Application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin

Abstract

The invention provides application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin, and relates to the technical field of microorganisms. The application is mainly used for solving the problems that most existing steroid saponin of liriope spicata var.prolifera Y.T.MA is from extracts of liriope spicata var.prolifera Y.T.MA, tubers of liriopespicata var.prolifera Y.T.MA are long in production period, low in steroid saponin content and high in saponin extraction cost, and accordingly the market volume of the steroid saponin of liriope spicata var.prolifera Y.T.MA is limited. The fungus is separated from living tubers of liriope spicata var.prolifera Y.T.MA, authenticated as Cladosporium sp.EF111 by means of the 18 S rDNA sequence, andpreserved in the China Center for Type Culture Collection. The preservation date is December 7th, 2016, and the preservation number of CCTCC No:M2016725. According to the application, liriope spicatavar.prolifera Y.T.MA produced by a liriope spicata var.prolifera Y.T.MA GAP base which is the original production place of liriope spicata var.prolifera Y.T.MA in Oumiao town, Xiangyang city in Hubeiprovince, endophytic fungus strain liquid of liriope spicata var.prolifera Y.T.MA is used for fermentation, and the steroid saponin of liriope spicata var.prolifera Y.T.MA is produced. The endophyticfungus is an important microorganism for finding new resources of steroid saponin of liriope spicata var.prolifera Y.T.MA.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of Ophiopogon japonicus, and the endophytic fungus is a fungus of the genus Cladosporium capable of producing steroidal saponins from Ophiopogon japonicus. Background technique [0002] Xiangmaidong steroidal saponins are national geographical indication products, authentic Chinese medicinal plants in Hubei——Xiangmaidong Liriope spicatavar. prolifera The main active ingredient of Y. T. Ma tuber root, its saponin basic skeleton belongs to the derivative of Spirostane (Spirostane, which has 27 carbon atoms in total), according to the configuration of C25 and the ring closure state of the ring in the Spirostane structure, it can be It is divided into spirostanols (C25 is S configuration), isospirostanols (C25 is R configuration), furostanols (F ring is an open-chain derivative), deformed spirostanols Alcohols (F ring is a five-membered tetrahydrofuran...

AI Technical Summary

Problems solved by technology

[0003] Although with the progress of chiral synthesis and oligosaccharide synthesis methods, there have been some reports on the total synthesis of steroidal saponin compounds, but due to the complexity of the steroidal saponin structure, the synthesis is very difficult, and the chemical synthesis is harmful to the environment. Huge pollution, currently mainly obtained directly through the separation of root extracts

However, in the actual production of Ophiopogon japonicus, there are problems such as "small planting area of ​​continuous cropping obstacles", "greatly affected by climatic conditions", "long production cycle of roots", "low content of steroidal saponins", "high cost of saponin extraction". " and other factors have led to restrictions on the market release of xiangmaidong steroidal saponins

At present, there is no report about a kind of endophytic fungus capable of producing xiangmaidong steroidal saponins through microbial fermentation and its application

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