29results about How to "Good yield" patented technology

The present application relates to the technical field of resource utilization of fruit tree branches, and provides a method for returning citrus branches to the orchard, which comprises the following steps: (1) crushing and fermenting the pruned citrus branches, and mixing the branches with organic fertilizer to obtain material A; (2) digging a ditch along a direction parallel to the tangent of the tree crown drip line; and (3) applying the material A obtained in the step (2) into the ditch, and then covering the ditch with soil. The method for returning citrus branches to the orchard can significantly improve the diversity of arbuscular mycorrhizal fungi and the stability of the community structure in the soil, and promote more arbuscular mycorrhizal fungi to act on the rhizosphere soil and root hairs of citrus, so as to effectively improve the physicochemical properties of the soil and promote the growth and development of citrus. The method for returning citrus branches to the orchard is a resource utilization of waste, and can better promote the green, low-carbon, efficient and sustainable development of the citrus industry.

The utility model relates to the technical field of agilawood formation, and discloses an ozone agilawood formation promoting device which comprises a control box, the top of the control box is fixedly connected with an ozone generator, the output end of the ozone generator is fixedly connected with an output pipe, and one end of the output pipe is fixedly communicated with a pipeline branching connector. And one output end of the pipeline branching connector is fixedly communicated with an air pipe. Through cooperative use of the ozone generator, the output pipe, the pipeline branching connector, the air pipe, the ozone conveying pipe, the storage battery, the photovoltaic charging and discharging management module, the time controller and other structures, ozone can be continuously provided for the interior of the aquilaria sinensis tree, so that the interior of the aquilaria sinensis tree is highly oxidized, aquilaria sinensis is promoted to form agilawood, and the aquilaria sinensis tree is protected from being damaged. The agilawood production device solves the problem of low yield of agilawood production through punching, avoids fire hazards caused by open fire in traditional methods such as burning of iron nails, and improves the practicability of the agilawood production device.

The invention relates to the technical field of fruit and vegetable cultivation, and provides a water chestnut fertilization influence analysis method based on principal component analysis, which comprises the following steps: setting a plurality of field trials for different nitrogen, phosphorus and potassium fertilization level treatment; measuring plant growth indexes, yield indexes, corm quality indexes and soil physicochemical property indexes of the treated water chestnuts; performing principal component analysis on the measured multiple indexes, and extracting principal components of which the characteristic values are greater than 1; calculating comprehensive scores of the processing according to the scores of the principal components and sorting the comprehensive scores; according to the comprehensive score, evaluating the comprehensive influence of different fertilization treatments on the growth, yield, quality and soil properties of the water chestnuts, and screening out an optimal fertilization combination. According to the method, the matching of medium-low nitrogen and phosphorus and medium-high potassium is clear, so that high yield and high quality are guaranteed, soil acidification can be effectively relieved, excessive consumption of nutrients is avoided, synergism of fertilizer saving, efficiency improvement, soil health and quality improvement is realized, and data support and quantitative decision basis are provided for scientific fertilization of the water chestnuts.

The application discloses a single-core fruiting Pleurotus eryngii strain and a cultivation method, and belongs to the technical field of biology.The application first separates the Pleurotus eryngii strain ZNS001 from a single spore of the Pleurotus eryngii, and the preservation number is CGMCC No.42480.The single-core mycelium of the Pleurotus eryngii strain ZNS001 is solid and grows into a fruiting body, and through agronomic trait determination, it is found that the cap of the Pleurotus eryngii strain is trumpet-shaped, and the diameter is 1.9-4.4 cm; the stipe is relatively short, the stipe length is 0.8-3.2 cm, and the stipe thickness is 0.5-3.9 cm.The Pleurotus eryngii strain of the application provides a material for studying the sexual reproduction and development process of fungi, and helps to deeply understand the biological processes such as fungal cell differentiation and morphological formation, so that hybrid breeding can be more effectively carried out.

The invention belongs to the field of plant genetic engineering, particularly relates to a rice long-grain gene GL1 and application thereof, and aims to solve the current situation of imbalance of high yield and high quality and synergistically improve the rice yield and quality so as to ensure grain safety. The invention provides a rice grain length and weight regulation gene GL1. The nucleotide sequence of the gene GL1 is as shown in SEQ ID NO: 1. The CDNA sequence of the gene GL1 is as shown in SEQ ID NO: 2. The rice grain length and weight regulating gene GL1 can be used for increasing the length and weight of rice grains and increasing the length, length-width ratio and weight of the rice grains, so that the yield and appearance quality of the rice are improved.

