106 results about "Microvesicle" patented technology

The invention discloses a foaming agent and circulative micro-foam drilling fluid as well as well-drilling technological method; wherein, the foaming agent comprises 30% neopelex ABS, and 70% lauryl sodium sulfate SDS; the circulative micro-foam drilling fluid is produced through adding 0.1% sodium hydroxide, 0.2 to 0.3% xanthan gum XC, 0.2 to 0.3% medium viscosity flocculent cellulose CMC and 0.1 to 0.3% foaming agent; during well drilling construction, the circulative micro-foam drilling fluid is first to be prepared, and then is used to perform the wet drilling. With the foaming agent added to the circulative micro-foam drilling fluid, the formed micro-foam slurry has the advantages of good leakage proof and plugging effect, great lubrication performance, good rheology, obvious improved drilling quality and drilling efficiency.

The invention relates to a formulation comprising a dry material (e.g. lyophilised or spray dried) comprising at least one film forming surfactant and a gas or a gas mixture usable in diagnostic imaging, to a process for the preparation thereof and to a suspension obtainable by reconstituting said formulation with an aqueous carrier for use in contrast imaging. The invention also relates to a container comprising the dry material in contact with a gas. The gas associated with the dry material is at a pressure lower than the atmospheric pressure.

The present invention relates to direct protein delivery with engineered micro vesicles.

The invention relates to a polyamine micromolecular compound, comprising the following structures described in the specification, wherein M<x+> is 0, Zn<2+>, Ga<3+>, Gd<3+>, Ca<2+> or other divalent metal ions and trivalent metal ions; S is a reporter group (including a nuclide labeling prothetic group, a paramagnet, a fluorescein or a microvesicle), such as the nuclide labeling prothetic group: -<11>CH3, -CH2CH2<18>F, -CH2CH2(OCH2CH2)2NHCOC6H4<18>F-p or -CH2CH2(OCH2CH2)2NH-CH2CH2<18>F; R is -H, -OCH3, -OCH2CH3 or -Cl; and R1, R2, R3 and R4 are hydrogen, carboxyl, alkane, alkylene or heteroalkyl. The invention further relates to application of the compound in preparing cell death or apoptosis developers of target phosphatidyl serine (PS) and/or apoptotic cell early free Zn<2+>. The compound provided by the invention is a specific multiamine micromolecular developer for target phosphatidyl serine (PS) and/or apoptotic cell early free Zn<2+>, which can be used for monitoring curative effects of PS-related anti-tumor chemotherapy, radiotherapy, biological therapy and the like; the compound can be used for early differential diagnosis of neurodegenerative diseases (senile dementia and Parkinson's disease), cerebral apoplexy, AIDS (Acquired immune deficiency syndrome), thrombus, atheromatous plaque and myocardial infarction; and the compound can also be used for differential diagnosis and curative effect monitoring of other diseases related to PS expression in the cell death or apoptosis process, such as inflammation development and anti-inflammation therapeutic development.

A system based on envelope microvesicle for perfusion imaging and ultrasonic controlled release is composed of ultrasonic imaging probe, fully digitalized ultrasonography B, RF data output port, high-speed data acquisition card, PC as main controller, any waveform generator, 3D moving unit, moving controller and single-cell transducer. On the basis of fully digitalized ultrasonography B, a blood perfusion imaging algorithm is used for making a decision to obtained original RF data and performing real-time imaging. Under the guide of perfusion image, the release of envelope microvesicles is controlled.

The invention discloses a photoacoustic microscope. The photoacoustic microscope comprises a microscope system, a signal collection control system and a driving system. The invention further discloses a method for monitoring breaking of microvesicles in biological tissue with the use of the photoacoustic microscope. The photoacoustic imaging is combined with features of high contrast ratio of optical imaging and high resolution ratio of acoustic imaging. Due to low scattering properties of acoustic signal transmission in tissue, deeper penetration degree is obtained compared with a conventional optical imaging method. There is no need to slicing tissue and the method is non-invasive so that long-term monitoring is achieved. Photoacoustic microscope imaging equipment helps achieve imaging of multiple anatomical positions.

