The invention discloses a method for domesticating and transplanting a taxus chinensis var mairei tissue culture seedling. The method comprises the following steps of (1) selecting the tissue culture seedling by means of selecting the taxus chinensis var mairei tissue culture seedling with a well-grown root system and more than one true leaf; (2) preliminarily domesticating the tissue culture seedling by means of putting a tissue culture container with the tissue culture seedling into a greenhouse for 3 to 5 days; (3) preparing a transplanting container and a culture substrate by means of selecting a seedling culturing box, and filling vermiculite, turf or nonwoven-fabric wrapped fresh coconut chaff in holes of the seeding culturing box; (4) transplanting and hardening seedling by means of planting the tissue culture seedling in the middle of the culture substrate obtained in the step (3), watering sufficiently, closing a seedling culturing box cover, putting the box into the greenhouse with 50 percent of sunshield screen, culturing at an environmental temperature of not more than 35 DEG C, and opening the seedling culturing box cover and performing a conventional seedling management mode after two weeks, wherein the survival rate of transplanted tissue culture seedlings reaches 100 percent at most. The method disclosed by the invention has the advantages that the disadvantages in the prior art are overcome, and the effects of simplicity in operation, low cost, high efficiency, stability and reusability of the seedling culturing box are achieved.