93 results about "Aromatic amino acids" patented technology

The invention belongs to the technical field of catalysts, and particularly relates to a general preparation method and application of amino acid intercalated layered hydroxide. According to the catalyst material, the nickel-iron layered hydroxide is optimized through amino acid intercalation, aromatic amino acid is anchored between layers of the nickel-iron layered hydroxide through a one-step hydrothermal method, dissolution of metal ions is inhibited, the catalyst structure is stabilized, an interlayer electronic structure is regulated and controlled, and reaction kinetics is accelerated. According to the invention, the improvement method of the organic-inorganic hybrid catalyst is perfected, and a new thought is provided for the development of the nickel-iron layered hydroxide catalyst.

The embodiment of the invention provides a recombinant pichia pastoris strain as well as a construction method and application thereof. The construction method comprises the following steps: by taking a pichia pastoris strain as a host strain, constructing a dual-synthesis path of phenylalanine ammonialyase and tyrosine ammonialyase in the host strain to obtain a first engineering strain; removing chorismic acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing an aromatic amino acid synthesis route of the second engineering strain to obtain a third engineering strain; knocking out a branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing precursor supply of the fourth engineering strain to obtain a recombinant pichia pastoris strain; according to the construction method provided by the embodiment of the invention, the synthesis path of p-coumaric acid can be systematically optimized, the recombinant pichia pastoris strain for efficiently producing p-coumaric acid by taking methanol as a carbon source is constructed, and compared with a host strain, the recombinant pichia pastoris strain has the advantage that the yield of p-coumaric acid is remarkably increased.

The invention belongs to the technical field of metabolic engineering and biological manufacturing, and particularly relates to an engineering bacterium for producing gallic acid (GA) and application of the engineering bacterium in production of gallic acid. According to the method, key metabolic nodes in the gallic acid production process are systematically regulated, for example, the hydroxylase activity is improved, the pyruvic acid flow direction is regulated, the aromatic amino acid precursor supply is improved, and the dynamic balance between the engineering bacterium growth and the gallic acid product synthesis is realized, so that the gallic acid yield and the carbon yield are remarkably improved. Experiments show that the gallic acid yield of the engineering bacteria prepared by the invention reaches 12.5 g / L in a shake flask experiment, and reaches 109.8 g / L in a 3 L fermentation tank; and the carbon yield is increased to 0.64 g / g glucose, which is the highest level reported in the current literature.

The invention discloses a microbial composite flora, a microbial agent and application of the microbial composite flora and the microbial agent, and relates to the technical field of microorganisms and application of the microorganisms. The invention relates to a microbial composite flora, which is characterized in that the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12, and the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12. The microorganisms for constructing the composite flora are separated from the semi-coke wastewater, have better phenol resistance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biologically removing nitrogen and phosphorus and degrading COD. Under the condition that the original microbial community composition in the wastewater is not changed, the microbial compound flora can effectively improve the degradation rate of COD (Chemical Oxygen Demand) in the phenol-ammonia wastewater and effectively reduce the treatment cost of high-phenol-ammonia wastewater such as semi-coke and the like through the synergistic effect of the strains, and has great potential in the aspect of promoting the low-carbon green sustainable development of the coal chemical industry.

The application provides a recombinant Pichia pastoris strain and a construction method and application thereof. The construction method comprises the following steps: taking a Pichia pastoris strain as a host strain, constructing a double synthesis pathway of phenylalanine ammonia lyase and tyrosine ammonia lyase in the host strain to obtain a first engineering strain; relieving the branch acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing the aromatic amino acid synthesis pathway of the second engineering strain to obtain a third engineering strain; knocking out the branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing the precursor supply of the fourth engineering strain to obtain the recombinant Pichia pastoris strain. The construction method can systematically optimize the synthesis path of p-coumaric acid, and the recombinant Pichia pastoris strain for efficiently producing p-coumaric acid with methanol as a carbon source is constructed. Compared with the host strain, the yield of p-coumaric acid of the recombinant Pichia pastoris strain is significantly improved.

