67 results about "Peptide structure" patented technology

The invention belongs to the technical field of organic synthesis, and particularly discloses an ammonia aromatic alkene modified near-infrared BODIPY fluorescent probe as well as a preparation method and application thereof.The near-infrared BODIPY fluorescent probe is obtained by adopting 6-(dimethylamino) nicotinic aldehyde to directionally modify the alpha position of BODIPY and constructing an expanded conjugated system through Knoevenagel condensation. Wherein dimethylamino provides a strong electron donating effect, a molecular structure is twisted into a non-planar configuration due to steric hindrance of a pyridine ring, pi-pi accumulation is reduced, and an ACQ effect is inhibited. According to the structural modification strategy, significant red shift of absorption / emission wavelength is realized by expanding a molecular pi-conjugated skeleton, and meanwhile, the stability of the BODIPY core and the quantum yield are effectively enhanced. The probe molecule can specifically recognize the diphenylalanine dipeptide structural unit by utilizing the synergistic coordination effect of the 2-aminopyridine derivative and the BODIPY mother nucleus, so that the selective detection of the Alzheimer's disease biomarker Abeta42 is realized, and a novel optical detection tool is provided for the early molecular diagnosis and targeted therapy of AD (Alzheimer's disease).

The invention discloses a yak bone collagen peptide for improving osteoporosis and preparation and application thereof, and relates to the field of biological medicine, the yak bone collagen peptide comprises collagen peptide with the molecular weight of 500-2000 Daltons, contains a glycine-proline-hydroxyproline tripeptide core structure, can promote osteoblast differentiation and inhibit osteoclast activity, and can be used for treating osteoporosis. The amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine, and the amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine; and the amino acid is glycine, proline, hydroxyproline, serine, leucine and alanine. The yak bone collagen peptide has a unique glycine-proline-hydroxyproline tripeptide structure, the molecular weight is controlled within the range of 500-2000 Daltons, the structure can promote osteoblast differentiation and inhibit osteoclast activity at the same time, and the problem that a traditional osteoporosis treatment medicine is single in function is solved.

The invention provides a hair care composition with oil control, hair loss prevention and repair effects and a preparation method of the hair care composition, and belongs to the technical field of hair care. The hair care composition comprises functional peptides such as myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 and the like which are synergistically combined according to a specific proportion, and glycerin, water and a penetration enhancer are supplemented to form a basic system. By optimizing peptide structure modification, adding proportion and the steps of pH control, ultrasonic treatment and collaborative assembly in the preparation process, multi-path collaborative regulation and control of hair follicle activation, scalp inflammation inhibition and keratin synthesis enhancement are realized.

The invention provides an antibacterial peptide and application thereof in resisting bacteria and / or enhancing the disease resistance of plants. Specifically, on one hand, the invention provides two antibacterial peptides which are novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4, sequences of the novel functional antibacterial peptides ZJUAMP3 and ZJUAMP4 are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the antibacterial peptides ZJUAMP3 and ZJUAMP4 both have broad-spectrum antibacterial activity, have good antibacterial activity on bacteria such as gram-negative bacteria, gram-positive bacteria and fungi, and are suitable for scenes needing sterilization or fungus and bacterium growth inhibition. Besides, the ZJUAMP4 can activate an MAPK signal channel in a plant body and induce the plant to generate systematic disease resistance, and is suitable for the fields of crop disease prevention and control and plant protection. The antibacterial peptide is simple in structure and easy to synthesize.

This invention discloses a therapeutic molecule, nanoparticles, and their preparation method for locating and stabilizing atherosclerotic plaques. The therapeutic molecule is synthesized based on cholanonic acid and copper peptide, and the nanoparticles are formed by the self-assembly of the therapeutic molecule. The preparation method involves aldehyde modification of both ends of cholanonic acid and coupling it with copper peptide via a Schiff base reaction to obtain a therapeutic molecule with both diagnostic and therapeutic functions. The copper peptide structure in the therapeutic molecule serves as the hydrophilic segment, and the cholanonic acid structure serves as the hydrophilic segment, allowing it to further self-assemble into nanoparticles in water. These nanoparticles exhibit acid responsiveness and can disintegrate in the slightly acidic environment of atherosclerotic sites. The released copper peptide can stabilize plaques by promoting collagen secretion and inhibiting matrix metalloproteinase expression. Simultaneously, the X-ray imaging characteristics of cholanonic acid can be used to locate and diagnose the distribution of plaques, thus achieving integrated stabilization and localization of atherosclerotic plaques.

