41 results about "Cymbidium" patented technology

The invention discloses a Chinese orchid flowering regulation gene CsFTL3 as well as an encoding protein and application thereof, and belongs to the field of plant molecular biology. The gene CsFTL3 for regulating and controlling flowering of Chinese cymbidium is separated from flower bud cDNA (complementary deoxyribonucleic acid) of a Chinese cymbidium variety 'Xiaoxiang', and 174 amino acids are encoded by the gene CsFTL3. Through construction of an expression vector of the CsFTL3 gene, overexpression in wild-type arabidopsis thaliana and heterologous overexpression of the CsFTL3 gene in the arabidopsis thaliana, it is found that bolting and flowering periods of the arabidopsis thaliana are significantly delayed, flower stems are significantly thickened and increased, the number of rosette leaves is significantly increased, and the growth period is prolonged, which indicates that the gene has the function of delaying the flowering time of the arabidopsis thaliana. The Chinese orchid flowering regulation and control gene can be used for molecular mechanism research of orchid flowering time, delay Chinese orchid flowering time, promote plant vegetative growth, prolong whole Chinese orchid viewing time and promote Chinese orchid genetic improvement and variety breeding.

The invention provides a method for sterile sowing and efficient propagation of cymbidium faberi. The method comprises the following steps: pretreatment of cymbidium faberi seeds, sterile germination of the cymbidium faberi seeds, protocorm proliferation, differentiation, seedling strengthening and acclimatization and transplantation. The pretreatment comprises the steps of refrigerating hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with sodium hypochlorite, performing dark culture for 10-30 days after sowing, and then performing light culture, and the germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl aminopurine, 0.1-0.5 mg / L of naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of activated carbon. According to the method, the seed germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the pollution rate is reduced to 5% or below, 50.0 g / L inositol, 2.0 mg / L glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the growth time of cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium resource protection and new variety cultivation.

The artificial breeding method comprises the following steps: parent fish culture: selecting wild cymbidium for cage culture, and feeding culture bait; after the weight of the parent fishes reaches the standard that the weight of the female fishes is larger than 200 g / fish and the weight of the male fishes is larger than 150 g / fish, round fishes which are robust in physique, lively in action, high in stress resistance and free of body surface trauma are selected as the parent fishes, the parent fishes are transferred into the parent fish pond from the net cage, and the proportion of the female parent fishes to the male parent fishes is (2-2.5): 1. According to the method disclosed by the invention, the fortified bait prepared from the iced trash fish, the eel meal, the probiotic, the multivitamins, the fish oil, the vitality-promoting agent and the yeast is used for fortifying the parent fish of the Cymbidium, and a certain dosage of HCG is used for carrying out ripening and spawning induction treatment on the female parent and the male parent, so that the Cymbidium fry can be obtained on a large scale; therefore, the artificial breeding of the cynanchum otophyllum fries is realized, the blank of the artificial breeding technology of the cynanchum otophyllum is filled, a foundation is provided for the large-scale production of the cynanchum otophyllum fries, and the popularization of

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The present application relates to the fields of molecular biology and plant breeding technology, and particularly relates to a primer pair for distinguishing between one-flowering and continuous-flowering Cymbidium varieties, application and kit thereof. The primer pair comprises FRI-M-F and FRI-M-R. The molecular marker FRI-M-F / FRI-M-R provided by the present application can accurately and efficiently distinguish between one-flowering Cymbidium varieties and continuous-flowering Cymbidium varieties, and can be used for molecular marker assisted screening and identification of the continuous-flowering trait of Cymbidium, thereby significantly improving the breeding efficiency.

The invention relates to a preservation method of a Cymbidium macrocephalum protocorm. The preservation method comprises the following steps: obtaining the Cymbidium macrocephalum protocorm; soaking the protocorm into a loading solution for loading treatment; soaking the loaded material into a vitrification solution for vitrification treatment; wherein the vitrification solution is a PVS2 solution; the soaking time is 0-3 hours; and storing the vitrified protocorm at an ultralow temperature. The protocorm treatment method is simple to operate, and the cost can be greatly saved. And the survival rate of the protocorm preserved by the method provided by the invention is high.

