246 results about "Cymbidium" patented technology

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The invention provides a nutrition liquid of cymbidium hyridus, which is composed of N, P, K, Ca, Mg, Fe, B, Ma, Zn, Cu and Mo and etc with a proper proportion. The nutrition liquid can make the cymbidium hyridus grow faster and stronger and reduce the plant diseases and insect pests.

The invention provides a comprehensive prevention and control method for anthracnose of potted sword-leaved cymbidium, belonging to the field of agricultural plant disease control. By aiming at the four growth stages including a leaf-bud budding stage, a bud and young leave growth stage, a flowering stage and a dormancy recovery stage of the potted sword-leaved cymbidium, five prevention and control measures including agricultural prevention and control, plant quarantine, illness monitoring, biological prevention and control, and chemical agent prevention and control are integrally assembled, so that the comprehensive prevention and control measures suitable for each growth stage are provided. The comprehensive prevention and control method for the anthracnose of the potted sword-leaved cymbidium can efficiently control the anthracnose of the cymbidium, obviously reduces number of use and usage amount of pesticide, and has great significance for improving the ornamental value and the economic value of the potted sword-leaved cymbidium and protecting the ecologic environment.

The invention discloses a florescence control method for cymbidium hybridum, which comprises the following steps: 1. carrying out first flower-formation promoting starting from 180 days before a desired-to-flower date, lasting for 30 days, and applying a first hormone solution every 5 days, wherein the first hormone solution refers to a gibberellin GA3 solution or a 15% paclobutrazol PP333 solution, from the first time, the concentration of the first hormone solution is linearly increased by a span of 5-15 mg/L each time, and the concentration of the first hormone solution used for the first time is 10-50 mg/L; and 2. carrying out second flower-formation promoting starting from 30 days before the desired-to-flower date, lasting for 15 days, and applying a second hormone solution every 2 days, wherein the hormone solutes of the first hormone solution and the second hormone solution are the same, the concentration of the second hormone solution used for each time is the same, and the concentration is 3-7 mg/L. By using the method disclosed by the invention, the cymbidium hybridum can be in its flowering season 5-25 days ahead of schedule, and the flowering season can be in the same period as the Spring Festival; the florescence control range is larger, the control on florescence is more accurate, and the florescence control error is reduced to a range of minus or plus 2 days.

The invention relates to the field of plant genetic breeding technology, and particularly discloses a method for creating a cymbidium resource for high-efficiency generation of 2n male gametes. The method includes parent matching, distant hybridization combination, hybrid colony construction, 2n male gamete identification, screening of the resource for high-efficiency generation of the 2n male gametes and the like. With utilization of the distant hybridization method, the cymbidium resource for high-efficiency generation of the 2n male gametes is obtained, and lays the foundation for high-efficiency creation of sexual polyploids by utilizing a sexual hybridization method, effective promotion of cymbidium polyploidy breeding, and cultivation of a breakthrough new cymbidium species in the absence of polyploids.

The invention discloses a cymbidium culture method, which comprises the following steps that (1) the cymbidium is subjected to strain division, and each strain of the cymbidium at least comprises three seedlings; the cymbidium subjected to the strain division is subjected to root covering by gauze and is put into nutrition liquid to be soaked for 2 to 3 days; after being taken out, the seedlings are dried for use; (2) a cymbidium culture substrate is prepared from the following materials including vermiculite, leaf mold, plant ash, fly ash, feather meal, wormcast, waste and abandoned fruit peel, pine needles and slow release fertilizer and water-retaining agents; before 1 to 2 days of the cymbidium culture, 200 to 300 mL of carbendazol or potassium permanganate solutions are sprayed on each square meter of the culture substrate. The method has the advantages that after the strain division, the roots of the cymbidium are covered by the gauze and are put into the nutrition liquid to be soaked, so that the root infection due to cymbidium strain division can be reduced; the root growth capability of the cymbidium can be improved during the cymbidium strain planting through indoleacetic acid and gibberellin contained in the nutrition liquid; the cymbidium can fast generate root and germinate; the cymbidium survival rate is improved.

The invention provides a Chinese cymbidium pot culture substrate prepared from peanut shell or coconut bran and stones. The Chinese cymbidium pot culture substrate is prepared by fully fermenting the peanut shell or coconut bran, and mixing the fermented peanut shell or coconut bran with stones. The composite substrate provided by the invention can improve the physical and chemical properties of the substrate, reduce adverse reactions in single substrate, promote the growth of Chinese cymbidium, reduce production cost while assuring the commercial quality of the potted Chinese cymbidium, realize the recycling of the peanut shell or coconut bran which is agricultural or forestry waste, and create obvious social, economic, environmental and ecological benefit.

