The invention relates to
influenza A and B
virus nucleic acid detection primers and a kit, and belongs to the technical field of biological detection. The method aims at solving the problems that an existing
virus detection method is long in consumed time, low in sensitivity, prone to
pollution and incapable of adapting to
automation. The primer, the fluorescent probe, the
internal standard primer and the probe are designed for specific conserved regions of the
influenza A virus and the influenza B virus, and the
enzyme dosage, the primer and probe concentration and the like are optimized. The method is adaptive to automatic detection, operation transfer and
aerosol pollution are reduced, and operation is simple, convenient and time-saving; the sensitivity of
influenza A throat swab sample detection is 100%, the specificity is 99.65%, the sensitivity of influenza B detection is 100%, the specificity is 99.66%, cross reaction is avoided, and rapid and accurate detection is achieved.