The application discloses a Cymbidium cultivation method capable of controlling gradual color change and a novel color Cymbidium, and relates to the technical field of plant genetic engineering. The method comprises the following steps: constructing a fusion expression vector containing a P2A self-cleavage peptide, introducing the vector into Agrobacterium to obtain an engineered Agrobacterium, introducing the engineered Agrobacterium into a Cymbidium receptor material by using an Agrobacterium-mediated transformation method, and culturing, screening positive transformants by observing red pigment accumulation under environmental light, and adjusting transformation conditions to realize the synergistic accumulation or different expression levels of anthocyanins and betalains. The method realizes independent expression of double genes from a single mRNA, realizes device-free screening by combining RUBY visual marking, realizes color gradient control by transformation condition regulation, can realize stable coexistence of anthocyanins and betalains in the same tissue of Cymbidium, creates novel colors such as a purplish red color system or a gradual color, and shortens the transformation period to 5 days.

The invention discloses a cymbidium plant seedling transplanter, and particularly relates to the technical field of plant cultivation, the cymbidium plant seedling transplanter comprises a mounting plate, a bearing plate and a motor I. A mounting block is fixed to one end of the mounting plate, a sliding rail is fixed to one end of the mounting block, a sliding block is slidably connected to one end of the sliding rail, and an L-shaped plate is arranged at one end of the sliding block through a spring; a waved plate matched with the L-shaped plate is fixed to the vertical portion of the L-shaped plate and one end of the mounting block, a first air cylinder is fixed to the horizontal portion of the L-shaped plate, a clamping component is fixed to the output end of the first air cylinder, and a placing table is fixed to the upper end of the bearing plate. According to the cymbidium plant seedling transplanting machine, original hilling soil of cymbidium seedlings can be removed in the cymbidium seedling transplanting process through the L-shaped plate, the waved plate, the sliding block, the first air cylinder and the clamping component, disease and pest propagation can be better avoided, the soil environment is improved, and fusion of root systems and new soil is promoted; and the orchid can better grow and develop in a new environment.

The application discloses a Cymbidium SSRR primer group and a method for constructing a Cymbidium variety fingerprint by using the primer group. The Cymbidium SSRR primer group comprises 14 pairs of SSR primer sequences. The 14 pairs of SSR primer sequences are used for carrying out multiplex PCR amplification on Cymbidium genomic DNA, the PCR products are detected by using capillary electrophoresis fluorescence, SSR marker data are analyzed, 70 kinds of Cymbidium SSR fingerprint maps are constructed, and Cymbidium varieties can be effectively identified. The method for constructing the Cymbidium variety fingerprint has the advantages of high throughput, high precision and low cost, and has a good application prospect.

The utility model discloses a drone for Chinese milk vetch sowing, comprising a drone main body and an anti-blocking intermittent material guiding device. The utility model is provided with an anti-blocking intermittent material guiding device, that is, the intermittent receiving and transmission of Chinese milk vetch seeds can be realized under the cooperation of the limiting frame, the turntable and the receiving groove provided inside the intermittent component to intermittently rotate and receive materials, thereby reducing the occurrence of the phenomenon of blockage of the discharging of Chinese milk vetch seeds, and a transmission component is provided, that is, through the meshing transmission of the first bevel gear and the second bevel gear, a feeding rod with a spiral strip can be quickly rotated to transmit seeds for dispersed sowing cooperation, and a sowing component is provided, that is, the feeding rod can synchronously drive the main bevel gear to rotate, thereby indirectly realizing the transmission of the driving wheel, the belt and the driven wheel, thereby, the feeding plate can rotate and cooperate with the guide port opened on the outside to assist the rotation sowing cooperation, thereby achieving the advantages of the auxiliary centrifugal rotation sowing cooperation of Chinese milk vetch seeds.

