61 results about "Throat swab sample" patented technology

The invention relates to a mobile throat swab robot system, and specifically relates to a mobile throat swab sampling robot, a supporting system and operation software. The sampling robot is mainly used for the throat swab sampling operation of mobile detection points, so that doctors can achieve process interference and sampling position correction. The whole system can guarantee the separation of doctors and patients during throat swab sampling, can complete operations such as contact judgment, sampling quality judgment, throat swab sampling, throat swab head cutting and transferring and hand disinfection under fully automatic or partial manual intervention and can synchronously record data including but not limited to collection strength and positions, so that the influences of highly infectious diseases represented by the novel coronavirus on medical staff can be greatly reduced, physical and mental burden of the medical staff can be relieved, and medical resources such as protective suits and gloves can be saved; and the sampling robot can be directly connected to a mobile platform, so that the rapid moving of throat swab collection place construction and the collection and sampling of throat swabs can be realized.

The invention provides an automatic throat swab sampling device which comprises a head positioning unit of a person to be detected, a navigation positioning unit and a sample collection execution unit, the to-be-tested person head positioning unit is used for realizing head positioning, opening the oral cavity of the to-be-tested person and flattening the tongue of the to-be-tested person, and providing an unobstructed space leading to the pharynx for the tail ends of the navigation positioning unit and the sample collection execution unit; the navigation positioning unit enters the oral cavity of the to-be-detected person along with the tail end of the sample collection execution unit and is used for collecting throat images and determining depth information between the depth sensor and the throat of the to-be-detected person; the sample collection execution unit comprises a three-degree-of-freedom guide rail type device and a one-degree-of-freedom tail end execution mechanism; the guide rail type device can achieve pharyngeal wiping action of a person to be tested and control over deep feeding movement and wiping action force control parts in the oral cavity of the person to be tested, and all the units are controlled by a control end in real time. The device provides a basis for full automation of the throat swab collection process.

The invention discloses a closed type throat swab sampling device. According to the device, the upper end of a handle is connected with a display screen through a connecting shaft, the lower part of the handle is connected with a long plate, the top end of the long plate is connected with a tongue depressor, and the long plate is provided with a sampling cavity and a camera; a fixed valve is arranged at the joint of the long plate and the handle; and a sealing cover is fixedly or movably sleeved on the long plate, and an elastic band is arranged at the middle hole to be tightly bonded with thelong plate. The invention has the advantages that: the device can be used to solve the problem of how to safely and correctly collect throat swab specimens by medical staff for infectious respiratorydiseases, particularly for patients with corona virus disease 2019 at present. Oral and nasal parts of the patient are properly covered in a closed manner, thus avoiding an extremely high risk of infection since the medical staff directly face respiratory tract pathogenic bacteria when collecting throat swab specimens of patients; and a real-time image in the oral cavity is displayed in vitro byleading-in the camera with a light source to the oral cavity, and the medical staff can correctly and quickly acquire an ideal throat swab specimen under the guidance of the real-time image.

The invention provides a throat swab sampling system and a sampling method. The throat swab sampling system and the sampling method have the advantages that the oral cavity of a patient to be sampled is positioned through a camera and a laser range finder in a sampling device, so that the sampling accuracy of a throat swab is further improved, the accurate positioning of the throat swab sampling is guaranteed, and the parallax influence is reduced; a pressure sensor is arranged in the sampling device, when the throat swab is in contact with a sampling part, a pressure value fed back by the pressure sensor changes, and the device stops moving; a buffer mechanism is arranged in a sampling head device, so that the contact is relatively soft, and the sampled patient does not have obvious pain; and under the isolation condition, medical staff remotely controls the throat swab sampling system and moves the throat swab sampling system to the front of the patient to sample the oral cavity of the patient, so that the infection caused by direct contact between the medical staff and the patient in the sampling process can be avoided, the patient can be safely subjected to deep examination, the sampling safety of the throat swab is improved, and the safety of the medical staff is guaranteed.