This invention relates to the field of bioinformatics processing and discloses a method and system for mining the structure-activity relationship (SMR) of porcine neurotrophic peptides based on sequence features. The method includes constructing an original sample index table and fusing multi-source production data, extracting peptide sequence features to generate a sequence feature matrix, constructing a sequence-process joint graph containing peptide nodes and process state nodes, training a structure-activity relationship graph neural network to mine SMR relationships, and deriving a process control decision table based on a process response sample set generated by the network, thereby achieving online optimization of the porcine neurotrophic peptide preparation process. This invention solves the problem of SMR mining caused by the separation of process parameters, sequence information, and activity data, achieving accurate characterization of the synergistic effect of sequence and process and reverse optimization of process parameters, thus improving the targeted enrichment efficiency and bioactivity retention level of target neurotrophic peptides.

Provided are multivalent D-peptide compounds that specifically bind to a protein of interest. The multivalent D-peptide compounds may include two or more different variant D-peptide domains linked via a linking component. The D-peptide compounds may include a plurality of different domains that specifically bind to different binding sites on a protein of interest to provide high affinity binding to and potent activity against the protein of interest. Also provided are D-peptide variant GA domain polypeptides and D-peptide variant Z domain polypeptides having a Specific Determining Motif (SDM) for specific binding to a protein of interest, such as VEGF-A or PD-1. In some embodiments in which the protein of interest is a homodimeric protein of interest (e.g., VEGF-A, PD-1), the D-peptide compounds may be similarly dimeric and include dimers of multivalent (e.g., divalent) D-peptide compounds. Methods of using the compounds are provided, including methods of treating a disease or condition associated with a protein of interest in a subject.

This invention provides modified antimicrobial peptides, belonging to the field of antimicrobial peptide technology. The modified antimicrobial peptides include at least one of a first, second, third, fourth, fifth, and sixth antimicrobial peptide; the amino acid sequences of the first, second, third, fourth, fifth, and sixth antimicrobial peptides are shown sequentially as SEQ ID NO.1 to SEQ ID NO.6. Based on prevotellin-2, the modified antimicrobial peptides were obtained by reducing negatively charged amino acids, increasing positively charged amino acids, introducing hydrophobic amino acids, adjusting or deleting amino acid sequences, and optimizing the peptide structure. In vitro minimum inhibitory concentration (MIC) determination showed that, compared with the initial peptide, the modified antimicrobial peptides exhibited significantly enhanced antimicrobial activity against bacteria and fungi, and also showed significant bactericidal activity against clinically resistant bacteria.

Provided is a compound that includes a peptide structure represented by Formula (p1) or (p2). Also provided is a method for producing droplets using the compound. Xa is an amino acid residue having a hydrophilic side chain; Xb is an amino acid residue having a side chain represented by Formula (b1); Xc and Xd are each independently an amino acid residue or a glycine residue having a hydrophobic side chain; and n is an integer of 1 to 3. Ar1 is an aryl group or heteroaryl group which may have a substituent; and Ar2 is an arylene group or a heteroarylene group which may have a substituent.

