199 results about "Predation" patented technology

The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.

An organic tea cultivating method is characterized by comprising the steps of base selection, base construction, tea tree plantation, fertilizer application, plant disease and insect pest ecological prevention and tea tree pruning. By means of the organic tea cultivating method, pollution-free organic cultivation is achieved, the national environment-friendly requirement is met, and produced tea leaves can meet the strictest detection of any developed country. In the organic tea cultivating method, pollution-free pest control is achieved creatively through the methods such as lamp light trap, ladybird prevention and bird predation for the first time, and the disease resisting performance of tea trees is improved by applying organic fertilizer and through reasonable layout. The pollution-free tea leaves produced through the method are good in taste and high in yield, the high-yield duration can reach 50 years or more and is far longer than the traditional 10 years of duration, and the economic benefit is quite obvious.

The invention discloses a method for cloud manufacturing resource optimization configuration based on an improved whale algorithm, and the method comprises the steps: building a problem model, and defining a fitness function; setting improved whale algorithm parameters, and generating an initial population; Calculating fitness values of all individuals in the population, obtaining a current optimal resource allocation scheme and converting the current optimal resource allocation scheme into whale individual position vectors; Introducing a parameter p, and judging whether p is less than or equal to 0.5; If not, performing spiral motion iteration updating to complete population updating; If yes, whether the value A (1) of the coefficient vector of the improved whale algorithm is met or not is judged; If yes, performing shrinkage encircling iteration updating; If not, performing random search predation iteration updating; Obtaining a current optimal resource configuration scheme; Adding 1to the number of iterations, and judging whether the current number of iterations is smaller than the maximum number of iterations; If yes, repeating the operation; And if not, outputting the currentoptimal resource configuration scheme. The whale algorithm is improved, so that the algorithm convergence speed is higher, the optimal solution is easier to achieve, and a new method is provided forsolving the problem of resource allocation.

The invention provides a coordination control method for a multi-unmanned aerial vehicle team based on predation escape pigeon optimization. The method comprises seven steps: firstly, initializing algorithm parameters and predation escape parameters; secondly, initializing initial positions and speeds of the pigeons and calculating respective fitness values; thirdly, introducing a map compass operator and updating the speeds and the positions of the pigeons according to a formula; fourthly, introducing a predation escape process and updating the positions of the pigeons according to the formula; fifthly, stopping the map compass operator and the predation escape process and executing the next step if the iteration frequency is greater than the maximum iteration frequency T1 of the map compass operator; repeating the previous step if not; sixthly, introducing a landmark operator and ranking all the pigeons according to the fitness values of residual pigeons (wherein half of the pigeons with low fitness value follow the other half of the pigeons with high fitness value), and updating the positions according to the formula; seventhly, stopping the landmark operator and outputting the final result if the current iteration frequency is greater than the maximum iteration frequency T2 of the landmark operator; and repeating the previous step if not.

The invention relates to a safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis. The safety evaluation method includes the five parts including Tier-1 toxicity testing, three-level nutrition testing, protein transferring rules, predation function reaction and population density and population dynamic comparison. The potential toxicity of Bt protein expressed by Bt transgene insect-resistant rice on the cyrtorhinus lividipennis are respectively evaluated by the five parts; influences of the Bt transgene insect-resistant rice on the cyrtorhinus lividipennis on prey media are evaluated; paths and degrees for the cyrtorhinus lividipennis to be exposed in Bt protein are evaluated; influences of the Bt transgene insect-resistant rice on the cyrtorhinus lividipennis population are evaluated. Finally generated ecological effects of transgenic crop planting on non-target organisms are comprehensively evaluated according to data in the five parts. The safety evaluation method is systematic and scientific, and is of great significance on perfecting influence studying of transgenic crops on the non-target organisms and determining the ecological safety of transgenic crop planting.

The invention discloses an ecosystem model and biomanipulation technology based eutrophic water body remediation method. Firstly, an ECOPATH model is used to fit the energy flowing mode of the currentecosystem, when the annual production of phytoplankton or aquatic macrophyte in a water body is too high, the minimum primary production requiring for maintaining the operation of the system is takenas the baseline according to the output result of the model, the consumption of the extra primary production in the system is increased through the predation action of fish by using a method of quantitatively adding a fish consumer, and meanwhile, the top-down effect is produced to control the biomass and the community structure of a primary producer. After finishing remediation, the ECOPATH model is used to access the fish catch, the commercial fish species added in the same year are harvested according to the assessment result, so that the balance of energy of the ecosystem is maintained, the fishing catch directly takes away a large quantity of nutritive salts in the water body, and certain economic value is produced. The novel thinking for reference and the technical support are provided for the remediation method taking the scientific experiment as the method from the perspectives of ecology, quantity and energetics, and the method has a very important application value for the ecological remediation of the damaged water body.

