166 results about "Basic amino acids" patented technology

The invention discloses basic amino acid modified 3S-PCL as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The basic amino acid modified 3S-PCL has a structural formula shown in the specification, wherein R is alkaline amino acid; m, m1 and m2 are all positive integers, and m, m1 and m2 are greater than or equal to 1. According to the invention, a basic amino acid modified three-arm polycaprolactone (3S-PCL) material is designed and synthesized, and the basic amino acid modified three-arm polycaprolactone (3S-PCL) material has good biocompatibility and can be used for lipid nanoparticle nucleic acid delivery instead of a cationic liposome. The preparation method of the basic amino acid modified 3S-PCL material is simple and rapid, and is especially suitable for industrial production. The lipid nanoparticles prepared by using the basic amino acid modified 3S-PCL material as a gene vector can realize efficient delivery of nucleic acid drugs.

The present disclosure provides daptomycin formulations which have improved chemical stability, faster reconstitution times when reconstituted from the solid state and longer in-use stability. The compositions comprise daptomycin, one basic amino acid or its salt and optionally one or more pH adjusting agents.

The invention discloses an aspergillus oryzae engineering bacterium as well as a construction method and application of the aspergillus oryzae engineering bacterium, and relates to the technical field of genetic engineering. The method comprises the following steps of: (a) connecting and fusing an open reading frame of an endogenous gene with a fungal laccase copper oxidoreductase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; or connecting and fusing an open reading frame of an endogenous gene with a multi-copper oxidase structural domain and a target heterologous protein gene through a dual-basic amino acid endopeptidase cleavage site gene; and (b) at least one of a gene Aovps10, a gene AosedD and a gene Aoemp47 is knocked out. The aspergillus oryzae engineering bacterium provided by the invention can realize high-efficiency expression of the heterologous protein, and the extracellular yield of the heterologous protein is obviously improved.

Provided is a nanovaccine. The nanovaccine is prepared from the following starting materials: a sustained-release carrier, an antigen, an adjuvant, and a cationic component. The mass ratio of the sustained-release carrier to the antigen to the adjuvant to the cationic component is (1-30):1:(0.1-1):(0.05-0.5). The sustained-release carrier comprises sodium alginate. The antigen comprises at least one of a protein, a recombinant subunit, and a polypeptide. The cationic component comprises at least one of a calcium ion, a manganese ion, an aluminum ion, a polyamine, a basic amino acid, and a polyamino acid consisting of 2-30 basic amino acids. The nanovaccine comprises the sustained-release carrier sodium alginate, the antigen, the adjuvant, and the cationic component in a proper ratio. Sodium alginate is used as a sustained-release carrier and is promoted to form a network structure under the action of the cationic component, so as to encapsulate the antigen and the adjuvant, thereby forming a stable antigen-carrier-adjuvant nanoparticle gel. The nanovaccine can induce a stronger immune response and has good stability and good biocompatibility.

The present invention discloses a cyclic lipopeptide carrier for encapsulating nucleic acid drugs, comprising a cyclic lactone structure formed by a heptapeptide R1R2R2R3R1R2R2 and a β-hydroxy fatty acid with a carbon chain length of 6 to 44, and its general structural formula is as follows: #imgabs0# wherein R1 is a basic amino acid, including one of histidine His, lysine Lys or arginine Arg, R2 is one of the hydrophobic amino acids leucine Leu, isoleucine Ile or valine Val, and R3 is one of any amino acid; n is an integer of 2 to 40; the positively charged basic amino acids in the cyclic lipopeptide bind to the negatively charged nucleic acid drug through electrostatic interaction to form cyclic lipopeptide-nucleic acid particles. The cyclic lipopeptide carrier for encapsulating nucleic acid drugs provided by the present invention has high safety, can effectively protect nucleic acid drugs, improves their stability, and has good transfection efficiency for a variety of cells, and has broad application prospects in the field of nucleic acid drug delivery.

Compounds and methods are provided for inhibiting a PI4-kinase. Methods of treating a pathogen infection and methods of treating cancer are also provided. The PI4-kinase inhibitor can be a compound that is a 5-aryl or heteroaryl-thiazole, e.g., as described herein. In certain embodiments, the PI4-kinase inhibitor is a substituted 2-amino-5-phenylthiazole or substituted 2-amino-5-pyridylthiazole compound. In some embodiments, the compounds have broad spectrum anti-infective activity against a variety of infective diseases, where the diseases are caused by pathogens containing a basic amino acid PIP-2 pincer (BAAPP) domain that interacts with phosphatidylinositol 4,5-bisphosphate (PIP-2) to mediate pathogen replication. Also provided are methods of treating a subject for cancer using a PI4-kinase inhibitor. Aspects of the methods include inhibiting PI4-kinase in a cancer cell to reduce cellular proliferation.