This invention discloses a method for screening pancreatic lipase inhibitory peptides based on machine learning, relating to the fields of bioinformatics and food science. The method includes the following steps: dataset construction and three-dimensional peptide structure characterization, statistical-based feature screening, outlier removal based on a dual-model consensus mechanism, feature recursive elimination and physicochemical significance optimization, data augmentation based on Gaussian noise injection, and final prediction model construction. This invention effectively solves the problems of insufficient feature representation, high noise interference, and weak model generalization ability in existing screening methods through multi-dimensional feature characterization, rigorous outlier cleaning, and data augmentation strategies for small samples. It can quickly and accurately screen highly active pancreatic lipase inhibitory peptides from massive peptide sequences, providing an efficient computational tool for the development of functional foods and lipid-lowering drugs.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regular clustering interval short palindromic repeat (CRISPR). Specifically, the invention provides the application of the chromosome regulating peptide structural domain in improving the Cas protein editing efficiency, and the chromosome regulating peptide structural domain has a wide application prospect.

This disclosure provides peptides which have an affinity for the focal adhesion targeting (FAT) domain of focal adhesion kinase (FAK). In particular, the peptides are modified and derived from the sequence of the LD2 alpha helical domain of paxillin (e.g., LD2 peptides), the LD4 domain of paxillin (e.g., LD4 peptides), and CD8 peptides. These peptides are capable of blocking an interaction between paxillin and FAK, thereby inhibiting FAK activity related to FAK-paxillin interaction. The invention further provides uses for such peptides as therapeutics for the treatment of cancer and other diseases characterized with FAK activity and / or expression (e.g., fibrosis).

The present disclosure provides a method for biocatalytic production of a polycyclic peptide comprising providing a peptide sequence containing a recognition motif for a radical S-adenosylmethionine metallocnzyme (SAM enzyme), exposing the peptide sequence to an enzymatic reaction including catalysis under standard enzymatic reaction conditions with the radical SAM enzyme, and generating a further cyclized peptide by modifying a product of the enzymatic reaction chemically and / or via enzymes. The radical SAM enzyme catalysis generates macrocyclic modifications using unactivated carbon centers. The product of the enzymatic reaction may be modified using haloalkane- or haloacetyl-containing compounds, macrocyclases, or additional radical SAM enzymes. The peptide sequence may be altered at permissive sites to generate libraries of polycyclic peptide structures. The enzymatic reaction may occur at ambient temperature and pH under buffered aqueous conditions.

The present invention relates to a peptide structure specifically binding to carbonic anhydrase IX (CAIX), and a use thereof. The CAIX-binding peptide ligand of the present invention contains D-amino acids, and thus has high binding specificity to CAIX while being stable in the body, and a cyclic CAIX-binding peptide structure comprising same can bind to CAIX with high affinity in the body, and thus is effective in the diagnosis, prevention, suppression or treatment of diseases mediated by CAIX.

[Problem] To provide: a macrocyclic compound which includes an oligoamino acid or an oligo-peptide, and which has been conventionally difficult to synthesize; and a method for producing the same. [Solution] The present inventors have newly discovered that, by reacting a dicarboxylic acid compound (diester compound) having amino acid residues at both ends of a spacer with a diamine acid compound having amino acid residues at both ends of a spacer, it is possible to synthesize a macrocyclic compound which includes an oligoamino acid or an oligopeptide in a molecule and in which the type and / or the composition ratio of amino acids can be changed.

The application discloses a branched antibacterial peptide with double fatty acid tails and application thereof, and has the structure of taking Lys as a branched core, using Arg and Trp as two kinds of amino acids to replace the C-terminal of Lys, e -amino, α -amino, to obtain a branched peptide structure. The same length of saturated fatty acid tail chains are introduced into two N-terminal ends of the branched peptide structure to obtain the branched antibacterial peptide with double fatty acid tails, and the structure general formula is Cn-Z-K(Y-Cn)-X, which is marked as XYZn, wherein K is Lys, X, Y and Z are end-point amino acids Arg or Trp, Cn is a saturated fatty acid chain, and n is the number of carbon atoms of the fatty acid chain. The antibacterial peptide structure is novel, simple to synthesize, and has the advantages of broad-spectrum antibacterial activity, low toxicity and high safety in in-vitro antibacterial experiments and toxicity tests. Serum stability experiments show that the antibacterial peptide has excellent stability in mouse serum, and acute toxicity experiments show that the antibacterial peptide has high safety. Therefore, the antibacterial peptide has a good application prospect in preparation of clinical antibacterial drugs.

