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70 results about "Decellularization" patented technology
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Decellularization (also spelt decellularisation in British English) is the process used in biomedical engineering to isolate the extracellular matrix (ECM) of a tissue from its inhabiting cells, leaving an ECM scaffold of the original tissue, which can be used in artificial organ and tissue regeneration. Organ and tissue transplantation treat a variety of medical problems, ranging from end organ failure to cosmetic surgery. One of the greatest limitations to organ transplantation derives from organ rejection caused by antibodies of the transplant recipient reacting to donor antigens on cell surfaces within the donor organ. Because of unfavorable immune responses, transplant patients suffer a lifetime taking immunosuppressing medication. Stephen F. Badylak pioneered the process of decellularization at the McGowan Institute for Regenerative Medicine at the University of Pittsburgh. This process creates a natural biomaterial to act as a scaffold for cell growth, differentiation and tissue development. By recellularizing an ECM scaffold with a patient’s own cells, the adverse immune response is eliminated. Nowadays, commercially available ECM scaffolds are available for a wide variety of tissue engineering. Using peracetic acid to decellularize ECM scaffolds have been found to be false and only disinfects the tissue.
Extracellular matrix fiber material as well as preparation method and application thereof
The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.
Owner:BEIJING DATSING BIO TECH
Multifunctional hydrogel patch as well as preparation method and application thereof
ActiveCN121818989APharmaceutical delivery mechanismAbsorbent padsCell-Extracellular MatrixFreeze-drying
The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Valve preparation method
The invention discloses a valve preparation method. The method comprises the following steps: performing decellularization treatment on a bovine pericardium material to be treated; enhancing the porosity of the decellularized bovine pericardium material through a physical and / or chemical method so as to promote the regeneration of native valve leaflet cells and tissues, and obtaining a composite material; and cutting the leaflet of the composite material to obtain the valve. The bovine pericardial valve which is high in durability, free of anticoagulation, excellent in biocompatibility, excellent in calcification resistance and low in immunogenicity can be prepared, and therefore the purpose of treating heart valve diseases is achieved.
Owner:KOKA NANTONG LIFESCIENCES CO LTD
Establishment of a human decellularized ovary bank for regenerative medicine and culture of immature follicles of cryopreserved ovaries
PCT designated stageWO2026062625A2Cell culture supports/coatingCell culture active agentsDecellularizationFertility preservation
The present invention discloses a comprehensive methodology for engineering a functional artificial ovary through precision tissue engineering approaches, specifically designed to overcome the limitations of current fertility preservation techniques. The present invention specifically targets the fabrication of a biomimetic artificial ovary using decellularization-recellularization technology
Owner:UNIV UTE
Preparation method of tendon tissue scaffold based on decellularization technology
The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.
Owner:HEBEI UNIV OF ENG
Preparation method and application of adjustable porous fat foam
The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.
Owner:THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
Decellularization device for tendon repair material
ActiveCN223906828UBioreactor/fermenter combinationsBiological substance pretreatmentsRepair materialStructural engineering
The utility model discloses a tendon repair material decellularization device which comprises a fixed top plate, the lower surface of the fixed top plate is fixedly connected with a supporting rod, the lower surface of the supporting rod is fixedly connected with a bottom plate, the upper surface of the bottom plate is fixedly connected with a protection box body, a rotating round block is arranged in the protection box body, and the rotating round block is fixedly connected with the bottom plate. A circular block groove is formed in the upper surface of the rotating circular block, an interaction container is arranged on the surface of the circular block groove, a limiting circular plate is arranged over the rotating circular block, and an electric push rod is fixedly connected to the lower surface of the fixed top plate. Through the structure, the limiting circular plate, the rotating circular block and the interaction container are arranged, the limiting circular plate can be moved to extrude and limit the interaction container on the rotating circular block, and the rotating circular block is rotated to drive the interaction container to quickly rotate, so that tendon decellularization in the interaction container is in full contact with a decellularization solution, and the decellularization efficiency is effectively improved.
Owner:BEIJING KEJIAN BIOTECHNOLOGY CO LTD
Metformin-induced stem cell-derived extracellular matrix material, and preparation method and application thereof
The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.
