131 results about "Tobramycin" patented technology

A method for treatment, prevention and containment of acute and chronic tuberculosis using a preservative-free concentrated tobramycin aerosol formulation delivering tobramycin to the lung endobronchial space including alveoli in an aerosol having mass medium average diameter predominantly between 1 to 5 mu . The method comprises administration of tobramycin in concentration one to ten thousand times higher than the minimal inhibitory concentration of Mycobacterium tuberculosis. A method for containment of and decreasing infectivity periods of tuberculosis patients to shorter periods of time.

Provided are electrokinetically-altered fluids (gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellular membrane potential and cellular membrane conductivity, and therapeutic compositions and methods for using same in treating cystic fibrosis or a symptom thereof. The electrokinetically-altered fluid compositions and methods include electrokinetically-altered fluids optionally in combination with other therapeutic agents (e.g., antibiotics, albuterol, budesonide, etc.). Particular embodiments comprise use and/or synergy with tobramycin for treating bacterial infection, and use and/or synergy with a bronchiodilator. In certain aspects, the methods comprise regulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins (like, membrane receptors, including but not limited to G protein coupled receptors, and intercellular junctions).

Provided are electrokinetically-altered fluids (gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellular membrane potential and cellular membrane conductivity, and therapeutic compositions and methods for using same in treating cystic fibrosis or a symptom thereof. The electrokinetically-altered fluid compositions and methods include electrokinetically-altered fluids optionally in combination with other therapeutic agents (e.g., antibiotics, albuterol, budesonide, etc.). Particular embodiments comprise use and/or synergy with tobramycin for treating bacterial infection, and use and/or synergy with a bronchiodilator. In certain aspects, the methods comprise regulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins (like, membrane receptors, including but not limited to G protein coupled receptors, and intercellular junctions).

A patient suffering from an endobronchial infection is treated by administering to the patient for inhalation a dose of less than about 4.0 ml of a nebulized aerosol formulation comprising from about 60 to about 200 mg/ml of an aminoglycoside antibiotic, such as tobramycin, in a physiologically acceptable carrier in a time period of less than about 10 minutes. Unit dose devices for storage and delivery of the aminoglycoside antibiotic formulations are also provided.

A wound spacer device comprising multiple beads connected by non-absorbable suture material is disclosed. The device can be applied, for example, by a first responder to an injured individual, or can be applied by a trauma treatment facility, such as a Level 2 medical unit. In typical embodiments the device allows for site-specific controlled elution of an antimicrobial agent, such as Tobramycin, including defined elution over a period of time, such as 48 or 72 hours.

This invention relates to formulations for topical use comprising antibiotics in combination with anti-inflammatory steroids for treating ophthalmic infections and attendant inflammation. More specifically, this invention relates to pharmaceutical ophthalmic formulations comprising a pH stabilizing amount of tobramycin and the soft steroid loteprednol etabonate.

The present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. A suitable antibiotic is selected from the group consisting of doxycycline, gentamicin, tobramicin, ciprofloxacin, clindamycin, clarithromycin, azithromycin and metronidazole. Preferred antibiotics are doxycycline and ciprofloxacin. More preferably, the antibiotic is doxycycline. In its second aspect, the present invention is directed to a method for treating periodontal disease in a mammalian patient comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. Typically, the mammalian patient is a human.

Disclosed is a cornea middle term preserving fluid which comprises RPMI 1640 culture liquid, chondroitin sulfate, hyaluronic acid, HEPES cushioning liquid, dexamethasone, low-molecular-weight seaweed polysaccharides, Tobramycin or vancomycin, the preserving fluid has stabilized cell activity during storage period, and higher endothelial cell metabolizing function can be maintained than the US's Opthiol.

Methods for the treatment of bacterial infections in the respiratory system of a subject, such as the lungs of a subject, using fusidic acid alone or in combination with a second bacterial agent such as tobramycin, amikacin, fosfomycin or levofloxacin are described.

