63 results about "Aromatic amino acids" patented technology

The invention belongs to the technical field of metabolic engineering and biological manufacturing, and particularly relates to an engineering bacterium for producing gallic acid (GA) and application of the engineering bacterium in production of gallic acid. According to the method, key metabolic nodes in the gallic acid production process are systematically regulated, for example, the hydroxylase activity is improved, the pyruvic acid flow direction is regulated, the aromatic amino acid precursor supply is improved, and the dynamic balance between the engineering bacterium growth and the gallic acid product synthesis is realized, so that the gallic acid yield and the carbon yield are remarkably improved. Experiments show that the gallic acid yield of the engineering bacteria prepared by the invention reaches 12.5 g / L in a shake flask experiment, and reaches 109.8 g / L in a 3 L fermentation tank; and the carbon yield is increased to 0.64 g / g glucose, which is the highest level reported in the current literature.

The invention provides a heat-stress-resistant laying hen feed and a preparation method thereof. The heat-stress-resistant laying hen feed comprises the following raw materials in parts by weight: 50-60 parts of corn, 10-20 parts of soybean meal, 5-15 parts of wheat bran, 1-5 parts of vegetable oil, 5-12 parts of fish meal, 2-5 parts of beta-alanine, 2-5 parts of activated black bean powder, 2-5 parts of quercitrin, 0.2-0.5 part of multivitamins, 4-8 parts of an electrolyte poising agent and 2-3 parts of compound amino acids. The activated black bean powder is obtained by performing enzymolysis activation on black bean powder by using an aromatic amino acid decarboxylase preparation. The laying hen feed disclosed by the invention can be used for feeding laying hens in a high-temperature environment in summer, and through a unique heat stress resistant formula, the heat stress resistant capacity of the laying hens is remarkably improved, adverse effects caused by heat stress are reduced, meanwhile, the antioxidant capacity and the immune function of the laying hens are enhanced, and the growth performance and the egg laying performance of the laying hens are improved.

Provided is a recombinant adeno-associated viral (rAAV) vector comprising one or two of (a) to (c): (a) a nucleotide sequence encoding aromatic L-amino acid decarboxylase (AADC), (b) a nucleotide sequence encoding glucocerebrosidase (GBA1); and (c) a nucleotide sequence encoding a neurotrophic factor (NTF), such as cerebral dopamine neurotrophic factor (CDNF) or glial cell derived neurotrophic factor (GDNF), for treating neurodegenerative disorders, particularly Parkinson's disease (PD), Multiple system atrophy (MSA), Gaucher's disease (GD), and other proteinopathies. Also provided herein are viral particles comprising the rAAV vector, a pharmaceutical composition comprising the viral particles, and uses thereof.

The application provides a tea plant gene and a method for regulating theanine accumulation in a tea plant CsSSCD1 The application belongs to the technical field of plant molecular biology. CsSSCD1 The base sequence of the gene is shown as SEQ ID No. 1. The application verifies the negative regulation of the gene by using VIGS technology and transient overexpression technology. In addition, through targeted amino acid metabolome analysis, it is found that CsSSCD1 The expression inhibition weakens the synthesis of tyrosine, the expression of the key enzyme AAAT of aromatic amino acid synthesis is inhibited, the accumulation of glutamic acid is reduced but not significant, and the accumulation of glutamine is significantly reduced, finally the degradation of theanine is blocked and the synthesis is increased. It is firstly proposed that the tyrosine degradation pathway may regulate the accumulation of theanine through metabolic pathway interaction and substrate competition mechanism, and on this basis, the key gene of the pathway is identified and functionally verified CsSSCD1 The gene is negatively correlated with theanine metabolism.

The application discloses an ammonia lyase mutant, a recombinant bacterium and application of the ammonia lyase mutant in preparation of aromatic L-amino acid, and relates to the technical field of bioengineering and enzyme catalysis. Compared with a wild-type ammonia lyase, the ammonia lyase mutant comprises any one of the following mutations: M152A and / or L149G, and the amino acid sequence of the wild-type ammonia lyase is shown as SEQ ID NO. 1. The application provides the ammonia lyase mutant with higher enzyme activity and catalytic efficiency than the wild type, which can catalyze preparation of aromatic L-amino acid with high selectivity, high stability and high conversion rate. The ammonia lyase mutant has high stereoselectivity, avoids tedious chiral separation, and can directly obtain a medical-grade high-purity product. The method for preparing aromatic L-amino acid provided by the application can reduce the cost of materials, does not need a cosolvent and other reagents, simplifies the preparation process of aromatic L-amino acid, and has good industrial application potential.

