30 results about "Taxus chinensis var. mairei" patented technology

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

The invention discloses a taxus chinensis var mairei artificial propagation technology, and belongs to the scientific and technological field of rare plant protection. The taxus chinensis var mairei artificial propagation technology aims to solve the problems that taxus chinensis var mairei is complicated in growth characteristic, low in growth speed under natural conditions and poor in regeneration capacity. Through countless tests for many years, taxus chinensis var mairei artificial propagation key indicators are captured, the survival rate, the propagation speed and the growth speed of taxus chinensis var mairei seedlings are improved, and the taxus chinensis var mairei artificial propagation technology is made to reach the domestic advanced level, wherein a hard-branch mass production type cutting technology fills up a provincial blank.

The invention discloses a group of combined strains for highly producing baccatin III and a method for producing baccatin III, belonging to the technical field of biology. The combined strains for highly producing baccatin III are combined strains of baccatin III production strains and paclitaxel production strains, wherein the baccatin III production strains are Taxus chinensis var mairei endophytic fungi no.79, and paclitaxel production strains are Taxus media endophytic fungi Z58 or Taxus india endophytic Gliocladium SP. By sequentially carrying out independent culture and mixed culture on the baccatin III production strains and the paclitaxel production strains, the yield of baccatin III in an fermented object is substantially increased; by virtue of metabolic pathway complementation and a feedback inhibition theory, the yield of baccatin III is greatly increased and is far higher than that of an independent culture manner. According to the method, the industrial fermentation culture and production of baccatin III by virtue of endophytic fungi are realized, relatively cheap raw materials are provided for the production of paclitaxel by virtue of a semi-synthesis method, and the production cost is lowered; the dependence on the Taxus chinensis resource is reduced, and the damage to the environment is alleviated.

The invention relates to a method for promoting growth of camptotheca acuminate. The method comprises the operating steps of obtaining a methyl alcohol extracting solution concentrate of branches and leaves of taxus chinensis var mairei, and irrigating the camptotheca acuminate with a water suspension of the methyl alcohol extracting solution concentrate. The method for promoting growth of the camptotheca acuminate is simple and high in operability, and by the adoption of the method, the whole plant biomass of the camptotheca acuminate can be improved by more than 18%, and the content of camptothecin in the camptotheca acuminate is increased by more than 12%.

The invention discloses a bionic double-protection seedling technology for taxus chinensis var mairei. The bionic double-protection seedling technology is mainly characterized in that sterilized seeds are sowed in a sterilized garden, and are covered; a shade shed is built immediately in the garden; and bryophyte plants are sowed on a seedbed in time to cover the seed to construct a bionic natural ecological environment to ensure that the seedlings can normally develop and grow so as to achieve the purpose of fast growing, high quality and high yield. The invention has the advantages of low cost for seedling culture, fast growing speed of seedlings, and high rate of emergence, and increases the yield by 30-40 percent compared with traditional open ground seedling culture; and over 80 percent of the seedlings can be qualified.

A taxus chinensis var mairei afforestation method sequentially comprises the following steps that (a) afforestation land is selected and planting points are configured; (b) land preparation and planting are performed; (c) China fir coppice shoots are retained, and independent root systems are promoted; (d) young timber tending and fertilization are performed; (e) the canopy density of a China fir forest canopy layer is adjusted and controlled; (f) taxus chinensis var mairei forest establishment and tending are performed; (g) China fir coppice is achieved with a standard system. The taxus chinensis var mairei afforestation method includes the single steps including afforestation land and planting point configuration, land preparation and planting, China fir coppice shoots retaining and independent root system promotion, young timber tending and fertilization, adjustment and control of the canopy density of a China fir forest canopy layer, taxus chinensis var mairei forest establishment and tending and China fir coppice the standard system, the nutrition and space conditions of the afforestation land are fully utilized, the afforestation land output is remarkably improved, early-stage earnings are obtained, and the problems of low preserving rate, difficult forest and lumber establishment, long earning time and the like of taxus chinensis var mairei afforestation are solved.