The invention discloses a storage method and application of cymbidium seeds mixed with bactericidal sandy soil, and belongs to the technical field of agricultural product storage. According to the method, mixed and sterilized sandy soil and cymbidium orchid seeds are alternately treated at two temperature intervals of 2-6 DEG C and 20-30 DEG C, so that seed aging is delayed, and seed vitality, storage stability and germination rate are improved. Meanwhile, mature cymbidium capsules are collected and dried in the shade till the water content of the seeds is smaller than 10%, river sand and matrix soil are mixed and sterilized to serve as a storage medium, the air permeability and the water-retaining property are guaranteed, and the infection risk is reduced; a proper humidity environment is kept by spraying a mixed sterilizing solution, so that the storage effect is further improved; and the effectiveness and the controllability of the storage process are ensured by monitoring the temperature and the humidity in real time. The method is applied to artificial breeding of cymbidium orchid and storage of orchid seeds, the germination rate and the seedling success rate are increased, the genetic stability is kept, and powerful support is provided for production, research and application of cymbidium orchid and orchid.

The invention relates to the field of cosmetics and biotechnology thereof, in particular to a vesicle composition with a corn germ extract wrapped by black orchid cells as well as a preparation method and application of the vesicle composition. The vesicle composition disclosed by the invention is obtained by feeding a corn germ extract into a fermentation system of black orchid cells. The culture conditions of the fermentation system of the black orchid cells are as follows: the temperature is 22-25 DEG C, the speed is 120-180 rpm, and the culture period is 15-20 days. The corn germ extract can be added in a delay phase, an exponential phase, a later exponential phase and a plateau phase of fermentation culture of black cymbidium cells. The black cymbidium cell vesicle composition obtained by the invention has the advantages of low immunogenicity, enhanced cellular uptake, higher stability and targeting ability, and better affinity with a phospholipid bilayer of a skin cuticle. And after reaching the cuticle of the skin, active matters can be quickly released, so that the effects are achieved. The compound can be used for preparing antioxidant or anti-aging medicines or cosmetics.

The present invention provides a method for inducing the rhizomes of spring orchid test tube seedlings to bloom. It belongs to the field of plant tissue culture technology, and the specific steps are: taking out the seeds and inoculating them in a germination medium and culturing them for 2-10 days to obtain rhizomes; then placing the rhizomes in a proliferation medium and culturing them into robust rhizomes, and then inoculating the robust rhizomes into a flower bud induction medium to induce the formation of flower buds, and finally inoculating the flower buds into a flowering medium, culturing for 20 days until the flower buds are obviously enlarged and form a complete flower. The method adopted by the present invention directly promotes the rhizomes of spring orchid test tube seedlings to transition from the vegetative growth stage to the reproductive growth stage, induces flowering, determines the shape of hybrid offspring in advance, greatly shortens the traditional spring orchid breeding cycle, and improves the induction rate and flowering rate of flower buds by regulating the concentration of each component in the flower bud induction medium and the flowering medium.

The invention relates to a preparation method and application of a hemerocallis citrina mixed polypeptide oral liquid for relieving depression, the protein content of hemerocallis citrina is relatively high, 100g of dried hemerocallis citrina contains about 20g of protein, and in addition, the hemerocallis citrina is rich in amino acid composition, is an excellent plant protein polypeptide source, and can be used for preparing the oral liquid. The hemerocallis citrina mixed active polypeptide is prepared by obtaining hemerocallis citrina crude protein through an alkali extraction and acid precipitation method and then carrying out an enzymolysis process by combining trypsin, pepsin and intestinal protease, and experimental results show that the hemerocallis citrina mixed active polypeptide oral liquid has a good effect of preventing and treating depression, and the hemerocallis citrina as a common vegetable food has a wide application prospect. The tea is suitable for people of all ages and has good safety. The traditional Chinese medicine composition has a very good anti-depression effect, the edible value of the day lily can be improved, the income of farmers is increased, and a contribution is made to develop a novel agricultural industry.