The invention provides a method for growing and stripping polycrystalline diamond with low stress, the principle of the method is that the characteristic that a substrate and a transition layer are low in mismatch with the polycrystalline diamond is utilized, and meanwhile, the substrate is etched by hydrogen after the polycrystalline diamond grows by utilizing the strong etching capability of the hydrogen, so that the intervention of extra stress is avoided, and the polycrystalline fracture is reduced. Compared with the prior art, the scheme can effectively reduce the breakage of the polycrystalline diamond during stripping, and can be applied to solve the problem of low yield caused by breakage of the industrial MPCVD polycrystalline diamond.

The present application relates to the technical fields of microbial genetic engineering and fermentation engineering, and particularly relates to a recombinant Ogataea phaffii engineering bacterium and a construction method and application thereof.The present application integrates cns1, cns2 and cns3-NK into the Kp-Con genome, and constructs a multi-copy strain capable of efficiently synthesizing cordycepin.Furthermore, the als3 gene or the gcw16Δcbd gene is integrated into the genome of the cordycepin-producing multi-copy strain, thereby enhancing the biofilm-forming ability of the recombinant strain.Combined with the optimization of fermentation medium and conditions, the cordycepin yield of the recombinant strain can reach 6.41 g / L under free fermentation conditions.Through the combination of immobilized continuous fermentation strategy, the overall average cordycepin yield of the recombinant strains KpCor-A441-als3 and KpCor-A441-gcw16Δcbd is increased by 25.4% and 19.3% respectively compared with the control strain.

The application discloses a lignin carbon-based halogen bismuth oxide Z-type heterojunction composite material with regular flower-like morphology and a preparation method and application thereof. A lignin carbon-based halogen bismuth oxide Z-type heterojunction composite material BiOX / C with regular flower-like morphology, wide spectral response range, high efficiency and strong stability is synthesized by using lignin carbon with rich oxygen-containing functional groups for induction and pH regulation. Based on the flower-like morphology structure and Z-type heterojunction of the material, the separation of photo-generated carriers and holes is effectively promoted, so that the material has higher photo-reaction efficiency, and the shortcomings of traditional photocatalytic degradation of organic pollutants, such as low quantum efficiency, slow degradation rate, poor stability, easy deactivation and the like, are effectively solved. Moreover, the lignin carbon-based substrate material takes lignocellulose in agricultural waste as a carbon source, is environment-friendly and low in production cost, and has the advantages of simple synthesis method, considerable yield, mild reaction condition in the process of photocatalytic degradation of organic pollutants, simple operation and wide application prospect.

The present application belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered strain with high yield of acetoin and 2,3-butanediol, a construction method and application. The present application obtains acoR deletion mutant MW03-ΔA by knocking out the regulatory gene acoR of the acetoin dehydrogenase enzyme system, blocks the degradation of AoDHES to acetoin, effectively reduces the production of by-products, thereby facilitating the accumulation of more acetoin. A strong promoter expression vector is further constructed in the MW03-ΔA strain, and the alpha-acetyl lactate decarboxylase gene alsD is connected, and then introduced into the acoR deletion mutant MW03-ΔA of Bacillus licheniformis, so as to overexpress alpha-acetyl lactate decarboxylase, and obtain a genetically engineered strain MW-BL4 with high yield of acetoin and 2,3-butanediol. The present application has the beneficial effect that compared with a single product of the engineered strain, the present application can realize the production of acetoin and 2,3-butanediol and other multiple products by using a single strain, and the yield is considerable, which can significantly reduce the cost of bacterial strains in industrial production and improve the production efficiency.

The invention discloses a resource utilization method of electric furnace steelmaking synergistic antibiotic dregs, which utilizes a foam slag operation process in an electric furnace steelmaking production process to synergistically process the antibiotic dregs, and after the antibiotic dregs are granulated, the granulated antibiotic dregs are used as a foaming agent of electric furnace steelmaking foam slag for resource utilization. In the electric furnace steelmaking process, high temperature of electric furnace steelmaking is used for inactivating the antibiotic dregs, harmless conversion of heavy metal in the antibiotic dregs is completed through reduction reaction in the electric furnace foam slag generation process, and a traditional carbon foaming agent in the electric furnace steelmaking process can be replaced. The use amount of the foaming agent carbon powder of the foaming slag in the electric furnace steelmaking process is saved, and the antibiotic medicine residues can be treated on a large scale.

