107 results about "Cladosporium" patented technology

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

The invention relates to bidirectional burkholderia as well as a culture method and an application thereof and belongs to the field of plant protection (microbial pesticide). The preservation number of the bidirectional burkholderia is CCTCC NO: M2013145. The culture method of the bidirectional burkholderia comprises the following steps: adding 1ml of 2-day-aged seed bacterial strain to an LB (Lysogeny broth) culture medium with the liquid loading of 50ml under a fermentation condition, and culturing for 20-24 hours at the temperature of 25-30 DEG C at the rate of 200r/min. The bidirectional burkholderia has a remarkable inhibition effect on rice sheath blight disease, rice blast, colletotrichum musae, oxysporum, fusarium asiaticum, peronophythora litchi, sugarcane alternaria tenuis, cladosporium fulvum, xanthomonas oryzae, bacterial leaf steak and the like, can be used for the biological prevention and control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environmental friendliness and the like and has a bright application prospect.

The invention relates to the technical field of biology, in particular to a Cladosporium strain LJ1 and an application thereof. The invention discloses the Cladosporium strain Clodophahora sp. LJ1 with a preservation number being CGMCC NO. 18140, the strain has the ability to effectively prevent and cure banana fusarium wilt. In the resistance experiment of banana fusarium wilt, the control effectin a plate symbiosis experiment is 88.89%, and a potted experiment control effect can reach 53.67%. The strain has a broad-spectrum growth-promoting effect, which can effectively promote the growth of tomato seedlings (solanaceous plants) and dendrobium officinale seedlings (blue family plants).

This invention has clone Stilbene synthase gene (sts) from Parthenocissi Tricuspidatae endogeny eumycete, the coding sequence similarity between Stilbene synthase gene and Parthenocissi Tricuspidatae is 95.25%,this eumycete belongs to Cladosporium sp by 18SrDNA series assessment. High performance liquid chromatogram and mass chromatographic analysis indicate that this eumycete can also composite resveratrol by culture in vitro. The analysis about non- coding part of endogeny eumycete sts gene indicates that: it is very different from regulatory element of sts gene which is in plants. Importing exogenous gene(such as VHb,ipt and iaaM and so on ) in endogeny eumycete can promote endogeny eumycete growing.

The invention belongs to the technical field of biology. Lipase widely exists in animal tissues, plants and microorganisms and can catalyze hydrolysis, alcoholysis, esterification and ester interchange of ester compounds, synthesis of compounds and the like. The invention aims to provide cladosporium capable of producing organic solvent tolerant lipase, which is a cladosporium strain (Cladosporium sp.) WBRD3.10062425 and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 4962. The invention also aims to provide the organic solvent tolerant lipase produced by the strain and a preparation method thereof. The lipase produced by the strain disclosed by the invention is the organic solvent tolerant lipase, has strong tolerance for methanol, ethanol and acetone and has wide application value in various industrial fields of biodiesel, medical industry, foods, oleochemical industry, environmental protection, detergents, textile, papermaking and the like.

The invention provides application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin, and relates to the technical field of microorganisms. The application is mainly used for solving the problems that most existing steroid saponin of liriope spicata var.prolifera Y.T.MA is from extracts of liriope spicata var.prolifera Y.T.MA, tubers of liriopespicata var.prolifera Y.T.MA are long in production period, low in steroid saponin content and high in saponin extraction cost, and accordingly the market volume of the steroid saponin of liriope spicata var.prolifera Y.T.MA is limited. The fungus is separated from living tubers of liriope spicata var.prolifera Y.T.MA, authenticated as Cladosporium sp.EF111 by means of the 18 S rDNA sequence, andpreserved in the China Center for Type Culture Collection. The preservation date is December 7th, 2016, and the preservation number of CCTCC No:M2016725. According to the application, liriope spicatavar.prolifera Y.T.MA produced by a liriope spicata var.prolifera Y.T.MA GAP base which is the original production place of liriope spicata var.prolifera Y.T.MA in Oumiao town, Xiangyang city in Hubeiprovince, endophytic fungus strain liquid of liriope spicata var.prolifera Y.T.MA is used for fermentation, and the steroid saponin of liriope spicata var.prolifera Y.T.MA is produced. The endophyticfungus is an important microorganism for finding new resources of steroid saponin of liriope spicata var.prolifera Y.T.MA.

A novel process for the remediation of nitrogenous energetic materials such as 1,3,5,-trinitro-1,3,5-triazine (RDX) which can be used in situ on contaminated media is provided. The process comprises the bioremediation by one or more miccroorganisms capable of metabolizing the energetic materials. Examples of such microorganisms include Rhizobium rhizogenes, Burkholderia sp., and Cladosporium cladosporioides (ATCC 66669). Strains of these microorganisms have been deposited. The strain designated A1 has been deposited as Rhizobium rhizogenes BL (ATCC PTA-4110) and the strain designated C8 has been deposited as Burkholderia sp. (ATCC PTA-4111) Additionally, with the addition of a carbon source, such as a sugar, the process can totally degrade the energetic materials in two to three days.