Methods for increasing the resistance to rumen inactivation of a cultured Gram positive bacteria strain useful for gastrointestinal delivery of bioactive compounds to ruminants, which includes the steps of growing a culture of the bacteria strain through at least one passage in a growth medium containing an amount of lysozyme effective to induce the growth of bacterial cell walls resistant to protozoal predation; and recovering the bacteria strain from the lysozyme-containing medium. Rumen-bypass feed supplements produced by the inventive method are also disclosed, as well as methods for supplementing the diet of a ruminant with the rumen bypass feed supplements and an in vitro method for evaluating the resistance of Gram positive bacteria strains to rumen inactivation in vivo.

Embodiments of the present invention relate to insecticidal Bacillus thuringiensis Cry1 and Cry2 polypeptides. Methods for using the polypeptides and nucleic acids of embodiments of the invention to synergistically enhance resistance of plants to insect predation are encompassed in embodiments of the present invention.

A method for controlling the pests in agricultural field and fruit garden by use of entomogenous fungi carried by predatory mites features that the proportional mixture of the predatory mites and the pathogenic microbes of pests or harmful mites (including entomogenous fungi, bacteria and virus) is packed in bags containing water and air, and than applied in agricultural field and fruit garden to eliminate pests.

The invention discloses myxococcus stipitatus preying on plant pathogenic bacteria and application thereof in biological control of bacterial diseases. Slime bacteria BS of antagonistic plant pathogenic bacteria is provided, and the deposit number is CCTCCNO. M2017496. The myxococcus stipitatus BS inhibits the growth of various plant pathogenic bacteria such as cabbage soft rot pathogen, calla soft rot pathogen, tomato bacterial wilt pathogen, potato black smut and pear fire blight by predation, and shows slime bacteria BS broad spectrum resistance to pathogenic bacteria. Pot experiments showthat the slime bacteria BS can effectively inhibit the infection of Pectobacterium carotovorum subsp. carotovorum on potatoes and calla, reduce the incidence of soft rot, and can be used for the biological control of the plant diseases caused by plant pathogenic bacteria.

The invention provides a wild-training method for improving the feeding ability of released Acipenser dabryanus. The wild-training method comprises the following steps that fish bait is produced; feeding domestication is conducted, wherein feeding domestication starts when are hatched, feeding domestication is conducted on the Acipenser dabranus which are fed with artificial feed for 2-4 months and feeding domestication is conducted on the Acipenser dabranus which are fed with the artificial feed for over 5 months; Through the wild-training method for improving the feeding ability of the released Acipenser dabrayanus, after training is over, both the feeding capability and the predation capability of the Acipenser dabryanus with different specificications are obviously enhanced and improved. After analog wild-release is conducted, the trained Acipenser dabryanus is released to the wild or an experimental pond, the capability of preying on wild life live baits is obviously improved, and the growth rate and the survival rate are obviously higher than the released Acipenser dabryanus which is not trained.

The invention relates to a high-throughput multi-LDR parting kit for detecting farmland ecological environment, comprising mitochondria COI gene universal primers of 16 kinds of species in the farmland, specific probe sequences of the mitochondria COI gene universal primers of the 16 kinds of species in the farmland, and a reagent for PCR (polymerase chain reaction) proliferation and LDR (light dependent resistor) detection programs, wherein the mitochondria COI gene universal primers have the upstream of 5'-GGTCAACAAATCATAAAGATATTGG-3' and the downstream of 5'-taaacttcagggtgaccaaaaaatca-3'. In the invention, the kit effectively proliferates all target species sequences for the COI gene universal primers, and ensures that each target sequence and a low-copy template can be detected; the specific probe of each target species further ensures the specificity so as to totally distinguish each target sequence to realize multi-parting fixed quantity, thus the change of a farmland predation structure is known and the farmland ecological environment is maintained.

