44 results about "Corneal neoplasm" patented technology

The present disclosure describes mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. The ELSCs of the present disclosure are capable of proliferating in an in vitro culture, maintain the potential to differentiate into cells of endoderm, mesoderm, and ectoderm lineage in culture, and are capable of forming embryoid-like bodies when placed in suspension culture. Thus, these cells possess multi-lineage differentiation potential and self-renewing capability. ELSCs may be a promising therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries.

The present disclosure describes a tissue system with self-regenerating limbal stem cells, wherein the limbal stem cells are primarily undifferentiated stem cells (USCs). The tissue system is derived from isolated corneal limbal tissue, and is suitable for restoring ocular surface impairments, particularly those that result from limbal stem cell deficiencies. The tissue system is generated by selectively augmenting the tissue system for USCs, for example by selecting and sorting cells that express stem cell-specific surface markers, such as stage specific embryonic antigen marker 4 (SSEA-4). After isolation, the USCs are cultured on a tissue base in the presence of enriched medium to generate the tissue system, which is suitable for transplantation, implantation, or grafting.

The invention relates to a mobile medical device, in particular to a strabismus diagnosis device based on a mobile platform. The strabismus diagnosis device based on the mobile platform comprises an image input device, an image pretreatment device, an image smoothing and gray level conversion processing unit, an ocular region positioning device, a corneal limbus positioning device, a reflection point positioning device, an offset calculation device and an image output device, wherein the image input device is used for obtaining an ocular region image, and the image pretreatment device also comprises an image resolution regulation image. On the basis of the mobile platform, the strabismus diagnosis device based on the mobile platform can be installed on the intelligent mobile equipment, such as a smart phone and a tablet personal computer, the strabismus detection can be conveniently carried out, and the operation can be conveniently carried out.

The invention relates to a preparation method and application thereof of an acellular conjunctiva matrix used as a tissue engineering corneal scaffold material. The method comprises the following steps: removing cell components in a bulbar conjunctiva first; preparing the acellular conjunctiva matrix; and taking the acellular conjunctiva matrix as a tissue engineering corneal scaffold. A rabbit corneal epithelium or an endothelial cell can form a good cell single layer on the scaffold, can construct a tissue engineering corneal epithelium or endothelium, and can successfully transplant the tissue engineering corneal epithelium or endothelium onto rabbit animal model eyes. The degradation time of the built tissue engineering cornea is longer, rabbit eyes have no obvious immune reject reaction, and the therapeutic effects on the lack of corneal limbus stem cells or the decompensation of the corneal endothelial cell function are obvious. The method and the application thereof have the advantages of simple preparation method, good biocompatibility, low antigenicity, easy growth and proliferation of seed cells, slow degradation, extensive bulbar conjunctiva sources and wide application and development prospects; and the transparency can be maintained all the time in the training process and after transplantation.

The invention provides a cornea metaphase preservation solution, and preparing and using methods thereof. The cornea metaphase preservation solution is a cell culture minimum essential medium (MDM) with chondroitin sulfate, low molecular dextran, L-glutamine, dexamethasone, tobramycin, 2-hydroxyethyl and Y-27632 added. The preservation solution not only can keep activity and normal morphology of cornea endothelial cells, but also can enhance viability of corneal limbus epithelial cells and improve clone ability of corneal limbus stem cells. And especially, a medium to long term preservation effect is obvious, phenomena of deformation, conjugation and the like of endothelial cells of a control group cornea do not appear in long term preservation, endothelial morphology of the control group cornea is consistent with endothelial morphology of a cornea preserved for 4 days, and the endothelial cells of the control group cornea are still regular and few in cell conjugation phenomena. The preservation solution can effectively prevent cell apoptosis phenomena during the process of preserving isolated cornea materials, increases activity and clone forming ability of the corneal limbus stem cells, and enables the cornea to maintain a transparent feature in a long preservation time.

The present disclosure describes the generation of neural cells and neurons from mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. Specifically, the present disclosure describes the generation of neuroprogenitor cells and differentiated dopaminergic neurons from ELSCs. The disclosed methods demonstrate the potential of ELSCs as a therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries. Neuroprogenitor cells generated from ELSCs isolated from corneo-limbal tissue were transplanted into a rat model of Parkinson's disease, and were able to alleviate motor abnormalities in the rats for at least six months.

