The invention provides a corneal thickness measuring method based on subdivision of pulses, which belongs to an ultrasonic thickness measuring method. Measurement precision of the corneal thickness measuring method can achieve the micrometer level. Subdivision of pulse is realized by the aid of phase shift of a transmitted pulse, an ultrasonic signal which is reflected back is received, the frontwall and the rear wall of a cornea are judged, a single measurement result is calculated, a next transmitted pulse shifts by a subdivided phase of a pi phase, an ultrasonic signal which is reflected back is received, a current measurement result is obtained, and after total transmitted pulses shift by the pi phase, a last measurement result is obtained. Accurate distances from two sides of the cornea to an ultrasonic sensor are calculated according to the phases corresponding to the maximum values of all the subdivision results, and the final cornea thickness value is obtained by means of subtracting the two accurate distances. If subdivided steps are N, measurement precision is 1 / N of semi-wavelength. The corneal thickness measuring method has the advantages that the ultrasonic thicknessmeasuring method is realized by the aid of subdivision of pulses, the measurement precision can achieve the micrometer level. Resolution ratio of the method is high, center frequency of a probe does not need to be too high, and the corneal thickness measuring method is easy to be popularized.