The invention provides a method for luring Chrysopa sinica Tjeder to restrict Toxoptera aurantii Boyer by synomone from tea shoots and aphid sex pheromones. According to the invention, the synomone from tea shoots and the aphid sex pheromones are taken as basic components to prepare a Chrysopa sinica Tjeder trapping agent, which is used to lure the Chrysopa sinica Tjeder to trap an important injurious insect, Toxoptera aurantii Boyer, of the buds and leaves of tea trees, and used chemical pesticides can be reduced and even avoided so as to alleviate the pollution. The Chrysopa sinica Tjeder is the dominant species natural enemy of the Toxoptera aurantii Boyer, and by using the trapping agent to lure and guide the Chrysopa sinica Tjeder to restrict the Toxoptera aurantii Boyer, the predation efficiency can be improved, and the potency can be protected and utilized. The method provided by the invention protects the biodiversity, uses the pheromone substances to lure the important natural enemy, Chrysopa sinica Tjeder, to trap the Toxoptera aurantii Boyer effectively in a tea garden, protects and utilizes the natural enemy resources, and is simple and convenient to implement and low in cost.

The invention discloses application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria. The Myxococcus sp.e-3-1, Polyangium sp.8#-3, Cystobacter sp.XJ9-1 involved in the invention on one hand can prey and lyse various plant pathogenic bacteria, and on the other hand can produce active natural products inhibiting Erwinia persicina, thus having important application value. The Myxococcus sp.e-3-1, Polyangium sp.8#-3 and Cystobacter sp.XJ9-1 can be applied in biological control, drug development and other aspects.

The invention discloses an ecological Australia murray cod ichthyophthiriusmultifiliis preventing technology. When the ichthyophthiriusmultifiliis occurs on Australia murray cod, sea water is added into a water body where the Australia murray cod is located, detecting is conducted timely through a salinity meter, the salinity is controlled to be 8 thousandths to 10 thousandths, soaking is conducted for 48h, and the ichthyophthiriusmultifiliis is killed. After conducting some deep and basic research on the ultrastructure, the infection mechanism, pathogenesis and immunoreaction and the like of the ichthyophthiriusmultifiliis, the fact that a life cycle of the ichthyophthiriusmultifiliis is divided into stages of trophozoite, cyst and predation is found out. An ichthyophthiriusmultifiliis body is weak during the predation stage and can be restrained effectively by changing the environment, bad effects of pesticide on fish can be avoided, and meanwhile the ichthyophthiriusmultifiliis can be killed thoroughly and effectively.

The invention provides a wild domestication system and domestication method for proliferation and release marine fish fries, and belongs to the technical field of marine biological resource conservation. The domestication system comprises a domestication field, a light source, a flow making pump, an artificial fish reef and a camera system. The system provided by the invention is used for domesticating the ingestion and predation escape capability of the release fries, enhancing the capability of the artificially propagated proliferation and release fries to adapt to the marine natural environment, improving the survival rate of the release fries, and further improving the proliferation effect of seawater fish release.

The invention discloses a neoseiulus californicus low-temperature storage method, and belongs to the field of agricultural insect biological control. According to the method, the collected neoseiulus californicus homochronous female adults are in split charge into 2ml plastic centrifugal tubes sterilized by two kinds of cold storage media, and the centrifugal tubes subjected to the split charging are respectively placed into cold storage rooms being 4 DEG C, 7 DEG C and 10 DEG C according to different cold storage substrate and cold storage periods, and are covered by black light-tight plastic films. The method provided by the invention has the advantages that the neoseiulus californicus can be stored for a long time at low temperature, under the condition of not influencing the survival rate, the breeding capability and the predation capability similar to those of the neoseiulus californicus which is not subjected to cold storage can be maintained, in addition, the predatory neoseiulus californicus is enabled to be protected from mechanical injury in the conveying process, and the guarantee is provided for the commercialized operation of the local neoseiulus californicus.

The invention relates to a preparation method of a NiMoO4 / MoS2 / NiS nanocomposite. The preparation method is characterized in that raw materials including ammonium molybdate, nickelous acetate and thiourea are solved in 70mL of ultrapure water by taking sodium chloride as a structure directing agent and stirred at the room temperature till being completely dissolved; the solution is transferred to a reaction still of 100mL, and is subjected to reaction for 16-24h at 160-180DEG C; the solution is cooled to the room temperature after reaction, products obtained by centrifugation are washed by ultrapure water for three times and then by waterless ethanol for three times, placed in a vacuum drying box and dried for 12h at 60 DEG C; and the dried products are placed in a vacuum inflated tubular furnace, and calcined for 2h at 400 DEG C with protection of N2 to obtain the NiMoO4 / MoS2 / NiS nanocomposite. The predation method is scientific, reasonable and easy, low in cost and suitable for industrial large-scale production, and the morphology, size and structure of products are controllable.