The invention provides a heat-stress-resistant laying hen feed and a preparation method thereof. The heat-stress-resistant laying hen feed comprises the following raw materials in parts by weight: 50-60 parts of corn, 10-20 parts of soybean meal, 5-15 parts of wheat bran, 1-5 parts of vegetable oil, 5-12 parts of fish meal, 2-5 parts of beta-alanine, 2-5 parts of activated black bean powder, 2-5 parts of quercitrin, 0.2-0.5 part of multivitamins, 4-8 parts of an electrolyte poising agent and 2-3 parts of compound amino acids. The activated black bean powder is obtained by performing enzymolysis activation on black bean powder by using an aromatic amino acid decarboxylase preparation. The laying hen feed disclosed by the invention can be used for feeding laying hens in a high-temperature environment in summer, and through a unique heat stress resistant formula, the heat stress resistant capacity of the laying hens is remarkably improved, adverse effects caused by heat stress are reduced, meanwhile, the antioxidant capacity and the immune function of the laying hens are enhanced, and the growth performance and the egg laying performance of the laying hens are improved.

Aspects of the disclosure relate to aromatic amino acid ammonia lyases (ALs), phenylalanine ammonia lyases (PALs), and tyrosine ammonia lyase (TALs), including engineered enzymes, and their use in catalyzing chemical reactions.

Provided is a recombinant adeno-associated viral (rAAV) vector comprising one or two of (a) to (c): (a) a nucleotide sequence encoding aromatic L-amino acid decarboxylase (AADC), (b) a nucleotide sequence encoding glucocerebrosidase (GBA1); and (c) a nucleotide sequence encoding a neurotrophic factor (NTF), such as cerebral dopamine neurotrophic factor (CDNF) or glial cell derived neurotrophic factor (GDNF), for treating neurodegenerative disorders, particularly Parkinson's disease (PD), Multiple system atrophy (MSA), Gaucher's disease (GD), and other proteinopathies. Also provided herein are viral particles comprising the rAAV vector, a pharmaceutical composition comprising the viral particles, and uses thereof.

The application provides a tea plant gene and a method for regulating theanine accumulation in a tea plant CsSSCD1 The application belongs to the technical field of plant molecular biology. CsSSCD1 The base sequence of the gene is shown as SEQ ID No. 1. The application verifies the negative regulation of the gene by using VIGS technology and transient overexpression technology. In addition, through targeted amino acid metabolome analysis, it is found that CsSSCD1 The expression inhibition weakens the synthesis of tyrosine, the expression of the key enzyme AAAT of aromatic amino acid synthesis is inhibited, the accumulation of glutamic acid is reduced but not significant, and the accumulation of glutamine is significantly reduced, finally the degradation of theanine is blocked and the synthesis is increased. It is firstly proposed that the tyrosine degradation pathway may regulate the accumulation of theanine through metabolic pathway interaction and substrate competition mechanism, and on this basis, the key gene of the pathway is identified and functionally verified CsSSCD1 The gene is negatively correlated with theanine metabolism.

The application discloses an ammonia lyase mutant, a recombinant bacterium and application of the ammonia lyase mutant in preparation of aromatic L-amino acid, and relates to the technical field of bioengineering and enzyme catalysis. Compared with a wild-type ammonia lyase, the ammonia lyase mutant comprises any one of the following mutations: M152A and / or L149G, and the amino acid sequence of the wild-type ammonia lyase is shown as SEQ ID NO. 1. The application provides the ammonia lyase mutant with higher enzyme activity and catalytic efficiency than the wild type, which can catalyze preparation of aromatic L-amino acid with high selectivity, high stability and high conversion rate. The ammonia lyase mutant has high stereoselectivity, avoids tedious chiral separation, and can directly obtain a medical-grade high-purity product. The method for preparing aromatic L-amino acid provided by the application can reduce the cost of materials, does not need a cosolvent and other reagents, simplifies the preparation process of aromatic L-amino acid, and has good industrial application potential.