The invention describes compositions and kits comprising at least one nitric oxide enhancing group antimicrobial compound, or pharmaceutically acceptable salts thereof, and novel compositions comprising at least one nitric oxide enhancing antimicrobial compound, and, optionally, at least one nitric oxide enhancing compound and/or at least one therapeutic agent. The invention also provides methods for (a) treating bacterial infections; (b) treating viral infections; (c) treating fungal infections; and (d) treating lesions. The antimicrobial compounds of the invention are preferably tobramycin, aztreonam, ciprofloxacin and doripenam. The nitric oxide enhancing antimicrobial compounds are substituted with at least one heterocyclic nitric oxide donor group and/or at least one nitroxide group. The nitric oxide enhancing groups are nitroxides and/or heterocyclic nitric oxide donors. The heterocyclic nitric oxide donors are furoxans, sydnonimines, oxatriazole-5-ones and/or oxatriazole-5-imines. In one embodiment the methods of the invention are for the treatment of bacterial infections associated with pulmonary diseases such as cystic fibrosis and for treating Bacillus anthracis infections.

The invention relates to a kind of compound collagen protein gutta, which is the mixture of collagen protein and antibiotics. For every 100ml compound collagen gutta, there is 10-500mg collagen protein which is extracted from the tendon, 100-600mg antibiotics which include one or two kinds of the following antibiotics: ofloxacin, alficetin, tenebrimycin, while the rest is the iso-osmia, which is 5% amylaceum solution. The product has three main functions: resist bacteria and inflammation; help to keep the eyeball moist, promote the agglutination and relieve the asthenopia; be used in the treatment of the damage to the cornea, keratohelcosis, keratitis punctata, slight chemical injury to cornea as well as the thermal damage to the cornea; prevent the ophthalmia and relieve the asthenopia; prevent the cohesive property of the collagen from being diluted so as to extend the effective time of the gutta; resist the bacteria as well as promote the agglutination.

The invention discloses an eye use compound drug combination containing lotepredenol etabonate and tobramycin. The combination contains less than 0.1 percent of tetra chloro metlbond. The preparation method of the drug combination is simple and is suitable for industrial production.

Vectors expressing kanA-kanB-kanK and other kanamycin production-related genes, Streptomyces<i/> species recombinant bacteria transformed with the vectors, a method of producing kanamycin antibiotics by the bacteria, and a new kanamycin compound produced by the bacterium are provided. With the use of the recombinant bacteria of the present invention, the direct fermentative biosynthesis of amikacin and tobramycin as semi-synthetic kanamycins is possible, and the yield of kanamycin B as a precursor of the semi-synthetic kanamycin is improved.

The invention relates to the field of drugs, in particular to a detection method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid. A method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through an ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling techniquecomprises the following steps of: 1) preparing reference samples; 2) preparing internal standard solution; 3) preparing sample solution; 4) preparing mobile-phase solution; 5) setting chromatographicconditions; 6) optimizing mass spectrometric conditions; 7) determining the samples; 8) preparing gradient-concentration reference sample solution; 9) preparing a standard curve; and 10) analyzing and computing data. The method has the characteristics that: 1) the method is advanced; 2) the method is rapid and high-efficiency the loss is low; and 3) the clinical application prospect and the effect are good.

The present invention provides the use of an aerosol formulation comprising fosfomycin and tobramycin in the treatment of patients with chronic obstructive pulmonary disease (COPD) who are experiencing or are at risk of experiencing acute exacerbations of COPD. Formulations for such use and methods of treating humans with COPD are also provided.

