706 results about "Taxus mairei" patented technology

The invention discloses an extractive of Taxus chinensis var.mairei leaves and a preparation method. The extractive is a straw yellow powder. The powder contains sciadopitysin the weight content of which is 65-70 percent; the sciadopitysin contains the following five components: 3-O-rutin-quercetol, 3-O-rutin-campherol, 3-O-rutin-myricetin, 7-O-glucose-campherol and 7-O-glucose-quercetol, and the total weight content of the five components in the sciadopitysin is 40-60 percent. The extractive is prepared by the preparation method comprising the steps of baking, crushing and screening, immersing into an alcohol dissolvant, ultrasonically extracting while stirring, decompressing and concentrating an extracting solution to remove alcohol, extracting with chloroform and degreasing, water phase concentrating and drying by taking fresh Taxus chinensis var.mairei leaves as raw materials. The extractive of Taxus chinensis var.mairei leaves has the functions of improving heat microcirculation, resisting arrhythmia, lowering myocardial oxygen consumption and the like and having the therapeutic and health-care function on diseases of cardiovascular system; and the preparation method has low energy consumption, short extracting time, few impurities and higher extraction efficiency.

The invention discloses an original ecology organic cultivation integrated technology under dendrobium officinale. The technology full simulates the wild environment of natural growth under the dendrobium officinale mountainous area, no greenhouse facility is needed, vegetation which is manually rimmed is used for overshadowing, dendrobium officinale tissue culture seedling are directly transplanted to culture medium which is provided with excellent breathability and is manually prepared under wood after being disinfected, or is directly transplanted to a loose ground which is in a natural state and is rich of organic humus under wood for open type, wild-mimic healthy and ecological planting, biogas slurry, silkworm feces and spring water which are dedicated for the dendrobium officinale are applied, the water and fertilizer supply of the dendrobium officinale is ensured, American taxus chinensis is moderately interplanted in a base for driving pests, the survival rate of transplanting of the dendrobium officinale tissue culture seedling can reach over 95 %, the effective elements are high in content, the quality is excellent, no chemical drug residue is produced, the management is convenient and the investment is smaller. The specific steps comprises base selection, base cleaning, biogas slurry preparation, mist spray irrigation system installation, woodiness medium manufacturing, culture medium pavement, tissue culture seedling disinfection, transplantation, base sterilization, water and fertilizer management, pest control and harvesting.

The invention provides a method for breeding and cultivating Taxus chinensis var mairei, and aims at improving the survival rate of Taxus chinensis var mairei bred through asexual cuttage. According to the method, Taxus chinensis seedlings are asexually bred through cuttage, and then large seedlings of Taxus chinensis and sapling grove are obtained through transplanting and planting to enlarge the cultivated area of Taxus chinensis var mairei. By adopting the specific cultivation management measures of Taxus chinensis var mairei, the technology system for breeding and transplanting Taxus chinensis var mairei is established, and an approach for the artificial reproduction of Taxus chinensis var mairei is hewed out, so that large-scale industrial production of Taxus chinensis var mairei becomes possible, thereby satisfying the market demand for Taxus chinensis var mairei, and providing a good test system for the protection of endangered wild plant resources.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

Methods for obtaining paclitaxel from plants containing paclitaxel are disclosed. Plant material is first obtained from plants containing paclitaxel. Paclitaxel is then extracted from the plant material. Subsequently, paclitaxel is separated from the paclitaxel extract using a series of column chromatography separation steps to obtain at least one fraction containing paclitaxel. The paclitaxel in a fraction that is obtained from the final chromatography step is crystallized. Using these methods, high purity paclitaxel may be efficiently obtained from plants containing paclitaxel.

The invention relates to a cuttage breeding method of taxus chinensis in northeast, comprising the following steps: selecting a one-year-old branch as a branch for cuttage; concealing the branch at ashade place, and carrying the branch to a destination after the branch is packaged by a plastic bag; retaining 1-2 leaves on the upper end of each nursery stock stalk when shearing a seedling, shearing the parts longer than 3cm of the retained leaves, and also shearing leaves inserted into the stalk positioned in the middle of a seedling bed; disinfecting a land before the cuttage so as to reducerhizoctonia solani kuhn, and carrying out the cuttage from the middle ten days of October to the first ten days of November or from February to March; using a light shielding net of 80 percent to shield light without directly shining with the sun during the daily management of the seedling; controlling the soil water content at 35-45 percent; and paying attention to the water drainage of the seedling in rainy days and intertillage weed control in ordinary days, and preventing and curing diseases of the seedling in time by chemical agents. By adopting the cuttage breeding method, the taxus chinensis in northeast has high survival rate, fast breeding cultivation and high disease resistance.

