27 results about "Corneal neoplasm" patented technology

An incisional instrument and method of use for creating accurate, reproducible surgical incisions. An exemplary embodiment includes an incisional instrument configured for attachment to a patient's eye and for use performing arcuate limbal relaxing incisions (LRIs). The incisional instrument is made up of two coaxial, interconnecting pieces: a docking piece and a cutting piece. The docking piece includes a suction mechanism and is configured for being secured to a patient's eye just outside the corneal limbus. The cutting piece is configured to fit flush within the docking piece and includes cutting blades and one or more handles for rotating the cutting piece relative to the docking piece. When assembled, the cutting blades extend into a patient's eye a desired LRI depth. An embodiment further includes an arcuate guide template with stoppers for providing lateral, arcuate stops for precise cuts in the desired LRI locations. An LRI method includes the steps of utilizing the incisional instrument in an ophthalmologic LRI procedure.

The invention belongs to the technical field of cytobiology and regenerative medicine, and particularly relates to a porcine-derived corneal limbus epithelial stem cell culture method and application, during culture, pretreatment is firstly carried out, then a culture dish is coated, cells are cultured and passaged after centrifugation, dryness identification is carried out through cell immunofluorescence color, and the porcine-derived corneal limbus epithelial stem cells are obtained. The stem cell engineering vector can be applied to damage repair of corneal diseases including corneal limbal stem cell deficiency, during repair, the stem cell engineering vector is compounded with a biological material to form the stem cell engineering vector, the stem cell engineering vector is implanted into a damaged part, the separation and culture method is simple in step, the cell obtaining amount is large, and the stem cell engineering vector is suitable for being separated from eyeballs of a large animal-pig; the corneal limbus epithelial stem cells cultured in vitro can form cell clusters called stem cell islands, the obtained corneal limbus epithelial stem cells have phenotypes of BMI1 + and delta Np63 alpha +, and CK14 + and ABCG < 2 + >, and a new way can be provided for cell transplantation based on experimental research and clinical research.

A tomographic imaging system, methods to visualize tomographic images, and a ‎stereoscopic calibration procedure for obtaining two-dimensional cross-sectional ‎and three-dimensional volumetric images over the entire anterior eye. The optical ‎system combines stereoscopic imaging with patterned illumination (e.g., slit-‎scanning or array of slits). The captured cross-sectional images can be viewed ‎individually or as a fly-through video. The calibration and reconstruction methods ‎determine the true spatial location of imaged features and record a 3D volumetric ‎representation of the anterior eye. The system can image the entire anterior ‎chamber and contiguous structures, including the corneal limbus, sclera and ‎eyelids. Furthermore, the system can obtain corneal topography. ‎

Glaucoma can be treated by implanting an intraocular shunt in the eye. The implantation can be performed by first determining an entry area below a corneal limbus of an eye. Thereafter, the intraocular shunt can be inserted into eye tissue through the entry area such that an inflow end of the shunt is positioned in the anterior chamber of the eye and an outflow end of the shunt is positioned between layers of Tenon's capsule.

The invention provides a corneal limbus stem cell in-situ repair microneedle array patch and a preparation method and application thereof.The corneal limbus stem cell in-situ repair microneedle array patch comprises a biocompatible backing layer and a microarray layer arranged on the biocompatible backing layer, microneedles of the microarray layer are of a conical structure, the height is 100-300 micrometers, the vertex angle is smaller than 30 degrees, and the thickness of the microneedles of the microarray layer is 10-30 micrometers. And a nano-scale porous structure for loading cornea stem cell suspension is arranged in the cornea stem cell support. Through precise geometric design, biomimetic material selection and spatial arrangement optimization, in-situ delivery, microenvironment activation and tissue fusion in a corneal limbus area are realized under the condition of not depending on in-vitro amplification and surgical suture, and the method becomes an important direction for promoting LSCD minimally invasive treatment strategy innovation.

The utility model belongs to the technical field of ophthalmologic surgical instruments, and particularly relates to an ophthalmic sclera positioner which comprises a first ruler arm and a second ruler arm. The middle part of the second ruler arm is hinged with the middle part of the first ruler arm; the positioning part is arranged at the bottom end of the first ruler arm; the marking part is detachably connected to the bottom end of the second ruler arm; the ruler is fixedly connected to one side of the first ruler arm, and the scale surface of the ruler corresponds to the second ruler arm; and the driving part is arranged on the first ruler arm and is in transmission connection with the second ruler arm. According to the utility model, the positioning part coincides with the corneal limbus exposed under the visual field, so that the accuracy of the measuring direction can be ensured; the required position is measured by changing the reading on the scale, after the required position is obtained, dyeing marking is carried out on the sclera surface through the marking part, convenience and rapidness are achieved, and the problem that repeated operation is needed when the mark disappears can be solved. And the marking part is disposable, so that the accuracy and the convenience of sclera positioning are improved, and the operation safety is improved.