The invention provides a method for preparing decellularized cornea. Due to the selection of components and proportions, colloid osmotic pressure of a decellularized cornea preparation liquid can be maintained by using a prepared protection liquid, and compared with a conventional decellularized cornea preparation liquid, the decellularized cornea preparation liquid is capable of remarkably alleviating the situation that the cornea transparency is degraded as an extracellular matrix such as collagen is excessively swelled and lost in the later cell treatment process; in addition, due to the addition of antibiotics such as tobramycin, bacterium propagation of susceptible cornea can be inhibited, and the contamination rate in the treatment process can be reduced. By adopting the method provided by the invention, cells can be crushed, and the situation that the trenchancy is degraded as cornea matrix collagen is damaged by long-term high pressure can be avoided. Secondly, as a detergent and nuclease act up simultaneously, cell debris and nucleic acid components can be relatively rapidly and effectively removed, and the situation that the trenchancy of the cornea is degraded as protein components such as cornea collagen are damaged in long-term treatment of the detergent and other components can be avoided.

The invention discloses a colorimetric method for detecting tobramycin based on double strand displacement and a three-dimensional DNA structure, and belongs to the field of food safety, medical analysis and environmental pollution detection. The method comprises the following steps: firstly, double strands T1/T2 are designed; when tobramycin exists, Bsm DNA polymerase synthesizes double strands which are completely complementary through a strong strand displacement reaction, and Nt.BstNBI incision endonuclease cuts recognition sites on the double strands; the three-way DNA structure capture reporter probes, and regenerates and replaces a large number of S1 strands containing G-quadruplex forming sequences. Thereafter, the G-quadruplex/heme catalyzes ABTS<2->/H2O2 chromogenic reaction, andthe tobramycin content can be determined by using the linear relationship between light absorption value and tobramycin concentration. According to the invention, an aptamer captures tobramycin to trigger double strand displacement reaction which is mediated by the Nt.BstNBI incision endonuclease and the Bsm DNA polymerase so as to generate a large number of reporter probes. Meanwhile, the reporter probes trigger lambda exonuclease-assisted loop amplification, so that multiple amplifications of colorimetric signals are realized, the detection range is widened, and the detection sensitivity isimproved.

The invention provides a cornea metaphase preservation solution, and preparing and using methods thereof. The cornea metaphase preservation solution is a cell culture minimum essential medium (MDM) with chondroitin sulfate, low molecular dextran, L-glutamine, dexamethasone, tobramycin, 2-hydroxyethyl and Y-27632 added. The preservation solution not only can keep activity and normal morphology of cornea endothelial cells, but also can enhance viability of corneal limbus epithelial cells and improve clone ability of corneal limbus stem cells. And especially, a medium to long term preservation effect is obvious, phenomena of deformation, conjugation and the like of endothelial cells of a control group cornea do not appear in long term preservation, endothelial morphology of the control group cornea is consistent with endothelial morphology of a cornea preserved for 4 days, and the endothelial cells of the control group cornea are still regular and few in cell conjugation phenomena. The preservation solution can effectively prevent cell apoptosis phenomena during the process of preserving isolated cornea materials, increases activity and clone forming ability of the corneal limbus stem cells, and enables the cornea to maintain a transparent feature in a long preservation time.

The invention relates to a tobramycin inhalation composition and a preparation method and application thereof. The composition is prepared from tobramycin 4%-8% (w/v), sodium chloride 0.1%-0.9% (w/v), weak-reducibility organic weak acid serving as a stabilizer 0.1%-1.0% (w/v), strong acid for regulating the pH value of the composition and the balance water, wherein the pH value of the composition is 5.6-7.5. The tobramycin inhalation composition prepared by adopting the technical scheme has the pH value closer to the physiological pH of a human body and has significantly improved stability at room temperature.

The invention discloses a cornea metaphase preservation liquid and a preparation method thereof. The cornea metaphase preservation liquid is an MEM cell culture medium, which comprises the following components: chondroitin sulfate (20-26 g/L), low molecular dextran (8-12 g/L), L-glutamine (0.37-0.38 mg/L), fetal calf serum (2-5.5%), tobramycin (0.08-0.12 g/L), Hepes (9-10 g/L), and rapamycin (0.9-1.1 nM/L). The provided cornea metaphase preservation liquid can protect corneal endothelial cells, can avoid the phenomenon of necrosis and fusion of endothelial cells due to long time preservation, and prolongs the cornea preservation time more effectively.