The invention discloses a preparation and suspension culture method of black orchid callus cells, and belongs to the technical field of industrial biology. The method comprises the following steps that after the black orchid is disinfected, stamens are taken and inoculated into an induction culture medium with the pH value being 5-7, induction culture is conducted for 15-21 days under the condition that the temperature is 20-30 DEG C and no light exists, the black orchid callus is obtained, and the induction culture medium is composed of an MS basic salt culture medium, 25-35 g / L of saccharose, 6-7 g / L of agar, 1.0-2 mg / L of 6-BA, 0.5-1.5 mg / L of NAA, 0.3-1.0 mg / L of 2, 4-D and 0.5-1.5 g / L of coconut water. According to the method, the callus yield of the callus is 88.6%, the induction rate of the embryogenic callus capable of being cultured in a suspension manner is 68.6%-91.8%, and the callus with a good state is provided for large-scale culture of black cymbidium cells.

This invention provides a method for screening and grading quality indicators for Rhododendron seedlings, thereby determining grading standards and classifying seedling quality. Through systematic testing of various quality indicators of one-year-old Rhododendron seedlings, and by comprehensively applying multivariate statistical analysis, principal component analysis was used to extract the three key indicators with the highest contribution, serving as the main basis for seedling grading. Combined with K-means clustering algorithm, seedlings were scientifically divided into four quality grades based on these core indicators. Verification has shown that these grading indicators can effectively reflect the growth of Rhododendron seedlings after transplanting, providing a scientific basis for quality control of Rhododendron seedlings in production.

The utility model is suitable for the technical field of cymbidium hybridum planting, and provides a cymbidium hybridum inclined-top small-arch inner shed device which comprises a plurality of supporting columns. The outer shed is arranged on the plurality of supporting columns; the frame is arranged on the plurality of supporting columns; the plurality of inner sheds are arranged on the frame; the plurality of V-shaped water collecting tanks are respectively and fixedly arranged on the plurality of supporting columns; the two triangular water collecting tanks are mounted on the inner walls of the two sides of the outer shed respectively; the plurality of water tanks are respectively arranged on one sides of the plurality of supporting columns; the plurality of water spray irrigation mechanisms are mounted at the bottom of the frame and are used for performing water spray irrigation on the cymbidium hybridum. The inclined-top small-arch inner greenhouse device for cymbidium hybridum provided by the scheme has a condensed water collecting function, can prevent the condensed water from continuously dripping on leaf surfaces of the cymbidium hybridum along a greenhouse film at the top of the greenhouse, reduces the occurrence of plant diseases and insect pests, reduces the use amount of pesticides, and has an auxiliary water spraying irrigation function.

The present invention discloses a method for simultaneously determining multiple chemical components in Cymbidium heterophyllum, and relates to the technical field of separation and analysis of natural products. Ultra-high performance liquid chromatography is used to simultaneously determine the chemical components of Cymbidium heterophyllum, and the chemical components include at least neochlorogenic acid, cryptochlorogenic acid, and diosgenin-7-O-glucuronide; the detection conditions of ultra-high performance liquid chromatography include: chromatographic column stationary phase: diisobutyl group is bonded to the silica gel surface through siloxane; mobile phase A is selected from methanol or acid-containing methanol, and mobile phase B is selected from water or acid-containing aqueous solution; mobile phase A and mobile phase B are used as a mixed mobile phase for gradient elution. The present invention establishes a UPLC content determination method for multiple chemical components in Cymbidium heterophyllum, which is simple to operate, has reliable results, and good repeatability, and can provide a certain reference for the determination of the content of chemical components in Cymbidium heterophyllum and the improvement of existing quality standards.

The invention discloses a comprehensive treatment method for jujube witches broom of jujube trees, and relates to the technical field of agriculture. The method comprises the following steps: pruning diseased branches; applying stellera chamaejasme extract to the root soil of the jujube tree with the branches trimmed off; a diluent of a treatment agent B is sprayed on leaf surfaces of jujube witches broom plants, and the jujube witches broom treatment agent B is obtained in the mode that ginkgo leaves, astragalus sinicus and stellera chamaejasme serve as raw materials and are subjected to ethanol reflux extraction, an obtained crude extract is dissolved in tert-butyl alcohol, and 60 Co-gamma irradiation is conducted. According to the method, a comprehensive treatment means is adopted for the jujube witches broom, on the basis that diseased branches are pruned and removed through a physical means, the roots and leaves of jujube witches broom plants are synchronously treated through different treatment agents, pathogenic substances of the jujube witches broom and pests serving as virus transmission media are effectively killed, and then the jujube witches broom of jujube trees is effectively controlled.