Immunoglobulin (Ig) binding proteins having one or more domains having highly hydrophobic amino acids with branched side chains (Iso, Leu, Val), or aromatic amino acids (Tyr, Phe, or Trp) corresponding to position 4 or 6 or 8 of the Ig binding protein of SEQ ID NO: 1 or functionally similar proteins are provided. The disclosed proteins have superior properties for highly efficient purification methods for antibodies (immunoglobulins), for example, the proteins have high binding capacity and high chemical stability. Also provided are affinity matrices that include the disclosed Ig binding proteins, uses of these Ig binding proteins and / or affinity matrices for affinity purification of immunoglobulins, and methods for affinity purification using the disclosed Ig binding proteins.

This invention provides the application of Lycium barbarum polysaccharide (LBP) in the preparation of drugs for the prevention or treatment of intestinal flora and metabolic disorders. Research results show that LBP can significantly improve cisplatin-induced intestinal microbiota and metabolic disorders in rats, manifested by a significant reduction in CDDP-induced serum AST and ALT levels, and improvement in the degree of liver tissue lesions. Furthermore, LBP did not exhibit toxicity in rats. LBP enhanced the diversity and richness of the gut microbiota, restored the structure of dominant symbiotic bacteria, inhibited the proliferation of potentially pathogenic bacteria, and promoted the enrichment of functional bacteria related to SCFAs generation. LBP also restored abnormal metabolism in branched-chain amino acid and aromatic amino acid metabolism, one-carbon metabolism, and lipid metabolism. This invention provides a theoretical basis for the application of natural active polysaccharides in the prevention and treatment of intestinal microbiota and metabolic disorders.

The invention belongs to the technical field of Baijiu brewing, and particularly relates to a method for directionally enhancing the flavor of high-temperature yeast for making hard liquor. In order to break through the bottlenecks of single substrate utilization and uncontrollable reaction path in the existing yeast flavor regulation and control technology, the yeast flavor regulation and control method disclosed by the invention comprises the following steps: accurately proportioning pentose, hexose, disaccharide, sulfur-containing amino acid and aromatic amino acid, pretreating, preparing a solution, treating yeast-making raw materials, accurately controlling the temperature in the whole fermentation process, turning over yeast and the like. The Maillard reaction path in the high-temperature yeast preparation process is directionally regulated and controlled. Experiments show that the content of pyrazine and terpene compounds in the high-temperature Daqu can be remarkably increased, the characteristic flavors such as wood fragrance, baking fragrance and scorch fragrance are synchronously enhanced, and a systematic technical scheme is provided for regulating and controlling the flavor quality of Baijiu.

The invention discloses a conductive graphene yarn and a preparation method and application thereof, and belongs to the technical field of conductive fibers. The conductive graphene yarn prepared by the method is obtained by blending and spinning high-dispersion graphene oxide A, polyacrylonitrile and polyvinylpyrrolidone, soaking the mixture in a hydroxylamine hydrochloride solution for the first time, soaking the mixture in high-dispersion graphene oxide B for the second time, and finally soaking the mixture in the hydroxylamine hydrochloride solution for the third time. The high-dispersion graphene oxide A is obtained by adsorbing aromatic amino acid on the surface of graphene oxide through non-covalent interaction such as pi-pi; the high-dispersion graphene oxide B is obtained by adsorbing cinnamyl aldehyde on the surface of graphene oxide through non-covalent interaction such as pi-pi; the prepared conductive graphene yarn is good in conductivity, higher in tensile strength and good in antibacterial property.

