175 results about "Steroid Saponins" patented technology

An obesity control agent with health-restorative and health-promotional benefits to humans comprising the extract of Chlorophytum species, more particularly, Chlorophytum arundinacceum, is disclosed. The bioactive principles responsible for anti-obesity property have been determined to be mainly due to spirosta-steroidal saponins, spirosta-steroidal alkaloids and galacto-glucan oligosaccharides. Most effective as an obesity control agent is the spirosta-steroidal saponins. Pharmaceutical, nutritional and veterinary use of this inventive composition is also disclosed.

The invention provides a liriope spicate endogenous aspergillus fungus and application thereof to preparation of steroid saponin, and relates to the technical field of microorganisms. The fungus mainly solves the problems that due to the factors that the liriope spicate steroid saponin is mostly and directly from liriope spicate extracts, the liriope spicate steroid saponin content is low, the saponin extraction cost is high, and the like, the market delivery quantity of the liriope spicate steroid saponin is limited. The aspergillus fungus is separated from living body tuberous roots of liriope spicata var. prolifera, and belongs to aspergillus sp. through 18S rDNA sequence identification; the aspergillus fungus is preserved in CCTCC (China Center for Type Culture Collection); the preservation data is December 7, 2016; the preservation number is CCTCC No.M2016721. The liriope spicate produced by a liriope spicate GAP (Good Agricultural Practices) base (the liriope spicate source areain Oumao town, Xiangyang city in Hubei province ) is used; the liriope spicate steroid saponin is generated through liriope spicate endogenous fungus plant liquid fermentation. The endogenous fungus is an important microorganism for finding a new resource of the liriope spicate steroid saponin.

The invention relates to a medicinal preparation with inhibiting effect on tumor metastasis, which contains steroidal saponin extractives. The effective active components of the medicinal preparation comprise the following eight steroidal saponin general extractives which are eight steroid saponins: steroidal saponin I, steroidal saponin II, dioscin, gracillin, steroidal saponin H, steroidal saponin VII, saponin A (pennogenin-3-O-alpha-L-isodulcitol-yl(1-4)-[alpha-L-isodulcitol-yl(1-2)]-alpha-L-isodulcitol-yl(1-2)-beta-D-heteroside) and saponin B (pennogenin-3-O-alpha-L-isodulcitol-yl(1-4)-[alpha-L-isodulcitol-yl(1-2)]-alpha-L-isodulcitol-yl(1-3)-beta-D-heteroside), and the effective active components of the medicinal preparation also comprise medical dressings. The weight content of the eight steroidal saponin general extractives is 5%-30%, and the weight content of the medical dressings is 70%-95%. The invention is a medicinal preparation with less toxic and side effects and inhibiting effect on tumor metastasis.

The invention relates to a steroid saponin compound in siberian fritillary bulb extracted from a Chinese medicine, i.e., Siberian fritillary bulb, a preparation process thereof and medicinal uses of asthma preventing, cough relieving, phlegm eliminating and the like thereof. A composition can be applied to medicaments, functional foods and foods. The compound is extracted from fritillary plants based on medicinal materials including thunberg fritillary bulb, unibract fritillary bulb, siberian fritillary bulb, hupeh fritillary bulb, ussuriensis fritillary bulb or fritillaria anhuiensis.

The invention belongs to the medicine technology field, particularly relating to extract and separate carbon-21steroidal glycosides mixture from traditional medicine Marsdenia tenacissima. The compound mainly comprises five carbon-21 steroidal glycosides compounds which are respectively tenacissosides A, tenacissosides B, tenacissosides C, tenacissosides D and Marsdenoside K. The mixtures in which the content of the global weight of steroidal glycosides compound is larger or equal to 80% is named as Marsdenia tenacissima total glycoside H. The invention further relates to the preparation process of the compound, a medicine compound which uses the compound as active ingredient, and the application of the compound and the medicine compound of the invention in antitumor agent field.

