21 results about "Panax japonicus" patented technology

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The invention discloses a method for preparing rare saponin by utilizing panax japonicus endogenous enzyme, and relates to the technical field of extraction, the panax japonicus plant material or crude extract thereof is directly taken as a starting point, the enzyme system is simple and convenient to obtain (fresh medicinal material low-temperature homogenate extraction or direct use of freeze-dried powder), the reaction system is simple, and the operation is easy. According to the preparation process provided by the invention, the catalytic action of endogenous enzyme is fully utilized, so that the introduction of exogenous substances (acid-base, protein and the like) in the extract is completely avoided; the method disclosed by the invention is high in catalytic efficiency, can quickly realize complete conversion of saponin (within 10min) under specific conditions, and has extremely high industrial application.

The invention discloses application of panax japonicus polysaccharide in preparation of drugs for resisting porcine reproductive and respiratory syndrome virus. According to the application disclosed by the invention, the regulation and control effect of the panax japonicus polysaccharide on the replication of the porcine reproductive and respiratory syndrome virus (PRRSV) is studied, and the fact that the panax japonicus polysaccharide can effectively inhibit the replication of the PRRSV on a Marc-145 cell is found for the first time. On the basis, the invention provides a new application of the panax japonicus polysaccharide in preparation of the medicine for resisting the porcine reproductive and respiratory syndrome virus, and preferably, the concentration of the panax japonicus polysaccharide is 12.5 mu g / mL-100 mu g / mL. The research on the antiviral activity of the panax japonicus polysaccharide is enriched, and meanwhile, a new technical means is provided for research and clinical application of anti-PRRSV drugs.

The invention provides application of a pharmaceutical composition in preparation of anti-influenza virus drugs, and belongs to the technical field of biological medicines. The invention provides a pharmaceutical composition of carabrone, panax japonicus saponin IVa and plantamajoside. Multi-target intervention is realized aiming at key stages (such as adsorption, replication and release) of an influenza virus life cycle. Experiments show that the composition can significantly inhibit replication and proliferation of influenza viruses. The natural component composition disclosed by the invention has the advantage of higher safety, provides a safer, effective and economic treatment choice for clinic, and has remarkable conversion value and market potential.

This invention provides a traditional Chinese medicine compound for treating chronic pharyngitis, belonging to the field of biomedical technology. The invention comprises a self-formulated traditional Chinese medicine compound consisting of *Pearl Umbrella*, *Trichosanthes kirilowii*, *Sapindus mukorossi*, *Lycoperdon perlatum*, *Panax japonicus*, *Gnaphalium affine*, *Aster tataricus*, and prepared licorice root for the treatment of chronic pharyngitis. Addressing the complex pathogenesis of chronic pharyngitis, characterized by a mixture of deficiency and excess, phlegm and blood stasis, and yin deficiency, the ingredients in the compound are rationally combined and work synergistically to achieve the effects of promoting blood circulation without depleting yin, clearing heat without harming yang, resolving phlegm without hindering the spleen, nourishing yin without being cloying, combining tonification and purgation, using both warming and cooling methods, and treating both the root cause and the symptoms. This compound is suitable for the treatment and management of chronic pharyngitis.

The invention provides a seedling raising method for aseptic seedlings of panax japonicus. The seedling raising method comprises the following steps: step 1, obtaining disinfected tender leaves; step 2, obtaining sterile panax japonicus leaves; step 3, transferring the sterile panax japonicus leaves obtained in the step 2 to a callus induction culture medium for culture to obtain panax japonicus leaf calluses; step 4, transferring the panax japonicus leaf calluses to a subculture medium for culture to obtain panax japonicus leaf subculture callus particles; step 5, transferring the callus to an adventitious bud culture medium for induction to obtain panax japonicus adventitious buds; step 6, transferring the adventitious buds of the panax japonicus leaves to a bud generation subculture medium for culture to obtain panax japonicus cluster buds; step 7, culturing the cluster buds on a rooting culture medium for 35 days for rooting to obtain aseptic seedlings of the panax japonicus; according to the method, callus induction, adventitious bud and rooting induction formula screening is carried out based on the panax japonicus leaf explant, and a reference basis is provided for efficient establishment of a panax japonicus aseptic seedling system.