Owner:SUZHOU UNIV
Nerve conduit from decellularized plant that can be used with or without any drug release potential
PCT designated stageWO2026063889A1Tissue regenerationLiposomal deliveryPharmacy medicinePharmaceutical drug
The present invention relates to a nerve conduit obtained by plant decellularization, which does not require 3D printing, is multi-channel, low-cost and capable of drug release. This nerve conduit is functionalized with GelMA and has properties that promote nerve healing.
Owner:ISTANBUL UNIVSI CERRAHPASA REKTORLUGU
System and process for decellularizing blood vessels
A system and method for processing a tubular tissue sample defining a lumen and an exterior surface. The system includes a container having a one or more walls defining a hollow interior chamber for receiving the tubular tissue sample. A first fluid inlet passage is associated with the container and configured to direct fluid through the lumen of the tissue sample. A second fluid inlet passage is also associated with the container and configured to direct fluid over the exterior surface of the tissue sample.
Owner:LIFENET HEALTH
Process for the preparation of endothelialized matrix artificial blood vessels
The application provides a preparation method of an endothelium matrix vascular prosthesis. The method comprises the following steps: injecting a cell suspension into the inner wall of the vascular prosthesis to adhere, then supplying a culture medium under a perfusion circulation system to simulate an in-vivo vascular environment, so that an endothelium tissue is formed on the inner wall of the vascular prosthesis, and finally, the endothelium tissue vascular prosthesis is subjected to a decellularization treatment to obtain the endothelium matrix vascular prosthesis. The method can provide elasticity and mechanical properties for the vascular prosthesis by using a cell internal growth technology, and meanwhile, the adhesion and growth of cells are induced by using the topological structure of the vascular prosthesis. The method improves the biocompatibility of the vascular prosthesis while performing the endothelium matrix treatment on the vascular prosthesis, and finally, a biological vascular prosthesis with high elasticity, high mechanical strength and high biocompatibility is obtained.
Owner:WUHAN TEXTILE UNIV +1
An integrated device for generating decellularized organ patches
ActiveCN119752596BBioreactor/fermenter combinationsBiological substance pretreatmentsControl cellDecellularization
The application belongs to the field of medical instruments, and discloses an integrated device for generating a decellularized organ patch, which is designed as a decellularization chip, which comprises a decellularization processing module and an oscillator, the decellularization processing module is arranged above the oscillator and is fixedly connected with the oscillator; the decellularization processing module comprises: a middle layer, which comprises an open cavity arranged in the central region of the middle layer and a cell filter screen arranged on the open cavity; a bottom plate bonded to the lower layer of the middle layer, which is provided with a concave flow channel on the side facing the middle layer and is located in the orthographic projection region of the cell filter screen on the bottom plate; an upper cover bonded to the upper layer of the middle layer, which is provided with a liquid inlet in communication with the initial end of the concave flow channel and a liquid outlet in communication with the terminal end of the concave flow channel; and an integrated device formed by integrating a control unit, the decellularization chip and a fluid module. Based on the device, the decellularization can be controlled and adjusted to be completed in a stable and accurate environment.
Owner:HUAZHONG UNIV OF SCI & TECH
Preparation method of fat acellular extracellular matrix hydrogel
The invention belongs to the technical field of biological tissue engineering, and discloses a fat acellular extracellular matrix hydrogel preparation method, which comprises: (1) cutting cleaned fresh fat into pieces, and repeatedly freezing and thawing with a hypertonic salt solution; (2) homogenizing the frozen and thawed fat at low temperature, and collecting and precipitating; (3) re-suspending the precipitate by using PBS, oscillating in a shaking table, centrifuging at room temperature, and collecting the precipitate again; (4) carrying out degreasing and decellularization treatment on the collected precipitate by using a composite eluent; and (5) washing the precipitate with isopropanol for 24 hours, finally repeatedly washing the precipitate with distilled water, and collecting the precipitate, namely the fat acellular matrix. And (6) digesting the fat extracellular matrix with pepsin hydrochloride, and adjusting the pH value to be neutral with sodium hydroxide to obtain the fat extracellular matrix hydrogel. According to the preparation method of the fat extracellular matrix hydrogel, biological enzyme and high-speed centrifugation are not needed in the decellularization stage, the technology is simple, time consumption is short, the yield is high, the fat extracellular matrix prepared through the method is easy to digest, and the prepared hydrogel solution is uniform, good in injectability and capable of being used for production of a filling material for injection.