The invention provides a gambiered canton gauze imitation method based on a Maillard reaction, and belongs to the technical field of textile manufacturing. The method comprises the following steps: S1, preparing a binary glycosyl cross-linking agent mother solution; s2, preparing and applying a working solution; and S3, sequentially carrying out calendering, baking and washing post-treatment on the fabric. According to the present invention, the food additive sucralose polyaldehyde derivative reacts with the amino group, the hydroxyl group and other polar groups in the protein fabric molecule to prepare the yellowish-brown-to-black fabric, the color of the fabric is similar to the color of the gambiered canton gauze, the natural dye dioscorea cirrhosa does not need to be used, and the quality of the prepared gambiered canton gauze is stable and controllable. According to the invention, by adding the aromatic amino acid, the aromatic amino acid and the polyaldehyde derivative of the sucralose serving as a food additive are subjected to a Maillard reaction similar to that in food, so that the produced gambiered canton gauze fabric has a food fragrance and is relatively high in color fastness.

The invention discloses a method for synthesizing shikimic acid from shikimic acid dehydrogenase of a plant and microorganisms, which uses a shikimic acid synthesis method based on a shikimic acid dehydrogenase gene of the plant, and is characterized by comprising the following steps: S1, obtaining a shikimic acid dehydrogenase gene sequence from the plant; s2, performing codon optimization on the gene; s3, inserting the optimized shikimic acid dehydrogenase gene into an expression vector; s4, transferring the expression vector into escherichia coli engineering bacteria to express shikimic acid dehydrogenase; and S5, synthesizing shikimic acid through an in-vitro catalytic reaction. The screened enzyme has the advantages of few side reactions, no inhibition by high-concentration products and high activity. A recombinant strain for producing shikimic acid is obtained by utilizing the enzyme through a metabolic engineering means. The strain does not use plasmids, has strong genetic stability, does not need additional aromatic amino acids in a fermentation medium, has low production cost, high yield and yield and few metabolic byproducts, and has important industrial application value.

This application belongs to the field of biology, and specifically relates to a polypeptide and its applications. This application provides a polypeptide comprising an Alphabody and BDNF linked by a linker. The Alphabody has a structure shown in the general formula HRS1-L1-HRS2-L2-HRS3, wherein each of HRS1, HRS2, and HRS3 is independently an α-helix structure containing 2 to 4 consecutive heptapeptide repeating units, said heptapeptide repeating units being polypeptide fragments represented as "abcdefg" or "defgabc", wherein at least 50% of all a and d positions are occupied by isoleucine residues, and wherein each HRS begins with an aliphatic or aromatic amino acid residue located at the a or d position; L1 and L2 are each independently a linker fragment. The polypeptide of this application can increase the exposure of BDNF in plasma and prolong its half-life.

This invention discloses a self-assembled nanoparticle for targeted imaging and photothermal therapy of melanoma, aiming to address the problems of poor targeting, low photothermal efficiency, and poor biocompatibility in existing photothermal therapy methods for melanoma. This invention designs a self-assembled nanoparticle based on a specific peptide (SA-7), aromatic amino acid (FF), and porphyrin (TPP). The self-assembled nanoparticle can efficiently target melanoma cells and generate a strong photothermal effect under 638nm laser irradiation. Simultaneously, the nanoparticle exhibits good biocompatibility and metabolic properties, making it suitable for precise imaging and treatment of melanoma.

