Patents
Literature
144 results about "Decellularization" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Decellularization (also spelt decellularisation in British English) is the process used in biomedical engineering to isolate the extracellular matrix (ECM) of a tissue from its inhabiting cells, leaving an ECM scaffold of the original tissue, which can be used in artificial organ and tissue regeneration. Organ and tissue transplantation treat a variety of medical problems, ranging from end organ failure to cosmetic surgery. One of the greatest limitations to organ transplantation derives from organ rejection caused by antibodies of the transplant recipient reacting to donor antigens on cell surfaces within the donor organ. Because of unfavorable immune responses, transplant patients suffer a lifetime taking immunosuppressing medication. Stephen F. Badylak pioneered the process of decellularization at the McGowan Institute for Regenerative Medicine at the University of Pittsburgh. This process creates a natural biomaterial to act as a scaffold for cell growth, differentiation and tissue development. By recellularizing an ECM scaffold with a patient’s own cells, the adverse immune response is eliminated. Nowadays, commercially available ECM scaffolds are available for a wide variety of tissue engineering. Using peracetic acid to decellularize ECM scaffolds have been found to be false and only disinfects the tissue.
Lenticules for intrastromal corneal implantation
Decellularized and shaped corneal tissue lenticules from allograft and / or xenograft sources and methods of obtaining such lenticules are disclosed. The lenticules are particularly useful as intrastromal lenticular implants in keratoplasty procedures, in which a hinged flap is formed in a patient's cornea and folded back along its hinge to expose the stromal bed of the cornea. The shaped lenticule is then applied to the stromal bed and the flap returned to its original position yielding a new curvature for the cornea and resulting in a desired refractive correction. Fine-tuning of the new refractive power can be achieved by laser ablation either at the same time as implantation or at later time in the event of regression or tonus changes. Methods of decellularizing cornea tissue are disclosed to reduce potential immunogenic reactions on the part of the patient to the implanted lenticule. The lenticules can be further treated to remove immunogenic epitopes. In addition, the posterior and / or anterior surfaces of the lenticule can be treated to assist in intrastromal seating of the lenticule and / or to reduce the likelihood of dislodgement. The methods of forming lenticules can further include preserving at least a portion of the Bowman's membrane. The smoothness of the Bowman's membrane surface also makes it less likely that reopening the flap will dislodge the lenticule.
Owner:GEBAUER-KLOPOTEK PATENT VERW ALTUNGS-UG
Acellular dermal matrix particle and preparation method thereof
The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.
Owner:PULIYAN (NANJING) MEDICAL TECH CO LTD
Biofabricated vaginal extracellular matrix hydrogel
PCT designated stage expiredWO2025114893A1Aerosol deliveryOintment deliveryCell-Extracellular MatrixDecellularization
OF THE INVENTION Provided herein is a method of preparing an extracellular matrix (ECM) material, including incubating vaginal tissue in a protease, incubating the vaginal tissue in a solution including at least one detergent, incubating the vaginal tissue in a composition including at least one disinfecting agent, thereby producing a decellularized ECM material, lyophilizing the decellularized ECM material, thereby producing a lyophilized ECM material, comminuting the lyophilized ECM material, partially or completely solubilizing the comminuted, lyophilized ECM material with an acid protease to produce solubilized ECM, lyophilizing the solubilized ECM to produce lyophilized, solubilized ECM.
Owner:UNIV OF PITTSBURGH OF THE COMMONWEALTH SYST OF HIGHER EDUCATION
Combined bioprosthetic valve material anti-calcification method and bioprosthetic valve material
The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.
Owner:QINGDAO GUOKE XINJIAN TECHNOLOGY CO LTD
Extracellular matrix fiber material as well as preparation method and application thereof
The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.
Owner:BEIJING DATSING BIO TECH
Acellular fish skin matrix biological scaffold as well as preparation method and application thereof
The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.
