Acellular dermal matrix and preparing method of acellular dermal matrix

An acellular dermis and matrix technology, applied in the field of medical materials, can solve the problems of limited scope of application and application, the preparation method is not described in detail, and the components of cell debris cannot be effectively removed.

Active Publication Date: 2015-12-09
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing patents disclose various preparation methods of decellularized dermal matrix. For example, the patent application whose publication number is CN104288837A discloses a method for decellularizing ultra-thin papillary skin by simply using lye, but the decellularized dermal matrix The scope of application and application of the method are very limited; the patent application with the publication number CN1775189 uses mammalian skin to be treated repeatedly with sodium hydroxide solution to remove cell genetic material DNA in the tissue, and then use detergent to remove lipids in the cell membrane, However, the cell debris components cannot be effectively removed; the patent with the publication number CN201350162Y discloses an acellular allogeneic dermal matrix tissue patch, but its preparation method is not described in detail

Method used

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  • Acellular dermal matrix and preparing method of acellular dermal matrix
  • Acellular dermal matrix and preparing method of acellular dermal matrix
  • Acellular dermal matrix and preparing method of acellular dermal matrix

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preparation example Construction

[0027] Such as figure 1 The method for preparing acellular dermal matrix includes the following steps:

[0028] S10, pretreating the skin of the mammal to obtain a dermal layer skin sheet.

[0029] The operation of pretreating the mammalian skin to obtain a dermal layer skin sheet is: mechanically degreasing and depilating the mammalian skin, and then using a skin machine to remove the 0.15mm~0.4mm epidermis from the mammalian skin After being combined with the subcutaneous tissue, a dermal skin sheet with a thickness of 0.3mm to 1.0mm is formed.

[0030] The skin of a mammal includes fur, epidermal layer, dermis layer and subcutaneous tissue layered in sequence.

[0031] Specifically, the skin of a mammal is the skin of a pig, cow, sheep, or horse.

[0032] The obtained dermal layer skin sheet can be stored at low temperature for later use. Specifically, the obtained dermal layer skin sheet can be stored at -20°C for later use.

[0033] S20: Perform degreasing treatment, virus inactiv...

Embodiment 1

[0057] (1) Preparation of dermal layer skin: the whole abdomen and back skin of healthy pigs are preliminarily degreased by mechanical method, the hair is removed with tweezers, and the subcutaneous tissue of the pig skin is removed with an electric peeling machine, and then the epidermis is removed 0.4mm, made into a dermal skin sheet with a thickness of 0.3mm, stored at -20℃ for later use;

[0058] (2) Degreasing treatment: primary degreasing is to use sodium hydroxide with a mass concentration of 1.0% for degreasing for 24 hours, and the volume ratio of the skin sheet to the degreasing agent is 1:3; the second degreasing is to place the skin sheet in the organic solvent n-ethane, Perform secondary degreasing in ethanol, with normal ethane treatment time of 60min and ethanol treatment time of 30min; the decellularized dermal matrix after degreasing is washed with phosphate buffered saline solution for 2h, and the phosphate buffered saline solution is changed every 20min;

[0059]...

Embodiment 2

[0068] (1) Preparation of dermal layer skin: the whole abdomen and back skin of healthy pigs are preliminarily degreased by mechanical method, the hair is removed with tweezers, and the subcutaneous tissue of the pig skin is removed with an electric peeler, and then the epidermis is removed 0.16mm, made into a dermis layer with a thickness of 0.7mm, stored at -20℃ for later use;

[0069] (2) Decellularization treatment: The dermis prepared in step (1) is treated with pepsin for 12 hours, and then treated with sodium dodecyl sulfate (SDS) for 24 hours, and this step is repeated 3 times;

[0070] (3) Degreasing treatment: primary degreasing is to use sodium bicarbonate with a mass concentration of 3.0% for degreasing for 12 hours, and the volume ratio of the skin piece to the degreasing agent is 1:4; the second degreasing is to put the skin piece in the organic solvent isopropanol, Perform secondary degreasing in ethanol, isopropanol treatment time is 60min, ethanol treatment time is...

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Abstract

The invention discloses an acellular dermal matrix and a preparing method of the acellular dermal matrix. The preparing method of the acellular dermal matrix comprises the following steps of: performing pre-processing on the skin of a mammal to obtain a skin corium flap; respectively performing degreasing processing, virus inactivation processing and immunogenicity removal processing on the skin corium flap to obtain an acellular dermal matrix precursor (the immunogenicity removal processing operation comprises the step of alternately soaking the skin corium flap into a prolease solution and a surfactant solution); and sequentially performing dewatering, freeze-drying and shaping on the acellular dermal matrix precursor to obtain the acellular dermal matrix. The preparing method of the acellular dermal matrix has the advantages that the skin corium flap is alternately soaked in the prolease solution and the surfactant solution, so that the immunogenicity of the acellular dermal matrix can be reduced to the maximum degree; the normal collagen structure of the acellular dermal matrix is also remained; the prepared acellular dermal matrix has the proper degradation speed, good biocompatibility and good bone induction capability.

Description

Technical field [0001] The invention relates to the field of medical materials, in particular to an acellular dermal matrix and a preparation method thereof. Background technique [0002] The source of autologous skin and allogeneic skin is extremely limited, and heterogeneous (such as: pig, cow, etc.) acellular dermis has become a major topic of soft tissue repair technology today. The dermal tissue is prone to a series of immune rejection reactions, mainly from cells and fat. There are many methods for preparing acellular dermal matrix, the purpose is to remove all the cellular components in the dermis and to maximize the integrity of the collagen structure in the dermis. [0003] Existing patents disclose the preparation methods of various acellular dermal matrices. For example, the patent application with publication number CN104288837A discloses a method for decellularizing ultra-thin papillary skin using lye, but the dermal matrix is ​​decellularized The scope of applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60
Inventor 李丽花钟梅玲谭荣伟佘振定
Owner SHENZHEN LANDO BIOMATERIALS
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