and repairing effects; the preparation method of the composition is simple and convenient to operate and easy to realize industrial large-scale production, and has a good industrial application prospect; when the composition is applied to the essence, the TEWL value improvement rate and the a * value improvement rate of the essence can be remarkably improved, and the soothing and repairing effects are obvious; and human body patch test results of the essence show that the essence is mild and non-irritating, so that the composition disclosed by the invention has wide market application value in skin care products.

Described herein are compositions and combinations comprising an antibody or antigen binding fragment thereof that binds thymic stromal lymphopoietin (TSLP), an antibody or antigen binding fragment thereof that binds Interleukin-4 Receptor Alpha (IL-4Ra), and a hyaluronidase or variant thereof, as well as related methods of treating an inflammatory disorder or disease in a human patient by administering such compositions and combinations.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The invention discloses a fluorescent reagent composition for detecting hyaluronidase and application thereof, and is characterized in that the fluorescent reagent composition comprises a sodium hyaluronate or hyaluronic acid solution, a magnetic nano material Fe3O4NPs solution and a fluorescent nano material UCNPs solution, the detection of hyaluronidase comprises the following steps: adding a to-be-detected urine sample containing hyaluronidase into a sodium hyaluronate solution to degrade sodium hyaluronate; adding a fluorescent nano-material UCNPs solution into the reaction product, enabling the fluorescent nano-material UCNPs solution to generate electrostatic adsorption with the unhydrolyzed sodium hyaluronate, and outputting a fluorescent signal F0; adding a magnetic nano material Fe3O4NPs solution for magnetic separation, and taking supernate to measure a fluorescence signal F; calculating the concentration of hyaluronidase in the urine sample to be detected by taking F / F0 as an output signal; the kit has the advantages of high sensitivity, strong specificity, high accuracy and simplicity in operation, and is used for ultra-sensitive detection of the bladder cancer biomarker HAase.

The present application relates to the field of sodium hyaluronate preparation, in particular to a preparation method of low molecular weight sodium hyaluronate. Specifically comprising the following steps: stirring and dissolving macromolecular weight sodium hyaluronate powder into water at 45-60 DEG C to obtain a macromolecular weight sodium hyaluronate solution; cooling to 35-45 DEG C, adding a buffer solution and mixing uniformly, then adding papain and hyaluronidase with a mass ratio of (2-3):(3-6) for enzymatic reaction to obtain an enzymatic solution; adding sodium hydroxide solution to the enzymatic solution, adjusting pH to 7.5-9.0, heating to 80-90 DEG C for 20-40 minutes, then filtering to obtain a low molecular weight sodium hyaluronate solution; spray drying, and the process is simple, the conditions are mild, the product structure is not damaged, and the prepared low molecular weight sodium hyaluronate has high purity, uniform molecular weight distribution and good permeability.

The invention relates to the crossing field of biomedical and food technologies, and provides a method for preparing low-molecular-weight hyaluronic acid through high hydrostatic pressure assisted enzymolysis, which comprises the following steps: dissolving sodium hyaluronate by adopting an acetic acid / sodium acetate buffer system, and adding bovine testis-derived hyaluronidase under the condition that the pH value is 4.5-5.5; high hydrostatic pressure enzymolysis at the temperature of 30-45 DEG C, the static pressure of 50-200 MPa and pulse type pressurization is combined, two to three stages of tangential flow ultrafiltration external circulation separation are cooperatively operated, molecular weight distribution is dynamically regulated and controlled through a segmented interception and backflow strategy between first-stage and second-stage membranes, excessive shearing and chromaticity rising are inhibited while the molecular weight interval target and flux are met, and the separation efficiency is improved. At the end of 50-100 kDa, the area proportion is 55-95% or the distribution is continuously changed for two times as long as lt; and after 5%, deactivating enzyme, desalting and freeze-drying to obtain the product. The polydispersity index of the obtained product is 1.05-1.40, micro impurities and residues are strictly controlled, and the product is suitable for cosmetics, functional food and biomedical materials.

Provided are combination dosing regimens comprising administering an antibody-drug conjugate and hyaluronidase. Combinations and compositions containing the antibody-drug conjugate and hyaluronidase are provided. The dosing regimens and combinations are for treating or preventing cancer.

The invention belongs to the technical field of biology, and particularly relates to a nucleus pulposus cell separation and culture method. According to the method, a step-by-step enzyme digestion method is adopted, NB6 collagenase is firstly used for primary digestion, and then compound enzyme liquid composed of trypsin and hyaluronidase is adopted for subsequent digestion; and culturing in combination with a culture vessel coated with a specific concentration of extracellular matrix component and a special culture medium. According to the method, on the premise of not depending on an immortalization technology, the primary nucleus pulposus cells with good activity can be efficiently obtained, stable amplification and long-term passage of the primary nucleus pulposus cells in vitro are realized, and specific marker expression and biological functions of the nucleus pulposus cells can be effectively maintained. The invention provides a stable cell source and technical platform for the development of cell therapy products and the research of spinal degenerative diseases.