The invention relates to a taxus chinensis var mairei tree and seedling interplanting method. The method is characterized by comprising the following steps that firstly, fertilizing and plowing are conducted after a field piece is cleaned; secondly, a gully is dug every other distance a in the longitudinal direction of the field piece, and a transverse furrow is dug every other distance b in the transverse direction of the field piece, wherein a ranges from 15 m to 20 m, and b ranges from 20 m-25 m; thirdly, ridges are made, the width d of the ridges ranges from 1.2 m to 1.3 m, the length e of the ridges ranges from 5.5 m to 6 m, the distance f between the ridges ranges from 30 cm to 40 cm, and small furrows are dug; fourthly, field planting is conducted, threes with the ground diameter ranging from 8 cm to 9 cm and the height ranging from 4 m to 5 m are selected, two trees are planted on each ridge, the planting distance g ranges from 2.5 m to 3 m, two to three year old seedlings of small trees are selected, the planting distance h of the small trees ranges from 45 cm to 50 cm, the row distance i ranges from 50 cm to 60 cm, 20-30 small trees are planted on each ridge, before field planting, the root systems of the large trees and the small trees are soaked in an ABT1 rooting powder solution with mud, after field planting, root fixing water is used for thorough watering, and the soil moisture obtained after watering of the roof fixing water ranges from 40% to 60%. According to the taxus chinensis var mairei tree and seedling interplanting method, the utilization rate of the land can be greatly increased, the cost of setting up a sunshade net can also be saved for planting taxus chinensis seedlings, and the seedling growing cost is reduced.

The invention discloses a method for improving cutting survival rate of Taxus chinensis var mairei. The method includes soil preparation, seedbed making, rooting medium preparation, cutting preparation and seeding raising by cutting. The preparation process of rooting medium includes picking tender willow leaves, smashing them, performing filtering to acquire tender leaf juice, adding 0.18 to 0.2kg of the tender leaf juice to 1L of mountain spring water and adding 0.23 to 0.28kg of edible white granulated sugar to 5L of mountain water; mixing the two solutions and allowing standing for 4 to 6 hours. By the method the survival rate of Taxus chinensis var mairei adopted as cutting strips can be effectively improved, the survival rate can reach higher than 95 percent, working efficiency of farmers are improved and manufacturing cost is correspondingly decreased.

The invention relates to a building method of a taxus chinensis var mairei cutting orchard for producing erect panicle. The method comprises the steps of removing top tips, adjusting crown bodies, bending main trunks, conducting exposure to promote germination, picking scions and conducting management after picking the scions. By means of the building method, the cutting orchard is short in development cycle, easy to operate and low in cost and well solves the technical problem of common taxus chinensis var mairei cutting seedlings that crowns easily deflect due to a position effect; meanwhile, the yield of the scions of the cutting orchard is greatly increased, the quality of the scions is greatly improved, the method has the advantages of being easy to operate, easy to popularize and thelike, meets large-area breeding and cultivation requirements and can increase the good variety breeding level of taxus chinensis var mairei.

The invention discloses a traditional Chinese medicine for treating lung cancer and a preparation method thereof. The traditional Chinese medicine is prepared from the following components: radix tetrastigme powder, a ganoderma lucidum extract, broken ganoderma lucidum spore powder, broken pine pollen, taxus chinensis var mairei, edible tulip, radix actinidiae chinensis, corydalis amabilis, phyllanthus emblica and rhizoma phragmitis.The effects of supporting the healthy energy and inhibiting tumor cells are achieved.The traditional Chinese medicine has a good effect of assisting in improving immunity after radiotherapy and chemotherapy, and has the effects of supporting the healthy energy and inhibiting tumor cells.

