219 results about "Panax japonicus" patented technology

The invention discloses application of panax japonicus transcription factor gene PjWRKY1, namely, application in improving the expression quantity of key enzyme genes in the biological synthesis of panax japonicus saponins, and increasing the content of saponins in panax japonicus callus. The nucleotide sequence of the PjWRKY1 gene is shown as SEQ ID NO: 1, and WRKY transcription factors are coded. Through the adoption of functional genomics and techniques related to metabolic engineering, a panax japonicus PjWRKY1 transcription factor is proved to have the effect of positively regulating the biological synthesis of panax japonicus saponins; when the panax japonicus PjWRKY1 transcription factor gene disclosed by the invention is established on a plant expression vector and transferred into the panax japonicus callus for over expression, the expression quantity of the key enzyme genes in the synthetic route of panax japonicus saponins is increased, and the yield of panax japonicus saponins is increased.

The invention discloses a ginseng identification method and a special kit. The method comprises the steps: a) extracting genomes DNA as templates from a to-be-tested sample and utilizing primers (sequence 1 and sequence 2) to perform PCR (Polymerase Chain Reaction) amplification; b) adding a fluorescent dye SYBR Green I to the amplified reaction system and determining whether the to-be-tested sample contains ginsengs according to the following methods; if green fluorescence is observed in the reaction system, the to-be-tested sample or the candidate product contains ginsengs; if no green fluorescence is observed in the reaction system, the to-be-tested sample or the candidate product does not contain ginsengs. The experimental results show that the rapid and accurate identification of ginsengs, American ginsengs, pseudo-ginsengs, panax japonicus, rhizoma panacis majoris and bipinnatifid ginseng rhizome can be realized by the method provided by the invention; the fluorescent dye method is introduced to detect true or false identification results and technical supports are provided to realize onsite application of identification of medicinal molecules.

The invention discloses panax japonicus polysaccharide.The main chain structure thereof is Beta-D-1 to 3-mannan and/or Alpha-D-3 to 4-mannan and heterosaccharide containing galactan, xylan and glucan, the weight-average molecular weight of the panax japonicus polysaccharide is 5,000 to 2,000,000, the intrinsic viscosity at 25 DEG C is 1 to 100ml/g, and the mole ratio of the three elements of C: H: O is 1: 2: 1. The preparation of the panax japonicus polysaccharide comprises the following steps: after being dried, smashed and skimmed, the panax japonicus is soaked by water under the normal temperature and is stirred for separation, the residue is extracted by high-pressure hot water, further extracted by alkali and reductive salt, and finally extracted by acid, and the panax japonicus polysaccharide which can be dissolved in water and DMSO and not be dissolved in methanol, ethanol and acetone is obtained by separation, neutralization by taking the solution, dehydration, protein removal, decolorization, desalination and drying. The panax japonicus polysaccharide which is prepared by the invention has higher anti-tumor activity and promotion of immune activity, which can be used for preparing drugs or health care products for improving immune function and anti-tumor. The method for preparing the product is simple and easy to operation, and the sources of the needed raw materials are wide.

The invention provides a traditional Chinese medicine immunopotentiator for enhancing an immune function of broiler chickens during growth. The immunopotentiator comprises crude medicines such as aleppo avens, panax japonicus, platycodon grandiflorum, pokeberry roots, fructus psoraleae, hawthorn fruits, Chinese yams, honeysuckle, sargentgloryvine stems, fructus cnidii, artemisia apiacea, bighead atractylodes rhizome, cinnamon, sealwort, wolfberries, radix glehniae, angelica sinensis, isatis roots, mistletoe, polygonum multiflorum, herba fibraureae recisae, thlaspi arvense, gynostemma pentaphylla and liquorice. The traditional Chinese medicine immunopotentiator has functions of increasing weight and promoting growth of the broiler chickens, has a function of promoting the immune function, can enhance the organism immunity, can improve the anti-bacterial and anti-virus capability and can improve the meat quality. The traditional Chinese medicine immunopotentiator plays an important role in adjusting immune organs, immune cells and the specific immune function of the broiler chickens and can effectively reduce drug residues and pathogenic microorganism residues of animal products.