The invention relates to a method for preparing an amniotic compound corneal limbus stem cell membrane, which comprises the following steps of: firstly, preparing a sterile amniotic membrane with epithelium removed, placing the epithelial surface of the amniotic membrane downwards on an end face of a sleeve made of a poly propylene material, and covering an embedded culture transwell on the amniotic membrane of the sleeve to obtain an amniotic-membrane-embedded culture mold; placing the culture mold in holes in a 6-hole plate on which a mouse embryonic fibroblast cell feeder layer is spread; and finally, preparing a corneal limbus stem cell suspension by using a digestion method, inoculating the suspension on the epithelial surface of the amniotic membrane, inoculating 2.0-3.0*10<5> corneal limbus stem cells for each culture mold, and culturing the corneal limbus stem cells for 10 days to obtain the amniotic compound corneal limbus stem cell membrane. Compared with the traditional amniotic membrane spreading method and the latex ring amniotic membrane fixing method, the amniotic compound corneal limbus stem cell membrane prepared with the method has flat amniotic membrane culturing surface and uniform corneal limbus stem cell stratified layer and especially has the advantage of convenience for clinic application and difficulty in rapture of the amniotic membrane.

The present invention includes an ophthalmic device and system of mounting for correcting hyperopia, myopia and presbyopia. The present invention includes a limbus ring mountable in an encircling relation to a central optic zone of a cornea on a limbus annulus surrounding the cornea. In the limbus ring defines a substantially annular toroid defining a first average diameter that is selectable and has a hydrophilic coating disposed thereon. The inner radius of the limbus ring is selectable such that, upon mounting on the limbus annulus, the limbus ring causes the limbus annulus to contract or expand thereby causing the curvature of the cornea and the eye length to increase or decrease. The mounting system of the present invention is adapted to receive a limbus ring and further adapted to selectively place the limbus ring on a limbus annulus.

The invention relates to the field of bioengineering medical materials, and discloses decellularized corneal limbus protective liquid and a preparation method of decellularized corneal limbus. The component of the decellularized corneal limbus protective liquid is a PBS buffer solution containing hyaluronic acid of 5 to 12 g/L, chondroitin sulfate of 5 to 20 g/L, low molecular dextran of 3 to 10 g/L and tobramycin of 2.5 to 5 mg/L. A fresh pig corneoscleral limbal matrix containing lamina elastica anterior is sealed in the decellularized corneal limbus protective liquid and cells are crushed through high hydrostatic pressure treatment; the corneal limbus of the crushed cells are put into the decellularized corneal limbus protective liquid added with a detergent and nuclease to perform oscillation decellularized treatment; and the decellularized corneal limbus is put into the decellularized corneal limbus protective liquid to be rinsed to finish preparation. The decellularized corneal limbus obtained by the method is complete in decellularization, minimally reduces damage to the corneal limbus structure, has high biocompatibility and is favorable for autologous cell proliferation and crawling.

The invention discloses a preservation method for corneal limbus tissue. The method comprises a step of disinfecting fresh corneal limbus tissue and a step of air exposure preservation of the disinfected fresh corneal limbus tissue. The step of air exposure preservation is as follows: the fresh corneal limbus tissue is placed on a permeable covering film, preservation liquid or a cell culture medium capable of providing nutrients to the corneal limbus tissue is arranged below the covering film, epitheliums of cornea directly contact with air, the temperature of a culture environment in the step is 0 to 37 DEG C, and oxygen concentration is 0 to 20%. Compared to immersion preservation in the prior art, the preservation method provided in the invention has the following advantages: the structure of the preserved corneal limbus tissue is more integral; obvious apoptosis of the epitheliums of cornea does not occur; stem cells of corneal limbus can maintain high activity; and process flow is simple and reliable, is easy to implement, costs little and has other efficacy.