The invention discloses a method for cuttage of taxus chinensis, which comprises the following steps of: cutting out a cuttage branch, and soaking the cuttage branch in chemical slurry to disinfect; carrying out cuttage on the cuttage branch which is soaked and disinfected onto a seedling bed to implement non-fertilizer breeding until epidermis on a cutting wound of the cuttage branch grows and heals; spraying a nutrient solution onto the seedling bed to implement fertilizer breeding, so as to promote growth of roots and leaves from the branch; and after 3-6 months, implementing normal nursery management or transplantation. According to the method for cuttage of the taxus chinensis, the chemical slurry which is used for soaking the cuttage branch is prepared by mixing yellow soil, a plant peel promoting agent, a rooting agent, a sterilizing agent and water; the chemical slurry coated on the cutting wound part of the cuttage branch can effectively prevent the water and the nutrition in the branch from evaporating or losing, and can disinfect the cutting wound part of the branch and promote healing and corrosion prevention of fresh skin of the wound, and therefore, the survival rate of the cuttage branch is further improved.

Disclosed is a culture composition containing micro and macro-ingredients, vitamins and other supplements such as phytagel, sucrose, picloram. The medium is useful in the formation and development of callus lines of Taxus sp., and resulting in production of taxol in high amounts.

The invention relates to a method for extracting paclitaxel from taxus chinensis branches and leaves, and the method comprises the following steps of: (1) soaking 1000 kg of pulverized taxus chinensis branches and leaves by using 5 m<3> of methanol, and filtering; (2) decoloring filter liquor; (3) decompressing and concentrating a decolored solution, and extracting; (4) degreasing and dewaxing extract liquor, crystallizing, depositing, filtering, and drying to obtain a primary product; (5) removing impurities contained in the primary product in a silicagel column, carrying out gradient elution, collecting paclitaxel-containing elution flows, and decompressing and concentrating to obtain a crude product; (6) stirring the crude product into silica gel, packing, carrying out gradient elution, collecting the paclitaxel-containing elution flows, and decompressing and concentrating to obtain a semi-finished product; (7) dissolving the semi-finished product, adding petroleum ether, standing, filtering, and drying to obtain a semi-finished product with the content more than or equal to 30%; (8) removing the impurities contained in the semi-finished product obtained from the step (7) in a silicagel column, carrying out gradient elution, collecting the paclitaxel-containing elution flows, and decompressing and concentrating to obtain a semi-finished product with the content more than or equal to 95%; and (9) dissolving the semi-finished product obtained from the step (8), filtering, adding normal hexane, standing, carrying out suction filtration; and drying to obtain a finished product with the content more than or equal to 99%.

The invention belongs to the technical field of plant budding, particularly relates to a method for stopping Taxus chinensis var. mairei seed dormancy, and solves the technical problem of slow budding, low budding rate and the like. The method includes the steps of A, soaking Taxus chinensis var. mairei seeds in sulfuric acid 55-65% in mass concentration for 2-4 hours, and washing the soaked seeds with flow water for 12-36 hours; B, soaking the acid etched seeds in pre-prepared solution for at least 3 hours, and soaking the soaked seeds in clean water for at least 3 hours; and C, performing low-temperature stratification or variable-temperature stratification to the seeds soaked by clean water. The method has the advantages that the method is time-saving, effective and economical, the problems that dormancy is deep, budding is difficult and budding rate is low are solved, standards are provided for seed seedling market circulation, seed quality is guaranteed, and great significance is brought to breeding cultivation development of Taxus chinensis var. mairei.