The utility model discloses a micropulse ciliary body photocoagulation assist device which comprises an auxiliary handle, an eyeball fixing ring is connected to the end of the auxiliary handle, fixing sawteeth used for assisting in fixing an eyeball are evenly distributed at the bottom end of the eyeball fixing ring, and the end of each fixing sawtooth is arranged to be an arc-shaped end. The outer surface of the eyeball fixing ring is fixedly connected with a probe auxiliary device used in cooperation with the micro-pulse probe, and the probe auxiliary device is arranged on the side portion of the auxiliary handle. The eyeball fixing ring is arranged at the corneal limbus of the surface of an eyeball, the fixing sawteeth make contact with the ocular surface and are slightly pressed to fix the eyeball, the micro-pulse probe penetrates through the probe auxiliary device to extend to the ocular surface, and the ciliary body photocoagulation operation is started to be carried out, and in the operation, the probe auxiliary device supports the micro-pulse probe and limits the moving route of the micro-pulse probe. Therefore, the ocular surface marking of the ciliary body photocoagulation does not need to be specially carried out, the operation time is shortened, and the probe control precision and the use safety are improved.

The invention belongs to the technical field of biomedical engineering, and particularly relates to human corneal limbus microenvironment cell exosome eye drops as well as a preparation method and application thereof. The invention discloses human corneal limbus microenvironment cell exosome eye drops. The unique active ingredient in the eye drops is human corneal limbus microenvironment cell exosome. The invention aims to provide eye drops containing human corneal limbus microenvironment cell exosomes. The eye drops are suitable for repairing corneal injuries caused by various factors clinically and relieving eye diseases such as visual fatigue, xerophthalmia, corneal limbus stem cell deficiency and immune lesions caused by graft-versus-host diseases.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The invention provides an astigmatism axial position marker and a marking method, and relates to the technical field of medical instruments. The astigmatism axial position marker comprises a base, a control panel, a base and a display panel, the control panel is arranged in the front of the center of the upper end face of the base, the base is arranged in the rear of the center of the upper end face of the base, the display panel is arranged in the center of the front end face of the base, and a body is arranged on the upper end face of the base. A storage groove is formed in the upper center of the rear end face of the body. Facial feature points are extracted through image processing, the distance between the two eyes of a patient is calculated, the positions of the two flexible eyeshades are adjusted through the adjusting structure, the flexible eyeshades are made to be rapidly matched with the distance between the two eyes of the patient, and therefore it is ensured that the two monitoring marking structures can accurately detect and mark the cornea or a target area, and the detection accuracy is improved. Marking content can comprise specific reference marks (such as 0 degree, 180 degrees and 270 degrees) of corneal limbus or the axial position of an intraocular lens to be implanted and the like.

Capsid protein mutants capable of increasing the ability of AAVs to infect ocular tissue and the use thereof. A Cap mutant is obtained by replacing an amino acid sequence of an AAV capsid protein which is homologous to amino acids at positions D561 to R588 of a wild-type AAV2 capsid protein with one of the polypeptides as shown in SEQ ID NOs. 6-39. Some Cap mutants can infect a mouse retina from the RGC layer, cone and rod cells of INL and ONL, corneal limbus regions, trabecular meshwork, RPE and a small amount of RPE cells by administration via intravitreous injection, can solve the problem that it is difficult for the conventional serotype AAV to infect the cells in the posterior layer of the retina due to insufficient penetrability, and is expected to become a potential carrier for the treatment of glaucoma. Experimental data show that the abilities of some Cap mutants to infect and penetrate the retina of non-human primates are optimized, thereby providing a new tool for developing gene therapy preparations for ophthalmic diseases or constructing animal models related to ophthalmic diseases.