The invention discloses a direct real-time quantitative PCR method of a throat swab sample or a nasopharyngeal swab sample. The method comprises the following steps: (1) lysate and a PCR reaction solution are prepared; (2) the throat swab sample or the nasopharyngeal swab sample are added into a PCR amplification tube containing the lysate in the step (1), magnetic beads are added into the PCR amplification tube, and vibration, uniform shaking and mixing are carried out; (3) the PCR amplification tube in the step (2) is treated at a high temperature 80-100 DEG C for 10 minutes, cooling is carried out to a room temperature, and a reaction is carried out at 18-28 DEG C for 5-10 minutes; (4) the PCR amplification tube is instantly centrifuged and placed into a magnetic rack, after the magnetic beads are absorbed to one side of a magnetic rack, supernatant is sucked out; (5) the PCR amplification tube is added into a prepared PCR reaction solution, and the real time quantitative PCR reaction is carried out; the lysate comprises the following components 0-2M NaCl, 0-0.3% Triton-X and 0-0.2M KCl, the magnetic beads are hydroxyl magnetic beads sold on the market for extracting nucleic acid, the throat swab sample detection is rapid, simple, high-efficient and practical, nucleic acid extraction process and centrifuge and other equipment are not needed, operation processes are simplified, time and cost are saved, a large amount of samples can be processed, and during operation and detection, pollution is prevented.

The invention relates to the technical field of virus nucleic acid detection methods, and particularly relates to an RT-LAMP nucleic acid detection primer group of SARS-CoV-2 and a closed SARS-CoV-2 isothermal amplification nucleic acid detection kit. According to the kit provided by the invention, nucleic acid extraction is not needed for virus RNA of a throat swab sample, and the sample can be directly amplified. The kit is a visual instant screening kit for the SARS-CoV-2 by a one-step method. The kit has good specificity, can eliminate non-specific interference of other coronavirus strains, is quick and efficient, and can complete detection within one hour. Large expensive and precise instruments and equipment are not needed, only a common metal bath or water bath kettle is needed, detection cost is low, toxic reagents are not involved and closed operation is performed in the whole process, secondary pollution is avoided, and safety of operators and an environment is ensured.

The invention discloses a novel coronavirus 2019-nCoV nucleic acid kit and a virus nucleic acid acquisition method. The method comprises the following steps: according to novel coronavirus 2019-nCoV ORF1ab and an N gene as amplification target areas, designing specific primers and fluorescent probes for detecting novel coronavirus 2019-nCoV nucleic acid in samples; and performing Digital PCR (polymerase chain reaction) absolute quantification on 2019-nCoV nucleic acid RNA (ribonucleic acid) by using a one-step method by using a prepared Super premix reaction liquid A together with Super premixreaction liquids B and C, and performing Digital PCR detection on RNA of a diluted positive virus sample. The kit and the method have the beneficial effects that by using the novel virus nucleic acidacquisition method, and together with a Digital PCR probe method, the nucleic acid positive detection rate of infection of the novel coronavirus 2019-nCoV can be increased, the process is ensured tobe specified and standardized, the influence of artificial non-standard operation upon detection results can be reduced, result reliability can be ensured, and meanwhile, false negative results causedby detection of sputum and throat swab sampling together with real-time fluorescent PCR methods can be avoided.

The invention provides a fluorescent RT-RAA (Reverse Transcription Recombinase Aid Amplification) primer for detecting MERS-CoV (Middle East Respiratory Syndrome Coronavirus), a fluorescent probe andapplication of the fluorescent RT-RAA primer and the fluorescent probe in detecting the MERS-CoV. The fluorescent RT-RAA primer and the fluorescent probe, disclosed by the invention, are capable of quickly and qualitatively detecting the MERS-CoV in a throat swab sample, detection can be carried out under the normal temperature of 39 DEG C, a diagnosis result can be obtained within 20 minutes, andthe detection time is greatly shortened; the fluorescent RT-RAA primer and the fluorescent probe are stronger in specificity and higher in sensitivity, quick diagnosis and whole-process monitoring onan epidemic situation can be completely met, and time is bought for early diagnosing and early treating the epidemic situation, reducing a case fatality rate and controlling the epidemic situation.

The invention discloses a flexible throat swab sampling device. The flexible throat swab sampling device comprises a flexible throat swab body and a feeding device, wherein an airway is arranged in the flexible throat swab body, and the flexible throat swab body is arranged to be sleeved with a sampling part; the feeding device is connected with the flexible throat swab body; the airway is arranged for gas to enter; the flexible throat swab body is arranged to deform under the action of the gas entering the airway, so that the sampling part located in the mouth of a user scrapes the inner wallof the mouth of the user; and the feeding device is arranged to drive the flexible throat swab body to move. The flexible throat swab body of the flexible throat swab sampling device is made of a soft material, and can be bent as required; on the one hand, through friendly interaction with human bodies, symptoms such as nausea and cough caused by discomfort of patients are reduced, and viruses are prevented from spit and infection.

