The invention discloses a preparation method of a rat
corneal limbus stem cell deficiency model in the field of
corneal epithelium. The method comprises the following steps of S1, anesthetizing an experimental rat by adopting pentobarbital
sodium intraperitoneal injection, and dripping 4 mg / mL of
procaine into the experimental eye of the rat for
local anesthesia; S2, by utilizing conical
filter paper, infiltrating all parts of the
corneal limbus of the rat at the same time, and controlling the placement time of the
filter paper more accurately; and S3, soaking the conical
filter paper prepared in the step S2 in a
sodium hydroxide solution, clamping the top of the conical filter paper through
tweezers, and slightly sucking redundant liquid on the conical filter paper through gauze. By utilizing the conical filter paper, all the parts of the
corneal limbus of the rat can be infiltrated at the same time, and the placement time of the filter paper can be more accurately controlled; compared with a traditional plane filter paper method, the preparation method has the advantages that the rat corneal limbus
stem cell deficiency can be more stably and reliably induced, a good
animal model for simulating a clinical patient is provided for
experimental research, and a foundation is laid for further therapeutic research.