The application discloses a composition with soothing and itching-relieving effects and a preparation method and application thereof, and belongs to the cosmetic field; the composition comprises the following components: a composite plant extract, a polyhydric alcohol and water; the composite plant extract is compounded by a polygonatum sibiricum redoute fermentation liquor, a paeonia extract and a long-stemmed grape caulerpa lentillifera extract; and the polygonatum sibiricum redoute fermentation liquor is obtained by composite fermentation of lactobacillus pentosus and bacillus subtilis. The soothing and itching-relieving composition provided by the application has excellent soothing effects, and the soothing and itching-relieving composition provided by the application has excellent in-vitro hyaluronidase inhibition effects, and the in-vitro hyaluronidase inhibition rate reaches more than 60%.

The present invention relates to an injection composition for lipolysis, which is safe and effective, and more specifically, to an injection composition for lipolysis, which comprises: one or more compounds selected from the group consisting of hyaluronidase and pentoxifylline, and one or more compounds selected from the group consisting of hyaluronidase, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline, pentoxifylline and pentoxifylline. 5 to 10% (v / v) of carnitine (Carnitine); and lidocaine (Lidocaine); and normal saline. As a composition having both safety and efficacy, the injection composition for lipolysis of the present invention has an excellent lipolysis effect, does not cause a yo-yo phenomenon over time, and thus has a characteristic of not causing side effects such as skin depression.

The present invention relates to a composition comprising an anti-aging peptide and, more specifically, to an anti-aging cosmetic, food, and pharmaceutical composition comprising the anti-aging peptide. The anti-aging peptide according to the present invention not only exhibits excellent skin permeability, but also demonstrates remarkable antioxidant activity, wrinkle reduction, skin elasticity enhancement, collagenase inhibition, or hyaluronidase inhibition effects, as confirmed experimentally. These findings indicate that the anti-aging peptide of the present invention possesses excellent anti-aging activity and thus can be variously used as a functional material in pharmaceutical, medicinal, cosmetic, and food fields.

The application discloses a multi-component synovitis treatment composition based on repairing cartilage tissue and application thereof, and relates to the technical field of biological medicine, and the composition is composed of glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone and graded molecular weight sodium hyaluronate; the application precisely compounding the graded molecular weight sodium hyaluronate with the glucosamine hydrochloride, the chondroitin sulfate and the dimethyl sulfone according to weight parts, constructs a gradient slow-release and target delivery system of synovitis lesions, specifically inhibits the activity of hyaluronidase in the inflammatory joint cavity by using the dimethyl sulfone, greatly reduces the degradation of the sodium hyaluronate, prolongs the residence time of the active ingredients at the lesion site, realizes immediate analgesia and relieves joint stiffness by quickly forming a lubricating protective film by the high molecular weight sodium hyaluronate, the medium and low molecular weight sodium hyaluronate penetrates the synovial membrane barrier and carries the cartilage repair components to enrich in the cartilage defect area, and the synergistic effect of the components is realized.

A cosmetic composition is provided, which comprises a carrier and a Patchouli stem extract, in particular an extract from exhausted Patchouli stems. This composition provides anti-oxidant, anti-elastase, anti-hyaluronase, and anti-tyrosinase activities.

PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and / or increased activity, are provided. Also provided are compositions and formulations and uses thereof.

The present application relates to a novel hyaluronase variant having improved stability and a pharmaceutical composition containing the same. The present application discloses novel PH20 variants or fragments thereof, human hyaluronidase having improved thermal stability and enzymatic activity wherein the hyaluronidase is an enzyme that hydrolyzes hyaluronic acid, in particular to PH20 variants or fragments thereof comprising one or more amino acid residue substitutions in a variant having the amino acid sequence of SEQ ID NO: 3, wherein one or more amino acid residues are selectively deleted at the N-terminus and / or C-terminus.

The present invention relates to a pharmaceutical composition including (a) a drug and (b) a human PH20 variant. The human PH20 variant included in the pharmaceutical composition according to the present invention comprises amino acid residue substitution(s) in one or more regions selected from an alpha-helix 8 region (S347 to C381) and a linker region (A333 to R346) between alpha-helix 7 and alpha-helix 8 in wild-type human PH20 having the amino acid sequence of SEQ ID NO: 1, wherein amino acid residue(s) located at the N-terminus or the C-terminus is(are) selectively truncated. In addition, the pharmaceutical composition according to the present invention may further comprise a pharmaceutically acceptable additive, particularly a stabilizer. The pharmaceutical composition according to the present invention can maximize the therapeutic effect of a drug used in combination therewith, due to the effect of human PH20 variants.

The present application belongs to the technical field of daily cosmetics, and particularly relates to a vitamin E moisturizing skin cream added with peony fresh flower stock solution and a preparation method thereof. The moisturizing skin cream provided by the present application comprises, in weight parts, 1-10 parts of peony fresh flower stock solution fermentation liquor, and 0.1-2 parts of vitamin E, wherein the peony fresh flower stock solution fermentation liquor is obtained by two-step fermentation of peony fresh flower stock solution with a compound microbial agent of two-split yeast, Pseudoalteromonas and galactose yeast-like bacteria in sequence. The product obtained by compounding the vitamin E cream with the fermented peony stock solution fermentation liquor has good moisturizing property, improves the side effect of skin dryness caused by long-term use of vitamin E cream, and has good anti-allergy effect, and the prepared moisturizing cream has an inhibition rate of hyaluronidase of more than 80%.