16 results about "Tobramycin" patented technology

The present application relates to a kind of reduction response type polyamide-amine dendrimer nanogel and its preparation method, the nanogel with gold nanoparticles, tobramycin, third generation polyamide-amine dendrimer as component, preparation method includes: the preparation of G3-PEG-SAT, the preparation of G3NGs, the preparation of Au@G3NGs and the preparation of Au / Toy@G3NGs.The multifunctional nanogel Au / Toy@G3NGs constructed by the present application provides efficient diagnosis and treatment integrated platform for tumor treatment.

The invention discloses a method for efficiently detecting various microbial limits of tobramycin, belongs to the technical field of microbial detection, and initiates an enzyme-chemical synergistic neutralization system constructed by aminoglycoside-3-acetyltransferase, aminoglycoside-3 '-phosphotransferase, magnesium chloride hexahydrate and a sodium chloride-peptone buffer solution. According to the system, the bacteriostatic activity of tobramycin can be thoroughly eliminated through a direct enzymatic inactivation way, and the limitation that neutralization is not thorough in a traditional method is broken through. Based on the system, the efficient detection method is successfully developed by combining process optimization of adding bacteria and adding a passivating enzyme and a chemical neutralizer into a culture medium. The method has four core advantages of high efficiency, specificity, environmental protection and synergy, can thoroughly eliminate antibacterial interference of tobramycin, and remarkably improves the accuracy and reliability of a microbial limit test result.

The invention discloses programmed release hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method of the programmed water-release hydrogel comprises the following steps: carrying out Schiff base reaction on aldehyde pullulan and aminated hyaluronic acid in water to obtain a first solution; mixing the first solution, acrylamide, a cross-linking agent and tobramycin to obtain a second solution; mixing the quercetin solution with a conductive polymer, and stirring to generate an electrostatic adsorption reaction to obtain a third solution; and mixing the third solution and the second solution, and carrying out free radical polymerization reaction under the action of an initiator to obtain the programmed release hydrogel. The programmed water-release hydrogel prepared by the invention has good biocompatibility, adhesion, self-healing property, conductivity, antibacterial property, anti-inflammatory property and capability of promoting wound healing, and especially has a remarkable effect on treating or improving infectious wounds.

The application discloses an assembled synergistic poly-polypeptide-based biological hybrid material and a preparation method and application thereof, and the preparation steps comprise the following steps: (1) preparation of hybrid bacteria; (2) preparation of side-chain tobramycinized poly-polypeptide; and (3) preparation of poly-polypeptide-based biological hybrid bacterial clusters. The application realizes synergistic antibacterial ability, and promotes the ability of effectively controlling inflammation and wound healing. The unique poly-polypeptide-based biological hybrid structure utilizes a multiplexing and aggregation synergistic design idea, realizes the dual purposes of resisting bacterial infection and regulating host life functions, and significantly improves the comprehensive effect of antibacterial and anti-inflammatory treatment.

The invention successfully constructs a high-sensitivity colorimetric detection method for tobramycin based on flower-like copper sulfide nano enzyme on the basis of peroxidase-like activity of CuS nano enzyme. The flower-like copper sulphide (CuS) nano-enzyme is successfully prepared through a coprecipitation method, the flower-like copper sulphide (CuS) nano-enzyme can catalyze a 3, 3 ', 5, 5'-tetramethyl benzidine-hydrogen peroxide system chromogenic reaction under the acidic condition, and a brand-new colorimetric system for detecting tobramycin is constructed by utilizing the inhibition characteristic of tobramycin on the system. Experimental results show that under the optimal conditions (pH = 4, reaction time 30 min, H220.8 mM, TMB 3.5 mM, and enzyme concentration 15 [mu] g / mL), the detection linear range of the method to the tobramycin is 1 ng / mL-1 mg / mL, the lowest detection concentration is 1 ng / mL, and the sensing system has good specificity to other antibiotics and can effectively distinguish the tobramycin from other antibiotics. The invention not only provides a rapid, sensitive and low-cost new method for detecting tobramycin in water, but also opens up a new thought for antibiotic detection in the fields of environmental monitoring and food safety.

The application discloses a kind of responsive antibacterial hydrogel coating modified implants and preparation method.The present application provides a kind of implant antibacterial hydrogel coating modification method, using hydrogel coating solution is modified implant by immersion, spin coating or spraying method, forms antibacterial coating on the surface of implant;The hydrogel coating solution is composed of tannic acid, 3-formyl phenyl boronic acid, tobramycin and deionized water according to weight ratio (30-60) :(40-70) :(20-45) :1000, pH value is 8.0-10.0.The modification method of the present application is simple in operation, raw material is easy to obtain, process flow is less, and the hydrogel coating does not need initiator and external stimulation, can be in situ gel on the surface of implant at normal temperature and pressure, also has pH, active oxygen stimulation responsiveness and excellent antibacterial performance, and the hydrogel coating of the present application has extensive substrate adhesion, and can be used on the surface of metal, ceramic or composite material etc..

The invention belongs to the technical field of industrial microbial fermentation process optimization, and discloses a bulk drug production strain culture method based on morphology and physiological parameters. According to the method, through dual verification of physiological trend and form peak, the misjudgment risk of single parameter judgment is avoided, meanwhile, the trend is captured as soon as possible through change rate analysis, and transplanting can be conducted at the golden moment when the thallus activity reaches the peak but does not decline. By quantifying morphology and setting a clear threshold value, human subjective factors are eliminated, the process reproducibility is extremely high, the principle of the method is suitable for production of raw material medicines fermented by various filamentous bacteria, such as acarbose, moxidectin and tobramycin, and finally accurate seed transfer is realized, so that the fermentation period is effectively shortened, the yield of the raw material medicines is improved, and the method is suitable for industrial production. And remarkable economic benefits are brought.

The application discloses a kind of nitric oxide donor type raw ginger exosome fusion liposome nanodelivery system and its preparation method and application, the nanodelivery system uses super flow control chip technology to nitric oxide precursor, two stearyl phosphatidylcholine, cholesterol, tobramycin, self-assembly, and then prepared by extrusion method and raw ginger exosome membrane fusion.The drug delivery system has a significant breakthrough in mucus barrier and antibacterial efficacy, and can be applied to inhalation therapy for pseudomonas aeruginosa lung infection.The negative surface of the drug delivery system and the electrostatic repulsion of mucin in mucus, mPEG-PNTC generates NO due to GSH responsiveness, which has a gas motor effect, enhancing the effect of breaking through the airway mucus barrier, while the NO generated by mPEG-PNTC, through nitrosylation and oxidative stress, destroys bacterial biofilm, interferes with bacterial iron metabolism, and synergistically kills bacteria and destroys biofilm.The present application has the advantages of improving drug delivery efficiency and high-efficiency bactericidal function, and has broad clinical application prospects and development value in the field of bacterial lung infection treatment.

The invention discloses a multi-drug resistant pseudomonas aeruginosa PAS57 strain and an application of the multi-drug resistant pseudomonas aeruginosa PAS57 strain. The preservation number of the pseudomonas aeruginosa PAS57 is GDMCC No: 67235. The pseudomonas aeruginosa PAS57 can be used for preparing a bacterial strain. According to the application disclosed by the invention, a KirbyBauer (KB) drug sensitivity experiment shows that the PAS57 is resistant to the levofloxacin, the ciprofloxacin, the meropenem, the imipenem and the tobramycin. The strain disclosed by the invention can be used for screening a novel antibacterial drug which is effective to the multi-drug-resistant pseudomonas aeruginosa, and a new way is provided for development of drugs for treating diseases caused by the pseudomonas aeruginosa.

The invention belongs to the field of fluorescence sensors, relates to detection of tobramycin, and discloses a double-state probe switch type fluorescence sensor as well as a detection method and application thereof. According to the technology, quantitative analysis can be realized without enzyme catalysis or nucleic acid labeling through dynamic balance regulation and control of a G-quadruplex and thioflavin fluorescent system and CHA. The core mechanism is based on base pairing of Apt and cDNA: in the absence of TOB, Apt-cDNA forms a stable double-helix structure, so that HP2-5 rich in G bases spontaneously forms a G-quadruplex, and strong fluorescence is generated after ThT is embedded; according to the sensor, the CHA effect and G-quadruplex dynamic coordinated regulation and control are realized for the first time, complex steps such as enzyme catalysis and fluorescence labeling are not needed in an experiment, the sensor has low detection limit and high accuracy, can be accurately applied to actual sample detection, and a practical solution is provided for detection of TOB residues in animal-derived food.

The invention relates to the field of bacteriostasis, in particular to a bacteriostatic composition containing pyrolin and antibiotics and application of the bacteriostatic composition. The antibacterial composition contains pyrolin and an antibiotic; wherein the antibiotic is selected from at least one kind of materials from tetracycline (Tetracycline), ciprofloxacin (Ciprofloxacin) and tobramycin (Tobramycin). The antibacterial composition disclosed by the invention not only can obviously inhibit various pathogenic bacteria, but also can obviously reduce the use of antibiotics, thereby being beneficial to reducing the possibility of occurrence of bacterial drug resistance.

The application provides a preparation method of g-C3N4 modified polyether sulfone filter membrane, comprising the following steps: S1, preparing g-C3N4 material: taking urea as a precursor, adopting a thermal condensation method to heat, and keeping warm for a period of time, and after cooling, pre-grinding and screening are carried out to obtain g-C3N4 powder; S2, modifying g-C3N4: the g-C3N4 powder prepared in S1 is configured into g-C3N4 water dispersion liquid, then ultrasonic treatment is carried out for a period of time, natural sedimentation is carried out, and the supernatant is taken; polyphenol small molecules and alkali liquor are added into the supernatant to react, then tobramycin is added to react, and after centrifugation, dispersion and freeze-drying, the modified g-C3N4 is obtained; S3, modifying polyether sulfone filter membrane: polyether sulfone, the modified g-C3N4 and an organic solvent are uniformly mixed, heated and stirred for a period of time until completely dissolved, then stirring, degassing are continued until no bubbles are observed, and the casting solution is obtained; the casting solution is formed into a film under certain conditions, and the modified polyether sulfone filter membrane is obtained; the preparation method can improve the hydrophilic property and the anti-pollution property of the filter membrane, and more importantly, the antibacterial property of the film is strengthened.

The application relates to the technical field of intelligent targeted magnetic response micro robots, and discloses a polyphenol-modified magnetic liquid metal-based micro intelligent antibacterial robot and a preparation method thereof.The preparation method comprises the following steps: dissolving polyphenol and an aminoglycoside antibiotic in water, performing a Schiff base reaction to obtain a precursor solution; adding magnetic liquid metal particles into the precursor solution, centrifugally collecting the solid phase after reaction to obtain a precursor; and soaking the precursor in a high agglutinin affinity sugar solution, stirring and reacting, and then centrifugally collecting the solid phase to obtain the polyphenol-modified magnetic liquid metal-based micro intelligent antibacterial robot.The application utilizes the multiple hydrogen bonds of natural polyphenol and the coordination effect of the magnetic liquid metal surface to form a stable interface network, thereby providing a stable support for sugar ligand fixation; meanwhile, tobramycin participates in polyphenol network construction in the form of a Schiff base interaction, realizes the pH-responsive release of the antibiotic, combines and stabilizes mannose, and finally obtains an intelligent targeted antibacterial micro robot.

The invention relates to the field of bacteriostasis, in particular to a composition containing tea polyphenol and antibiotics and application of the composition in bacteriostasis. The composition comprises tea polyphenol and antibiotics, wherein the tea polyphenols are selected from at least one of epicatechin gallate and epigallocatechin, and the tea polyphenols are selected from at least one of epicatechin gallate and epigallocatechin; the antibiotic is selected from at least one of ciprofloxacin and tobramycin. According to the invention, a combined antibacterial scheme of the tea polyphenol and the antibiotics is researched, so that the growth of gram-positive bacteria such as staphylococcus aureus and staphylococcus epidermidis can be obviously inhibited, and the use of the antibiotics can be obviously reduced, thereby being beneficial to reducing the occurrence of bacterial drug resistance.

The invention discloses a bacteriostatic composition for controlling staphylococcus aureus pollution and application thereof, and relates to the field of food safety, the bacteriostatic composition comprises a bacteriostatic part and a synergist; the antibacterial part is aminoglycoside antibiotics and / or disinfectants, and the synergist is 4-nitrobenzaldehyde. The 4-nitrobenzaldehyde provided by the invention can obviously enhance the bacteriostatic activity of various aminoglycoside antibiotics (such as gentamicin, kanamycin, tobramycin, neomycin and streptomycin) and common disinfectants (such as hydrogen peroxide, nisin and sodium hypochlorite) on staphylococcus aureus, and has broad-spectrum synergistic capacity.