The invention provides application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin, and relates to the technical field of microorganisms. The application is mainly used for solving the problems that most existing steroid saponin of liriope spicata var.prolifera Y.T.MA is from extracts of liriope spicata var.prolifera Y.T.MA, tubers of liriopespicata var.prolifera Y.T.MA are long in production period, low in steroid saponin content and high in saponin extraction cost, and accordingly the market volume of the steroid saponin of liriope spicata var.prolifera Y.T.MA is limited. The fungus is separated from living tubers of liriope spicata var.prolifera Y.T.MA, authenticated as Cladosporium sp.EF111 by means of the 18 S rDNA sequence, andpreserved in the China Center for Type Culture Collection. The preservation date is December 7th, 2016, and the preservation number of CCTCC No:M2016725. According to the application, liriope spicatavar.prolifera Y.T.MA produced by a liriope spicata var.prolifera Y.T.MA GAP base which is the original production place of liriope spicata var.prolifera Y.T.MA in Oumiao town, Xiangyang city in Hubeiprovince, endophytic fungus strain liquid of liriope spicata var.prolifera Y.T.MA is used for fermentation, and the steroid saponin of liriope spicata var.prolifera Y.T.MA is produced. The endophyticfungus is an important microorganism for finding new resources of steroid saponin of liriope spicata var.prolifera Y.T.MA.

The invention relates to an extracting method of steroid saponins of yerbadetajo, which comprises the following steps: firstly, preparation of coarse extract of yerbadetajo: extracting coarse yerbadetajo powder for 3 times with microwaves by using 70 percent ethanol, recovering the ethanol by two steps to obtain concentrated yerbadetajo extract solution; secondly, preparation of total saponins coarse product of yerbadetajo by adopting a froth counter current chromatography: adding water to dilute the concentrated yerbadetajo extract solution, placing the diluted concentrated yerbadetajo extract solution into a froth counter current chromatographic instrument, continuously extracting by using normal butanol, collecting outflow froth until Libermann reaction is negative, combining the obtained normal butanol, recovering the normal butanol, and obtaining the total saponins coarse product of yerbadetajo; thirdly, purification of the total saponins of yerbadetajo by utilizing macroporous absorbent resin; fourthly, filtration: filtering the obtained total saponins by using a reverse osmosis membrane, further removing water-soluble impurity to obtain the total saponins with high purity. The invention has the advantages of simple and convenient operation, little environmental pollution, small energy consumption, good environmental protection, high extraction efficiency and the like.

An agent selected from A / B-cis furostane, furostene, spirostane and spirostene steroidal sapogenins and ester, ether, ketone and glycosylated forms thereof is used to induce self-regulated homeostasis of neurotrophic factors (NFs), for example BDNF and / or GDNF, NFs with limited and manageable side effects in a subject, by modulating NFs in a non-toxic manner under homeostatic control. An effective amount of at least one such agent is administered to the subject, particularly in the treatment or prevention of a range of NF-mediated disorders, particularly neurological, psychiatric, inflammatory, allergic, immune and neoplastic disorders, and in the restoration or normalisation of neuronal and other function in or in relation to any damaged or abnormal tissue, including when assisting tissue (for example, skin, bone, eye and muscle) healing and general skin, bone, eye and muscle health.

The invention discloses an extraction process of a rhizoma polygonati extract, relating to the technical field of rhizoma polygonati extraction. The extraction process comprises the following steps: (1) pretreatment of rhizoma polygonati; (2) extraction of rhizoma polygonati saponin; (3) purification of the rhizoma polygonati saponin; (4) extraction of rhizoma polygonati polysaccharide; (5) decoloration of a rhizoma polygonati polysaccharide extracting solution; and (6) purification of the rhizoma polygonati polysaccharide. By the modes of alcohol extraction and water extraction, steroid saponin and polysaccharide in the rhizoma polygonati are extracted multiple times so as to extract active ingredients in the rhizoma polygonati as far as possible, and then purified and separated by self-made adsorption resin, not only is the purity of the active ingredients improved, but also the yield of the active ingredients is increased.

The present invention relates to a Chinese medicine tupistra chinensis extract, its preparation method and application. Said extract is obtained by extracting root, stem and leaf of tupistra chinensis, and is a brownish yellow powder. The characteristics of its chemical identification are as follows: Liebermann-Burchard reaction and Molish reaction are positive; the foam test is positive reaction, the foam height of alkaline liquor tube is higher than that of acid liquor tube; Rosen-Heimer reaction at 60deg.C can produce violet red color reaction, it is a steroid saponin compound. Said extract has the action of resisting tumor, resisting inflammation, inhibiting platelet agglutination and resisting thrombosis.