The invention discloses a crayfish larva breeding method for rice field culture, and relates to the technical field of crayfish breeding. The method comprises the steps of breeding pool building, crayfish larva selection, seed crayfish feeding, crayfish larva incubation, crayfish larva feeding, applicability feeding and crayfish larva choosing. In the process of artificial crayfish larva breeding, directional manual intervention is applied, so that bred crayfish can better adapt to rice field growing environment, the bred crayfish can be matched with the rice field to obtain aquaculture income, and the income of farmers is increased; according to crayfish growing in the rice field, on one hand, predation locust insects and aquatic pest can be increased; on the other hand, rice ear fallen flowers can be adopted as crayfish food intake.

The invention relates to a method for obviously improving the survival rate of exopalaemon carinicauda in the artificial breeding process, in particular to a method for carrying out larva separation and collection by utilizing phototaxis of larvae. The method comprises the following steps of dividing different functional regions in a spawning pool; regulating illumination in different functional regions; and installing a lifting adjustable larva collection net. In the step of dividing the functional regions, a large-aperture screen is utilized to isolate and divide the spawning pool into a parent shrimp action region and a larva collection region; and illumination regulation comprises the steps of covering the parent shrimp action region with a sunshade net and carrying out lure in the larva collection region by a fluorescent lamp. The method has the advantages that division of different functional regions and illumination regulation enable larvae and parent shrimps to be spatially and effectively separated and reduces the predation rate of the parent shrimps for the larvae, so that post-larvae yield is greatly improved; meanwhile, embryo abscission and death of the parent shrimps, which are caused by frequent sorting of the parent shrimps, are also avoided and the survival rate in the breeding process is improved; and the lifting adjustable larva collection net can be used for collecting larvae in due time according to the hatching condition of larvae, so that labor intensity is reduced and the practical value on production is improved.

The invention relates to a full-life-stage recirculating aquaculture system of jellyfish and a breeding method therefor. The full-life-stage recirculating aquaculture system of the jellyfish comprisesa dismountable breeding cylinder, a bottom cylinder, a filtering system, a water pump, an upper water pipe, a lower water pipe or the like. The upper water pipe is used for supply water to the breeding cylinder, and the lower water pipe is used for leading the water in the breeding cylinder out and into the filtering system in the bottom cylinder, so that the breeding water can be recycled between the breeding cylinder and the filtering system. The breeding cylinder provides power for motion and predation of the jellyfish and realizes an oxygenation effect by air holes and / or a porous water spraying pipe, and a porous inclined plate blocks the jellyfish so as to keep the jellyfish in the breeding cylinder, so that mechanical damage caused by jellyfish salvage can be avoided. The full-life-stage recirculating aquaculture system of the jellyfish, and the breeding method therefor solve breeding problems in different stages of a life cycle of the jellyfish, can also greatly save manpowerand time consumed in jellyfish breeding, increase breeding efficiency and are of good application prospect.

The invention relates to a method for preparing rice fertilizer from kitchen waste and mushroom bran and belongs to the technical field of fertilizer manufacturing. Chicken manure is subjected to pyrolysis to obtain charcoal, the charcoal has rich micro-pores, and can provide a habitat for soil microorganisms, protect the soil microorganisms against predation and provide nutrient substances needed for growth of the microorganisms, the charcoal can replenish the content of organic substances of soil and can also effectively preserve water and nutrients, adsorb heavy metals and toxic organic substances in the soil, improve soil fertility and improve the soil structure, the mushroom bran, leftovers and marsh gas liquid contain rich protein, fat, nitrogen extract and mineral substances, during joint compost maturity, bacteria and eggs inside can be killed, and the composted materials are rich in nutrition, and can be absorbed rapidly when acting on rice and meet the requirements for growth of the rice, and contain multiple beneficial bacteria so that decomposition of organic substances in the soil and absorption of the crops can be promoted.

The invention discloses an indoor isolation feeder for spider mites; the indoor isolation feeder comprises a culture dish which is composed of a bottom wall and a lateral wall and is open at one end, host leaves placed in the culture dish and protective isolation parts for preventing the escaping of the spider mites; the protective isolation parts are of wet bar-shaped structures around the leaves. The leaves utilized for feeding the spider mites are capable of preventing the spider mites from escaping and from being drowned; meanwhile, the technical problems in leaf feeding and water isolation feeding can be solved; the indoor isolation feeder for spider mites is simple in structure, does not need to utilize other complex escaping prevention measures and has the characteristics of being good in moisture retention, long in refreshing time of leaves, easy in temperature control, strong in operability and controllable in activity range of insects; the feeding process is simplified and the feeder can be used for performing indoor culturing experiments, indoor fertility and host selectivity experiments of the spider mites, pesticide efficacy and toxicity measurement as well as accurate measurement of predation quantity of natural enemies; the indoor isolation feeder solves the error problem caused by epitrophy and phototropism of insects to the experimental operation and solves the experimental errors caused by partial spider mites remaining at the top of a mite cultivating shade when the mite cultivating shade is utilized to perform an experiment in the prior art; the experimental errors are reduced and the experimental accuracy is improved.