There is provided a method for producing a peptide-nucleic acid complex containing a peptide and a nucleic acid encoding the peptide. The method for producing a peptide-nucleic acid complex includes a step of preparing a nucleic acid to which a transpeptidase N-terminal substrate motif has been added, the nucleic acid containing a first coding sequence encoding a peptide, a second coding sequence encoding a transpeptidase, and a third coding sequence encoding a transpeptidase recognition motif; a step of synthesizing a chimeric protein containing a domain of the peptide, a domain of the transpeptidase, and the transpeptidase recognition motif, from the nucleic acid to which the transpeptidase N-terminal substrate motif has been added, using a cell-free protein synthesis system; and a step of forming the peptide-nucleic acid complex by means of the transpeptidase domain.

The invention provides a homologous recombinant expression vector for expressing a Pt5-1c active peptide in dunaliella salina, the provided expression vector can efficiently express an exogenous gene in dunaliella salina, and the homologous recombinant expression vector comprises a construction component which comprises a promoter with a nucleotide sequence of SEQ ID NO: 1. According to the constructed expression vector, an exogenous gene is separated from other genes through a P2A sequence, so that the exogenous gene is independently expressed, and interference among the genes is avoided; an iron oxidase signal recognition sequence is introduced while an exogenous gene is inserted, so that active peptides such as Pt5-1c and the like can be guided to be secreted out of cells, and the problems of impurity release, active peptide structure damage and the like caused by obtaining the active peptides by breaking the cells are avoided. According to the constructed expression vector, an enterokinase enzyme cutting site is introduced, so that the active peptide can be conveniently cut and separated in the subsequent separation and purification process, the consumption of reagents and consumables is reduced, the purification efficiency is improved, and the purification cost is reduced.

The application relates to an anticancer peptide prediction method and system based on feature fusion and cross attention mechanism. The method comprises the following steps: constructing a data set containing various protein sequences; inputting the protein sequence into a protein language model ESM-2 to extract peptide structure features; inputting the protein sequence into a feature extraction model to extract peptide physical and chemical features; performing dimension transformation processing on the peptide structure features, using BiLSTM to continuously process discrete peptide physical and chemical features; using a cross attention mechanism for feature fusion to obtain target features input into a multilayer perception machine (MLP) to obtain an anticancer peptide prediction result. By using a protein language model to extract peptide structure features, using a traditional feature extraction model to extract peptide physical and chemical features, and using a cross attention mechanism for feature fusion, time-consuming and high cost can be avoided, and the extracted features are related to each other, so that anticancer peptide prediction can be quickly, efficiently and accurately performed.

The invention discloses a mirror symmetry antibacterial oligopeptide rich in tryptophan and arginine and application of the mirror symmetry antibacterial oligopeptide. The antibacterial oligopeptide is obtained in the mode that tryptophan W and arginine R form a mirror symmetry peptide structure, then hydrophobic amino acid is modified at the two ends of the peptide sequence, and amidation is conducted on the C tail end; the structural general formula of the fluorescent probe is XRnWmRWmRnX-NH2, and the fluorescent probe is marked as RXm, or XWnRmWRmWnX-NH2, which is marked as WXm; or X (WR) 3WX-NH2, which is marked as RX; or X (RW) 3RX-NH2, which is marked as WX; wherein n = 0, 1 or 2, m = 1, 2 or 3, and n + m = 3. An in-vitro antibacterial activity experiment and a hemolysis experiment show that the antibacterial oligopeptide has relatively strong antibacterial activity on gram-positive bacteria and gram-negative bacteria, and meanwhile, the antibacterial oligopeptide has low hemolysis toxicity. The optimal antibacterial oligopeptide WF is obtained by calculating the therapeutic index, the corresponding full D-type amino acid sequence wf is synthesized, and the wf has high-efficiency antibacterial activity and low toxicity and also has high enzymolysis stability. Therefore, the antibacterial oligopeptide provided by the invention has a good application prospect in preparation of clinical antibacterial drugs.