The invention relates to the technical field of biological extraction, and particularly discloses a method for extracting active polypeptides from freshwater fish, which comprises the following steps: taking fresh freshwater fish bones, cleaning and homogenizing; deionized water containing mandelic acid is added into the homogenate according to the solid-to-liquid ratio of (3-5): 1, the deionized water contains 0.1-0.3 wt% of a mixture of D-mandelic acid and L-mandelic acid, and the weight ratio of the D-mandelic acid to the L-mandelic acid is (52-58): (0.3-0.7); stirring the mixed solution at a constant temperature of 47-52 DEG C, adjusting the pH value to 6.7-7.0, adding a compound enzyme preparation, and carrying out enzymolysis for 4-6 hours; nitrogen is introduced every 20-30 min in the enzymolysis process, and the introduction amount is 15-25 g / h; after enzymolysis is finished, heating enzymatic hydrolysate to 92-95 DEG C, maintaining enzyme deactivation for 8-12 min, adjusting the pH to be neutral, centrifuging for 10-15 min at the temperature of 4-10 DEG C and the speed of 7000-8000 r / min, and taking supernate to obtain active polypeptide; nitrogen is periodically introduced in the enzymolysis process to directionally activate phenolic hydroxyl groups of aromatic amino acids in freshwater fish active polypeptides, so that the phenolic hydroxyl groups become efficient electron donors, and the in-vitro oxidation resistance of the polypeptides is enhanced.

The invention relates to the technical field of chiral molecule detection and analysis, in particular to an SERS (Surface Enhanced Raman Scattering) substrate based on a supramolecular plasma gold nanoparticle-mirror surface as well as a preparation method and application of the SERS substrate. The SERS substrate based on the supramolecular plasma gold nanoparticle-mirror surface sequentially comprises a substrate, an AuF thin film layer, an MPBA-beta-CD complex layer and a nanoparticle Au NPs layer from bottom to top, the MPBA-beta-CD complex layer is formed by adsorbing an MPBA-beta-CD complex on the surface of the AuF thin film layer, and a nano gap is formed between the AuF thin film layer and the Au NPs layer. According to the SERS substrate based on the supramolecular plasma gold nanoparticle-mirror surface, beta-CD captures enantiomer molecules to form a supramolecular assembly, nanoscale gap hot spots are accurately manufactured between gold nanoparticles and a gold film, and synchronous recognition and identification of aromatic amino acid enantiomers can be achieved.

The invention provides a molecular self-assembly integrin patch for regulating and controlling mechanical signals of cells as well as a preparation method and application of the molecular self-assembly integrin patch, and an extracellular matrix (ECM) bionic cell patch is constructed through a molecular self-assembly technology. According to the patch, a laminin-derived integrin binding ligand is extended and self-assembled through an N-terminal aromatic amino acid to form a fibrous nano network, and the patch has controllable nano-scale ligand distribution, network viscoelasticity and stability, and can specifically activate integrin beta1 at the top end of MSC (mesenchymal stem cell), so that a mechanical signal is conducted from a cell membrane to a cell nucleus. The process coordinates cytoskeleton reconstruction, nuclear deformation and chromatin rearrangement, and finally drives the MSC to efficiently differentiate into neuron-like cells under the condition of no exogenous gene or chemical induction, so as to express nerve markers such as TUBB3 and present functional characteristics such as spontaneous calcium transient. The cell patch can be widely applied to nerve regeneration, tissue engineering, disease model construction and intelligent biological material development.

The invention provides a special medical-purpose formula food for improving the bioavailability of aromatic amino acid and application of the special medical-purpose formula food. The invention firstly provides the formula food for the special medical purpose, the formula food for the special medical purpose comprises protein, taurine and choline, and the mass ratio of the protein to the taurine to the choline is 1: (0.0019-0.01): (0.008-0.02). The formula food for the special medical purpose can improve the bioavailability of aromatic amino acid.

The present invention relates to immunoglobulin (Ig) binding proteins comprising one or more domains having highly hydrophobic amino acids with branched side chains (Iso, Leu, Val), or aromatic amino acids (Tyr, Phe, or Trp) corresponding to position 4 or 6 or 8 of the Ig binding protein of SEQ ID NO: 1 or functionally similar proteins. The novel proteins have superior properties for highly efficient purification methods for antibodies (immunoglobulins), for example, the proteins have high binding capacity and high chemical stability. The invention further relates to affinity matrices comprising the Ig binding proteins of the invention. The invention also relates to a use of these Ig binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Ig binding proteins of the invention.

