161 results about "Hickory nut" patented technology

The invention relates to the technical field of molecular markers, in particular to KASP primers of 112 carya illinoensis varieties, an SNP molecular marker, a fingerprint spectrum and application of the KASP primers and the SNP molecular marker. By adopting the technical scheme provided by the invention, the carya illinoensis fingerprint spectrum can be drawn, is used for accurate identification of carya illinoensis varieties, and has important significance on germplasm resource identification and molecular marker breeding of carya illinoensis.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker and a DNA (Deoxyribose Nucleic Acid) bar code of 'Zhenxiu 28' carya illinoensis and The DNA bar code or the SNP molecular marker can be used for identifying the 'Zhenxiu 28' carya illinoensis, and is of great significance to germplasm resource identification and molecular marker breeding of the carya illinoensis.

The invention provides an isothermal amplification primer probe set for detecting Colletotrichum fucicola of Carya illinoensis, a rapid detection kit, a method for detecting Colletotrichum fucicola and application of the isothermal amplification primer probe set and the rapid detection kit, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current apocarya colletotrichum gloeosporioides is difficult to identify, excessively depends on expensive instruments and technicians and the like, the apocarya colletotrichum gloeosporioides LFD-RPA detection technology research is developed, the apocarya colletotrichum gloeosporioides can be simply, conveniently and efficiently detected, the apocarya colletotrichum gloeosporioides LFD-RPA has no cross reaction with 22 representative strains of the colletotrichum gloeosporioides, the specificity is high, the detection time is the lowest detection limit is 10 pg of genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker of Yaolu No.3 carya illinoensis and application of the SNP molecular marker. The SNP molecular marker disclosed by the invention can be used for identifying the 'Yaolu No.3' carya illinoensis, and has important significance on germplasm resource identification and molecular marker breeding of the carya illinoensis.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker of 'Zhenxiu 15' carya illinoensis and application of the SNP molecular marker. The SNP molecular marker can be used for identifying 'Zhenxiu 15' carya illinoensis, and is of great significance to germplasm resource identification and molecular marker breeding of carya illinoensis.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention provides an isothermal amplification primer probe group, a rapid detection kit, a method and application for detecting Xanthomonas vesicans of Carya illinoensis, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current carya illinoensis scab is difficult to identify, excessively depends on expensive instruments and technicians and the like, the research on the LFD-RPA detection technology of the carya illinoensis scab is developed, and the carya illinoensis scab can be simply, conveniently and efficiently detected; in addition, the kit has no cross reaction with 18 representative strains of other species and similar species (cladosporium) and other 14 genus in the genus of furazolospora, the specificity is strong, the lowest detection limit is 5 pg genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention discloses a pecan astringency removal method, which is characterized in that tannase and pecan kernels react at about 40 DEG C, so that the contents of main astringency component substances such as condensed tannin, flavone and phenolic compounds in pecan endopleura can be effectively reduced, and the original rich nutrition and unique flavor of pecan are maintained. The invention also discloses a degradation catalysis area of tannase in a deastringency process. The method provided by the invention can solve the problems existing in the existing hickory nut deastringency processing technology, retains better quality and taste of hickory nut compared with the traditional water boiling hickory nut deastringency, still retains partial substances such as saccharides, soluble protein, fatty acid and the like with nutritional value while deastringency, reduces water consumption, reduces wastewater pollution, and is suitable for industrial production. And the green and healthy processing purpose is achieved.

The invention belongs to the technical field of carya illinoensis cultivation. The invention discloses a high-yield apocarya tree shape culture method, which achieves the purpose of high-yield tree shape rapid forming by fixing a trunk 1-2 years after planting fruiting large container seedlings, culturing a first layer of branches, controlling a first layer of main and lateral branches 3-4 years after planting, culturing a second layer of branches, controlling the first and second layers of main and lateral branches 5-6 years after planting, and culturing a third layer of branches. The tree shape culture period is shortened, and the high yield of carya illinoensis fruits is accelerated.

The invention belongs to the field of forestry, and particularly relates to a carya illinoensis big tree variety optimization and rapid yield increasing method which comprises the steps of variety selection and configuration, scion collection and preservation, big tree treatment, grafting and post-grafting management. According to the method, multi-head layered grafting is adopted for grafting, new varieties suitable for localized development (which is approved (recognized) by the national or provincial forest and grass improved variety certification committee and is suitable for local development) are used for optimizing original varieties, and integration of a rapid high-yield cultivation technology is matched; compared with other grafting methods (such as stem-cutting grafting and an existing layered grafting technology), the survival rate is increased by 30% or above, and the cultivation period before fruiting is shortened by 3 years or above. According to the method, the scions are treated in a dry ice sand blasting-room-temperature water buffering-low-temperature water heat shock mode, the scion rescue rate can be remarkably increased, and meanwhile the fruit malformation rate of first fruiting of the scions is not affected.

