Microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone

A technology of microbial transformation and ionone, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of improving tobacco quality, huge application prospects, and efficient degradation

Inactive Publication Date: 2015-12-16
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, there is no literature report that it can degrade lutein to produce 3-hydroxy β-ionone and β-ionone

Method used

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  • Microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone
  • Microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone
  • Microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone

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Embodiment Construction

[0016] A kind of microbial conversion synthetic 3-hydroxy β-ionone and β-ionone method, its steps are as follows:

[0017] (1) Pantoea disperse was stored in ultra-low temperature at -80°C, and the protective agent was 20% glycerol; for Pantoea disperse culture, the Pantoea disperse strain was inoculated into the triangular flask containing the seed medium, and the formula of the seed medium was: Distilled water 1L, sucrose 10g, asparagine 4.5g, K 2 HPO 4 1.5g, MgSO 4 0.5g, yeast extract 3.0g, FeSO 4 0.01g, under the condition of pH=6.5, 30-34℃, shake culture at 180-200r / min for 10-18h to obtain seed liquid;

[0018] (2) Seed tank fermentation: Inoculate the seed liquid with an inoculation amount of 1% by volume into a seed tank equipped with a seed tank liquid fermentation medium for fermentation, until the bacteria enter the logarithmic growth phase, and the seed tank liquid fermentation The medium is: based on the quality of 1L of water, the following ingredients are ad...

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Abstract

The invention discloses a microbial-conversion-based method for synthesizing 3-hydroxy beta-ionone and beta-ionone. The method includes the following steps: (1), culturing scattered pantoea, inoculating a triangular bottle filled with a seed culture medium with a scattered pantoea strain, and oscillating at 180-200r/min under the conditions of pH being 6.5 and temperature being 30-34 DEG C for culture for 10-18h to obtain seed liquid; (2), fermenting in a seed tank; (3), obtaining 3-hydroxy beta-ionone and beta-ionone. By searching fermentation culture media, an optimal fermentation culture medium capable of efficiently degrading lutein is obtained, and 3-hydroxy beta-ionone and beta-ionone which have aromatic substances can be generated. The 3-hydroxy beta-ionone and beta-ionone are important aromatic substances, and tobacco quality can be improved greatly by adding a small amount of the aromatic substances into tobacco. The microbial-conversion-based method has huge application prospect in utilizing biologically-degraded lutein and generating natural aromatic substances.

Description

technical field [0001] The invention belongs to the field of biotransformation, uses dispersed pantoea to degrade lutein to generate aroma substances 3-hydroxyl β-ionone and β-ionone, and relates to the optimization and application of the transformation medium of pantoea microorganisms. Background technique [0002] Lutein is widely found in flowers, fruits and vegetables, tobacco and other plants, and is the precursor of many important aroma components. During the ripening and burning process of flue-cured tobacco, lutein can be degraded and transformed into β-ionone, macrostigmatrienone, etc., which have strong floral and fruity aromas and low aroma thresholds, and are of great value in the preparation of flavors and fragrances . However, the degradation of lutein is currently mainly focused on physical and chemical degradation, which lacks specificity. There are few studies on biodegradation methods, especially the use of enzymes and microorganisms to degrade lutein to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/01
Inventor 杨雪鹏叶建斌毛多斌赵越张展马科胡仙妹邵化
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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