The application discloses a novel polyhydroxyalkanoate (PHA) polymerase (PhaC) which can efficiently polymerize the activated monomer 6-hydroxyhexanoate-CoA (6HHx-CoA) of 6-hydroxyhexanoate (6HHx). The PhaC is co-expressed with a CoA ligase AlkK, and can synthesize poly-epsilon-caprolactone (PCL) from the direct precursor sodium 6-hydroxyhexanoate in a halomonas sp. Halomonas which has deleted endogenous beta-ketothiolase (PhaA), acetoacetyl-CoA reductase (PhaB), PhaC and enoyl-CoA hydratase (FadB).

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

The invention provides a method for preventing and controlling tomato bemisia tabaci by using nesidiocoris tenuis, and relates to the technical field of pest prevention and control. The method comprises the following steps: S1, raising seedlings of tomatoes, and transplanting 20-25 days after raising the seedlings; s2, according to an intercropping mode that two rows of kidney bean planting belts are arranged every 3-4 rows of tomatoes, the kidney beans are arranged in a staggered mode and planted in different periods; s3, after tomato seedlings are planted, the density of the nesidiocoris tenuis in the kidney bean planting zone and the density of bemisia tabaci on tomato plants are monitored in real time, and the nesidiocoris tenuis is supplemented according to the monitored value; s4, performing field management. According to the method, the kidney beans and the tomatoes are intercropped, the kidney beans are used for providing a good survival and inhabitation environment for the nesidiocoris tenuis, good disease and pest prevention and control can be achieved in the whole growth period of the tomatoes, meanwhile, the yield of the tomatoes and the kidney beans can be improved, and economic benefits are increased.

The application discloses a kind of perennial wild peanuts as grass fire dragon fruit cultivation method, S1.selects ground, ground and ridge;S2.punching post, planting pitaya seedling;S3.transplanting wild peanuts: wild peanuts branch according to travel distance 20~25cm, plant distance 15~20cm in the strip ditch of pitaya garden;S4.water and fertilizer management;S5.mowing: when wild peanuts flower basically withers, leaf color presents old green as a whole, that is, the aboveground part of wild peanuts is mowed with mower and directly returned to field, underground part rhizome is retained and respectively in March, June, September and November every year, a total of 4 times mowing.The application adopts perennial rhizome area group wild peanuts A.glabrata (PI262801) intercropping cultivation pitaya, and the pitaya garden benefits for many years after grafting planting once, can inhibit weed growth, compared with other green manure, wild peanuts have good effect on deep soil improvement, and the effect is remarkable.

A method for growing plants, including a planter and a harvester, both of which include separating seeds and measuring parameters of the seeds as they are separated. This can be used to plant selected seeds and harvest specific plants. The system operates by correlating information from seeds planted in individual plantings and harvested seeds with respect to specific locations on a growth medium, and the system can include information about the growth medium at the locations. The locations can be determined by plants in a planting pattern, where the pattern can be detected by a reader on the harvester. The system can be used to control selection of seeds to take into account soil conditions for the plants.

This invention discloses a combined power cycle system and method for cooling incoming air using ammonia-water, relating to the aerospace field. The system can switch between turbine-ramjet operating modes through an adjustable structure. Particularly during turbine-ramjet switching, ammonia-water is used to cool the high-temperature incoming air in the intake duct. The absorbed ammonia-water is then distilled to separate high-temperature ammonia gas and water. This invention combines heat exchanger precooling and jet precooling, effectively increasing the pressure ratio of the fan and compressor through multiple air cooling processes. This improves the compressor outlet gas temperature, thereby reducing compressor power consumption and providing greater thrust. Ammonia, as a supplementary fuel, has a higher specific impulse, addressing the thrust gap during engine mode transitions and effectively widening the operating speed range of the turbine engine.

A blown film die head temperature control structure includes a blown film die head with an annular extrusion port. The blown film die head is evenly divided into N sector-shaped regions along its circumference, where N is a natural number. Each sector-shaped region is equipped with two arc-shaped heat-conducting oil pipes, the center of each heat-conducting oil pipe located on the vertical central axis of the blown film die head. The two heat-conducting oil pipes are close together, each extending from a first circumferential end to a second circumferential end of the corresponding sector-shaped region. Each heat-conducting oil pipe has an inlet and an outlet. In each sector-shaped region, one heat-conducting oil pipe has its inlet on the clockwise side and its outlet on the counterclockwise side, while the other heat-conducting oil pipe has its inlet on the counterclockwise side and its outlet on the clockwise side. This invention can maintain a basically uniform temperature at various points along the circumference of the blown film die head and can quickly adjust and change the temperature.

