The invention relates to application of a ZC3H12b
gene or
protein and a method for establishing a hepatic
disease animal model. According to the invention, a
gene editing technology is utilized, the zc3h12b
gene of oryzias latipes is knocked out in a targeted mode, the oryzias latipes with Zc3h12b
protein product deficiency are established, all the oryzias latipes show different degrees of hepatobiliary duct
hyperplasia and fusion,
hepatocyte steatosis,
fibrosis and other hepatic lesions, the obvious
fatty liver appears along with the month-old growth, local
cyst necrosis occurs,
lymphocyte infiltration is obvious in the hepatic sinusoid, the number of macrophages is increased abnormally, positive cells of
human tumor markers CK19, SMA and GPC3 are detected, and it is suggested that ZC3H12b can serve as a therapeutic target and a biomarker of intrahepatic biliary cystadenoma, intrahepatic biliary cystadenocarcinoma or the related
fatty liver or
liver cancer. The zc3h12b-deficient oryzias latipes can be used as an
animal model for researching the intrahepatic biliary cystadenoma, the intrahepatic biliary cystadenocarcinoma or the
lesion process of the intrahepatic biliary cystadenoma and the intrahepatic biliary cystadenocarcinoma.