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182 results about "Decellularization" patented technology
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Decellularization (also spelt decellularisation in British English) is the process used in biomedical engineering to isolate the extracellular matrix (ECM) of a tissue from its inhabiting cells, leaving an ECM scaffold of the original tissue, which can be used in artificial organ and tissue regeneration. Organ and tissue transplantation treat a variety of medical problems, ranging from end organ failure to cosmetic surgery. One of the greatest limitations to organ transplantation derives from organ rejection caused by antibodies of the transplant recipient reacting to donor antigens on cell surfaces within the donor organ. Because of unfavorable immune responses, transplant patients suffer a lifetime taking immunosuppressing medication. Stephen F. Badylak pioneered the process of decellularization at the McGowan Institute for Regenerative Medicine at the University of Pittsburgh. This process creates a natural biomaterial to act as a scaffold for cell growth, differentiation and tissue development. By recellularizing an ECM scaffold with a patient’s own cells, the adverse immune response is eliminated. Nowadays, commercially available ECM scaffolds are available for a wide variety of tissue engineering. Using peracetic acid to decellularize ECM scaffolds have been found to be false and only disinfects the tissue.
Lenticules for intrastromal corneal implantation
Decellularized and shaped corneal tissue lenticules from allograft and / or xenograft sources and methods of obtaining such lenticules are disclosed. The lenticules are particularly useful as intrastromal lenticular implants in keratoplasty procedures, in which a hinged flap is formed in a patient's cornea and folded back along its hinge to expose the stromal bed of the cornea. The shaped lenticule is then applied to the stromal bed and the flap returned to its original position yielding a new curvature for the cornea and resulting in a desired refractive correction. Fine-tuning of the new refractive power can be achieved by laser ablation either at the same time as implantation or at later time in the event of regression or tonus changes. Methods of decellularizing cornea tissue are disclosed to reduce potential immunogenic reactions on the part of the patient to the implanted lenticule. The lenticules can be further treated to remove immunogenic epitopes. In addition, the posterior and / or anterior surfaces of the lenticule can be treated to assist in intrastromal seating of the lenticule and / or to reduce the likelihood of dislodgement. The methods of forming lenticules can further include preserving at least a portion of the Bowman's membrane. The smoothness of the Bowman's membrane surface also makes it less likely that reopening the flap will dislodge the lenticule.
Owner:GEBAUER-KLOPOTEK PATENT VERW ALTUNGS-UG
Acellular dermal matrix particle and preparation method thereof
The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.
Owner:PULIYAN (NANJING) MEDICAL TECH CO LTD
Biofabricated vaginal extracellular matrix hydrogel
PCT designated stage expiredWO2025114893A1Aerosol deliveryOintment deliveryCell-Extracellular MatrixDecellularization
OF THE INVENTION Provided herein is a method of preparing an extracellular matrix (ECM) material, including incubating vaginal tissue in a protease, incubating the vaginal tissue in a solution including at least one detergent, incubating the vaginal tissue in a composition including at least one disinfecting agent, thereby producing a decellularized ECM material, lyophilizing the decellularized ECM material, thereby producing a lyophilized ECM material, comminuting the lyophilized ECM material, partially or completely solubilizing the comminuted, lyophilized ECM material with an acid protease to produce solubilized ECM, lyophilizing the solubilized ECM to produce lyophilized, solubilized ECM.
Owner:UNIV OF PITTSBURGH OF THE COMMONWEALTH SYST OF HIGHER EDUCATION
3D printed bone defect repair scaffold loaded with extracellular matrix of mesenchymal stem cells and preparation method thereof
A 3D printed bone defect repair scaffold loaded with extracellular matrix of mesenchymal stem cells is provided, its preparation method includes: dissolving gelatin, sodium alginate and 58S bioglass in water to obtain a solution, stirring the solution evenly to obtain 3D printing slurry, and performing 3D printing; chemically cross-linking the semi-finished scaffold with a calcium chloride solution, followed by soaking in a glutaraldehyde solution to chemically cross-link, and cleaning and freeze-drying to obtain the 3D printed scaffold; sterilizing the 3D printed scaffold; seeding rBMSC suspension on the sterilized 3D printed scaffold at a concentration of at least 106 cells / well, and culturing rBMSC by using a low glucose DMEM with 10% FBS for 2 weeks; and taking out the scaffold to perform a decellularization treatment, and freeze-drying the decellularized 3D printed scaffold to obtain the 3D printed bone defect repair scaffold loaded with extracellular matrix of mesenchymal stem cells.