The invention belongs to the technical field of gene engineering, and discloses an application of a PagPUB9 gene in regulation and control of poplar growth and development, the nucleotide sequence of the PagPUB9 gene is shown as SEQ ID NO.1, and through overexpression of the PagPUB9 gene, poplar growth and development are promoted. The invention provides the application of the PagPUB9 gene in regulating and controlling the growth and development of the poplar, the growth and development speed of the poplar can be promoted through overexpression of the PagPUB9 gene, xylem differentiation and stem thickening are promoted while a poplar plant becomes high, and therefore a new gene resource and a new molecular breeding means are provided for genetic improvement of the poplar.

This invention discloses a high-glutathione-producing engineered Escherichia coli strain and its applications, belonging to the field of biotechnology; the engineered Escherichia coli strain is named ZMGSH01F, with accession number CCTCC M 20252236; the engineered Escherichia coli strain is based on Escherichia coli BL21(DE3) as the starting strain, with gene clusters knocked out. ptsH‐ ptsI‐crr And integrate at this site glf‐glk Fusion genes, knockout gene clusters gsiA‐gsiB‐gsiC Integrate the endogenous glutathione transporter gene at this site. cycDC Knockout of γ-glutamyltransferase gene ggt Simultaneously expressing the recombinant plasmid pRSFDuet- gshAB K177E‑E275K‑V513A - ppk2 The engineered Escherichia coli strain can efficiently produce glutathione through fermentation culture using glucose, monosodium glutamate, cysteine, and glycine as substrates.

The invention discloses a process for synergistically treating cyanogen-containing hazardous waste by using high-temperature steel slag, which comprises the following steps: uniformly mixing the cyanogen-containing hazardous waste, a magnesium raw material and fly ash according to a mass percentage ratio of 50%: 30%: 20%, and producing pellets with the size of 30-50mm by using a dry powder pelletizer, the chemical components of the pellets are not required, and the water content is lower than 10%; when converter steel slag is poured out after converter smelting is finished, the pellets are added along with liquid steel slag, and the adding amount of the steel slag per ton is controlled to be 50-100 kg; after the pellets are added, the converter steel slag treatment process is not changed. High-temperature alkaline steel slag generated in the converter steelmaking production process is used for cooperatively treating the cyanogen-containing hazardous waste, harmful components in the cyanogen-containing hazardous waste are subjected to harmless conversion through the characteristics of high-temperature and alkaline process mineral structures in the converter steel slag treatment process, and meanwhile heavy metal in the cyanogen-containing waste slag is recycled to serve as raw materials for steelmaking for resource utilization. The hazardous waste treatment cost is reduced.

The application discloses a tylosin A high-yield engineering strain and application thereof, and belongs to the field of genetic engineering and fermentation technology. The strain is obtained by integrating a carbon mycose synthesis gene module (M14) overexpression cassette driven by a constitutive strong promoter into a genome and knocking out a negative regulation gene tylQ , so that the yield is greatly improved. Experiments show that the yield of the engineering strain in FFM culture medium reaches 1183 mg / L, which is 10 times that of a wild type; the yield can reach 1491.5 mg / L after adding alpha-ketoglutaric acid, and the genetic stability is good. The 5L fed-batch fermentation titer reaches 5.63 g / L. The application provides an efficient and stable industrial strain for large-scale production of tylosin A.

This invention belongs to the field of agricultural microbial technology, and discloses a strain of *Chlorella vulgaris* and its application in improving the stability of mushroom residue substrate. The *Chlorella vulgaris* strain is *Chlorella vulgaris* (…). Chryseobacterium The strain JYT19 (sp.) was deposited on February 2, 2026, with accession number CGMCC No. 37640. It is a new strain isolated from coconut coir material. Inoculating this strain into mushroom substrate significantly improves the physical structural stability of the substrate, delays collapse and compaction, and extends its service life. Simultaneously, it has beneficial effects on plant growth and quality improvement. This invention provides an environmentally friendly and efficient microbial technology solution to address the problems of rapid degradation and poor stability of mushroom substrate, and has significant application value in the fields of soilless cultivation in facility agriculture and resource utilization of organic waste.

The present invention relates to processes for the preparation of Tyk2 inhibitors. In particular, the present invention relates to improved processes for the synthesis of 6-(cyclopropanecarboxamido)-4-((2-methoxy-3-(1-methyl-1H-1,2,4-triazol-3-yl)phenyl)amino)-N-(methyl-d3)pyridazine-3-carboxamide, Compound I, which is currently in clinical trials for the treatment of autoimmune and autoinflammatory diseases such as psoriasis.