The invention relates to a preparation method of total rhizoma panacis japonica saponins and the application thereof in the medicine for treating myocardial ischemia. Coarse powder of panax japonicus medicinal material is obtained, and coarse sizing ethanol solution is added in to dip in; the solution is extracted after being heated and refluxed, and the extracting solution is merged; the solution is decompressed to recover solvent until no odor of ethanol exists; the solvent is dilluted with water and is let stand, and the solution is centrifugated to obtain supernatant fluid; the supernatant fluid is extracted by using normal butanol containing water in an equivalent way, and the extract liquid is merged; the merged extract liquid is decompressed to recover the solvent until no normal butanol is distilled; thick extract is fully dissolved by using the ethanol solution of 85 percent, and acetone is added in slowly and the stirring is performed at the same time; then the solution is let stand under a normal temperature to lead the precipitate to be fully separated and is centrifugated, the precipitate is collected and is washed by the acetone, and the total rhizoma panacis japonica saponins is obtained after being dried. The invention can simply and effectively extract the total rhizoma panacis japonica saponins from the panax japonicus, the maximum metastasis rate of the total rhizoma panacis japonica saponins can reach 97.81 percent to show that the extraction technology is more stable and reliable, and the extraction rate is higher; the medicine which is obtained by utilizing the total rhizoma panacis japonica saponins as a main constituent has better curative effect for treating the myocardial ischemia.

The present invention provides a composition containing an extract from Wisteria floribunda, Myristica fragrans, Punica granatum, Trapa bispinosa or a derivative thereof, Coix lachiryma-jobi, Elfvingia applanata and Ganoderma lucidum or a derivative thereof (optionally further containing Panax ginseng or Panax japonicus), which has a superior antiviral activity, a superior peripheral blood flow-improving activity and a superior hair growth-stimulating activity, and a production method thereof.

The invention discloses a method for preparing panax japonicus saponin IVa and an application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase. The preparation method mainly comprises the following steps: crushing panax japonicus saponin IVa bulk pharmaceutical chemicals, extracting with water or a hydrophilic organic solvent, filtering and concentrating extract; passing the concentrated solution through a macroporous adsorption resin column chromatography column so as to enrich total saponin, eluting with water and an alcohol-water mixedliquid in turn, collecting the alcohol-water eluent, performing reduced pressure concentration at a low temperature, and drying to obtain the total saponin; and carrying out chromatograph separation on the total saponin, eluting with a solvent, recovering the solvent at reduced pressure, and drying so as to obtain crude panax japonicus saponin IVa; and repeatedly recrystallizing the crude productwith an organic solvent so as to obtain pure panax japonicus saponin IVa. According to verification, the panax japonicus saponin IVa has bioactivity of protecting liver and lowering transaminase, andcan be mixed with one or more of pharmaceutically acceptable carriers/auxiliary materials so as to be prepared into solid and liquid oral preparations with effects of protecting liver and lowering transaminase.

The invention relates to a micro-ecological synbiotic Chinese herbal medicine feed addictive, comprising a micro-ecological synbiotic feed addictive and a Chinese herbal medicine additive, wherein the micro-ecological synbiotic feed addictive comprises micro-ecological probiotics and oligosaccharide, the micro-ecological probiotics is a lactic acid bacillus preparation, and the oligosaccharide isone or more of fructo-oligo saccharide, xylo oligosaccharide, isomaltose oligosaccharide and soybean oligosaccharide, the weight of the micro-ecological synbiotic feed addictive accounts for 25-75 % of that of the total weight of the micro-ecological synbiotic Chinese herbal medicine feed addictive, and the Chinese herbal medicine additive comprises the following ingredients by weight parts: malt, pericarpium citri reticulatae viride, dried orange peel, rhizoma atractylodis, medicated leaven, coke hawthorn, coke gardenia, Chinese medical stone, folium artemisiae argyi, eucommia, dichroae radix, and panax japonicus. Separate application of the additive can raise animal culture production efficiency, reduce feeding cost, enhance the immunity and anti-stress capability of animals, and reduceincidence of diseases. Combined application of the additive and the Chinese herbal medicine additive has significant synergism.

