24 results about "Cymbidium" patented technology

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The present application relates to the fields of molecular biology and plant breeding technology, and particularly relates to a primer pair for distinguishing between one-flowering and continuous-flowering Cymbidium varieties, application and kit thereof. The primer pair comprises FRI-M-F and FRI-M-R. The molecular marker FRI-M-F / FRI-M-R provided by the present application can accurately and efficiently distinguish between one-flowering Cymbidium varieties and continuous-flowering Cymbidium varieties, and can be used for molecular marker assisted screening and identification of the continuous-flowering trait of Cymbidium, thereby significantly improving the breeding efficiency.

The application discloses a Cymbidium cultivation method capable of controlling gradual color change and a novel color Cymbidium, and relates to the technical field of plant genetic engineering. The method comprises the following steps: constructing a fusion expression vector containing a P2A self-cleavage peptide, introducing the vector into Agrobacterium to obtain an engineered Agrobacterium, introducing the engineered Agrobacterium into a Cymbidium receptor material by using an Agrobacterium-mediated transformation method, and culturing, screening positive transformants by observing red pigment accumulation under environmental light, and adjusting transformation conditions to realize the synergistic accumulation or different expression levels of anthocyanins and betalains. The method realizes independent expression of double genes from a single mRNA, realizes device-free screening by combining RUBY visual marking, realizes color gradient control by transformation condition regulation, can realize stable coexistence of anthocyanins and betalains in the same tissue of Cymbidium, creates novel colors such as a purplish red color system or a gradual color, and shortens the transformation period to 5 days.

The application discloses a Cymbidium SSRR primer group and a method for constructing a Cymbidium variety fingerprint by using the primer group. The Cymbidium SSRR primer group comprises 14 pairs of SSR primer sequences. The 14 pairs of SSR primer sequences are used for carrying out multiplex PCR amplification on Cymbidium genomic DNA, the PCR products are detected by using capillary electrophoresis fluorescence, SSR marker data are analyzed, 70 kinds of Cymbidium SSR fingerprint maps are constructed, and Cymbidium varieties can be effectively identified. The method for constructing the Cymbidium variety fingerprint has the advantages of high throughput, high precision and low cost, and has a good application prospect.

The invention discloses a storage method and application of cymbidium seeds mixed with bactericidal sandy soil, and belongs to the technical field of agricultural product storage. According to the method, mixed and sterilized sandy soil and cymbidium orchid seeds are alternately treated at two temperature intervals of 2-6 DEG C and 20-30 DEG C, so that seed aging is delayed, and seed vitality, storage stability and germination rate are improved. Meanwhile, mature cymbidium capsules are collected and dried in the shade till the water content of the seeds is smaller than 10%, river sand and matrix soil are mixed and sterilized to serve as a storage medium, the air permeability and the water-retaining property are guaranteed, and the infection risk is reduced; a proper humidity environment is kept by spraying a mixed sterilizing solution, so that the storage effect is further improved; and the effectiveness and the controllability of the storage process are ensured by monitoring the temperature and the humidity in real time. The method is applied to artificial breeding of cymbidium orchid and storage of orchid seeds, the germination rate and the seedling success rate are increased, the genetic stability is kept, and powerful support is provided for production, research and application of cymbidium orchid and orchid.

The invention relates to the field of cosmetics and biotechnology thereof, in particular to a vesicle composition with a corn germ extract wrapped by black orchid cells as well as a preparation method and application of the vesicle composition. The vesicle composition disclosed by the invention is obtained by feeding a corn germ extract into a fermentation system of black orchid cells. The culture conditions of the fermentation system of the black orchid cells are as follows: the temperature is 22-25 DEG C, the speed is 120-180 rpm, and the culture period is 15-20 days. The corn germ extract can be added in a delay phase, an exponential phase, a later exponential phase and a plateau phase of fermentation culture of black cymbidium cells. The black cymbidium cell vesicle composition obtained by the invention has the advantages of low immunogenicity, enhanced cellular uptake, higher stability and targeting ability, and better affinity with a phospholipid bilayer of a skin cuticle. And after reaching the cuticle of the skin, active matters can be quickly released, so that the effects are achieved. The compound can be used for preparing antioxidant or anti-aging medicines or cosmetics.