The invention discloses an apocarya source antioxidant active peptide as well as a screening method and application thereof. Three unreported antioxidant active peptides WDPFE, FDPFP and WDPNY with antioxidant activity are screened from carya illinoensis protein zymolyte for the first time, the carya illinoensis antioxidant peptides can improve the activity of human epithelial cells Caco-2 under the oxidative stress condition, the levels of antioxidant enzymes SOD and CAT in the cells are remarkably improved, and the antioxidant activity of the carya illinoensis is remarkably improved. And the compound is safe, non-toxic, good in water solubility and high in biological activity, can be widely applied to research and development of functional foods, medicines and health products, and has important economic value and social significance.

Methods of farming, domesticating, or cultivating truffle fungi, including isolation and cultivation methods for producing pure truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame, sunflower, grapes, blackberries, blueberries, cherries, kiwi, mango, raspberries, and huckleberries.

The invention discloses a carya illinoensis oil shelf life prediction model and a construction method thereof, the initial peroxide value, the storage temperature and the final peroxide value of carya illinoensis oil are input into the carya illinoensis oil shelf life prediction model, and the shelf life of cold-pressed carya illinoensis oil or hot-pressed carya illinoensis oil can be predicted; the construction method comprises the following steps: firstly, storing an oil sample at a constant temperature and measuring a peroxide value, secondly, substituting the peroxide value into a primary reaction kinetic equation to obtain a peroxide value change rate constant, and finally, substituting the peroxide value change rate constant into an Arrhenius equation to obtain a peroxide value shelf life prediction model of the carya illinoensis oil. The carya illinoensis oil shelf life prediction model provided by the invention can predict the shelf life of cold-pressed or hot-pressed carya illinoensis oil stored at 20-60 DEG C, the prediction accuracy can reach 80% or above, and the carya illinoensis oil shelf life prediction model has the characteristics of real-time performance, high efficiency and accuracy, and provides a theoretical basis for evaluating the stability and quality control of the carya illinoensis oil.

The invention provides a rapid orchard building method for grafting after planting of carya illinoensis large stock seedlings, and belongs to the technical field of fruit tree grafting. The method for grafting and quickly building the orchard after planting the large carya illinoensis stock seedlings comprises the following steps: (1) transplanting and planting the large carya illinoensis stock seedlings as grafting stocks; the diameter at breast height of the carya illinoensis large seedlings is 6-9 cm; (2) collecting scions; (3) grafting from the last ten days of April to the first ten days of May, cutting the rootstock at a position 1-1.2 m above the ground, grafting by adopting a bark insertion method, and symmetrically grafting two scions on each rootstock; (4) management after grafting. After the garden is built, the carya illinoensis begins to bear fruits in the third year (the carya illinoensis enters the fruiting period two years earlier than grafting seedling garden building), high yield is achieved in 6-7 years (the carya illinoensis enters the high-yield period four years earlier than grafting seedling garden building), and the garden building efficiency of the carya illinoensis is greatly improved.

The invention discloses a nano-cellulose modified thermoplastic material composite 3D printing consumable and a preparation method, and relates to the technical field of 3D printing consumables, the nano-cellulose modified thermoplastic material composite 3D printing consumable is formed by mixing nano-cellulose modified pecan shell micro powder and a thermoplastic material according to the mass ratio of 1: (4-5); the nano-cellulose modified hickory nut shell micro-powder is prepared from hickory nut shells, talcum powder, nano-cellulose, cationic starch, alkyl ketene dimer, a coupling agent and a lubricating agent. The thermoplastic material is composed of polylactic acid, poly (adipic acid) / butylene terephthalate, a cellulose biological composite material, a flexibilizer and an antioxidant, and the problems that talcum powder and hickory nut powder are not tightly combined and are prone to falling off and components are unstable are effectively solved by adopting the novel separation device; the tight combination and uniform distribution of the talcum powder and the pecan shell micro powder are ensured, so that the compatibility of the talcum powder and the pecan shell micro powder with a thermoplastic material and the overall performance of the composite material are improved.

