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48 results about "Pantoea" patented technology

Pantoea is a genus of Gram-negative bacteria of the family Erwiniaceae, recently separated from the genus Enterobacter. This genus includes at least 20 species. Pantoea bacteria are yellow pigmented, ferment lactose, are motile, and form mucoid colonies. Some species show quorum sensing ability that could drive different gene expression, hence controlling certain physiological activities.

Selenium-resistant pantoea agglomerans and application thereof

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.
Owner:ANKANG SELENIUM-ENRICHED PROD R&D CENT

Application of Alzheimer's disease intestinal flora biomarker combination in preparation of AD detection product

The invention provides application of an Alzheimer's disease intestinal flora biomarker combination in preparation of a product for detecting AD (Alzheimer's disease). The marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, clostridium erysipelas, helicobacter, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas, and is characterized in that the marker combination comprises ackermania muciniphila, helicobacter hispiduli, dunaliella, pantoea, other fungus, lactobacillus, enterobacter, laryngobacter, clostridium and undefined genus of clostridium erysipelas. And the composition is any one or a combination of at least two of bacillus pumilus and odorobacter. According to the present invention, the abundance level of the intestinal flora biomarker combination is adopted as the detection index, and the kit is used for the early auxiliary diagnosis of the Alzheimer's disease, has characteristics of high detection precision, convenience, rapidness, safety and non-invasive property, and provides important clinical guiding significance for the auxiliary diagnosis of AD-related indexes.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI

Prophylactic drug and therapeutic drug for diabetes-associated dementia

It is considered that diabetes-associated dementia is mainly caused by insulin resistance and relative insulin deficiency in the brain. Delivery of drugs to the brain is inhibited by the blood-brain barrier, and therefore, there has been so far no drug that provides excellent drug delivery to the brain. Therefore, there has been no wonder drug for diabetes-associated dementia. LPSs have a high molecular weight and therefore do not pass through the blood-brain barrier. It is possible to provide a composition for a drug, food, or the like that has the effect of preventing and treating impairment due to diabetes-associated dementia, by means of a lipopolysaccharide derived from a bacterium belonging to the genus Pantoea. A lipopolysaccharide derived from a bacterium belonging to the family Enterobacteriaceae is confirmed to be safe when used for oral or transdermal administration in the form of food, cosmetics, feed, or the like, and thus can be expected to provide prophylactic and therapeutic effects with a low risk of side effects.
Owner:SOMA +1

Bacillus safensis AHAU-WU410 and application thereof in prevention and treatment of corn bacterial wilt and promotion of corn plant growth

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.
Owner:ANHUI AGRICULTURAL UNIVERSITY

A recombinant Pantoea algina and its application in fermentation production of exopolysaccharides

ActiveCN120330120BBacteriaHydrolasesMicrobiologyPantoea
The present invention discloses a recombinant Pantoea alpina strain and its use in the fermentation production of exopolysaccharides. The recombinant Pantoea alpina strain has the gene mdoG encoding a transcription factor knocked out; wherein the amino acid sequence of the transcription factor is shown in SEQ ID NO.2. The present invention constructs a recombinant strain with high exopolysaccharide production using CRISPR technology. The gene encoding the transcription factor can be used to direct the improvement of the exopolysaccharide-producing strain, making its exopolysaccharide production significantly higher than that of the wild-type strain, laying the foundation for further metabolic engineering of Pantoea alpina strain to produce polysaccharides. The recombinant strain construction method provided by the present invention is simple and easy to use. The exopolysaccharide produced by the recombinant Pantoea alpina strain constructed by the present invention has a static viscosity of 10.32 to 24.11 Pa·s at room temperature for a 10 g / L exopolysaccharide aqueous solution prepared using the exopolysaccharide, showing good application prospects.
Owner:NANJING TECH UNIV