The invention discloses a dual tangential flow filtration system for exosome extraction and a preparation method and application of an exosome. The method comprises the following steps: putting a sample solution containing exosomes into a first storage tank, pumping the sample solution into a first filtering device, circularly concentrating and filtering, and collecting a filtrate into a second storage tank; when a volume of the liquid in the first storage tank is concentrated to the minimum operation volume, pumping the liquid in the second storage tank into a second filtering device, and carrying out circulating concentration and filtration; and collecting the liquid in the second storage tank to obtain the extracted and purified exosome solution. The exosome can be used for preparing exosome medical beauty products, exosome liquid dressings for promoting wound healing, exosome spray for relieving asthma and acute respiratory distress, and exosome related biological products. According to the invention, the exosome is extracted and purified through two tangential flow filtering membranes with different pore diameters, so that cell microvesicles and cell debris with larger particles in a sample can be removed, and the exosome can be extracted and concentrated to the greatest extent.

A method for detecting biomarkers of prostate cancer or other medical condition of the prostate based on the use of microvesicles obtained from urine samples, and the nucleic acids present in the microvesicles. The method disclosed herein are advantageous in that they may be used to support diagnosis, prognosis, monitoring, or therapy selection in lieu of or in conjunction with traditional biopsy-based diagnostics and do not require a digital rectal examination or prostate massage prior to urine sample collection.

The invention provides inflatable nano microvesicle high-ash coal slime flotation equipment. The inflatable nano microvesicle high-ash coal slime flotation equipment comprises a main flotation columnand two secondary flotation columns, the main flotation column comprises an agent box, an ore pulp preprocessor, a high-speed disperser and a delivery pump which are mounted outside the main flotationcolumn, and an ore pulp multi-spot distributor mounted on the main flotation column, a foam collecting device, an atomizing spraying device, a bubble generator surrounding the outer wall of the lowerportion of the main flotation column, a conveying air pipe, an air storage tank and an air compressor are further included. The main flotation column and the secondary flotation columns are adopted for sequentially selecting concentrate, middlings and tailings are removed, and clean coal products with the ash content of 11% or below are finally obtained.

The invention relates to novel biomarker signature, diagnostic methods, kits and compositions for early diagnosis of ovarian cancer, based on microvesicles prepared from body fluid sample, specifically, uterine lavage fluid (UtLF) sample.

A freeze-dried powder composition comprising a phospholipid and a polyethylene glycol, said polyethylene glycol having a percentage of folded polymeric chains higher than a predetermined limit. The composition is suitable for preparing gasfilled microvesicles.

A method of delivering exosomes and other micro vesicles to a biological target includes the steps of 1) providing blood, (2) separating plasma from the provided blood, and (3) separating the solution with the exosomes therefrom, (4) encapsulating the exosomes, and (5) delivering the exosomes. The step of encapsulation may be accomplished by water bead process, alginate bead process, spray drying bead formation, or plating. The biological target may be a human or animal heart, bone/joint, wound or skin. A method of encapsulating an exosome, a method of using an encapsulated exosome, compositions including encapsulated exosomes, and an encapsulated exosome per se are also disclosed.

The invention provides a phase change nano droplet regulation and control method based on a spatially heterogeneous focused eddy sound filed. The phase change nano droplet regulation and control method based on the spatially heterogeneous focused eddy sound filed comprises the steps that by adjusting relative power and a phase position among spherical focused ultrasonic transducer array elements,the heterogeneous focused eddy sound filed with symmetric distribution of sound pressure and a sound pressure maximum value existing on an annular antinode is generated, the heterogeneous focused eddysound filed is used for aggregating scattered phase change nano droplets to a micron-sized bulk droplet aggregate, so that a phase change threshold value of the phase change nano droplets is obviously reduced, a single microsecond short pulse is used for acting on the aggregated phase change nano droplets, then droplet phase change is induced, and the nano size distribution of the droplets in theaggregate ensures the vibration activity of phase change microvesicle. According to the phase change nano droplet regulation and control method based on the spatially heterogeneous focused eddy soundfiled, sound regulation and control of the phase change nano droplets under a living body condition has higher flexibility and safety.