The invention provides a UPLC detection method for various steroidal saponins in Chonglou yunnanensis or its multi-bud strains. The above detection method is simple, accurate, short in analysis time and good in stability, and can be used for detection of steroidal saponins in Chonglou yunnanensis or its multi-bud strains.

Vincetoxin, its saponin compound and use in antiphlogistic medicine are disclosed. The vincetoxin consists of bine vincetoxin A, bine vincetoxin B, bine vincetoxin C, bine vincetoxin D, vincetoxicoside C-3-O-beta-D-thevetoside, vincetoxicoside C, vincetoxicoside H, atratoglaucosides A and cynanosides I. Vincetoxin is mixture which contains any kinds of the nine compounds with content >50%. It has excellent inhibiting function for various inflammations and can be used to treat chronic pharyngitis, chronic enterogastritis, acute tonsillitis and upper respiratory infection.

The invention discloses certain steroidal sapogenins and derivatives thereof, and their use in the treatment of cognitive dysfunction, non-cognitive neurodegeneration, non-cognitive neuromuscular degeneration, and receptor loss in the absence of cognitive, neural and neuromuscular impairment. Methods of synthesis, treatment and pharmaceutical compositions are also disclosed.

The invention provides a hydrophobic steroid saponin extract of peltate yam rhizome as well as a preparation method and use thereof. In the invention, in vitro anti-cancer drug experimental study proves that the hydrophobic steroid saponin extract of the peltate yam rhizome has strong growth inhibition activity on cell lines of colon cancer, lung cancer, breast cancer, cervical carcinoma, ovarian cancer, larynx cancer and the like. The extract of peltate yam rhizome has the advantages of wide resource, simple extraction and separation process of saponin, and wide prospect in cancer treatment, thus developing a new anticancer use of a hydrophobic dioscin component in the peltate yam rhizome.

The invention relates to a preparation method of the extraction of total steroid saponins of longstamen onion bulb, which is as follow: cutting the longstamen onion bulb into pieces; adding ethanol for lixiviation or reflux extraction; combining extraction liquid; decompression and concentration; quiescence or centrifugation; taking and diluting the lower sediment with water; extracting with water saturated normal butanol and then decompressing and concentrating until no liquid; dissolving the residue with ethanol and adding acetone until no production of sediment; filtering and drying to obtain the extraction of total steroid saponins. The preparation method has the advantages of rational design, simple technology, low production cost, high yield, high purity of product. The primary study of biological activities shows that the extraction of total steroid saponins has obvious protection effect for the myocardial cell trauma induced by H2O2, and can be used for the preparation of medicines with obvious protection effect for myocardial cell trauma.

The invention discloses Xiangyang radix ophiopogonis endophytic penicillium oxalicum penicillium fungus and application in steroid saponin preparation, and relates to the technical field of microorganisms. The problems that Xiangyang radix ophiopogonis steroid saponin is mostly directly from Xiangyang radix ophiopogonis extractives, and the Xiangyang radix ophiopogonis steroid saponin is limited in market delivery quantity due to the fact that Xiangyang radix ophiopogonis is low in steroid saponin content and high in saponin extraction cost are solved. The penicillium fungus is separated fromXiangyang radix ophiopogonis (Liriope spicata var.proliferaY.T.Ma) living earthnuts, it is determined as penicillium sp. EF050 through 18S rDNA sequence authentication, the penicillium fungus is preserved in the China Center for Type Culture Collection, the preservation date is 20th December, 2016, and the preservation number is CCTCC No:M2016766. By means of Xiangyang radix ophiopogonis producedin a Xiangyang radix ophiopogonis GAP base-the source area of Xiangyang radix ophiopogonis in Oumiao county, Xiangyang city, Hubei province, due to fermentation of Xiangyang radix ophiopogonis endophytic fungi strain liquid, the Xiangyang radix ophiopogonis steroid saponin is produced. The endophytic fungi are the important microorganism for searching for new resources of the Xiangyang radix ophiopogonis steroid saponin.