Pantoea sp. ZC11L and application thereof

The invention discloses a Pantoea sp. ZC11L and an application thereof. The pantoea sp. ZC11L is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 16, 2023, and the preservation number is CGMCC No.29038. The pantoea sp. ZC11L has the advantages that the preservation number is CGMCC No.29038; the yield of indoleacetic acid (IAA) after 48-72 h of fermentation is 100-500 [mu] g / mL, the germination and growth promoting rate of coptis chinensis seeds is increased by 70-80%, and a microbial agent prepared from the pantoea ZC11L can be used for breeding and seedling raising of coptis chinensis and has the advantages of being efficient, safe, environmentally friendly and the like.
Owner:MICROBIOLOGY INST OF SHAANXI

Application of combined inoculation of rhizosphere phosphorus solubilizing bacteria in plant growth promotion and soil nutrient improvement

The application discloses application of combined inoculation of rhizosphere phosphorus solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms.The combined inoculation of acinetobacter and pantoea provided by the application can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, and promotes the utilization of phosphorus.The combined inoculation of acinetobacter and pantoea has significant soil phosphorus solubilizing capacity and plant growth promotion effect when the ratio of acinetobacter to pantoea is (4-6):(6-4), and can be applied to improving soil phosphorus utilization rate and promoting plant growth, and has important agricultural and environmental values.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

Endophytic bacterium pantoea agglomerans SE16 and application thereof

The pantoea agglomerans SE16 is classified and named as pantoea agglomerans, and is preserved in the China General Microbiological Culture Collection Center on October 17, 2024, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 32246. The bacterial strain is applied to strengthening phytoremediation of heavy metal pollution, improving soil nutrients, promoting plant growth and strengthening phytoremediation of pollutants. The strain has the functions of dissolving phosphorus, fixing nitrogen, producing iron carriers, producing indoleacetic acid, ACC deaminase and cellulase, resisting acid, resisting salt and the like, and also can tolerate various heavy metals, polycyclic aromatic hydrocarbons and combined pollution of the heavy metals and the polycyclic aromatic hydrocarbons; by promoting the growth of the sudangrass, improving the photosynthesis of the sudangrass, relieving the oxidation pressure of the sudangrass and changing the composition of soil microbial communities, the toxicity of chromium to the sudangrass is relieved, and the method has practical popularization and application values.
Owner:HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY +1

Phosphorus-dissolving bacterial agent for degrading hydroxyapatite, preparation method thereof, bio-bacterial fertilizer and application

The present application relates to the field of environment, the utilization of phosphate rock resources and the field of agricultural biotechnology, and in particular to a kind of hydroxyapatite-degrading phosphate-solubilizing bacterial agent and its preparation method, biological bacterial fertilizer and application, the hydroxyapatite-degrading phosphate-solubilizing bacterial agent includes phosphate-solubilizing bacteria, hydroxyapatite and culture medium, the phosphate-solubilizing bacteria is Pantoea agglomerate CT3.By using hydroxyapatite to prepare the hydroxyapatite-degrading phosphate-solubilizing bacterial agent, the full use of phosphate rock resources can be greatly promoted, and the dissolution of insoluble phosphorus in phosphate rock is realized.The hydroxyapatite-degrading phosphate-solubilizing bacterial agent combines Pantoea agglomerate CT3 with hydroxyapatite, which can utilize the phosphate-solubilizing ability of Pantoea agglomerate CT3 and fully utilize the phosphorus resources of hydroxyapatite.The hydroxyapatite-degrading phosphate-solubilizing bacterial agent has good prospects in the preparation of biological bacterial fertilizer, application in agricultural soil improvement, application in phosphate rock resource recovery and application in promoting wheat growth.
Owner:TIANJIN UNIVERSITY OF TECHNOLOGY +1

A strain with growth-promoting and salt-tolerant function derived from mangrove ecosystem and its application

The present invention discloses a strain with growth-promoting and salt-tolerant function derived from a mangrove ecosystem and its application. The strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 13, 2022, with a taxonomic name of Pantoeastewartii and a deposit number of GDMCC No: 62620; the strain can fix nitrogen, dissolve potassium, dissolve phosphorus, promote plant growth, and improve the relief of plant salt stress; compared with the reported strains, it can produce volatile organic compounds with growth-promoting functions under salt treatment, which can be used to prepare plant growth promoters, microbial fertilizers, soil conditioners, etc.; it has good application prospects.
Owner:SUN YAT SEN UNIV