The present invention discloses a spring orchid CgARF2 gene and its application in regulating leaf growth and development. The nucleotide sequence of the CgARF2 gene is shown in SEQ ID NO. 1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO. 2. The present invention cloned the CgARF2 gene sequence from the spring orchid cultivar "Songmei" and performed expression analysis in the spring orchid. Subsequently, the gene sequence was constructed into an overexpression vector and introduced into target plants to verify its function. It was found that Arabidopsis plants overexpressing the CgARF2 gene showed phenotypes such as an increased number of rosette leaves, smaller leaves, and curled leaf margins compared to wild-type plants. The rooting rate and root length of detached leaves on MS medium containing IAA were increased, and the content of endogenous hormones IAA and GA in the leaves was increased, while the content of ABA, MeJA, and BR was reduced. It can be seen that the gene will have a wide range of uses in the cultivation and trait improvement of orchids and other horticultural plants.

The invention belongs to the technical field of plant species identification, and relates to a primer, a detection method and a kit for identifying cymbidium turcicum through enzyme digestion and application of the primer, the detection primer is composed of a nucleotide sequence shown in SEQ ID NO.1 and a nucleotide sequence shown in SEQ ID NO.2, genome DNA of cymbidium turcicum serves as a template, PCR amplification is conducted through the primer, an amplification product is subjected to enzyme digestion through restriction endonuclease EcoRV, and the amplification product is subjected to PCR amplification through enzyme digestion. After the enzyme digestion product is subjected to agarose gel electrophoresis, two bands of 265bp and 216bp are obtained, so that the cymbidium sibiricum can be quickly identified according to the number and the size of the electrophoretic bands. The method disclosed by the invention is high in specificity and visual in identification result, and realizes rapid and accurate detection and identification of the cymbidium turcicum.

The invention relates to the technical field of tissue culture seedling transplanting, in particular to a Chinese orchid tissue culture seedling transplanting device which comprises a conveying mechanism, a first mounting frame, a second mounting frame, a third mounting frame, a planting frame, a movable lifting mechanism and a moving-out mechanism. The movable lifting mechanism is arranged at the top end of the first mounting frame. According to the Chinese orchid tissue culture seedling transplanting device, mechanical damage to roots and bases of Chinese orchid tissue culture seedlings can be avoided, meanwhile, automatic transplanting of the Chinese orchid tissue culture seedlings is achieved, the survival rate of the transplanted Chinese orchid tissue culture seedlings is effectively guaranteed, the transplanting efficiency of the Chinese orchid tissue culture seedlings is improved, and after the transplanted Chinese orchid tissue culture seedlings are cleaned, the transplanting efficiency of the Chinese orchid tissue culture seedlings is improved. According to the Chinese orchid tissue culture seedling screening device, automatic screening of Chinese orchid tissue culture seedlings is achieved, abnormal tissue culture seedlings and tissue culture seedlings with unqualified root development are separated to the second screen, meanwhile, healthy tissue culture seedlings enter the material receiving box, and then the overall survival rate and the yield of Chinese orchid adult seedlings are effectively increased.

The invention discloses a direct seeding and seedling raising composite biological matrix for cremastra appendiculata and a use method of the composite biological matrix. The matrix is prepared from orthopaedic saw dust, a plant material compound, a composite functional fungicide and a mineral additive, the plant material compound is prepared from vinasse, furfural residues, turfy soil or / and medicine residues and waste lentinus edodes fungus bag residues; the effective viable count of the composite functional microbial inoculum in the composite biological matrix is greater than or equal to 5 billion CFU / g, and the composite functional microbial inoculum is formed by mixing bacillus subtilis, brevibacillus laterosporus and trichoderma harzianum according to the mass ratio of (2-4): (2-4): (3-5); the mineral additive is formed by mixing perlite and vermiculite according to the ratio of 1: 1. The composite biological substrate realizes combination of physical and chemical properties, biological prevention and control and nutrition supply through raw material synergy and process optimization, is suitable for the fields of agricultural traditional Chinese medicinal material seedling substrates, culture substrates, bio-organic fertilizer carriers and the like, improves the use effect through optimization of a traditional Chinese medicinal material rhododendron fargesii planting method, and is suitable for large-scale popularization and application in the fields of agricultural traditional Chinese medicinal material seedling substrates, culture substrates, bio-organic fertilizer carriers and the like. And expected social and economic benefits are achieved.

