34 results about "Pantoea" patented technology

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

It is considered that diabetes-associated dementia is mainly caused by insulin resistance and relative insulin deficiency in the brain. Delivery of drugs to the brain is inhibited by the blood-brain barrier, and therefore, there has been so far no drug that provides excellent drug delivery to the brain. Therefore, there has been no wonder drug for diabetes-associated dementia. LPSs have a high molecular weight and therefore do not pass through the blood-brain barrier. It is possible to provide a composition for a drug, food, or the like that has the effect of preventing and treating impairment due to diabetes-associated dementia, by means of a lipopolysaccharide derived from a bacterium belonging to the genus Pantoea. A lipopolysaccharide derived from a bacterium belonging to the family Enterobacteriaceae is confirmed to be safe when used for oral or transdermal administration in the form of food, cosmetics, feed, or the like, and thus can be expected to provide prophylactic and therapeutic effects with a low risk of side effects.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The invention discloses a Pantoea sp. ZC11L and an application thereof. The pantoea sp. ZC11L is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 16, 2023, and the preservation number is CGMCC No.29038. The pantoea sp. ZC11L has the advantages that the preservation number is CGMCC No.29038; the yield of indoleacetic acid (IAA) after 48-72 h of fermentation is 100-500 [mu] g / mL, the germination and growth promoting rate of coptis chinensis seeds is increased by 70-80%, and a microbial agent prepared from the pantoea ZC11L can be used for breeding and seedling raising of coptis chinensis and has the advantages of being efficient, safe, environmentally friendly and the like.

The application discloses application of combined inoculation of rhizosphere phosphorus solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms.The combined inoculation of acinetobacter and pantoea provided by the application can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, and promotes the utilization of phosphorus.The combined inoculation of acinetobacter and pantoea has significant soil phosphorus solubilizing capacity and plant growth promotion effect when the ratio of acinetobacter to pantoea is (4-6):(6-4), and can be applied to improving soil phosphorus utilization rate and promoting plant growth, and has important agricultural and environmental values.

The pantoea agglomerans SE16 is classified and named as pantoea agglomerans, and is preserved in the China General Microbiological Culture Collection Center on October 17, 2024, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 32246. The bacterial strain is applied to strengthening phytoremediation of heavy metal pollution, improving soil nutrients, promoting plant growth and strengthening phytoremediation of pollutants. The strain has the functions of dissolving phosphorus, fixing nitrogen, producing iron carriers, producing indoleacetic acid, ACC deaminase and cellulase, resisting acid, resisting salt and the like, and also can tolerate various heavy metals, polycyclic aromatic hydrocarbons and combined pollution of the heavy metals and the polycyclic aromatic hydrocarbons; by promoting the growth of the sudangrass, improving the photosynthesis of the sudangrass, relieving the oxidation pressure of the sudangrass and changing the composition of soil microbial communities, the toxicity of chromium to the sudangrass is relieved, and the method has practical popularization and application values.

The present application relates to the field of environment, the utilization of phosphate rock resources and the field of agricultural biotechnology, and in particular to a kind of hydroxyapatite-degrading phosphate-solubilizing bacterial agent and its preparation method, biological bacterial fertilizer and application, the hydroxyapatite-degrading phosphate-solubilizing bacterial agent includes phosphate-solubilizing bacteria, hydroxyapatite and culture medium, the phosphate-solubilizing bacteria is Pantoea agglomerate CT3.By using hydroxyapatite to prepare the hydroxyapatite-degrading phosphate-solubilizing bacterial agent, the full use of phosphate rock resources can be greatly promoted, and the dissolution of insoluble phosphorus in phosphate rock is realized.The hydroxyapatite-degrading phosphate-solubilizing bacterial agent combines Pantoea agglomerate CT3 with hydroxyapatite, which can utilize the phosphate-solubilizing ability of Pantoea agglomerate CT3 and fully utilize the phosphorus resources of hydroxyapatite.The hydroxyapatite-degrading phosphate-solubilizing bacterial agent has good prospects in the preparation of biological bacterial fertilizer, application in agricultural soil improvement, application in phosphate rock resource recovery and application in promoting wheat growth.