The invention relates to a Panax japonicus var.major seedling method and culture method; the method can realize mutual promotion and cooperation of various special technical means, thus obviously improving seed germination rate and seedling emergency percentage, and greatly reducing seedling time; in the culture method, specific fertilizers are used so as to obviously improve transplanted survival rate and final yield; the Panax japonicus var.major seedling method and culture method can provide excellent application prospect and promotion potential in the Panax japonicus var.major planting field.

The invention relates to the technical field of stem cells and especially relates to an umbilical cord mesenchymal stem cell injection liquid, a preparation method and an application thereof. The injection liquid comprises umbilical cord mesenchymal stem cells, human albumin, low molecular heparin calcium, vitamin C, panax japonicus majoris saponin and a solvent. The injection liquid is prepared from the umbilical cord mesenchymal stem cells, so that the materials are convenient to obtain and the method does not violate ethical issue. The injection liquid can effectively prolong preservative time of the cells and the preserved stem cells have high activity, so that the injection liquid is convenient to use. After refusion, the injection can promote differentiation and maturation of the mesenchymal stem cell to liver cells, thus reducing incidence rate of GVHD.

The invention relates to the field of plant cell culture and provides a method for acquiring secondary metabolites including panax japonicus saponins and the like with large-scale culture and a bioconversion technology for panax japonicus cells. The method comprises steps as follows: (1), auxins, cytokinins and a coagulator are added to an RW culture medium; (2), explant surface of panax japonicas is sterilized and callus is formed through induction; (3), the callus of the panax japonicas is subjected to subculture, and loose cell clusters are obtained; (4), a culture solution is prepared; (5), the loose cell clusters are transferred into the culture solution for culturing; (6), high-yield cell strains are cultured and screened; (7), the high-yield cell strains are subjected to multiplication culture; (8), a synthetic medium is prepared; (9), cells are continuously cultured in the synthetic medium, and bioconversion is completed; (10), purification is performed after extraction with a solvent. With the adoption of the method, the secondary metabolites of the panax japonicas can be produced, the production cost is low, the culture period is short, the method is not limited by natural environment, and annual production can be realized.

The invention discloses a Chinese herbal medicine extracting solution capable of preventing hair loss and holding hair. The Chinese herbal medicine extracting solution is prepared from the following ingredients in parts by weight: 30-50 parts of angelica sinensis, 45-70 parts of the root of three-nerved spicebush, 15-37 parts of crocus sativus, 15-50 parts of panax japonicus, 30-66 parts of garden nasturtium, 45-70 parts of polygonum multiflorum, and 50-90 parts of ligusticum wallichii. The Chinese herbal medicine extracting solution capable of preventing hair loss and holding hair can induce the hair, promote the hair growth, promote the hair follicle cell proliferation, prevent hair loss and hold the hair. The invention further discloses a preparation method of the Chinese herbal medicine extracting solution capable of preventing hair loss and holding hair, and the method is less in step, simple in procedure, safe in operation and low in cost. The invention further discloses a hair-growing cream and a preparation method thereof. The hair-growing cream is capable of effectively downward permeating from the healthy hair follicle of the hair to activate nearby necrotic hair follicle cells, thereby achieving the effect of growing new hair.