The invention provides a method for regulating and controlling soilless low-temperature vernalization and arrow drawing of cymbidium goeringii, and belongs to the technical field of cymbidium goeringii flowering phase regulation and control. The method comprises the following steps: (1) washing the root system of cymbidium goeringii, and airing to separate out plants with flower buds; (2) loading the plant root with the flower bud into a container containing a culture medium; (3) wrapping with a buffer product; (4) placing the plants in a refrigeration house to complete vernalization; and (5) spraying a plant growth regulator, wrapping the buffer product, and then transferring into an environment with the day temperature of 23-27 DEG C and the night temperature of 13-17 DEG C for flower forcing culture, so that the plant is subjected to arrow drawing, namely the vernalization and arrow drawing process of cymbidium goeringii is completed. According to the method for soilless low-temperature vernalization of the cymbidium goeringii and growth promotion of the arrows, the flowering period of the cymbidium goeringii is manually regulated and controlled, out-of-season or timed flowering of the cymbidium goeringii is achieved, the production cost is reduced, the efficiency is improved, and meanwhile the commodity value and market adaptability of the cymbidium goeringii are improved.

The present disclosure provides a plant composition comprising a plant extract, wherein the plant extract is present in the plant composition in an amount effective to produce a beneficial effect on intestinal health of an animal. The benefits may include one or more effects on the intestinal health of the animal, which may include one or more improvements in the intestinal health and immune health of the animal. The plant extracts can comprise dandelion leaf extracts, dandelion leaves, ground dandelion leaves, dandelion roots, panax japonicus, ferula asafetida, ligustrum lucidum, fennel, Indian mulberries, quispualis indica, capsicum annuum, Indian nelumbo nucifera, red peppers / capsicum, shiitake mushroom, custard apples, hericium erinaceus, caraway, saw palmetto, phoenix-tail cymbidium, mountain lily yucca, carob beans, wild lupin, goat heads, sweet rosa roxburghii tratt, damnacanthus indicus and herba lycopi; , Amaranthus spinosus, and Sambucus williamsii, or any combination thereof. Other aspects are also provided herein.

The invention discloses a method for inducing an intermediate propagule of cymbidium faberi, and belongs to the technical field of rapid propagation of orchid plants. Aiming at the problems that the cymbidium faberi is low in division reproduction rate, carries viruses and the like, the method comprises the following steps: culturing a rare line art cymbidium faberi seedling serving as an explant in a proper culture medium, and directly inducing an intermediate propagule. By utilizing the technology, the intermediate propagules can be obtained in batches in a short time, and a large batch of cymbidium faberi adult seedlings with stable hereditary characters can be rapidly obtained after differentiation culture, so that technical support is provided for large-scale breeding of the rare cymbidium faberi.

The present invention relates to a culture medium and method for promoting rapid seedling formation of Tiangui Cymbidium orchid seeds, and relates to the fields of plant biotechnology and plant cultivation technology. The culture medium includes a protocorm induction culture medium, a cluster bud differentiation culture medium, a cluster bud proliferation culture medium, and a rooting and seedling strengthening culture medium; the culture method includes the following specific steps: (1) sterile sowing and protocorm induction; (2) cluster bud differentiation culture; (3) cluster bud proliferation culture; and (4) cluster bud rooting and seedling strengthening culture. The present invention uses seeds of Tiangui Cymbidium orchid from different plants and flowers for cross-pollination as explants to construct a sterile sowing and culture system, which promotes rapid seed germination and a high germination rate, overcomes the disadvantages of using bulbs or tender shoots as explants, such as waste of materials and low induction rate, while improving production efficiency and reducing production costs.