Owner:HOSPITAL OF STOMATOLOGY GUANGZHOU MEDICAL UNIVERSITY (YANGCHENG HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY)
Combined bioprosthetic valve material anti-calcification method and bioprosthetic valve material
The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.
Owner:QINGDAO GUOKE XINJIAN TECHNOLOGY CO LTD
Extracellular matrix fiber material as well as preparation method and application thereof
The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.
Owner:BEIJING DATSING BIO TECH
A method for preparing acellular cartilage matrix material derived from pig rib cartilage
ActiveCN118995587BReservation of validityEfficient removalSkeletal/connective tissue cellsProsthesisCell-Extracellular MatrixActive agent
The present invention relates to the field of biomedical materials, and in particular to a method for preparing a decellularized cartilage matrix material derived from pig rib cartilage, comprising the following steps: (1) freezing and thawing the pig rib cartilage for 5 times; (2) soaking the pig rib cartilage frozen and thawed in step (1) in a sodium borohydride solution; (3) soaking the pig rib cartilage treated with sodium borohydride in step (2) in a sodium dodecyl sulfate solution; and (4) enzymolyzing the pig rib cartilage obtained in step (3) with a mixed solution of DNase and RNase. The present invention is suitable for decellularization of cartilage tissue with a thickness of 300 microns, and utilizes the spontaneous generation of hydrogen by the sodium borohydride solution to effectively expand the cartilage tissue, and then the surfactant SDS fully destroys the cell membrane, effectively removes the genetic material, and retains the effective components or extracellular matrix of the natural cartilage to the greatest extent.
Owner:THE THIRD AFFILIATED HOSPITAL OF SOUTHERN MEDICAL UNIV (ACAD OF ORTHOPEDICS GUANGDONG PROVINCE)
Acellular fish skin matrix biological scaffold as well as preparation method and application thereof
The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.
Owner:QINGDAO UNIV
Method for digesting extracellular matrix by immobilized enzyme and application
PendingCN119499455AFully contactedHigh biosecurityPharmaceutical delivery mechanismTissue regenerationEnzymatic digestionCell-Extracellular Matrix
The invention discloses a method for digesting an extracellular matrix by an immobilized enzyme and application, and relates to the technical field of biology. The method comprises the following steps: (1) performing decellularization treatment on tissues to obtain an extracellular matrix; (2) dispersing the surface functionalized microspheres in a buffer solution, adding a coupling agent, mixing, and collecting the activated microspheres; (3) dispersing the activated microspheres in a buffer solution containing pepsin, mixing, and collecting immobilized enzyme microspheres; and (4) crushing the extracellular matrix, dispersing the crushed extracellular matrix in a solution, adding the immobilized enzyme microspheres for digestion, and separating and removing the immobilized enzyme microspheres after digestion is completed. According to the method provided by the invention, the enzymolysis of the immobilized enzyme on the extracellular matrix is realized by adopting a proper microsphere particle size and a proper zeta potential. According to the method disclosed by the invention, the residual quantity of free enzyme is relatively small, so that the biological safety of the extracellular matrix material is improved.
Owner:BEIJING SIERGEN BIOTECHNOLOGY CO LTD
Acellular matrix and preparation method thereof
The invention relates to the technical field of medical biological materials, in particular to an acellular matrix and a preparation method thereof. The preparation method comprises the steps of decellularization, stabilization, crosslinking and cleaning. The cross-linking comprises circulating standing cross-linking in a cross-linking agent solution and ultrasonic equilibrium in an equilibrium buffer solution system, and each time of completion of standing cross-linking and ultrasonic equilibrium is a circulating process; the concentration of the cross-linking agent is increased for next cross-linking after each circulation. The invention provides the preparation method of the acellular matrix material with higher mechanical property and cell proliferation rate, the prepared acellular matrix material is thin and tough, the problems of poor mechanical property, poor comfort and low cell proliferation rate in the prior art are solved, and the acellular matrix material has higher value in the field of medical application and is suitable for popularization and application. The method is especially suitable for the field of sports medicine and has wide application prospects.