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

The invention discloses a use method of drugs for preventing and treating cladosporium cucumerinum. The use method comprises the following steps: A, preparing a raw solution which comprises the following raw materials: 50% of carbendazim, 70% of thiophanate methyl, a new high lipid membrane, a fruit and vegetable base promoter, astragalus polysaccharides, glycyrrhizic acid, radix isatidis and fructus forsythia; B, soaking tomato seeds subjected to greenhouse culture in warm water at the temperature of 55 DEG C for 30 minutes to remove pathogenic bacteria inside and outside the seeds, taking out, cooling in cold water, soaking in the raw solution with the volume being 400 times that of the seeds for 1.5-2 hours, taking out, washing by clear water, drying in the sun, accelerating germination, and seeding; C, during an attack, adding water into the raw solution for diluting the raw solution by 600 times, and spraying; D, while spraying in the step C, adding water into the raw solution for diluting the raw solution by 500 times, and carrying out root irrigation. The use method is simple; the used drugs are simple in ratio, convenient to use and good in treatment effect on tomato leaf mould; a test shows that a mixture diluted by 400-600 times has a remarkable deactivation effect on deuteromycotina-cladosporium fulvum and has a good sterilization effect.

The invention discloses a probe and a kit for the common pathogens detection of skin infectious granuloma. The probe comprises a mycobacterium probe, a mycobacterium tuberculosis probe, a mycobacterium leprae probe, a mycobacterium avium probe, a mycobacterium intracellulare probe, a mycobacterium kansasii probe and a mycobacterium fortuitum probe, a candida albicans probe, a sporotrichum schenckii probe, a cladosporium trichoides probe, a Fonsecaea pedrosoi probe, and a Fonsecaea monophora probe; and a nocardia probe, an actinomycetes probe and a leishmania probe. The nucleotide sequence of each probe is shown in sequence tables SEQ ID NO.1-SEQ ID NO.15; and the kit comprises the probes and corresponding universal primers. According to the invention, through designing and optimizing the kit for carrying out pathogens screening and detection on common skin infectious granuloma, the improvement of the clinical diagnosis and the treatment efficiency is facilitated.

The invention relates to application of amomum tsaoko essential oil and an emulsion thereof as air fresheners and/or air bacteriostats. The amomum tsaoko essential oil and the amomum tsaoko essentialoil emulsion have antibacterial and bacteriostatic effects on the following harmful air microorganisms: escherichia coli, staphylococcus aureus, candida albicans and fungi of penicillium, aspergillusand cladosporium.

The present invention relates to the use of primers in polymerase chain reaction assays for the detection of fungal pathogens Colletotrichum acutatum, Alternaria spp., and Cladosporium carpophilum. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques. Also described are novel extraction buffer solutions for use in isolating DNA from an organism, methods of extracting DNA from tissue, and methods of performing PCR analysis on DNA extracted from tissue.

The invention discloses a method for obtaining ferulic acid by using wastes, and belongs to the technical field of the fermentation industry. The method comprises the following steps: mixing of distiller's grains with the bran, inoculation, cultivation for obtaining a solid fermentation product of the distiller's grains, and extraction of the ferulic acid in the solid fermentation product of the distiller's grains by a hot water extraction process. The wastes bran and the distiller's grains in the grain processing and wine brewing processes are used as raw materials, so the cost and the pollution are reduced, and the added values are increased; the ferulic acid is enriched by using Cladosporium cladosporioides, so a large class of bacteria is provided for the enrichment production of the ferulic acid to select; and further, the method has the advantages of simple fermentation production device, low capital investment, stable reaction process, convenience in implementation, and facilitation of industrial production.

The invention provides a lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity. The strain is preserved in China General Microbiological Culture Collection Center on July 6, 2020, and the preservation number is CGMCC No.20198. The lactobacillus plantarum PC28152 and the fermentation liquor thereof have the effect of inhibiting fungal activity in a broad spectrum, and particularly have an obvious inhibiting effect on aspergillus flavus, penicillium chrysogenum, penicillium expansum, fusarium moniliforme and cladosporium cladosporioides. When the strain is applied to food preservation and preservation, infection of spoilage fungi to food can be effectively inhibited, and the shelf life of the food is remarkably prolonged.

