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UPLC (ultra-performance liquid chromatography) detection method of multiple steroid saponins in Yunnan rhizoma paridis or its polygerm strain

A detection method and technology for steroidal saponins, which are applied in the field of UPLC detection of various steroidal saponins, can solve the problems of few research reports on quality evaluation, achieve stable and reliable sample addition and recovery test, shorten separation and analysis time, and repeatability. Stable and reliable effect

Inactive Publication Date: 2017-01-25
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More and more attention has been paid to the multi-bud strain of Chonglou yunnanensis with high yield and high reproduction rate, but there are few research reports on its quality evaluation

Method used

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  • UPLC (ultra-performance liquid chromatography) detection method of multiple steroid saponins in Yunnan rhizoma paridis or its polygerm strain
  • UPLC (ultra-performance liquid chromatography) detection method of multiple steroid saponins in Yunnan rhizoma paridis or its polygerm strain
  • UPLC (ultra-performance liquid chromatography) detection method of multiple steroid saponins in Yunnan rhizoma paridis or its polygerm strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1 Apparatus and materials

[0051] Waters Acquity H-Class ultra-high performance liquid chromatograph (Waters, USA); KQ-5200E ultrasonic cleaner (40kHz, 250W, Kunshan Ultrasonic Instrument Co., Ltd.); BP211D electronic balance (Sartorius, Germany); METTLER AE240 electronic analytical balance ( Shanghai Mettler-Toledo Instruments Co., Ltd.). Acetonitrile is chromatographically pure (American Sigma Company); water is Watsons distilled water; methanol is analytically pure (Chengdu Kelon Chemical Reagent Factory).

[0052] Saponins I (batch number: MUST-15061611, Purity: 98.34%), Ⅱ (batch number: MUST-15060801, Purity: 98.45%), VI (batch number: MUST-14071904, Purity: 98.67%), Ⅶ (batch number: MUST -14060302, Purity: 99.03%), Dioscorea quinquefolium saponins (batch number: MUST-15081110, Purity: 98.86%) were purchased from Chengdu Master Biotech Co., Ltd.; Chonglou saponin H and Chonglou saponin V were purchased from Sichuan University West China Pharmaceutical The pharmacog...

Embodiment 2

[0085] (1) Take the dried Yunnan Chonglou multi-bud strain to be tested, pulverize, extract with methanol solvent, combine the extracts, and reconstitute the residue after removing the solvent with methanol to prepare the test solution;

[0086] (2) Take the mixture of saponins Ⅰ, Ⅱ, Ⅴ, Ⅵ, Ⅶ, H and Dioscorea nigra to prepare a reference solution;

[0087] (3) Inject the test solution and the reference solution into the ultra-high performance liquid chromatograph, and use the external standard method for detection; the chromatographic conditions are as follows:

[0088] Column: CQUITY BEH C18 chromatographic column, 2.1mm×50mm, 1.7μm;

[0089] ELSD detector conditions: drift tube temperature 55℃, nitrogen pressure 40psi, gain 500, cooling mode;

[0090] Mobile phase: acetonitrile (A)-water (B) system, the gradient program is as follows:

[0091] 0~1min 5%~25%A; 1~2.5min, 25%~30%A; 2.5~4min, 30%~35%A; 4~6min, 35%~40%A; 6~9min, 40% A~45%A; 9~21min, 45%A~50%A; 21~22min, 50%A~70%A; 22~23mi...

Embodiment 3

[0095] (1) Take the dried Yunnan Chonglou multi-bud strain to be tested, pulverize, extract with methanol solvent, combine the extracts, and reconstitute the residue after removing the solvent with methanol to prepare the test solution;

[0096] (2) Take the mixture of saponins Ⅰ, Ⅱ, Ⅴ, Ⅵ, Ⅶ, H and Dioscorea nigra to prepare a reference solution;

[0097] (3) Inject the test solution and the reference solution into the ultra-high performance liquid chromatograph, and use the external standard method for detection; the chromatographic conditions are as follows:

[0098] Column: CQUITY BEH C18 chromatographic column, 2.1mm×50mm, 1.7μm;

[0099] ELSD detector conditions: drift tube temperature 55℃, nitrogen pressure 40psi, gain 500, cooling mode;

[0100] Mobile phase: acetonitrile (A)-water (B) system, the gradient program is as follows:

[0101] 0~1min 10%~25%A; 1~2.5min, 25%~30%A; 2.5~4min, 30%~35%A; 4~6min, 35%~40%A; 6~8min, 40% A~45%A; 8~21min, 45%A~50%A; 21~23min, 50%A~90%A. Flow ...

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Abstract

The invention provides a UPLC detection method for various steroidal saponins in Chonglou yunnanensis or its multi-bud strains. The above detection method is simple, accurate, short in analysis time and good in stability, and can be used for detection of steroidal saponins in Chonglou yunnanensis or its multi-bud strains.

Description

Technical field [0001] The invention relates to the field of quality detection of Chinese medicinal materials, in particular to a UPLC detection method for various steroidal saponins in Yunnan Chonglou or its multi-bud strains. Background technique [0002] The first edition of the Pharmacopoeia of the People’s Republic of China in the 2015 edition contains the species of Liliaceae Parispolyphylla Smith var.yunnanensis (Franch.) Hand.-Mazz. or Parispolyphylla Smith var.chinensis (Franch.) Hara The dry rhizomes have the effects of clearing heat and detoxification, reducing swelling and pain, cooling the liver and relieving shock. Modern studies have shown that Chonglou has anti-tumor, hemostasis, immune regulation, sedation, and analgesic effects, and steroidal saponins are the main active ingredients that play a role. In recent years, due to the huge market demand for heavy buildings, endangered wild resources, high requirements for artificial breeding technology, and long culti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘圆张绍山黄钟杰余孟杰
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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