The invention provides an oligonucleotide hydrogel micro-nano gene compound as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: firstly, designing C5ASO, and loading the C5ASO on a nano-carrier modified with HAP-1 peptide (targeting fibroblast-like synovial cells) and active ester; meanwhile, the porous HAMA hydrogel microspheres are prepared by utilizing a photo-crosslinking micro-fluidic technology; then, DBCO-TIMP and an azide group are respectively introduced into the liposome and the hydrogel microspheres through Michael addition and an amide coupling reaction, so that a micro-nano gene compound (C5ASO (at) HAP-CL-TIMP (at) HMs) is formed; in addition, the liposome and the hydrogel microspheres are efficiently compounded through click chemistry. The hydrogel micro-nano gene compound not only can efficiently load ASO lipidosome, but also can enhance the stability of a gene therapeutic agent in an in-vivo delivery process. Meanwhile, the MMP-9 response peptide structure in the RA articular cavity inflammation microenvironment can be accurately activated, so that continuous complement intervention is realized.

The invention discloses a double-target-point cyclic peptide and a synthesis method and medical application thereof.The amino acid sequence of the double-target-point cyclic peptide is Cys-Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Alaa-Cys-Lys-Pro-Ser-Trp-Arg-Alaa-Asp, Cys1 and Cys10 form a cyclic peptide structure through disulfide bond cyclization, the relative positions of the N terminal and the C terminal are stabilized, the enzyme resistance and the structural stability are improved, and the double-target-point cyclic peptide has the advantages that the double-target-point cyclic peptide is obtained; further, protease degradation is resisted, the half-life period is prolonged, and efficient analgesia is realized by synergistically activating a mu opioid receptor and inhibiting a voltage-gated sodium ion channel 1.8. The double-target cyclopeptide designed by the invention has the beneficial characteristics of simple structure, convenience in artificial synthesis, low production cost and the like, and has huge medical industrial applicability.

Compounds for the detection of the activity of a protease include a recognition domain / substrate having an amino acid or peptide structured to interact with the protease, a reporter molecule, and a linking group forming a covalent bond between the recognition domain and reporter molecule, wherein upon interaction of the recognition domain with the protease, the covalent bond is destroyed, rendering the reporter molecule detectable by a chemical detection device. Methods of detecting enzymatic activity of a single protease may include reacting a compound as presented herein with a protease, and identifying detectable reporter molecules with a chemical detection device. Methods of detecting enzymatic activity of a plurality of proteases may include reacting a mixture of compounds, as presented herein, that are cleaved by proteases (optionally, orthogonal proteases) and release distinct reporters with proteases, and identifying detectable reporter molecules with a chemical detection device.

The present invention provides a novel ring-closing metathesis reaction for constructing a peptide structure having a medium ring. The present invention provides a method for performing the ring-closing metathesis reaction, wherein the ring-closing metathesis reaction is carried out at a low dilution condition (for example, a concentration of 0.1 mol / L). The present invention provides a method for performing a ring-closing metathesis reaction suitable for mass production.

The invention relates to the field of medicinal chemistry, and relates to a small molecule peptide for inhibiting growth of staphylococcus, and an analogue and application thereof. The method comprises the following steps: finding a rhodococcus genome from an NCBI (National Center of Biotechnology Information) microbial genome database, and predicting a non-ribosome peptide structure synthesized by a non-ribosome peptide synthetase (NRPS) sequence through software such as AntiSMASH and Prism; the method comprises the following steps: classifying all NRPS biosynthetic gene clusters (BGCs) through Bigscape to obtain a gene cluster family (GCFs); the method comprises the following steps: predicting the selectivity of a GCFs substrate and screening a polypeptide with antibacterial potential through Phammapper; a small molecule peptide (compound I) is obtained through chemical synthesis, and the small molecule peptide is found to have an inhibition effect on staphylococcus epidermidis and staphylococcus pseudointermedius; afterwards, alanine is used for replacing glutamine to increase hydrophobicity of glutamine (compound II), the antibacterial effect of the compound II is obviously enhanced, and the compound II has the potential of developing novel antibacterial drugs and biopesticides.