The invention provides a method of the aseptic seeding and culturing of the cymbidium aseptic seed, comprising the steps of aseptic seeding, aseptic seedling culturing and seedling hardening, which is characterized in that after the aseptic seeding of the seed, a low temperature and darkness pre-culture step is added. The invention aims at enabling the cymbidium aseptic seed to complete the after ripening process and to promote germination by using the low temperature and dark condition and simulating the proterozonic environment of the cymbidium aseptic seed. Compared with the prior art, the invention improves the average bourgeon rate of the aseptic seeding of the cymbidium aseptic seed by over 10 times and has the advantages of simpleness and practicality, short seed culturing period and convenience for a large-scale production and so on, and the invention lays a firm basis for the novel variety selection of the cymbidium aseptic crossbreed.

The invention relates to an industrial production method of Cymbidium germchit based on sexual hybridization. The method provided by the invention comprises the following steps of: firstly identifying chromosome number and ploidy of Cymbidium parents; selecting pairing hybridized combinations according to the ploidy; carrying out a sexual hybridization pollination of the Cymbidium; managing the pollinated Cymbidium; carrying out a nonsymbiotic germination culture on Cymbidium seeds; inducing and differentiating protocorms (PLBs) of the Cymbidium seeds; differentiating bigger seedlings of the Cymbidium; transplanting the Cymbidium seedlings into bottles; and managing the cultivation of the Cymbidium transplanted seedlings. On the basis of indentifying the chromosomes of the Cymbidium parents, the method provided by the invention selectively pairs the hybridized combinations and carries out the sexual hybridization pollination, so that the aims of improving varieties and breeding new varieties are achieved; then a lot of Cymbidium germchit can be industrially produced through a tissue culture method.

The invention discloses a peanut milk beverage and a processing method thereof. The peanut milk beverage is prepared from following raw materials in parts by weight: 20-30 parts of peanuts, 60-80 parts of milk, 6-8 parts of xylitol, 8-10 parts of apple juice, 2-3 parts of fritillaria cirrhosa, 1-3 parts of homalomena occulta, 2-4 parts of dendrobium nobile, 2-4 parts of curculigo orchioides, 1-2 parts of eucommia ulmoides, 2-3 parts of mangnolia officinalis, 2-4 parts of mango leaves, 1-3 parts of akebia trifoliata koidz, 1-2 parts of cymbidiums, 1-2 parts of madders and 5-8 parts of a nutrition additive. According to the peanut milk beverage and the processing method thereof, the method is simple to operate, the formula is reasonable, and the nutrition is abundant; various Chinese herbal medicinal ingredients which can be used for activating veins and activating collaterals, calming the liver and soothing the nerves, such as the fritillaria cirrhosa, the homalomena occulta, the dendrobium nobile, the curculigo orchioides and the like, so that the product has a very good health-care effect.

The invention discloses a method of sterile germination of seed and plant cultivating for thoroughwort. Collecting Cmbidium tracyanum L castle plants, C. iridioides plants and jasper orchid plants and cultivating them in the resource garden until the fruit of the orchid is 90% mature. The cultivating method compeises: culturing seeds in the modified culture medium by explant, trans-culturing when the seed grows sprout and root with root hair, rapid breeding and root-growing culturing, new root will grow out one month later. The traditional orchid breeding is primarily the single-plant propagation with a low propagation rate, the portion between old sprout and mew sprout is only about 1:1-3,and is hard for mass production. The invention applies modern biotechnology and breed millions of orchids only employing one sprout in one year, which can meet the orchid industrialization demand; sterilizing technique can also be proceeded and produce seeds free of virus, which deduces the production cost, increase the product quality and meet the orchid industrialization demand effectively.

The invention provides a Chinese cymbidium seedling breeding method. The method includes seedling hardening, namely picking rooted seedlings after transition for 2 to 3 weeks on the indoor natural light radiating condition, and cleaning culture medium clinging to the roots of the seedlings for preparation; seedling mycorrhization, namely coating the picked seedlings with matrix containing mycorrhiza fungi and planting into a concave disc, cultivating in a cool and well-ventilated place at the proper temperature, spraying water to the leaves to promote fresh root growth, moving the seedlings into a greenhouse to perform normal water and fertilizer management, and obtaining mycorrhization seedlings after October. The matrix includes, by weights: 1/3 part of peat, 1/3 part of dry humus soil and 1/3 part of laterite, and small amount of oak leaves third-stage fungi with bacteria liquid are added. The method has the advantages that the quality of Chinese cymbidium can be improved, and the healthy growth of the Chinese cymbidium can be guaranteed.

The invention discloses double-antibody sandwich ELSA regent box and the method for the Cymbidiummosaicvirus spot. The box is made up of the box body, every using liquid and the PVDF film. The preparation of the regent box is simple, quick, safe and reliable. The sample can be used little and it can be detected in a little time. Comparing to other serology detecting method, the method is simple, quick, sensitivity and high specialty, also it does not need the special device and get the result by the eye directly. So it can be used widely in the base unit.

The invention discloses a method for improving a preservation effect of cymbidium type protocorm. According to the invention, the cymbidium type protocorm is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the cymbidium type protocorm. The method specifically comprises the steps of pre-culture, contained liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g/L of carbon nano tubes. The method disclosed by the invention optimizes the preservation effect of the cymbidium type protocorm remarkably; as the carbon nano tubes are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.