Immunoglobulin (Ig) binding proteins having one or more domains having highly hydrophobic amino acids with branched side chains (Iso, Leu, Val), or aromatic amino acids (Tyr, Phe, or Trp) corresponding to position 4 or 6 or 8 of the Ig binding protein of SEQ ID NO: 1 or functionally similar proteins are provided. The disclosed proteins have superior properties for highly efficient purification methods for antibodies (immunoglobulins), for example, the proteins have high binding capacity and high chemical stability. Also provided are affinity matrices that include the disclosed Ig binding proteins, uses of these Ig binding proteins and / or affinity matrices for affinity purification of immunoglobulins, and methods for affinity purification using the disclosed Ig binding proteins.

This invention provides the application of Lycium barbarum polysaccharide (LBP) in the preparation of drugs for the prevention or treatment of intestinal flora and metabolic disorders. Research results show that LBP can significantly improve cisplatin-induced intestinal microbiota and metabolic disorders in rats, manifested by a significant reduction in CDDP-induced serum AST and ALT levels, and improvement in the degree of liver tissue lesions. Furthermore, LBP did not exhibit toxicity in rats. LBP enhanced the diversity and richness of the gut microbiota, restored the structure of dominant symbiotic bacteria, inhibited the proliferation of potentially pathogenic bacteria, and promoted the enrichment of functional bacteria related to SCFAs generation. LBP also restored abnormal metabolism in branched-chain amino acid and aromatic amino acid metabolism, one-carbon metabolism, and lipid metabolism. This invention provides a theoretical basis for the application of natural active polysaccharides in the prevention and treatment of intestinal microbiota and metabolic disorders.

The invention belongs to the technical field of Baijiu brewing, and particularly relates to a method for directionally enhancing the flavor of high-temperature yeast for making hard liquor. In order to break through the bottlenecks of single substrate utilization and uncontrollable reaction path in the existing yeast flavor regulation and control technology, the yeast flavor regulation and control method disclosed by the invention comprises the following steps: accurately proportioning pentose, hexose, disaccharide, sulfur-containing amino acid and aromatic amino acid, pretreating, preparing a solution, treating yeast-making raw materials, accurately controlling the temperature in the whole fermentation process, turning over yeast and the like. The Maillard reaction path in the high-temperature yeast preparation process is directionally regulated and controlled. Experiments show that the content of pyrazine and terpene compounds in the high-temperature Daqu can be remarkably increased, the characteristic flavors such as wood fragrance, baking fragrance and scorch fragrance are synchronously enhanced, and a systematic technical scheme is provided for regulating and controlling the flavor quality of Baijiu.

This application belongs to the field of biology, and specifically relates to a polypeptide and its applications. This application provides a polypeptide comprising an Alphabody and BDNF linked by a linker. The Alphabody has a structure shown in the general formula HRS1-L1-HRS2-L2-HRS3, wherein each of HRS1, HRS2, and HRS3 is independently an α-helix structure containing 2 to 4 consecutive heptapeptide repeating units, said heptapeptide repeating units being polypeptide fragments represented as "abcdefg" or "defgabc", wherein at least 50% of all a and d positions are occupied by isoleucine residues, and wherein each HRS begins with an aliphatic or aromatic amino acid residue located at the a or d position; L1 and L2 are each independently a linker fragment. The polypeptide of this application can increase the exposure of BDNF in plasma and prolong its half-life.

The invention relates to the technical field of chiral molecule detection and analysis, in particular to an SERS (Surface Enhanced Raman Scattering) substrate based on a supramolecular plasma gold nanoparticle-mirror surface as well as a preparation method and application of the SERS substrate. The SERS substrate based on the supramolecular plasma gold nanoparticle-mirror surface sequentially comprises a substrate, an AuF thin film layer, an MPBA-beta-CD complex layer and a nanoparticle Au NPs layer from bottom to top, the MPBA-beta-CD complex layer is formed by adsorbing an MPBA-beta-CD complex on the surface of the AuF thin film layer, and a nano gap is formed between the AuF thin film layer and the Au NPs layer. According to the SERS substrate based on the supramolecular plasma gold nanoparticle-mirror surface, beta-CD captures enantiomer molecules to form a supramolecular assembly, nanoscale gap hot spots are accurately manufactured between gold nanoparticles and a gold film, and synchronous recognition and identification of aromatic amino acid enantiomers can be achieved.

