117 results about "Pantoea" patented technology

Microbiological agents are provided for control of certain diseases of wheat and other cereals caused by Fusarium species, including Fusarium head blight of wheat and other cereals. These agents can also improve yield of wheat plants and cereals. The agents are novel isolates of Pantoea agglomerans and of Bacillus megaterium that exhibit the property of inhibiting fungal pathogens, particularly those produced by Fusarium species. Biocontrol compositions, and methods of using them to control plant pathogen development on wheat and cereal plants and for increasing plant yield, are also provided. The biocontrol compositions comprise a mixture of at least one microorganism selected from the group consisting of Pantoea agglomerans and Bacillus megaterium.

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

Six bacterial strains: Bacillus simplex strain 03WN13, Bacillus simplex strain 03WN23, Bacillus simplex strain 03WN25, Pseudomonas koreensis strain 10IL21, Pantoea agglomerans strain 10IL31, and Pseudomonas lini strain 13IL01, are superior antagonists of Phytophthora erythroseptica Pethybr., the causative agent of pink rot on potatoes. These bacterial strains are effective for suppression and control of pink rot on potatoes.

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genus Pantoea, Enterobacter, Klebsiella or Erwinia, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.

The invention discloses pantoea for degrading organic phosphorus and inorganic phosphorus and application of the pantoea. The preservation number of the pantoea (Pantoea sp.)S-32 in a general microorganism center of the China Committee for Culture Collection of Microorganisms is CGMCC No.10017. According to the pantoea, the phosphorus solubility for degrading calcium phosphate reaches 24.19% and is equal to 3.64 times of that of bacillus megatherium As1,223; the phosphorus solubility for degrading lecithin reaches 2.47% and is equal to 1.46 times of that of bacillus megatherium As1,223; the pantoea has relatively strong environmental suitability and can adapt to relatively wide ranges of temperature, pH value and salinity. Experiments prove that the pantoea (Pantoea sp.)S-32 CGMCC No.10017 can serve as a strain resource of microbial fertilizers so as to increase the crop yield and accelerate the ecological restoration of soil of mining areas.

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to have glycerol kinase in which feedback inhibition by fructose-1,6-bisphosphate is desensitized, thereby having enhanced ability to utilize glycerol.

The invention discloses a complex microbial agent for low-temperature methane fermentation. According to the ccomplex microbial agent, fermentation broths obtained by fermenting pseudomonas sp. CGMCC (China General Microbiological Culture Collection) No.4764, pantoea agglomerans CGMCC No.4765 and pantoea agglomerans are uniformly mixed according to the weight ratio of 1:1:1, so that a liquid formis obtained. The complex microbial agent disclosed by the invention can be directly fed into a methane tank, so that the methane tank can be used for normally producing gas in winter; and in addition, the complex microbial agent has a wide application value.

This invention provides a strain for producing 2, 3-butanediol, and its application as anti-UV and anti-oxidation agent in cosmetics. The strain with strong UV tolerance is separated from mangrove, and can produce 2, 3-butanediol. The strain has such advantages as wide nutrition range, short culture period, low cost and high yield. 2, 3-butanediol has good anti-UV and anti-oxidation effects, and can eliminate active oxygen free radicals and block lipid peroxidation, thus can be used as anti-UV and anti-oxidation agent in cosmetics.

The invention discloses a sucrose isomerase mutant with improved stability and a construction method thereof, and belongs to the field of gene engineering and enzyme engineering. On the basis of high-enzyme-activity dispersed pantoea-derived sucrose isomerase, two mutant strains V280L and S499F with improved single-point thermal stability and a combined mutant V280L/S499L are obtained through screening. The thermal stability of the three mutants is obviously improved, and the enzyme activity is not influenced. The mutants provided by the invention are more suitable for industrial production than natural sucrose isomerase, and have very huge application prospects and industrial values.

The invention discloses a microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone. The method includes the following steps: (1), culturing scattered pantoea, inoculating a triangular bottle filled with a seed culture medium with a scattered pantoea strain, and oscillating at 180-200r/min under the conditions of pH being 6.5 and temperature being 30-34 DEG C for culture for 10-18h to obtain seed liquid; (2), fermenting in a seed tank; (3), obtaining 3-hydroxy beta-ionone and beta-ionone. By searching fermentation culture media, an optimal fermentation culture medium capable of efficiently degrading lutein is obtained, and 3-hydroxy beta-ionone and beta-ionone which have aromatic substances can be generated. The 3-hydroxy beta-ionone and beta-ionone are important aromatic substances, and tobacco quality can be improved greatly by adding a small amount of the aromatic substances into tobacco. The microbial-conversion-based method has huge application prospect in utilizing biologically-degraded lutein and generating natural aromatic substances.

The invention discloses a rhodobacter sphaeroides engineering strain for producing beta-carotene and a construction method thereof. The method includes first optimizing a codon of a lycopene cyclase gene crtYPa derived from pantoea agglomerans to obtain an opt crtYPa gene, then replacing an endogenous phytoene three-step dehydrogenase gene crtI3 of rhodobacter sphaeroides with the opt crtYPa genecontaining a promoter prrnB and a phytoene four-step dehydrogenase gene crtIPa gene derived from the pantoea agglomerans in a traceless mode, then knocking out an endogenous neurosporene hydroxylase gene crtC of the rhodobacter sphaeroides and a 6-phosphoglucose dehydrogenase gene zwf and finally knocking out an endogenous 1-deoxyxylulose-5-phosphate synthase gene dxs of the rhodobacter sphaeroides with integration expression at zwf position to obtain the rhodobacter sphaeroides engineering strain for producing the beta-carotene. The strain is cultured by fermentation, and the content of the beta-carotene can reach 30mg/g DCW.

The invention provides pantoea agglomerans and application thereof. A preservation number of the pantoea agglomerans is CCTCC NO: M2015563. The thallus of the pantoea agglomerans is rod-shaped and is negative when being treated by Gram staining; the pantoea agglomerans can be prepared into a liquid bacterial agent or wettable powder and is used for preventing and treating wheat scab and reducing the content of wheat gibberellin; in a process of solving toxin pollution, no toxic byproducts are generated and secondary pollution is not caused, so that the quality of agricultural products is improved and the edible safety of people and livestock is ensured.