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Application of Cbl-b protein in preparation of product with protective effect on lung injury induced by tobacco smoke

A protective effect, cbl-b technology, applied in the field of genetic engineering, can solve the problems such as the lack of Cbl-b and COPD research, to achieve the effect of protecting lung damage, reducing infiltration, and slowing down the decline of lung function

Pending Publication Date: 2021-04-30
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no research on Cbl-b and COPD in the world

Method used

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  • Application of Cbl-b protein in preparation of product with protective effect on lung injury induced by tobacco smoke
  • Application of Cbl-b protein in preparation of product with protective effect on lung injury induced by tobacco smoke
  • Application of Cbl-b protein in preparation of product with protective effect on lung injury induced by tobacco smoke

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Experimental program
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Effect test

Embodiment 1

[0033] (1) COPD animal model preparation

[0034] Commissioned Guangzhou Saiye Biotechnology Co., Ltd. to use CRISPR / Cas9 technology to establish Cbl-b gene knockout (Cbl-b - / - ) mice. Method: According to the gene information and experimental requirements of Cbl-b, a guide RNA vector targeting the gene coding region was designed and constructed, and the constructed vector and Cas9 were transcribed into mRNA in vitro, and the obtained transcribed RNA was injected into fertilized eggs. The fertilized eggs successfully microinjected were returned to the surrogate mother mice, and the mice born by the mother mice were identified by sequencing to obtain F0 generation positive mice. F0 generation positive mice (heterozygous mice) and wild type (Cbl-b + / +) mice were mated, and the born mice were identified by PCR to obtain 50% positive F1 generation heterozygous mice. Select the F1 generation heterozygous mice from the same F0 generation positive mouse, and get the male and femal...

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Abstract

The invention discloses an application of Cbl-b protein in preparation of a product with a protective effect on lung injury induced by tobacco smoke. The product with a protective effect on lung injury induced by tobacco smoke is a medicine for promoting Cbl-b protein expression. The invention also discloses a medicine with the protective effect on lung injury induced by tobacco smoke, and the medicine is beneficial to protecting lung injury induced by tobacco smoke, slowing down the decline of lung function, reducing lung tissue macrophage and lymphocyte infiltration, relieving lung tissue inflammatory response, reducing the degree of emphysema; and slowing the epithelial-mesenchymal transition process of lung tissues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to the application of Cbl-b protein in the preparation of products with protective effect on lung damage induced by tobacco smoke. Background technique [0002] Chronic obstructive pulmonary disease (COPD; COPD) is a respiratory disease characterized by chronic airway inflammation and emphysema, accompanied by progressive decline in lung function, which can seriously affect the quality of life of patients. High rates and mortality. In 2007, the prevalence rate of COPD in people over 40 years old in my country was as high as 8.2%, but it continued to increase to 13.7% in 2015. The number of COPD patients in my country has exceeded 99.9 million, which shows the rapid growth rate of the number of patients. The pathogenesis of COPD is closely related to smoking, air pollution, pathogen infection and other risk factors and genetic susceptibility factors. -Related...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K38/53A61K48/00A61K49/00A61P11/00
CPCA61K48/005A61K38/53A61K45/00A61P11/00A61K49/0008C12Y603/02019
Inventor 卢文菊陈玲珠王彦王健徐静仪李媛媛张子丽袁良
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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