The invention provides a molecular self-assembly integrin patch for regulating and controlling mechanical signals of cells as well as a preparation method and application of the molecular self-assembly integrin patch, and an extracellular matrix (ECM) bionic cell patch is constructed through a molecular self-assembly technology. According to the patch, a laminin-derived integrin binding ligand is extended and self-assembled through an N-terminal aromatic amino acid to form a fibrous nano network, and the patch has controllable nano-scale ligand distribution, network viscoelasticity and stability, and can specifically activate integrin beta1 at the top end of MSC (mesenchymal stem cell), so that a mechanical signal is conducted from a cell membrane to a cell nucleus. The process coordinates cytoskeleton reconstruction, nuclear deformation and chromatin rearrangement, and finally drives the MSC to efficiently differentiate into neuron-like cells under the condition of no exogenous gene or chemical induction, so as to express nerve markers such as TUBB3 and present functional characteristics such as spontaneous calcium transient. The cell patch can be widely applied to nerve regeneration, tissue engineering, disease model construction and intelligent biological material development.

The invention provides a special medical-purpose formula food for improving the bioavailability of aromatic amino acid and application of the special medical-purpose formula food. The invention firstly provides the formula food for the special medical purpose, the formula food for the special medical purpose comprises protein, taurine and choline, and the mass ratio of the protein to the taurine to the choline is 1: (0.0019-0.01): (0.008-0.02). The formula food for the special medical purpose can improve the bioavailability of aromatic amino acid.

The present invention relates to immunoglobulin (Ig) binding proteins comprising one or more domains having highly hydrophobic amino acids with branched side chains (Iso, Leu, Val), or aromatic amino acids (Tyr, Phe, or Trp) corresponding to position 4 or 6 or 8 of the Ig binding protein of SEQ ID NO: 1 or functionally similar proteins. The novel proteins have superior properties for highly efficient purification methods for antibodies (immunoglobulins), for example, the proteins have high binding capacity and high chemical stability. The invention further relates to affinity matrices comprising the Ig binding proteins of the invention. The invention also relates to a use of these Ig binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Ig binding proteins of the invention.

The present application relates to the field of genetic engineering, and specifically provides a PntB mutant for improving L-tryptophan yield, a genetically engineered bacterium containing the mutant, a construction method and application thereof, the present application aims at the problem of NADPH deficiency in the production process of aromatic amino acids, especially L-tryptophan, obtains a pyridine nucleotide transhydrogenase beta subunit mutant PntB(A167T) through site-directed mutagenesis, and constructs a genetically engineered bacterium containing the mutant, the genetically engineered bacterium is applied in L-tryptophan fermentation, promotes the conversion of NADH to generate NADPH, the concentration of intracellular NADPH is increased by 4.8 times, the ability of the genetically engineered strain BDP2 to produce L-tryptophan is increased by 38.8% relative to the initial strain BDX1, the yield of L-tryptophan reaches 1.02g / L, and finally the efficient synthesis of L-tryptophan is realized.

The invention discloses application of AtTyrDC in catalyzing aromatic amino acid to synthesize biogenic amine. A TyrDC gene (AtTyrDC) is screened and identified in an arabidopsis thaliana genome by utilizing a bioinformatics method, and the TyrDC gene is further verified to have tryptophan decarboxylase activity through in-vitro enzyme activity analysis. In addition, the invention also provides an AtTyrDC mutant, and compared with the sequence shown in SEQ ID NO.1, the mutant comprises the substitution of Q369L. The invention finds that a Q369L substituted AtTyrDC mutant obviously enhances the decarboxylation activity on a substrate aromatic amino acid. The invention lays a foundation for improving metabolic pathways to enhance the production of biogenic amines in crops, and has wide application prospects.

The present application relates to tobacco flavor type regulation composition, tobacco flavor type regulator, tobacco processing method, and belongs to the technical field of tobacco processing.The tobacco flavor type regulation composition of the present application is composed of type I amino acid (aspartic acid and / or asparagine), sweet amino acid, umami amino acid, aromatic amino acid, sulfur-containing amino acid, basic amino acid and type II amino acid (isoleucine, valine, tryptophan), and when the tobacco flavor type regulation composition of the present application is used for processing tobacco, the tobacco flavor type can be highlighted, the migration can be realized, and the quality of the tobacco can be improved.

The present application provides a nucleic acid fragment comprising a modified nucleotide sequence encoding an aromatic L-amino acid decarboxylase (AADC). The invention also provides an expression vector containing the nucleic acid fragment, a recombinant adeno-associated virus (rAAV) containing the expression vector, a pharmaceutical composition containing the nucleic acid fragment, the expression vector or the rAAV, and application of the nucleic acid fragment, the expression vector or the rAAV in treatment of neurological diseases.

