51 results about "Coronatine" patented technology

The invention discloses a drought-resistant agent and application thereof. The plant drought-resistant agent comprises the active ingredients such as coronatine (COR) and S-abscisic acid (S-ABA). Compared with the present substance for improving the drought resistance of crops, the drought-resistant agent has the following characteristics: unexpected synergy can be obtained through COR, S-ABA and other plant growth adjusting substances which are added on the basis of the COR and S-ABA; an expected addition effect on the growth of crops and the adjustment of the activities can be exceeded, and on one hand, the crop application range can be enlarged, and on the other hand, the application concentration of coronatine, S-ABA and other plant growth adjusting agents can be reduced; and moreover, under the condition that the concentration of a single compound is lower than the effective concentration, a good adjusting degree on the growth of plants can be obtained through the plant growth adjusting agent.

Stable genetically engineered bacterial strains that overproduce coronatine are provided. The stable strains can be successfully cultivated to overproduce coronatine at temperatures that are suitable for large scale, commercial preparations of coronatine. The overproducing strains are also non-pathogenic. An exemplary strain is Pseudomonas syringae APV1, which successfully overproduces coronatine at 26° C. Methods of optimizing culture conditions for coronatine production from the novel stable overproducing strains are provided, as are methods for using the overproducing strains to induce abscission and increase taxane production.

The invention discloses a method for extracting coronatine from fermentation liquor by using a membrane separation technique, comprising micro-filtration and hyper-filtration steps: filtering a fermentation liquor containing coronatine by a micro filtering membrane with the aperture of 0.1-10 Mum so as to remove impurity particles and thalli; then filtering the liquor by a hyper-filtration membrane with the aperture of 0.001-0.1 Mum so as to remove macromolecule impurities of proteins, nucleic acids and colloid granules to obtain clear filtrate; and then carrying out common resin exchange or column chromatography, concentrating and crystallizing to obtain the coronatine. In order to improve the effect of separating purification, pre-treatment can be added before the micro-filtration, namely, the coronatine fermentation liquor is filtered though the filtration membrane with the aperture of 1-10 Mum; nano-filtration can be added after the hyper-filtration, namely, the effluence liquor is filtered by a nano-membrane system with cut-off molecular weight of 400-800. The method in the invention has simple operation and high yield which can be over 90%; in addition, the method has no influence on the structure and activity of the coronatine; and has low energy consumption and low pollution, thus completely satisfying the requirements of industrialized production.

The present invention provides a new bacterial strain capable of fermenting and producing high-component coronatin, that is, Pseudomonas syringae (Psendomonas syringae) Y-2018 bacterial strain, and the preservation number is CGMCC NO.1105. The weakness of the wild strain that must be low temperature to accumulate coronatin. The present invention also provides a method for industrialized production of coronatin, which comprises preparing the Pseudomonas syringae Y-2018 strain through slant strains, culturing the seeds in shaking flasks and aerated fermentation to obtain a fermented liquid containing coronatin. Concentrate, extract, crystallize and recrystallize to obtain crystal powder and non-crystallized mother liquor. This method can use organic nitrogen medium for fermentation, and the use of organic nitrogen not only increases the amount of bacteria, but also can synthesize more coronatine, thereby overcoming the defects that wild strains are not resistant to organic nitrogen, and organic nitrogen inhibits the accumulation of coronatin .

The invention relates to a genetically engineered bacterium (Pseudomonas syringae soybean pathogenic variety CGMCC 1276), its construction method and its use. The engineering bacterium is obtained by inserting the Tn5 transposon S17-1 into the genome of the starting bacterium to inactivate its temperature control gene. The strain can be used for industrial fermentation to produce coronatine at the fermentation temperature of 26°C-28°C. The yield is similar to that of the starting bacteria (fermentation temperature is 18°C), and can reach 35mg / l-40mg / l.