The invention relates to application of regulatory T cells and mesenchymal stem cells in preparation of medicines or medical instruments for repairing corneal nerve injury. The regulatory T cells are prepared from peripheral blood mononuclear cells, the adipose-derived stem cells are prepared from adipose tissues, the sources are wide, the adipose-derived stem cells are easy to obtain, the biocompatibility is good, the quantity is large and the purity is high after in-vitro extraction and amplification, the regulatory T cells and the adipose-derived stem cells are combined for use, corneal nerve repair can be promoted, and the treatment and repair effect is excellent. Further verification shows that corneal nerve repair is beneficial to corneal epithelium defect repair, corneal edema reduction and inhibition of generation of neovascularization and lymphatic vessels, and especially has a good application prospect in the aspect of corneal epithelium damage repair involving corneal limbus.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The invention relates to a construction method of tissue engineering corneal epithelial cells. The construction method comprises the following steps: obtaining corneal limbus tissue blocks; digesting the tissue block, and separating to obtain epithelial cells of the tissue block; the method comprises the following steps: attaching epithelial cells to ultrathin amniotic membrane-umbilical cord derived mesenchymal stem cells, culturing in an SHEM culture medium, changing the SHEM culture medium once every two days, culturing for a period of time, and culturing the epithelial cells for a period of time by using a gas-liquid interface culture method to promote the multi-layer growth of the epithelial cells, thereby obtaining the tissue engineering corneal epithelial cells. Through a controllable preparation scheme, the amniotic membrane carrier with a certain thickness is obtained so as to increase the transparency of the amniotic membrane carrier, meanwhile, the matrix surface of the amniotic membrane carrier is inoculated with MSC, the healthy corneal limbus matrix microenvironment of a human body is simulated, damaged corneal limbus tissue / corneal epithelium is better repaired or regenerated, and the ocular surface microenvironment is improved. The produced ultrathin amnion combined mesenchymal stem cell carrier can obviously improve the effect of a tissue engineering corneal epithelium transplantation operation.

The utility model discloses a novel vitreous cavity injector capable of being positioned, which comprises a guiding and positioning mechanism and an injector, the guiding and positioning mechanism comprises an outer cylinder and a positioning ring, the positioning ring is arranged at the front end of the outer cylinder, the positioning ring and the outer cylinder are of an integrated structure which is concentrically arranged, and a concentric annular silica gel gasket is arranged on the front end face of the positioning ring. The silica gel gasket is positioned on the flat part of the ciliary body behind the cornea sclera edge of the eyeball of the patient; and a metal needle catheter is arranged in the center of the front end surface of the outer cylinder. The silica gel gasket of the positioning ring at the front end of the outer cylinder is tightly attached to the cornea sclera edge and makes flexible contact with the eyeball, and the needle head can be directly positioned on the flat portion of the ciliary body of a patient; after the needle head is inserted into the eyeball of a patient, the positioning ring at the front end of the outer cylinder provides certain supporting force, so that unnecessary damage to the eyeball of the patient caused by shaking of the hand, slippage of the needle head and the like during medicine injection is prevented; the positioning ring is suitable for the outer diameter radians of various eyeballs, and can accurately position a 4 mm injection point behind the corneal limbus.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

Disclosed herein is a method of alleviating symptoms of ocular surface discomfort, the method comprising: topically applying a cold slurry adjacent to a corneal limbus of an eye of a patient, wherein the cold slurry comprises water and a freezing point depressant, wherein the topical application of the cold slurry is configured to cause a degree of numbing of a cornea of the eye for a period of time, and wherein an ocular sensation of the eye is restored following the period of time.

The invention discloses an intraoperative extracorporeal Schlemm tube positioning method and application of the intraoperative extracorporeal Schlemm tube positioning method in a glaucoma surgery, and the intraoperative extracorporeal Schlemm tube positioning method comprises the steps that a conjunctival flap with a dome as a base is manufactured, a full-thickness bulbar conjunctiva is cut off along the corneal limbus radian through a conjunctival shear, and the cut-off length is 8 + / -2 mm; performing blunt separation by clinging to the sclera surface to form a rectangular sclera area; the method comprises the following steps: manufacturing a superficial sclera flap taking limbus corneae as a substrate, forwards sectioning a sclera to a transparent cornea area, placing a mitomycin cotton piece under the sclera flap, staying for 3 minutes, and flushing; a light ring and a shadow ring are observed to be formed on the whole corneal limbus by means of irradiation of the positioning optical fiber device, and lineation marking is carried out; carrying out 1mm * 2mm deep sclera flap sectioning in the direction from 1mm behind the marking line to the limbus corneae until the limbus corneae is transparent; in the sectioning process, 0.3 mm-0. 5 mm in front of the marking line is the rear edge of the outer path Schlemm tube, the outer wall of the outer path Schlemm tube can be opened by conducting conventional fine sectioning on the outer path Schlemm tube at the position, positioning is accurate, and the surgical success rate can be improved.