The invention discloses a taxus chinensis var mairei container seedling substrate. Thoroughly decomposed vinegar residue is added into fresh vinegar residue and sawdust; urea is also added in the sawdust; primary fermentation is performed; after the mixing, perlite is added; secondary fermentation is performed to form the substrate. The volume weight of the prepared substrate is 0.40+ / -0.02g / cm<3>; the total porosity is 62.8+ / -2.2 percent; the water holding porosity is 38.8+ / -1.1 percent; the air porosity is 24.0+ / -1.3 percent. The invention also discloses a preparation method of the taxus chinensis var mairei container seedling substrate. The substrate is mainly prepared from organic waste materials of the vinegar residue and the sawdust; the preparation process is simple; the operation is easy; the related raw materials are mainly organic waste fermentation products; the consumption of microbial inoculums is greatly reduced; the production cost is reduced; meanwhile, the integral utilization of waste resources is realized; the environment pollution is reduced. The prepared substrate has a good seedling effect; a conventional grass carbon substrate can be replaced; the cost is low; the market prospects are wide; the substrate prepared according to the preparation method has the advantages that the seedling height, the ground diameter and the total branch number of taxus chinensis var mairei container seedlings can be obviously improved; the chlorophyll content of leaves is improved.

The invention discloses a forest genetic breeding method for taxus chinensis var mairei. The method comprises the following steps of pollen collecting, pollination, fertilizer applying, fruit bearing, sand storage, seeding and transplanting. The method solves the problem that pollination and breeding are difficult due to desynchronization of flower phases among taxus chinensis plant seeds, and is easy and convenient to implement, and a large amount of production practice is carried out in a spraying mode. The method has important and profound significance on deep development and wide application of taxus chinensis var mairei plant resources, and the application prospects are wide.

The invention discloses a cultivation method of taxus chinensis var mairei. The method comprises the steps that a soil matrix is prepared; a flowerpot is selected, wherein a drain hole is formed in the bottom of the flower pot; shoots of the taxus chinensis var mairei are selected; transplantation is conducted; maintenance in a greenhouse is conducted; household maintenance is conducted. The cultivation method of the taxus chinensis var mairei has the advantages that the method is simple, raw materials are easily obtained and the price is low; the soil matrix is rich in organic matter and has breathability and water retention effects, indoor growth of the taxus chinensis var mairei used as ornamental potting can be promoted, and the method greatly improves the indoor maintenance survival rate and survival period of the taxus chinensis var mairei.

The invention discloses a method for shoot cuttage propagation of wild taxus chinensis. The method comprises the steps of treating cuttage shoots, treating seedling beds, carrying out shoot cuttage and carrying out later-stage management. The method has the beneficial effects that the survival rate of cuttage propagation of the taxus chinensis is greatly increased, and the survival rate of cuttage shoots of the taxus chinensis is 95% or more, so that the extremely precious taxus chinensis, which is protective to nations and even the world, is protected; through reasonably designing, weeding, shading and seedling stage management, the cost of seedling raising of taxus chinensis var mairei is reduced, and the overall survival rate is increased; and cuts of the cuttage shoots of the taxus chinensis are soaked in a rooting solution, so that the survival rate of the cuttage shoots is increased.

The invention discloses a preparation and application method of a taxus chinensis var mairei fast-growing synergistic organic fertilizer, the fast-growing synergistic organic fertilizer is applied in the taxus chinensis var mairei vegetative growth period, the growth of taxus chinensis var mairei is promoted, and the medicinal component content of taxus chinensis var mairei leaves is increased. The organic fertilizer prepared by combining resource recycling of the biogas residues, the straw and the rice straw is balanced in nutrition, can improve soil properties, provides comprehensive nutrient elements for growth of taxus chinensis var mairei, promotes growth of taxus chinensis var mairei, and can effectively increase the medicinal component content of taxus chinensis var mairei. The method effectively solves the problems of high energy consumption, recycling of plant waste residues and environmental protection in the medicinal material planting and treatment process, and has good popularization and application prospects.