This invention belongs to the field of microbiology and relates to a lysobacterium P18 and its applications. Lysobacterium P18 belongs to the genus *Lysobacter* and was deposited with CGMCC on June 7, 2024, with accession number NO. 30877. The lysobacterium P18 provided by this invention has a growth-promoting effect on tomatoes.

This invention discloses a method for intercropping multiple forage grasses in citrus orchards. Addressing the drawbacks of traditional clean cultivation management and single-grass intercropping in citrus orchards, it employs a mixed sowing of four varieties: ryegrass, white clover, gnaphalium affine, and milkvetch. It includes standardized steps for orchard pretreatment, seed pretreatment, precision sowing, seedling management, growth period management, forage harvesting and returning to the field, and water and fertilizer regulation. Strict control of parameters at each stage is maintained, and a specific ratio of compound bio-fertilizer is selected to optimize the citrus water and fertilizer management scheme. This invention achieves year-round closed coverage of the citrus orchard through the complementary characteristics of the four forage grasses, improving the control rate of noxious weeds, reducing soil erosion, increasing soil organic matter content, and increasing citrus yield per acre. It combines the advantages of improving soil fertility, suppressing weeds and conserving moisture, improving quality and efficiency, and promoting a green and ecological environment, thus constructing a highly efficient and sustainable citrus ecological planting system.

The application discloses a bamboo shoot yield-increasing additive and a preparation method thereof, and belongs to the technical field of bamboo shoot cultivation, and comprises bitter bamboo sawdust, chitosan nano selenium, corn starch, ethanol, pectin, calcium chloride, enzyme, mannose and aloe-emodin. The prepared bamboo shoot yield-increasing additive is mixed with soil to serve as a cultivation substrate for cultivating bamboo shoots, so that the growth of the bamboo shoots can be better promoted, the growth cycle of the bamboo shoots can be shortened, the yield of the bamboo shoots can be improved, and finally, the cultivated bamboo shoots have good yield and selenium content.

The utility model provides a kind of auxiliary device for deep trench etching, it is related to chip processing field.Processing equipment main body is embedded in the top of shell, and suction equipment is embedded in the outer wall side of shell, and the front end of seal door is movably connected with the front end of seal door, and the front end of seal door is embedded with control end, the inside one side of shell is provided with limiting mechanism;The limiting mechanism includes support platform, the inside of shell is penetrated by the support platform, and the top of support platform is provided with setting groove, and the inside of setting groove is provided with partition groove.The utility model cooperates through support platform, setting groove, partition groove, suction hole, suction pipe and sleeve, can be fixed to chip by the condition of negative pressure formed by suction, avoid from the chip upper limit, influence the comprehensiveness of etching, and a plurality of partition grooves can be set to process a large number of chips simultaneously, improve the output of product, and sleeve is convenient for the disassembly and maintenance of support platform.

This application relates to the field of biotechnology and discloses a purification process for the OAS1-Cas12aREC fusion protein, specifically including its encoding gene, expression vector, and purification process. This application achieves optimal matching between the encoding gene of the OAS1-Cas12aREC fusion protein and the E. coli translation system by targeted optimization, eliminating translational bottlenecks and unfavorable elements, thereby increasing the yield and purity of the OAS1-Cas12aREC fusion protein. Furthermore, it optimizes the reagents used in the protein purification process to further promote the yield and purity of the OAS1-Cas12aREC fusion protein, while also preventing protein oxidation and stabilizing the protein structure, making it less prone to denaturation.

This invention discloses a MsMYB-ZD The application of genes in regulating alfalfa leaf size and yield falls under the field of plant genetic engineering. This invention utilizes transcriptome analysis to clone a gene that regulates alfalfa leaf size. MsMYB-ZD Transcription factors; experimental data show that, MsMYB-ZD The gene exhibits a gradually upregulated expression pattern during leaf development; compared to wild-type alfalfa Gongnong 1 (1-16) individual plants, overexpression... MsMYB-ZD MsMYB-ZD The leaf area of ​​the transgenic plants increased by an average of 77%, and the expression level of the transgenic lines showed a significant positive correlation with the leaf area. The fresh grass yield of the transgenic plants increased by 158.7%, and the dry grass yield increased by 207.5%. These results indicate that this gene is of great significance for improving alfalfa yield.