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Application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria

A technology of plant pathogenic bacteria and myxobacteria, which is applied in the direction of application, bacteria, plant growth regulators, etc., can solve the problems of limited research on the biological control of myxobacteria

Active Publication Date: 2017-07-21
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] At present, there are not many studies on the biological control of myxobacteria at home and abroad, and the above reports basically include all published papers

Method used

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  • Application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria
  • Application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria
  • Application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria

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Experimental program
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Effect test

Embodiment 1

[0036] Myxococcus sp.e-3-1 and Polyangium sp.8#-3 of the present invention are derived from saline-alkali soil in Aksu, Xinjiang.

[0037] The myxobacteria Cystobacter sp. XJ9-1 of the present invention is derived from the virgin forest soil samples in Tam Dao National Park, Vietnam.

Embodiment 2

[0039] Inoculate Myxococcus sp.e-3-1, Polyangium sp.8#-3, Cystobactersp.XJ9-1 into CYE culture medium (10mM MOPS, 10g / L casein, 5g / L yeast extract, 8mM MgSO 4 , the solvent is water, pH 7.6), 150rpm, 30 ℃ for 3d, and then MMC buffer (10mM MOPS, 4mM MgSO 4 , 2mM CaCl 2 , the solvent is water, pH 7.6) washed and diluted to 1×10 11 cell / ml to obtain the myxobacteria bacterial liquid.

[0040] 分别接种各植物病原细菌(Rhizobium radiobacter GIM 1.274,Pseudomonassyringae GIM 1.330,Acidovorax avenae subsp avenae JCM 20985,Rhodococcusfascians NBRC 12155,Burkholderia cepacia GIM 1.450,Curtobacteriumflaccumfaciens GIM 1.343,Erwinia persicina GIM 1.331,Arthrobacter ilicis JCM12267,Erwinia chrysanthemi GIMT 1.178, Pantoea stewartii subsp.stewartii LMG2715,Ralstonia solanacearum GIM 1.70,Rhizobacter dauci DSM 11587,Sphingomonassuberifaciens JCM 8521,Xanthomonas campestris JCM 20466,Xylellafastidiosasubsp.fastidiosa LMG 17159,Streptomyces scabiei JCM 7914,Bacillus megaterium GIM1.270,Clavibacter michi...

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Abstract

The invention discloses application of myxobacteria in preparation of drugs for predation and inhibition of plant pathogenic bacteria. The Myxococcus sp.e-3-1, Polyangium sp.8#-3, Cystobacter sp.XJ9-1 involved in the invention on one hand can prey and lyse various plant pathogenic bacteria, and on the other hand can produce active natural products inhibiting Erwinia persicina, thus having important application value. The Myxococcus sp.e-3-1, Polyangium sp.8#-3 and Cystobacter sp.XJ9-1 can be applied in biological control, drug development and other aspects.

Description

[0001] Technical field: [0002] The invention belongs to the fields of microbiology, biological control, natural product chemistry and pharmacology, and specifically relates to three strains of myxobacteria capable of predating and inhibiting plant pathogenic bacteria, and their application in preparing medicines for predating and inhibiting plant pathogenic bacteria. [0003] Background technique: [0004] Plant diseases endanger agricultural production and cause economic losses, and the research on their control is of great significance. Biological control technology has the advantages of no pollution to the environment, good safety for humans and other organisms, long-lasting control effect, no residue, strong specificity for killing diseases, easy coordination with other control measures, energy saving, etc., so it has broad development future. In today's world, people pay more and more attention to environmental protection, and the demand for "pollution-free products" co...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 李安章朱红惠张鲜姣姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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