The invention relates to application of spirodela polyrhiza and microorganism combination in repairing a cadmium-polluted water body and reducing the cadmium content of rice grains in a cadmium-polluted rice field. The preparation method comprises the following specific steps: firstly, respectively preparing a bacillus bacterium suspension, an enterobacter bacterium suspension and a pantoea bacterium suspension; mixing the three bacterial suspensions in proportion to obtain a mixed bacterial suspension; then the mixed bacterial suspension and spirodela polyrhiza are prepared according to the proportion and then added into a cadmium-polluted water body to be remedied or a cadmium-polluted rice field to be remedied, and spirodela polyrhiza and microorganisms form a spirodela polyrhiza-functional flora symbiotic system; the microorganisms convey the cadmium to the leaves through adsorption, leaf surface sedimentation conversion or transpiration; and finally, the spirodela polyrhiza is treated in a centralized manner, so that the cadmium-polluted water body is repaired or the cadmium content of rice grains is reduced. By means of the method, the cadmium-polluted water body can be well repaired, and the cadmium content of rice grains in the cadmium-polluted rice field can be effectively reduced.

The invention provides a composition containing acetobacter saxatilis and pantoea azototrophicus and application of the composition. The composition provided by the invention comprises acetobacter sativus or progeny thereof and pantoea azototrichum or progeny thereof; the registration number of the acetobacter breviscapus in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36149; the registration number of the nitrogen-fixing pantoea in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36150. The invention further discloses a preparation method of the nitrogen-fixing pantoea. The acetobacter sativus and the nitrogen-fixing pantoea or the composition of the acetobacter sativus and the nitrogen-fixing pantoea can be used for reducing emission of nitrogen-containing gas in the livestock breeding process and the livestock manure (normal state and composting) treatment process, and has a good application prospect.

The application discloses a biocontrol pantoea agglomerans KBA33 for inhibiting botryosphaeria dothidea and application thereof. The strain KBA33 disclosed by the application can effectively inhibit botryosphaeria dothidea at 16 DEG C and 28 DEG C, and can inhibit botryosphaeria dothidea through cell-cell contact.

The invention belongs to the technical field of microorganisms, and discloses a Pantoea agglomerans strain with high glutamic acid yield, the name of the Pantoea agglomerans strain is PA-GLU-10, the classification name is Pantoea agglomerans, the preservation number is GDMCC 65358, the preservation date is October 28, 2024, and the preservation unit is Guangdong Microbial Culture Collection Center, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The invention provides Pantoea agglomerans, the Pantoea agglomerans can be used for fermentation production of glutamic acid, has the advantages of tolerance to relatively low pH in a fermentation process, high glutamic acid production speed, suitability for continuous culture and the like, overcomes the problems of poor acid resistance, high cost and the like of an existing fermentation strain, and is expected to reduce the production cost of glutamic acid.

The invention discloses a preparation method and application of a radix scrophulariae sweating processed product. The method comprises the following steps: raw material treatment: selecting fresh radix scrophulariae, and removing non-medicinal parts; processing: regulating and controlling microbial communities in radix scrophulariae in a manner of combining steaming, drying and sweating; the method specifically comprises any one of direct drying at 60 DEG C, repeated sweating for 3 days to dry after drying at 60 DEG C to half-dry, direct repeated sweating for 3 days to dry, direct drying at 60 DEG C after steaming, direct drying at 60 DEG C after steaming to half-dry and repeated sweating for 3 days to dry, and direct repeated sweating for 3 days to dry at 60 DEG C after steaming. According to the method, the abundance change of key microorganisms such as pseudomonas aeruginosa, bacillus, pantoea and cladosporium is directionally promoted through a specific processing technology, and a new way is provided for quality standardization and quality improvement of radix scrophulariae processed products.