Owner:QINGDAO UNIV
Construction method and application of cytokine sustained-release complex combined with stem cells
The invention relates to a therapeutic drug, in particular to a construction method and application of a cell factor sustained-release complex combined with stem cells, and the cell factor sustained-release complex can be used for preventing intrauterine adhesion and promoting endometrial repair. The invention aims to provide a construction method and application of a cell factor slow-release complex combined with stem cells, so that the cell factor slow-release complex is effectively applied to repair of intrauterine adhesion and endometrium. According to the technical scheme, the construction method of the cell factor slow-release complex combined with the stem cells comprises the following steps: (1) preparing platelet-rich plasma, activating and enriching platelet-rich plasma cell factors, and adsorbing the released cell factors through hydrogel; (2) preparing a vascular extracellular matrix by using a decellularization technology, wherein the vascular extracellular matrix is used for adsorbing platelet-rich plasma cytokines; (3) acquiring umbilical cord mesenchymal stem cells; constructing an umbilical cord mesenchymal stem cell / extracellular matrix slow-release complex; and (4) preparing the cell factor slow-release complex combined with the stem cells.
Owner:ZHEJIANG UNIV
Hydrogel bio-ink, preparation method thereof, 3D printing biodegradable airway stent prepared from hydrogel bio-ink and application of hydrogel bio-ink
The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.
Owner:THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
Multifunctional hydrogel patch as well as preparation method and application thereof
ActiveCN121818989APharmaceutical delivery mechanismAbsorbent padsCell-Extracellular MatrixFreeze-drying
The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Porcine collagen chemical decellularization method based on supercritical fluid technology
The invention relates to the technical field of collagen, in particular to a pig collagen chemical decellularization method based on a supercritical fluid technology, which comprises the following steps: taking fresh pigskin collagen, cleaning, cutting, and carrying out physical pretreatment; putting the pretreated collagen into a supercritical reaction kettle, introducing supercritical fluid, controlling the pressure to be 30-42 MPa and the temperature to be 40-50 DEG C, and maintaining for 10-20 minutes to establish a supercritical environment; injecting a mixed reagent containing ethanol, protease and a chelating agent into the supercritical reaction kettle, and stirring for 1.0-2.0 hours; after the treatment is finished, reducing the pressure of the reaction kettle in a stepped manner at the rate of 0.5-1MPa / min, introducing supercritical fluid or a mixed fluid of the supercritical fluid and ethanol for replacement during the period, and finally reducing the pressure to normal pressure; and taking out the collagen, carrying out enzymolysis treatment, and washing with a sterile solution to obtain the collagen. And the DNA residual quantity is greatly reduced through supercritical multiphase fluid cooperation and a nano-enzyme directional delivery technology.
Owner:HANBANG MEDICAL SCI & TECH HARBIN CITY
Implantable acellular matrix material and preparation method thereof
The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.
Owner:深圳市迈捷生命科学有限公司
Valve preparation method
The invention discloses a valve preparation method. The method comprises the following steps: performing decellularization treatment on a bovine pericardium material to be treated; enhancing the porosity of the decellularized bovine pericardium material through a physical and / or chemical method so as to promote the regeneration of native valve leaflet cells and tissues, and obtaining a composite material; and cutting the leaflet of the composite material to obtain the valve. The bovine pericardial valve which is high in durability, free of anticoagulation, excellent in biocompatibility, excellent in calcification resistance and low in immunogenicity can be prepared, and therefore the purpose of treating heart valve diseases is achieved.
Owner:KOKA NANTONG LIFESCIENCES CO LTD
Establishment of a human decellularized ovary bank for regenerative medicine and culture of immature follicles of cryopreserved ovaries
PCT designated stageWO2026062625A2Cell culture supports/coatingCell culture active agentsDecellularizationFertility preservation
The present invention discloses a comprehensive methodology for engineering a functional artificial ovary through precision tissue engineering approaches, specifically designed to overcome the limitations of current fertility preservation techniques. The present invention specifically targets the fabrication of a biomimetic artificial ovary using decellularization-recellularization technology
Owner:UNIV UTE
BME-SIS biological membrane as well as preparation method and application thereof
The invention belongs to the technical field of medicines, and particularly relates to a BME-SIS biological membrane as well as a preparation method and application thereof. The BME-SIS biological membrane is provided through a screening preparation process based on an SIS material and through composite culture and decellularization treatment of the SIS material and BMSCs. The BME-SIS biological membrane has a good decellularization effect, and avoids the problem of insecurity caused by xenograft / allograft; compared with an SIS material, the BME-SIS biological membrane has the effects of remarkably promoting cell adhesion, proliferation and differentiation, is applied to a rat rotator cuff tearing model, is proved to have the effect of remarkably promoting rotator cuff tearing healing in multiple aspects of molecular biology, histology, motility, mechanics, imaging and the like, is beneficial to reducing the retearing risk after the rotator cuff operation, and has a good application prospect. And the treatment effect is stable. The BME-SIS biological membrane disclosed by the invention can be used for bioremediation patches, and has a good prospect in the aspect of rotator cuff tear healing.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
Preparation method of tendon tissue scaffold based on decellularization technology
The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.