Owner:YANTAI DESHENG MARINE BIOTECHNOLOGY CO LTD
Construction method and application of cytokine sustained-release complex combined with stem cells
The invention relates to a therapeutic drug, in particular to a construction method and application of a cell factor sustained-release complex combined with stem cells, and the cell factor sustained-release complex can be used for preventing intrauterine adhesion and promoting endometrial repair. The invention aims to provide a construction method and application of a cell factor slow-release complex combined with stem cells, so that the cell factor slow-release complex is effectively applied to repair of intrauterine adhesion and endometrium. According to the technical scheme, the construction method of the cell factor slow-release complex combined with the stem cells comprises the following steps: (1) preparing platelet-rich plasma, activating and enriching platelet-rich plasma cell factors, and adsorbing the released cell factors through hydrogel; (2) preparing a vascular extracellular matrix by using a decellularization technology, wherein the vascular extracellular matrix is used for adsorbing platelet-rich plasma cytokines; (3) acquiring umbilical cord mesenchymal stem cells; constructing an umbilical cord mesenchymal stem cell / extracellular matrix slow-release complex; and (4) preparing the cell factor slow-release complex combined with the stem cells.
Owner:ZHEJIANG UNIV
Hydrogel bio-ink, preparation method thereof, 3D printing biodegradable airway stent prepared from hydrogel bio-ink and application of hydrogel bio-ink
The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.
Owner:THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
Multifunctional hydrogel patch as well as preparation method and application thereof
ActiveCN121818989APharmaceutical delivery mechanismAbsorbent padsCell-Extracellular MatrixFreeze-drying
The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.
Owner:THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Carrier cell sheet with anti-inflammatory and osteogenic activity and preparation method thereof
PendingCN119424755Agood biocompatibilitypromote proliferationSkeletal/connective tissue cellsCell culture supports/coatingTissue repairDecellularization
The invention discloses a cell sheet taking an acellular biological membrane as a carrier and having osteogenic activity as well as a preparation method and application of the cell sheet. The method comprises the following steps: firstly, carrying out decellularization treatment on a natural amnion, and then planting target cells on a biological membrane; and culturing the preliminarily constructed biological membrane to obtain the cell sheet taking the decellularized biological membrane as the carrier. The cell sheet prepared by the invention has excellent capability of promoting cell proliferation and osteogenic differentiation, so that tissue repair is promoted. The prepared cell sheet overcomes the defect that existing cell transplantation carriers generally lack activity, the preparation process is simple and controllable, and breakthrough of stem cell therapy in tissue engineering is facilitated.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Porcine collagen chemical decellularization method based on supercritical fluid technology
The invention relates to the technical field of collagen, in particular to a pig collagen chemical decellularization method based on a supercritical fluid technology, which comprises the following steps: taking fresh pigskin collagen, cleaning, cutting, and carrying out physical pretreatment; putting the pretreated collagen into a supercritical reaction kettle, introducing supercritical fluid, controlling the pressure to be 30-42 MPa and the temperature to be 40-50 DEG C, and maintaining for 10-20 minutes to establish a supercritical environment; injecting a mixed reagent containing ethanol, protease and a chelating agent into the supercritical reaction kettle, and stirring for 1.0-2.0 hours; after the treatment is finished, reducing the pressure of the reaction kettle in a stepped manner at the rate of 0.5-1MPa / min, introducing supercritical fluid or a mixed fluid of the supercritical fluid and ethanol for replacement during the period, and finally reducing the pressure to normal pressure; and taking out the collagen, carrying out enzymolysis treatment, and washing with a sterile solution to obtain the collagen. And the DNA residual quantity is greatly reduced through supercritical multiphase fluid cooperation and a nano-enzyme directional delivery technology.
Owner:HANBANG MEDICAL SCI & TECH HARBIN CITY
Fat acellular matrix hydrogel material and preparation method thereof
The invention provides a fat acellular matrix hydrogel material and a preparation method thereof. The fat freeze-dried powder is subjected to decellularization, digestion, acid-base balance regulation and gelation to prepare the fat freeze-dried powder. According to the method disclosed by the invention, a chemical decellularization method that liquid nitrogen freeze crushing is combined with 0.5% SDS and 1% Triton X-100 compound decellularization liquid is adopted, and fat is further removed through isopropyl alcohol treatment; adipose tissues can be uniformly crushed into powder through liquid nitrogen freezing and crushing, the action area of decellularization liquid is increased, the thoroughness of decellularization is remarkably improved, and the problem of fat or decellularization liquid residue can be avoided through multiple times of pure water and alcohol replacement treatment; according to the method, the mild decellularization solution is adopted for treatment for 12 hours, so that damage to key components of the matrix due to high-concentration chemical reagents and long-time action is effectively avoided. According to the method disclosed by the invention, immunological rejection caused by cell residues and adverse effects of fat residues on biocompatibility and mechanical properties can be remarkably reduced, so that the clinical applicability of the hydrogel is improved.
