Method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes

An electrospinning process and hybrid fiber technology, which is applied in the field of tissue engineering limbus construction based on electrospinning hybrid fiber membranes, can solve the difficulty in regulating and controlling the Wiener structure of hydrogels, it is difficult to obtain a 3D structure, and it is difficult to perform fine regulation. and other problems, to achieve the effect of easy control, easy control and easy implementation of process conditions and mixed fiber membrane

Inactive Publication Date: 2019-04-02
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

However, it is difficult to obtain a 3D structure by using planar membranes such as amniotic membrane as the base to culture cells, and the Wiener structure of t

Method used

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  • Method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes
  • Method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes
  • Method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes

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Embodiment 1

[0042] see Figure 4 , Figure 5A and Figure 5B According to the first embodiment of the present invention, the method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membrane mainly includes the following steps:

[0043] (1) Take a certain mass of polycaprolactone (PCL) / gelatin (gelatin), dissolve PCL and gelatin in a certain volume of trifluoroethanol (TFE) and glacial acetic acid, and stir overnight on a magnetic stirrer A PCL / gelatin mixed solution was obtained. Preferably, the mass ratio of the PCL and the gelatin is 8:2.

[0044] (2) The mixed solution obtained in step (1) is packed into a syringe with a volume of 10ml, the front end of the syringe is equipped with a 19G needle, the receiving end of the electrospinning machine is covered with 33cm*34cm tin foil, and the distance between the positive and negative electrodes is 18cm. The injection speed is 0.5ml / h, the high voltage power supply is turned on, and the positive and...

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Abstract

The present invention belongs to the technical field of tissue engineering and discloses a method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes. Themethod comprises the following steps: (1) mixed fiber membranes are prepared by using an electrospinning technology, and by controlling parameters of electrospinning to regulate and control spinningshapes, spinning diameters and spinning assembly methods, the fiber membranes with a micro-nano structure conductive for cell growth are obtained; (2) amphiphilicity and degradation cycles of the mixed fiber membranes are adjusted by controlling crosslinking degrees of mixed spinning; and (3) Matrigel is wrapped onto surfaces of fiber filaments, corneal limbal stem cells and corneal limbal microenvironment cells are planted in gaps, and the tissue engineering corneal limbus is further cultured and obtained. The method has strong scientific and technological innovation, and very strong medicalprospects, and solves a limitation that corneas are currently provided only by living body.

Description

technical field [0001] The invention belongs to the technical field related to tissue engineering, and more specifically relates to a method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes. Background technique [0002] Limbal stem cell transplantation is currently the main treatment method for limbal stem cell decompensation (LSCD). For patients, it is difficult to extract a sufficient number of limbal stem cells from the autologous cornea. This requires obtaining some limbal stem cells from sources such as autologous, allogeneic, relatives, or cadavers, expanding them in vitro, using various delivery devices to culture limbal stem cells, and then replanting them in patients. Providing a three-dimensional structure that allows limbal stem cells to grow better and resembles the human limbus could improve the quality of the graft for better therapeutic outcomes. [0003] At present, researchers in tissue engineering use amni...

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Application Information

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IPC IPC(8): C12N5/079C12N5/0797
CPCC12N5/0621C12N5/0623C12N2513/00C12N2533/30
Inventor 李阳王平高晴琴李贵刚王玮蒋林王云明周华民黄志高张云
Owner HUAZHONG UNIV OF SCI & TECH
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