12results about How to "Improve modification efficiency" patented technology

The invention relates to the technical field of biology, in particular to a method for regulating and controlling the methylation level of a plant genome DNA specific region by using a Cas12i system. The invention provides a fusion protein. The fusion protein sequentially comprises a first nuclear localization signal peptide, a methylation modification structural domain, a connecting peptide, a nuclease activity-free Cas12i protein and a second nuclear localization signal peptide from an N terminal to a C terminal, the methylation modification structural domain is OsSUVH2 protein capable of up-regulating the methylation level or a functional active fragment of the OsSUVH2 protein; the amino acid sequence of the OsSUVH2 protein is as shown in SEQ ID NO: 1; the amino acid sequence of the nuclease activity-free Cas12i protein is as shown in SEQ ID NO: 3. The invention also provides a CRISPR-dCas12i system constructed on the basis of the fusion protein. According to the CRISPR-dCas12i system, the methylation modification efficiency of the plant genome DNA is remarkably improved.

The invention belongs to the field of agricultural fertilizers, and provides a micro-mineral activator and an application thereof in improving the fertilizer efficiency of a bio-organic fertilizer. According to the invention, epoxy silane coupling modified potassium feldspar powder is adopted as a core to construct a two-phase collaborative design of humic acid coated core-shell composite powder IM-X and metal ion crosslinked microgel powder IM-Y; the balance between the compressive strength of the particles and the rapid disintegration and dispersion performance when encountering water is realized, the consideration of a high-solid-content low-water-adding-amount granulation process window and the rheological stability of a humic acid cross-linked network is considered, and the unification of moisture absorption and caking resistance during storage and transportation and water absorption and retention ion response regulation and control functions after application is realized; the mineral-organic composite additive solves the performance coupling contradiction of natural conflicts in physical and chemical mechanisms of the existing mineral-organic composite additive, has the advantages of good processing controllability, excellent storage and transportation stability and remarkable fertilizer efficiency improvement effect, and has wide application value in the field of bio-organic fertilizer synergism.

The invention discloses a flexible bamboo lignin-based polymer material as well as a preparation method and application thereof. Arginine modified polyphenol bamboo lignin and lipoic acid are used as raw materials, and the flexible bamboo lignin-based polymer material with excellent performance is prepared through lipoic acid ring opening polymerization and sulfydryl free radical-polyphenol Michael addition reaction. The preparation method specifically comprises the following steps: carrying out demethylation phenolation modification on bamboo lignin by using a one-pot method, grafting arginine, improving the reaction activity of the bamboo lignin, quantitatively introducing lipoic acid, blocking by using allylurea, and synthesizing the bamboo lignin-based polymer material containing the allylurea, wherein the polymer material contains a dynamic disulfide reversible ring-opening structure. The method breaks through the limitation that the existing lignin is low in reaction activity and lignin-based macromolecules are difficult to recycle, expands the application scene and range of the lignin, and realizes a green mode of oriented preparation, cyclic processing and efficient utilization of the lignin-based macromolecules; and a theoretical basis and guiding significance are provided for high-quality development and high-valued frontier application of the forest resource lignin.

This invention discloses a method for preparing functional carbon materials with atomically dispersed Lewis acid-base sites, comprising: (1) polymer preparation to obtain a polymer sample; (2) polymer modification to obtain a modified polymer sample; (3) polymer carbonization: the modified polymer sample is heat-treated in a non-oxidizing atmosphere to carbonize the modified polymer sample; after carbonization, the system is naturally cooled; then, after washing and drying, the target functional carbon material is obtained. This invention utilizes atomically dispersed Lewis acidic metal sites and basic N sites to modify carbon materials in situ. The prepared functional carbon material has a large specific surface area, well-developed pore structure, abundant acid-base sites, and an atomically distributed structure, which can significantly improve the density of functional sites on the surface of the carbon material and its adsorption efficiency for boron, phosphorus, and metal impurities. Its application in the purification of chlorosilanes can greatly reduce the load of subsequent distillation and impurity removal, and improve the yield and quality of high-purity chlorosilanes.

The utility model discloses a computer keyboard tray push-and-pull device which comprises a computer desk and a keyboard tray which is arranged below the computer desk and can linearly move back and forth with the computer desk, and further comprises a push-and-pull device and a U-shaped installation support, the push-and-pull device comprises a sliding rail assembly and a sliding groove, the sliding rail assembly is fixedly connected with a computer desk panel through the U-shaped installation support, and the sliding groove is fixedly connected with the computer desk panel. The bottom wall of the sliding groove is fixedly connected with the left side face and the right side face of the keyboard tray, and the sliding groove is inserted into the sliding rail assembly and connected with the sliding rail assembly in a front-back linear moving mode. The U-shaped installation support comprises a fixing plate, a U-shaped groove plate and a pressing screw rod. By arranging the U-shaped mounting bracket, the bottom mounting mode of the keyboard tray is changed into a left and right side mounting mode, so that the stability is improved, the keyboard tray can be conveniently mounted and dismounted, a common office table is changed into a computer table without drilling into the lower part of a table panel, the labor intensity of an operator is low, and the U-shaped mounting bracket is simple in structure and convenient to process; the cost is low.