Owner:CHIMEDICAL UNIVERSITY
Sealing of decellularized and recellularized artificial organ grafts
To provide a reinforced decellularized and recellularized artificial organ graft by providing a method for sealing the decellularized and recellularized artificial organ graft.SOLUTION: A method of reducing the porosity of an outer fibrous layer of a decellularized mammalian organ or tissue or portion thereof, the method comprising contacting an exterior surface of a mammalian organ or tissue or portion thereof comprising a vascular tree and cells with a composition comprising at least one agent in an amount to form a coating on the exterior surface, wherein: Wherein the coating is effective to increase the retention of fluid in the organ or tissue relative to fluid introduced into the corresponding organ or tissue or portion thereof prior to exposure to the composition, and decellularizing the coated mammalian organ or tissue or portion thereof by perfusion decellularization.SELECTED DRAWING: None
Owner:MIROMATRIX MEDICAL INC
Tissue matrix decellularized in multiple stages, and preparation method therefor
The present invention relates to a multi-stage decellularized mammal-derived tissue matrix, prepared by a method comprising: a preparation step for preparing tissue of a non-human mammal; a pretreatment step for pretreating the tissue; a first inactivation step for inactivating a virus included in the pretreated tissue using alcohol; a primary decellularization step for removing cells from the virus-inactivated tissue using an aqueous basic solution; a secondary decellularization step for removing cells by enzymatically treating the primary decellularized tissue; and a second inactivation step for inactivating a virus included in the decellularized tissue using an acid, and wherein the DNA content in the tissue subjected to the second inactivation step is 50 ng / mg or less, and the reduction rate (L) of the elastin content in the tissue subjected to the pretreatment step to the second inactivation step is 20% or less; and a method for preparing the same. The tissue matrix of the present invention has the advantage that immune responses or foreign body reactions are minimized since the DNA content is very low, and the wound healing and scar suppression effects are high since the elastin content is high.
Owner:T&R BIOFAB CO LTD
Functionalized decellularized porcine cornea, decellularized porcine cornea composite scaffold containing layered slow-release bioactive molecules and preparation method of decellularized porcine cornea composite scaffold
The invention discloses a functional decellularized porcine cornea, a decellularized porcine cornea composite scaffold containing layered slow-release bioactive molecules and a preparation method of the functional decellularized porcine cornea composite scaffold. The preparation method of the functionalized decellularized pig cornea comprises the following steps: (1) pretreating raw materials; (2) carrying out decellularization treatment; (3) carrying out functionalization treatment on the decellularized porcine cornea and the like. Researches show that the functionalized decellularized porcine cornea prepared by the method disclosed by the invention can well load bioactive molecules. In addition, the functionalized decellularized porcine cornea prepared by the method disclosed by the invention also has better mechanical properties. Furthermore, based on the functionalized decellularized porcine cornea, a layered sustained-release design and a 3D printing customization technology are adopted, the cornea scaffold with anti-inflammatory, regeneration promotion and neural restoration functions is constructed, breakthrough is achieved in the aspects of immune compatibility, mechanical properties and curative effect controllability, and an innovative solution is provided for cornea disease treatment.
Owner:GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
A porcine collagen chemical decellularization method based on supercritical fluid technology
The application relates to the technical field of collagen, in particular to a pig collagen chemical decellularization method based on supercritical fluid technology, which comprises the following steps: taking fresh pig skin collagen, performing physical pretreatment after washing and cutting; placing the pretreated collagen in a supercritical reaction kettle, introducing supercritical fluid, controlling the pressure to be 30-42 MPa and the temperature to be 40-50 DEG C, maintaining the supercritical environment for 10-20 minutes; injecting a mixed reagent containing ethanol, protease and chelating agent into the supercritical reaction kettle, stirring for 1.0-2.0 hours; after the treatment is completed, the pressure of the reaction kettle is reduced in steps at a rate of 0.5-1 MPa / min, and supercritical fluid or mixed fluid of supercritical fluid and ethanol is introduced during the replacement, and finally the pressure is reduced to normal pressure; the collagen is taken out, subjected to enzymatic hydrolysis treatment, washed with a sterile solution, and the pig collagen is obtained. Through the supercritical multiphase fluid cooperation and nano-enzyme directional delivery technology, the DNA residual amount is greatly reduced.