The invention discloses a method for improving soybean genetic transformation efficiency based on a glyphosate screening system, which is characterized in that aromatic amino acid with proper concentration is added in an elongation stage for the first time to remarkably improve the soybean genetic transformation efficiency with glyphosate as a screening marker, and the transformation efficiency is improved by more than 8%. Meanwhile, compared with a common transformation technology system taking glufosinate-ammonium as a selection marker, the non-chimera proportion and the positive rate proportion are improved by 50% or above.

The invention belongs to the technical field of chemical synthesis, and relates to a method for synthesizing a chiral alpha-non-natural aromatic amino acid derivative under visible light driving. The preparation method comprises the following steps: carrying out in-situ matching on a compound 1 and a ligand under the action of a catalyst, and then continuously carrying out photocatalytic coupling reaction on the compound 1 and a compound 2 under the action of an additive to obtain a compound 3, namely the chiral alpha-non-natural aromatic amino acid derivative. Rich types of natural carboxylic acids exist in the nature, redox active ester derived from aromatic acetic acid is adopted as an aryl donor, a guarantee is provided for structural diversity synthesis of chiral alpha-non-natural aromatic amino acid, a non-natural amino acid compound library can be enriched to a great extent, and assistance is provided for development of polypeptide innovative drugs.

The application discloses a kind of high viscosity, water separable conductive glue and preparation method thereof, belong to conductive glue material technical field, the application uses mixed grinding preparation conductive glue by heteropoly acid, aromatic amino acid containing phenolic hydroxyl group and conductive component.The selection of amino acid in the application has uniqueness, it needs to meet with phenolic hydroxyl group and has certain hydrophobicity;The reaction of the application can be carried out at room temperature, without solvent, avoid the pollution caused to environment due to using a large amount of solvent;The application can effectively solve the problem that the metal salt and its oxide with strong hydrophobicity and poor solubility cannot react in aqueous solution by solid phase polymerization;The conductive glue prepared by the application can be immediately debonded after contacting water, realizes on-demand separation, can be recycled and reused after absorbing certain moisture, and the recycling mode is simple to operate, environmental protection, reduces cost.

The present invention relates to a novel zinc-binding dehydrogenase variant and a method of producing an L-aromatic amino acid using the same. The zinc-binding dehydrogenase variant is obtained by substituting one or more amino acids in the amino acid sequence constituting zinc-binding dehydrogenase to change the enzymatic activity of the zinc-binding dehydrogenase, and a recombinant microorganism comprising the variant is capable of efficiently producing an L-aromatic amino acid.

The application discloses a polypeptide with blood pressure lowering activity, which has aromatic amino acid and proline as N and C terminals. The polypeptide comprises 3-5 amino acid sequences. The polypeptide is any one of five polypeptides with amino acids FGPW, PFPRP, PFA, NPF and FPPP. The application also provides application of the polypeptide in a blood pressure lowering drug. The application designs five polypeptides with aromatic amino acid and proline as N and C terminals, simulates the interaction between a peptide segment and ACE, and the polypeptide has good blood pressure lowering effect and is non-toxic, and can be used for development of a blood pressure lowering drug.

The invention relates to the technical field of natural product enrichment, in particular to a method for enriching 4-(alpha-L-rhamnosioxy)-benzyl glucoside and related metabolites thereof in moringa oleifera bud seedlings, which comprises the following steps: 1) removing the skin of moringa oleifera seeds to obtain peeled moringa oleifera seeds; 2) treating the peeled moringa seeds with aromatic amino acid; and (3) carrying out germination culture on the treated peeled moringa oleifera seeds. According to the method provided by the invention, efficient enrichment of 4-(alpha-L-rhamnosioxy)-benzyl glucoside and related metabolites thereof in the moringa oleifera bud seedlings is realized by directionally adjusting the metabolic flux, the method meets the requirements of safety and process stability of the food industry, the cost is controllable, and the method is suitable for industrial production. And a brand new technological approach is provided for subsequent development of moringa oleifera functional food.

The invention relates to the field of gene engineering, and discloses a TolR mutant and an application of the TolR mutant in an L-phenylalanine production strain. The mutant comprises a TolR mutant obtained by mutating the 85th amino acid residue R85 of a TolR enzyme into G, A, S, T, C, V, I, L, M and P through site-specific mutagenesis. Comprising the mutant can promote the L-phenylalanine production capacity of escherichia coli, the yield is remarkably increased by 20.74%, and the L-phenylalanine gene engineering bacterium has a great application prospect.

