The invention discloses an RNA isothermal amplification nucleic acid detection kit aiming at Escherichia coli 0157. The kit comprises a capture probe, a pair of 0157 detection primer T7 and n T7 primer, a 0157 detection probe, M-MLV reverse transcriptase enzymes, T7 RNA polymerases and other reagents. The kit provided by the invention can detect 0157 RNA in foods, has the characteristics of high specificity, high sensitivity (which can reach 103 CFU/mL), low pollution (as the amplification product RNA is susceptible to degradation in the natural environment), accurate result (as false positives can be avoided) and quick detection (as the detection can be completed within 40 minutes conventionally ), plays an important role in the quick detection of the Escherichia coli 0157, and has a wide application prospect.