The application belongs to the technical field of skin care product raw materials, and discloses a kind of short peptide compounds and preparation method, cosmetic composition and application.The short peptide compound has structural formula: the short peptide compound provided in the application contains peptide segment of tyrosine (Tyr), beta-alanine (beta-Ala), cysteine (Cys) and proline (Pro) and other antioxidant amino acids, these amino acids can directly participate in the removal of free radicals or enhance the antioxidant effect by forming a stable peptide structure, so as to obtain a short peptide compound with antioxidant and physiological repair effect.Meanwhile, the mild Fmoc route is used in the preparation method, the condensation reaction time of each step is short, the production cycle is greatly shortened, the raw material source is convenient, the process is stable, the quality is controllable, the product yield and purity are high, and the production can be scaled up.

The invention belongs to the technical field of bioactive peptide preparation and food processing, and particularly relates to a giant salamander muscle peptide zinc chelate cranberry juice beverage and a preparation method thereof. Giant salamander peptide is extracted from giant salamander muscle, zinc ions are introduced for chelation, the giant salamander muscle peptide zinc chelate is prepared, finally, the giant salamander muscle peptide zinc chelate is compounded with cranberry juice, and then the giant salamander muscle peptide zinc chelate cranberry juice beverage is developed. Compared with a common fruit juice beverage or a single peptide functional beverage, the giant salamander muscle peptide zinc chelate cranberry juice beverage prepared by the preparation method disclosed by the invention is rich in nutritional value, unique in flavor and mouth feel, green and healthy in formula and wide in product application scene, the chelate is high in stability in a beverage system, and the retention rate of zinc is high after high-temperature sterilization and normal-temperature storage; and the product has stable quality in the shelf life, and is suitable for large-scale industrial production.

Provided herein are methods, devices, and kits for identifying glycosylated polypeptide biomarkers and signatures for progression of a disease or a condition, such as cancer or NASH, or and response of the disease or condition to a treatment. Also provided herein are: i) methods of generating and analyzing glycosylated polypeptide biomarkers, ii) methods of validating a model using glycosylated polypeptides for predicting the disease or condition or for making treatment recommendation, iii) systems and methods for implementing QC of a cohort of samples by analyzing peptide structure data for each sample using a machine learning model to generate a predicted age and / or sex associated for each sample. The quality control issue may include an error of mislabeled samples or an error from sample preparation, or a systemic measurement or an instrument error.

A method and system for diagnosing a subject with respect to a pancreatic cancer disease state. Peptide structure data corresponding to a biological sample obtained from the subject is received. The peptide structure data is analyzed using a supervised machine learning model to generate a disease indicator that indicates whether biological sample evidences the PC disease state based on at least 3 peptide structures selected from a group of peptide structures of Group I identified in Table 1 or of Group II of Table 8. The group of peptide structures in Table 1 or Table 8 comprises a group of peptide structures associated with the PC disease state. The group of peptide structures is listed in Table 1 with respect to relative significance to the disease indicator. A diagnosis output is generated based on the disease indicator

The objective is to provide a collagen production promoter that can efficiently promote collagen production. [Solution] A collagen production promoter containing a 3-hydroxypyridinium derivative represented by the following general formula (1) as an active ingredient. TIFF2026076348000020.tif49159 In the formula, A1 independently represents a hydroxyl group, an alkoxy group having 1 to 10 carbon atoms, a peptide structure having 1 to 100 amino acids linked at the N-terminus, a hydrogen atom, an amino group, or a hydrocarbon group having 1 to 10 carbon atoms, while A2 independently represents a hydrogen atom, a hydrocarbon group having 1 to 10 carbon atoms, an acyl group, or a peptide structure having 1 to 100 amino acids linked at the C-terminus.