Belonging to the technical field of medicine extraction, the invention discloses a method for extraction of paclitaxel from taxus chinensis. The extraction method provided in the invention consists of: using a methanol solution to conduct warm soaking extraction of taxus chinensis bark or branch and leaf powder, then carrying out chloroform extraction, performing silica gel column chromatography twice, implementing elution with a chloroform-methanol gradient solution and an n-hexane-acetic acid gradient elution respectively, and removing polar impurities so as to obtain a paclitaxel crude product; and further, carrying out third normal phase column chromatography, taking chemically treated silica gel as a stationary phase to effectively separate paclitaxel, recrystallizing the paclitaxel obtained from the column chromatography, thus obtaining a paclitaxel pure product with purity of over 98% pure product, with a process transfer rate being more than 80%. All the reagents used in the extraction method belong to the industrial grade, and the method provided in the invention has the advantages of wide applicability, strong innovation, simple process, low separation cost, high product yield, thus having innovative production value for industrial separation and production of paclitaxel.

The invention relates to a cutting and breeding method for taxus chinensis, and belongs to the technical field of plant breeding. The cutting and breeding method comprises the following steps: scion wood selection, scion wood treatment, scion wood cutting, management after cutting, seedling transplantation, and management after transplantation. The adopted cutting and breeding method has the advantages that the cutting and breeding method with high survival rate is provided for taxus chinensis breeding in the south, the planting range of taxus chinensis is enlarged, and the difficult problem of taxus chinensis resource shortage can be favorably solved.

The invention provides a taxus chinensis propagating method. The method is characterized by comprising the following steps that 1, taxus chinensis branches are selected; 2, bactericide is used for treating the branches; 3, a plant growth regulator is used for treating the branches; 4, water culture is adopted for inducing the branches to take root; 5, a nutrient solution is added for curing. By the adoption of the method for propagating taxus chinensis, restriction of cultivated land resources can be gotten rid of, and the propagation area is increased; meanwhile, the influence on the survival rate from external environment is reduced to the maximum, time for callus forming and rooting is shortened, the stem cutting survival rate is remarkably increased, the restriction that due to poor environmental factors such as temperature, humidity, soil and water quality, the stem cutting survival rate is influenced by an original propagation means is broken through, the current situation that taxus chinensis genetic resources are scanty can be relieved, and great economic value can be created.

The invention relates to a grafting cultivation method for taxus chinensis. The grafting cultivation method is characterized by comprising steps of a, selecting taxus chinensis saplings with required characters, selecting healthy and plumb scion buds on scion branches where leaves are removed and only leaf stalks are remained, transversely cutting the upper side of each scion bud once, cutting the lower sides of the scion buds from bottom to top, connecting each cut opening with the bottom of a corresponding transverse cut opening to form a big-end-up bud slice and taking off the bud slices; b, selecting common taxus chinensis seedlings as rootstocks, transversely cutting each rootstock once, and longitudinally cutting a transverse cut opening of each rootstock downwardly once to form a 'T'-shaped cut opening on the rootstock; c, performing cutting grafting on the buds, in other words, holding each bud sheet by the left hand of an operator, holding a scion bud knife by the right hand of the operator, blocking bast of the rootstocks by the aid of a handle of the scion bud knife, inserting the bud sheets into the open 'T'-shaped cut openings, pressing the leaf stalks of the bud sheets by hands of the operator and tying the leaf stalks of the bud sheets by plastic films; (4), removing branches of the rootstocks straightaway after cutting grafting is performed on the buds, and timely removing sprouts and coppice shoots of the rootstocks. The grafting cultivation method has the advantages of good bud grafting effect, high survival rate and capability of quickly cultivating large quantities of seedlings.

The invention discloses a grafting and cultivation method of Taxus chinensis, and belongs to the technical field of traditional Chinese medicine material planting. The method includes preparation before grafting, grafting operation and management after grafting. The preparation before grafting, comprises the steps that grafting time is June to July, a scion is collected 15-30 d before grafting, the whole scion is sealed with paraffin and is put into wet sand in a cool place for preservation. The rafting operation comprises the steps of a, treatment of cutting scion, wherein the grafting opening is dipped in a root growing foliar fertilizer for standby application; b, grafting, wherein stopping cutting at the grafting part, one side of a rootstock section is cut into a concave triangular rootstock groove using a grafting knife, the scion is taken out, the other end of the rootstock section is cut into a convex triangle corresponding the concave triangle rootstock groove, the scion is embedded into the rootstock groove, both sides of the rootstock groove are made to be adjoined, and a callus anti-corrosion film is smeared. The management after grafting comprises the steps of a, unbinding and removing burgeons; b, applying the fertilizer and moisture management; c, grafted seedling pest control. According to the grafting and cultivation method of Taxus chinensis, grafting is conducted in June to July, the method is suitable for grafting of a big rootstock, and has the advantages of being fast in shooting, early in formation of crowns, smooth in grafting healing part and high in grafting survival rate.