The invention relates to a greenhouse disease-and-insect-pest controlling method using predation mites as a carrier to carry bio-control bacteria. The method includes four parts, namely, bio-control bacterium preparation, predation mite preparation, mixed packaging of the predation mites and the bio-control bacteria, greenhouse disease-and-insect-pest controlling using the predation mites as the carrier to carry the bio-control bacteria. In the method, the bio-control bacteria and the predation mites are required to be stored separately, pure or almost free-of-any-medium predation mites are selected, vermiculite used for packaging media must go through drying treatment, a refrigerated storage temperature is between 1-5 DEG C, when the predation mites are needed to be used as the carrier to carry the bio-control bacteria to control greenhouse diseases and insect pest, the predation mites and the bio-control bacteria are mixed, and a broadcast sowing method is adopted as a releasing method for releasing the predation mites and the bio-control bacteria. By the aid of the greenhouse disease-and-insect-pest controlling method, a potential safety hazard that the bio-control bacteria germinate in a packaging bag can be avoided, and more than 10% prevention effect can be further enhanced.

An effect of reducing sludge amount in a multiple-stage activated sludge method utilizing the predation action of animalcules is stabilized. Organic wastewater is introduced into a first biological treatment tank 1 equipped with aeration means and 70% or more of, preferably 80% or more of, further more preferably 90% or more of the organic components (soluble BOD) is oxidatively decomposed by a common bacterium present in raw water such as Alcaligenes, Pseudomonas, Bacillus, Aerobacter, or Flavobacterium. The treated water in the first biological treatment tank 1 is introduced into a second biological treatment tank 2 and the reduction of the amount of excess sludge is conducted by the oxidative decomposition of remaining organic components, the autolysis of dispersive bacteria and the predation by animalcules. In the second biological treatment tank 2, a fluid bed in which a support C is added so as to increase the holding capacity of the animalcules in the tank is used.

The invention relates to a breeding method and a use method of cantheconidea furcellata for preventing and controlling noorda blitealis, and belongs to the technical field of plant pest control. The purpose of the invention is realized by carrying out manual inducing feeding on cantheconidea furcellata and selecting a reasonable release density. Firstly, the first generation is domesticated and bred, and then inducing feeding is carried out on the second generation for generating the habit of preying noorda blitealis. The second generation of the cantheconidea furcellata is subjected to inducing breeding to becone three-instar, four-instar and five-instar nymphs or adults, and the nymphs or adults are directly released into a moringa oleifera forest for preventing and controlling the noorda blitealis. Plant leaves, filter paper sheets and culture dishes are placed at moringa oleifera branches with a large population density of the noorda blitealis, so that the diffusion and prey searching of cantheconidea furcellata larvae are facilitated. Each cantheconidea furcellata can prey about 21.04 noorda blitealis during the period of growing from a three-instar nymph to an adult, and hasstronger predation performance in an adult period, and the releasing amount can be determined according to the occurrence situation of the noorda blitealis in a field. The breeding method and the usemethod provided by the invention provide an effective biological preventing and controlling method for the damage of the noorda blitealis during the growth process of the moringa oleifera.

A lignocellulose simultaneous saccharification and fermentation method for predation of fuel ethanol belongs to the technical fields of biological resource recycling and biomass energy. The method includes the following steps: (1) conducting irradiation pretreatment on solid biomass; (2) pulverizing the pretreated biomass, and conducting in situ detoxification by using water, organic solvent / water biphasic extraction, sodium hydroxide solution, or sodium borohydride solution; and (3) conducting simultaneous saccharification and fermentation on the detoxified biomass to prepare ethanol. The invention has the advantages that the coupling irradiation pretreatment and simultaneous saccharification and fermentation technology are simple and efficient in operation, and overcome the disadvantages of high energy consumption, large amount of water consumption and easy cause of secondary pollution in the traditional technologies such as dilute acid pretreatment, ionic liquid pretreatment and steam explosion pretreatment.