The application discloses an antibody reagent, a polypeptide, an siRNA based on RIM1 protein expression and application of the antibody reagent, the polypeptide and the siRNA, wherein the antibody reagent is RIM1 antibody, and the concentration is 1 mg / ml; the polypeptide is based on RIM1 protein sequence, contains aromatic amino acids, and the amino acid sequence is WNQTFVYSHVHRRDF, wherein Y is tyrosine, W is tryptophan, and F is phenylalanine; the siRNA can interfere with Rim1 gene expression, the siRNA is a double strand, the sense strand sequence is UGGAAUCAAACAUUUGUCUACtt, and the antisense strand sequence is GUAGACAAAUGUUUGAUUCCAtt.The action principles of the three drugs are to realize prolonging the hot paw withdrawal latency of a limb of a neuropathic pain mouse by reducing the glutamate release of the anterior cingulate cortex of the neuropathic pain mouse, so that a theoretical inspiration is provided for the treatment of clinical neuropathic pain.

The present application belongs to the field of metabolic engineering and biological manufacturing technology, and particularly relates to an engineered bacterium for producing gallic acid and application of the engineered bacterium in producing gallic acid. The present application realizes dynamic balance between growth of the engineered bacterium and synthesis of gallic acid product by systemically regulating key metabolic nodes in the production process of gallic acid, such as improving activity of hydroxylase, regulating flow direction of pyruvic acid and improving supply of aromatic amino acid precursors, so as to significantly improve yield and carbon yield of gallic acid. It is found through experiments that the yield of gallic acid of the engineered bacterium prepared by the present application reaches 12.5 g / L in a shake flask experiment and 109.8 g / L in a 3 L fermenter; and the carbon yield is improved to 0.64 g / g glucose, which is the highest level reported in the literature at present.

A composition contains one or more aromatic amino acids and optionally vitamin B6. The composition may be an oral nutritional composition, for example a nutritional supplement, an oral nutritional supplement, a food product, a food for special medical purpose (FSMP). The composition can be in a form of a powder, a powdered stick, a capsule or a solution. The composition can be administered to an individual in need thereof orally or intravenously for preventing and / or treating sarcopenia, a loss of and / or improving skeletal muscle mass, lean muscle mass, skeletal muscle strength and / or skeletal muscle function.

The invention provides application of a CsSSCD1 gene in regulation and control of theanine accumulation of tea trees, and belongs to the technical field of plant molecular biology. The base sequence of the CsSSCD1 is as shown in SEQ ID No. 1 (sequence identifier number 1). The negative regulation effect of the gene is verified by using a VIGS technology and a transient overexpression technology. Besides, targeted amino acid metabolome analysis shows that tyrosine synthesis is weakened by CsSSCD1 expression inhibition, aromatic amino acid synthesis key enzyme AAAT expression is inhibited, glutamic acid accumulation is reduced but not obvious, glutamine accumulation is obviously reduced, and finally theanine degradation is hindered and synthesis is increased. It is proposed for the first time that a tyrosine degradation pathway may regulate and control accumulation of theanine through metabolic pathway interaction and a substrate competition mechanism, and on this basis, it is identified and functionally verified that a key gene CsSSCD1 in the pathway and theanine metabolism are in a negative correlation relationship.

Use of an aromatic L-amino acid decarboxylase as a marker in a detection product for hepatic encephalopathy. It has been confirmed that the abundance of aromatic L-amino acid decarboxylase genes encoded by gut microbiota, as well as the product of aromatic L-amino acid decarboxylase, phenylethylamine, are increased in patients with hepatic encephalopathy; and compared with blood ammonia, hepatic encephalopathy can be more accurately diagnosed by means of phenylethylamine. This suggests that the occurrence of hepatic encephalopathy and the degree of progression of the disease can be quickly confirmed by detecting the patients' feces and serum, which increases the demand for clinical detection of hepatic encephalopathy. It has been confirmed that the baseline serum phenylethylamine level can effectively predict the incidence of hepatic encephalopathy at 3 months after TIPS surgery, which provides a detection means for early warning of hepatic encephalopathy.

The present invention relates to a novel variant of DNA-binding transcriptional regulator MalT and a method of producing L-aromatic amino acids using the same. The DNA-binding transcriptional regulator MalT variant is obtained by substituting one or more amino acids in the amino acid sequence constituting DNA-binding transcriptional regulator MalT to change the activity of the protein, and a recombinant microorganism comprising the variant is capable of efficiently producing L-tryptophan, L-phenylalanine, or L-tyrosine.