The application belongs to the technical field of biological detection, and particularly relates to an isothermal amplification detection
reagent and kit for detecting varicella-zoster
virus (VZV). The application designs a specific primer pair with a target of a conservative region of an ORF62 of a VZV
genome, performs RPA reaction, combines a modified detection probe, and uses lateral flow
chromatography test paper to realize a detection sensitivity of not higher than 1 copy / µL for a crude extract of a
vesicle fluid or
throat swab sample, realize rapid and high-sensitivity detection, and has good popularization and application value.