The invention relates to the technical field of conductive membranes, particularly to a nano-silver flexible conductive membrane and a preparation method thereof. The nano-silver flexible conductive membrane comprises a PET substrate layer, wherein corona treatment is performed on the surface of the PET substrate layer; a conductive coating layer which is arranged on the surface of the PET substrate layer comprises the following raw materials in percentage by weight: 50-75% of absolute ethyl alcohol, 2-5% of terpilenol, 1-3% of acetyl tributyl citrate, 2-8% of joint cement, 1-5% of polyethylene glycol 400, 2-3% of span 85, 1.5-3% of ethyl cellulose, 3-8% of silver powder of 20-40 nanometers, and 10-18% of silver powder of 150-450 nanometers. The nano-silver flexible conductive membrane, provided by the invention, has the characteristics of stable surface conductivity of the formed film, low resistivity, high light transmittance and strong adhesive force. The preparation process of the nano-silver flexible conductive membrane is simple and mature, coating equipment is simple, the operation is convenient, the cost is low, and the nano-silver flexible conductive membrane facilitates popularization and application.