Owner:PEKING UNIVERSITY THIRD HOSPITAL (THE THIRD CLINICAL MEDICAL SCHOOL OF PEKING UNIVERSITY)
Regenerative tissue repair product derived from decellularized animal liver and method of making
A regenerative tissue repair product and method of making includes a decellularized extracellular matrix derived from animal liver. The decellularized extracellular matrix retains the collagen morphology of the animal liver prior to decellularization and having less than 500 ng DNA / mg dry extracellular matrix, greater than 760 microgram total collagen per milligram, the collagen comprising soluble and insoluble collagen, and greater than 1.6 μg glycosaminoglycans per milligram of dry extracellular matrix.
Owner:RTT MEDICAL INC
Implantable acellular matrix material and preparation method thereof
The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.
Owner:深圳市迈捷生命科学有限公司
Acellular-based wound dressing
PCT designated stage expiredWO2025027372A1Absorbent padsTissue regenerationWound dressingTissue sample
A method of forming an acellular sample is disclosed. The method includes forming a submucosal-free tissue and decellularization of cells existed among an interconnected framework of the submucosal-free tissue by applying a cold atmospheric plasma to the submucosal-free tissue. Forming the submucosal-free tissue includes decellularization of a submucosal layer of a tissue sample by soaking the tissue sample in an enzyme solution. The acellular sample is used for producing a wound dressing patch and a wound dressing gel.
Owner:ROSHANGAR ZINEH LEILA
Valve preparation method
The invention discloses a valve preparation method. The method comprises the following steps: performing decellularization treatment on a bovine pericardium material to be treated; enhancing the porosity of the decellularized bovine pericardium material through a physical and / or chemical method so as to promote the regeneration of native valve leaflet cells and tissues, and obtaining a composite material; and cutting the leaflet of the composite material to obtain the valve. The bovine pericardial valve which is high in durability, free of anticoagulation, excellent in biocompatibility, excellent in calcification resistance and low in immunogenicity can be prepared, and therefore the purpose of treating heart valve diseases is achieved.
Owner:KOKA NANTONG LIFESCIENCES CO LTD
Establishment of a human decellularized ovary bank for regenerative medicine and culture of immature follicles of cryopreserved ovaries
PCT designated stageWO2026062625A2Cell culture supports/coatingCell culture active agentsDecellularizationFertility preservation
The present invention discloses a comprehensive methodology for engineering a functional artificial ovary through precision tissue engineering approaches, specifically designed to overcome the limitations of current fertility preservation techniques. The present invention specifically targets the fabrication of a biomimetic artificial ovary using decellularization-recellularization technology
Owner:UNIV UTE
BME-SIS biological membrane as well as preparation method and application thereof
The invention belongs to the technical field of medicines, and particularly relates to a BME-SIS biological membrane as well as a preparation method and application thereof. The BME-SIS biological membrane is provided through a screening preparation process based on an SIS material and through composite culture and decellularization treatment of the SIS material and BMSCs. The BME-SIS biological membrane has a good decellularization effect, and avoids the problem of insecurity caused by xenograft / allograft; compared with an SIS material, the BME-SIS biological membrane has the effects of remarkably promoting cell adhesion, proliferation and differentiation, is applied to a rat rotator cuff tearing model, is proved to have the effect of remarkably promoting rotator cuff tearing healing in multiple aspects of molecular biology, histology, motility, mechanics, imaging and the like, is beneficial to reducing the retearing risk after the rotator cuff operation, and has a good application prospect. And the treatment effect is stable. The BME-SIS biological membrane disclosed by the invention can be used for bioremediation patches, and has a good prospect in the aspect of rotator cuff tear healing.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
Preparation method of tendon tissue scaffold based on decellularization technology
The invention belongs to the field of tendon tissue scaffold preparation, and provides a tendon tissue scaffold preparation method based on a decellularization technology. Although the existing decellularization technology can realize removal of cell components and retention of a matrix structure, contradiction still exists in the aspects of considering decellularization efficiency and matrix integrity. The method comprises three steps of freeze thawing treatment, trypsin digestion ultrasonic treatment and nuclease treatment, and perfect balance between decellularization efficiency and matrix retention is realized by optimizing freeze thawing parameters, regulating ultrasonic conditions and reasonably matching enzyme types and concentrations. According to the method, the cell structure can be accurately destroyed, the release of cell contents is promoted, and meanwhile, the extracellular matrix structure and bioactive molecules are protected. Experiments show that the tendon tissue scaffold prepared by the invention is thorough in cell removal, retains an extracellular matrix structure of natural tendons to the greatest extent, is closer to natural tissues in mechanical properties and biological functions, and provides a new thought for tendon repair research and application.