Owner:HANBANG MEDICAL SCI & TECH HARBIN CITY
Wound dressing based on silver-loaded polygonatum sibiricum polysaccharide composite electrostatic spinning decellularized fish skin and preparation method and application thereof
The application belongs to the technical field of biomedical materials, and particularly relates to a wound dressing based on silver-loaded polygonatum sibiricum polysaccharide composite electrostatic spinning decellularized fish skin as well as a preparation method and application thereof. The wound dressing has a sandwich structure, and an outer layer thereof is formed by polygonatum sibiricum polysaccharide modified by silver nanoparticles and combined with a coaxial electrostatic spinning technology. A middle layer thereof is a decellularized fish skin obtained by treating fish skin by using a decellularization technology. The wound dressing prepared by the application has good surface hydrophilicity, antibacterial performance and biocompatibility, is beneficial to adhesion with a wound tissue, can effectively inhibit bacterial breeding at a wound, can promote cell proliferation and migration, can significantly accelerate full-thickness skin regeneration, and has the ability of promoting wound healing.
Owner:TAIZHOU UNIV
Hybrid biomatrix and method for producing same
The invention relates to the field of biotechnology, medicine and cosmetology, and more particularly to a hybrid matrix for use as a biological graft in the preparation of cardiac prostheses. The technical result of the invention includes improving the surface biocompatibility and the stability and durability of a biological graft which provides an antimicrobial response, and also preventing calcification. A method for producing a hybrid biomatrix comprises the following steps: pre-purifying a base of a biomatrix for 0.75-6 hours in two stages, consisting in the decellularization of xenopericardium in a 2-5 wt% water-alcohol solution of Tween-80, saturated with CO2, at a temperature of 20-400°C and a pressure of 10-30 MPa, and the subsequent decellularization of the xenopericardium in a multi-component system containing a solution based on supercritical CO2 and a 1-1.5 vol% addition of a 2-5 wt% water-alcohol solution of Tween-80, at a temperature of 20-400°C and a pressure of 10-30 MPa; stabilizing the biomatrix base by treatment with a 0.1-0.5 wt% aqueous buffer solution of genipin having a pH of 6.0-7.4 for 3-4 hours; and applying to the pre-purified biomatrix base a coating consisting of an aqueous solution of chitosan nanoparticles with an antimicrobial doping additive in the form of silver or vancomycin nanoparticles in a carbonic acid solution, at a pressure of 20-30 MPa and a temperature of 23-270°C for 1-6 hours.
Owner:OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTYU MNT
A whole fish scale-based bone repair material and a preparation method thereof
The present application relates to the technical field of biomaterials and regenerative medicine, and particularly relates to a preparation method of a whole-fish-scale-based bone repair material, comprising the following steps: S1, fish scale fiber preparation: collected fish scales are washed and subjected to decellularization treatment, and then ground into fibrous form; S2, fish scale collagen adhesive preparation: collagen is extracted from the decellularized fish scales by using at least two different extraction methods, the obtained collagen products are mixed, freeze-dried, and then dissolved in deionized water to prepare collagen adhesives with different concentrations; S3, composite material preparation: the collagen adhesive prepared in step S2 is mixed with the fish scale fibers obtained in step S1, and then extruded into a shape; the whole-fish-scale-based bone tissue engineering material can be prepared by combining fish scale collagen and fish scale fibers, the material is not only safer and more reliable in composition, but also allows accurate regulation of the composition, thereby providing more innovative possibilities for the application of fish scale collagen adhesive.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA
Nanoparticle composition based on perinatal tissue as well as preparation method and application of nanoparticle composition
The invention provides a nanoparticle composition based on perinatal tissue as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The nanoparticle composition disclosed by the invention is obtained by decellularizing and purifying perinatal tissues. The nano-particle composition obtained by the invention contains rich cell factors and growth factors and more diverse miRNA (micro Ribonucleic Acid) components, and is applied to related joints. Clinical tests carried out in the sports and joint medicine department of the tenth people hospital in Shanghai and a rat model are passed, after the nano-particle composition is injected into the rat model, the pain and function of osteoarthritis can be effectively improved, and no adverse event is generated. The invention provides a novel safe and effective treatment way for promoting tissue repair and regeneration.