The invention relates to a preparation method and application of a hemerocallis citrina mixed polypeptide oral liquid for relieving depression, the protein content of hemerocallis citrina is relatively high, 100g of dried hemerocallis citrina contains about 20g of protein, and in addition, the hemerocallis citrina is rich in amino acid composition, is an excellent plant protein polypeptide source, and can be used for preparing the oral liquid. The hemerocallis citrina mixed active polypeptide is prepared by obtaining hemerocallis citrina crude protein through an alkali extraction and acid precipitation method and then carrying out an enzymolysis process by combining trypsin, pepsin and intestinal protease, and experimental results show that the hemerocallis citrina mixed active polypeptide oral liquid has a good effect of preventing and treating depression, and the hemerocallis citrina as a common vegetable food has a wide application prospect. The tea is suitable for people of all ages and has good safety. The traditional Chinese medicine composition has a very good anti-depression effect, the edible value of the day lily can be improved, the income of farmers is increased, and a contribution is made to develop a novel agricultural industry.

This invention provides a method for screening and grading quality indicators for Rhododendron seedlings, thereby determining grading standards and classifying seedling quality. Through systematic testing of various quality indicators of one-year-old Rhododendron seedlings, and by comprehensively applying multivariate statistical analysis, principal component analysis was used to extract the three key indicators with the highest contribution, serving as the main basis for seedling grading. Combined with K-means clustering algorithm, seedlings were scientifically divided into four quality grades based on these core indicators. Verification has shown that these grading indicators can effectively reflect the growth of Rhododendron seedlings after transplanting, providing a scientific basis for quality control of Rhododendron seedlings in production.

The present invention discloses a method for simultaneously determining multiple chemical components in Cymbidium heterophyllum, and relates to the technical field of separation and analysis of natural products. Ultra-high performance liquid chromatography is used to simultaneously determine the chemical components of Cymbidium heterophyllum, and the chemical components include at least neochlorogenic acid, cryptochlorogenic acid, and diosgenin-7-O-glucuronide; the detection conditions of ultra-high performance liquid chromatography include: chromatographic column stationary phase: diisobutyl group is bonded to the silica gel surface through siloxane; mobile phase A is selected from methanol or acid-containing methanol, and mobile phase B is selected from water or acid-containing aqueous solution; mobile phase A and mobile phase B are used as a mixed mobile phase for gradient elution. The present invention establishes a UPLC content determination method for multiple chemical components in Cymbidium heterophyllum, which is simple to operate, has reliable results, and good repeatability, and can provide a certain reference for the determination of the content of chemical components in Cymbidium heterophyllum and the improvement of existing quality standards.

The invention belongs to the technical field of plant species identification, and relates to a primer, a detection method and a kit for identifying cymbidium turcicum through enzyme digestion and application of the primer, the detection primer is composed of a nucleotide sequence shown in SEQ ID NO.1 and a nucleotide sequence shown in SEQ ID NO.2, genome DNA of cymbidium turcicum serves as a template, PCR amplification is conducted through the primer, an amplification product is subjected to enzyme digestion through restriction endonuclease EcoRV, and the amplification product is subjected to PCR amplification through enzyme digestion. After the enzyme digestion product is subjected to agarose gel electrophoresis, two bands of 265bp and 216bp are obtained, so that the cymbidium sibiricum can be quickly identified according to the number and the size of the electrophoretic bands. The method disclosed by the invention is high in specificity and visual in identification result, and realizes rapid and accurate detection and identification of the cymbidium turcicum.

The invention discloses a direct seeding and seedling raising composite biological matrix for cremastra appendiculata and a use method of the composite biological matrix. The matrix is prepared from orthopaedic saw dust, a plant material compound, a composite functional fungicide and a mineral additive, the plant material compound is prepared from vinasse, furfural residues, turfy soil or / and medicine residues and waste lentinus edodes fungus bag residues; the effective viable count of the composite functional microbial inoculum in the composite biological matrix is greater than or equal to 5 billion CFU / g, and the composite functional microbial inoculum is formed by mixing bacillus subtilis, brevibacillus laterosporus and trichoderma harzianum according to the mass ratio of (2-4): (2-4): (3-5); the mineral additive is formed by mixing perlite and vermiculite according to the ratio of 1: 1. The composite biological substrate realizes combination of physical and chemical properties, biological prevention and control and nutrition supply through raw material synergy and process optimization, is suitable for the fields of agricultural traditional Chinese medicinal material seedling substrates, culture substrates, bio-organic fertilizer carriers and the like, improves the use effect through optimization of a traditional Chinese medicinal material rhododendron fargesii planting method, and is suitable for large-scale popularization and application in the fields of agricultural traditional Chinese medicinal material seedling substrates, culture substrates, bio-organic fertilizer carriers and the like. And expected social and economic benefits are achieved.