The invention publishes a kind of method to stores the stem cell of corneal limbus by constructing planting piece of corneal epithelium without feeder layer. stores the stem cell of corneal limbus by constructing planting piece of corneal epithelium without feeder layer. It can stores self stem cell of corneal limbus for long time in order to satisfy the request of transporting, storing and proliferating stem cell of corneal limbus under different experimental conditions. The invention stores the stem cell of corneal limbus by constructing planting piece of corneal epithelium without feeder layer, to make that corneal epithelium rebuild self when stem cell of corneal limbus colobomas come true in clinic. The invention can successfully form planting piece of artificial corneal epithelium which can autoplastic graft to misshapen surface of cornea of corneal limbus stem cell and the effect to get eyesight back is obvious. So it has a wide application potency in clinic.

The invention discloses an ophthalmic operation ultrasonic scalpel which comprises a handle and a tool bit. The handle is provided with a shell, a power source and an ultrasonic generating device arearranged inside the shell, and the head end of the shell is provided with the output end of the ultrasonic generating device; the tool bit is provided with a tool bit body, the tool bit body is sequentially provided with a connecting part, a limiting installing part and a blade part from bottom to top, the connecting part is used for being detachably connected with the output end of the ultrasonicgenerating device, the limiting installing part is used for installing a limiting mechanism to ensure the cutting depth, and the blade part is used for cutting. The purpose that a blade can instantlycut corneas downwards is achieved, and the scalpel can be used for keratoplasty and corneal limbus dissociation dissection.

Methods and systems for overlay imaging for registration of a patient eye for laser surgery include aligning and overlaying a surgical image of the limbus over a previously acquired diagnostic image of the limbus. The surgical image is displayed with a degree of transparency and enabled to rotate about the limbus center. Various types of colors, markers, contrast, and backgrounds may be used to generate a display for determining the alignment angle between the diagnostic image and the surgical image.

The invention discloses RNCEC (rabbit normal corneal epithelial cells) and an application thereof. The cell is named as RNCEC/HL-032, and the collection number is CCTCC NO: C201677. A primary isolation culture method of the cell is as follows: a normal corneal limbus tissue sample of a New Zealand rabbit is digested, then dispase and DNase I are added after digestion, cells are filtered and collected in a centrifugal manner and then resuspended with an HL culture medium for inoculated culture. The subculturing method of RNCEC comprises steps as follows: the cells are digested with pancreatin-EDTA when proliferating to have the abundance of 70%-90% and then neutralized with a DMEM (dulbecco's modified eagle medium); the cells are collected in a centrifugal manner and resuspended with the HL culture medium for inoculated culture. A 2D and 3D culturing system established for the cell can be used for animal substitutive experiments of corneal drugs and used for detecting pharmacology and toxicology of drugs and establishing a new experimental animal substitution model for corneal drug research.

The present invention belongs to the technical field of tissue engineering and discloses a method for constructing tissue engineering corneal limbus based on electrospinning mixed fiber membranes. Themethod comprises the following steps: (1) mixed fiber membranes are prepared by using an electrospinning technology, and by controlling parameters of electrospinning to regulate and control spinningshapes, spinning diameters and spinning assembly methods, the fiber membranes with a micro-nano structure conductive for cell growth are obtained; (2) amphiphilicity and degradation cycles of the mixed fiber membranes are adjusted by controlling crosslinking degrees of mixed spinning; and (3) Matrigel is wrapped onto surfaces of fiber filaments, corneal limbal stem cells and corneal limbal microenvironment cells are planted in gaps, and the tissue engineering corneal limbus is further cultured and obtained. The method has strong scientific and technological innovation, and very strong medicalprospects, and solves a limitation that corneas are currently provided only by living body.

The invention discloses a corneal limbus stem cell, and a culture method as well as application thereof. The culture method of the corneal limbus stem cell comprises the following steps: acquiring a proliferative corneal limbus tissue; adding the proliferative corneal limbus tissue into an MEM culture medium containing FBS and Ca<2+>, and culturing, wherein the concentration of the Ca<2+> in the MEM culture medium containing the FBS and the Ca<2+> is 0.01mmol/L to 0.09mmol/L. According to the culture method of the corneal limbus stem cell, disclosed by the invention, the differentiation of the corneal limbus stem cell can be inhibited, the generation of other cells caused by the differentiation of the corneal limbus stem cell is avoided, the purity of the corneal limbus stem cell obtained through culture is guaranteed, the degree of differentiation is lowered, and thus the effect of treating corresponding eye diseases through transplanting the corneal limbus stem cell is improved.

