12 results about "Cymbidium" patented technology

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The present application relates to the fields of molecular biology and plant breeding technology, and particularly relates to a primer pair for distinguishing between one-flowering and continuous-flowering Cymbidium varieties, application and kit thereof. The primer pair comprises FRI-M-F and FRI-M-R. The molecular marker FRI-M-F / FRI-M-R provided by the present application can accurately and efficiently distinguish between one-flowering Cymbidium varieties and continuous-flowering Cymbidium varieties, and can be used for molecular marker assisted screening and identification of the continuous-flowering trait of Cymbidium, thereby significantly improving the breeding efficiency.

The application discloses a Cymbidium cultivation method capable of controlling gradual color change and a novel color Cymbidium, and relates to the technical field of plant genetic engineering. The method comprises the following steps: constructing a fusion expression vector containing a P2A self-cleavage peptide, introducing the vector into Agrobacterium to obtain an engineered Agrobacterium, introducing the engineered Agrobacterium into a Cymbidium receptor material by using an Agrobacterium-mediated transformation method, and culturing, screening positive transformants by observing red pigment accumulation under environmental light, and adjusting transformation conditions to realize the synergistic accumulation or different expression levels of anthocyanins and betalains. The method realizes independent expression of double genes from a single mRNA, realizes device-free screening by combining RUBY visual marking, realizes color gradient control by transformation condition regulation, can realize stable coexistence of anthocyanins and betalains in the same tissue of Cymbidium, creates novel colors such as a purplish red color system or a gradual color, and shortens the transformation period to 5 days.

The application discloses a Cymbidium SSRR primer group and a method for constructing a Cymbidium variety fingerprint by using the primer group. The Cymbidium SSRR primer group comprises 14 pairs of SSR primer sequences. The 14 pairs of SSR primer sequences are used for carrying out multiplex PCR amplification on Cymbidium genomic DNA, the PCR products are detected by using capillary electrophoresis fluorescence, SSR marker data are analyzed, 70 kinds of Cymbidium SSR fingerprint maps are constructed, and Cymbidium varieties can be effectively identified. The method for constructing the Cymbidium variety fingerprint has the advantages of high throughput, high precision and low cost, and has a good application prospect.

The invention relates to the field of cosmetics and biotechnology thereof, in particular to a vesicle composition with a corn germ extract wrapped by black orchid cells as well as a preparation method and application of the vesicle composition. The vesicle composition disclosed by the invention is obtained by feeding a corn germ extract into a fermentation system of black orchid cells. The culture conditions of the fermentation system of the black orchid cells are as follows: the temperature is 22-25 DEG C, the speed is 120-180 rpm, and the culture period is 15-20 days. The corn germ extract can be added in a delay phase, an exponential phase, a later exponential phase and a plateau phase of fermentation culture of black cymbidium cells. The black cymbidium cell vesicle composition obtained by the invention has the advantages of low immunogenicity, enhanced cellular uptake, higher stability and targeting ability, and better affinity with a phospholipid bilayer of a skin cuticle. And after reaching the cuticle of the skin, active matters can be quickly released, so that the effects are achieved. The compound can be used for preparing antioxidant or anti-aging medicines or cosmetics.

This invention provides a method for screening and grading quality indicators for Rhododendron seedlings, thereby determining grading standards and classifying seedling quality. Through systematic testing of various quality indicators of one-year-old Rhododendron seedlings, and by comprehensively applying multivariate statistical analysis, principal component analysis was used to extract the three key indicators with the highest contribution, serving as the main basis for seedling grading. Combined with K-means clustering algorithm, seedlings were scientifically divided into four quality grades based on these core indicators. Verification has shown that these grading indicators can effectively reflect the growth of Rhododendron seedlings after transplanting, providing a scientific basis for quality control of Rhododendron seedlings in production.

The invention provides a method for regulating and controlling soilless low-temperature vernalization and arrow drawing of cymbidium goeringii, and belongs to the technical field of cymbidium goeringii flowering phase regulation and control. The method comprises the following steps: (1) washing the root system of cymbidium goeringii, and airing to separate out plants with flower buds; (2) loading the plant root with the flower bud into a container containing a culture medium; (3) wrapping with a buffer product; (4) placing the plants in a refrigeration house to complete vernalization; and (5) spraying a plant growth regulator, wrapping the buffer product, and then transferring into an environment with the day temperature of 23-27 DEG C and the night temperature of 13-17 DEG C for flower forcing culture, so that the plant is subjected to arrow drawing, namely the vernalization and arrow drawing process of cymbidium goeringii is completed. According to the method for soilless low-temperature vernalization of the cymbidium goeringii and growth promotion of the arrows, the flowering period of the cymbidium goeringii is manually regulated and controlled, out-of-season or timed flowering of the cymbidium goeringii is achieved, the production cost is reduced, the efficiency is improved, and meanwhile the commodity value and market adaptability of the cymbidium goeringii are improved.

1. Name of the designed product: building blocks (Cymbidium). 2. Use of the designed product: for toys. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

This invention provides a *Ceratophyllum demersum* strain and its applications. The *Ceratophyllum demersum* strain has the accession number CCTCC NO: M20252398 at the China Center for Type Culture Collection, with a deposit date of October 30, 2025. The *Ceratophyllum demersum* strain of this invention can synergistically promote the multi-stage development of *Cymbidium* without requiring the replacement of different fungi, simplifying the propagation process; it reduces competition between fungal species and symbiotic instability caused by environmental factors, improving the consistency of germination rate and seedling survival rate. It eliminates the need for multi-strain staged inoculation and culture management, reducing operational steps and costs, making it suitable for greenhouse seedling cultivation and large-scale propagation.

The invention provides a tea processing device and an orchid tea processing system, and belongs to the technical field of orchid tea production. The tea processing device comprises a rolling mechanism and an air supply mechanism. A containing groove is formed in the inner ring wall of a rolling disc of the rolling mechanism, the inner diameter of a matching ring is smaller than that of the rolling disc, and the matching ring is matched in the containing groove. The outer side wall of a rolling barrel of the rolling mechanism is simultaneously attached to the inner ring wall of the matching ring and the inner ring wall of the rolling disc, so that the inner ring wall of the matching ring is flush with the inner ring wall of the rolling disc at the position attached to the rolling barrel, and the inner ring wall of the matching ring is located on the side, close to the central axis of the rolling disc, of the inner ring wall of the rolling disc at other positions. The air supply mechanism communicates with the containing groove. The tea leaf rolling device can further improve the tea leaf rolling sufficiency, prevents the tea leaves from remaining at the edge of the rolling disc, improves the rolling output rate, and reduces the cleaning workload at the same time.