The invention relates to a method for detecting component information of a throat swab sample by adopting surface enhanced micro-Raman spectroscopy. The method comprises preparation of a surface enhancement reagent, treatment of the throat swab sample, measurement of a surface enhanced Raman spectrum of the sample and processing and analysis of Raman spectrum data. According to the technical scheme, the method comprises the following steps: preparing the enhancement reagent for enhancing the Raman spectrum of the sample; smearing the throat swab sample on a smooth and clean pure aluminum sheet, dripping the surface enhancement reagent, and naturally air-drying the pure aluminum sheet at room temperature to prepare a throat swab-surface enhanced Raman test sheet; performing micro-Raman spectroscopic test on the throat swab-surface enhanced Raman test sheet to acquire the surface enhanced micro-Raman spectrum of the throat swab sample, and analyzing biochemical component information and features of the throat swab sample by virtue of spectral features. The method has the advantages of short detection time, low cost, convenience and rapidness in process and the like, and rich biochemical component information of the throat swab sample can be obtained, so that a rapid and effective analysis method can be provided for biochemical features and changes of intracavity mucous tissues.

The invention relates to the technical field of molecular biology, and discloses primers, probe, method and kit for detecting mycoplasma pneumoniae nucleic acid and drug-resistant mutation through a real-time fluorescent PCR. By means of a Taqman-MGB probe real-time fluorescent PCR method, the primers and the fluorescent marking probe are designed for a mycoplasma pneumoniae specificity P1 gene, 23S rRNA gene drug resistance mutation genes (2063A/G and 2064A/G) and interior label (int) nucleic acids, the detection result is judged through the combination of an amplification curve and a CT value through PCR reaction detection fluorescent signals, and a target gene is detected through the change of the fluorescent signals. The primers, probe, method and kit have the advantages of being high in accuracy, specificity and sensitivity, and mycoplasma pneumoniae and drug resistance mutation sites in a throat swab sample can be rapidly and accurately detected.

The invention discloses a handheld movable throat swab sampling gun. The handheld movable throat swab sampling gun comprises a sampling gun body, a swab clamp, a swab rod and a display screen, wherein the sampling gun body comprises a gun barrel and a handle, the swab clamp is inserted into the upper portion of the gun barrel, the swab rod is pushed out of the gun barrel from the swab clamp through an ejector pin in the gun barrel, a rotary motor installed at the end of the gun barrel drives the swab rod to rotate, a camera is installed at the top of the gun barrel and connected with the display screen to display pictures in an oral cavity, a trigger is arranged at the joint of the handle and the gun barrel, and a battery and a central processing unit are installed in the handle. According to the invention, the battery supplies power to the central processing unit, the rotary motor and the trigger are connected with the central processing unit, and the rotation of the swab rod is controlled through the central processing unit. The handheld movable throat swab sampling gun of the invention solves the problems that an existing throat swab is low in collection efficiency, not visual and poor in protection to medical staff.

The invention discloses an automatic new coronavirus nucleic acid sampling detection test tube collecting device in the field of test tube collection, which comprises a test tube placing tool arrangedon a workbench. The test tube placing tool is respectively provided with an undetected area, an under-detection area and an already-detected area. A plurality of new sampling test tubes are placed inthe undetected area. At least one under-detection sampling test tube is placed in the detection area, a plurality of already-detected sampling test tubes for collecting throat swab specimens are placed in the already-detected area, and a sampling test tube moving device for moving and switching the positions of the sampling test tubes among the undetected area, the under-detection area and the already-detected area is arranged on the workbench corresponding to the test tube placing tool. The sampling test tube moving device comprises a three-axis robot arranged on the workbench, and a cap screwing grabbing mechanism capable of grabbing a sampling test tube and opening and closing a test tube cap is arranged on the three-axis robot. Multiple throat swab samples can be collected and storedin the sampling test tube, the infection risk is reduced, and the detection sample collection efficiency is improved.