The invention provides a method for regulating and controlling soilless low-temperature vernalization and arrow drawing of cymbidium goeringii, and belongs to the technical field of cymbidium goeringii flowering phase regulation and control. The method comprises the following steps: (1) washing the root system of cymbidium goeringii, and airing to separate out plants with flower buds; (2) loading the plant root with the flower bud into a container containing a culture medium; (3) wrapping with a buffer product; (4) placing the plants in a refrigeration house to complete vernalization; and (5) spraying a plant growth regulator, wrapping the buffer product, and then transferring into an environment with the day temperature of 23-27 DEG C and the night temperature of 13-17 DEG C for flower forcing culture, so that the plant is subjected to arrow drawing, namely the vernalization and arrow drawing process of cymbidium goeringii is completed. According to the method for soilless low-temperature vernalization of the cymbidium goeringii and growth promotion of the arrows, the flowering period of the cymbidium goeringii is manually regulated and controlled, out-of-season or timed flowering of the cymbidium goeringii is achieved, the production cost is reduced, the efficiency is improved, and meanwhile the commodity value and market adaptability of the cymbidium goeringii are improved.

The present disclosure provides a plant composition comprising a plant extract, wherein the plant extract is present in the plant composition in an amount effective to produce a beneficial effect on intestinal health of an animal. The benefits may include one or more effects on the intestinal health of the animal, which may include one or more improvements in the intestinal health and immune health of the animal. The plant extracts can comprise dandelion leaf extracts, dandelion leaves, ground dandelion leaves, dandelion roots, panax japonicus, ferula asafetida, ligustrum lucidum, fennel, Indian mulberries, quispualis indica, capsicum annuum, Indian nelumbo nucifera, red peppers / capsicum, shiitake mushroom, custard apples, hericium erinaceus, caraway, saw palmetto, phoenix-tail cymbidium, mountain lily yucca, carob beans, wild lupin, goat heads, sweet rosa roxburghii tratt, damnacanthus indicus and herba lycopi; , Amaranthus spinosus, and Sambucus williamsii, or any combination thereof. Other aspects are also provided herein.

The invention discloses a method for inducing an intermediate propagule of cymbidium faberi, and belongs to the technical field of rapid propagation of orchid plants. Aiming at the problems that the cymbidium faberi is low in division reproduction rate, carries viruses and the like, the method comprises the following steps: culturing a rare line art cymbidium faberi seedling serving as an explant in a proper culture medium, and directly inducing an intermediate propagule. By utilizing the technology, the intermediate propagules can be obtained in batches in a short time, and a large batch of cymbidium faberi adult seedlings with stable hereditary characters can be rapidly obtained after differentiation culture, so that technical support is provided for large-scale breeding of the rare cymbidium faberi.

The present invention relates to a culture medium and method for promoting rapid seedling formation of Tiangui Cymbidium orchid seeds, and relates to the fields of plant biotechnology and plant cultivation technology. The culture medium includes a protocorm induction culture medium, a cluster bud differentiation culture medium, a cluster bud proliferation culture medium, and a rooting and seedling strengthening culture medium; the culture method includes the following specific steps: (1) sterile sowing and protocorm induction; (2) cluster bud differentiation culture; (3) cluster bud proliferation culture; and (4) cluster bud rooting and seedling strengthening culture. The present invention uses seeds of Tiangui Cymbidium orchid from different plants and flowers for cross-pollination as explants to construct a sterile sowing and culture system, which promotes rapid seed germination and a high germination rate, overcomes the disadvantages of using bulbs or tender shoots as explants, such as waste of materials and low induction rate, while improving production efficiency and reducing production costs.

The utility model discloses a Chinese orchid potting fertilizing device which comprises a box body, a support is fixedly connected to the top of the front face of the box body, a first motor is fixedly connected to the middle of the upper surface of the support, and the output end of the first motor is fixedly connected with a lead screw. According to the fertilizing device for Chinese orchid potting, through the arrangement of a lead screw, supporting pipes, a conveying pipe, a sharp corner and a through hole, the output end of a first motor drives the lead screw to rotate, a sliding plate moves up and down, the height of the conveying pipe is adjusted, fertilizer water can be directly conveyed to the root of Chinese orchid, the two sets of supporting pipes can rotate by a certain angle, and the Chinese orchid is prevented from being damaged during fertilizing; a plurality of conveying pipes convey fertilizer water to the root of the Chinese orchid through a plurality of through holes in the bottom, so that the Chinese orchid can better absorb water and nutrition, the fertilization uniformity is ensured, the fertilization efficiency is further improved, gaps between soil are enlarged through sharp corners, and air easily enters the soil, so that the respiration of root cells of the Chinese orchid is increased, and the survival rate of the Chinese orchid is increased. The root system absorption and growth are promoted.