The invention provides a bio-fertilizer for promoting plant growth and enriching selenium and a preparation method thereof, and belongs to the technical field of bio-fertilizers, the bio-fertilizer is based on a dried and sterilized mixed organic matter and a direct vat set pantoea inoculant, the mass fraction of N in the mixed organic matter is 4-8%, the mass fraction of P2O5 in the mixed organic matter is 1.6-3.3%, and the mass fraction of K2O in the mixed organic matter is 1.6-3.3%. The selenium-enriched biological fertilizer for promoting plant growth is characterized in that the selenium-enriched biological fertilizer for promoting plant growth is prepared by mixing and fermenting mixed organic matters, calcium superphosphate, sodium selenite and a direct-vat-set pantoea agglomerans fungicide, the pantoea agglomerans AKFX-001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122. The strain disclosed by the invention has the effects of resisting high-concentration selenium, converting inorganic selenium into organic selenium and promoting plant growth at the same time, and a single strain realizes the functions which can be realized by a previous mixed strain, so that the strain has a very good market prospect.

The invention relates to the technical field of microorganisms, in particular to pantoea BG4-1 with a growth promoting effect and application of the pantoea BG4-1. The pantoea BG4-1 is preserved in the China General Microbiological Culture Collection Center on June 20, 2025, and the preservation number of the pantoea BG4-1 is CGMCC NO.34960. The pantoea BG4-1 has relatively strong capabilities of dissolving phosphorus, dissolving potassium and producing IAA (Indoleacetic Acid), and has a remarkable growth promoting effect on capsicum and arabidopsis thaliana. According to the invention, a high-quality strain resource is provided for developing a novel efficient biological phosphorus-potassium fertilizer, the method has important meanings for improving the utilization rate of phosphorus and potassium elements in soil, reducing the application of chemical phosphorus-potassium fertilizers and promoting the sustainable development of agriculture, meanwhile, a new way is provided for resource utilization of invasive plants, and the ecological management concept of'taking harm as benefit 'is favorably realized.

The invention relates to the technical field of microorganisms, and discloses pantoea H1 and a demonstration method and application thereof in agriculture assistance, the pantoea H1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36875. The invention further provides a microbial agent containing the bacterial strain, a preparation method and application of the bacterial strain in crop planting, the bacterial strain has efficient dual phosphorus solubilizing capacity, indissolvable inorganic phosphorus can be dissolved, organophosphorus can be mineralized, and the effectiveness of soil phosphorus is improved; meanwhile, the microbial agent has excellent saline-alkaline tolerance, can still keep high activity under adversity, and by applying the microbial agent in the seedling stage of crops, the rhizosphere microenvironment can be remarkably improved, root development and plant growth of the crops such as corn and mung beans can be promoted, the emergence rate and yield of the crops in soil such as saline-alkaline land can be increased, and the crop yield can be increased. And an excellent microbial germplasm resource is provided for activating soil nutrients, reducing weight and improving efficiency.

This invention belongs to the field of microbial technology, and specifically relates to a highly efficient phosphate-solubilizing bacterium, its preparation method, and its application. The phosphate-solubilizing bacterium is named Pantoea cypripediP11, with the biological preservation number CCTCC NO: M2023159. The highly efficient phosphate-solubilizing bacterium Pantoea cypripediP11 proposed in this invention can convert insoluble phosphorus into soluble phosphorus at a maximum content of 663.59 mg / L, which can increase the available phosphorus content in the soil by 1.46 times. This is stronger than the phosphate-solubilizing ability of the existing phosphate-solubilizing bacterium Burkholderia ubonensis, and it can also produce indoleacetic acid and secrete siderophores to promote plant root growth.

The invention discloses an application of Pantoea ananatis, and relates to the technical field of microorganisms. Aiming at the defects of the existing obesity intervention means, the invention provides a brand-new solution: Pantoea anatitis adapted to a high-altitude environment is used as a core functional strain, through an intestinal flora driving mechanism, the expression of UCP1 protein in brown adipose tissue (BAT) is activated, the heat production function of the BAT is enhanced, and meanwhile, the storage of white adipose tissue (WAT) and the level of liver triglyceride (TG) are reduced; the weight-losing effect is realized. The strain can play a role through an'intestinal flora-BAT 'axis without cold exposure, has no obvious influence on liver functions, and is excellent in safety. The invention discloses a flora-mediated non-cold-dependent BAT activation way for the first time, can be used for preparing products such as weight-reducing medicines and health-care foods, has the advantages of mechanism innovation, exact effect and wide application, and provides a new technical choice for obesity intervention.