Owner:HEBEI UNIV OF ENG
Decellularized artificial lymph drainage tube as well as preparation method and application thereof
The invention discloses a decellularized artificial lymph drainage tube and a preparation method and application thereof, and the preparation method of the decellularized artificial lymph drainage tube comprises the following steps: taking an animal blood vessel as a raw material, and performing decellularization treatment, cleaning treatment, enhancement treatment, hole forming treatment and drying treatment on the raw material. The decellularized artificial lymph drainage tube prepared by the preparation method can solve the problems of long consumed time and difficult operation of the existing lymphatic vessel-vein anastomosis operation, can drain the lymph at the swelling part of a patient to the vein system in time after implantation, and can quickly relieve the swelling symptom. The decellularized artificial lymph drainage tube supports ingrowth, migration and proliferation of autologous cells and is good in biocompatibility, the degradation time can be adjusted to be matched with the reconstruction time of an autologous lymphatic reflux system, and long-term patency is guaranteed.
Owner:SHENZHEN QIKANG MEDICAL DEVICES
Preparation method and application of adjustable porous fat foam
The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.
Owner:THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
Decellularization device for tendon repair material
ActiveCN223906828UBioreactor/fermenter combinationsBiological substance pretreatmentsRepair materialStructural engineering
The utility model discloses a tendon repair material decellularization device which comprises a fixed top plate, the lower surface of the fixed top plate is fixedly connected with a supporting rod, the lower surface of the supporting rod is fixedly connected with a bottom plate, the upper surface of the bottom plate is fixedly connected with a protection box body, a rotating round block is arranged in the protection box body, and the rotating round block is fixedly connected with the bottom plate. A circular block groove is formed in the upper surface of the rotating circular block, an interaction container is arranged on the surface of the circular block groove, a limiting circular plate is arranged over the rotating circular block, and an electric push rod is fixedly connected to the lower surface of the fixed top plate. Through the structure, the limiting circular plate, the rotating circular block and the interaction container are arranged, the limiting circular plate can be moved to extrude and limit the interaction container on the rotating circular block, and the rotating circular block is rotated to drive the interaction container to quickly rotate, so that tendon decellularization in the interaction container is in full contact with a decellularization solution, and the decellularization efficiency is effectively improved.
Owner:BEIJING KEJIAN BIOTECHNOLOGY CO LTD
Application of ultrasonic response piezoelectric collagen scaffold
PendingCN119488638AImprove regenerative abilitypromote repairTissue regenerationProsthesisUltrasound stimulationCollagen scaffold
The invention relates to the field of bone tissue engineering and the field of biomedical materials, in particular to application of an ultrasonic response piezoelectric collagen scaffold. According to the invention, full-thickness pigskin is used as a raw material, hair and subcutaneous adipose tissue are removed, and then decellularization is carried out so as to obtain the membrane-like scaffold. The membrane-like scaffold retains a complete three-stage structure of natural collagen, which is a source of the natural collagen having a piezoelectric effect; meanwhile, a piezoelectric signal detection result shows that periodic piezoelectric signals appear on the membrane-shaped stent, and then the collagen stent provided by the invention is proved to have an intrinsic piezoelectric effect. Experimental results show that mesenchymal stem cells can be attached to the surface of the collagen scaffold and can promote repair and regeneration of bone tissues, and in addition, rat subcutaneous ectopic bone regeneration experiments prove that the collagen scaffold provided by the invention has a good bone regeneration promoting effect under ultrasonic stimulation.
Owner:SHANDONG UNIV
Metformin-induced stem cell-derived extracellular matrix material, and preparation method and application thereof
The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.
Owner:SUZHOU UNIV
Nerve graft systems, devices, and methods
Systems, devices, and methods for treating a nerve injury in a patient are provided. A system for treating a patient includes at least one of a nerve graft-conduit or nerve cap-graft comprise a nerve segment derived from a tissue source. Methods for producing a medical device comprising a nerve segment, for obtaining an ideal degree of decellularization of a nerve segment, and for treating a patient with a medical device comprising a nerve segment are provided.
Owner:RENERVA LLC
Nerve conduit from decellularized plant that can be used with or without any drug release potential
PCT designated stageWO2026063889A1Tissue regenerationLiposomal deliveryPharmacy medicinePharmaceutical drug
The present invention relates to a nerve conduit obtained by plant decellularization, which does not require 3D printing, is multi-channel, low-cost and capable of drug release. This nerve conduit is functionalized with GelMA and has properties that promote nerve healing.
Owner:ISTANBUL UNIVSI CERRAHPASA REKTORLUGU