The invention provides a biological amnion cornea surface transplantation device which comprises a fixing body which is a circular ring and is matched with the corneal limbus of a human body. The fixing support piece at least comprises a fixing loop, and the fixing loop is a metal wire made of shape memory metal; one end of the fixing loop is fixedly connected with the fixing main body, and the other end of the fixing loop is far away from the diameter direction of the fixing main body passing through the connection point of the fixing loop and the fixing main body; and at least two fixing support pieces are arranged on the periphery of the fixing main body. Deformation and recovery of the memory metal and structural characteristics of human eyeballs are ingeniously utilized, the fixing loops are clamped to penetrate through the amniotic membrane and the corneal limbus conjunctiva and then are loosened, the memory metal recovers deformation in the gap between the conjunctiva and the sclera, the at least two fixing loops form a clamping jaw in the gap between the conjunctiva and the sclera along the corneal limbus radian, and the clamping jaw is clamped by the memory metal. Therefore, the amniotic membrane is pressed on the surface of the cornea by the fixing main body. The device is simple in structure, low in cost and easy to operate, trauma in an operation is small, and postoperative recovery is fast.

The utility model provides a biological amnion cornea surface transplantation device which comprises a fixing body which is a circular ring and is matched with the corneal limbus of a human body. The fixing support piece at least comprises a fixing loop, and the fixing loop is a metal wire made of shape memory metal; one end of the fixing loop is fixedly connected with the fixing main body, and the other end of the fixing loop is far away from the diameter direction of the fixing main body passing through the connection point of the fixing loop and the fixing main body; and at least two fixing support pieces are arranged on the periphery of the fixing main body. Deformation and recovery of the memory metal and structural characteristics of human eyeballs are ingeniously utilized, the fixing loops are clamped to penetrate through the amniotic membrane and the corneal limbus conjunctiva and then are loosened, the memory metal recovers deformation in the gap between the conjunctiva and the sclera, the at least two fixing loops form a clamping jaw in the gap between the conjunctiva and the sclera along the corneal limbus radian, and the clamping jaw is clamped by the memory metal. Therefore, the amniotic membrane is pressed on the surface of the cornea by the fixing main body. The device is simple in structure, low in cost and easy to operate, trauma in an operation is small, and postoperative recovery is fast.

An incisional instrument and method of use for creating accurate, reproducible surgical incisions. An exemplary embodiment includes an incisional instrument configured for attachment to a patient's eye and for use performing arcuate limbal relaxing incisions (LRIs). The incisional instrument is made up of two coaxial, interconnecting pieces: a docking piece and a cutting piece. The docking piece includes a suction mechanism and is configured for being secured to a patient's eye just outside the corneal limbus. The cutting piece is configured to fit flush within the docking piece and includes cutting blades and one or more handles for rotating the cutting piece relative to the docking piece. When assembled, the cutting blades extend into a patient's eye a desired LRI depth. An embodiment further includes an arcuate guide template with stoppers for providing lateral, arcuate stops for precise cuts in the desired LRI locations. An LRI method includes the steps of utilizing the incisional instrument in an ophthalmologic LRI procedure.

The puncture needle comprises a puncture guide needle core and a flexible sleeve, the puncture guide needle core is sleeved with the flexible sleeve in a sliding mode, one end of the puncture guide needle core is arranged to be an inclined end with a certain angle relative to a body, and the other end of the puncture guide needle core is arranged to be an inclined end with a certain angle relative to the body. And the end part of one end, far away from the inclined end, of the flexible sleeve is blunt. The puncture guide needle core and the flexible sleeve enter from one end of the corneal limbus and then penetrate out of the corneal limbus on the opposite side, the puncture needle is fixed to the anterior chamber, the effect of preventing the crystalline lens from being disengaged is achieved, the risk that the crystalline lens is disengaged or even eye content is disengaged in the operation is reduced, meanwhile, after the suture stage in the operation is completed, the puncture guide needle core is firstly pulled out of the flexible sleeve, and the puncture guide needle core is pulled out of the flexible sleeve. The implant and the crystalline lens can be prevented from being damaged, the safety in use is improved, the insertion depth of the puncture guide needle core relative to the flexible sleeve can be limited through the design of the inclined end at one end of the puncture guide needle core, meanwhile, clamping of a needle holder is facilitated, and the stability and convenience in use are improved.