The invention provides a bacteriophage CM23S1 with a function of splitting pantoea agglomerans and application of the bacteriophage CM23S1, and belongs to the technical field of biological control. The phage is named as CM23S1, the whole length of a genome sequence is 92308 bp, the phage is preserved in the China General Microbiological Culture Collection Center on October 21, 2025, and the preservation number is CGMCC No.46684; the invention provides a method for separating and purifying the bacteriophage and preparing a biological preparation. The bacteriophage has relatively high stability and splitting property on pantoea agglomerans. An indoor bacteriostasis test shows that the bacteriophage has an obvious bacteriostasis effect on pantoea agglomerans, and a potted wheat test also proves that the bacteriophage can be used for preventing and treating plant diseases caused by pantoea agglomerans.

The invention provides a pantoea widely distributed strain YC2472 and application thereof, and belongs to the technical field of biological control. The taxonomic name of the YC2472 strain is Pantoea vagans, and the YC2472 strain is preserved in Guangdong Microbiological Culture Collection Center, and the address is Institute of Microbiology, Guangdong Academy of Sciences, Floor 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou; the preservation date is July 4, 2024; the preservation number is GDMCC No: 64827. According to the invention, a bacterial strain YC2472 with stable and significant antagonistic activity on the pathogenic bacteria is separated and screened from rhizosphere soil of tobacco, and the bacterial strain YC2472 is identified as Pantoea vulgaris. Retrieval of domestic and foreign literatures shows that the biocontrol activity of Pan.vans on phytophthora nicotianae is not reported, so that the innovativeness and potential application value of the Pan.vans are highlighted.

The invention discloses a complex microbial inoculant for onions and a preparation method of the complex microbial inoculant. The complex microbial inoculant comprises acinetobacter JQ-MY-41 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.32731, pantoea JQ-MY-42 with the preservation number of CGMCC NO.32732 and alcaligenes YC-342 with the preservation number of CGMCC NO.32733, and the optimal mass ratio of the acinetobacter JQ-MY-41 to the pantoea JQ-MY-42 to the alcaligenes YC-342 to the alcaligenes YC-342 is 30: 25: 30. The preparation method comprises the steps of strain activation, strain culture, thallus collection, strain compounding, carrier addition, drying and the like. The three strains achieve synergistic growth promotion through different mechanisms such as phosphorus solubilization, nitrogen fixation and IAA production, and compared with a control group, the onion plant height is increased by 37.5%, the stem diameter is increased by 39.0%, the total biomass is increased by 103.7%, and the total chlorophyll amount is increased by 69.5%; the problems that an existing microbial agent is single in function and limited in growth promoting effect are solved, and technical support is provided for green and efficient cultivation of onions.

The invention discloses an atractylodes macrocephala root rot biocontrol bacterium and application thereof, and belongs to the technical field of plant disease biocontrol. The rhizoma atractylodis macrocephalae rhizosphere bacterium is separated from healthy rhizosphere soil of a compositae plant rhizoma atractylodis macrocephalae by adopting a rhizosphere bacterium separation and purification technology, is classified and named as Pantoea rodsii RSB15, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M20251954, and the preservation date is September 1, 2025. The invention also discloses application of the bacterium: the bacterium can effectively inhibit the growth of pathogenic bacteria of bighead atractylodes rhizome root rot, and the inhibition rate of plate confrontation culture reaches 20.57%; the morphological structure of pathogenic fungi can be destroyed. The bacterium provided by the invention has an obvious effect of preventing and treating bighead atractylodes rhizome root rot and provides a wide application prospect in the field of biological prevention and treatment of bighead atractylodes rhizome fungal diseases.