The invention belongs to the technical field of biological medicine, and particularly relates to hyaluronic acid nano-microspheres as well as a preparation method and application thereof. According to the preparation method of the hyaluronic acid nano-microspheres, the hyaluronic acid nano-microspheres are prepared through interaction of nucleic acid, basic amino acid and hyaluronate in a specific proportion, the preparation process is optimized, and the prepared hyaluronic acid nano-microspheres are high in hyaluronic acid adding amount, high in skin permeability and good in skin care effect. The stability is good, and the application requirements in transdermal drug delivery drugs, skin care products and other products are met. Meanwhile, a non-covalent binding mode is adopted for preparing the nano-microspheres, the preparation operation is simple, the gelling property of the hyaluronate is effectively improved, the sticky feeling under high content is improved, and the application scene of hyaluronic acid in the field of skin care products is widened.

The present application relates to: a culture medium composition for microorganisms of Corynebacterium species, in which part of ammonium sulfate is replaced with a different ammonium-containing compound; and a method for producing a basic amino acid by using the culture medium composition.

The application discloses a preparation method of an immune protein extract of colostrum with tumor inhibition efficacy, and comprises the following steps: (1) raw material selection; (2) flocculation of casein; (3) sugar removal; (4) ultrafiltration membrane filtration; (5) directional hydrolysis; (6) cation exchange resin adsorption; (7) desalination; and (8) freeze drying. The extract of the application is rich in hydrophobic amino acid and alkaline amino acid hydrolysis fragments of the immune protein of colostrum, and has stronger antioxidant, immune enhancement and tumor inhibition activities. Different from common immune proteins of colostrum, the extract plays the anti-tumor activity as a result of the combined action of its double effects of immune enhancement and tumor cell growth inhibition.

The present invention provides: a Hyal1 variant in which, in wild-type human hyaluronidase Hyal1 comprising glutamic acid 131 as a catalytic amino acid, at least one amino acid among amino acids adjacent to the catalytic amino acid, i.e., glutamic acid 131 in primary and tertiary structures is substituted with an acidic, polar or basic amino acid; a method for producing the Hyal1 variant; a nucleic acid applicable to the production of the Hyal1 variant; an expression vector; a host cell; and a Hyal1 variant preparation or use thereof. The hyaluronidase Hyal1 variant of the present invention can effectively degrade hyaluronic acid not only at acidic pH but also at neutral pH, and thus is highly applicable.

The present invention discloses a method for preparing a nickel oxide thin film, its product and application, belonging to the technical field of photovoltaic materials and devices. The preparation method of the present invention includes: providing a homogeneous aqueous solution containing a nickel source compound and a basic amino acid, and chemically bath-depositing the homogeneous aqueous solution on the surface to be coated of a substrate, so that a nickel hydroxide film layer is formed in-situ on the surface to be coated, and then annealing the nickel hydroxide film layer to generate a nickel oxide thin film. By introducing a basic amino acid into the nickel source chemical bath aqueous solution for chemical bath deposition, the present invention can not only effectively and reasonably adjust the nucleation rate of nickel hydroxide by forming multi-dentate coordination with moderate strength between the basic amino acid and nickel ions, but also prevent the aggregation of nanoparticles through steric hindrance effect, effectively improving the dispersion effect of nanoparticles. Thus, the deposition behavior of nickel hydroxide nanoparticles is synergistically optimized in two aspects, and finally the controllable preparation of a nickel oxide thin film with uniform density and good crystallinity is achieved.

This invention discloses a cyclic lipopeptide delivery system, comprising a cyclic lipopeptide carrier and nucleic acid; the head of the cyclic lipopeptide carrier is composed of heptapeptides R1R2R2R3R4R2R2 linked together by amide bonds to form a ring, and the hydrophobic tail of the cyclic lipopeptide carrier is composed of a fatty acid with a carbon chain length of 6-44 forming an ester bond with the hydroxyl group on the head amino acid R3, with the following general structural formula: where R1 is a basic amino acid, including one of histidine (His), lysine (Lys), or arginine (Arg); R2 is a hydrophobic amino acid, including one of leucine (Leu), isoleucine (Ile), or valine (Val); R3 is a hydrophilic amino acid, including one of tyrosine (Tyr), serine (Ser), or threonine (Thr); R4 is one of glutamate (Glu) or glutamine (Gln); n is an integer from 5 to 43; the cyclic lipopeptide carrier encapsulates the nucleic acid drug to form cyclic lipopeptide-nucleic acid nanoparticles.

Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and / or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

This invention discloses a method for preparing a Schiff base corrosion inhibitor for AZ91D magnesium alloy coolant. The method involves adding an ethanol or acetone solution of the ketone-containing heterocyclic compound dropwise to an aqueous solution of the alkaline amino acid at a molar ratio of 1:1 to 1.5. The reaction is carried out at 40–80°C for 4–36 hours, followed by concentration, extraction with glacial ethyl acetate, washing with glacial ethyl acetate, and drying to obtain the corrosion inhibitor. Further, the corrosion inhibitor is formulated into a coolant specifically for AZ91D magnesium alloy. This coolant is composed of a mixture of raw materials with the following weight percentages: 0.1–2% corrosion inhibitor, 39–60% deionized water, and 39–60% ethylene glycol. This invention features a simple preparation process, and the obtained corrosion inhibitor can be stored for a long time, exhibits good thermal stability, strong oxidation resistance, low conductivity, high corrosion inhibition efficiency, and good corrosion inhibition effect on AZ91D magnesium alloy.

The invention relates to the technical field of biological detection, and discloses a sample diluent and application thereof and a kit, pure water is taken as a solvent, and the sample diluent at least comprises 15-80 g / L of soluble sodium salt, 0.05-0.5 g / L of soluble potassium salt, 8-15 g / L of amino acid, 0.2-1 g / L of goat serum albumin, 0.2-1 g / L of surfactant, 0.5-2 g / L of trehalose and 0.5-2 mL / L of Proclin-300 according to concentration, the sample diluent provided by the invention can weaken non-specific binding of impurities in a blood sample and a target analyte, so that the accuracy of a final detection result is improved.

The invention relates to a fertilizer comprising at least one basic L-amino acid, for example arginine or lysine, the majority of the basic L-amino acid contained being present as its monophosphate. The alkaline L-amino acid phosphate may be combined with a binder and / or provided with an outermost layer as a coating. The invention also relates to a method of promoting plant growth by using a basic L-amino acid phosphate for a plant.

The invention discloses dark baked barley rich in wheat fragrance as well as a preparation method and application of the dark baked barley, and belongs to the technical field of wheat making. Comprising the following steps: selecting barley of which the basic amino acid content is more than or equal to 30mg / L and the lignin content is less than or equal to 100mg / g as The barley baking method is characterized in that barley is baked in a sectional manner, and the barley baking method comprises the following steps: in the first stage, the barley is heated to 50-55 DEG C at a constant speed, and the water content of the barley is 12-15%; in the second stage, the temperature is rapidly increased to 90 DEG C, and the water content of the barley is reduced to 8-10%; in the third stage, the baking temperature continues to be increased to 210-220 DEG C, and baking is conducted for 20-30 min; in the fourth stage, when the crispness of the baked barley reaches 98% or above, the baking temperature is lowered to 190-210 DEG C, and then the baked barley is rapidly cooled to the room temperature. The preparation method is applied to the aspect of drinks, and solves the problems that although the color of the baked barley obtained by the existing dark-color baked barley preparation process meets the requirement, the barley fragrance cannot be highlighted due to the low pyrazine content, the smoky flavor is large due to the high guaiacol content, and the technical requirement that pure and rich barley fragrance can be obtained by extracting a small amount of the baked barley cannot be met.

The invention relates to the technical field of composite material preparation, in particular to a two-dimensional material modified epoxidized natural rubber composite material and a preparation method and application thereof. The method comprises the following steps: (1) preparing a basic amino acid modified two-dimensional material dispersion liquid; (2) preparing modified epoxidized natural rubber; and (3) mixing the alkaline amino acid modified two-dimensional material dispersion liquid with modified epoxidized natural rubber, and adopting a wet chemical method to obtain the two-dimensional material modified epoxidized natural rubber composite material. According to the invention, not only is the dispersibility of the two-dimensional material in the epoxidized natural rubber improved, but also the composite material is endowed with excellent conductivity, and the electromagnetic shielding and mechanical properties of the composite material are achieved at the same time; the problems of contradiction between strength and toughness, reduction of ductility, contradiction between mechanical property and shielding property and the like of an epoxidized natural rubber-based composite material in the prior art are effectively solved, and the composite material is suitable for industrial production.