The invention discloses a method for inducing apocarya stems to generate transgenic hairy roots, and belongs to the field of forest tree genetic engineering. According to the method, the semi-lignified or lignified stems of the apocarya are infected by utilizing the agrobacterium rhizogenes to obtain stable transgenic hairy roots, rooting can be induced within 1-2 months, the rooting rate is as high as 94.43%, and the transgenic positive rate can reach 98.38%. The invention provides an economical and efficient non-tissue culture dependent transgenic method, it is clear that transgenic roots can be used for gene function verification, the method breaks through the technical obstacle that stable genetic transformation of the carya illinoensis is difficult, and technical support is provided for research of functional genes.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The invention belongs to the technical field of hickory nut storage, and discloses a hickory nut anti-oxidation storage pretreatment process and pretreatment equipment, and the process comprises the following steps: S1, sorting freshly picked and cleaned hickory nuts through the pretreatment equipment, and removing empty and shriveled seeds, bad seeds and the like; s2, screening the hickory nuts without empty and shriveled seeds, bad seeds and the like through pretreatment equipment in a unified manner to distinguish small, medium and large hickory nuts, and respectively feeding the hickory nuts into different assembly lines for spraying antioxidant and edible coating liquid; s3, the small, medium and large carya cathayensis fruits sprayed with the coating liquid enter different intelligent controlled atmosphere all-in-one machines to be subjected to controlled atmosphere treatment, and low-temperature storage is combined, so that the storage period of the carya cathayensis fruits with shells is prolonged to 6-8 months, and the storage period of the shelled carya cathayensis kernels is prolonged to 3-4 months; wherein during the controlled atmosphere treatment, the concentration of oxygen is 2-5%, the concentration of carbon dioxide is 3-5%, and the balance is nitrogen. According to the low-oxygen environment, moisture evaporation and nutrient consumption of the nuts are reduced, the freshness of the nuts can be kept, and peculiar smells caused by oxidation are avoided.

Methods of farming, domesticating, or cultivating truffle fungi, including isolation and cultivation methods for producing pure truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame, sunflower, grapes, blackberries, blueberries, cherries, kiwi, mango, raspberries, and huckleberries.

The invention relates to the technical field of walnut oil refining, in particular to a refining method of low-temperature cold-pressed hickory nut oil, which comprises the following steps: adding magnetized water and a magnetic enzyme carrier into pretreated hickory nut crude oil to carry out enzymolysis reaction, and carrying out magnetic separation and separation after the enzymolysis reaction is finished to obtain degummed hickory nut oil; adding a modified decolorizing agent into the degummed hickory nut oil, and filtering after decolorizing to obtain decolorized hickory nut oil; wherein the modified decolorizing agent consists of activated clay and sugar carbon; and carrying out vacuum dehydration treatment on the decolored pecan oil to obtain the refined pecan oil. According to the invention, the magnetic enzyme carrier, the magnetized water and the modified decolorizing agent are matched for use, so that the pecan oil is degummed and decolorized better, and the quality of the finished pecan oil is ensured.

The invention relates to the field of disease and pest control, in particular to a disease and pest control method for pecans. The method comprises the following steps: S1, adding a prepared insecticide into an insecticide box; s2, a plurality of agent boxes are fixed to the peripheral side of a trunk of the walnut tree through hook-and-loop fasteners and binding bands; s3, smearing insect sticking glue on the peripheral surface of the hook-and-loop fastener; s4, the insecticide seeps from the bottom of the insecticide box and drips to the periphery of the bottom of the trunk, a circle of insecticide area is formed at the bottom of the trunk, and insecticide liquid drops are thrown away when wind blows; and S5, observing the remaining amount of the insecticide in the insecticide box through a transparent window on the insecticide box, and supplementing the insecticide in time when the insecticide is insufficient. The pesticide box automatically drips pesticide to achieve continuous pest and disease control, a worker only needs to add the pesticide into the pesticide box and does not need to spray the pesticide by himself / herself, and physical strength is saved.

The utility model belongs to the technical field of cutting saws, and particularly discloses a carya illinoensis big tree pest and disease damage branch harvesting device which comprises a first mounting plate, a plurality of second shaft seats are fixed on the first mounting plate, rotating shaft seats are arranged on the second shaft seats, first telescopic arms are fixed on the rotating shaft seats, and second telescopic arms are fixed on the first telescopic arms. A second telescopic arm is arranged in each first telescopic arm in a sleeved mode, a plurality of second hydraulic telescopic rods are fixed to the four corners in each second telescopic arm, third telescopic arms are arranged among the second hydraulic telescopic rods, first hydraulic telescopic rods are arranged in the third telescopic arms, and a plurality of connecting blocks are fixed to the output ends of the first hydraulic telescopic rods. A second mounting plate fixed to the end of the third telescopic arm is arranged above the connecting block, a fixing block is fixed to the second mounting plate, a rotary disc is arranged on the fixing block, a fixing plate is fixed to the rotary disc, a second motor and a rocker arm are arranged on the two sides of the fixing plate respectively, a plurality of third hydraulic telescopic rods are fixed to one side of the rocker arm, and clamping jaws are fixed to the output ends of the third hydraulic telescopic rods.