Owner:HEBEI UNIV OF ENG
Decellularized artificial lymph drainage tube as well as preparation method and application thereof
The invention discloses a decellularized artificial lymph drainage tube and a preparation method and application thereof, and the preparation method of the decellularized artificial lymph drainage tube comprises the following steps: taking an animal blood vessel as a raw material, and performing decellularization treatment, cleaning treatment, enhancement treatment, hole forming treatment and drying treatment on the raw material. The decellularized artificial lymph drainage tube prepared by the preparation method can solve the problems of long consumed time and difficult operation of the existing lymphatic vessel-vein anastomosis operation, can drain the lymph at the swelling part of a patient to the vein system in time after implantation, and can quickly relieve the swelling symptom. The decellularized artificial lymph drainage tube supports ingrowth, migration and proliferation of autologous cells and is good in biocompatibility, the degradation time can be adjusted to be matched with the reconstruction time of an autologous lymphatic reflux system, and long-term patency is guaranteed.
Owner:SHENZHEN QIKANG MEDICAL DEVICES
Preparation method and application of adjustable porous fat foam
The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.
Owner:THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
Decellularization device for tendon repair material
ActiveCN223906828UBioreactor/fermenter combinationsBiological substance pretreatmentsRepair materialStructural engineering
The utility model discloses a tendon repair material decellularization device which comprises a fixed top plate, the lower surface of the fixed top plate is fixedly connected with a supporting rod, the lower surface of the supporting rod is fixedly connected with a bottom plate, the upper surface of the bottom plate is fixedly connected with a protection box body, a rotating round block is arranged in the protection box body, and the rotating round block is fixedly connected with the bottom plate. A circular block groove is formed in the upper surface of the rotating circular block, an interaction container is arranged on the surface of the circular block groove, a limiting circular plate is arranged over the rotating circular block, and an electric push rod is fixedly connected to the lower surface of the fixed top plate. Through the structure, the limiting circular plate, the rotating circular block and the interaction container are arranged, the limiting circular plate can be moved to extrude and limit the interaction container on the rotating circular block, and the rotating circular block is rotated to drive the interaction container to quickly rotate, so that tendon decellularization in the interaction container is in full contact with a decellularization solution, and the decellularization efficiency is effectively improved.
Owner:BEIJING KEJIAN BIOTECHNOLOGY CO LTD
Metformin-induced stem cell-derived extracellular matrix material, and preparation method and application thereof
The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.
Owner:SUZHOU UNIV
Nerve graft systems, devices, and methods
Systems, devices, and methods for treating a nerve injury in a patient are provided. A system for treating a patient includes at least one of a nerve graft-conduit or nerve cap-graft comprise a nerve segment derived from a tissue source. Methods for producing a medical device comprising a nerve segment, for obtaining an ideal degree of decellularization of a nerve segment, and for treating a patient with a medical device comprising a nerve segment are provided.
Owner:RENERVA LLC
Nerve conduit from decellularized plant that can be used with or without any drug release potential
PCT designated stageWO2026063889A1Tissue regenerationLiposomal deliveryPharmacy medicinePharmaceutical drug
The present invention relates to a nerve conduit obtained by plant decellularization, which does not require 3D printing, is multi-channel, low-cost and capable of drug release. This nerve conduit is functionalized with GelMA and has properties that promote nerve healing.
Owner:ISTANBUL UNIVSI CERRAHPASA REKTORLUGU
System and process for decellularizing blood vessels
A system and method for processing a tubular tissue sample defining a lumen and an exterior surface. The system includes a container having a one or more walls defining a hollow interior chamber for receiving the tubular tissue sample. A first fluid inlet passage is associated with the container and configured to direct fluid through the lumen of the tissue sample. A second fluid inlet passage is also associated with the container and configured to direct fluid over the exterior surface of the tissue sample.