The invention discloses a pharyngeal swab collection vehicle and relates to the technical field of pharyngeal swab collection. The pharyngeal swab collection vehicle comprises a collection vehicle, one end of the collection vehicle is provided with a collection bin, a top part of the collection bin is provided with a negative pressure system, one side of an inside of the collection bin is providedwith a disinfection component, one end of the inside of the collection bin is provided with a robotic arm assembly, a top part of the robotic arm assembly is provided with a grasping component, and abottom part of the collection bin is provided with a hydraulic system; and the negative pressure system comprises a virus filter pipe and a depressurization exhaust pipe, the virus filter pipe is connected to one end of the depressurization exhaust pipe, and the virus filter pipe communicates with the depressurization exhaust pipe. The pharyngeal swab collection vehicle can avoid direct contact between patients and medical staff, and reduce risks of infection of the medical staff; moreover, a special pharyngeal swab sample collection area chamber can be provided to prevent air inside the collection area from being unsterilizedly spread out at will; and at the same time, the device can be moved and easily moved to the area where a large number of pharyngeal swab samples need to be collected.

The invention provides novel high-sensitivity respiratory virus nucleic acid NASBA (nucleic acid sequence based amplification) primers. A respiratory virus conservative area is selected, and a promoter sequence capable of being recognized by T7 RNA (ribose nucleic acid) polymerase is formed at the 5' terminal of one of the synthesized primers. The invention further provides a novel high-sensitivity respiratory virus nucleic acid NASBA detection method. T7 RNA polymerase promoter sequences are added to the 5' terminals of the two primers complementary to two ends of a respiratory virus RNA sequence, so that when the primers enter a circulation phase, RNA sequences [RNA(+)] consistent with a template sequence can be synthesized, RNA sequences [RNA(-)] complementary to a template RNA sequence can be synthesized, and the detection sensitivity of viral nucleic acid is greatly improved; a throat swab sample of a patient can be directly used for amplification, a reverse transcription process is not required, and the operation time is saved.

The invention belongs to the technical field of sampling methods, and particularly relates to a throat swab sampling tube for preventing new coronavirus from diffusing through aerosol and a use method thereof.The throat swab sampling tube for preventing new coronavirus from diffusing through aerosol is provided with an upper bottle body and a lower bottle body, and a virus aerosol sealing film is arranged in the upper bottle body; the virus aerosol sealing film is provided with a sampling puncture area and a virus aerosol filtering film arranged around the sampling puncture area, the sampling puncture area is convenient for the pipettor to puncture and suck throat swab preserving fluid for nucleic acid detection, and the virus aerosol filtering film is made of a breathable material, is communicated with the outside and can filter virus aerosol and prevent the virus aerosol from diffusing to the outside. A throat swab filter screen is arranged in the lower bottle body, the throat swab is arranged in the lower bottle body, the throat swab and falling fibers of the throat swab can be filtered through the throat swab filter screen, the throat swab and the falling fibers of the throat swab are prevented from entering a throat swab preserving fluid collecting cavity in the lower portion, a pipettor can conveniently suck a sample, and virus aerosol generated by repeated sample suction is avoided.

The invention provides a primer group and a probe group capable of simultaneously detecting various influenza viruses. Sequences of the primer group are shown as SEQ ID NO:1-8, and sequences of the probe group are shown as SEQ ID NO:9-12; and the influenza viruses include influenza A virus, influenza B virus, seasonal influenza virus H1 sub-type and H3 sub-type. On the basis, a kit, which is capable of simultaneously detecting the various influenza viruses, is provided. According to the prime group, the probe group and the kit, direct amplification and multiplex PCR are combined; direct RT-PCR can be conducted on suspected influenza cases or epidemiological investigation throat swab samples; and in comparison with a conventional method, a nucleic acid extraction step is avoided, so that it is conducive to implementation of automatic integrated detection and to improvement of efficiency. The prime group, the probe group and the kit have obvious advantages in aspect of being applied to rapid discrimination and detection of people infected by the influenza viruses, and the invention is simple and convenient to operate, short in detection time and relatively low in detection cost. The prime group, the probe group and the kit are applicable to rapid detection at a site of epidemic situation; and the prime group, the probe group and the kit have a broad and practical application value.

The invention discloses a throat swab sampling safety protection operation apparatus, and relates to the technical field of medical protection equipment. According to the throat swab sampling safety protection operation apparatus, the risk that medical staff and normal collected people are infected during current throat swab collection is solved. The throat swab sampling safety protection operation apparatus is provided with a negative pressure system, a collection chamber, an isolation chamber, a waste collection bag and a throat swab culture tube collection bag, wherein a transparent isolation plate is sequentially provided with a waste collection bag fixing sleeve pipe, two sealing arm gloves and a throat swab culture tube collection bag fixing sleeve pipe. The throat swab sampling safety protection operation apparatus is simple in structure and easy to operate, and the hidden danger that medical staff and other sampled personnel are infected is completely eliminated.