The invention discloses corn endophytic pantoea dispersa X24004 and an application of the corn endophytic pantoea dispersa X24004. The preservation number of the Pantoea dispersa X24004 is CGMCC (China General Microbiological Culture Collection Center) No. 34510, and the preservation number of the Pantoea dispersa X24004 is CGMCC No. 34510. The pantoea dispersa X24004 has a relatively strong antagonistic effect on a plurality of pathogenic fungi such as fusarium graminearum, fusarium verticillioides, pythium aphanidermatum stem rot pathogenic bacteria and the like, has high efficiency and broad spectrum on prevention and treatment of plant fungal diseases, and also has a promoting capability on growth of corn plants; the biological agent is a broad-spectrum biological agent, a biological bacterial fertilizer and a seed treatment agent with good development and application prospects.

The application provides a widely distributed bacteria strain YC2472 and an application thereof, and belongs to the technical field of biological control. Pantoea vagans The taxonomic name of the YC2472 strain is Pantoea vagans, the preservation unit is the Guangdong Microbial Culture Collection Center, the address is the 5th floor of the No. 59 building of the Guangzhou Martyrs' Road 100 Courtyard, the Institute of Microbiology of the Guangdong Academy of Sciences, the preservation date is July 4, 2024, and the preservation number is GDMCC No: 64827. The application screens a bacteria YC2472 with stable and significant antagonistic activity to the pathogenic bacteria from tobacco rhizosphere soil, and the bacteria YC2472 is identified as Pantoea vagans. Pantoea vagans Through retrieval of domestic and foreign literatures, Pan.vagans the biological control activity of the YC2472 strain to the tobacco Phytophthora has not been reported, so that the application highlights the innovation and potential application value.

The present application belongs to the field of bioengineering, and relates to an engineering strain, in particular to a pantoea that degrades vetiverol and application. The preservation number of the strain is CGMCC NO.30671. The pantoea that degrades vetiverol in the present application can convert vetiverol, an intermediate product in ambergris, into a friendly ambergris substance, and the degradation rate of vetiverol is above 65.99% within 72 h. The pantoea is used for fermentation of tobacco leaves, which is helpful to improve the quality of tobacco leaves and improve the sensory quality of cigarettes.

The invention belongs to the technical field of soil improvement, and particularly relates to a biological conditioner for improving coastal salinized soil and promoting crop growth and a preparation method thereof.The biological conditioner is prepared from, by weight, 40-50 parts of acid activated porous biochar, 10-20 parts of compound microorganism bacterium liquid, 20-25 parts of water retention type slow release nitrogen fertilizer and 5-10 parts of potassium sulfate; the compound microorganism bacterial liquid is obtained by mixing a P.megatherium RS2-15 bacterial liquid, a Bacillus flexus RC2-7 bacterial liquid, a P.putida M1-38 bacterial liquid and an Acinetobacter picei R2-3 bacterial liquid which are equal in volume. The compound microorganism bacterium liquid can improve soil nutrients and soil enzyme activity and promote crop growth; the acid-activated porous biochar can regulate the granular structure of the soil, adsorb salt ions in the soil and improve the saline-alkaline state of the soil; the water-retaining slow-release nitrogen fertilizer can retain water for soil and also can slowly release the nitrogen fertilizer.

The application provides a biological fertilizer for promoting plant growth and selenium enrichment and a preparation method thereof, and belongs to the technical field of biological fertilizers.The biological fertilizer is based on dry sterilized mixed organic matter and a direct-throwing group-forming pantoea agens, and the mass fractions of N, P2O5 and K2O in the mixed organic matter are 4-8%, 1.6-3.3% and 1.6-3.3% respectively, and the biological fertilizer is characterized in that: the biological fertilizer for promoting plant growth and selenium enrichment is prepared by fermenting the mixed organic matter, superphosphoric acid calcium, sodium selenite and the direct-throwing group-forming pantoea agent after mixing, wherein the group-forming pantoea AKFX-001 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 36122.The strain has the effects of resisting high-concentration selenium, converting inorganic selenium into organic selenium and promoting plant growth, and the single strain can realize the functions that can only be realized by mixed strains in the prior art, and has a good market prospect.