Application of spirodela polyrhiza and microorganism combination in remediation of cadmium-polluted water and reduction of cadmium content of rice grains in cadmium-polluted rice field

The invention relates to application of spirodela polyrhiza and microorganism combination in repairing a cadmium-polluted water body and reducing the cadmium content of rice grains in a cadmium-polluted rice field. The preparation method comprises the following specific steps: firstly, respectively preparing a bacillus bacterium suspension, an enterobacter bacterium suspension and a pantoea bacterium suspension; mixing the three bacterial suspensions in proportion to obtain a mixed bacterial suspension; then the mixed bacterial suspension and spirodela polyrhiza are prepared according to the proportion and then added into a cadmium-polluted water body to be remedied or a cadmium-polluted rice field to be remedied, and spirodela polyrhiza and microorganisms form a spirodela polyrhiza-functional flora symbiotic system; the microorganisms convey the cadmium to the leaves through adsorption, leaf surface sedimentation conversion or transpiration; and finally, the spirodela polyrhiza is treated in a centralized manner, so that the cadmium-polluted water body is repaired or the cadmium content of rice grains is reduced. By means of the method, the cadmium-polluted water body can be well repaired, and the cadmium content of rice grains in the cadmium-polluted rice field can be effectively reduced.
Owner:CHENGDU UNIV

Composition containing acetobacter saxatilis and nitrogen-fixing pantoea and application of composition

The invention provides a composition containing acetobacter saxatilis and pantoea azototrophicus and application of the composition. The composition provided by the invention comprises acetobacter sativus or progeny thereof and pantoea azototrichum or progeny thereof; the registration number of the acetobacter breviscapus in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36149; the registration number of the nitrogen-fixing pantoea in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36150. The invention further discloses a preparation method of the nitrogen-fixing pantoea. The acetobacter sativus and the nitrogen-fixing pantoea or the composition of the acetobacter sativus and the nitrogen-fixing pantoea can be used for reducing emission of nitrogen-containing gas in the livestock breeding process and the livestock manure (normal state and composting) treatment process, and has a good application prospect.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS +1

Biocontrol pandoraea KBA33 inhibiting botryosphaeria dothidea and application thereof

ActiveCN119859590BBiocideBacteriaBiotechnologyEnterobacter agglomerans
The application discloses a biocontrol pantoea agglomerans KBA33 for inhibiting botryosphaeria dothidea and application thereof. The strain KBA33 disclosed by the application can effectively inhibit botryosphaeria dothidea at 16 DEG C and 28 DEG C, and can inhibit botryosphaeria dothidea through cell-cell contact.
Owner:NANJING AGRICULTURAL UNIVERSITY

Pantoea agglomerans with high glutamic acid yield, method and application

The invention belongs to the technical field of microorganisms, and discloses a Pantoea agglomerans strain with high glutamic acid yield, the name of the Pantoea agglomerans strain is PA-GLU-10, the classification name is Pantoea agglomerans, the preservation number is GDMCC 65358, the preservation date is October 28, 2024, and the preservation unit is Guangdong Microbial Culture Collection Center, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The invention provides Pantoea agglomerans, the Pantoea agglomerans can be used for fermentation production of glutamic acid, has the advantages of tolerance to relatively low pH in a fermentation process, high glutamic acid production speed, suitability for continuous culture and the like, overcomes the problems of poor acid resistance, high cost and the like of an existing fermentation strain, and is expected to reduce the production cost of glutamic acid.
Owner:COFCO BIO CHEM ENERGY LONGJIANG CO LTD +1

Preparation method and application of radix scrophulariae sweating processed product