The invention discloses a coprinus comatus strain for promoting seed germination of cremastra appendiculata and application of the coprinus comatus strain, the strain is separated and screened from a soil sample collected within the depth range of 0-10 cm around the root system of cremastra appendiculata, the strain is identified as coprinus comatus through ITS sequencing, the Latin name is Coprinus saccharinus, and the preservation number is CGMCC No.42052. The invention further discloses a preparation method of the coprinus comatus strain for promoting seed germination of cremastra appendiculata and application of the coprinus comatus strain for promoting seed germination of cremastra appendiculata. The coprinus comatus strain and the rhododendron simsii seeds are subjected to symbiotic germination culture, the germination rate of the seeds can reach 60%-70%, the difference of the germination rates of different batches is small, and the stability is high; meanwhile, the coprinus comatus strain can quickly establish a symbiotic relationship with the rhododendron seeds to provide timely nutritional support for seed germination, so that the seeds can begin to germinate 15-20 days after being sown, the germination time is shortened, the breeding efficiency is improved, the coprinus comatus strain has very high value in rhododendron plant seedling culture, the market demand can be quickly met, and the coprinus comatus strain can be widely applied to seedling culture of rhododendron plants. Good economic benefits are obtained, the problem of seedling shortage of the cremastra appendiculata is solved, and the key problem of promotion of standardized breeding and cultivation of the cremastra appendiculata is solved.

1. Name of the designed product: building blocks (Cymbidium). 2. Use of the designed product: for toys. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model relates to the technical field of orchid cultivation, in particular to a Chinese orchid cultivation device which comprises an H-shaped base, a detachable bottom basin is movably installed on the base, a plurality of water storage grooves and a plurality of ventilation grooves distributed with the water storage grooves in a spaced mode are formed in the top side of the bottom basin, an outer basin is rotationally connected into the bottom basin, and the outer basin is provided with a plurality of water storage grooves and a plurality of ventilation grooves distributed with the water storage grooves in a spaced mode. A plurality of uniformly distributed first air holes are formed in the bottom end of the outer pot, and an inner pot is rotationally connected to the interior of the outer pot. The flowerpot has the advantages that the outer flowerpot body and the inner flowerpot body are arranged in a rotatable mode, redundant water can be discharged into the water storage tank to be stored when a large amount of water is encountered, and external air can enter the inner flowerpot body through the first ventilation hole, the second ventilation hole and the ventilation groove; the soil in the inner pot can exchange gas with the outside, the situation that soft roots of Chinese orchid are rotted due to the fact that the soil humidity in the inner pot is large is avoided, and normal growth of the Chinese orchid is guaranteed.

This invention provides a *Ceratophyllum demersum* strain and its applications. The *Ceratophyllum demersum* strain has the accession number CCTCC NO: M20252398 at the China Center for Type Culture Collection, with a deposit date of October 30, 2025. The *Ceratophyllum demersum* strain of this invention can synergistically promote the multi-stage development of *Cymbidium* without requiring the replacement of different fungi, simplifying the propagation process; it reduces competition between fungal species and symbiotic instability caused by environmental factors, improving the consistency of germination rate and seedling survival rate. It eliminates the need for multi-strain staged inoculation and culture management, reducing operational steps and costs, making it suitable for greenhouse seedling cultivation and large-scale propagation.

The invention provides a tea processing device and an orchid tea processing system, and belongs to the technical field of orchid tea production. The tea processing device comprises a rolling mechanism and an air supply mechanism. A containing groove is formed in the inner ring wall of a rolling disc of the rolling mechanism, the inner diameter of a matching ring is smaller than that of the rolling disc, and the matching ring is matched in the containing groove. The outer side wall of a rolling barrel of the rolling mechanism is simultaneously attached to the inner ring wall of the matching ring and the inner ring wall of the rolling disc, so that the inner ring wall of the matching ring is flush with the inner ring wall of the rolling disc at the position attached to the rolling barrel, and the inner ring wall of the matching ring is located on the side, close to the central axis of the rolling disc, of the inner ring wall of the rolling disc at other positions. The air supply mechanism communicates with the containing groove. The tea leaf rolling device can further improve the tea leaf rolling sufficiency, prevents the tea leaves from remaining at the edge of the rolling disc, improves the rolling output rate, and reduces the cleaning workload at the same time.