The present application relates to the field of genetic engineering, and specifically provides a PntB mutant for improving L-tryptophan yield, a genetically engineered bacterium containing the mutant, a construction method and application thereof, the present application aims at the problem of NADPH deficiency in the production process of aromatic amino acids, especially L-tryptophan, obtains a pyridine nucleotide transhydrogenase beta subunit mutant PntB(A167T) through site-directed mutagenesis, and constructs a genetically engineered bacterium containing the mutant, the genetically engineered bacterium is applied in L-tryptophan fermentation, promotes the conversion of NADH to generate NADPH, the concentration of intracellular NADPH is increased by 4.8 times, the ability of the genetically engineered strain BDP2 to produce L-tryptophan is increased by 38.8% relative to the initial strain BDX1, the yield of L-tryptophan reaches 1.02g / L, and finally the efficient synthesis of L-tryptophan is realized.

The present invention relates to non-toxic, biodegradable adhesive compositions comprising the reaction product of a linear dicarboxylic acid, a saturated triol, and an aromatic amino acid. In certain embodiments, the adhesive compositions comprise the reaction product of sebacic acid terminated-poly(ethylene glycol), glycerin, and L-DOPA. The present invention also relates to methods of using the adhesive compositions for the closure of various wounds. Further, the present invention provides surgical closure devices comprising an adhesive composition.

The invention discloses application of AtTyrDC in catalyzing aromatic amino acid to synthesize biogenic amine. A TyrDC gene (AtTyrDC) is screened and identified in an arabidopsis thaliana genome by utilizing a bioinformatics method, and the TyrDC gene is further verified to have tryptophan decarboxylase activity through in-vitro enzyme activity analysis. In addition, the invention also provides an AtTyrDC mutant, and compared with the sequence shown in SEQ ID NO.1, the mutant comprises the substitution of Q369L. The invention finds that a Q369L substituted AtTyrDC mutant obviously enhances the decarboxylation activity on a substrate aromatic amino acid. The invention lays a foundation for improving metabolic pathways to enhance the production of biogenic amines in crops, and has wide application prospects.

Provided is a method for efficiently producing various optically active aromatic amino acid derivatives by reacting a specific ester compound with an aromatic halide and zinc in the presence of a catalyst using an additive. The invention also provides amino acid derivatives that can be prepared by the method.

The present application relates to tobacco flavor type regulation composition, tobacco flavor type regulator, tobacco processing method, and belongs to the technical field of tobacco processing.The tobacco flavor type regulation composition of the present application is composed of type I amino acid (aspartic acid and / or asparagine), sweet amino acid, umami amino acid, aromatic amino acid, sulfur-containing amino acid, basic amino acid and type II amino acid (isoleucine, valine, tryptophan), and when the tobacco flavor type regulation composition of the present application is used for processing tobacco, the tobacco flavor type can be highlighted, the migration can be realized, and the quality of the tobacco can be improved.

The present application provides a nucleic acid fragment comprising a modified nucleotide sequence encoding an aromatic L-amino acid decarboxylase (AADC). The invention also provides an expression vector containing the nucleic acid fragment, a recombinant adeno-associated virus (rAAV) containing the expression vector, a pharmaceutical composition containing the nucleic acid fragment, the expression vector or the rAAV, and application of the nucleic acid fragment, the expression vector or the rAAV in treatment of neurological diseases.

The invention discloses a method for improving soybean genetic transformation efficiency based on a glyphosate screening system, which is characterized in that aromatic amino acid with proper concentration is added in an elongation stage for the first time to remarkably improve the soybean genetic transformation efficiency with glyphosate as a screening marker, and the transformation efficiency is improved by more than 8%. Meanwhile, compared with a common transformation technology system taking glufosinate-ammonium as a selection marker, the non-chimera proportion and the positive rate proportion are improved by 50% or above.