The invention discloses a preparation method and application of a radix scrophulariae sweating processed product. The method comprises the following steps: raw material treatment: selecting fresh radix scrophulariae, and removing non-medicinal parts; processing: regulating and controlling microbial communities in radix scrophulariae in a manner of combining steaming, drying and sweating; the method specifically comprises any one of direct drying at 60 DEG C, repeated sweating for 3 days to dry after drying at 60 DEG C to half-dry, direct repeated sweating for 3 days to dry, direct drying at 60 DEG C after steaming, direct drying at 60 DEG C after steaming to half-dry and repeated sweating for 3 days to dry, and direct repeated sweating for 3 days to dry at 60 DEG C after steaming. According to the method, the abundance change of key microorganisms such as pseudomonas aeruginosa, bacillus, pantoea and cladosporium is directionally promoted through a specific processing technology, and a new way is provided for quality standardization and quality improvement of radix scrophulariae processed products.
Owner:CHONGQING DRUG PLANTING RES INST

Peach leaf essential oil and application thereof

The invention relates to the technical field of essential oil, in particular to peach leaf essential oil and application thereof. According to the method, the peach leaf essential oil is extracted by adopting a water distillation method, the components of the peach leaf essential oil are identified, 40 volatile chemical components are identified by utilizing headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS), 23 chemical components are identified by utilizing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), and the content of the volatile chemical components in the peach leaf essential oil is greatly improved. Researches find that the peach leaf essential oil can effectively inhibit and kill escherichia coli, staphylococcus aureus, micrococcus luteus, pantoea, fusarium oxysporum and monilinia fusarium, and in addition, the invention also finds that the peach leaf essential oil has better oxidation resistance and free radical scavenging ability, so that the invention develops new application of the peach leaf essential oil.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Selenium-enriched biological fertilizer capable of promoting plant growth and preparation method of selenium-enriched biological fertilizer

ActiveCN121574850ABio-organic fraction processingBacteriaEnterobacter agglomeransMicroorganism
The invention provides a bio-fertilizer for promoting plant growth and enriching selenium and a preparation method thereof, and belongs to the technical field of bio-fertilizers, the bio-fertilizer is based on a dried and sterilized mixed organic matter and a direct vat set pantoea inoculant, the mass fraction of N in the mixed organic matter is 4-8%, the mass fraction of P2O5 in the mixed organic matter is 1.6-3.3%, and the mass fraction of K2O in the mixed organic matter is 1.6-3.3%. The selenium-enriched biological fertilizer for promoting plant growth is characterized in that the selenium-enriched biological fertilizer for promoting plant growth is prepared by mixing and fermenting mixed organic matters, calcium superphosphate, sodium selenite and a direct-vat-set pantoea agglomerans fungicide, the pantoea agglomerans AKFX-001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122. The strain disclosed by the invention has the effects of resisting high-concentration selenium, converting inorganic selenium into organic selenium and promoting plant growth at the same time, and a single strain realizes the functions which can be realized by a previous mixed strain, so that the strain has a very good market prospect.
Owner:ANKANG SELENIUM-ENRICHED PROD R&D CENT

Pantoea BG4-1 with growth promoting effect and application thereof

The invention relates to the technical field of microorganisms, in particular to pantoea BG4-1 with a growth promoting effect and application of the pantoea BG4-1. The pantoea BG4-1 is preserved in the China General Microbiological Culture Collection Center on June 20, 2025, and the preservation number of the pantoea BG4-1 is CGMCC NO.34960. The pantoea BG4-1 has relatively strong capabilities of dissolving phosphorus, dissolving potassium and producing IAA (Indoleacetic Acid), and has a remarkable growth promoting effect on capsicum and arabidopsis thaliana. According to the invention, a high-quality strain resource is provided for developing a novel efficient biological phosphorus-potassium fertilizer, the method has important meanings for improving the utilization rate of phosphorus and potassium elements in soil, reducing the application of chemical phosphorus-potassium fertilizers and promoting the sustainable development of agriculture, meanwhile, a new way is provided for resource utilization of invasive plants, and the ecological management concept of'taking harm as benefit 'is favorably realized.
Owner:GUIZHOU AGRI VOCATIONAL COLLEGE

Pantoea H1 as well as demonstration method and application thereof in agriculture assistance