The invention relates 2-amino-1,3,4-thiadiazolethioacetamido aromatic hydrocarbon derivatives of a general formula (I) and a method for preparing the same, pesticide composition using the same as active ingredient and use of the same in the prevention, control and treatment of Cladosporium cucumerinum Ellis & Arthur, Cladosporium fulvum and the like. The derivatives are synthesized by the reaction of 2-amino-5-mercapto-1,3,4-thiadiazole activated by an alkali and acetyl chloride-substituted aromatic hydrocarbon in a polar aprotic solvent. The compounds have high bacteriostatic activity against common plant pathogenic fungi and can be used as compound preparations in antiseptic effective dosage or added into an acceptable assistant to make the pesticide composition. Particularly, the 2-amino-1,3,4-thiadiazolethioacetamido aromatic hydrocarbon derivatives can be used to make water dispersible tablets that have remarkable protective and treatment effects on the common plant pathogenic fungi such as Cladosporium cucumerinum Ellis and Arthur and Cladosporium fulvum and are high-effective, low-residue, poisonous residue-free and widely-applicable novel green nuisanceless bactericides.

The invention belongs to the field of microbial biotechnology, relates to a (cladosporium cladosporioides) Cc-106 strain for production of lovastatin through liquid fermentation and application and a fermentation production method. The Cc-106 strain is preserved in China Center for Type Culture Collection on July, 7, 2016 with the preservation number CCTCC M 2016380. The strain has the good characteristics of high yield production of the lovastatin, high sporulation quantity, simple culturing and the like. Under the conditions that the initial pH of a fermentation medium is 5.5, the culture temperature is 28 DEG C, and Cc-106 strain liquid fermentation is performed for 80h to 136h in a 5L fermentation tank, the yield of the lovastatin can reach about 184mg/L.

A method and kit of components for destroying organisms and toxins in from an enclosure such as a building. Temperature sensing probes are installed in the enclosure to indicate structure temperature and a recorder is used to record the temperature of said sensing probes in real time. Hot air is introduced into the enclosure through one or more ducts to raise the structure temperature to at least about 120° F., as monitored by the temperature sensing probes. This is sufficient to kill essentially all insects, bacteria, virus, dust mites, spiders, silver fish, fungi and toxic molds such as aspergillus oryzae, aspergillus terreus, aspergills versicolor, cladosporium hergbarum, stachybotrys chartarum, penicillium aurantiogriseum, pencillium chrsogenum, pencillium glabrum and fusarium oxysporum. and the like. The air can exit through open doors and windows or through ducts to a filter assembly that captures the remains of the organisms. Ozone may be added to the heated air to improve efficiency. Preferably, air when extracted from an egress duct downstream of the filters improves air flow through the enclosure and filters. A typical building can be treated in six to eight hours.

The invention discloses application of a perylenequinone compound in preparation of a feed for preventing and controlling ruminant viruses, and belongs to the technical field of bioengineering and agriculture. The perylenequinone compound is added into a ruminant feed to prepare the feed for preventing and controlling the ruminant viruses such as foot-and-mouth disease virus, cell pathogenetic bovine viral diarrhea virus-mucosal disease virus, bluetongue virus, capripoxvirus, epuine morbillivirus and the like. The perylenequinone compound comprises hypocrellin, elsinochrome, phleichrome, cladosporium, calphostin, cercosporin and the like; and the perylenequinone compound has a series of advantages of extracting convenience, good stability, no obvious side effect in oral administration, quick metabolism and the like. The dosage of the prepared feed for preventing the ruminant viruses is 1 to 10mumol perylenequinone compound per kg weight every day, the dosage of the prepared feed for treating the ruminant viruses is 10 to 100mumol perylenequinone compound per kg weight, the effect of preventing and treating the viruses is very remarkable, meanwhile, the perylenequinone compound can be obtained by modern biotechnology, and good economic and social benefits can be generated.

The invention discloses an application of isoliquiritin, particularly the application of isoliquiritin to plant disease prevention. Through indoor biological measurement and pot experiment, the invention provides a better preventing and treating application of isoliquiritin which is active ingredient in liquorice methyl alcohol extract to common plant disease such as Peronophythora Chen, Phytophthora capsici Leonian, Cladosporium LK. ex Fr, Sclerotinia Colletotrichum Corda and the like. The invention provides a new design for liquorice and extract thereof applied to plant disease prevention, provides a new development resource for plant substitution chemical synthesis bactericide, and has important significance for solving the problem that pathogenicbacteria has increasingly remarkable resistance to drug.

The invention discloses a cladosporium yielding cholesterase (Cladosporium sp.) DZ16 which is screened, wherein the preservation number is CCTCC M2016685. The bacterial colony of the culture is velvet-like and is activated by means of a first step of preserving and activating the culture and a second step of plate spraying of the culture. In the early stage of fermentation, the biomass of the bacterial strain is activated and cultured in a low salt liquid state in the first step. In the later stage, by means of multifactor compounding action in the second step, the bacterial strain is inducedtop secrete zymoprotein, so that the enzyme activity is improved. The cholesterase produced by fermenting the bacterial strain has a good application potential in the fields of medical detection, food, pulping and paper-making industry, environmental protection and the like.