The invention provides a wild imitation method for artificially improving the germination rate of natural taxus chinensis var mairei population seeds and promoting seedling renewal, and relates to the technical field of seedling raising. According to the wild imitation method, in the process of artificially promoting natural taxus chinensis var mairei population recovery, taxus chinensis var mairei seeds stored in sand for one year are sown on the ground without herbaceous layers and with dry branches and fallen leaves removed, and earthing is conducted for 2-6cm. It is proved in the embodiment that the germination rate of the seeds can be remarkably increased (the germination rate of a control group is 0%) by sowing and earthing on the ground without the herbaceous layers and with the dry branches and the fallen leaves removed, and the germination rates of the seeds with the soil covering thicknesses of 2cm, 4cm and 6cm can reach 1.57%, 3.57% and 2.28% respectively. The wild imitating method disclosed is extremely important for promoting near-natural germination and updating of seeds of the natural taxus chinensis var mairei population and recovering and expanding of endangered natural populations.

The invention discloses a forest genetic breeding method for taxus chinensis var mairei. The method comprises the following steps of pollen collecting, pollination, fertilizer applying, fruit bearing, sand storage, seeding and transplanting. The method solves the problem that pollination and breeding are difficult due to desynchronization of flower phases among taxus chinensis plant seeds, and is easy and convenient to implement, and a large amount of production practice is carried out in a spraying mode. The method has important and profound significance on deep development and wide application of taxus chinensis var mairei plant resources, and the application prospects are wide.

The invention relates to an aging method capable of debitterizing and dearomatizing an orange brandy. The aging method comprises the following steps: selecting Taxus chinensis var mairei branches, phyllostachys pubescens and kudzu roots as combined aging accelerating materials, and pretreating; on the basis of an original wine, adding the aging accelerating materials in the following weight percents: 8-12% of Taxus chinensis var mairei branch, 8-12% of green sheath-removed phyllostachys pubescens strip and 3-4% of kudzu root strip; storing in a sealed manner under natural conditions, stirring once every other month, and performing aging treatment for 12 months or above; then filtering the qualified wine having no new wine flavor defect, and collecting; and finally, filtering, and blending to obtain the orange brandy finished product. According to the method provided by the invention, amino acid, taxol and other nutrition and health care components in the wine are increased; the debitterizing and dearomatizing procedure is integrated with the aging procedure, thus simplifying the operation and lowering the cost; and the method solves the new wine flavor defects of pungent orange aroma, spicy taste and obvious bitterness, achieves the favorable effects of removing bitterness, reducing orange aroma and accelerating aging, and improves the quality of the orange brandy.

The invention relates to a stereoscopic ecological cultivation method of taxus chinensis var mairei and prunus campanulata. According to bio-ecological property differences of fast-growing, positive and deciduous species of the prunus campanulata and non-fast-growing, negative and evergreen species of the taxus chinensis var mairei, based on the cultivation target and the cultivation period of green flower seedlings of the prunus campanulata and commercial forests of the taxus chinensis var mairei, the efficient ecological stereoscopic cultivation method is established by scientific and reasonable preparation, seedling selection, field planting, pruning, bifurcation trimming and utilization.

The invention relates to a preparation method of a mixed feed for livestock and poultry. The every 100 g of mixed feed is prepared from the following ingredients by weight: 2 g of tea, 3 g of pine needle, 10 g of oil tea cake meal, 1 g of lactuca sativa leaf powder, taxus chinensis var mairei, 50 g of corn, 20 g of wheat bran, 19 g of soybean meal, 1 g of calcium, 1 g of bone meal and 1 g of salt, green feed is matched with tea, taxus chinensis bark and pine needle, energy feed is matched with corn, wheat bran and soybean meal, protein feed is matched with oil tea cake, and root block feed is matched with calamus sweet potatoes and the like. The physique immunity function of livestock and poultry can be enhanced through the tea, white blood cells can be increased, so that the effects of resisting cancer, preventing cancer and preserving health are achieved, the pine needle leaf powder can adjust the organism functions of the livestock and poultry, the disease resistance rate can be increased, the laying rate can be increased, the oil tea cake can make the raised livestock and poultry high in disease resistance and good in meat quality, the lactuca sativa leaf powder has the effect of preventing digestive tract diseases, and the bark, branches, leaves and seeds of taxus chinensis var mairei contain taxol, so that the taxus chinensis var mairei can treat various cancers, and belongs to a good anti-cancer and cancer-preventing medicine.