The utility model provides a chamber corner separator. The chamber corner separator comprises a handle part; each neck part is arranged at the end part of the handle part, and the neck part is designed to be of a long-strip-shaped structure or a bent structure; each arc-shaped bent ring is arranged at one end, far away from the handle part, of the corresponding neck part and is vertically arranged relative to the neck part, and a frosted layer is arranged on the surface of at least one arc-shaped bent ring. According to the chamber corner separator, the arc-shaped bent ring can be better matched with the curvature of corneal limbus, so that the separation position is deeper, the separation is more thorough, the chamber corner separator is suitable for large-range separation towards the left side and the right side, the structure of the arc-shaped bent ring is more suitable for separating chamber corners which are slightly adhered, have bleeding or are high in iris injury risk, and the bleeding problem is avoided. Meanwhile, the frosted layer is arranged on the surface of at least one arc-shaped bent ring, friction force can be increased, acting force can be effectively applied to the iris while iris tissue is not damaged, and the chamber corner separator has higher safety and higher operability.

The invention discloses a femtosecond laser refraction operation cornea center positioning marking device in the technical field of cornea refraction positioning in the ophthalmology department. The device comprises a positioning module, an acquisition module, a processing module, a control module, a prompting module and a plurality of light sources. The positioning module is composed of an inner ring and an outer ring, the outer ring is used for being aligned with the corneal limbus, and the inner ring is used for being aligned with the corneal central visual area and provided with a dyeing marking mechanism. And the light sources are circumferentially distributed around the fixation lamp. The acquisition module acquires a two-dimensional cornea image. The processing module integrates the images into a three-dimensional visual area image. And the control module generates a reflective point guide curve based on a reflective point area in the three-dimensional visual area image, and judges whether the cornea central visual area is accurately positioned or not. And the prompt module sends prompt information to an operator. Based on the three-dimensional visual area image and the reflective point guide curve, whether positioning of the inner ring and the outer ring is accurate or not is accurately judged, and the accuracy of cornea center positioning is improved.

The invention discloses a method and a system for acquiring the rotation angle of an eyeball, which can acquire the rotation angle of the eyeball in dynamic movement of the eyeball, and belongs to the technical field of computer-aided medical treatment. The method for acquiring the rotation angle of the eyeball comprises the following steps: 1, preparing data; step 2, positioning corneal limbus and eye center in the eye picture; step 3, calculating eyeball rotation angles in different eye pictures; 4, the corneal meridian and the surgical incision position in the eye picture are obtained, S41, the corneal meridian and surgical incision rotation angle is obtained through the eyeball rotation angle, and therefore the real-time motion state of the corneal meridian and the surgical incision in the operation is obtained.

The invention provides application of vasoactive intestinal peptide VIP in preparation of a medicine for preventing and / or treating corneal epithelium lesion, and belongs to the technical field of biological medicine preparation. The invention discloses application of vasoactive intestinal peptide VIP in preparation of a medicine for preventing and / or treating corneal epithelium lesion. Researches show that diabetes can cause expression down-regulation of VIP and a receptor VIPR1 thereof in corneal limbus tissues, so that proliferation activity of corneal limbus stem cells of diabetic mice is reduced, and healing of corneal epithelium wounds is delayed. The exogenous supplement of VIP can effectively reverse the pathological change, significantly enhance the multiplication capacity of corneal limbal stem cells and accelerate the repair of corneal epithelium wounds; on the contrary, the VIPR1 receptor is knocked out, so that the proliferation activity of the corneal limbal stem cells is reduced, and the epithelium repair is delayed. Based on the results, the invention provides a new application of VIP in preparation of medicines for preventing and / or treating corneal epithelium lesions, and a new effective means is provided for repair treatment of the diseases.

A micro reverse intraocular scissors comprises a main arm, a scissor arm is hinged at the front end of the main arm, a movable arm is further provided on the main arm, the movable arm is hingedly matched with the scissor arm and the main arm respectively, a cutting edge is formed on the inner side of the main arm and the scissor arm, an opening end of the cutting edge is formed between the main arm and the scissor arm and their hinge shafts, and an opening end of a power arm is formed between the main arm and the movable arm and their hinge shafts, and the opening end of the cutting edge and the opening end of the power arm are arranged in the same direction. Through the transmission matching relationship between the main arm, the movable arm and the scissor arm, the function of operating the proximal tissue of the corneal limbus is realized without the need for additional incision damage and operation procedures, thereby reducing the risk of traumatic damage to eye tissue and postoperative infection.