The invention discloses a preparation method of a rat corneal limbus stem cell deficiency model in the field of corneal epithelium. The method comprises the following steps of S1, anesthetizing an experimental rat by adopting pentobarbital sodium intraperitoneal injection, and dripping 4 mg/mL of procaine into the experimental eye of the rat for local anesthesia; S2, by utilizing conical filter paper, infiltrating all parts of the corneal limbus of the rat at the same time, and controlling the placement time of the filter paper more accurately; and S3, soaking the conical filter paper prepared in the step S2 in a sodium hydroxide solution, clamping the top of the conical filter paper through tweezers, and slightly sucking redundant liquid on the conical filter paper through gauze. By utilizing the conical filter paper, all the parts of the corneal limbus of the rat can be infiltrated at the same time, and the placement time of the filter paper can be more accurately controlled; compared with a traditional plane filter paper method, the preparation method has the advantages that the rat corneal limbus stem cell deficiency can be more stably and reliably induced, a good animal model for simulating a clinical patient is provided for experimental research, and a foundation is laid for further therapeutic research.

The invention discloses an eyeball movement distance and binocular movement consistency deviation detecting device and method based on a computer, relates to the field of medical instruments and aims to solve the problem that existing methods cannot quickly and accurately detect eyeball movement distance and cannot detect the eyeball movement distance when eyeballs are blocked. A head fixing mechanism is used for fixing the head of a patient, and a camera is used for displaying binocular images of the patient in real time and sending the images to the computer; the computer builds a plane rectangular coordinate system according to a reference image, extracts corneal limbi in the reference image and each test picture, reduces a complete image of each test corneal limbus according to characteristics of two reference corneal limbi, respectively extracts reference points on two reference corneal limbus images and test points corresponding to the reference points in test corneal limbus images and finally calculates distances by which each test point moves relative to the reference points and binocular movement consistency deviation. The eyeball movement distance and binocular movement consistency deviation detecting device and method is suitable for assessing eyeball movement functions.

The invention discloses an ocular surface positioner. The whole positioner is dome-shaped, the center of the positioner is of a hollow circular ring, the periphery of the hollow circular ring is of regularly arranged fan-shaped blades, the fan-shaped blades are radially arranged at a fixed included angle, corneal limbus marking lines are marked on the fan-shaped blades, the distance between the corneal limbus marking lines and the central point of the hollow circular ring is 6.0mm, the curvature from the corneal limbus marking lines to the central point of the hollow circular ring is 50, and the curvature radius of each outward blade of each corneal limbus marking line is 12.0mm. In clinical work of ophthalmology department, when accurate positioning is needed when an artificial lens is sutured and fixed or a ciliary body is sutured, accurate measurement and positioning can be achieved through the ocular surface positioner, operation is easy and convenient, precision is high, the operation time can be greatly shortened, and very high practicability is achieved.

The invention relates to a corneal limbus stem cell transplanter. The corneal limbus stem cell transplanter comprises a negative pressure ring and a cutter bit; the negative pressure ring comprises ahandle, a suction ring, a rail and a sliding column; the sliding column is arranged on the rail; the cutter bit comprises a cutter blade and a main body; the cutter bit is connected with the sliding column arranged on the rail through a circular column, and the cutter bit cuts corneal limbus stem cell tissue along the junctional zone of cornea, conjunctiva and sclera by pushing the sliding column.The corneal limbus stem cell transplanter has the advantages that by arranging the suction rings with the different specifications and different tilting angles, the precise positioning of the cutterblade in a cutter blade shell, the distance between the cutter bit and the upper end face of the suction ring and the length of a cutter edge, the extractor can precisely cut a plurality sections of corneal limbus stem cell tissue with the length being adjustable, the thickness being 50 microns and the width being 3 mm in the junctional zone of cornea, conjunctiva and sclera. The transplanter is easy and safe to operate, the operation difficult is greatly reduced, the operation time is saved, the treatment effect of operations is improved, the treatment benefits are maximized, and the studyingand training period of an operator is shortened.