The present invention belongs to the field of Chinese medicine compositions, and in particular to a Chinese medicine composition for preventing and / or treating chronic kidney disease vascular calcification, and its preparation method and use. The Chinese medicine composition is a preparation prepared from the following raw materials in parts by weight: 5-15 parts of Radix Dichroae, 5-20 parts of Eucommia, 5-20 parts of Cistanche, 5-15 parts of Cuscuta, 5-20 parts of Rehmannia, 5-20 parts of Epimedium, 5-15 parts of Rhizoma Drynariae, 5-15 parts of Panax Japonicus, 5-20 parts of Millettia Spatholobi, and 5-20 parts of Semen Persicae. The Chinese medicine composition of the present invention can improve calcium-phosphorus metabolism disorder, protect the cardiovascular system, improve renal function, enhance cardiac function, inhibit vascular calcification, and prevent the transdifferentiation of vascular smooth muscle cells, with "tonifying the kidney, activating blood circulation and unblocking collaterals" as the treatment principle, by regulating the kidney-bone-vascular axis, inhibiting oxidative stress and inflammation, and intervening in calcium-phosphorus metabolism, thereby effectively treating CKD vascular calcification.

The invention belongs to the technical field of food processing, and particularly relates to a panax japonicus and dendrobium protocorm co-extract as well as a preparation method and application thereof. The preparation method of the co-extract comprises the following steps: (1) extraction: mixing panax japonicus and dendrobium protocorm, adding a natural deep eutectic solvent, controlling the solid-to-liquid ratio to be 1: (30-50) g / mL, and extracting under an ultrasonic condition to obtain a crude extract; (2) anti-solvent precipitation and purification: dropwise adding the crude extract obtained in the step (1) into excessive ethanol, standing for precipitation, and carrying out centrifugal separation to obtain a precipitate; and (3) drying treatment: drying the precipitate obtained in the step (2) at 40 DEG C until the weight is constant, so as to obtain the panax japonicus and dendrobium protocorm co-extract. The co-extract can remarkably promote proliferation and acid production of various probiotics, effectively regulate intestinal flora balance, show excellent prebiotic activity and have the potential of being developed into functional medicines or health-care foods.

The present invention relates to the field of traditional Chinese medicine analysis technology, and particularly relates to a detection method and application of differential components of traditional Chinese medicine belonging to the genus Panax. The present invention uses the technology of online comprehensive two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for metabolite characterization, and combines non-target metabolomics technology. In the detection of component differences of traditional Chinese medicine belonging to the genus Panax, it can perform component difference analysis on large-scale and multi-batch traditional Chinese medicine belonging to the genus Panax, which helps to deeply understand the component differences of traditional Chinese medicine belonging to the genus Panax.

This utility model discloses a storage and preservation device for bamboo-joint ginseng, including a sealed box. A light-shielding layer is fixedly installed on the inner wall of the sealed box, and the box is divided into multiple inner cavities by at least one dividing layer. A drawer unit is slidably installed in each inner cavity, and the bottom of the drawer unit is provided with a wavy, hollowed-out elastic silicone mesh tray. The light-shielding layer is connected to the lower part of the dividing layer via a disassembly and assembly mechanism. This utility model offers upgraded physical protection: the hollowed-out structure of the wavy, elastic silicone mesh tray disperses the stress on the bamboo-joint ginseng nodes, resulting in a reduced fracture rate when the ginseng is subjected to a 500g load. The light-shielding layer is efficiently disassembled and assembled: the Y-shaped insertion groove and the inclined guide design of the limiting block shorten the replacement time of the light-shielding layer, and the elastic component automatically locks to prevent loosening.

The invention discloses a skin care composition, a preparation method and skin care cream, and relates to the technical field of skin care products. Raw materials of the skin care composition comprise decarboxylated carnosine, a plant fermentation extract, a traditional Chinese medicine composition and the like, the traditional Chinese medicine composition is an extract of centella asiatica and phyllanthus emblica, and plant raw materials of the plant fermentation extract comprise bulbs of lilac and roots, stems and leaves of panax plants. By compounding the decarboxylated carnosine, the plant fermentation extract and the specific traditional Chinese medicine composition, the effects of inhibiting melanogenesis, regulating the skin state and promoting repair are synergistically exerted, and meanwhile, by adopting a step-by-step fermentation and oil-water phase split preparation process, the skin care cream developed by the invention has the effects of keeping skin elasticity and stimulating skin immunity, and the skin care cream has a good application prospect. The skin state can be obviously improved.

