Applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury

Pending Publication Date: 2021-08-20
西安市第一医院
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Problems solved by technology

However, it is not yet clear whether the function of microvesicles to transport gene information plays an important role in stem cell remodeling and tissue regeneration, and whether it helps stem cells repair damaged tissues through paracrine action, which is very meaningful and worthy of further study Questions discussed

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  • Applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury
  • Applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury
  • Applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] 1. In vitro experiment:

[0032] 1. Culture of human proximal renal tubular epithelial cells (HK2): HK2 was purchased from ATCC, USA. The M199 culture solution containing 5% newborn fetal bovine serum and penicillin / streptomycin (100 μg / L) was used for cultivation in a 37° C. incubator (5% CO 2 ), and the medium was changed every 2 days. When the cell density reaches 80%-90%, it is digested with 0.25% trypsin (containing 1 mmol / L EDTA) and subcultured ...

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Abstract

The invention discloses applications of gene modified mesenchymal stem cell derived microvesicles in preparation of drugs for treating renal injury. Applications of mesenchymal stem cell derived and gene modified miR-34a-MSC-MV, which are specifically designed microvesicles, in preparation of drugs for treating renal injury are included. The level of miR-34a is detected by inducing the overexpression of the miR-34a in MSCs through lentiviral vectors. Through the discovery from pathological observation, the damage level of renal tissue can be obviously improved, renal interstitial fibrosis, lymphocyte infiltration and swelling and necrosis degrees of renal tubules can be obviously alleviated, and renal histopathological scores can be significantly reduced. Through in vivo and in vitro experiments, repair effects of MV and miR-34a-MV are verified; the effects of gene modified stem cells and paracrine products thereof are cleared; and an action mechanism is preliminarily discussed.

Description

technical field [0001] The invention relates to the technical field of stem cell microvesicles, in particular to the application of gene-modified microvesicles derived from bone marrow mesenchymal stem cells in the preparation of drugs for treating kidney damage. Background technique [0002] The incidence rate of Chronic Kidney Disease (CKD) in my country is as high as 11.8%, and it has become another major cause of threatening human health after cardiovascular and cerebrovascular diseases, tumors and diabetes. Tubulointerstitial inflammation and fibrosis are the main pathological basis leading to the progression of CKD, which is characterized by the progressive loss of normal nephrons and the proliferation of a large number of fibroblasts and excessive secretion of extracellular matrix. This process is similar to that after tissue injury. Scar repair rather than normal tissue repair. The current clinical treatment measures can only delay the speed of this scar repair proc...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28C12N5/10C12N15/113A61P13/12
CPCA61K35/28A61P13/12C12N5/0663C12N15/113C12N2310/141
Inventor 何娟王妍东瑞娟王汉民
Owner 西安市第一医院
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