The invention discloses application of panax japonicus transcription factor gene PjbHLH1, namely, application in improving the expression quantity of key enzyme genes in the biological synthesis of panax japonicus saponins, and increasing the content of saponins in panax japonicus callus. The nucleotide sequence of the PjbHLH1 gene is shown as SEQ ID NO: 1, and bHLH transcription factors are coded. Through the adoption of functional genomics and techniques related to metabolic engineering, a panax japonicus PjbHLH1 transcription factor is proved to have the effect of positively regulating the biological synthesis of panax japonicus saponins; when the panax japonicus PjbHLH1 transcription factor gene disclosed by the invention is established on a plant expression vector and transferred into the panax japonicus callus for over expression, the expression quantity of the key enzyme genes in the synthetic route of panax japonicus saponins is increased, and the yield of panax japonicus saponins is increased.

The invention provides an organic fertilizer applicable to culturing of ginseng in farmlands and a preparation method of the organic fertilizer. According to the preparation method of the organic fertilizer, organic matters can be adequately decomposed, the transformation of the organic matters can be promoted, and available nutrients of the organic matters can be increased; secondly, the total amount of beneficial microorganisms of leavenings is greatly increased, and the number of harmful bacteria is greatly decreased; besides, the zymolysis of half-mature composts and livestock manures can be promoted, the fertilizer efficiency is improved, and the content of available nutrients of general composts prepared from same raw materials is increased by 20%-30%; finally, by adding nutrient substances such as humic acid according to the nutrient proportion of a basic organic fertilizer, the indexes of the prepared organic fertilizer can meet requirements of the culturing of ginseng in farmlands to soil, and the organic fertilizer is especially applicable to the culturing of ginseng in the farmlands.

The invention relates to a biological soil conditioner for cultivation of ginseng. The conditioner is characterized in that the conditioner comprises the following raw materials in parts by weight: 50-70 parts of corn straw, 20-30 parts of bean cakes, 5-10 parts of wheat bran, 4-8 parts of a 5% allicin micronemulsion, and 1-2 parts of a composite microbial inoculant fermentation stock solution. The biological soil conditioner can be used for improving farmland soil for cultivation of ginseng and long-cultivated land soil for cultivation of ginseng, effectively controlling soil borne diseases of ginseng, and at the same time improving yield of ginseng; and the conditioner is adapted to be applied to woodland cultivation and farmland cultivation of ginseng.

The invention provides a panax japonicus extract, comprising an IVa compound as an active component or a pharmaceutically acceptable salt. The invention also provides a monomeric compound extracted from panax japonicas root and stem. The monomeric compound comprises a panax japonicas extract according to the claim 1, and pharmaceutically acceptable auxiliary material and carrier. The panax japonicas extract is applied to preparation of medicines for treating intestinal mucosal injuries; the intestinal mucosal injuries are intestinal mucosal injuries caused by non-steroidal anti-inflammatory drugs. The result shows that the intestinal mucosal injuries of a medication administration team of the panax japonicas IVa and permeability transition are obviously reduced; and the panax japonicus IVa has therapeutic effect on intestinal mucosal injuries caused by the non-steroidal anti-inflammatory drugs.

The invention discloses deep caring and moisturizing aqua-essence which is prepared from the following raw materials in parts by weight: 8-16 parts of a seaweed extract, 8-16 parts of a ginseng extract, 4-8 parts of rose essential oil, 1-3 parts of tea tree leaf oil, 3-6 parts of diazolidinyl urea, 15-30 parts of deionized water, 5-12 parts of betaine, 7-14 parts of carbomer, 1-3 parts of sorbitol, 2-6 parts of soybean oil, 7-14 parts of vitamin E, 3-6 parts of propylene glycol, 7-9 parts of sodium hyaluronate and 5-10 parts of hydrogenated castor oil. The invention provides deep caring and moisturizing aqua-essence which has the effects of repairing deep necrotic skin, moisturizing, hydrating skin and reducing wrinkles.

The invention relates to application of panax japonicus IV a in prevention and treatment of cardiovascular disease and complication of the cardiovascular disease. Experiments show that the panax japonicus IV a has obvious protection effect on ischemia/reperfusion C57 mouse myocardium, the optimum dose is determined, and a medicine for preventing and treating the cardiovascular disease and related complication of the cardiovascular disease can be further prepared.