The invention provides a taxus chinensis var mairei coniferous endophytic fungus strain and application thereof, wherein the strain is Pseudodidymocyrtis lobariellae KL27 and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 14, 2021, and the preservation number is CGMCC NO.22459. According to the strain Pseudodidymocyrtis lobariellae KL27, cultured fermentation broth is sprayed on taxus chinensis var mairei nursery stocks, so that biosynthesis and accumulation of paclitaxel in coniferous leaves of taxus chinensis var mairei can be effectively promoted, and the strain Pseudodidymocyrtis lobariellae KL27 has a relatively high application value.

The invention relates to a building method of a taxus chinensis var mairei cutting orchard for producing erect panicle. The method comprises the steps of removing top tips, adjusting crown bodies, bending main trunks, conducting exposure to promote germination, picking scions and conducting management after picking the scions. By means of the building method, the cutting orchard is short in development cycle, easy to operate and low in cost and well solves the technical problem of common taxus chinensis var mairei cutting seedlings that crowns easily deflect due to a position effect; meanwhile, the yield of the scions of the cutting orchard is greatly increased, the quality of the scions is greatly improved, the method has the advantages of being easy to operate, easy to popularize and thelike, meets large-area breeding and cultivation requirements and can increase the good variety breeding level of taxus chinensis var mairei.

The invention provides a cultivation method for efficient production of taxus chinensis var mairei, which comprises the following steps: firstly, transplanting and planting container seedlings, uniformly mixing peat soil, vermiculite, red soil and other matrixes according to a ratio of 5: 3: 2, planting three-year-old cutting seedlings, and then carrying out light and temperature control and nutrition control from May to October and from October to December. The method can quickly and effectively improve the annual growth amount and fruit yield of taxus chinensis var mairei plants, so that the income is increased. Meanwhile, the method has the characteristics of simplicity in operation, easiness in mastering, high income and the like.

The invention discloses a group of combined strains for highly producing baccatin III and a method for producing baccatin III, belonging to the technical field of biology. The combined strains for highly producing baccatin III are combined strains of baccatin III production strains and paclitaxel production strains, wherein the baccatin III production strains are Taxus chinensis var mairei endophytic fungi no.79, and paclitaxel production strains are Taxus media endophytic fungi Z58 or Taxus india endophytic Gliocladium SP. By sequentially carrying out independent culture and mixed culture on the baccatin III production strains and the paclitaxel production strains, the yield of baccatin III in an fermented object is substantially increased; by virtue of metabolic pathway complementation and a feedback inhibition theory, the yield of baccatin III is greatly increased and is far higher than that of an independent culture manner. According to the method, the industrial fermentation culture and production of baccatin III by virtue of endophytic fungi are realized, relatively cheap raw materials are provided for the production of paclitaxel by virtue of a semi-synthesis method, and the production cost is lowered; the dependence on the Taxus chinensis resource is reduced, and the damage to the environment is alleviated.