The invention provides a molecular identification method for cymbidium varieties, which is a simple sequence repeat genome analysis-based molecular identification method for the cymbidium varieties and analyzes the genetic relation of the cymbidium varieties by optimizing a technical system of cymbidium simple sequence repeat (SSR) analysis. By directly detecting the DNA sequence difference of the variety with genetic stability and information diversity, the method can definitely distinguish and judge different varieties of cymbidium, and realize accurate and quick identification of the variety so as to provide a reliable technique for variety identification, seed and seedling market management, variety protection and elite seed breeding and the like. By optimizing the SSR technical system of cymbidium genome DNA, the molecular identification of the cymbidium varieties has high speed, short time and multiple polymorphisms, the problems of reality inspection and the like of the cymbidium seedlings can be solved before trading, and the loss of a producer or a buyer is avoided. The method can distinguish and identify all cymbidium varieties by using one and more effective primers, and realize DNA fingerprint identification of the cymbidium varieties.

The invention provides a cultivation method for cymbidium. The cultivation method for cymbidium comprises the steps of (1) preparing cultivation soil, wherein lava, coal ash, soil with rotten leaves, cymbidium soil, cymbidium stone and vermiculite are stirred, mixed evenly and sieved, and then the cultivation soil is prepared for use; (2) sowing seeds, wherein a clay pot with the caliber and the depth both being 20 cm is used as a cymbidium pot, the cultivation soil is placed in the cymbidium pot, and the cymbidium seeds are sown in the cultivation soil; (3) managing illumination, wherein the cymbidium pot is placed in a placed exposed to direct sunlight, a shading net is arranged in summer, shading starts at 10:30 am and ends at 16: 30 pm, and the shading net is removed at other time; (4) managing moisture, wherein watering is conducted once on the second day after the cultivation soil in the pot is completely dried every week, and the air humidity is kept between 30% to 40%; (5) managing fertilizer application, after cymbidium comes up, leaf fertilizer is sprayed three times every week, and in a small cymbidium seedling period, nitrogenous fertilizer is applied twice every week; (6) managing ventilation, wherein a ventilation quantity is kept through an industrial exhaust fan; (7) conducting overwinter management, wherein in winter, abscisic acid is sprayed every 10 days. By the adoption of the cultivation method for cymbidium, the quality of cymbidium can be effectively improved, and the abloom rate of cymbidium can be increased.

The invention discloses a seed germination and rapid propagation method for cymbidium orchid, and relates to the technical field of plant tissue culture. The method comprises the steps that culturing is conducted through a seed germination culture medium and then conducted through a protocorm seedling differentiation culture medium, wherein the seed germination culture medium is a VW culture medium containing 6-benzyl adenine with the concentration of 0.5 mg/L to 1.0 mg/L, and the protocorm seedling differentiation culture medium is a KC culture medium containing banana juice with the mass fraction of 10%. According to the method, the seed germination and propagation speed of the cymbidium orchid can be significantly increased, the method is especially suitable for Zijingxiangyun cymbidium orchid and Laoranzi varieties difficult to germinate and culture, the culture time can be shortened, the culture technology is simple, conditions are created for commercialized production of the cymbidium orchid, and the orchid market demand exceeding supply is met.

The invention discloses an immune colloidal gold test strip used for detecting cymbidium virus series and preparing method thereof, comprising an immune colloidal gold test strip used for detecting cymbidium CyMV virus and ORSV virus and preparing method thereof; the immune colloidal gold test strip used for detecting cymbidium virus series has the advantages of accurate result, fast determination, simple and convenient operation and low cost, etc., does not need washing process and standard contrast, can detect the samples in batches or singly, and is easy for promotion and use; the operator does not need professional training and can complete the operation according to the specification.

The invention discloses a herb tea for treating diabetes mellitus, and the herb tea comprises the following medicinal material components in parts by mass: 5-10 parts of Indian kalimeris herb, 5-10 parts of snokeweed, 15-20 parts of sword-leaved cymbidium, 10-15 parts of chinese ladiestresses root, 20-25 parts of nemo grass, 10-15 parts of trichosanthes kirilowii maxim and 20-25 parts of dried guava. The preparation method comprises the following steps of: getting the seven medicinal materials above, removing the impurities by selection, orderly putting the medicinal materials into a constant-temperature box for drying, then crushing the medicinal materials by a crusher, uniformly mixing the crushed materials according to the mass proportion, packing the crushed materials into bags made of filter paper by adopting a general teabag production technique, and then obtaining teabag herb tea, wherein each teabag contains 10 g of dried mixed herb tea powder. The herb tea is taken by a method as follows: brewing the herb tea by boiled water and drinking the herb tea as tea water. In 57 people with diabetes, who actually take the herb tea disclosed by the invention, the herb tea has a remarkable effect on 50 people and is effective on 3 people and ineffective on 4 people, so the herb tea disclosed by the invention is proved to have a remarkable curative effect.