The application discloses a preparation method of a nano-composite enzyme for detecting tannic acid, and the method comprises the following steps: step one, adopting alkaline amino acids and polypeptides with imidazole groups to simulate the primary structure of natural oxidase, and preparing amino acid and polypeptide modified cerium oxide with enhanced oxidase and peroxidase performance; step two, in-situ compounding the polypeptide modified cerium oxide and zeolite imidazolate framework material with large specific surface area, good stability, hydrophilicity and hydrophobicity, and specific electron distribution to obtain nano-composite enzyme with enhanced oxidase and peroxidase performance; and step three, after etching treatment of the nano-composite enzyme in a polar medium, the nano-composite enzyme with enhanced oxidase and peroxidase performance for detecting tannic acid is obtained. By utilizing the competition between tannic acid and a substrate, the tannic acid is detected by using a colorimetric or fluorescent method, and the operation process is simple and rapid, the selectivity is high, the detection limit is low, the detection range is wide, and the nano-composite enzyme shows a good application prospect for sensitive detection of tannic acid.

To provide a hair-deforming cosmetic composition exhibiting low hair damage and having either a curling effect or a hair-straightening effect.SOLUTION: A hair-deforming cosmetic composition comprises thioglycolic acid, cysteamine, and an organic acid having a pKa value higher than that of thioglycolic acid, and its pH is adjusted to 8.0 or lower with a basic amino acid.SELECTED DRAWING: None

The invention provides a copolymer polypeptide as well as a pharmaceutical composition and application thereof. The copolymer polypeptide is formed by linear random copolymerization of a first monomer, a second monomer and a third monomer; the first monomer is selected from any one of alkaline amino acid and derivatives thereof; the second monomer is selected from any one of acidic amino acid and derivatives thereof; the third monomer is selected from any one of neutral amino acid and derivatives thereof. The copolymer polypeptide and the pharmaceutical composition thereof provided by the invention can effectively relieve hyperkeratosis caused by psoriasis, relieve inflammatory enteritis and effectively inhibit tumor growth.

This invention discloses a basic amino acid-modified 3S-PCL, its preparation method, and its applications, relating to the field of biomedical technology. The structural formula of the basic amino acid-modified 3S-PCL is as follows: [Structure formula would be inserted here]; where R is a basic amino acid; m, m1, and m2 are all positive integers, and m, m1, and m2 ≥ 1. This invention designs and synthesizes a basic amino acid-modified three-armed polycaprolactone (3S-PCL) material, which exhibits good biocompatibility and can replace cationic liposomes for nucleic acid delivery via lipid nanoparticles. The preparation method of this basic amino acid-modified 3S-PCL material is simple and rapid, particularly suitable for industrial production. Lipid nanoparticles prepared using this basic amino acid-modified 3S-PCL material as a gene carrier can achieve highly efficient delivery of nucleic acid drugs.

A hair cleaning agent composition which has excellent foaming and foam thickening properties and suppresses yellowing over time, and which contains (A) an amino acid-type surfactant represented by general formula (1), (B) an amphoteric surfactant, (C) an anionic polysaccharide and (D) isoprene glycol, the mass ratio of the total content of component (A) and component (B) to the content of component (D) [{(A) + (B)} / (D)] being 2-8. (R1 represents a linear or branched alkyl group or alkenyl group having 5-23 carbon atoms, or a phenyl group substituted with a linear or branched alkyl group or alkenyl group having 5-23 carbon atoms, R2 represents a hydrogen atom or an alkyl group having 1-3 carbon atoms, R3 and R4 may be the same or different and represent a hydrogen atom or-(CH2) m-COOM2, m and n may be the same or different and represent 0-20, M1 and M2 may be the same or different, and m and n may be the same or different and represent 0-20. And each represents a hydrogen atom, an alkali metal, an alkaline earth metal, an alkanolamine, ammonium or a basic amino acid. > # imgabs0 #

The problem to be addressed by the present invention is to provide: a variant REP protein that can improve low productivity of an AAV vector; a rep gene that encodes the variant REP protein; an expression vector; a vector kit for producing a recombinant adeno-associated virus vector; a cell; and a method for producing a recombinant adeno-associated virus vector. The present invention provides a variant REP protein in which one or more amino acid residues from among the 102nd amino acid residue, the 114th amino acid residue, the 125th amino acid residue, and the 134th amino acid residue of the REP protein are substituted with a basic amino acid residue in the amino acid sequence of the adeno-associated virus REP protein.