The invention discloses a culture medium for producing alginate lyase by fermentation of a microvesicle bacterial genus. The culture medium comprises a strain culture medium and a fermentation culture medium. The invention further discloses a fermentation method for producing alginate lyase by the fermentation of microvesicle bacterial genus. According to the fermentation method, on the basis of shake flask fermentation, the screened microvesicle bacterial genus is subjected to fermentation culture with a fermentation tank, a research on fermentation condition optimization is conducted, and a lyase producing rule of the microvesicle bacterial genus in the fermentation tank is researched, so that the yield of alginate lyase is increased effectively; and alginate lyase produced by the fermentation of the microvesicle bacterial genus ALW1 is subjected to pilot scale amplification fermentation, so that important technical parameter guide is provided for future mass production.

The invention discloses applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury. Applications of mesenchymal stem cell derived and gene modified miR-34a-MSC-MV, which are specifically designed microvesicles, in preparation of drugs for treating renal injury are included. The level of miR-34a is detected by inducing the overexpression of the miR-34a in MSCs through lentiviral vectors. Through the discovery from pathological observation, the damage level of renal tissue can be obviously improved, renal interstitial fibrosis, lymphocyte infiltration and swelling and necrosis degrees of renal tubules can be obviously alleviated, and renal histopathological scores can be significantly reduced. Through in vivo and in vitro experiments, repair effects of MV and miR-34a-MV are verified; the effects of gene modified stem cells and paracrine products thereof are cleared; and an action mechanism is preliminarily discussed.

The invention discloses a gene-carrying developing microvesicle used for gene targeted therapy of an ischemic heart disease, and a preparation method of the gene-carrying developing microvesicle. Thegene-carrying developing microvesicle comprises a positive ion developing microvesicle and therapeutic gene plasmids dispersed on the shell surface of the developing microvesicle, wherein the developing microvesicle is provided with a positive-electricity lipid bimolecular layer shell and biological inert gases coated in the shell; and the therapeutic gene plasmids include plasmids containing a tissue inhibitor of metaslloproteinase-3 (TIMP-3) gene and a silent information regulator 3(SIRT3) gene. A gene microvesicle preparation carries out intensive cavitation and releasing on a target gene under an ultrasound function, quickly carries out gene transfection on a target organ, is high in transfection efficiency and a targeting ability, is safe and noninvasive and can be repeatedly operated. In addition, the gene-carrying developing microvesicle can simultaneously carry out developing under ultrasound, detects and diagnoses a perfusion situation of ischemic myocardium and can carry outaccurate assessment while the therapy is carried out, and therefore, the therapy is more accurate.

The invention discloses a urine exosome extraction reagent tube and a manufacturing method, the urine exosome extraction reagent tube comprises a cap, an outer tube and an inner tube, the top end surface of the outer tube is sleeved with the cap through threads, the inner tube is sleeved with the outer tube, and the inner wall of the inner tube is provided with a nanofiber membrane. The whole reaction can be completed in a short time, and long-time waiting is not needed; by utilizing a positive and negative charge attraction principle, not only is the pollution of a separation reagent avoided, but also non-charged lipoprotein with the particle size of 50-200nm in the urine can be removed; the pore size of the positive charge porous nanofiber membrane is about 50-200nm, so that the high-purity exosome with the particle size of 50-200nm can be obtained, and the pollution of microvesicles with other particle sizes can be prevented; and the porous structure on the positive charge porous nanofiber membrane greatly increases the contact area between the membrane and a sample, and increases the extraction efficiency of the urine exosome.