The invention discloses a production method of a culture medium for landscaping. The culture medium comprises the following raw materials in parts by weight: 1-3 L of distilled water, 0.5-1.5 mmol of inorganic elements, 8-24 g of glucose, 200-600 mg of vitamins, 8-24 g of agar and 10-30 mg of streptomycin. The process is mature, microelements are provided for plant growth through MgSO<4>.7H2O, KCl and NH4-N in the inorganic elements, and through Vpp nicotinic acid in the vitamins, plant metabolism and embryo development are promoted, 100 mg / L of inositol helps mutual transformation of carbohydrates, can promote growth of calluses and formation of embryoids and buds, has a promoting effect on propagation and differentiation of tissues and cells, also has an effect on formation of cell walls and accelerates root development, and the streptomycin prevents bacterial contamination and reduces material loss in culture. The whole method can be widely applied to cuttage grafting culture of taxus chinensis var mairei.

The invention relates to an artificial hybridization method of taxus chinensis. The artificial hybridization method is characterized by comprising the following steps: (1) collection of pollen: when taxus chinensis female plant is at a full-bloom stage, collecting taxus chinensis var mairei male flowers flowering on the day; (2) treatment method: thinly spreading the male flowers on white paper, covering with white paper, airing under sunshine, overturning once for every 2 to 2.5h till seeing pollen after only 1 day, and slightly sieving to remove impurities; blending pollen and clear water into pollen liquid according to the weight ratio of pollen to water being 1:(1000-2000), and then adding the following components in percentage by weight into the pollen liquid: 0.1%-0.3% of chitosan, 3%-5% of borax and 5%-10% of sugar; and (3) conducting spray hybridization through a sprayer, namely spraying once for every 3 to 4 days through the sprayer, and totally performing hybridization for three times. The filial generation obtained by adopting the artificial hybridization method of taxus chinensis not only has the rapid growth characteristics of taxus chinensis var mairei, but also has the cold-resistant and drought-resistant advantages of taxus chinensis.

The invention provides a method for increasing the survival rate of seedlings of true bud seedlings in a taxus chinensis var mairei natural population, and relates to the technical field of seedling raising. According to the method, after seeds in the taxus chinensis var mairei natural population germinate into seedlings, weeding is conducted 1-2 times from May to August. According to the method, no human intervention is carried out except weeding, proper removal of vegetation on the herbaceous layer is beneficial to survival of bud seedlings, the survival rate of the bud seedlings can be remarkably increased by removing the herbaceous layer (the average germination rate of a control group is 4.55%), and the survival rate of an experimental group is 78.07%, that is, the survival rate is increased by 73.52% compared with the control group ,and is 17.16 times of the survival rate of the control group; the survival rate of the taxus chinensis var mairei seedlings is greatly increased, near-natural updating of natural populations is facilitated, and research of a near-natural artificial promotion natural taxus chinensis var mairei population recovery mode is facilitated.

The invention discloses a method for extracting 10-DAB from taxus chinensis var mairei, which comprises the following steps: taking taxus chinensis var mairei, drying, pulverizing, screening, adding methanol according to a solid-liquid ratio of 1: (5-25) g / ml, carrying out ultrasonic extraction, centrifuging the extracting solution, taking a supernatant, and concentrating to obtain the 10-DAB-containing extract. Compared with the existing extraction technology, the method for extracting the 10-DAB from the taxus chinensis var mairei has the advantages that the liquid-to-solid ratio is reduced,the use of an organic solvent is reduced, the extraction time is shortened, the extraction efficiency is improved, and the problem of long production period of the 10-DAB extraction technology is fundamentally solved. The 10-DAB obtained by the preparation method disclosed by the invention is high in content and has practical promotion and application values.

The invention relates to a method for promoting growth of camptotheca acuminate. The method comprises the operating steps of obtaining a methyl alcohol extracting solution concentrate of branches and leaves of taxus chinensis var mairei, and irrigating the camptotheca acuminate with a water suspension of the methyl alcohol extracting solution concentrate. The method for promoting growth of the camptotheca acuminate is simple and high in operability, and by the adoption of the method, the whole plant biomass of the camptotheca acuminate can be improved by more than 18%, and the content of camptothecin in the camptotheca acuminate is increased by more than 12%.