The utility model relates to a barite powder modifying and activating device for drilling fluid, and belongs to the technical field of barite powder processing. The device comprises a modification mixing bin, a material dispersing mechanism and a convection mechanism, air inlet holes are formed in the side face of the modification mixing bin, and a feeding mounting pipe is arranged at the upper end; the material dispersing mechanism is composed of a material distributing assembly and a material dispersing driving part, the material distributing assembly is located in the feeding installation pipe and is driven by the material dispersing driving part to reciprocate, it is guaranteed that barite powder is evenly dispersed into the modification mixing bin, local accumulation is avoided, and it is guaranteed that particles make full contact with a modifier; the convection mechanism comprises a convection assembly and is installed in the air inlet, airflow circulation is formed by generating pushing force, the uniform distribution condition of the barite powder in the modifier is remarkably improved through combination of the material dispersing mechanism and the convection mechanism, dynamic contact between the barite powder and the modifier is promoted, interaction between the barite powder and the modifier is further enhanced, and the service life of the modifier is prolonged. The modification is more thorough and consistent, and the modification efficiency and quality are improved.

The method for configuring and generating the tool set by the remote data conversion unit based on the ICD comprises the following steps of: extracting all XML (Extensible Markup Language) documents from the ICD, and storing the XML documents into a hash table; analog discrete magnitude configuration information, digital signal configuration information and data conversion configuration information are extracted from the hash table, and an analog discrete magnitude configuration file, a digital signal configuration file and a data conversion configuration file in an XML format are generated; and defining the format of a remote data conversion unit capable of loading a binary configuration file, and filling the data in the analog discrete magnitude configuration file, the digital signal configuration file and the data conversion configuration file in the XML format into the loadable binary configuration file. According to the method, the loadable binary file is generated to be read by the airborne software, and the source code of the airborne software does not need to be modified, so that the maintenance cost of the software is reduced, meanwhile, the link of manually modifying the source code is avoided, and the modification efficiency and accuracy are improved.

The application provides a page layout method and device, electronic equipment and readable storage medium, and belongs to the technical field of Internet. The application obtains a menu list of a webpage, wherein the menu list records at least one menu option contained on the webpage; detects operation information acting on the webpage, and determines user attention and user familiarity corresponding to each menu option based on the operation information; determines layout information corresponding to the webpage based on the user attention and the user familiarity; and arranges at least one menu option contained on the webpage based on the layout information. According to the application, the layout of the webpage can be intelligently modified according to the operation of the user on the webpage, and the efficiency of modifying the layout of the webpage is improved.

The invention discloses a process formula optimization method and semiconductor process equipment. When the method is applied to a controller of semiconductor process equipment, the method comprises the following steps: receiving a modification instruction for a process parameter fine tuning coefficient value in a process formula sent by a user side, and storing a modification value of the process parameter fine tuning coefficient value in the modification instruction in local equipment, the dynamic process parameter value is a function about the process parameter fine tuning coefficient value; after each wafer in the process task enters the process chamber, the stored modification value is read from the local device, process processing is carried out on the wafer based on the modification value, each wafer is bound with a path, a process formula is added in the path, and the path comprises the process chamber. The method is easy to manage, process task execution errors are not prone to being caused, and the modification efficiency is improved.

The utility model discloses a modification device for paperboard adhesive batching, which comprises a material tank and a modification mechanism arranged on the material tank, a redirection mechanism and a heating mechanism are arranged in the material tank, the material tank comprises a tank body, two ends of the tank body are respectively connected with an input pipe and an output pipe, and the tank body is of a hollow cavity structure. The modification mechanism comprises a modification motor and an extension shaft fixedly connected with the output end of the modification motor, multiple sets of blades are fixed to the extension shaft, the redirection mechanism comprises an annular cover, and multiple sets of redirection through holes are formed in the annular cover. According to the modification device for paperboard adhesive batching, the modification mechanism and the redirection mechanism are arranged in the modification tank, movement of materials is intensified, the movement range of the materials is wider, the modification efficiency is improved, only the materials are heated within a short time through the arranged heating mechanism, the materials cannot be continuously and excessively heated, and the modification efficiency is improved. Therefore, the modified paperboard adhesive has better properties.

The invention discloses a preparation method and application of a polymer composite material based on fly ash, and the preparation method comprises the following steps: taking fly ash and a modified substance as raw materials, and carrying out radiation grafting modification treatment to obtain modified fly ash; and carrying out melt blending on the modified fly ash and polymer granules, and carrying out extrusion granulation to obtain the fly ash-based polymer composite material. The modified substance comprises one or more of polysiloxane, a vinyl functional monomer, polyethylene and polylactic acid; the coal ash is modified through radiation, active sites capable of initiating chemical reaction are generated in the coal ash, a grafted chain segment of the obtained modified coal ash and a polymer pellet chain segment are effectively entangled together in the melt blending process, the interfacial compatibility of the coal ash and polymer pellets is improved, the mechanical property of the obtained polymer composite material is good, and the polymer composite material can be widely applied to the field of polymer composite materials. And high-value utilization of the solid waste fly ash is realized.