The invention relates to a construction site emergency management system. The construction site emergency management system comprises an intelligent robot, an intelligent detection device and a dronedisinfection unit, the intelligent robot is connected with a grade-A tertiary hospital health system, the intelligent robot, the drone disinfection unit and a monitoring group are connected with an administrator computer data terminal and a cloud database, the intelligent robot comprises a robot main body, mechanical arms arranged on the two sides of the robot body, a throat swab sample storage box arranged in the center, a medical high-definition camera, a voice guiding device, a medical material storage box arranged at the lower portion, a daily material storage box, a moving device arrangedat the bottommost portion and a telescopic device arranged at the bottom, wherein the medical high-definition camera and the voice guiding device are arranged at the top of the robot, and the intelligent detection device comprises a body temperature detector, a heart rate detector, a virus nucleic acid detector and an alarm device. The construction site emergency management system is novel in design, high in practicability, high in safety degree, good in timeliness, high in social benefit, good in development prospect and high in intelligent degree.

The invention provides a throat swab. The throat swab comprises a sampling assembly and a protection umbrella, wherein the sampling assembly comprises a swab head and a swab rod; the swab head is arranged at the first end of the swab rod; and the swab rod is used as an umbrella stem and can be opened towards the direction opposite to the swab head. Through the adoption of the throat swab disclosedby the invention, when acquisition of a throat swab sample is performed on a patient, the protection umbrella is arranged at a position close to the mouth and nose end of the patient, so that droplets and aerosol which are splashed by the patient can be effectively isolated, and in the acquisition process, the exposure risk of medical care personnel and pollution to the periphery environment of the patient are effectively reduced; and the throat swab is simple in structure and good in protection effect.

According to the throat swab sampling device and method, a throat swab sample collecting mechanism is fixed to a walking mechanism and used for collecting and storing sampling sample bottles, a mechanical arm is adopted in the throat swab sampling mechanism, a clamping part used for clamping the sampling sample bottles is arranged at the end of the mechanical arm, and a disinfection mechanism is fixed to the upper end of the walking mechanism and used for disinfecting the sampling sample bottles. The end part of the mechanical arm can rotationally extend into the disinfection mechanism, the disinfection mechanism is used for disinfecting a clamping part at the end part of the mechanical arm, and the movable walking mechanism is used as a sampling sample collecting device for automatically storing and clamping, so that close-range contact between a tested person and a medical worker is avoided, and the safety of the medical worker is improved; meanwhile, a machine is adopted for collection, long-time detection can be achieved, the detection efficiency is greatly improved, the working intensity of medical staff is reduced, a groove is formed in the upper end of the walking mechanism, and the accuracy of unused throat swabs in a sampling sample bottle and the accuracy of sampled samples are ensured.

The invention relates to an H1N1 influenza virus detection method and a kit thereof. According to the invention, a simple and rapid chemical coupling method is utilized to realize efficient capture ofan inactivated complete H1N1 influenza virus; and a DNA aptamer capable of specifically recognizing the inactivated A-type H1N1 virus is used as a recognition probe to achieve specific recognition ofthe virus, and finally quantitative detection of the inactivated complete H1N1 influenza virus is achieved through real-time quantitative PCR. A detection limit is 1ng/L, and the kit has no responseto other viruses and has good specificity. Colorimetric detection of the H1N1 virus can be achieved by combining with nanogold, and the detection limit is 1 ng/L. According to the method, the completeinactivated virus serves as a target, the low-cost DNA aptamer serves as a recognition molecule, and advantages of good safety and low cost are possessed. Moreover, the method and the kit are high insensitivity and good in specificity, are successfully used for detecting the H1N1 in a throat swab sample, and have a very good clinical application value.