Owner:LIFENET HEALTH
Modified acellular matrix
The invention relates to the technical field of medical biological materials, in particular to a modified acellular matrix. According to the method, microcrystal drying, immobilization and swelling treatment are combined in sequence in the decellularization treatment process, so that the phenomenon of low porosity after crosslinking can be effectively reduced, meanwhile, the mechanical loss in the decellularization process is reduced, and the high mechanical property is considered while the high porosity is obtained. According to the modified acellular matrix, the defect that the mechanical property of an acellular matrix material with the unit thickness is insufficient is remarkably overcome, and the modified acellular matrix has high porosity and high mechanical property at the same time. The acellular matrix is thin and tough, the comfort degree is high after implantation, the tensile strength is improved to more than three times that of an original material, and the acellular matrix has extremely high application value in the medical field with requirements for mechanical property, porosity, thickness and comfort degree.
Owner:YANTAI DESHENG MARINE BIOTECHNOLOGY CO LTD
In-vivo rapid recellularization tissue engineered blood vessel and preparation method thereof
PendingUS20250170303A1Pharmaceutical delivery mechanismTissue regenerationPerfusion CultureCellular component
An in-vivo rapid recellularization tissue engineered blood vessel and a preparation method thereof are provided. The preparation method includes: dissolving polyglycolic acid and polyethylene glycol in an organic solvent to prepare a spinning solution, and performing electrostatic spinning according to a certain proportion to prepare a tubular polymer composite support of a special tube wall structure, the fiber on the outer side of the tube wall support containing a higher proportion of polyethylene glycol; planting seed cells on the tubular polymer support of the special tube wall structure, and under certain culture conditions, simulating body arteriopalmus flow conditions and performing perfusion culture for 3 weeks to 3 months; and removing cell components from the tube wall of the obtained engineered blood vessel by using a decellularization method to give a tissue engineered blood vessel composed of an extracellular matrix of a bionic structure.
Owner:HUMATRIX MEDICAL TECH (SUZHOU) CO LTD
Process for the preparation of endothelialized matrix artificial blood vessels
The application provides a preparation method of an endothelium matrix vascular prosthesis. The method comprises the following steps: injecting a cell suspension into the inner wall of the vascular prosthesis to adhere, then supplying a culture medium under a perfusion circulation system to simulate an in-vivo vascular environment, so that an endothelium tissue is formed on the inner wall of the vascular prosthesis, and finally, the endothelium tissue vascular prosthesis is subjected to a decellularization treatment to obtain the endothelium matrix vascular prosthesis. The method can provide elasticity and mechanical properties for the vascular prosthesis by using a cell internal growth technology, and meanwhile, the adhesion and growth of cells are induced by using the topological structure of the vascular prosthesis. The method improves the biocompatibility of the vascular prosthesis while performing the endothelium matrix treatment on the vascular prosthesis, and finally, a biological vascular prosthesis with high elasticity, high mechanical strength and high biocompatibility is obtained.
Owner:WUHAN TEXTILE UNIV +1
Decellularization reactor
PendingJP2025539914ABioreactor/fermenter combinationsBiological substance pretreatmentsMedicineNuclear engineering
The decellularization reactor according to the present invention includes a mesh chamber for accommodating tissue of a xenogeneic organ to be decellularized, at least one reaction vessel for storing a decellularization solution, and a first reactor for circulating the decellularization solution between the inside and outside of the reaction vessel and analyzing various physical properties of the circulating decellularization solution in situ.