The invention belongs to the technical field of garden planting, and particularly relates to a high-survival rate planting method of taxus chinensis. The high-survival rate planting method comprises the following five steps: performing field conditioning, digging holes and preparing a field, selecting seedlings, planting, and performing water and fertilizer management, wherein in the field conditioning process, an organic fertilizer is early applied so as to ensure the organic matter content and the microorganism content in soil in the planting process; in the seedling process, roots are uniformly adsorbed with sterol substances in rapeseeds soaked by rapeseed soaking liquid, growth and development of the roots in the planting process can be effectively promoted, germination of side roots is promoted, and the survival rate of the taxus chinensis in the planting process is improved; in the planting process, the moisture content of the soil is strictly controlled, the fact that the soil is not too dry nor too wet is ensured, and the taxus chinensis has a relatively strict requirement on moisture in the growing process and is not resistant to waterlogging, so that in the planting early stage, strict control over the moisture content of the soil can satisfy the growth need of the taxus chinensis to the maximum extent, the survival rate is ensured, and a root rotting phenomenon is avoided. The taxus chinensis planted by the high-survival rate planting method has the survival rate higher than 98%.

The invention discloses a method for separating paclitaxel and related taxane substances, and belongs to a technical method for chemically separating natural products and forest products. The method is characterized by comprising the following steps of: (1) uniformly mixing two kinds of macroporous resin in a certain mass ratio, and loading in a glass column to obtain a mixed resin packed column; and (2) dissolving a crude extract of taxus chinensis in a solvent in a ratio of 1:5 with stirring, adding an adsorbent, stirring until the solvent is completely volatilized, loading in the top end of the packed column, adding a gradient eluent for elution, collecting fractions in various gradients, and concentrating to obtain the paclitaxel and related taxane substances, which have obviously improved content. The raw materials used in the method are wide in range, and various crude extracts containing the paclitaxel and related taxane substances can be separated; the resin is commercially common resin, cheap and readily available; and the solvent is a common solvent which is low in toxicity and easy to recover. The production cost is low.

The invention relates to a method for using specific resin and silicon gel column chromatography purification via negative pressure cavitation water extraction to high-efficiency produce 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel, which uses taxus branch leaves as raw material, dries and breaks to process negative pressure cavitation water extraction, filters and uses AB-8 resin to process dynamic absorption on filter liquor to concentrate 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel and process further purification via middle pressure silicon gel column chromatography, to obtain the 10-deacetylbaccatin III of at least 60% purity and 7-xylose-10-deacetylpaclitaxel of at least 67% purity. The invention can use renewable taxus branch leaves to protect ecological resource and effectively extract effective component and biological semi synthesis precursor materials, with simple operation, support for industrial application and important industrial production value.

The invention refers to a plant extract to prevent and cure diabetes, extracted from Taxus spp, especially Taxusyunnanensis Cheng et L.K.Fu, Taxus chinensis var.mairei(Lemee et Levl)Cheng et L.K.Fu, etc. It refers to making method and usage. The content of main component of the plant extract powder, myo-inosite derivant compound, can reach above 60%.

The present invention relates to a yew branch and leaf extract, an extraction method and applications thereof. The yew branch and leaf extract is extracted by adopting branches and leaves of Taxus chinensis var. mairei as raw materials, and comprises cephalomannine and 7-epitaxol, wherein the active ingredients provide an inhabitation effect for squamous cells in skin cancers, and especially provide significant treatment effects for anal squamous cell carcinoma, esophagus squamous carcinoma and tonsil squamous cell carcinoma. According to the present invention, resource advantages of the yew are utilized to deep develop, utilize and research the Taxus chinensis var. mairei; and a yew anti-skin cancer component extraction method with a characteristic of simple operation is provided, and an effect of the component in anti-skin cancer application is determined.