The invention relates to a virus detection method, in particular to a throat swab virus detection method with isolation, inactivation and protection functions. The throat swab virus detection method comprises multiple groups of acquisition chambers and a group of collection chambers, a servo motor is adopted to control a low-speed positioning belt conveying structure to realize conveying of throatswab before collection and throat swab samples, and in combination with glove box operation, a positive pressure air blowing system and an ultraviolet inactivation structure, complete isolation of collection workers from patients is achieved, cross infection between the patients is avoided, and further spreading of novel coronavirus diseases is basically eradicated. Complete isolation between acquisition persons and to-be-detected persons is realized, and omnibearing safety protection is achieved; and the acquisition persons have no psychological burden while effective protection is achieved,more efficient and accurate acquisition by the acquisition persons for a longer time is ensured, the accuracy and efficiency are high in combination with annular light source structures, and the throat swab virus detection method is particularly suitable for throat swab acquisition protection detection of extremely dangerous infectious disease sources.

The invention provides a metagenomics-based respiratory tract throat swab sample library building method and a pathogen detection method, and relates to the technical field of microbiological detection. The library building method comprises the following steps: (a) pretreatment of a to-be-detected sample solution: adding lysozyme for digestion; (b) extraction of total nucleic acid of the sample: not adding carrier RNA in an extraction process; (c) cDNA synthesis: carrying out reverse transcription by adopting a random primer; (d) purifying a product synthesized in step (c) by using magnetic beads; and (e) construction of a sample DNA library. Based on a DNA-RNA co-sequencing principle, the invention provides the metagenomics-based method suitable for throat swab sample library building and detection and pathogen detection of a throat swab sample metagenome can be realized through a single reaction.

The invention discloses a test tube comprehensive flow device for a throat swab collection vehicle and a test tube, and relates to the technical field of throat swab collection test tube flow replacement. The test tube comprehensive flow device comprises a test tube flow assembly; the test tube flow assembly comprises a test tube moving cavity, a pressing cap box, a disinfection bin, a sample collection test tube and a notch, wherein the disinfection bin is arranged at one end of the test tube moving cavity; the pressing cap box is arranged on one side of the test tube moving cavity and communicates with the test tube moving cavity; the notch is formed in the position, close to the middle, of the top of the test tube moving cavity; and the sample collection test tube is placed in the testtube moving cavity. The test tube can be automatically replaced in a flowing manner, so that the possibility of direct contact of a medical staff is reduced; moreover, a throat swab sample can be automatically sealed without manual operation, so that infection is avoided; and meanwhile, disinfection is conducted on a test tube rack bearing the test tube and the exterior of the test tube in the device, so that virus in a throat swab sealed collection area can be effectively prevented from flowing out, and the infection risk is reduced.

The invention provides a cap-unscrewing and shearing system for a throat swab sampling system. The cap-unscrewing and shearing system comprises a clamping assembly for clamping a test tube, a cap unscrewing assembly for unscrewing a cap of the test tube and a shearing assembly for shearing the head of a throat swab sample in the test tube. The cap-unscrewing and shearing system is characterized in that the cap unscrewing assembly comprises clamping jaws used for clamping the cap; a spring is arranged in a containing groove of each clamping jaw, one end of each spring is fixed to the inner wall of the corresponding clamping jaw, and the other end of each spring is fixed to the upper portion of a clamping block; the clamping blocks are connected with the clamping jaws through pin shafts, so that the clamping blocks can rotate in the axis direction of the pin shafts; and an angle sensor is arranged in each clamping block, and the angle sensors correct the clamping distance between the clamping jaws based on the angle changes of the clamping blocks. The clamping distance between the clamping jaws is controlled and corrected through a control unit until the angles of the clamping blocks do not deflect, threads on the outer side walls of the clamping blocks are adjusted to abut against a thread on the outer wall of the cap, and therefore the effectiveness of unscrewing the cap is effectively improved.

The invention provides a fluorescent RT-RAA primer for detecting an A(H1N1) virus by using a reverse transcription recombinase mediated nucleic acid amplification technique, a fluorescent probe, and an application of the fluorescent RT-RAA primer and the fluorescent probe in detecting the A(H1N1) influenza virus. By adopting the fluorescent RT-RAA primer and the fluorescent probe, the A(H1N1) influenza virus in a throat swab sample can be rapidly and quantitatively detected, detection can be implemented at normal temperature of 39 DEG C, diagnosis results can be obtained within 20 minutes, andthe detection time is greatly shortened; the fluorescent RT-RAA primer and the fluorescent probe are respectively good in specificity and relatively high in sensitivity, rapid diagnosis and whole monitoring on epidemic situations can be met, and time for diagnosing and treating epidemic situations at an early stage, reducing the case fatality rate and controlling the epidemic situations is achieved.