The invention provides application of a traditional Chinese medicine composition in preparation of a medicine for delaying senescence. The traditional Chinese medicine composition is prepared from traditional Chinese medicinal materials including ginseng, rhizoma polygonati, rhizoma atractylodis, radix sophorae flavescentis, radix ophiopogonis, rehmannia, polygonum multiflorum, dogwood, poria cocos, eupatorium, coptis chinensis, rhizoma anemarrhenae, herba epimedii and the like. Experiments prove that the pharmaceutical composition can significantly reduce the expression of P16, P21, TP53BP1 and gamma-H2AX in pancreas, reduce the content of TNF-alpha, IL-6 and IL-1beta in pancreas, reduce the number of pro-inflammatory immune cells CD80 +, CD4 + and CD8 + in pancreas, increase the number of anti-inflammatory immune cells CD206 + in pancreas, and delay pancreas aging.

The invention belongs to the field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for preventing and / or treating chronic kidney disease vascular calcification and a preparation method and application thereof. The traditional Chinese medicine composition is a preparation prepared from the following raw material medicines in parts by weight: 5-15 parts of yam rhizome, 5-20 parts of eucommia ulmoides, 5-20 parts of cistanche, 5-15 parts of semen cuscutae, 5-20 parts of rehmannia, 5-20 parts of epimedium, 5-15 parts of rhizoma drynariae, 5-15 parts of panax japonicus, 5-20 parts of caulis spatholobi and 5-20 parts of peach kernel. The traditional Chinese medicine composition can improve calcium and phosphorus metabolism disorder, protect a cardiovascular system, improve kidney functions, enhance heart functions, inhibit vascular calcification and prevent transdifferentiation of vascular smooth muscle cells, takes the therapeutic principle of tonifying kidney, promoting blood circulation and dredging collaterals, intervenes calcium and phosphorus metabolism by adjusting kidney-bone-vascular axes, inhibiting oxidative stress and inflammation and treating the liver and kidney diseases, and has a good treatment effect. Therefore, CKD vascular calcification can be effectively treated.

The invention discloses a children growth promoting preparation, which belongs to the technical field of traditional Chinese medicine preparations and comprises fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger, angelica sinensis, fructus amomi, radix inulae inula, pericarpium citri reticulatae, serissa serissoide, panax japonicus, rhizoma cibotii, radix scrophulariae and cattle bone powder. The preparation integrates the traditional Chinese medicine theory of tonifying spleen and kidney and regulating qi and blood, wherein the fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger and the like can tonify spleen and promote appetite, significantly improve the digestive function and improve the absorption rate of nutrient substances; the Tibet inula root and the fructus amomi regulate middle-jiao qi movement, relieve abdominal distension and qi stagnation and further optimize the absorption state; the panax japonicus, the rhizoma cibotii and the cattle bone powder directly act on a skeletal system to tonify kidney and strengthen bone, promote cartilage cell proliferation and bone longitudinal growth, and effectively improve height and growth speed. The formula gives consideration to intervention on growth disorder problems such as precocious puberty and the like, and through two-way regulation of nourishing yin and purging fire and tonifying kidney and replenishing essence, advanced bone age is delayed, and precious time is won for height increase.

A vomit-stopping bead patch belongs to the technical field of medical supplies and comprises vomit-stopping beads and an acupoint patch. The vomit-stopping beads are thuja sutchuenensis medicine beads and are prepared by soaking thuja sutchuenensis scattered beads in a new liquid preparation formed by mixing traditional Chinese medicine extract, ginger juice and essential balm. The traditional Chinese medicine extract is prepared by decocting 100g of all-grass of aleppo akebia, 100g of cynanchum auriculatum, 100g of dutchmanspipe root, 100g of panax japonicus, 100g of akebia fruit, 100g of fructus evodiae, 100g of persimmon stem and 100g of ginger processed pinellia with water and concentrating; the thuja sutchuenensis loose beads are made of thuja sutchuenensis wood and are made into beads with the particle size of 0.5-0.7 mm. The thuja sutchuenensis loose beads have the beneficial effects that the vomit-stopping bead plaster is applied to and presses corresponding acupuncture points of a patient, vomiting and nausea of the chemotherapy patient can be effectively relieved, and the thuja sutchuenensis loose beads have good popularization and application values.

Provided is a microvesicle composition for reducing or inhibiting inflammatory expression, a method for preparing same, and use thereof. The composition comprises a mesenchymal stem cell microvesicle and a plant cell microvesicle. The plant is selected from any one of lonicera, schisandra, zingiber, astragalus, ophiopogon, panax, and momordica, or a combination thereof.