The invention discloses a solanum lyratum extract, and an anticancer medicine comprising the solanum lyratum extract. The solanum lyratum extract is prepared through steps that: a solanum lyratum medicinal material is extracted by using ethanol, wherein the solanum lyratum medicinal material is processed through ethanol refluxing extraction, the recovered solvent is evaporated until dried, such that an ethanol extract is obtained; the extract is refined, wherein the ethanol extract is dissolved in water to form a solution, the solution is absorbed by using a weak acidic cation exchange macroporous resin column and is orderly eluted by using ethanol and hydrochloric ethanol, the hydrochloric ethanol eluent is collected and is neutralized by using ammonia water, and the collected product isdesalted, such that the solanum lyratum extract is prepared. The solanum lyratum extract contains 1% to 60% of steroid alkaloid. According to the invention, weak acidic cation exchange macroporous resin is adopted, steroid alkaloid is specifically accumulated from various components of solanum lyratum. The component of accumulated steroid alkaloid is simple. As a result of experiments, the substance has a substantial anticancer potency, and the anticancer potency is greatly higher than that of solanum lyratum aqueous crude extracts and steroid saponins extracted by using n-butyl alcohol in prior arts.

The present invention relates to steriod saponin from sealwort as Chinese medicine material and its medical use and preparation process. It has the effects of improving cerebral function and lowering blood sugar level. It is prepared with sealwort root powder and through water extraction, concentration, chloroform extraction, macroporous resin adsorption, dilute alcohol washing to eliminate impurity, alcohol elution to obtain alcohol solution, recovering solvent to obtain steriod saponin, silica gel chromatography to separate, re-crystallization, and HPLC chromatography to obtain triterpenoid saponin monomer. The present invention is suitable for industrial production and has the functions of improving learning and memory capacity of early stage senile dementia.

The present invention discloses a caltrop total steroid saponin which comprises caltrop total spirostanol saponin and caltrop total furostanol saponin and is characterized in that the content of the caltrop total furostanol saponin is more than 50 percent (W / W), which can meet the requirement of developing more than five kinds of new traditional Chinese medicines. The present invention also discloses a preparation method for the caltrop total steroid saponin, which has the following steps of (1) extracting ethanol; (2) filtering and concentrating; (3) absorbing macroporous resin; (4) washing pillar; (5) concentrating; (6) drying; (7) crushing, and the caltrop total steroid saponin can be obtained. The macroporous resin absorption means absorbing and concentrating the macroporous resin having the aperture of 90A degrees to 100A degrees and the specific surface area of 500m<2> / g to 550 m<2> / g above the filtrate of concentrated solution to obtain a midbody. The preparation method is simple; corresponding production equipments can be obtained easily; the production technology is grasped easily, which is especially suitable for industrialized mass production. The present invention collects the eluate with the ethanol having corresponding concentration by one step alcohol extracting-water precipitating and then concentrating and absorbing the macroporous resin for condensing, drying and crushing to obtain the caltrop total furostanol saponin.

The invention discloses an application of a cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel. The prepared cuckoopint steroid saponin effective component is one or more of pennogenin, pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside, pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->4)-[alpha-L- rhamnopy ranosyl(1->2)-beta-D-glucoside and pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->4)-alpha-L- rhamnopy ranosyl(1->4)- alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside. For thecuckoopint steroid saponin effective component has the common structure of pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside, the composition has the similar treatment effect in a human body; the composition is mainly applied to the clinical drugs for treating the cerebral hemorrhage or cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel caused by cardiocerebral vascular system disease.

The invention relates to C27 spirostane-type steroidal saponins isolated from Ypsilandra, Trillium and Paris and their preparation method, as well as their application in the preparation of drugs for treating hemorrhagic diseases, and application in the preparation of drugs for treating dysfunctional uterine bleeding, and the preparation of functional daily chemical products and health products. The materials used in a method of the invention are easy to obtain, the method is simple and easily operable, biological experiments of the compound produced show that the compound can induce platelet aggregation activity and hemostatic activity significantly.

