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Method for promoting growth of camptotheca acuminate and increasing content of camptothecin

A technology of camptothecin and content, applied in the field of plant ecology and plant physiology research, can solve the problems of poor growth of pure camptothecin forest and small seed yield per plant, and achieve the effect of promoting the growth of camptotheca

Active Publication Date: 2014-06-04
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to alleviate the crisis of camphor tree resources, my country has begun to pay attention to the research on camphor tree plantation cultivation (Yang Ronghe, 2001; Mei Jilin and Wang Hui, 2006; Zeng Shuyan and Li Yingzhen, 2007; Wang Guibin et al., 2009; Liu Yu et al., 2011 ), the results of previous studies showed that the growth of camphor tree pure forest was poor, and the seed setting per plant was small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Follow the steps below:

[0012] (1) Dry and pulverize the branches and leaves of Taxus chinensis to obtain powder I, mix methanol and powder I at a mass ratio of 5:1, reflux and extract for 1 hour, filter the extract, concentrate the filtrate to dryness, and obtain solid II;

[0013] (2) Mix the solid substance II obtained in step (1) with water at a mass ratio of 1:1000, and perform ultrasonic treatment for 8 minutes to obtain a solid substance II suspension;

[0014] (3) Select philodendron seedlings with the same seedling age and consistent growth and divide them into 2 groups, and carry out soil culture under natural light in the greenhouse, with 5 pots in each group and 1 plant in each pot; wherein, the treatment group is watered with solid matter every 3 days. 100 mL of Ⅱ suspension; the control group was irrigated with 100 mL of distilled water; in addition to watering the solid Ⅱ suspension or distilled water, each group was naturally replenished with water dep...

Embodiment 2

[0017] (1) Dry and pulverize the branches and leaves of Taxus chinensis to obtain powder I, mix methanol and powder I at a mass ratio of 10:1, reflux and extract for 2 hours, filter the extract, concentrate the filtrate to dryness, and obtain solid II;

[0018] (2) Mix the solid substance II obtained in step (1) with water at a mass ratio of 1:100, and perform ultrasonic treatment for 10 minutes to obtain a solid substance II suspension;

[0019] (3) Select philodendron seedlings with the same seedling age and consistent growth and divide them into 2 groups, and carry out soil culture under natural light in the greenhouse, with 5 pots in each group and 1 plant in each pot; wherein, the treatment group is watered with solid matter every 3 days. 100 mL of Ⅱ suspension; the control group was irrigated with 100 mL of distilled water; in addition to watering the solid Ⅱ suspension or distilled water, each group was naturally replenished with water depending on the soil moisture; aft...

Embodiment 3

[0022] (1) Dry and crush the branches and leaves of Taxus chinensis to obtain powder I, mix methanol and powder I at a mass ratio of 8:1, reflux and extract for 3 hours, filter the extract, concentrate the filtrate to dryness, and obtain solid II;

[0023] (2) Mix the solid substance II obtained in step (1) with water at a mass ratio of 1:600, and perform ultrasonic treatment for 5 minutes to obtain a solid substance II suspension;

[0024] (3) Select philodendron seedlings with the same seedling age and consistent growth and divide them into 2 groups, and carry out soil culture under natural light in the greenhouse, with 5 pots in each group and 1 plant in each pot; wherein, the treatment group is watered with solid matter every 3 days. 100 mL of Ⅱ suspension; the control group was irrigated with 100 mL of distilled water; in addition to watering the solid Ⅱ suspension or distilled water, each group was naturally replenished with water depending on the soil moisture; after 60 ...

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Abstract

The invention relates to a method for promoting growth of camptotheca acuminate. The method comprises the operating steps of obtaining a methyl alcohol extracting solution concentrate of branches and leaves of taxus chinensis var mairei, and irrigating the camptotheca acuminate with a water suspension of the methyl alcohol extracting solution concentrate. The method for promoting growth of the camptotheca acuminate is simple and high in operability, and by the adoption of the method, the whole plant biomass of the camptotheca acuminate can be improved by more than 18%, and the content of camptothecin in the camptotheca acuminate is increased by more than 12%.

Description

technical field [0001] The invention belongs to the field of plant ecology and plant physiology research, and specifically relates to a method for promoting the growth of camptothecin and increasing the content of camptothecin. Background technique [0002] Campodia, aliases: Eclipta, water chestnut, water tung tree, Qianzhang tree, Latin scientific name: Camptotheca acuminata , is a fast-growing tree species in the genus Cymbidium and Campylodontaceae, and is a national second-level key protected wild plant. Camptophyllum is produced in southern Jiangsu, Zhejiang, Fujian, Jiangxi, Hubei, Hunan, Sichuan, Guizhou, Guangdong, Guangxi, Yunnan and other provinces and regions. Camptotheca has a straight trunk and grows rapidly. It can be used as a garden tree or a street tree. It is especially worth mentioning that the whole plant of Camptotheca contains an indole alkaloid—camptothecin, and the content of the fruit and leaves is the highest (Thomas et al., 2004). Camptotheci...

Claims

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Application Information

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IPC IPC(8): A01G7/06
Inventor 不公告发明人
Owner NORTHEAST FORESTRY UNIVERSITY
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