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Corneal midterm preservation solution and preparation method thereof

A technology for preserving liquid and cornea, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of affecting the visual function of patients, corneal opacity, decreased transparency, etc., and optimize the decellularization conditions and morphology of animal matrix. Good, simple ingredients

Inactive Publication Date: 2009-08-12
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Irreversible damage to the corneal endothelium will lead to corneal opacity and decreased transparency. However, for patients who need corneal transplantation, this will undoubtedly affect the recovery of visual function and the improvement of visual acuity

Method used

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  • Corneal midterm preservation solution and preparation method thereof
  • Corneal midterm preservation solution and preparation method thereof
  • Corneal midterm preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Based on 100ml of preservation solution, 84.5ml of MEM culture medium is used as the basic culture medium, containing 10ml of fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, 25mmol of HEPES, and 2.5g of chondroitin sulfate and low molecular weight dextran 1g, the pH value is 7.2-7.4, and the osmotic pressure is 350-380mOsm / L.

[0032] Preparation method 1: Add 2.5g of chondroitin sulfate and 1g of low molecular weight dextran to 10ml of fetal bovine serum, 1ml of 100mM non-essential amino acids, 1ml of 100mM sodium pyruvate, 2.5ml of 1M HEPES, 1ml of 10% tobramycin and 84.5ml of MEM Mix well in the culture solution, adjust the pH to 7.2-7.4, and the osmotic pressure to 350-380mOsm / L, filter and sterilize through a 0.2μm membrane, store in 20ml aliquots at 4°C to obtain this preservation solution. Add sodium bicarbonate to adjust the pH to 7.3-7.4. Each specimen is 20ml, and the required growth factors can be app...

Embodiment 2

[0035] Based on 100ml of preservation solution, 84.5ml of M199 culture medium is used as the basic culture medium, which contains 10ml of premium fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, 25mmol of HEPES, etc., and can also contain Chondroitin sulfate 2.5g, low molecular weight dextran 1g, pH value 7.2-7.4, osmotic pressure 350-380mOsm / L.

[0036] Preparation method 1: Add 2.5g of chondroitin sulfate and 1g of low molecular weight dextran to 10ml of special grade fetal bovine serum, 1ml of 100mM non-essential amino acid, 1ml of 100mM sodium pyruvate, 2.5ml of 1M HEPES, 1ml of 10% tobramycin and 84.5 Mix well with M199 culture medium, adjust pH to 7.2-7.4, osmotic pressure to 350-380mOsm / L, filter and sterilize through 0.2μm membrane, aliquot 20ml and store at 4°C to obtain this preservation solution. Add sodium bicarbonate to adjust the pH to 7.3-7.4. Each specimen is 20ml, and the required growth factors can be ...

Embodiment 3

[0039] Based on 100ml of preservation solution, 42.25ml of MEM culture solution and 42.25ml of M199 culture solution are used as the basic culture solution, containing 10ml of premium fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, and 25mmol of HEPES etc., can also contain chondroitin sulfate 2.5g, low molecular weight dextran 1g, pH value is 7.2-7.4, osmotic pressure is 350-380mOsm / L.

[0040] Preparation method 1: Add 2.5g of chondroitin sulfate and 1g of low molecular weight dextran to 10ml of special grade fetal bovine serum, 1ml of 100mM non-essential amino acid, 1ml of 100mM sodium pyruvate, 2.5ml of 1M HEPES, 1ml of 10% tobramycin, 42.25 Mix ml MEM culture solution and 42.25ml M199 culture solution evenly, adjust the pH value to 7.2-7.4, osmotic pressure to 350-380mOsm / L, filter and sterilize through 0.2μm membrane, store in 4°C after 20ml aliquots liquid. Add sodium bicarbonate to adjust the pH to 7.3-7.4. Ea...

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Abstract

The present invention provides a liquid for preserving cornea for medium period and a preparing method thereof, and relates to a liquid for cornea material. The invention provides the liquid for preserving cornea for medium period and the preparing method thereof, wherein the liquid has the advantages of longer storing time, higher quality, no pollution and relatively lower cost compared with theprior liquid for preserving cornea for medium period. The liquid for preserving cornea for medium period contains culture solution, thickening agent, bovine serum, amino acid, antibiotic, acid-alkalimodifying agent and cell nutrition components. According to the formula of liquid for preserving cornea for medium period, the thickening agent, the bovine serum, the amino acid, the antibiotic and cell nutrition components are added into the culture solution for mixing to uniform. The pH value is adjusted to 7.2-7.4 with the acid-alkali modifying agent. The osmotic pressure is adjusted to 350-380mOsm / L with an osmotic pressure buffering agent. The liquid for preserving cornea for medium period is obtained after membrane filtering for sterilizing.

Description

technical field [0001] The present invention relates to a corneal material preservation solution, in particular to a method for treating corneal trauma, tumors, chemical burns (alkali burns), vascularized severe dry eye, limbal stem cell deficiency, immune diseases such as Stevens-Johnson syndrome ( SJS), ocular pemphigoid, herpes simplex virus leukoplakia, corneal transplantation rejection and other blinding eye diseases corneal mid-term preservation solution and preparation method thereof. Background technique [0002] According to the report of the World Health Organization, corneal disease is the second major cause of vision loss. Every year, 1.5 to 2 million new corneal blindness are caused by corneal ulcers and eye trauma. The main cause of childhood blindness is corneal leukoplakia caused by measles . The only effective treatment for corneal blindness is keratoplasty. However, due to the influence of religious beliefs and customs, very few people donate corneas afte...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 刘祖国邹文进
Owner XIAMEN UNIV
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