The invention belongs to the technical field of medicine, and in particular relates to steroid sapogenin derivatives and a pharmaceutical composition and application thereof in preparing antithrombotic medicaments. The derivatives provided by the invention have stronger activity than that of the existing steroid saponin medicaments, the quality is easy to control, the activity is equivalent to that of the existing anti-platelet aggregation medicaments, but the derivatives provided by the invention have no side effects on the gastrointestinal tract, thus being applicable to patients intolerantto the anti-platelet aggregation medicaments.

The invention provides a Radix asparagi steroid saponin extract and its preparing process and use thereof, wherein six compounds are isolated from the asparagi steroid saponin extract, among which four of them are novel compounds, the preparing process includes n-butyl alcohol extraction method and macroporous resin absorption method.

The present invention relates to the new process of synthesizing furasteroid saponin and its derivative chemically with C27 helical sterane type sapogenin and various kinds of saccharide as material. The synthesis process includes the following steps: 1. synthesizing completely protected C27 helical steroid saponin through the reaction of C27 helical sterane type sapogenin and acyl radical, alkyl radical or silicon radical protected glycosyl donor; 2. reaction of completely protected helical steroid saponin and oxidant to obtain oxidized product; 3. reaction of oxidized helical steroid saponin and oxyphilic reagent to produce place 26 halogenated saponin derivative; 4. nucleophilic substitution reaction of nucleophilic reagent and the halogenated product to introduce XR3 radical; 5. reducing the place 16 carbonyl radical in saponin selectively with reductant; and 6. eliminating all the protecting radicals to obtain furasteroid saponin and its analog.

The invention belongs to the technical field of medicines, and relates to a new steroid saponin type compound extracted and separated out from roots and tubers of trillium tschonoskii, and application of the new steroid saponin type compound to preparation of an inflammatory cell inhibitor. The compound has a chemical structural formula shown as a formula I; an experiment result shows that the new steroid saponin type compound can be used for inhibiting generation of an inflammatory mediator NO in mouse monocyte-macrophages RAW264.7 under the stimulation of LPS (Lipopolysaccharide); a preparation method of the compound is simple and easy, and further pharmacological and clinical researches are easy to carry out; the application of the new steroid saponin type compound in anti-inflammatory drugs is developed. (The formula I is shown in the description.).

The invention relates to a pharmacological composition based on biologically active substances obtained from leaves, stems and fruit of the plant Tribulus terrestris L. to be used as an agent strengthening the immune system, increasing T-cells and anti-bodies number and testosterone quantity in human organism and having a strong anti-virus and anti-inflammatory effect in the form of a medicine or as an addition to food. The pharmacological composition represents a combination of bio-active zinc, bio-active selenium and a complex of steroid saponins obtained from leaves, stems and fruit of the plant Tribulus terrestris l. and consisting of furostanols spirostanols, sapogenins, sterols, flavonoids and other biologically active substances typical of this plant.

The invention discloses an anemarrhena asphodeloides bge steroid saponin compound, and a preparation method and an application thereof in preparation of an antitumor drug. The compound is spirostanolsteroid saponin, the molecular formula of the compound is C45H74O17, and the molecular weight of the compound is 886. The compound is prepared by extracting medicinal plant anemarrhena asphodeloides bge with 75% ethanol, extracting with water-saturated n-butyl alcohol, and separating and purifying by macroporous adsorption resin, silica gel column chromatography, ODS reversed-phase column chromatography, gel column chromatography and other methods. MTT experimental results show that the compound has remarkable anti-tumor activity on human breast cancer cell strains (MCF-7) and human liver cancer cell strains (HepG2), and is expected to become an anti-tumor candidate drug.

The invention relates to a pharmacological substance based on biologically active substances obtained from the plant Tribulus terrestris L. to be used as an agent reducing blood sugar, improving blood circulation and especially blood circulation in veins and capillaries of the limbs of diabetic patients, reducing bad cholesterol level, increasing good cholesterol concentration, maintaining cardiovascular and liver functioning with an additional prophylactic or healing effect on the immune system and the immune resistance. The pharmacological substance represents a combination of bio-active trivalent chromium and a complex of steroid saponins obtained from the plant Tribulus Terrestris l. and consisting of furostanols, spirostanols, sapogenins, sterols, flavonoids and other biologically active substances typical of this plant.