The application relates to the technical field of biological detection, and discloses a sample diluent, application and kit thereof, wherein the solvent is pure water and at least includes 15-80 g / L of soluble sodium salt, 0.05-0.5 g / L of soluble potassium salt, 8-15 g / L of amino acid, 0.2-1 g / L of goat serum albumin, 0.2-1 g / L of surfactant, 0.5-2 g / L of trehalose and 0.5-2 mL / L of Proclin-300; the amino acid is an alkaline amino acid; and the sample diluent provided by the application can weaken the non-specific combination of impurities and target analytes in a blood sample, thereby improving the accuracy of a final detection result.

A method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner is disclosed. A method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein is also disclosed. It has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

A composition for treating dentin hypersensitivity, comprising: a first component comprising: (i) a first rheology modifying polymer; (ii) a calcium ion source; (iii) a positively charged basic amino acid; and (iv) an acidifying agent, wherein the first component has a pH in the range of about 2.0 to about 7.0; a second component comprising: (i) a second rheology modifying polymer; (ii) a phosphate ion source; and (iii) an alkalizing agent, wherein the second component has a pH in the range of about 7.0 to about 14.0; wherein the first component and the second component are combined into a mixture such that a cationic amorphous calcium phosphate gel composition is created instantly when the pH of the mixture reaches a pH range of about 7.0 to about 9.5.

The application relates to the technical field of biological pharmacy, in particular to an antibacterial peptide AI-SC-4 and application thereof. The antibacterial peptide AI-SC-4 is an artificial synthetic non-natural 22-peptide, the amino acid sequence is shown in SEQ ID NO:1, the theoretical molecular weight is 2700.26 Da, and the antibacterial peptide is a cationic amphiphilic polypeptide rich in basic amino acids and hydrophobic amino acids. The antibacterial peptide has the advantages of clear structure, small molecular weight and convenient artificial synthesis, and shows strong in-vitro antibacterial activity on gram-positive bacteria and gram-negative bacteria, and can be used for drug for resisting drug-resistant bacteria infection, a disinfectant, a skin external preparation and an oral care product, and has a wide application prospect.

The application belongs to the technical field of medical preparations, and particularly relates to a high-dissolution solid preparation of multitarget and a preparation method thereof. The solid preparation is composed of 5-20% of multitarget, 10-30% of alkaline amino acid, 40-70% of hydrophilic filler, 2-10% of disintegrant, 0.1-1% of metal ion complexing agent and 0.5-2% of lubricant according to percentage by weight. The multitarget and the alkaline amino acid form a co-amorphous state in a molar ratio of 1:1 to 1:3. The preparation method comprises the following steps: dissolving the multitarget and the alkaline amino acid in an organic solvent containing volatile acid, removing the solvent under reduced pressure to obtain a co-amorphous substance, mixing the co-amorphous substance with the hydrophilic filler, the disintegrant and the metal ion complexing agent, and mixing the mixture with the lubricant after dry granulation and then compressing the mixture into tablets. The application eliminates the lattice energy limitation of the multitarget, realizes supersaturated dissolution, inhibits recrystallization during storage, maintains stable dissolution curve, improves drug loading capacity, and avoids damage to the amorphous state caused by moisture and heat through dry granulation.

The invention claimed herein relates to a dielectric polishing composition and a method thereof. The invention claimed herein particularly relates to a composition comprising: (A) surface-modified colloidal silica particles comprising negatively charged groups on the surface of the particles, wherein the surface-modified colloidal silica particles have a negative charge, a particle size of 60 nm to 200 nm, and a zeta potential of < -35 mV at a pH in the range of > 2.0 to < 6.0; (B) at least one corrosion inhibitor selected from at least one guanidine derivative; (C) at least one iron (III) oxidizing agent; (D) at least one buffering agent selected from at least one basic amino acid having an isoelectric point (pi) of > 6.5; (E) at least one stabilizer; and (F) an aqueous medium, wherein the pH of the composition is in the range of > 2.0 to < 4.3, and wherein the composition has a polyacrylamide content of < 1 ppm.