The invention relates to the technical field of microorganisms, and discloses pantoea H1 and a demonstration method and application thereof in agriculture assistance, the pantoea H1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36875. The invention further provides a microbial agent containing the bacterial strain, a preparation method and application of the bacterial strain in crop planting, the bacterial strain has efficient dual phosphorus solubilizing capacity, indissolvable inorganic phosphorus can be dissolved, organophosphorus can be mineralized, and the effectiveness of soil phosphorus is improved; meanwhile, the microbial agent has excellent saline-alkaline tolerance, can still keep high activity under adversity, and by applying the microbial agent in the seedling stage of crops, the rhizosphere microenvironment can be remarkably improved, root development and plant growth of the crops such as corn and mung beans can be promoted, the emergence rate and yield of the crops in soil such as saline-alkaline land can be increased, and the crop yield can be increased. And an excellent microbial germplasm resource is provided for activating soil nutrients, reducing weight and improving efficiency.
Owner:YULIN UNIV

Phosphate-solubilizing bacteria, their preparation methods and applications

This invention belongs to the field of microbial technology, and specifically relates to a highly efficient phosphate-solubilizing bacterium, its preparation method, and its application. The phosphate-solubilizing bacterium is named Pantoea cypripediP11, with the biological preservation number CCTCC NO: M2023159. The highly efficient phosphate-solubilizing bacterium Pantoea cypripediP11 proposed in this invention can convert insoluble phosphorus into soluble phosphorus at a maximum content of 663.59 mg / L, which can increase the available phosphorus content in the soil by 1.46 times. This is stronger than the phosphate-solubilizing ability of the existing phosphate-solubilizing bacterium Burkholderia ubonensis, and it can also produce indoleacetic acid and secrete siderophores to promote plant root growth.
Owner:ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Application of Pantoea ananatis

The invention discloses an application of Pantoea ananatis, and relates to the technical field of microorganisms. Aiming at the defects of the existing obesity intervention means, the invention provides a brand-new solution: Pantoea anatitis adapted to a high-altitude environment is used as a core functional strain, through an intestinal flora driving mechanism, the expression of UCP1 protein in brown adipose tissue (BAT) is activated, the heat production function of the BAT is enhanced, and meanwhile, the storage of white adipose tissue (WAT) and the level of liver triglyceride (TG) are reduced; the weight-losing effect is realized. The strain can play a role through an'intestinal flora-BAT 'axis without cold exposure, has no obvious influence on liver functions, and is excellent in safety. The invention discloses a flora-mediated non-cold-dependent BAT activation way for the first time, can be used for preparing products such as weight-reducing medicines and health-care foods, has the advantages of mechanism innovation, exact effect and wide application, and provides a new technical choice for obesity intervention.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI

Phage CM23S1 with function of splitting pantoea agglomerans and application of phage CM23S1

PendingCN121538186ABiocideMicroorganism based processesEnterobacter agglomeransMicroorganism
The invention provides a bacteriophage CM23S1 with a function of splitting pantoea agglomerans and application of the bacteriophage CM23S1, and belongs to the technical field of biological control. The phage is named as CM23S1, the whole length of a genome sequence is 92308 bp, the phage is preserved in the China General Microbiological Culture Collection Center on October 21, 2025, and the preservation number is CGMCC No.46684; the invention provides a method for separating and purifying the bacteriophage and preparing a biological preparation. The bacteriophage has relatively high stability and splitting property on pantoea agglomerans. An indoor bacteriostasis test shows that the bacteriophage has an obvious bacteriostasis effect on pantoea agglomerans, and a potted wheat test also proves that the bacteriophage can be used for preventing and treating plant diseases caused by pantoea agglomerans.
Owner:SHANDONG PUYUAN BIOLOGICAL ENG CO LTD

Brevibacterium autochromatum and method for decarburizing and denitrifying wastewater under anaerobic condition by using brevibacterium autochromatum