Owner:YIMITI (SHANGHAI) BIOTECHNOLOGY CO LTD
THPC cross-linked heart valve and preparation method thereof
The invention discloses a THPC cross-linked heart valve and a preparation method thereof. The preparation method of the THPC cross-linked heart valve comprises the following steps: performing decellularization treatment on biological tissues; soaking the biological tissue subjected to the decellularization treatment in a THPC cross-linking solution, and carrying out a cross-linking reaction; and after a preset time period, a reducing agent is adopted to terminate the cross-linking reaction and wash the biological tissue, and the THPC cross-linked heart valve is obtained. Through the mode, the preparation method of the THPC cross-linked heart valve, provided by the invention, can be used for preparing the THPC cross-linked heart valve under a mild reaction condition, so that the original characteristics of biological tissues can be reserved to the greatest extent, and various mechanical properties of the biological tissues can be improved; and the low toxicity of THPC ensures that the cross-linked material does not cause serious cytotoxic reaction and immunoreaction in the process of implanting into a human body or contacting with human tissues, so that the adhesion, proliferation and differentiation of cells on the surface of the material are facilitated, the repair and regeneration of tissues are promoted, and the safety and effectiveness of biomedical products are ensured.
Owner:HUBEI UNIV OF TECH +1
Biologic composition for promoting wound healing and a method of preparing the same
A biologic composition for promoting wound healing includes: a human mesenchymal stem cell (MSC)-derived extracellular matrix (ECM) biologic component, and a negatively charged polymer, combined with the biologic component to form the biologic composition. A method of preparing the biologic composition thereof includes: (i) isolating human mesenchymal stem cells (MSC) and subjecting the MSC to hypoxia-priming in a xeno- and / or serum-free, or chemically defined medium to stimulate the production of an MSC-derived biologic component with pro-angiogenic factors; (ii) aggregating and co-precipitating the MSC-derived biologic component with a negatively charged polymer into a pericellular space of the MSC to produce an aggregated biologic component; (iii) assembling the aggregated biologic component and the negatively charged polymer into an insoluble extracellular matrix (ECM) by the MSC; and (iv) decellularizing the insoluble ECM to obtain the biologic composition comprising MicroParticles of Solidified Secretome (MIPSOS).
Owner:CENTER FOR NEUROMUSCULOSKELETAL RESTORATIVE MEDICINE LIMITED
Multifunctional hydrogel patch and preparation method and application thereof
The application discloses a multifunctional hydrogel patch, a preparation method and application thereof, and belongs to the field of tissue engineering and regenerative medicine. The biological patch comprises a decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs). The dFCT is obtained by decellularization and freeze-drying treatment of tumor tissue, and retains abundant natural extracellular matrix components. The HNPs are extracted from hair and have excellent active oxygen scavenging capacity and photothermal conversion performance. The hydrogel layer is preferably methacrylated gelatin (GelMA). The patch shows significant cell activity promotion and antibacterial capacity in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch has the advantages of simple preparation process, natural component source, multiple functions such as antibacterial, antioxidant and tissue regeneration promotion, and is suitable for wound repair treatment.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Self-adaptive retractable posterior sclera biological patch
The invention discloses a self-adaptive retractable posterior sclera biological patch which comprises a posterior sclera biological patch body and a transmembrane nail. The biological patch is prepared from an animal-derived material through the procedures of decellularization, combing, cross-linking and forming. The transmembrane nails are of C-shaped structures made of shape memory alloy, and are unfolded and embedded into the sideband of the patch at the temperature of less than 35 DEG C to be distributed along the warp; the deformation temperatures of the transmembrane nails are respectively 35 DEG C, 37 DEG C, 39 DEG C and 41 DEG C; after the transmembrane nail is unfolded, the width of the openings at the two ends is 1.5-2 times of the original width. The transmembrane nail is implanted into the posterior pole part of the sclera through an operation to tightly hold a macular region, and under the action of body temperature or external heat after the operation, the transmembrane nail recovers to the original state and continuously acts on the posterior sclera, so that the posterior sclera is slowly shrunk, the ocular axis is shortened, and high myopia is corrected. The device has the characteristics that the structure is simple, the use is convenient, the acting force is lasting, the posterior sclera is slowly and gradually tightened, and the ocular axis is adaptively shortened.