The application discloses application of a substance for detecting SNP polymorphism in identification or auxiliary identification of Panax quinquefolium, and belongs to the field of nucleic acid determination or testing methods in biochemistry. The technical problem to be solved by the application is how to identify or assist in identifying Panax quinquefolium. In the application of the substance for detecting SNP polymorphism in identification or auxiliary identification of Panax quinquefolium, the SNP is SNP2, the SNP2 is a SNP of a chloroplast genome of Panax, and is the 164th nucleotide in SEQ ID No. 5 in the sequence table, and the nucleotide is C or T. The application can accurately distinguish Panax quinquefolium and other species of Panax, and can be used for identification and auxiliary identification of Panax quinquefolium.

The present disclosure provides a plant composition comprising a plant extract, wherein the plant extract is present in the plant composition in an amount effective to produce a beneficial effect on intestinal health of an animal. The benefits may include one or more effects on the intestinal health of the animal, which may include one or more improvements in the intestinal health and immune health of the animal. The plant extracts can comprise dandelion leaf extracts, dandelion leaves, ground dandelion leaves, dandelion roots, panax japonicus, ferula asafetida, ligustrum lucidum, fennel, Indian mulberries, quispualis indica, capsicum annuum, Indian nelumbo nucifera, red peppers / capsicum, shiitake mushroom, custard apples, hericium erinaceus, caraway, saw palmetto, phoenix-tail cymbidium, mountain lily yucca, carob beans, wild lupin, goat heads, sweet rosa roxburghii tratt, damnacanthus indicus and herba lycopi; , Amaranthus spinosus, and Sambucus williamsii, or any combination thereof. Other aspects are also provided herein.

The invention discloses panax japonicus formula granules and a preparation method thereof, and the preparation method comprises the following steps: (1) repeatedly crushing panax japonicus into superfine powder, and then breaking walls by using a wall breaking machine; (2) adding water or an ethanol solution into a wall breaking machine, carrying out wall breaking extraction, filtering, and concentrating filtrate into extract; and (3) adding auxiliary materials into the concentrated extract to prepare granules. Compared with previous extraction or heating or reflux extraction, wall-breaking extraction is adopted, the extraction efficiency of active ingredients such as saponin and polysaccharide is high, in-vitro and in-vivo blood glucose reducing experimental models show that the prepared granules have a good blood glucose reducing effect and small side effects, a simple, convenient and rapid formula selection is provided for clinical doctors to make a prescription, and the preparation process is simple and convenient. The novel traditional Chinese medicine granule decoction piece convenient to take and carry is provided for patients, the medication compliance is enhanced, and the good social and economic effects are achieved.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a preparation method and application of a Gastrodia elata Yin-boosting oral preparation. The oral preparation comprises the following raw materials in parts by weight: Gastrodia elata 20-25 parts, Rehmannia glutinosa 15-20 parts, Cornus officinalis 14-17 parts, Fructus Lycii 17-21 parts, Rubus corchorifolius 15-20 parts, Anoectochilus roxburghii 10-15 parts, Ophiopogon japonicus 10-20 parts, Polygonatum odoratum 10-14 parts, Panax ginseng 5-10 parts, Atractylodes macrocephala 7-12 parts, Poria cocos 5-10 parts, Ziziphus jujuba var. spinosa 10-15 parts, Schisandra chinensis 5-10 parts, Angelica dahurica 5-8 parts, Angelica sinensis 1-5 parts, and Glycyrrhiza uralensis 3-8 parts. Animal experiments prove that the oral preparation can prolong the exhaustive swimming time of mice, reduce the content of blood lactic acid and serum urea of mice after swimming, increase the storage of liver glycogen, and has a significant anti-fatigue effect.

This invention discloses the application of *Gynostemma pentaphyllum* polysaccharide in the preparation of drugs against porcine reproductive and respiratory syndrome virus (PRRSV). This invention explores the regulatory effect of *Gynostemma pentaphyllum* polysaccharide on PRRSV replication, and for the first time discovers that *Gynostemma pentaphyllum* polysaccharide can effectively inhibit PRRSV replication in Marc-145 cells. Based on this, this invention proposes a new application of *Gynostemma pentaphyllum* polysaccharide in the preparation of drugs against PRRSV, preferably with a concentration of 12.5 μg / mL to 100 μg / mL. This invention enriches the research on the antiviral activity of *Gynostemma pentaphyllum* polysaccharide and also provides a new technical means for the research and clinical application of anti-PRRSV drugs.