The invention discloses a brevibacterium anodorum strain and a method for carbon and nitrogen removal of wastewater under anaerobic conditions, and belongs to the field of biological treatment of water body pollution. The brevibacterium pigmentum JLG5-316-4 is obtained through separation, the brevibacterium pigmentum has the capacity of efficiently degrading nitrogen and carbon in wastewater under the anaerobic condition, the pantoea agglomerans provided by the invention can have high denitrification capacity under the environments that the pH value is 7.5, the temperature is 30 DEG C, the illumination condition is 5000lux and the like, and it can be seen that the pantoea agglomerans can be used for efficiently degrading nitrogen and carbon in wastewater under the conditions that the pH value is 7.5, the temperature is 30 DEG C, the illumination condition is 5000lux and the like. The brevibacterium edulis provided by the invention can be used for improving the water quality in an anoxic environment.
Owner:JIANGSU UNIV OF TECH +1

Pantoea widely distributed strain YC2472 and application thereof

ActiveCN121160573ABiocidePlant growth regulatorsPantoea vagansMicroorganism
The invention provides a pantoea widely distributed strain YC2472 and application thereof, and belongs to the technical field of biological control. The taxonomic name of the YC2472 strain is Pantoea vagans, and the YC2472 strain is preserved in Guangdong Microbiological Culture Collection Center, and the address is Institute of Microbiology, Guangdong Academy of Sciences, Floor 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou; the preservation date is July 4, 2024; the preservation number is GDMCC No: 64827. According to the invention, a bacterial strain YC2472 with stable and significant antagonistic activity on the pathogenic bacteria is separated and screened from rhizosphere soil of tobacco, and the bacterial strain YC2472 is identified as Pantoea vulgaris. Retrieval of domestic and foreign literatures shows that the biocontrol activity of Pan.vans on phytophthora nicotianae is not reported, so that the innovativeness and potential application value of the Pan.vans are highlighted.
Owner:GUIZHOU TOBACCO SCI RES INST

Complex microbial inoculant for onions and preparation method of complex microbial inoculant

The invention discloses a complex microbial inoculant for onions and a preparation method of the complex microbial inoculant. The complex microbial inoculant comprises acinetobacter JQ-MY-41 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.32731, pantoea JQ-MY-42 with the preservation number of CGMCC NO.32732 and alcaligenes YC-342 with the preservation number of CGMCC NO.32733, and the optimal mass ratio of the acinetobacter JQ-MY-41 to the pantoea JQ-MY-42 to the alcaligenes YC-342 to the alcaligenes YC-342 is 30: 25: 30. The preparation method comprises the steps of strain activation, strain culture, thallus collection, strain compounding, carrier addition, drying and the like. The three strains achieve synergistic growth promotion through different mechanisms such as phosphorus solubilization, nitrogen fixation and IAA production, and compared with a control group, the onion plant height is increased by 37.5%, the stem diameter is increased by 39.0%, the total biomass is increased by 103.7%, and the total chlorophyll amount is increased by 69.5%; the problems that an existing microbial agent is single in function and limited in growth promoting effect are solved, and technical support is provided for green and efficient cultivation of onions.
Owner:VEGETABLE RES INST OF GANSU ACAD OF AGRI SCI

Real-time fluorescent RT-qPCR detection method for viable bacteria of maize bacterial wilt germs

The invention relates to the technical field of agricultural biology, and discloses a real-time fluorescent RT-qPCR detection method for viable bacteria of maize bacterial wilt germs, which comprises the following steps: S1, screening key genes; s2, designing a primer and a probe of a key gene; s3, evaluating the stability of the key gene mRNA after inactivation, and determining a target gene; the target gene is a LexA gene with a nucleotide sequence as shown in SEQ ID NO: 1; and S4, establishing a real-time fluorescent RT-qPCR detection method by adopting the primer and the probe corresponding to the target gene screened in the step S3. The efficient and accurate real-time fluorescent RT-qPCR detection method established by the invention has good specificity and sensitivity, and the sensitivity of detecting the mRNA of the PSS LexA gene is 1000 times that of a conventional RT-PCR method; the method can be used for quickly judging the condition that the PSS live bacteria are carried in an actual sample, and can provide powerful technical support for quarantine and prevention and control of PSS epidemic situations.
Owner:TECH CENT OF GUANGZHOU CUSTOMS

Bio-control bacterium for bighead atractylodes rhizome root rot and application thereof