Owner:NASKA BIOTECHNOLOGY (JIAXING) CO LTD
Decellularized amniotic membrane matrix hydrogel and method of preparation thereof
Provided are a decellularized amniotic membrane matrix hydrogel and its preparation method, which includes the following steps: pre-treatment of an amniotic membrane tissue, washing, decellularization, grinding, freeze-drying, digestion, and acid-base neutralization processes. The decellularized amniotic membrane matrix hydrogel provided in this disclosure retains components such as collagen, fibronectin, and glycoproteins from the amniotic membrane while removing viable cells, thereby reducing its immunogenicity at the source. The decellularized amniotic membrane matrix hydrogel may mimic the microenvironment in which cells exist in vivo, providing the necessary support and signals for cell adhesion and growth. It can be used to construct tissue engineering scaffolds, supporting directed cell growth and tissue regeneration. Furthermore, the decellularized amniotic membrane matrix hydrogel may also serve as a drug carrier for in vivo drug release and therapy, demonstrating significant potential for application in medical research and clinical settings.
Owner:TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
Spoke-shaped acellular cornea and preparation method and application thereof
The application discloses a spoke-shaped decellularized cornea and a preparation method and application thereof, and belongs to the technical field of biomaterials and tissue engineering. The application realizes an orderly process of the phase change of water molecules along the temperature gradient direction by establishing a gradient temperature field from the periphery to the center of a sheet-shaped biological corneal tissue, and forms spoke-shaped microchannels in the cornea. The spoke-shaped microchannels can accelerate the decellularization process of the central region of the cornea, avoid excessive decellularization of the peripheral region of the cornea to cause excessive loss of bioactive components of the extracellular matrix, form a biomimetic migration path between the cornea and the receptor, facilitate the migration and infiltration of the cells of the receptor tissue into the cornea, promote the biological healing of the transplanted cornea and the limbus of the receptor cornea, and improve the biological stability of the transplanted cornea.
Owner:SICHUAN UNIV
Biological tissue decellularization method and biological tissue
The invention provides a biological tissue decellularization method and a biological tissue, and relates to the technical field of biological materials. The biological tissue decellularization method provided by the invention comprises the following steps: soaking a disinfected target biological tissue in a degreasing solution to obtain a degreased target biological tissue, the degreasing solution comprising a detergent, and the degreasing solution further comprising at least one of a polyol reagent and an extracellular matrix component; and soaking the degreased target biological tissue with a pancreatin solution to obtain the decellularized target biological tissue.
Owner:SUZHOU KEYI MEDICAL TECHNOLOGY CO LTD
Enzymatic treatment of tissues intended for implantation
Methods of preparing a tissue for surgical use are described herein. The method may include providing a tissue and treating the tissue with a solution including at least one of serrapeptase or nattokinase. The use of the serrapeptase or nattokinase results in an improvement in the pliability and the porosity of the tissue and assists in the decellularization of the tissue.
Owner:ELUTIA INC
Flexible pericardium biofilm as well as preparation method and application thereof
The invention discloses a flexible pericardium biofilm, a preparation method thereof and application of the flexible pericardium biofilm in manufacturing of heart valves and tissue repair materials. The preparation method comprises the following steps: pretreating fresh pericardium, soaking the pretreated fresh pericardium in a decellularization solution, oscillating with a shaking table for a period of time, cleaning, soaking the cleaned pericardium in a cross-linking solution, standing for cross-linking for a period of time, and cleaning; the decellularization liquid is prepared from an ionic surfactant and a first non-ionic surfactant; the cross-linking liquid comprises a second nonionic surfactant and a cross-linking agent. The decellularization time is 2-10 hours, which is lower than that of other disclosed technologies, so that the risk caused by microorganisms is further reduced; according to the cross-linking process, under the participation of a second nonionic surfactant, cross-linking is carried out after the state of the pericardial collagen fibers is changed, the cross-linking probability of the crude fibers is reduced, and therefore the soft state after overall cross-linking is achieved.
Owner:NINGBO MICHI TECH CO LTD