The present invention discloses a mutant strain of Pantoea algina without pigment production, wherein the mutant strain is Pantoea algina. Pantoea alhagi XK‑11 was used as the starting strain, and the phytoene synthase gene was knocked out in the XK‑11 genome. crt The present invention also discloses the application of the engineered bacteria for fermentation and preparation of Pantoea acuminata exopolysaccharide. Compared with the starting strain XK-11, the engineered bacteria XK-11Δ crt B. The purification process of preparing extracellular polysaccharides uses less ethanol, and the color of the polysaccharide after drying is pure white, which significantly reduces the production cost and improves the quality of extracellular polysaccharides. It can be used in the development of daily chemical moisturizing, food thickening, medical gel and other products.

The invention provides a method for increasing the content of available phosphorus in soil by using a phosphate solubilizing bacterium combination, and belongs to the technical field of soil improvement. The method for increasing the available phosphorus content of the acid soil by using the phosphate solubilizing bacteria comprises the following steps: (1) selecting a plurality of microorganisms with a phosphate solubilizing effect; (2) adding the microorganisms obtained in the step (1) into soil; the microorganisms comprise 2-8 of bacillus megatherium, acinetobacter, pseudomonas, proteus, rhizobium, paenibacillus, bacillus cereus and pantoea. Different phosphate-solubilizing microorganism combinations are utilized, the influence of the exogenously added phosphate-solubilizing bacteria on soil available phosphorus, organic phosphorus mineralizing enzymes, phosphorus conversion functional genes, a microorganism interaction network and key species is researched, and an effective soil management strategy based on phosphate-solubilizing bacteria diversity regulation and control is provided for agricultural production. The utilization efficiency of soil phosphorus is improved; and sustainable agricultural development is promoted.

The invention relates to a beta-hairpin defense peptide as well as a derivative and application thereof, the beta-hairpin defense peptide is Jz-Def, and the amino acid sequence of the beta-hairpin defense peptide is as shown in SEQ ID No.1, specifically as follows: YCRRVCGRKRCFTYCRGK. The derivative of the beta-hairpin defensive peptide is defensive peptide Jz-M3, and the amino acid sequence of the defensive peptide Jz-M3 is as shown in SEQ ID No.2, specifically as follows: RWCRRFCRNGRCVRYCWR. The invention further discloses application of the beta-hairpin defense peptide and / or the derivative of the beta-hairpin defense peptide in preparation of bacteriostatic and bactericidal drugs or products. The beta-hairpin defensive peptide Jz-Def and the derivative defensive peptide Jz-M3 of the beta-hairpin defensive peptide Jz-Def and the derivative defensive peptide Jz-M3 of the beta-hairpin defensive peptide Jz-Def can kill clinical drug-resistant bacteria gram-positive pathogenic bacteria, gram-negative pathogenic bacteria and fungi at The bacillus amyloliquefaciens has strong antibacterial and bactericidal properties on providencia chinensis and other pathogenic bacteria in aquaculture and enterococcus faecalis, salmonella enterica and other putrefying bacteria in food, has very strong broad-spectrum antibacterial properties, has very strong high-temperature stability in antibacterial and bactericidal properties, has low hemolytic activity, and can be used for preparing the bacillus amyloliquefaciens. The composition has no obvious inhibiting and killing effect on phytobacterium and saccharomyces cerevisiae, and is high in safety.

The invention discloses a pichia pastoris engineering strain for producing zeaxanthin. The invention further discloses a method for screening the beta-carotene hydroxylase CrtZ suitable for the pichia pastoris. The invention also discloses a method for producing zeaxanthin and application of the engineering strain in production of zeaxanthin. According to the present invention, the beta-carotene hydroxylase CrtZ derived from Pantoea stewartii is adopted, and T84S and M92T site-directed mutagenesis is further adopted to obtain the CrtZ mutant, such that the problem of insufficient catalytic kinetics performance of the enzyme in Pichia pastoris is effectively solved; according to the invention, key enzymes CrtZ and CrtY are modularly assembled by using an RIAD / RIDD protein interaction tag, so that a multi-enzyme compound with a specific stoichiometric ratio is formed, and carbon metabolic flux is effectively guided to be directionally converted into a final product zeaxanthin.