PendingCN121699803ABiocideBacteriaBiotechnologyRhizobacteria
The invention discloses an atractylodes macrocephala root rot biocontrol bacterium and application thereof, and belongs to the technical field of plant disease biocontrol. The rhizoma atractylodis macrocephalae rhizosphere bacterium is separated from healthy rhizosphere soil of a compositae plant rhizoma atractylodis macrocephalae by adopting a rhizosphere bacterium separation and purification technology, is classified and named as Pantoea rodsii RSB15, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M20251954, and the preservation date is September 1, 2025. The invention also discloses application of the bacterium: the bacterium can effectively inhibit the growth of pathogenic bacteria of bighead atractylodes rhizome root rot, and the inhibition rate of plate confrontation culture reaches 20.57%; the morphological structure of pathogenic fungi can be destroyed. The bacterium provided by the invention has an obvious effect of preventing and treating bighead atractylodes rhizome root rot and provides a wide application prospect in the field of biological prevention and treatment of bighead atractylodes rhizome fungal diseases.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY

Pseudomonas orientalis LJ-6 and application thereof in prevention and control of mulberry diseases

ActiveCN120118770ABiocideBacteriaBiotechnologyColletotrichum gossypii
The invention discloses a strain of extreme oriental pseudomonas LJ-6 and application thereof in prevention and control of mulberry diseases. The extreme oriental pseudomonas disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number of the extreme oriental pseudomonas is GDMCC (China General Microbiological Culture Collection Center) NO: 64766. The extreme pseudomonas orientalis LJ-6 has a very good antagonistic effect on bacterial pathogenic bacteria such as lauraceae bacteria, pantoea anantoea, Klebsiella compacta and enterobacter cloacae and fungal pathogenic bacteria such as fusarium oxysporum, curvularia and colletotrichum truncatum, and has a very good antagonistic effect on plant diseases caused by plant infection by the pathogenic bacteria. The composition has good prevention and control effects on mulberry bacterial wilt, mulberry bacterial wilt, mulberry leaf spot, mulberry anthracnose and the like, and is safe and effective. Therefore, the extreme oriental pseudomonas LJ-6 disclosed by the invention has good application prospect and value in the aspects of preventing and controlling plant diseases such as mulberries and preparing disease prevention and control preparation products.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Salt-tolerant endogenous pantoea and application thereof

The invention discloses salt-tolerant endogenous pantoea and application thereof, and relates to the technical field of microorganisms. According to the invention, a strain of endophytic pantoea TH1 is separated from young grape fruits, and the preservation number of the endophytic pantoea TH1 is CCTCC (China Center For Type Culture Collection) NO: M 2025960. The strain can promote the growth of grapes, improve the quality of the grapes, increase the content of vanillin in the grapes, increase the content of anthocyanin, flavonoid and flavanone in the grapes and reduce the content of tannin and total phenols in the grapes. Besides, the strain has the capabilities of efficiently dissolving potassium, fixing nitrogen, dissolving phosphorus and producing IAA (indoleacetic acid) and salt resistance, can improve plant photosynthesis, improve soil environment and promote plant growth under the condition of salt stress, and can also be used for preventing and treating grape powdery mildew at the same time.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Corn endophytic disperse pantoea X24004 and application thereof

The invention discloses corn endophytic pantoea dispersa X24004 and an application of the corn endophytic pantoea dispersa X24004. The preservation number of the Pantoea dispersa X24004 is CGMCC (China General Microbiological Culture Collection Center) No. 34510, and the preservation number of the Pantoea dispersa X24004 is CGMCC No. 34510. The pantoea dispersa X24004 has a relatively strong antagonistic effect on a plurality of pathogenic fungi such as fusarium graminearum, fusarium verticillioides, pythium aphanidermatum stem rot pathogenic bacteria and the like, has high efficiency and broad spectrum on prevention and treatment of plant fungal diseases, and also has a promoting capability on growth of corn plants; the biological agent is a broad-spectrum biological agent, a biological bacterial fertilizer and a seed treatment agent with good development and application prospects.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI