40 results about "Corneal limbus" patented technology

An incisional instrument and method of use for creating accurate, reproducible surgical incisions. An exemplary embodiment includes an incisional instrument configured for attachment to a patient's eye and for use performing arcuate limbal relaxing incisions (LRIs). The incisional instrument is made up of two coaxial, interconnecting pieces: a docking piece and a cutting piece. The docking piece includes a suction mechanism and is configured for being secured to a patient's eye just outside the corneal limbus. The cutting piece is configured to fit flush within the docking piece and includes cutting blades and one or more handles for rotating the cutting piece relative to the docking piece. When assembled, the cutting blades extend into a patient's eye a desired LRI depth. An embodiment further includes an arcuate guide template with stoppers for providing lateral, arcuate stops for precise cuts in the desired LRI locations. An LRI method includes the steps of utilizing the incisional instrument in an ophthalmologic LRI procedure.

The invention provides an eye red grade analysis method and device combined with blood vessel morphological analysis and a medium, and relates to the technical field of image processing, the method comprises the following steps: inputting a target eye image into a pre-trained segmentation model to obtain a conjunctiva region and a cornea region, determining a corneal limbus region based on the conjunctiva region and the cornea region, and determining the corneal limbus region based on the cornea limbus region; extracting a red pixel region in the conjunctiva region and the corneal limbus region, performing skeletonization processing to obtain a skeletonized mask image, expanding the skeletonized mask image, and performing XOR operation on the skeletonized mask image and the red pixel region to obtain a bleeding region; determining a blood vessel region in the red pixel region based on the hemorrhage region and measuring a blood vessel shape value, determining an eye red level based on an area ratio of the red pixel region in the conjunctival region and the limbal region, and further correcting the eye red level through the blood vessel shape value. The method can accurately separate the blood vessel and determine the eye red grade in combination with the blood vessel form, and eye red analysis is more comprehensive and scientific.

The invention belongs to the technical field of cytobiology and regenerative medicine, and particularly relates to a porcine-derived corneal limbus epithelial stem cell culture method and application, during culture, pretreatment is firstly carried out, then a culture dish is coated, cells are cultured and passaged after centrifugation, dryness identification is carried out through cell immunofluorescence color, and the porcine-derived corneal limbus epithelial stem cells are obtained. The stem cell engineering vector can be applied to damage repair of corneal diseases including corneal limbal stem cell deficiency, during repair, the stem cell engineering vector is compounded with a biological material to form the stem cell engineering vector, the stem cell engineering vector is implanted into a damaged part, the separation and culture method is simple in step, the cell obtaining amount is large, and the stem cell engineering vector is suitable for being separated from eyeballs of a large animal-pig; the corneal limbus epithelial stem cells cultured in vitro can form cell clusters called stem cell islands, the obtained corneal limbus epithelial stem cells have phenotypes of BMI1 + and delta Np63 alpha +, and CK14 + and ABCG < 2 + >, and a new way can be provided for cell transplantation based on experimental research and clinical research.

A tomographic imaging system, methods to visualize tomographic images, and a ‎stereoscopic calibration procedure for obtaining two-dimensional cross-sectional ‎and three-dimensional volumetric images over the entire anterior eye. The optical ‎system combines stereoscopic imaging with patterned illumination (e.g., slit-‎scanning or array of slits). The captured cross-sectional images can be viewed ‎individually or as a fly-through video. The calibration and reconstruction methods ‎determine the true spatial location of imaged features and record a 3D volumetric ‎representation of the anterior eye. The system can image the entire anterior ‎chamber and contiguous structures, including the corneal limbus, sclera and ‎eyelids. Furthermore, the system can obtain corneal topography. ‎

A method and device are disclosed for determining the position of a flap incision on a cornea and the position of the hinge associated with the flap. The method comprises the steps of: capturing an image of an eye, including at least the pupil and the limbus; determining a region including the outer circumference of the flap incision; determining the position of the flap incision; determining a straight region including the hinge; and determining the position of the hinge.

Glaucoma can be treated by implanting an intraocular shunt in the eye. The implantation can be performed by first determining an entry area below a corneal limbus of an eye. Thereafter, the intraocular shunt can be inserted into eye tissue through the entry area such that an inflow end of the shunt is positioned in the anterior chamber of the eye and an outflow end of the shunt is positioned between layers of Tenon's capsule.

The present disclosure relates to intraocular implants for treating a condition of the eye, wherein the implant is configured to deliver a drug to the eye. The present disclosure also relates to methods of treating a condition of the eye by delivering a drug from intraocular implants to the posterior chamber (e.g., sulcus), iridocorneal angle, sclera, cornea, limbus, and vitreous.

The invention discloses an in-vivo cornea biomechanical characteristic measuring device and application, and belongs to the technical field of ophthalmology biomechanical measurement, the in-vivo cornea biomechanical characteristic measuring device comprises a negative pressure adsorption module and an imaging module; wherein a single-side opening groove is formed in the negative pressure adsorption module; the open side of the open slot is adsorbed on the limbus of the cornea; the open slot and the front surface of the cornea form a closed cavity; the closed cavity is used for receiving external negative pressure, so that the cornea is stressed to expand; the external negative pressure is monotonically changed and is changed once every preset time, so that the cornea stress is changed and the morphology is changed; by means of the designed negative pressure adsorption module, stable pressurization of external negative pressure on the closed cavity instead of instantaneous pressure can be achieved, so that the cornea is stably stressed to generate morphology changes, the stable morphology is kept instead of the transient morphology, and the influence of cornea inertia and viscoelasticity is effectively avoided; and accurate measurement of the static biomechanical characteristics of the cornea can be realized in a non-invasive manner.

Systems and methods for determining IRA candidacy and optimizing autologous corneal graft location and orientation to standardize and improve IRA procedures. A novel digital virtual model (i.e., a digital twin) of the eye is provided that integrates corneal imaging, pachymetry, topography and limbus / white-to-white measurements, and automates key surgical variables such as trephine size, autologous corneal graft rotations, and curvature and thickness matching, reducing cognitive load on the surgeon and improving accuracy. The embodiments herein provide precise graft placement parameters to maximize the amount of clear cornea in the visual axis. By providing real-time, patient specific guidance, the present embodiments enhance surgical precision and visual outcomes.

The utility model belongs to the technical field of ophthalmologic surgical instruments, and particularly relates to an ophthalmic sclera positioner which comprises a first ruler arm and a second ruler arm. The middle part of the second ruler arm is hinged with the middle part of the first ruler arm; the positioning part is arranged at the bottom end of the first ruler arm; the marking part is detachably connected to the bottom end of the second ruler arm; the ruler is fixedly connected to one side of the first ruler arm, and the scale surface of the ruler corresponds to the second ruler arm; and the driving part is arranged on the first ruler arm and is in transmission connection with the second ruler arm. According to the utility model, the positioning part coincides with the corneal limbus exposed under the visual field, so that the accuracy of the measuring direction can be ensured; the required position is measured by changing the reading on the scale, after the required position is obtained, dyeing marking is carried out on the sclera surface through the marking part, convenience and rapidness are achieved, and the problem that repeated operation is needed when the mark disappears can be solved. And the marking part is disposable, so that the accuracy and the convenience of sclera positioning are improved, and the operation safety is improved.

The utility model discloses a micropulse ciliary body photocoagulation assist device which comprises an auxiliary handle, an eyeball fixing ring is connected to the end of the auxiliary handle, fixing sawteeth used for assisting in fixing an eyeball are evenly distributed at the bottom end of the eyeball fixing ring, and the end of each fixing sawtooth is arranged to be an arc-shaped end. The outer surface of the eyeball fixing ring is fixedly connected with a probe auxiliary device used in cooperation with the micro-pulse probe, and the probe auxiliary device is arranged on the side portion of the auxiliary handle. The eyeball fixing ring is arranged at the corneal limbus of the surface of an eyeball, the fixing sawteeth make contact with the ocular surface and are slightly pressed to fix the eyeball, the micro-pulse probe penetrates through the probe auxiliary device to extend to the ocular surface, and the ciliary body photocoagulation operation is started to be carried out, and in the operation, the probe auxiliary device supports the micro-pulse probe and limits the moving route of the micro-pulse probe. Therefore, the ocular surface marking of the ciliary body photocoagulation does not need to be specially carried out, the operation time is shortened, and the probe control precision and the use safety are improved.

The invention belongs to the technical field of biomedical engineering, and particularly relates to human corneal limbus microenvironment cell exosome eye drops as well as a preparation method and application thereof. The invention discloses human corneal limbus microenvironment cell exosome eye drops. The unique active ingredient in the eye drops is human corneal limbus microenvironment cell exosome. The invention aims to provide eye drops containing human corneal limbus microenvironment cell exosomes. The eye drops are suitable for repairing corneal injuries caused by various factors clinically and relieving eye diseases such as visual fatigue, xerophthalmia, corneal limbus stem cell deficiency and immune lesions caused by graft-versus-host diseases.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The application discloses application of a norepinephrine inhibitor in preparation of a medicine for treating or relieving corneal limbal stem cell deficiency, and belongs to the technical field of medicines.The experiment proves that sympathetic nerves are excessively activated in a mouse model of corneal limbal stem cell deficiency, and the release of norepinephrine is increased, and the experiment proves that 6-hydroxydopamine hydrochloride can destroy sympathetic nerves to inhibit the release of norepinephrine, and a tyrosine hydroxylase inhibitor can inhibit the synthesis of norepinephrine, and both can further relieve the symptoms of corneal limbal stem cell deficiency.The norepinephrine inhibitor can improve the corneal limbal microenvironment, reduce corneal neovascularization, reduce corneal epithelialization, enhance corneal transparency and relieve corneal edema, and provides a new direction and a new way for the pathogenic mechanism and intervention target of corneal limbal stem cell deficiency, and has a wide application prospect.

The invention provides an astigmatism axial position marker and a marking method, and relates to the technical field of medical instruments. The astigmatism axial position marker comprises a base, a control panel, a base and a display panel, the control panel is arranged in the front of the center of the upper end face of the base, the base is arranged in the rear of the center of the upper end face of the base, the display panel is arranged in the center of the front end face of the base, and a body is arranged on the upper end face of the base. A storage groove is formed in the upper center of the rear end face of the body. Facial feature points are extracted through image processing, the distance between the two eyes of a patient is calculated, the positions of the two flexible eyeshades are adjusted through the adjusting structure, the flexible eyeshades are made to be rapidly matched with the distance between the two eyes of the patient, and therefore it is ensured that the two monitoring marking structures can accurately detect and mark the cornea or a target area, and the detection accuracy is improved. Marking content can comprise specific reference marks (such as 0 degree, 180 degrees and 270 degrees) of corneal limbus or the axial position of an intraocular lens to be implanted and the like.

Capsid protein mutants capable of increasing the ability of AAVs to infect ocular tissue and the use thereof. A Cap mutant is obtained by replacing an amino acid sequence of an AAV capsid protein which is homologous to amino acids at positions D561 to R588 of a wild-type AAV2 capsid protein with one of the polypeptides as shown in SEQ ID NOs. 6-39. Some Cap mutants can infect a mouse retina from the RGC layer, cone and rod cells of INL and ONL, corneal limbus regions, trabecular meshwork, RPE and a small amount of RPE cells by administration via intravitreous injection, can solve the problem that it is difficult for the conventional serotype AAV to infect the cells in the posterior layer of the retina due to insufficient penetrability, and is expected to become a potential carrier for the treatment of glaucoma. Experimental data show that the abilities of some Cap mutants to infect and penetrate the retina of non-human primates are optimized, thereby providing a new tool for developing gene therapy preparations for ophthalmic diseases or constructing animal models related to ophthalmic diseases.

The invention relates to application of regulatory T cells and mesenchymal stem cells in preparation of medicines or medical instruments for repairing corneal nerve injury. The regulatory T cells are prepared from peripheral blood mononuclear cells, the adipose-derived stem cells are prepared from adipose tissues, the sources are wide, the adipose-derived stem cells are easy to obtain, the biocompatibility is good, the quantity is large and the purity is high after in-vitro extraction and amplification, the regulatory T cells and the adipose-derived stem cells are combined for use, corneal nerve repair can be promoted, and the treatment and repair effect is excellent. Further verification shows that corneal nerve repair is beneficial to corneal epithelium defect repair, corneal edema reduction and inhibition of generation of neovascularization and lymphatic vessels, and especially has a good application prospect in the aspect of corneal epithelium damage repair involving corneal limbus.

An anterior chamber maintainer (ACM), which is to be utilized in connection with maintaining physiological intraocular pressure during intraocular surgery, is disclosed and comprises an axially elongated tubular member which is adapted to be fluidically connected at a first end to a supply of balanced salt solution (BSS) for introduction into the anterior chamber of an eye so as to maintain adequate intraocular pressure while performing any intraocular surgery. A second opposite end of the anterior chamber maintainer (ACM) is provided with a sharply pointed end for insertion through a paracentesis incision formed within the limbus region of the eye, and a non-deformable, incompressible, tapered, annular bulbous portion is interposed between the first and second ends such that as the second end passes through the paracentesis incision, the non-deformable, incompressible, tapered, annular bulbous portion will cause the paracentesis incision to deform or expand radially outwardly, and after passing through the paracentesis incision, the non-deformable, incompressible, annular bulbous portion will trap the anterior chamber maintainer (ACM) within the anterior chamber of the eye.

The invention belongs to the technical field of biology, and particularly relates to a cytoskeleton for promoting proliferation of corneal limbal stem cells. The raw materials of the cytoskeleton provided by the invention are chitosan and sodium hyaluronate, and the cytoskeleton has good biocompatibility and biological activity, can simulate the characteristics of an extracellular matrix, provides necessary physical and chemical support for stem cells, and does not cause immune response or cytotoxicity. The addition of the magnetic nanoparticles further enhances the performance of the cytoskeleton. Directional arrangement under the action of a magnetic field can provide structural support closer to an in-vivo microenvironment for cells, and adhesion and proliferation of the cells are promoted. The cell scaffold provided by the invention can provide a good growth microenvironment for the corneal limbus stem cells, and is beneficial to solving the problem that the corneal limbus stem cells are easy to lose activity and function in in-vitro culture.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The present disclosure relates to intraocular drug-eluting particulate implants and dry particulate formulations comprising a plurality of drug-eluting particulate implants for use in the treatment of conditions of the eye, wherein the implants are configured to deliver a drug to the eye without a carrier. The present disclosure also relates to methods of treating conditions of the eye by delivering one or more drugs from a drug eluting implant or dry particulate formulation to the anterior chamber, posterior chamber (e.g., ciliary sulcus), iris corneal horn, sclera, cornea, limbus, subconjunctival space, subcapsular space, and vitreous body.

Use of a recombinant transforming growth factor beta induced (TGFBI) protein in lattice corneal dystrophy (LCD) is provided, belonging to the technical field of biomedicine. Use of a recombinant TGFBI protein and a recombinant TGFBI protein-expressing reagent in preparation of a medicament for treating LCD are provided. It is confirmed that dropping eye drops of the recombinant TGFBI protein can promote the repair of corneal epithelial damages in an LCD mouse model; corneal intrastromal injection of the recombinant TGFBI protein can promote the repair of corneal epithelial damages in the LCD mouse model; and limbal intrastromal injection of an adeno-associated virus (AAV) vector carrying a Tgfbi gene can promote the repair of epithelial damages in a TGFBI point mutation-carrying mouse. Therefore, the recombinant TGFBI protein-related reagents exhibit a potential to treat the LCD.

The invention relates to a construction method of tissue engineering corneal epithelial cells. The construction method comprises the following steps: obtaining corneal limbus tissue blocks; digesting the tissue block, and separating to obtain epithelial cells of the tissue block; the method comprises the following steps: attaching epithelial cells to ultrathin amniotic membrane-umbilical cord derived mesenchymal stem cells, culturing in an SHEM culture medium, changing the SHEM culture medium once every two days, culturing for a period of time, and culturing the epithelial cells for a period of time by using a gas-liquid interface culture method to promote the multi-layer growth of the epithelial cells, thereby obtaining the tissue engineering corneal epithelial cells. Through a controllable preparation scheme, the amniotic membrane carrier with a certain thickness is obtained so as to increase the transparency of the amniotic membrane carrier, meanwhile, the matrix surface of the amniotic membrane carrier is inoculated with MSC, the healthy corneal limbus matrix microenvironment of a human body is simulated, damaged corneal limbus tissue / corneal epithelium is better repaired or regenerated, and the ocular surface microenvironment is improved. The produced ultrathin amnion combined mesenchymal stem cell carrier can obviously improve the effect of a tissue engineering corneal epithelium transplantation operation.

The utility model discloses a novel vitreous cavity injector capable of being positioned, which comprises a guiding and positioning mechanism and an injector, the guiding and positioning mechanism comprises an outer cylinder and a positioning ring, the positioning ring is arranged at the front end of the outer cylinder, the positioning ring and the outer cylinder are of an integrated structure which is concentrically arranged, and a concentric annular silica gel gasket is arranged on the front end face of the positioning ring. The silica gel gasket is positioned on the flat part of the ciliary body behind the cornea sclera edge of the eyeball of the patient; and a metal needle catheter is arranged in the center of the front end surface of the outer cylinder. The silica gel gasket of the positioning ring at the front end of the outer cylinder is tightly attached to the cornea sclera edge and makes flexible contact with the eyeball, and the needle head can be directly positioned on the flat portion of the ciliary body of a patient; after the needle head is inserted into the eyeball of a patient, the positioning ring at the front end of the outer cylinder provides certain supporting force, so that unnecessary damage to the eyeball of the patient caused by shaking of the hand, slippage of the needle head and the like during medicine injection is prevented; the positioning ring is suitable for the outer diameter radians of various eyeballs, and can accurately position a 4 mm injection point behind the corneal limbus.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The invention discloses an intraoperative extracorporeal Schlemm tube positioning method and application of the intraoperative extracorporeal Schlemm tube positioning method in a glaucoma surgery, and the intraoperative extracorporeal Schlemm tube positioning method comprises the steps that a conjunctival flap with a dome as a base is manufactured, a full-thickness bulbar conjunctiva is cut off along the corneal limbus radian through a conjunctival shear, and the cut-off length is 8 + / -2 mm; performing blunt separation by clinging to the sclera surface to form a rectangular sclera area; the method comprises the following steps: manufacturing a superficial sclera flap taking limbus corneae as a substrate, forwards sectioning a sclera to a transparent cornea area, placing a mitomycin cotton piece under the sclera flap, staying for 3 minutes, and flushing; a light ring and a shadow ring are observed to be formed on the whole corneal limbus by means of irradiation of the positioning optical fiber device, and lineation marking is carried out; carrying out 1mm * 2mm deep sclera flap sectioning in the direction from 1mm behind the marking line to the limbus corneae until the limbus corneae is transparent; in the sectioning process, 0.3 mm-0. 5 mm in front of the marking line is the rear edge of the outer path Schlemm tube, the outer wall of the outer path Schlemm tube can be opened by conducting conventional fine sectioning on the outer path Schlemm tube at the position, positioning is accurate, and the surgical success rate can be improved.

A contact lens for application in practice of orthokeratology on an eye, including a curved shell having a concave surface and a convex surface. The concave surface includes a carrier zone and a back shaping zone, the back shaping zone having a first curvature and the carrier zone having at least one second curvature. The curved shell has a geometric center and the back shaping zone has a shaping zone center and the back shaping zone center is offset peripherally from the geometric center. The curved shell can have an overall diameter that approximates a corneal limbal diameter of the eye to which the contact lens is to be applied.

The present invention relates to an artificial cornea, comprising a cornea body, an inner support ring, several stress wires and an outer support ring; the cornea body is provided with a sclera at the periphery, the outer support ring is provided at the periphery of the sclera, and the inner circumference of the outer support ring is preset in the outer ring layer of the sclera, the cornea body is provided with an inner support ring in the middle, and the middle of the inner support ring is a central optical part; the inner end of the stress wire is connected to the outer edge of the inner support ring, radially extends outward, and is buried in the outer layer of the cornea body, and finally passes through the interlayer of the sclera and its outer end is connected to the inner circle of the outer support ring. The artificial cornea of ​​this case uses prestress to share the intraocular pressure of the central optical part of the artificial cornea to the broad corneal limbal tissue, so as to avoid the local artificial cornea from compressing the original corneal tissue under long-term pressure, thereby enhancing the stability of the artificial cornea in the eye.

The invention provides a biological amnion cornea surface transplantation device which comprises a fixing body which is a circular ring and is matched with the corneal limbus of a human body. The fixing support piece at least comprises a fixing loop, and the fixing loop is a metal wire made of shape memory metal; one end of the fixing loop is fixedly connected with the fixing main body, and the other end of the fixing loop is far away from the diameter direction of the fixing main body passing through the connection point of the fixing loop and the fixing main body; and at least two fixing support pieces are arranged on the periphery of the fixing main body. Deformation and recovery of the memory metal and structural characteristics of human eyeballs are ingeniously utilized, the fixing loops are clamped to penetrate through the amniotic membrane and the corneal limbus conjunctiva and then are loosened, the memory metal recovers deformation in the gap between the conjunctiva and the sclera, the at least two fixing loops form a clamping jaw in the gap between the conjunctiva and the sclera along the corneal limbus radian, and the clamping jaw is clamped by the memory metal. Therefore, the amniotic membrane is pressed on the surface of the cornea by the fixing main body. The device is simple in structure, low in cost and easy to operate, trauma in an operation is small, and postoperative recovery is fast.