Owner:CLEANSOLUTION CO LTD +2
A thermosensitive hydrogel of porcine testicular acellular extracellular matrix and its preparation method
ActiveCN118161667BCell dissociation methodsPharmaceutical delivery mechanismCell-Extracellular MatrixTesticular Interstitial Cells
The present invention discloses a thermosensitive hydrogel of porcine testicular acellular extracellular matrix and a preparation method thereof, including: using freeze-thaw cycles to disrupt cell membranes, and performing decellularization treatment with a mild detergent TritonX-100. While effectively removing testicular cells, the biological composition and structural scaffold of the testis are highly retained. This hydrogel can rapidly solidify and form after being injected into the testis, adapt to the complex environment of the testis, recruit testicular interstitial stem cells at the same time, regulate their differentiation into testicular interstitial cells, and then secrete testosterone. In addition, the hydrogel can also degrade under the action of in vivo enzymes, release cytokines, and reduce the inflammatory reaction of testicular interstitium. The hydrogel also has high biological safety and low immunogenicity.
Owner:SOUTH CHINA UNIV OF TECH
Preparation method of heterogeneous bone-microporous metal implant composite material
The invention discloses a preparation method of a heterogeneous bone-microporous metal implant composite material, and belongs to the technical field of biology. The method comprises the following steps: implanting a microporous metal implant into a cancellous bone of a heterogenous animal, carrying out osseointegration connection on the microporous metal implant and the cancellous bone of the heterogenous animal after 12 weeks to form an interface interpenetrating structure, taking out the microporous metal implant, retaining a peripheral bone with the thickness of 1-10mm, and then sequentially carrying out gradient decalcification and complete decellularization treatment to obtain the porous metal implant of the heterogenous animal. The heterogeneous bone-microporous metal implant composite material with high bone induction activity, strong osseointegration and low immunogenicity is obtained; according to the invention, a metal implant material is converted into a bioactive implant composite material, and a heterogeneous interface of the metal implant and a transplanted bone is converted into a homogeneous interface of a host bone or a transplanted bone and a transplanted bone, so that the clinical problem that the interface osseointegration of the transplanted bone and the metal implant is poor is solved.
Owner:JILIN UNIVERSITY
An integrated device for generating decellularized organ patches
ActiveCN119752596BBioreactor/fermenter combinationsBiological substance pretreatmentsControl cellDecellularization
The application belongs to the field of medical instruments, and discloses an integrated device for generating a decellularized organ patch, which is designed as a decellularization chip, which comprises a decellularization processing module and an oscillator, the decellularization processing module is arranged above the oscillator and is fixedly connected with the oscillator; the decellularization processing module comprises: a middle layer, which comprises an open cavity arranged in the central region of the middle layer and a cell filter screen arranged on the open cavity; a bottom plate bonded to the lower layer of the middle layer, which is provided with a concave flow channel on the side facing the middle layer and is located in the orthographic projection region of the cell filter screen on the bottom plate; an upper cover bonded to the upper layer of the middle layer, which is provided with a liquid inlet in communication with the initial end of the concave flow channel and a liquid outlet in communication with the terminal end of the concave flow channel; and an integrated device formed by integrating a control unit, the decellularization chip and a fluid module. Based on the device, the decellularization can be controlled and adjusted to be completed in a stable and accurate environment.
Owner:HUAZHONG UNIV OF SCI & TECH
Preparation method of fat acellular extracellular matrix hydrogel
The invention belongs to the technical field of biological tissue engineering, and discloses a fat acellular extracellular matrix hydrogel preparation method, which comprises: (1) cutting cleaned fresh fat into pieces, and repeatedly freezing and thawing with a hypertonic salt solution; (2) homogenizing the frozen and thawed fat at low temperature, and collecting and precipitating; (3) re-suspending the precipitate by using PBS, oscillating in a shaking table, centrifuging at room temperature, and collecting the precipitate again; (4) carrying out degreasing and decellularization treatment on the collected precipitate by using a composite eluent; and (5) washing the precipitate with isopropanol for 24 hours, finally repeatedly washing the precipitate with distilled water, and collecting the precipitate, namely the fat acellular matrix. And (6) digesting the fat extracellular matrix with pepsin hydrochloride, and adjusting the pH value to be neutral with sodium hydroxide to obtain the fat extracellular matrix hydrogel. According to the preparation method of the fat extracellular matrix hydrogel, biological enzyme and high-speed centrifugation are not needed in the decellularization stage, the technology is simple, time consumption is short, the yield is high, the fat extracellular matrix prepared through the method is easy to digest, and the prepared hydrogel solution is uniform, good in injectability and capable of being used for production of a filling material for injection.
Owner:CHIMEDICAL UNIVERSITY