The utility model relates to the technical field of walnut planting equipment, and discloses a spraying device for planting carya illinoensis, which comprises a water storage tank, the bottom of the water storage tank is fixedly connected with a water outlet pipe, one end of the outer side of the water outlet pipe is connected with a conveying pump, and an output pipe of the conveying pump is fixedly connected with a connecting pipeline; a plurality of water injection hoses are fixedly connected to the outer side of the connecting pipeline; a plurality of spraying structures are arranged, water in a water storage tank is conveyed to a connecting pipeline through a conveying pump, then the water is distributed to vertical pipes and electric rotating nozzles through a water injection hose, a driving motor drives a transmission lead screw to rotate, a movable block moves on a sliding rail, and therefore the positions of the water injection hose and the electric rotating nozzles are adjusted; the carya illinoensis in different areas can be accurately sprayed, a plurality of fixing frame structures are arranged, large-area planting areas can be automatically irrigated at the same time, and the working efficiency is greatly improved.

The invention discloses automatic efficient cleaning equipment for hickory nuts, and belongs to the technical field of walnut cleaning, the automatic efficient cleaning equipment for hickory nuts comprises a cleaning box, a water tank is fixedly mounted on the surface of the cleaning box, a flushing mechanism is fixedly mounted in the cleaning box, and a sewage draining mechanism is fixedly mounted in the cleaning box; a brush mechanism, a discharging mechanism and a sealing mechanism are fixedly installed in the cleaning box, a plurality of damping mechanisms are fixedly installed on the surface of the cleaning box, a water pump is fixedly installed on the surface of the cleaning box, and the material washing table is arranged to be in a step shape. The hickory nuts fall slowly, the washing mechanism can wash the hickory nuts, green husks on the surfaces of the hickory nuts are left in the triangular groove after being washed and can be discharged from the interior of the cleaning box through the sewage discharging mechanism, and a water source for washing falls to the brush mechanism from the water discharging hole for secondary utilization.

The present invention relates to the technical field of molecular markers, and particularly relates to KASP primers, SNP molecular markers, fingerprint maps of 112 pecan varieties and their applications. By adopting the technical scheme of the present invention, a fingerprint map of pecan can be drawn, which is used for accurate identification of pecan varieties and is of great significance for the identification of germplasm resources of pecan and molecular marker breeding.

The invention discloses a method for planting carya illinoensis under forest and morchella esculenta, and belongs to the technical field of mushroom planting. The method specifically comprises the steps of forest land selection, deadwood and weed removal, ploughing and soil preparation treatment, greenhouse building, soil improvement, soil disinfection, ditching and ridging, morchella esculenta sowing, watering, temperature and humidity management, nutrition management, management after fruiting and harvesting. According to the method for planting the carya illinoensis under the forest and the morchella esculenta, efficient utilization of the land under the forest is achieved, cost input is reduced, planting benefits are increased, planting of the morchella esculenta under the forest in China is promoted, and a new thought is provided for development of characteristic economy under the forest and production of edible mushrooms in China.

The present invention discloses a method for inducing transgenic hairy roots in the stems of Carya serrata, belonging to the field of forest genetic engineering. The method utilizes Agrobacterium rhizogenes to infect semi-lignified or lignified stems of Carya serrata to obtain stable transgenic hairy roots. These roots can be induced to develop within one to two months, with a rooting rate as high as 94.43% and a transgene-positive rate of 98.38%. This method provides a cost-effective, tissue culture-independent transgenic method, and clearly demonstrates that transgenic roots can be used to verify gene function. This method overcomes the technical barriers to stable genetic transformation in Carya serrata, providing technical support for the research of functional genes.

Described is a method of processing nut fruits selected from almond fruits, pecan fruits and / or walnut fruits, comprising: a) off-ground harvesting of the nut fruits; b) subjecting a mass comprising kernels, inner shell and green outer hull of the nut fruits to grinding to an average particle size of 500 μm or less to provide a ground mass; and c) further processing the ground mass to edible nut fruit solids, edible nut fruit-based products and / or nut fruit beverages; wherein the nut fruits have not been subjected to drying before step b). The method provides an ecologically friendly and simple alternative for processing almond fruits, pecan fruits and / or walnut fruits to food and beverage compositions that are not only suitable for human consumption but simultaneously comprise high amounts of the natural antioxidants and bioactive materials present in the fresh fruits. Simultaneously, the method reduces risks related to both consumer health and the orchard environment, does not require extensive use of herbicides and / or pesticides, and reduces dust generation and tree water stress. In addition, edible products and beverages obtained by the aforementioned method are described.